Fam135a gene cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA	Protein	Name
XM_008856282.1	XP_008854504.1	protein FAM135A isoform X2
XM_008856285.1	XP_008854507.1	protein FAM135A isoform X4
XM_008856285.2	XP_008854507.1	protein FAM135A isoform X8
XM_029557803.1	XP_029413663.1	protein FAM135A isoform X2
XM_017795165.1	XP_017650654.1	protein FAM135A isoform X3
XM_017795166.1	XP_017650655.1	protein FAM135A isoform X5
XM_017795164.2	XP_017650653.1	protein FAM135A isoform X1
XM_029557807.1	XP_029413667.1	protein FAM135A isoform X4
XM_029557809.1	XP_029413669.1	protein FAM135A isoform X7
XM_029557811.1	XP_029413671.1	protein FAM135A isoform X8
XM_029557804.1	XP_029413664.1	protein FAM135A isoform X2
XM_029557805.1	XP_029413665.1	protein FAM135A isoform X2
XM_017795165.2	XP_017650654.1	protein FAM135A isoform X5
XM_008856282.2	XP_008854504.1	protein FAM135A isoform X3
XM_017795164.1	XP_017650653.1	protein FAM135A isoform X1
XM_029557806.1	XP_029413666.1	protein FAM135A isoform X4
XM_029557808.1	XP_029413668.1	protein FAM135A isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa138120	XM_029557808.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ONa138121	XM_029557809.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa38694	XM_017795165.2 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONa38695	XM_008856285.2 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa38695	XM_029557811.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa38696	XM_017795166.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa138118	XM_029557803.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ONa138118	XM_029557804.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ONa138118	XM_029557805.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ONa138119	XM_029557806.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ONa138119	XM_029557807.1 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ONa38692	XM_017795164.2 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ONa38693	XM_008856282.2 Latest version!	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
ONa38695	XM_008856285.1	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa38694	XM_017795165.1	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONa38693	XM_008856282.1	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ONa38692	XM_017795164.1	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

Fam135a ( XM_029557808.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413668.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ONa138120
Clone ID Related Accession (Same CDS sequence)	XM_029557808.1
Accession Version	XM_029557808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGGTAA ACTTGGTATC TCCCAGGACG ATAGTGATAT CACACACATG 
GAACATAATC TGGCATCAAG AAGTTCATCA GATGATTTCC ATGACTGTCA GACAACCCCA 
TCTTCAGCAG TTAAAACACT TGAAGTAAAT TCCACCAATA AAAATCCTTT CGATGGAGAG 
AAAATAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTAGAT 
AATTTTCTAT CCACTTTTGA ATCCTTAGAC TCTAATGGTA AATCCAAGTC TATAGAAATA 
ACACTTGAAA AGGAGGCTTT ACAGGAAGCA AAATGTCATT CTTTTGGAGA ATCATTAGCT 
AAATTAAGAA ATAATCTACC TGCTCCTACA AAAGAATATC ATGTTGTAGT AAGTGGAGAT 
ACAATTAAAT TACCAGATAC TAATGCCACA TATGCCTCAT CTAGATTTTC AGATTCAGGT 
GTTGAAAGTG AACCAAGCTC TTTTGCAACA CATCCAAACC TGGAAATAGT CTCTGAAACT 
GGCCAAGGGC AAGGTCCTTA CCATAATGAA AGGTTATTTC CTCAGCTTTT GATGAAACCT 
GATTACAATG TCAAGTTTTC ATTAGGAAGT CACTGTACTG AGAGTACAAG TGCTTTAAGT 
GAAATACAGT CATCTTTGAC ATCAATAAAC TCTCTACCTT CTGATGATGA ACTCTCACCT 
GATGAAAATT CTAAGAAATC TTCTGTGCCT GAATGCCATC TAAGTGATAG TAAAACTGTA 
TTAAATCTAG GAACAATGGA TTTGCCAAAG TGTGATGATA CTAAAAAGTC AAGTATTATT 
TTGCAGCCTC AATGTGTTGT ATTTTCAGGG CATTTGGACA ATGAAACTTT AGCAATACAT 
TCCTTAAATT CAAGCACTGA AGACCCTTTA CAACATGTTT TTTCAGATAA AGATACTTCT 
AGTGATGTGA AAAGTAGTTG TAGCTCCCAG CCTAATTTGG ACCCTATATT TAAAGGCTCC 
CAGAGTCATG ATACATCTAA TAACAACTCT GCAGGAACCG TGATCGCAAT ACAGTCAGAA 
CTGATTTGCT TAGGCACTCC TTGTGTTGTT TCGGGTTCTG TTTCTACTAA TTCAGAACTA 
AGTCAAGAGG GAACTGTGGA AAGAAAAAGT AGTGACCCAT TAAGTCTCAA ACAAATGTAT 
TCAGAAGCCC CTTCAATTGA ACATGAACTT CATCTGGGTT CGAGTGACCC TCTTTCAGCC 
AGTACTGATA TGGTAAAAGA AGGGCTTGTG GAAAATTATT TTGGTTCTCA GAGCAGTACA 
GATATTTCGG ACATACGTGC TGTTAGCTAC CACAACTCAG TTAGCCCTCA GAAGGAAACC 
TGTGAAAAAG GAATCAGTGA CCTTCAGCAG GAACAGGGCA AAGATGAGGA GGAGGAAGAA 
CAGGATCAAC AAATGATTCA AAATGGGTAT TATGAAGAAA CAGATTTTTC AGCTTTGGGT 
GGAACTATAA ATGCTCACTT TCCCAGCAGA GATGTGCAAA CAGAGGAAAG GCATCTAAAA 
TCTGAAGAAG TGAACAGTGA CTATCTGAGG GACAGTGTGA ATGTGCCTAC TGTTTGTACT 
TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA CGAGAGTCTC CTTGTAGTGT TAAATACTCT 
TCTAGAAGTA AATTTGATGC CATTACAAAG CAGCCAAGCA GCATTTCTTA CAACTTCACT 
TCTTCAATTT CCTGGTATGA AAACTCACCA AAACCTCAAA TACAAGCCTT CCTTCAGGCA 
AAAGAAGAAC TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGTGATGT TCCTCTGCTG 
GCATCCTCAG TACCTTATTT CAGCATGGAA GAAGAAGATG GTTCTGAAGA TGGTGTCCAT 
CTCATTGTCT GCGTGCATGG TTTAGATGGT AACAGTGCAG ATCTCCGACT AGTAAAAACG 
TATATTGAAC TTGGACTGCC TGGGGGAAGA GTTGATTTTC TTATGTCTGA GAGAAATCAG 
AATGATACTT TCGCTGATTT TGATAGCATG ACTGATCGTC TTTTGGATGA AATCATACAG 
TATATTCAGA TATATAGTTT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCATTGGGC 
AATTTAATAA TCCGTTCAGT GCTCACAAGG CCACGGTTTA AATATTACCT CAGCAAACTT 
CATACCTTTC TCTCTCTTTC TGGGCCTCAC CTTGGTACAC TGTACAATAG CAGTGCTCTC 
GTCAATACAG GTCTCTGGTT CATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTGCAACTG 
ACATGTCGAG ATCATTCAGA CCCTCGCCAA ACTTTTTTAT ATAAGCTTAG TAATAAAGCA 
GGGCTTCATT ATTTCAAGAA TGTTGTGCTG GTAGGATCAC TACAGGATCG CTATGTTCCA 
TATCATTCTG CCCGCATTGA AATGTGCAAA ACAGCCTTGA AGGACAAACA GTCAGGACAA 
ATCTATTCAG AAATGATCCA CAACTTGCTT CGCCCAGTTC TGCAAAGCAA AGACTGTAAT 
TTGATTCGAT ACAATGTCAT CAATGCATTG CCCAACACGG CTGATTCACT CATCGGGAGA 
GCTGCACACA TAGCTGTTCT TGATTCGGAA ATATTTTTAG AGAAGTTCTT CCTGGTTGCT 
GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413668.1
CDS	526..4566
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557808.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557808.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGGTAA ACTTGGTATC TCCCAGGACG ATAGTGATAT CACACACATG 
GAACATAATC TGGCATCAAG AAGTTCATCA GATGATTTCC ATGACTGTCA GACAACCCCA 
TCTTCAGCAG TTAAAACACT TGAAGTAAAT TCCACCAATA AAAATCCTTT CGATGGAGAG 
AAAATAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTAGAT 
AATTTTCTAT CCACTTTTGA ATCCTTAGAC TCTAATGGTA AATCCAAGTC TATAGAAATA 
ACACTTGAAA AGGAGGCTTT ACAGGAAGCA AAATGTCATT CTTTTGGAGA ATCATTAGCT 
AAATTAAGAA ATAATCTACC TGCTCCTACA AAAGAATATC ATGTTGTAGT AAGTGGAGAT 
ACAATTAAAT TACCAGATAC TAATGCCACA TATGCCTCAT CTAGATTTTC AGATTCAGGT 
GTTGAAAGTG AACCAAGCTC TTTTGCAACA CATCCAAACC TGGAAATAGT CTCTGAAACT 
GGCCAAGGGC AAGGTCCTTA CCATAATGAA AGGTTATTTC CTCAGCTTTT GATGAAACCT 
GATTACAATG TCAAGTTTTC ATTAGGAAGT CACTGTACTG AGAGTACAAG TGCTTTAAGT 
GAAATACAGT CATCTTTGAC ATCAATAAAC TCTCTACCTT CTGATGATGA ACTCTCACCT 
GATGAAAATT CTAAGAAATC TTCTGTGCCT GAATGCCATC TAAGTGATAG TAAAACTGTA 
TTAAATCTAG GAACAATGGA TTTGCCAAAG TGTGATGATA CTAAAAAGTC AAGTATTATT 
TTGCAGCCTC AATGTGTTGT ATTTTCAGGG CATTTGGACA ATGAAACTTT AGCAATACAT 
TCCTTAAATT CAAGCACTGA AGACCCTTTA CAACATGTTT TTTCAGATAA AGATACTTCT 
AGTGATGTGA AAAGTAGTTG TAGCTCCCAG CCTAATTTGG ACCCTATATT TAAAGGCTCC 
CAGAGTCATG ATACATCTAA TAACAACTCT GCAGGAACCG TGATCGCAAT ACAGTCAGAA 
CTGATTTGCT TAGGCACTCC TTGTGTTGTT TCGGGTTCTG TTTCTACTAA TTCAGAACTA 
AGTCAAGAGG GAACTGTGGA AAGAAAAAGT AGTGACCCAT TAAGTCTCAA ACAAATGTAT 
TCAGAAGCCC CTTCAATTGA ACATGAACTT CATCTGGGTT CGAGTGACCC TCTTTCAGCC 
AGTACTGATA TGGTAAAAGA AGGGCTTGTG GAAAATTATT TTGGTTCTCA GAGCAGTACA 
GATATTTCGG ACATACGTGC TGTTAGCTAC CACAACTCAG TTAGCCCTCA GAAGGAAACC 
TGTGAAAAAG GAATCAGTGA CCTTCAGCAG GAACAGGGCA AAGATGAGGA GGAGGAAGAA 
CAGGATCAAC AAATGATTCA AAATGGGTAT TATGAAGAAA CAGATTTTTC AGCTTTGGGT 
GGAACTATAA ATGCTCACTT TCCCAGCAGA GATGTGCAAA CAGAGGAAAG GCATCTAAAA 
TCTGAAGAAG TGAACAGTGA CTATCTGAGG GACAGTGTGA ATGTGCCTAC TGTTTGTACT 
TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA CGAGAGTCTC CTTGTAGTGT TAAATACTCT 
TCTAGAAGTA AATTTGATGC CATTACAAAG CAGCCAAGCA GCATTTCTTA CAACTTCACT 
TCTTCAATTT CCTGGTATGA AAACTCACCA AAACCTCAAA TACAAGCCTT CCTTCAGGCA 
AAAGAAGAAC TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGTGATGT TCCTCTGCTG 
GCATCCTCAG TACCTTATTT CAGCATGGAA GAAGAAGATG GTTCTGAAGA TGGTGTCCAT 
CTCATTGTCT GCGTGCATGG TTTAGATGGT AACAGTGCAG ATCTCCGACT AGTAAAAACG 
TATATTGAAC TTGGACTGCC TGGGGGAAGA GTTGATTTTC TTATGTCTGA GAGAAATCAG 
AATGATACTT TCGCTGATTT TGATAGCATG ACTGATCGTC TTTTGGATGA AATCATACAG 
TATATTCAGA TATATAGTTT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCATTGGGC 
AATTTAATAA TCCGTTCAGT GCTCACAAGG CCACGGTTTA AATATTACCT CAGCAAACTT 
CATACCTTTC TCTCTCTTTC TGGGCCTCAC CTTGGTACAC TGTACAATAG CAGTGCTCTC 
GTCAATACAG GTCTCTGGTT CATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTGCAACTG 
ACATGTCGAG ATCATTCAGA CCCTCGCCAA ACTTTTTTAT ATAAGCTTAG TAATAAAGCA 
GGGCTTCATT ATTTCAAGAA TGTTGTGCTG GTAGGATCAC TACAGGATCG CTATGTTCCA 
TATCATTCTG CCCGCATTGA AATGTGCAAA ACAGCCTTGA AGGACAAACA GTCAGGACAA 
ATCTATTCAG AAATGATCCA CAACTTGCTT CGCCCAGTTC TGCAAAGCAA AGACTGTAAT 
TTGATTCGAT ACAATGTCAT CAATGCATTG CCCAACACGG CTGATTCACT CATCGGGAGA 
GCTGCACACA TAGCTGTTCT TGATTCGGAA ATATTTTTAG AGAAGTTCTT CCTGGTTGCT 
GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_029557809.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413669.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ONa138121
Clone ID Related Accession (Same CDS sequence)	XM_029557809.1
Accession Version	XM_029557809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGATAAA 
ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG GTAAACTTGG TATCTCCCAG GACGATAGTG ATATCACACA CATGGAACAT 
AATCTGGCAT CAAGAAGTTC ATCAGATGAT TTCCATGACT GTCAGACAAC CCCATCTTCA 
GCAGTTAAAA CACTTGAAGT AAATTCCACC AATAAAAATC CTTTCGATGG AGAGAAAATA 
ACTGTTAAAA TAGGACCTTG GACAGAGCTT CAACAAGATG AAATATTTGT AGATAATTTT 
CTATCCACTT TTGAATCCTT AGACTCTAAT GGTAAATCCA AGTCTATAGA AATAACACTT 
GAAAAGGAGG CTTTACAGGA AGCAAAATGT CATTCTTTTG GAGAATCATT AGCTAAATTA 
AGAAATAATC TACCTGCTCC TACAAAAGAA TATCATGTTG TAGTAAGTGG AGATACAATT 
AAATTACCAG ATACTAATGC CACATATGCC TCATCTAGAT TTTCAGATTC AGGTGTTGAA 
AGTGAACCAA GCTCTTTTGC AACACATCCA AACCTGGAAA TAGTCTCTGA AACTGGCCAA 
GGGCAAGGTC CTTACCATAA TGAAAGGTTA TTTCCTCAGC TTTTGATGAA ACCTGATTAC 
AATGTCAAGT TTTCATTAGG AAGTCACTGT ACTGAGAGTA CAAGTGCTTT AAGTGAAATA 
CAGTCATCTT TGACATCAAT AAACTCTCTA CCTTCTGATG ATGAACTCTC ACCTGATGAA 
AATTCTAAGA AATCTTCTGT GCCTGAATGC CATCTAAGTG ATAGTAAAAC TGTATTAAAT 
CTAGGAACAA TGGATTTGCC AAAGTGTGAT GATACTAAAA AGTCAAGTAT TATTTTGCAG 
CCTCAATGTG TTGTATTTTC AGGGCATTTG GACAATGAAA CTTTAGCAAT ACATTCCTTA 
AATTCAAGCA CTGAAGACCC TTTACAACAT GTTTTTTCAG ATAAAGATAC TTCTAGTGAT 
GTGAAAAGTA GTTGTAGCTC CCAGCCTAAT TTGGACCCTA TATTTAAAGG CTCCCAGAGT 
CATGATACAT CTAATAACAA CTCTGCAGGA ACCGTGATCG CAATACAGTC AGAACTGATT 
TGCTTAGGCA CTCCTTGTGT TGTTTCGGGT TCTGTTTCTA CTAATTCAGA ACTAAGTCAA 
GAGGGAACTG TGGAAAGAAA AAGTAGTGAC CCATTAAGTC TCAAACAAAT GTATTCAGAA 
GCCCCTTCAA TTGAACATGA ACTTCATCTG GGTTCGAGTG ACCCTCTTTC AGCCAGTACT 
GATATGGTAA AAGAAGGGCT TGTGGAAAAT TATTTTGGTT CTCAGAGCAG TACAGATATT 
TCGGACATAC GTGCTGTTAG CTACCACAAC TCAGTTAGCC CTCAGAAGGA AACCTGTGAA 
AAAGGAATCA GTGACCTTCA GCAGGAACAG GGCAAAGATG AGGAGGAGGA AGAACAGGAT 
CAACAAATGA TTCAAAATGG GTATTATGAA GAAACAGATT TTTCAGCTTT GGGTGGAACT 
ATAAATGCTC ACTTTCCCAG CAGAGATGTG CAAACAGAGG AAAGGCATCT AAAATCTGAA 
GAAGTGAACA GTGACTATCT GAGGGACAGT GTGAATGTGC CTACTGTTTG TACTTCGGGT 
TGTTTGTCTT TCCCTTCTGC TCCACGAGAG TCTCCTTGTA GTGTTAAATA CTCTTCTAGA 
AGTAAATTTG ATGCCATTAC AAAGCAGCCA AGCAGCATTT CTTACAACTT CACTTCTTCA 
ATTTCCTGGT ATGAAAACTC ACCAAAACCT CAAATACAAG CCTTCCTTCA GGCAAAAGAA 
GAACTGAAGC AACTTAAACT CCCTGGGTTC ATGTACAGTG ATGTTCCTCT GCTGGCATCC 
TCAGTACCTT ATTTCAGCAT GGAAGAAGAA GATGGTTCTG AAGATGGTGT CCATCTCATT 
GTCTGCGTGC ATGGTTTAGA TGGTAACAGT GCAGATCTCC GACTAGTAAA AACGTATATT 
GAACTTGGAC TGCCTGGGGG AAGAGTTGAT TTTCTTATGT CTGAGAGAAA TCAGAATGAT 
ACTTTCGCTG ATTTTGATAG CATGACTGAT CGTCTTTTGG ATGAAATCAT ACAGTATATT 
CAGATATATA GTTTAACAGT CTCAAAAATA AGCTTTATTG GACATTCATT GGGCAATTTA 
ATAATCCGTT CAGTGCTCAC AAGGCCACGG TTTAAATATT ACCTCAGCAA ACTTCATACC 
TTTCTCTCTC TTTCTGGGCC TCACCTTGGT ACACTGTACA ATAGCAGTGC TCTCGTCAAT 
ACAGGTCTCT GGTTCATGCA GAAATGGAAA AAATCAGGTT CTCTTTTGCA ACTGACATGT 
CGAGATCATT CAGACCCTCG CCAAACTTTT TTATATAAGC TTAGTAATAA AGCAGGGCTT 
CATTATTTCA AGAATGTTGT GCTGGTAGGA TCACTACAGG ATCGCTATGT TCCATATCAT 
TCTGCCCGCA TTGAAATGTG CAAAACAGCC TTGAAGGACA AACAGTCAGG ACAAATCTAT 
TCAGAAATGA TCCACAACTT GCTTCGCCCA GTTCTGCAAA GCAAAGACTG TAATTTGATT 
CGATACAATG TCATCAATGC ATTGCCCAAC ACGGCTGATT CACTCATCGG GAGAGCTGCA 
CACATAGCTG TTCTTGATTC GGAAATATTT TTAGAGAAGT TCTTCCTGGT TGCTGCCCTC 
AAATATTTCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413669.1
CDS	220..4194
Translation	MVLTRVGPSSSWTQSSSKGQTRGEAEPAKSFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557809.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557809.1

ORF Insert Sequence:

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGATAAA 
ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG GTAAACTTGG TATCTCCCAG GACGATAGTG ATATCACACA CATGGAACAT 
AATCTGGCAT CAAGAAGTTC ATCAGATGAT TTCCATGACT GTCAGACAAC CCCATCTTCA 
GCAGTTAAAA CACTTGAAGT AAATTCCACC AATAAAAATC CTTTCGATGG AGAGAAAATA 
ACTGTTAAAA TAGGACCTTG GACAGAGCTT CAACAAGATG AAATATTTGT AGATAATTTT 
CTATCCACTT TTGAATCCTT AGACTCTAAT GGTAAATCCA AGTCTATAGA AATAACACTT 
GAAAAGGAGG CTTTACAGGA AGCAAAATGT CATTCTTTTG GAGAATCATT AGCTAAATTA 
AGAAATAATC TACCTGCTCC TACAAAAGAA TATCATGTTG TAGTAAGTGG AGATACAATT 
AAATTACCAG ATACTAATGC CACATATGCC TCATCTAGAT TTTCAGATTC AGGTGTTGAA 
AGTGAACCAA GCTCTTTTGC AACACATCCA AACCTGGAAA TAGTCTCTGA AACTGGCCAA 
GGGCAAGGTC CTTACCATAA TGAAAGGTTA TTTCCTCAGC TTTTGATGAA ACCTGATTAC 
AATGTCAAGT TTTCATTAGG AAGTCACTGT ACTGAGAGTA CAAGTGCTTT AAGTGAAATA 
CAGTCATCTT TGACATCAAT AAACTCTCTA CCTTCTGATG ATGAACTCTC ACCTGATGAA 
AATTCTAAGA AATCTTCTGT GCCTGAATGC CATCTAAGTG ATAGTAAAAC TGTATTAAAT 
CTAGGAACAA TGGATTTGCC AAAGTGTGAT GATACTAAAA AGTCAAGTAT TATTTTGCAG 
CCTCAATGTG TTGTATTTTC AGGGCATTTG GACAATGAAA CTTTAGCAAT ACATTCCTTA 
AATTCAAGCA CTGAAGACCC TTTACAACAT GTTTTTTCAG ATAAAGATAC TTCTAGTGAT 
GTGAAAAGTA GTTGTAGCTC CCAGCCTAAT TTGGACCCTA TATTTAAAGG CTCCCAGAGT 
CATGATACAT CTAATAACAA CTCTGCAGGA ACCGTGATCG CAATACAGTC AGAACTGATT 
TGCTTAGGCA CTCCTTGTGT TGTTTCGGGT TCTGTTTCTA CTAATTCAGA ACTAAGTCAA 
GAGGGAACTG TGGAAAGAAA AAGTAGTGAC CCATTAAGTC TCAAACAAAT GTATTCAGAA 
GCCCCTTCAA TTGAACATGA ACTTCATCTG GGTTCGAGTG ACCCTCTTTC AGCCAGTACT 
GATATGGTAA AAGAAGGGCT TGTGGAAAAT TATTTTGGTT CTCAGAGCAG TACAGATATT 
TCGGACATAC GTGCTGTTAG CTACCACAAC TCAGTTAGCC CTCAGAAGGA AACCTGTGAA 
AAAGGAATCA GTGACCTTCA GCAGGAACAG GGCAAAGATG AGGAGGAGGA AGAACAGGAT 
CAACAAATGA TTCAAAATGG GTATTATGAA GAAACAGATT TTTCAGCTTT GGGTGGAACT 
ATAAATGCTC ACTTTCCCAG CAGAGATGTG CAAACAGAGG AAAGGCATCT AAAATCTGAA 
GAAGTGAACA GTGACTATCT GAGGGACAGT GTGAATGTGC CTACTGTTTG TACTTCGGGT 
TGTTTGTCTT TCCCTTCTGC TCCACGAGAG TCTCCTTGTA GTGTTAAATA CTCTTCTAGA 
AGTAAATTTG ATGCCATTAC AAAGCAGCCA AGCAGCATTT CTTACAACTT CACTTCTTCA 
ATTTCCTGGT ATGAAAACTC ACCAAAACCT CAAATACAAG CCTTCCTTCA GGCAAAAGAA 
GAACTGAAGC AACTTAAACT CCCTGGGTTC ATGTACAGTG ATGTTCCTCT GCTGGCATCC 
TCAGTACCTT ATTTCAGCAT GGAAGAAGAA GATGGTTCTG AAGATGGTGT CCATCTCATT 
GTCTGCGTGC ATGGTTTAGA TGGTAACAGT GCAGATCTCC GACTAGTAAA AACGTATATT 
GAACTTGGAC TGCCTGGGGG AAGAGTTGAT TTTCTTATGT CTGAGAGAAA TCAGAATGAT 
ACTTTCGCTG ATTTTGATAG CATGACTGAT CGTCTTTTGG ATGAAATCAT ACAGTATATT 
CAGATATATA GTTTAACAGT CTCAAAAATA AGCTTTATTG GACATTCATT GGGCAATTTA 
ATAATCCGTT CAGTGCTCAC AAGGCCACGG TTTAAATATT ACCTCAGCAA ACTTCATACC 
TTTCTCTCTC TTTCTGGGCC TCACCTTGGT ACACTGTACA ATAGCAGTGC TCTCGTCAAT 
ACAGGTCTCT GGTTCATGCA GAAATGGAAA AAATCAGGTT CTCTTTTGCA ACTGACATGT 
CGAGATCATT CAGACCCTCG CCAAACTTTT TTATATAAGC TTAGTAATAA AGCAGGGCTT 
CATTATTTCA AGAATGTTGT GCTGGTAGGA TCACTACAGG ATCGCTATGT TCCATATCAT 
TCTGCCCGCA TTGAAATGTG CAAAACAGCC TTGAAGGACA AACAGTCAGG ACAAATCTAT 
TCAGAAATGA TCCACAACTT GCTTCGCCCA GTTCTGCAAA GCAAAGACTG TAATTTGATT 
CGATACAATG TCATCAATGC ATTGCCCAAC ACGGCTGATT CACTCATCGG GAGAGCTGCA 
CACATAGCTG TTCTTGATTC GGAAATATTT TTAGAGAAGT TCTTCCTGGT TGCTGCCCTC 
AAATATTTCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_017795165.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650654.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X3, mRNA.

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CloneID	ONa38694
Clone ID Related Accession (Same CDS sequence)	XM_017795165.1 , XM_017795165.2
Accession Version	XM_017795165.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGGT AAACTTGGTA TCTCCCAGGA CGATAGTGAT 
ATCACACACA TGGAACATAA TCTGGCATCA AGAAGTTCAT CAGATGATTT CCATGACTGT 
CAGACAACCC CATCTTCAGC AGTTAAAACA CTTGAAGTAA ATTCCACCAA TAAAAATCCT 
TTCGATGGAG AGAAAATAAC TGTTAAAATA GGACCTTGGA CAGAGCTTCA ACAAGATGAA 
ATATTTGTAG ATAATTTTCT ATCCACTTTT GAATCCTTAG ACTCTAATGG TAAATCCAAG 
TCTATAGAAA TAACACTTGA AAAGGAGGCT TTACAGGAAG CAAAATGTCA TTCTTTTGGA 
GAATCATTAG CTAAATTAAG AAATAATCTA CCTGCTCCTA CAAAAGAATA TCATGTTGTA 
GTAAGTGGAG ATACAATTAA ATTACCAGAT ACTAATGCCA CATATGCCTC ATCTAGATTT 
TCAGATTCAG GTGTTGAAAG TGAACCAAGC TCTTTTGCAA CACATCCAAA CCTGGAAATA 
GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT TACCATAATG AAAGGTTATT TCCTCAGCTT 
TTGATGAAAC CTGATTACAA TGTCAAGTTT TCATTAGGAA GTCACTGTAC TGAGAGTACA 
AGTGCTTTAA GTGAAATACA GTCATCTTTG ACATCAATAA ACTCTCTACC TTCTGATGAT 
GAACTCTCAC CTGATGAAAA TTCTAAGAAA TCTTCTGTGC CTGAATGCCA TCTAAGTGAT 
AGTAAAACTG TATTAAATCT AGGAACAATG GATTTGCCAA AGTGTGATGA TACTAAAAAG 
TCAAGTATTA TTTTGCAGCC TCAATGTGTT GTATTTTCAG GGCATTTGGA CAATGAAACT 
TTAGCAATAC ATTCCTTAAA TTCAAGCACT GAAGACCCTT TACAACATGT TTTTTCAGAT 
AAAGATACTT CTAGTGATGT GAAAAGTAGT TGTAGCTCCC AGCCTAATTT GGACCCTATA 
TTTAAAGGCT CCCAGAGTCA TGATACATCT AATAACAACT CTGCAGGAAC CGTGATCGCA 
ATACAGTCAG AACTGATTTG CTTAGGCACT CCTTGTGTTG TTTCGGGTTC TGTTTCTACT 
AATTCAGAAC TAAGTCAAGA GGGAACTGTG GAAAGAAAAA GTAGTGACCC ATTAAGTCTC 
AAACAAATGT ATTCAGAAGC CCCTTCAATT GAACATGAAC TTCATCTGGG TTCGAGTGAC 
CCTCTTTCAG CCAGTACTGA TATGGTAAAA GAAGGGCTTG TGGAAAATTA TTTTGGTTCT 
CAGAGCAGTA CAGATATTTC GGACATACGT GCTGTTAGCT ACCACAACTC AGTTAGCCCT 
CAGAAGGAAA CCTGTGAAAA AGGAATCAGT GACCTTCAGC AGGAACAGGG CAAAGATGAG 
GAGGAGGAAG AACAGGATCA ACAAATGATT CAAAATGGGT ATTATGAAGA AACAGATTTT 
TCAGCTTTGG GTGGAACTAT AAATGCTCAC TTTCCCAGCA GAGATGTGCA AACAGAGGAA 
AGGCATCTAA AATCTGAAGA AGTGAACAGT GACTATCTGA GGGACAGTGT GAATGTGCCT 
ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC CCTTCTGCTC CACGAGAGTC TCCTTGTAGT 
GTTAAATACT CTTCTAGAAG TAAATTTGAT GCCATTACAA AGCAGCCAAG CAGCATTTCT 
TACAACTTCA CTTCTTCAAT TTCCTGGTAT GAAAACTCAC CAAAACCTCA AATACAAGCC 
TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA CTTAAACTCC CTGGGTTCAT GTACAGTGAT 
GTTCCTCTGC TGGCATCCTC AGTACCTTAT TTCAGCATGG AAGAAGAAGA TGGTTCTGAA 
GATGGTGTCC ATCTCATTGT CTGCGTGCAT GGTTTAGATG GTAACAGTGC AGATCTCCGA 
CTAGTAAAAA CGTATATTGA ACTTGGACTG CCTGGGGGAA GAGTTGATTT TCTTATGTCT 
GAGAGAAATC AGAATGATAC TTTCGCTGAT TTTGATAGCA TGACTGATCG TCTTTTGGAT 
GAAATCATAC AGTATATTCA GATATATAGT TTAACAGTCT CAAAAATAAG CTTTATTGGA 
CATTCATTGG GCAATTTAAT AATCCGTTCA GTGCTCACAA GGCCACGGTT TAAATATTAC 
CTCAGCAAAC TTCATACCTT TCTCTCTCTT TCTGGGCCTC ACCTTGGTAC ACTGTACAAT 
AGCAGTGCTC TCGTCAATAC AGGTCTCTGG TTCATGCAGA AATGGAAAAA ATCAGGTTCT 
CTTTTGCAAC TGACATGTCG AGATCATTCA GACCCTCGCC AAACTTTTTT ATATAAGCTT 
AGTAATAAAG CAGGGCTTCA TTATTTCAAG AATGTTGTGC TGGTAGGATC ACTACAGGAT 
CGCTATGTTC CATATCATTC TGCCCGCATT GAAATGTGCA AAACAGCCTT GAAGGACAAA 
CAGTCAGGAC AAATCTATTC AGAAATGATC CACAACTTGC TTCGCCCAGT TCTGCAAAGC 
AAAGACTGTA ATTTGATTCG ATACAATGTC ATCAATGCAT TGCCCAACAC GGCTGATTCA 
CTCATCGGGA GAGCTGCACA CATAGCTGTT CTTGATTCGG AAATATTTTT AGAGAAGTTC 
TTCCTGGTTG CTGCCCTCAA ATATTTCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650654.1
CDS	108..4160
Translation	MVLTRVGPSSSWTQSSSKGQTRGEAEPAKSFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_017795165.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795165.1

ORF Insert Sequence:

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGGT AAACTTGGTA TCTCCCAGGA CGATAGTGAT 
ATCACACACA TGGAACATAA TCTGGCATCA AGAAGTTCAT CAGATGATTT CCATGACTGT 
CAGACAACCC CATCTTCAGC AGTTAAAACA CTTGAAGTAA ATTCCACCAA TAAAAATCCT 
TTCGATGGAG AGAAAATAAC TGTTAAAATA GGACCTTGGA CAGAGCTTCA ACAAGATGAA 
ATATTTGTAG ATAATTTTCT ATCCACTTTT GAATCCTTAG ACTCTAATGG TAAATCCAAG 
TCTATAGAAA TAACACTTGA AAAGGAGGCT TTACAGGAAG CAAAATGTCA TTCTTTTGGA 
GAATCATTAG CTAAATTAAG AAATAATCTA CCTGCTCCTA CAAAAGAATA TCATGTTGTA 
GTAAGTGGAG ATACAATTAA ATTACCAGAT ACTAATGCCA CATATGCCTC ATCTAGATTT 
TCAGATTCAG GTGTTGAAAG TGAACCAAGC TCTTTTGCAA CACATCCAAA CCTGGAAATA 
GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT TACCATAATG AAAGGTTATT TCCTCAGCTT 
TTGATGAAAC CTGATTACAA TGTCAAGTTT TCATTAGGAA GTCACTGTAC TGAGAGTACA 
AGTGCTTTAA GTGAAATACA GTCATCTTTG ACATCAATAA ACTCTCTACC TTCTGATGAT 
GAACTCTCAC CTGATGAAAA TTCTAAGAAA TCTTCTGTGC CTGAATGCCA TCTAAGTGAT 
AGTAAAACTG TATTAAATCT AGGAACAATG GATTTGCCAA AGTGTGATGA TACTAAAAAG 
TCAAGTATTA TTTTGCAGCC TCAATGTGTT GTATTTTCAG GGCATTTGGA CAATGAAACT 
TTAGCAATAC ATTCCTTAAA TTCAAGCACT GAAGACCCTT TACAACATGT TTTTTCAGAT 
AAAGATACTT CTAGTGATGT GAAAAGTAGT TGTAGCTCCC AGCCTAATTT GGACCCTATA 
TTTAAAGGCT CCCAGAGTCA TGATACATCT AATAACAACT CTGCAGGAAC CGTGATCGCA 
ATACAGTCAG AACTGATTTG CTTAGGCACT CCTTGTGTTG TTTCGGGTTC TGTTTCTACT 
AATTCAGAAC TAAGTCAAGA GGGAACTGTG GAAAGAAAAA GTAGTGACCC ATTAAGTCTC 
AAACAAATGT ATTCAGAAGC CCCTTCAATT GAACATGAAC TTCATCTGGG TTCGAGTGAC 
CCTCTTTCAG CCAGTACTGA TATGGTAAAA GAAGGGCTTG TGGAAAATTA TTTTGGTTCT 
CAGAGCAGTA CAGATATTTC GGACATACGT GCTGTTAGCT ACCACAACTC AGTTAGCCCT 
CAGAAGGAAA CCTGTGAAAA AGGAATCAGT GACCTTCAGC AGGAACAGGG CAAAGATGAG 
GAGGAGGAAG AACAGGATCA ACAAATGATT CAAAATGGGT ATTATGAAGA AACAGATTTT 
TCAGCTTTGG GTGGAACTAT AAATGCTCAC TTTCCCAGCA GAGATGTGCA AACAGAGGAA 
AGGCATCTAA AATCTGAAGA AGTGAACAGT GACTATCTGA GGGACAGTGT GAATGTGCCT 
ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC CCTTCTGCTC CACGAGAGTC TCCTTGTAGT 
GTTAAATACT CTTCTAGAAG TAAATTTGAT GCCATTACAA AGCAGCCAAG CAGCATTTCT 
TACAACTTCA CTTCTTCAAT TTCCTGGTAT GAAAACTCAC CAAAACCTCA AATACAAGCC 
TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA CTTAAACTCC CTGGGTTCAT GTACAGTGAT 
GTTCCTCTGC TGGCATCCTC AGTACCTTAT TTCAGCATGG AAGAAGAAGA TGGTTCTGAA 
GATGGTGTCC ATCTCATTGT CTGCGTGCAT GGTTTAGATG GTAACAGTGC AGATCTCCGA 
CTAGTAAAAA CGTATATTGA ACTTGGACTG CCTGGGGGAA GAGTTGATTT TCTTATGTCT 
GAGAGAAATC AGAATGATAC TTTCGCTGAT TTTGATAGCA TGACTGATCG TCTTTTGGAT 
GAAATCATAC AGTATATTCA GATATATAGT TTAACAGTCT CAAAAATAAG CTTTATTGGA 
CATTCATTGG GCAATTTAAT AATCCGTTCA GTGCTCACAA GGCCACGGTT TAAATATTAC 
CTCAGCAAAC TTCATACCTT TCTCTCTCTT TCTGGGCCTC ACCTTGGTAC ACTGTACAAT 
AGCAGTGCTC TCGTCAATAC AGGTCTCTGG TTCATGCAGA AATGGAAAAA ATCAGGTTCT 
CTTTTGCAAC TGACATGTCG AGATCATTCA GACCCTCGCC AAACTTTTTT ATATAAGCTT 
AGTAATAAAG CAGGGCTTCA TTATTTCAAG AATGTTGTGC TGGTAGGATC ACTACAGGAT 
CGCTATGTTC CATATCATTC TGCCCGCATT GAAATGTGCA AAACAGCCTT GAAGGACAAA 
CAGTCAGGAC AAATCTATTC AGAAATGATC CACAACTTGC TTCGCCCAGT TCTGCAAAGC 
AAAGACTGTA ATTTGATTCG ATACAATGTC ATCAATGCAT TGCCCAACAC GGCTGATTCA 
CTCATCGGGA GAGCTGCACA CATAGCTGTT CTTGATTCGG AAATATTTTT AGAGAAGTTC 
TTCCTGGTTG CTGCCCTCAA ATATTTCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_017795165.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650654.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X8, mRNA.

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CloneID	ONa38694
Clone ID Related Accession (Same CDS sequence)	XM_017795165.1 , XM_017795165.2
Accession Version	XM_017795165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_017795165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGGT AAACTTGGTA TCTCCCAGGA CGATAGTGAT 
ATCACACACA TGGAACATAA TCTGGCATCA AGAAGTTCAT CAGATGATTT CCATGACTGT 
CAGACAACCC CATCTTCAGC AGTTAAAACA CTTGAAGTAA ATTCCACCAA TAAAAATCCT 
TTCGATGGAG AGAAAATAAC TGTTAAAATA GGACCTTGGA CAGAGCTTCA ACAAGATGAA 
ATATTTGTAG ATAATTTTCT ATCCACTTTT GAATCCTTAG ACTCTAATGG TAAATCCAAG 
TCTATAGAAA TAACACTTGA AAAGGAGGCT TTACAGGAAG CAAAATGTCA TTCTTTTGGA 
GAATCATTAG CTAAATTAAG AAATAATCTA CCTGCTCCTA CAAAAGAATA TCATGTTGTA 
GTAAGTGGAG ATACAATTAA ATTACCAGAT ACTAATGCCA CATATGCCTC ATCTAGATTT 
TCAGATTCAG GTGTTGAAAG TGAACCAAGC TCTTTTGCAA CACATCCAAA CCTGGAAATA 
GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT TACCATAATG AAAGGTTATT TCCTCAGCTT 
TTGATGAAAC CTGATTACAA TGTCAAGTTT TCATTAGGAA GTCACTGTAC TGAGAGTACA 
AGTGCTTTAA GTGAAATACA GTCATCTTTG ACATCAATAA ACTCTCTACC TTCTGATGAT 
GAACTCTCAC CTGATGAAAA TTCTAAGAAA TCTTCTGTGC CTGAATGCCA TCTAAGTGAT 
AGTAAAACTG TATTAAATCT AGGAACAATG GATTTGCCAA AGTGTGATGA TACTAAAAAG 
TCAAGTATTA TTTTGCAGCC TCAATGTGTT GTATTTTCAG GGCATTTGGA CAATGAAACT 
TTAGCAATAC ATTCCTTAAA TTCAAGCACT GAAGACCCTT TACAACATGT TTTTTCAGAT 
AAAGATACTT CTAGTGATGT GAAAAGTAGT TGTAGCTCCC AGCCTAATTT GGACCCTATA 
TTTAAAGGCT CCCAGAGTCA TGATACATCT AATAACAACT CTGCAGGAAC CGTGATCGCA 
ATACAGTCAG AACTGATTTG CTTAGGCACT CCTTGTGTTG TTTCGGGTTC TGTTTCTACT 
AATTCAGAAC TAAGTCAAGA GGGAACTGTG GAAAGAAAAA GTAGTGACCC ATTAAGTCTC 
AAACAAATGT ATTCAGAAGC CCCTTCAATT GAACATGAAC TTCATCTGGG TTCGAGTGAC 
CCTCTTTCAG CCAGTACTGA TATGGTAAAA GAAGGGCTTG TGGAAAATTA TTTTGGTTCT 
CAGAGCAGTA CAGATATTTC GGACATACGT GCTGTTAGCT ACCACAACTC AGTTAGCCCT 
CAGAAGGAAA CCTGTGAAAA AGGAATCAGT GACCTTCAGC AGGAACAGGG CAAAGATGAG 
GAGGAGGAAG AACAGGATCA ACAAATGATT CAAAATGGGT ATTATGAAGA AACAGATTTT 
TCAGCTTTGG GTGGAACTAT AAATGCTCAC TTTCCCAGCA GAGATGTGCA AACAGAGGAA 
AGGCATCTAA AATCTGAAGA AGTGAACAGT GACTATCTGA GGGACAGTGT GAATGTGCCT 
ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC CCTTCTGCTC CACGAGAGTC TCCTTGTAGT 
GTTAAATACT CTTCTAGAAG TAAATTTGAT GCCATTACAA AGCAGCCAAG CAGCATTTCT 
TACAACTTCA CTTCTTCAAT TTCCTGGTAT GAAAACTCAC CAAAACCTCA AATACAAGCC 
TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA CTTAAACTCC CTGGGTTCAT GTACAGTGAT 
GTTCCTCTGC TGGCATCCTC AGTACCTTAT TTCAGCATGG AAGAAGAAGA TGGTTCTGAA 
GATGGTGTCC ATCTCATTGT CTGCGTGCAT GGTTTAGATG GTAACAGTGC AGATCTCCGA 
CTAGTAAAAA CGTATATTGA ACTTGGACTG CCTGGGGGAA GAGTTGATTT TCTTATGTCT 
GAGAGAAATC AGAATGATAC TTTCGCTGAT TTTGATAGCA TGACTGATCG TCTTTTGGAT 
GAAATCATAC AGTATATTCA GATATATAGT TTAACAGTCT CAAAAATAAG CTTTATTGGA 
CATTCATTGG GCAATTTAAT AATCCGTTCA GTGCTCACAA GGCCACGGTT TAAATATTAC 
CTCAGCAAAC TTCATACCTT TCTCTCTCTT TCTGGGCCTC ACCTTGGTAC ACTGTACAAT 
AGCAGTGCTC TCGTCAATAC AGGTCTCTGG TTCATGCAGA AATGGAAAAA ATCAGGTTCT 
CTTTTGCAAC TGACATGTCG AGATCATTCA GACCCTCGCC AAACTTTTTT ATATAAGCTT 
AGTAATAAAG CAGGGCTTCA TTATTTCAAG AATGTTGTGC TGGTAGGATC ACTACAGGAT 
CGCTATGTTC CATATCATTC TGCCCGCATT GAAATGTGCA AAACAGCCTT GAAGGACAAA 
CAGTCAGGAC AAATCTATTC AGAAATGATC CACAACTTGC TTCGCCCAGT TCTGCAAAGC 
AAAGACTGTA ATTTGATTCG ATACAATGTC ATCAATGCAT TGCCCAACAC GGCTGATTCA 
CTCATCGGGA GAGCTGCACA CATAGCTGTT CTTGATTCGG AAATATTTTT AGAGAAGTTC 
TTCCTGGTTG CTGCCCTCAA ATATTTCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650654.1
CDS	220..4272
Translation	MVLTRVGPSSSWTQSSSKGQTRGEAEPAKSFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_017795165.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795165.2

ORF Insert Sequence:

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGGT AAACTTGGTA TCTCCCAGGA CGATAGTGAT 
ATCACACACA TGGAACATAA TCTGGCATCA AGAAGTTCAT CAGATGATTT CCATGACTGT 
CAGACAACCC CATCTTCAGC AGTTAAAACA CTTGAAGTAA ATTCCACCAA TAAAAATCCT 
TTCGATGGAG AGAAAATAAC TGTTAAAATA GGACCTTGGA CAGAGCTTCA ACAAGATGAA 
ATATTTGTAG ATAATTTTCT ATCCACTTTT GAATCCTTAG ACTCTAATGG TAAATCCAAG 
TCTATAGAAA TAACACTTGA AAAGGAGGCT TTACAGGAAG CAAAATGTCA TTCTTTTGGA 
GAATCATTAG CTAAATTAAG AAATAATCTA CCTGCTCCTA CAAAAGAATA TCATGTTGTA 
GTAAGTGGAG ATACAATTAA ATTACCAGAT ACTAATGCCA CATATGCCTC ATCTAGATTT 
TCAGATTCAG GTGTTGAAAG TGAACCAAGC TCTTTTGCAA CACATCCAAA CCTGGAAATA 
GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT TACCATAATG AAAGGTTATT TCCTCAGCTT 
TTGATGAAAC CTGATTACAA TGTCAAGTTT TCATTAGGAA GTCACTGTAC TGAGAGTACA 
AGTGCTTTAA GTGAAATACA GTCATCTTTG ACATCAATAA ACTCTCTACC TTCTGATGAT 
GAACTCTCAC CTGATGAAAA TTCTAAGAAA TCTTCTGTGC CTGAATGCCA TCTAAGTGAT 
AGTAAAACTG TATTAAATCT AGGAACAATG GATTTGCCAA AGTGTGATGA TACTAAAAAG 
TCAAGTATTA TTTTGCAGCC TCAATGTGTT GTATTTTCAG GGCATTTGGA CAATGAAACT 
TTAGCAATAC ATTCCTTAAA TTCAAGCACT GAAGACCCTT TACAACATGT TTTTTCAGAT 
AAAGATACTT CTAGTGATGT GAAAAGTAGT TGTAGCTCCC AGCCTAATTT GGACCCTATA 
TTTAAAGGCT CCCAGAGTCA TGATACATCT AATAACAACT CTGCAGGAAC CGTGATCGCA 
ATACAGTCAG AACTGATTTG CTTAGGCACT CCTTGTGTTG TTTCGGGTTC TGTTTCTACT 
AATTCAGAAC TAAGTCAAGA GGGAACTGTG GAAAGAAAAA GTAGTGACCC ATTAAGTCTC 
AAACAAATGT ATTCAGAAGC CCCTTCAATT GAACATGAAC TTCATCTGGG TTCGAGTGAC 
CCTCTTTCAG CCAGTACTGA TATGGTAAAA GAAGGGCTTG TGGAAAATTA TTTTGGTTCT 
CAGAGCAGTA CAGATATTTC GGACATACGT GCTGTTAGCT ACCACAACTC AGTTAGCCCT 
CAGAAGGAAA CCTGTGAAAA AGGAATCAGT GACCTTCAGC AGGAACAGGG CAAAGATGAG 
GAGGAGGAAG AACAGGATCA ACAAATGATT CAAAATGGGT ATTATGAAGA AACAGATTTT 
TCAGCTTTGG GTGGAACTAT AAATGCTCAC TTTCCCAGCA GAGATGTGCA AACAGAGGAA 
AGGCATCTAA AATCTGAAGA AGTGAACAGT GACTATCTGA GGGACAGTGT GAATGTGCCT 
ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC CCTTCTGCTC CACGAGAGTC TCCTTGTAGT 
GTTAAATACT CTTCTAGAAG TAAATTTGAT GCCATTACAA AGCAGCCAAG CAGCATTTCT 
TACAACTTCA CTTCTTCAAT TTCCTGGTAT GAAAACTCAC CAAAACCTCA AATACAAGCC 
TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA CTTAAACTCC CTGGGTTCAT GTACAGTGAT 
GTTCCTCTGC TGGCATCCTC AGTACCTTAT TTCAGCATGG AAGAAGAAGA TGGTTCTGAA 
GATGGTGTCC ATCTCATTGT CTGCGTGCAT GGTTTAGATG GTAACAGTGC AGATCTCCGA 
CTAGTAAAAA CGTATATTGA ACTTGGACTG CCTGGGGGAA GAGTTGATTT TCTTATGTCT 
GAGAGAAATC AGAATGATAC TTTCGCTGAT TTTGATAGCA TGACTGATCG TCTTTTGGAT 
GAAATCATAC AGTATATTCA GATATATAGT TTAACAGTCT CAAAAATAAG CTTTATTGGA 
CATTCATTGG GCAATTTAAT AATCCGTTCA GTGCTCACAA GGCCACGGTT TAAATATTAC 
CTCAGCAAAC TTCATACCTT TCTCTCTCTT TCTGGGCCTC ACCTTGGTAC ACTGTACAAT 
AGCAGTGCTC TCGTCAATAC AGGTCTCTGG TTCATGCAGA AATGGAAAAA ATCAGGTTCT 
CTTTTGCAAC TGACATGTCG AGATCATTCA GACCCTCGCC AAACTTTTTT ATATAAGCTT 
AGTAATAAAG CAGGGCTTCA TTATTTCAAG AATGTTGTGC TGGTAGGATC ACTACAGGAT 
CGCTATGTTC CATATCATTC TGCCCGCATT GAAATGTGCA AAACAGCCTT GAAGGACAAA 
CAGTCAGGAC AAATCTATTC AGAAATGATC CACAACTTGC TTCGCCCAGT TCTGCAAAGC 
AAAGACTGTA ATTTGATTCG ATACAATGTC ATCAATGCAT TGCCCAACAC GGCTGATTCA 
CTCATCGGGA GAGCTGCACA CATAGCTGTT CTTGATTCGG AAATATTTTT AGAGAAGTTC 
TTCCTGGTTG CTGCCCTCAA ATATTTCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_008856285.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008854507.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ONa38695
Clone ID Related Accession (Same CDS sequence)	XM_008856285.1 , XM_008856285.2 , XM_029557811.1
Accession Version	XM_008856285.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008854507.1
CDS	516..4478
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_008856285.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008856285.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_008856285.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008854507.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ONa38695
Clone ID Related Accession (Same CDS sequence)	XM_008856285.1 , XM_008856285.2 , XM_029557811.1
Accession Version	XM_008856285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008856285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008854507.1
CDS	525..4487
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_008856285.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008856285.2

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_029557811.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413671.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X12, mRNA.

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CloneID	ONa38695
Clone ID Related Accession (Same CDS sequence)	XM_008856285.1 , XM_008856285.2 , XM_029557811.1
Accession Version	XM_029557811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413671.1
CDS	437..4399
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557811.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557811.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_017795166.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650655.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ONa38696
Clone ID Related Accession (Same CDS sequence)	XM_017795166.1
Accession Version	XM_017795166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG ACTTAGATGC ACCCTGGACG GGAATTCAGA CTCTTCAGAT ATCAGAGTCC 
AGTAGAATGG ATAAATATGA GACTGAAGAA AGCTCTGTAG CAGGACTTTC TAGCCCAGAG 
TTGAAAGTCA GAACTGCTGG TGCCTCCAGT ATATGCTGTA CAGATGGTGA AAAGCAGCTA 
ACAAAGTCTC AAAAAGGAAA GAATGAAGAA TTAAATAAAT CCAAAGTTAA GGTCACTAAG 
CTCATGAAAA CAATGAAACA AGAGAACACA AAAAAGTTAA TAAAACAGAA CTCTAAAGAT 
TCTGTGATTT TAGTAAGCTA CAGATGTTTG AAAAGTACAC CATCAAGTGA TCTTAGTAAA 
TGCTTTGAAG GCAATCCTGC ACATAGTCAG AAAGAAGGTC TGGATCCCAC AATATGTGGA 
TATAATTTTG ACCCAAAGAC CTACATCAGA CAGACAAGTC AAAAGGAAGC TAGCTCTTTG 
CCAGCTAATA CAGAGAGAAC TGAACAAAAG TCTCCAGATG TTGAAAATGT GCAACCAGAC 
CAGTTTGATC CCTTGAACTC TGGCAGCCTA AATCTTTGTG CGCATTTGTC CATTTCAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650655.1
CDS	58..3780
Translation	MDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_017795166.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795166.1

ORF Insert Sequence:

ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG ACTTAGATGC ACCCTGGACG GGAATTCAGA CTCTTCAGAT ATCAGAGTCC 
AGTAGAATGG ATAAATATGA GACTGAAGAA AGCTCTGTAG CAGGACTTTC TAGCCCAGAG 
TTGAAAGTCA GAACTGCTGG TGCCTCCAGT ATATGCTGTA CAGATGGTGA AAAGCAGCTA 
ACAAAGTCTC AAAAAGGAAA GAATGAAGAA TTAAATAAAT CCAAAGTTAA GGTCACTAAG 
CTCATGAAAA CAATGAAACA AGAGAACACA AAAAAGTTAA TAAAACAGAA CTCTAAAGAT 
TCTGTGATTT TAGTAAGCTA CAGATGTTTG AAAAGTACAC CATCAAGTGA TCTTAGTAAA 
TGCTTTGAAG GCAATCCTGC ACATAGTCAG AAAGAAGGTC TGGATCCCAC AATATGTGGA 
TATAATTTTG ACCCAAAGAC CTACATCAGA CAGACAAGTC AAAAGGAAGC TAGCTCTTTG 
CCAGCTAATA CAGAGAGAAC TGAACAAAAG TCTCCAGATG TTGAAAATGT GCAACCAGAC 
CAGTTTGATC CCTTGAACTC TGGCAGCCTA AATCTTTGTG CGCATTTGTC CATTTCAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_029557803.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413663.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa138118
Clone ID Related Accession (Same CDS sequence)	XM_029557803.1 , XM_029557804.1 , XM_029557805.1
Accession Version	XM_029557803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGACTT AGATGCACCC TGGACGGGAA TTCAGACTCT TCAGATATCA 
GAGTCCAGTA GAATGGATAA ATATGAGACT GAAGAAAGCT CTGTAGCAGG ACTTTCTAGC 
CCAGAGTTGA AAGTCAGAAC TGCTGGTGCC TCCAGTATAT GCTGTACAGA TGGTGAAAAG 
CAGCTAACAA AGTCTCAAAA AGGAAAGAAT GAAGAATTAA ATAAATCCAA AGTTAAGGTC 
ACTAAGCTCA TGAAAACAAT GAAACAAGAG AACACAAAAA AGTTAATAAA ACAGAACTCT 
AAAGATTCTG TGATTTTAGT AAGCTACAGA TGTTTGAAAA GTACACCATC AAGTGATCTT 
AGTAAATGCT TTGAAGGCAA TCCTGCACAT AGTCAGAAAG AAGGTCTGGA TCCCACAATA 
TGTGGATATA ATTTTGACCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TCTTTGCCAG CTAATACAGA GAGAACTGAA CAAAAGTCTC CAGATGTTGA AAATGTGCAA 
CCAGACCAGT TTGATCCCTT GAACTCTGGC AGCCTAAATC TTTGTGCGCA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGACGAT AGTGATATCA CACACATGGA ACATAATCTG 
GCATCAAGAA GTTCATCAGA TGATTTCCAT GACTGTCAGA CAACCCCATC TTCAGCAGTT 
AAAACACTTG AAGTAAATTC CACCAATAAA AATCCTTTCG ATGGAGAGAA AATAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTAGATAA TTTTCTATCC 
ACTTTTGAAT CCTTAGACTC TAATGGTAAA TCCAAGTCTA TAGAAATAAC ACTTGAAAAG 
GAGGCTTTAC AGGAAGCAAA ATGTCATTCT TTTGGAGAAT CATTAGCTAA ATTAAGAAAT 
AATCTACCTG CTCCTACAAA AGAATATCAT GTTGTAGTAA GTGGAGATAC AATTAAATTA 
CCAGATACTA ATGCCACATA TGCCTCATCT AGATTTTCAG ATTCAGGTGT TGAAAGTGAA 
CCAAGCTCTT TTGCAACACA TCCAAACCTG GAAATAGTCT CTGAAACTGG CCAAGGGCAA 
GGTCCTTACC ATAATGAAAG GTTATTTCCT CAGCTTTTGA TGAAACCTGA TTACAATGTC 
AAGTTTTCAT TAGGAAGTCA CTGTACTGAG AGTACAAGTG CTTTAAGTGA AATACAGTCA 
TCTTTGACAT CAATAAACTC TCTACCTTCT GATGATGAAC TCTCACCTGA TGAAAATTCT 
AAGAAATCTT CTGTGCCTGA ATGCCATCTA AGTGATAGTA AAACTGTATT AAATCTAGGA 
ACAATGGATT TGCCAAAGTG TGATGATACT AAAAAGTCAA GTATTATTTT GCAGCCTCAA 
TGTGTTGTAT TTTCAGGGCA TTTGGACAAT GAAACTTTAG CAATACATTC CTTAAATTCA 
AGCACTGAAG ACCCTTTACA ACATGTTTTT TCAGATAAAG ATACTTCTAG TGATGTGAAA 
AGTAGTTGTA GCTCCCAGCC TAATTTGGAC CCTATATTTA AAGGCTCCCA GAGTCATGAT 
ACATCTAATA ACAACTCTGC AGGAACCGTG ATCGCAATAC AGTCAGAACT GATTTGCTTA 
GGCACTCCTT GTGTTGTTTC GGGTTCTGTT TCTACTAATT CAGAACTAAG TCAAGAGGGA 
ACTGTGGAAA GAAAAAGTAG TGACCCATTA AGTCTCAAAC AAATGTATTC AGAAGCCCCT 
TCAATTGAAC ATGAACTTCA TCTGGGTTCG AGTGACCCTC TTTCAGCCAG TACTGATATG 
GTAAAAGAAG GGCTTGTGGA AAATTATTTT GGTTCTCAGA GCAGTACAGA TATTTCGGAC 
ATACGTGCTG TTAGCTACCA CAACTCAGTT AGCCCTCAGA AGGAAACCTG TGAAAAAGGA 
ATCAGTGACC TTCAGCAGGA ACAGGGCAAA GATGAGGAGG AGGAAGAACA GGATCAACAA 
ATGATTCAAA ATGGGTATTA TGAAGAAACA GATTTTTCAG CTTTGGGTGG AACTATAAAT 
GCTCACTTTC CCAGCAGAGA TGTGCAAACA GAGGAAAGGC ATCTAAAATC TGAAGAAGTG 
AACAGTGACT ATCTGAGGGA CAGTGTGAAT GTGCCTACTG TTTGTACTTC GGGTTGTTTG 
TCTTTCCCTT CTGCTCCACG AGAGTCTCCT TGTAGTGTTA AATACTCTTC TAGAAGTAAA 
TTTGATGCCA TTACAAAGCA GCCAAGCAGC ATTTCTTACA ACTTCACTTC TTCAATTTCC 
TGGTATGAAA ACTCACCAAA ACCTCAAATA CAAGCCTTCC TTCAGGCAAA AGAAGAACTG 
AAGCAACTTA AACTCCCTGG GTTCATGTAC AGTGATGTTC CTCTGCTGGC ATCCTCAGTA 
CCTTATTTCA GCATGGAAGA AGAAGATGGT TCTGAAGATG GTGTCCATCT CATTGTCTGC 
GTGCATGGTT TAGATGGTAA CAGTGCAGAT CTCCGACTAG TAAAAACGTA TATTGAACTT 
GGACTGCCTG GGGGAAGAGT TGATTTTCTT ATGTCTGAGA GAAATCAGAA TGATACTTTC 
GCTGATTTTG ATAGCATGAC TGATCGTCTT TTGGATGAAA TCATACAGTA TATTCAGATA 
TATAGTTTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CATTGGGCAA TTTAATAATC 
CGTTCAGTGC TCACAAGGCC ACGGTTTAAA TATTACCTCA GCAAACTTCA TACCTTTCTC 
TCTCTTTCTG GGCCTCACCT TGGTACACTG TACAATAGCA GTGCTCTCGT CAATACAGGT 
CTCTGGTTCA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TGCAACTGAC ATGTCGAGAT 
CATTCAGACC CTCGCCAAAC TTTTTTATAT AAGCTTAGTA ATAAAGCAGG GCTTCATTAT 
TTCAAGAATG TTGTGCTGGT AGGATCACTA CAGGATCGCT ATGTTCCATA TCATTCTGCC 
CGCATTGAAA TGTGCAAAAC AGCCTTGAAG GACAAACAGT CAGGACAAAT CTATTCAGAA 
ATGATCCACA ACTTGCTTCG CCCAGTTCTG CAAAGCAAAG ACTGTAATTT GATTCGATAC 
AATGTCATCA ATGCATTGCC CAACACGGCT GATTCACTCA TCGGGAGAGC TGCACACATA 
GCTGTTCTTG ATTCGGAAAT ATTTTTAGAG AAGTTCTTCC TGGTTGCTGC CCTCAAATAT 
TTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413663.1
CDS	553..5181
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557803.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557803.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGACTT AGATGCACCC TGGACGGGAA TTCAGACTCT TCAGATATCA 
GAGTCCAGTA GAATGGATAA ATATGAGACT GAAGAAAGCT CTGTAGCAGG ACTTTCTAGC 
CCAGAGTTGA AAGTCAGAAC TGCTGGTGCC TCCAGTATAT GCTGTACAGA TGGTGAAAAG 
CAGCTAACAA AGTCTCAAAA AGGAAAGAAT GAAGAATTAA ATAAATCCAA AGTTAAGGTC 
ACTAAGCTCA TGAAAACAAT GAAACAAGAG AACACAAAAA AGTTAATAAA ACAGAACTCT 
AAAGATTCTG TGATTTTAGT AAGCTACAGA TGTTTGAAAA GTACACCATC AAGTGATCTT 
AGTAAATGCT TTGAAGGCAA TCCTGCACAT AGTCAGAAAG AAGGTCTGGA TCCCACAATA 
TGTGGATATA ATTTTGACCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TCTTTGCCAG CTAATACAGA GAGAACTGAA CAAAAGTCTC CAGATGTTGA AAATGTGCAA 
CCAGACCAGT TTGATCCCTT GAACTCTGGC AGCCTAAATC TTTGTGCGCA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGACGAT AGTGATATCA CACACATGGA ACATAATCTG 
GCATCAAGAA GTTCATCAGA TGATTTCCAT GACTGTCAGA CAACCCCATC TTCAGCAGTT 
AAAACACTTG AAGTAAATTC CACCAATAAA AATCCTTTCG ATGGAGAGAA AATAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTAGATAA TTTTCTATCC 
ACTTTTGAAT CCTTAGACTC TAATGGTAAA TCCAAGTCTA TAGAAATAAC ACTTGAAAAG 
GAGGCTTTAC AGGAAGCAAA ATGTCATTCT TTTGGAGAAT CATTAGCTAA ATTAAGAAAT 
AATCTACCTG CTCCTACAAA AGAATATCAT GTTGTAGTAA GTGGAGATAC AATTAAATTA 
CCAGATACTA ATGCCACATA TGCCTCATCT AGATTTTCAG ATTCAGGTGT TGAAAGTGAA 
CCAAGCTCTT TTGCAACACA TCCAAACCTG GAAATAGTCT CTGAAACTGG CCAAGGGCAA 
GGTCCTTACC ATAATGAAAG GTTATTTCCT CAGCTTTTGA TGAAACCTGA TTACAATGTC 
AAGTTTTCAT TAGGAAGTCA CTGTACTGAG AGTACAAGTG CTTTAAGTGA AATACAGTCA 
TCTTTGACAT CAATAAACTC TCTACCTTCT GATGATGAAC TCTCACCTGA TGAAAATTCT 
AAGAAATCTT CTGTGCCTGA ATGCCATCTA AGTGATAGTA AAACTGTATT AAATCTAGGA 
ACAATGGATT TGCCAAAGTG TGATGATACT AAAAAGTCAA GTATTATTTT GCAGCCTCAA 
TGTGTTGTAT TTTCAGGGCA TTTGGACAAT GAAACTTTAG CAATACATTC CTTAAATTCA 
AGCACTGAAG ACCCTTTACA ACATGTTTTT TCAGATAAAG ATACTTCTAG TGATGTGAAA 
AGTAGTTGTA GCTCCCAGCC TAATTTGGAC CCTATATTTA AAGGCTCCCA GAGTCATGAT 
ACATCTAATA ACAACTCTGC AGGAACCGTG ATCGCAATAC AGTCAGAACT GATTTGCTTA 
GGCACTCCTT GTGTTGTTTC GGGTTCTGTT TCTACTAATT CAGAACTAAG TCAAGAGGGA 
ACTGTGGAAA GAAAAAGTAG TGACCCATTA AGTCTCAAAC AAATGTATTC AGAAGCCCCT 
TCAATTGAAC ATGAACTTCA TCTGGGTTCG AGTGACCCTC TTTCAGCCAG TACTGATATG 
GTAAAAGAAG GGCTTGTGGA AAATTATTTT GGTTCTCAGA GCAGTACAGA TATTTCGGAC 
ATACGTGCTG TTAGCTACCA CAACTCAGTT AGCCCTCAGA AGGAAACCTG TGAAAAAGGA 
ATCAGTGACC TTCAGCAGGA ACAGGGCAAA GATGAGGAGG AGGAAGAACA GGATCAACAA 
ATGATTCAAA ATGGGTATTA TGAAGAAACA GATTTTTCAG CTTTGGGTGG AACTATAAAT 
GCTCACTTTC CCAGCAGAGA TGTGCAAACA GAGGAAAGGC ATCTAAAATC TGAAGAAGTG 
AACAGTGACT ATCTGAGGGA CAGTGTGAAT GTGCCTACTG TTTGTACTTC GGGTTGTTTG 
TCTTTCCCTT CTGCTCCACG AGAGTCTCCT TGTAGTGTTA AATACTCTTC TAGAAGTAAA 
TTTGATGCCA TTACAAAGCA GCCAAGCAGC ATTTCTTACA ACTTCACTTC TTCAATTTCC 
TGGTATGAAA ACTCACCAAA ACCTCAAATA CAAGCCTTCC TTCAGGCAAA AGAAGAACTG 
AAGCAACTTA AACTCCCTGG GTTCATGTAC AGTGATGTTC CTCTGCTGGC ATCCTCAGTA 
CCTTATTTCA GCATGGAAGA AGAAGATGGT TCTGAAGATG GTGTCCATCT CATTGTCTGC 
GTGCATGGTT TAGATGGTAA CAGTGCAGAT CTCCGACTAG TAAAAACGTA TATTGAACTT 
GGACTGCCTG GGGGAAGAGT TGATTTTCTT ATGTCTGAGA GAAATCAGAA TGATACTTTC 
GCTGATTTTG ATAGCATGAC TGATCGTCTT TTGGATGAAA TCATACAGTA TATTCAGATA 
TATAGTTTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CATTGGGCAA TTTAATAATC 
CGTTCAGTGC TCACAAGGCC ACGGTTTAAA TATTACCTCA GCAAACTTCA TACCTTTCTC 
TCTCTTTCTG GGCCTCACCT TGGTACACTG TACAATAGCA GTGCTCTCGT CAATACAGGT 
CTCTGGTTCA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TGCAACTGAC ATGTCGAGAT 
CATTCAGACC CTCGCCAAAC TTTTTTATAT AAGCTTAGTA ATAAAGCAGG GCTTCATTAT 
TTCAAGAATG TTGTGCTGGT AGGATCACTA CAGGATCGCT ATGTTCCATA TCATTCTGCC 
CGCATTGAAA TGTGCAAAAC AGCCTTGAAG GACAAACAGT CAGGACAAAT CTATTCAGAA 
ATGATCCACA ACTTGCTTCG CCCAGTTCTG CAAAGCAAAG ACTGTAATTT GATTCGATAC 
AATGTCATCA ATGCATTGCC CAACACGGCT GATTCACTCA TCGGGAGAGC TGCACACATA 
GCTGTTCTTG ATTCGGAAAT ATTTTTAGAG AAGTTCTTCC TGGTTGCTGC CCTCAAATAT 
TTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_029557804.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413664.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X3, mRNA.

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CloneID	ONa138118
Clone ID Related Accession (Same CDS sequence)	XM_029557803.1 , XM_029557804.1 , XM_029557805.1
Accession Version	XM_029557804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGACTT AGATGCACCC TGGACGGGAA TTCAGACTCT TCAGATATCA 
GAGTCCAGTA GAATGGATAA ATATGAGACT GAAGAAAGCT CTGTAGCAGG ACTTTCTAGC 
CCAGAGTTGA AAGTCAGAAC TGCTGGTGCC TCCAGTATAT GCTGTACAGA TGGTGAAAAG 
CAGCTAACAA AGTCTCAAAA AGGAAAGAAT GAAGAATTAA ATAAATCCAA AGTTAAGGTC 
ACTAAGCTCA TGAAAACAAT GAAACAAGAG AACACAAAAA AGTTAATAAA ACAGAACTCT 
AAAGATTCTG TGATTTTAGT AAGCTACAGA TGTTTGAAAA GTACACCATC AAGTGATCTT 
AGTAAATGCT TTGAAGGCAA TCCTGCACAT AGTCAGAAAG AAGGTCTGGA TCCCACAATA 
TGTGGATATA ATTTTGACCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TCTTTGCCAG CTAATACAGA GAGAACTGAA CAAAAGTCTC CAGATGTTGA AAATGTGCAA 
CCAGACCAGT TTGATCCCTT GAACTCTGGC AGCCTAAATC TTTGTGCGCA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGACGAT AGTGATATCA CACACATGGA ACATAATCTG 
GCATCAAGAA GTTCATCAGA TGATTTCCAT GACTGTCAGA CAACCCCATC TTCAGCAGTT 
AAAACACTTG AAGTAAATTC CACCAATAAA AATCCTTTCG ATGGAGAGAA AATAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTAGATAA TTTTCTATCC 
ACTTTTGAAT CCTTAGACTC TAATGGTAAA TCCAAGTCTA TAGAAATAAC ACTTGAAAAG 
GAGGCTTTAC AGGAAGCAAA ATGTCATTCT TTTGGAGAAT CATTAGCTAA ATTAAGAAAT 
AATCTACCTG CTCCTACAAA AGAATATCAT GTTGTAGTAA GTGGAGATAC AATTAAATTA 
CCAGATACTA ATGCCACATA TGCCTCATCT AGATTTTCAG ATTCAGGTGT TGAAAGTGAA 
CCAAGCTCTT TTGCAACACA TCCAAACCTG GAAATAGTCT CTGAAACTGG CCAAGGGCAA 
GGTCCTTACC ATAATGAAAG GTTATTTCCT CAGCTTTTGA TGAAACCTGA TTACAATGTC 
AAGTTTTCAT TAGGAAGTCA CTGTACTGAG AGTACAAGTG CTTTAAGTGA AATACAGTCA 
TCTTTGACAT CAATAAACTC TCTACCTTCT GATGATGAAC TCTCACCTGA TGAAAATTCT 
AAGAAATCTT CTGTGCCTGA ATGCCATCTA AGTGATAGTA AAACTGTATT AAATCTAGGA 
ACAATGGATT TGCCAAAGTG TGATGATACT AAAAAGTCAA GTATTATTTT GCAGCCTCAA 
TGTGTTGTAT TTTCAGGGCA TTTGGACAAT GAAACTTTAG CAATACATTC CTTAAATTCA 
AGCACTGAAG ACCCTTTACA ACATGTTTTT TCAGATAAAG ATACTTCTAG TGATGTGAAA 
AGTAGTTGTA GCTCCCAGCC TAATTTGGAC CCTATATTTA AAGGCTCCCA GAGTCATGAT 
ACATCTAATA ACAACTCTGC AGGAACCGTG ATCGCAATAC AGTCAGAACT GATTTGCTTA 
GGCACTCCTT GTGTTGTTTC GGGTTCTGTT TCTACTAATT CAGAACTAAG TCAAGAGGGA 
ACTGTGGAAA GAAAAAGTAG TGACCCATTA AGTCTCAAAC AAATGTATTC AGAAGCCCCT 
TCAATTGAAC ATGAACTTCA TCTGGGTTCG AGTGACCCTC TTTCAGCCAG TACTGATATG 
GTAAAAGAAG GGCTTGTGGA AAATTATTTT GGTTCTCAGA GCAGTACAGA TATTTCGGAC 
ATACGTGCTG TTAGCTACCA CAACTCAGTT AGCCCTCAGA AGGAAACCTG TGAAAAAGGA 
ATCAGTGACC TTCAGCAGGA ACAGGGCAAA GATGAGGAGG AGGAAGAACA GGATCAACAA 
ATGATTCAAA ATGGGTATTA TGAAGAAACA GATTTTTCAG CTTTGGGTGG AACTATAAAT 
GCTCACTTTC CCAGCAGAGA TGTGCAAACA GAGGAAAGGC ATCTAAAATC TGAAGAAGTG 
AACAGTGACT ATCTGAGGGA CAGTGTGAAT GTGCCTACTG TTTGTACTTC GGGTTGTTTG 
TCTTTCCCTT CTGCTCCACG AGAGTCTCCT TGTAGTGTTA AATACTCTTC TAGAAGTAAA 
TTTGATGCCA TTACAAAGCA GCCAAGCAGC ATTTCTTACA ACTTCACTTC TTCAATTTCC 
TGGTATGAAA ACTCACCAAA ACCTCAAATA CAAGCCTTCC TTCAGGCAAA AGAAGAACTG 
AAGCAACTTA AACTCCCTGG GTTCATGTAC AGTGATGTTC CTCTGCTGGC ATCCTCAGTA 
CCTTATTTCA GCATGGAAGA AGAAGATGGT TCTGAAGATG GTGTCCATCT CATTGTCTGC 
GTGCATGGTT TAGATGGTAA CAGTGCAGAT CTCCGACTAG TAAAAACGTA TATTGAACTT 
GGACTGCCTG GGGGAAGAGT TGATTTTCTT ATGTCTGAGA GAAATCAGAA TGATACTTTC 
GCTGATTTTG ATAGCATGAC TGATCGTCTT TTGGATGAAA TCATACAGTA TATTCAGATA 
TATAGTTTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CATTGGGCAA TTTAATAATC 
CGTTCAGTGC TCACAAGGCC ACGGTTTAAA TATTACCTCA GCAAACTTCA TACCTTTCTC 
TCTCTTTCTG GGCCTCACCT TGGTACACTG TACAATAGCA GTGCTCTCGT CAATACAGGT 
CTCTGGTTCA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TGCAACTGAC ATGTCGAGAT 
CATTCAGACC CTCGCCAAAC TTTTTTATAT AAGCTTAGTA ATAAAGCAGG GCTTCATTAT 
TTCAAGAATG TTGTGCTGGT AGGATCACTA CAGGATCGCT ATGTTCCATA TCATTCTGCC 
CGCATTGAAA TGTGCAAAAC AGCCTTGAAG GACAAACAGT CAGGACAAAT CTATTCAGAA 
ATGATCCACA ACTTGCTTCG CCCAGTTCTG CAAAGCAAAG ACTGTAATTT GATTCGATAC 
AATGTCATCA ATGCATTGCC CAACACGGCT GATTCACTCA TCGGGAGAGC TGCACACATA 
GCTGTTCTTG ATTCGGAAAT ATTTTTAGAG AAGTTCTTCC TGGTTGCTGC CCTCAAATAT 
TTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413664.1
CDS	444..5072
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557804.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557804.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGACTT AGATGCACCC TGGACGGGAA TTCAGACTCT TCAGATATCA 
GAGTCCAGTA GAATGGATAA ATATGAGACT GAAGAAAGCT CTGTAGCAGG ACTTTCTAGC 
CCAGAGTTGA AAGTCAGAAC TGCTGGTGCC TCCAGTATAT GCTGTACAGA TGGTGAAAAG 
CAGCTAACAA AGTCTCAAAA AGGAAAGAAT GAAGAATTAA ATAAATCCAA AGTTAAGGTC 
ACTAAGCTCA TGAAAACAAT GAAACAAGAG AACACAAAAA AGTTAATAAA ACAGAACTCT 
AAAGATTCTG TGATTTTAGT AAGCTACAGA TGTTTGAAAA GTACACCATC AAGTGATCTT 
AGTAAATGCT TTGAAGGCAA TCCTGCACAT AGTCAGAAAG AAGGTCTGGA TCCCACAATA 
TGTGGATATA ATTTTGACCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TCTTTGCCAG CTAATACAGA GAGAACTGAA CAAAAGTCTC CAGATGTTGA AAATGTGCAA 
CCAGACCAGT TTGATCCCTT GAACTCTGGC AGCCTAAATC TTTGTGCGCA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGACGAT AGTGATATCA CACACATGGA ACATAATCTG 
GCATCAAGAA GTTCATCAGA TGATTTCCAT GACTGTCAGA CAACCCCATC TTCAGCAGTT 
AAAACACTTG AAGTAAATTC CACCAATAAA AATCCTTTCG ATGGAGAGAA AATAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTAGATAA TTTTCTATCC 
ACTTTTGAAT CCTTAGACTC TAATGGTAAA TCCAAGTCTA TAGAAATAAC ACTTGAAAAG 
GAGGCTTTAC AGGAAGCAAA ATGTCATTCT TTTGGAGAAT CATTAGCTAA ATTAAGAAAT 
AATCTACCTG CTCCTACAAA AGAATATCAT GTTGTAGTAA GTGGAGATAC AATTAAATTA 
CCAGATACTA ATGCCACATA TGCCTCATCT AGATTTTCAG ATTCAGGTGT TGAAAGTGAA 
CCAAGCTCTT TTGCAACACA TCCAAACCTG GAAATAGTCT CTGAAACTGG CCAAGGGCAA 
GGTCCTTACC ATAATGAAAG GTTATTTCCT CAGCTTTTGA TGAAACCTGA TTACAATGTC 
AAGTTTTCAT TAGGAAGTCA CTGTACTGAG AGTACAAGTG CTTTAAGTGA AATACAGTCA 
TCTTTGACAT CAATAAACTC TCTACCTTCT GATGATGAAC TCTCACCTGA TGAAAATTCT 
AAGAAATCTT CTGTGCCTGA ATGCCATCTA AGTGATAGTA AAACTGTATT AAATCTAGGA 
ACAATGGATT TGCCAAAGTG TGATGATACT AAAAAGTCAA GTATTATTTT GCAGCCTCAA 
TGTGTTGTAT TTTCAGGGCA TTTGGACAAT GAAACTTTAG CAATACATTC CTTAAATTCA 
AGCACTGAAG ACCCTTTACA ACATGTTTTT TCAGATAAAG ATACTTCTAG TGATGTGAAA 
AGTAGTTGTA GCTCCCAGCC TAATTTGGAC CCTATATTTA AAGGCTCCCA GAGTCATGAT 
ACATCTAATA ACAACTCTGC AGGAACCGTG ATCGCAATAC AGTCAGAACT GATTTGCTTA 
GGCACTCCTT GTGTTGTTTC GGGTTCTGTT TCTACTAATT CAGAACTAAG TCAAGAGGGA 
ACTGTGGAAA GAAAAAGTAG TGACCCATTA AGTCTCAAAC AAATGTATTC AGAAGCCCCT 
TCAATTGAAC ATGAACTTCA TCTGGGTTCG AGTGACCCTC TTTCAGCCAG TACTGATATG 
GTAAAAGAAG GGCTTGTGGA AAATTATTTT GGTTCTCAGA GCAGTACAGA TATTTCGGAC 
ATACGTGCTG TTAGCTACCA CAACTCAGTT AGCCCTCAGA AGGAAACCTG TGAAAAAGGA 
ATCAGTGACC TTCAGCAGGA ACAGGGCAAA GATGAGGAGG AGGAAGAACA GGATCAACAA 
ATGATTCAAA ATGGGTATTA TGAAGAAACA GATTTTTCAG CTTTGGGTGG AACTATAAAT 
GCTCACTTTC CCAGCAGAGA TGTGCAAACA GAGGAAAGGC ATCTAAAATC TGAAGAAGTG 
AACAGTGACT ATCTGAGGGA CAGTGTGAAT GTGCCTACTG TTTGTACTTC GGGTTGTTTG 
TCTTTCCCTT CTGCTCCACG AGAGTCTCCT TGTAGTGTTA AATACTCTTC TAGAAGTAAA 
TTTGATGCCA TTACAAAGCA GCCAAGCAGC ATTTCTTACA ACTTCACTTC TTCAATTTCC 
TGGTATGAAA ACTCACCAAA ACCTCAAATA CAAGCCTTCC TTCAGGCAAA AGAAGAACTG 
AAGCAACTTA AACTCCCTGG GTTCATGTAC AGTGATGTTC CTCTGCTGGC ATCCTCAGTA 
CCTTATTTCA GCATGGAAGA AGAAGATGGT TCTGAAGATG GTGTCCATCT CATTGTCTGC 
GTGCATGGTT TAGATGGTAA CAGTGCAGAT CTCCGACTAG TAAAAACGTA TATTGAACTT 
GGACTGCCTG GGGGAAGAGT TGATTTTCTT ATGTCTGAGA GAAATCAGAA TGATACTTTC 
GCTGATTTTG ATAGCATGAC TGATCGTCTT TTGGATGAAA TCATACAGTA TATTCAGATA 
TATAGTTTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CATTGGGCAA TTTAATAATC 
CGTTCAGTGC TCACAAGGCC ACGGTTTAAA TATTACCTCA GCAAACTTCA TACCTTTCTC 
TCTCTTTCTG GGCCTCACCT TGGTACACTG TACAATAGCA GTGCTCTCGT CAATACAGGT 
CTCTGGTTCA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TGCAACTGAC ATGTCGAGAT 
CATTCAGACC CTCGCCAAAC TTTTTTATAT AAGCTTAGTA ATAAAGCAGG GCTTCATTAT 
TTCAAGAATG TTGTGCTGGT AGGATCACTA CAGGATCGCT ATGTTCCATA TCATTCTGCC 
CGCATTGAAA TGTGCAAAAC AGCCTTGAAG GACAAACAGT CAGGACAAAT CTATTCAGAA 
ATGATCCACA ACTTGCTTCG CCCAGTTCTG CAAAGCAAAG ACTGTAATTT GATTCGATAC 
AATGTCATCA ATGCATTGCC CAACACGGCT GATTCACTCA TCGGGAGAGC TGCACACATA 
GCTGTTCTTG ATTCGGAAAT ATTTTTAGAG AAGTTCTTCC TGGTTGCTGC CCTCAAATAT 
TTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_029557805.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413665.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X4, mRNA.

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CloneID	ONa138118
Clone ID Related Accession (Same CDS sequence)	XM_029557803.1 , XM_029557804.1 , XM_029557805.1
Accession Version	XM_029557805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGACTT AGATGCACCC TGGACGGGAA TTCAGACTCT TCAGATATCA 
GAGTCCAGTA GAATGGATAA ATATGAGACT GAAGAAAGCT CTGTAGCAGG ACTTTCTAGC 
CCAGAGTTGA AAGTCAGAAC TGCTGGTGCC TCCAGTATAT GCTGTACAGA TGGTGAAAAG 
CAGCTAACAA AGTCTCAAAA AGGAAAGAAT GAAGAATTAA ATAAATCCAA AGTTAAGGTC 
ACTAAGCTCA TGAAAACAAT GAAACAAGAG AACACAAAAA AGTTAATAAA ACAGAACTCT 
AAAGATTCTG TGATTTTAGT AAGCTACAGA TGTTTGAAAA GTACACCATC AAGTGATCTT 
AGTAAATGCT TTGAAGGCAA TCCTGCACAT AGTCAGAAAG AAGGTCTGGA TCCCACAATA 
TGTGGATATA ATTTTGACCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TCTTTGCCAG CTAATACAGA GAGAACTGAA CAAAAGTCTC CAGATGTTGA AAATGTGCAA 
CCAGACCAGT TTGATCCCTT GAACTCTGGC AGCCTAAATC TTTGTGCGCA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGACGAT AGTGATATCA CACACATGGA ACATAATCTG 
GCATCAAGAA GTTCATCAGA TGATTTCCAT GACTGTCAGA CAACCCCATC TTCAGCAGTT 
AAAACACTTG AAGTAAATTC CACCAATAAA AATCCTTTCG ATGGAGAGAA AATAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTAGATAA TTTTCTATCC 
ACTTTTGAAT CCTTAGACTC TAATGGTAAA TCCAAGTCTA TAGAAATAAC ACTTGAAAAG 
GAGGCTTTAC AGGAAGCAAA ATGTCATTCT TTTGGAGAAT CATTAGCTAA ATTAAGAAAT 
AATCTACCTG CTCCTACAAA AGAATATCAT GTTGTAGTAA GTGGAGATAC AATTAAATTA 
CCAGATACTA ATGCCACATA TGCCTCATCT AGATTTTCAG ATTCAGGTGT TGAAAGTGAA 
CCAAGCTCTT TTGCAACACA TCCAAACCTG GAAATAGTCT CTGAAACTGG CCAAGGGCAA 
GGTCCTTACC ATAATGAAAG GTTATTTCCT CAGCTTTTGA TGAAACCTGA TTACAATGTC 
AAGTTTTCAT TAGGAAGTCA CTGTACTGAG AGTACAAGTG CTTTAAGTGA AATACAGTCA 
TCTTTGACAT CAATAAACTC TCTACCTTCT GATGATGAAC TCTCACCTGA TGAAAATTCT 
AAGAAATCTT CTGTGCCTGA ATGCCATCTA AGTGATAGTA AAACTGTATT AAATCTAGGA 
ACAATGGATT TGCCAAAGTG TGATGATACT AAAAAGTCAA GTATTATTTT GCAGCCTCAA 
TGTGTTGTAT TTTCAGGGCA TTTGGACAAT GAAACTTTAG CAATACATTC CTTAAATTCA 
AGCACTGAAG ACCCTTTACA ACATGTTTTT TCAGATAAAG ATACTTCTAG TGATGTGAAA 
AGTAGTTGTA GCTCCCAGCC TAATTTGGAC CCTATATTTA AAGGCTCCCA GAGTCATGAT 
ACATCTAATA ACAACTCTGC AGGAACCGTG ATCGCAATAC AGTCAGAACT GATTTGCTTA 
GGCACTCCTT GTGTTGTTTC GGGTTCTGTT TCTACTAATT CAGAACTAAG TCAAGAGGGA 
ACTGTGGAAA GAAAAAGTAG TGACCCATTA AGTCTCAAAC AAATGTATTC AGAAGCCCCT 
TCAATTGAAC ATGAACTTCA TCTGGGTTCG AGTGACCCTC TTTCAGCCAG TACTGATATG 
GTAAAAGAAG GGCTTGTGGA AAATTATTTT GGTTCTCAGA GCAGTACAGA TATTTCGGAC 
ATACGTGCTG TTAGCTACCA CAACTCAGTT AGCCCTCAGA AGGAAACCTG TGAAAAAGGA 
ATCAGTGACC TTCAGCAGGA ACAGGGCAAA GATGAGGAGG AGGAAGAACA GGATCAACAA 
ATGATTCAAA ATGGGTATTA TGAAGAAACA GATTTTTCAG CTTTGGGTGG AACTATAAAT 
GCTCACTTTC CCAGCAGAGA TGTGCAAACA GAGGAAAGGC ATCTAAAATC TGAAGAAGTG 
AACAGTGACT ATCTGAGGGA CAGTGTGAAT GTGCCTACTG TTTGTACTTC GGGTTGTTTG 
TCTTTCCCTT CTGCTCCACG AGAGTCTCCT TGTAGTGTTA AATACTCTTC TAGAAGTAAA 
TTTGATGCCA TTACAAAGCA GCCAAGCAGC ATTTCTTACA ACTTCACTTC TTCAATTTCC 
TGGTATGAAA ACTCACCAAA ACCTCAAATA CAAGCCTTCC TTCAGGCAAA AGAAGAACTG 
AAGCAACTTA AACTCCCTGG GTTCATGTAC AGTGATGTTC CTCTGCTGGC ATCCTCAGTA 
CCTTATTTCA GCATGGAAGA AGAAGATGGT TCTGAAGATG GTGTCCATCT CATTGTCTGC 
GTGCATGGTT TAGATGGTAA CAGTGCAGAT CTCCGACTAG TAAAAACGTA TATTGAACTT 
GGACTGCCTG GGGGAAGAGT TGATTTTCTT ATGTCTGAGA GAAATCAGAA TGATACTTTC 
GCTGATTTTG ATAGCATGAC TGATCGTCTT TTGGATGAAA TCATACAGTA TATTCAGATA 
TATAGTTTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CATTGGGCAA TTTAATAATC 
CGTTCAGTGC TCACAAGGCC ACGGTTTAAA TATTACCTCA GCAAACTTCA TACCTTTCTC 
TCTCTTTCTG GGCCTCACCT TGGTACACTG TACAATAGCA GTGCTCTCGT CAATACAGGT 
CTCTGGTTCA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TGCAACTGAC ATGTCGAGAT 
CATTCAGACC CTCGCCAAAC TTTTTTATAT AAGCTTAGTA ATAAAGCAGG GCTTCATTAT 
TTCAAGAATG TTGTGCTGGT AGGATCACTA CAGGATCGCT ATGTTCCATA TCATTCTGCC 
CGCATTGAAA TGTGCAAAAC AGCCTTGAAG GACAAACAGT CAGGACAAAT CTATTCAGAA 
ATGATCCACA ACTTGCTTCG CCCAGTTCTG CAAAGCAAAG ACTGTAATTT GATTCGATAC 
AATGTCATCA ATGCATTGCC CAACACGGCT GATTCACTCA TCGGGAGAGC TGCACACATA 
GCTGTTCTTG ATTCGGAAAT ATTTTTAGAG AAGTTCTTCC TGGTTGCTGC CCTCAAATAT 
TTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413665.1
CDS	465..5093
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557805.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557805.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGCCAAGGC CAACATGCAG 
GTCCTTTATG AACGCCTCCT CAGAAGAAAA CAGCCAAGAA CCCAGAATGA CACCTGTGTA 
GATAAAATGG ATGTAGAGGC TCGTCTTGCT GAACTGTGTG AAGAAGTTAA AAAAATAGAA 
AATCCTGATG AGCTGGCAGA ACTGATAAAT ATGAATCTTG CACAACTTTG CTCACTTCTC 
ATGGCTTTAT GGGGACAGTT TCTGGAAGTT ATAACACTAC ATGAAGAACT AAGAGTGTTA 
CTAGCACAGG AGCACCATAC TTTACGGGTT CGCAGGTTTT CTGAGGCATT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTCC ATGCTCAGAG TCATCTACAG 
ATGTGCACCG CTATCAAAAA TACTTCCTTC TGCAGTTCTC TTCCACCCCT ACCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCA TTACCTATAA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA TTGAAGACTT AGATGCACCC TGGACGGGAA TTCAGACTCT TCAGATATCA 
GAGTCCAGTA GAATGGATAA ATATGAGACT GAAGAAAGCT CTGTAGCAGG ACTTTCTAGC 
CCAGAGTTGA AAGTCAGAAC TGCTGGTGCC TCCAGTATAT GCTGTACAGA TGGTGAAAAG 
CAGCTAACAA AGTCTCAAAA AGGAAAGAAT GAAGAATTAA ATAAATCCAA AGTTAAGGTC 
ACTAAGCTCA TGAAAACAAT GAAACAAGAG AACACAAAAA AGTTAATAAA ACAGAACTCT 
AAAGATTCTG TGATTTTAGT AAGCTACAGA TGTTTGAAAA GTACACCATC AAGTGATCTT 
AGTAAATGCT TTGAAGGCAA TCCTGCACAT AGTCAGAAAG AAGGTCTGGA TCCCACAATA 
TGTGGATATA ATTTTGACCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TCTTTGCCAG CTAATACAGA GAGAACTGAA CAAAAGTCTC CAGATGTTGA AAATGTGCAA 
CCAGACCAGT TTGATCCCTT GAACTCTGGC AGCCTAAATC TTTGTGCGCA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGACGAT AGTGATATCA CACACATGGA ACATAATCTG 
GCATCAAGAA GTTCATCAGA TGATTTCCAT GACTGTCAGA CAACCCCATC TTCAGCAGTT 
AAAACACTTG AAGTAAATTC CACCAATAAA AATCCTTTCG ATGGAGAGAA AATAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTAGATAA TTTTCTATCC 
ACTTTTGAAT CCTTAGACTC TAATGGTAAA TCCAAGTCTA TAGAAATAAC ACTTGAAAAG 
GAGGCTTTAC AGGAAGCAAA ATGTCATTCT TTTGGAGAAT CATTAGCTAA ATTAAGAAAT 
AATCTACCTG CTCCTACAAA AGAATATCAT GTTGTAGTAA GTGGAGATAC AATTAAATTA 
CCAGATACTA ATGCCACATA TGCCTCATCT AGATTTTCAG ATTCAGGTGT TGAAAGTGAA 
CCAAGCTCTT TTGCAACACA TCCAAACCTG GAAATAGTCT CTGAAACTGG CCAAGGGCAA 
GGTCCTTACC ATAATGAAAG GTTATTTCCT CAGCTTTTGA TGAAACCTGA TTACAATGTC 
AAGTTTTCAT TAGGAAGTCA CTGTACTGAG AGTACAAGTG CTTTAAGTGA AATACAGTCA 
TCTTTGACAT CAATAAACTC TCTACCTTCT GATGATGAAC TCTCACCTGA TGAAAATTCT 
AAGAAATCTT CTGTGCCTGA ATGCCATCTA AGTGATAGTA AAACTGTATT AAATCTAGGA 
ACAATGGATT TGCCAAAGTG TGATGATACT AAAAAGTCAA GTATTATTTT GCAGCCTCAA 
TGTGTTGTAT TTTCAGGGCA TTTGGACAAT GAAACTTTAG CAATACATTC CTTAAATTCA 
AGCACTGAAG ACCCTTTACA ACATGTTTTT TCAGATAAAG ATACTTCTAG TGATGTGAAA 
AGTAGTTGTA GCTCCCAGCC TAATTTGGAC CCTATATTTA AAGGCTCCCA GAGTCATGAT 
ACATCTAATA ACAACTCTGC AGGAACCGTG ATCGCAATAC AGTCAGAACT GATTTGCTTA 
GGCACTCCTT GTGTTGTTTC GGGTTCTGTT TCTACTAATT CAGAACTAAG TCAAGAGGGA 
ACTGTGGAAA GAAAAAGTAG TGACCCATTA AGTCTCAAAC AAATGTATTC AGAAGCCCCT 
TCAATTGAAC ATGAACTTCA TCTGGGTTCG AGTGACCCTC TTTCAGCCAG TACTGATATG 
GTAAAAGAAG GGCTTGTGGA AAATTATTTT GGTTCTCAGA GCAGTACAGA TATTTCGGAC 
ATACGTGCTG TTAGCTACCA CAACTCAGTT AGCCCTCAGA AGGAAACCTG TGAAAAAGGA 
ATCAGTGACC TTCAGCAGGA ACAGGGCAAA GATGAGGAGG AGGAAGAACA GGATCAACAA 
ATGATTCAAA ATGGGTATTA TGAAGAAACA GATTTTTCAG CTTTGGGTGG AACTATAAAT 
GCTCACTTTC CCAGCAGAGA TGTGCAAACA GAGGAAAGGC ATCTAAAATC TGAAGAAGTG 
AACAGTGACT ATCTGAGGGA CAGTGTGAAT GTGCCTACTG TTTGTACTTC GGGTTGTTTG 
TCTTTCCCTT CTGCTCCACG AGAGTCTCCT TGTAGTGTTA AATACTCTTC TAGAAGTAAA 
TTTGATGCCA TTACAAAGCA GCCAAGCAGC ATTTCTTACA ACTTCACTTC TTCAATTTCC 
TGGTATGAAA ACTCACCAAA ACCTCAAATA CAAGCCTTCC TTCAGGCAAA AGAAGAACTG 
AAGCAACTTA AACTCCCTGG GTTCATGTAC AGTGATGTTC CTCTGCTGGC ATCCTCAGTA 
CCTTATTTCA GCATGGAAGA AGAAGATGGT TCTGAAGATG GTGTCCATCT CATTGTCTGC 
GTGCATGGTT TAGATGGTAA CAGTGCAGAT CTCCGACTAG TAAAAACGTA TATTGAACTT 
GGACTGCCTG GGGGAAGAGT TGATTTTCTT ATGTCTGAGA GAAATCAGAA TGATACTTTC 
GCTGATTTTG ATAGCATGAC TGATCGTCTT TTGGATGAAA TCATACAGTA TATTCAGATA 
TATAGTTTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CATTGGGCAA TTTAATAATC 
CGTTCAGTGC TCACAAGGCC ACGGTTTAAA TATTACCTCA GCAAACTTCA TACCTTTCTC 
TCTCTTTCTG GGCCTCACCT TGGTACACTG TACAATAGCA GTGCTCTCGT CAATACAGGT 
CTCTGGTTCA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TGCAACTGAC ATGTCGAGAT 
CATTCAGACC CTCGCCAAAC TTTTTTATAT AAGCTTAGTA ATAAAGCAGG GCTTCATTAT 
TTCAAGAATG TTGTGCTGGT AGGATCACTA CAGGATCGCT ATGTTCCATA TCATTCTGCC 
CGCATTGAAA TGTGCAAAAC AGCCTTGAAG GACAAACAGT CAGGACAAAT CTATTCAGAA 
ATGATCCACA ACTTGCTTCG CCCAGTTCTG CAAAGCAAAG ACTGTAATTT GATTCGATAC 
AATGTCATCA ATGCATTGCC CAACACGGCT GATTCACTCA TCGGGAGAGC TGCACACATA 
GCTGTTCTTG ATTCGGAAAT ATTTTTAGAG AAGTTCTTCC TGGTTGCTGC CCTCAAATAT 
TTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_029557807.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413667.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ONa138119
Clone ID Related Accession (Same CDS sequence)	XM_029557807.1 , XM_029557806.1
Accession Version	XM_029557807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGAC 
TTAGATGCAC CCTGGACGGG AATTCAGACT CTTCAGATAT CAGAGTCCAG TAGAATGGAT 
AAATATGAGA CTGAAGAAAG CTCTGTAGCA GGACTTTCTA GCCCAGAGTT GAAAGTCAGA 
ACTGCTGGTG CCTCCAGTAT ATGCTGTACA GATGGTGAAA AGCAGCTAAC AAAGTCTCAA 
AAAGGAAAGA ATGAAGAATT AAATAAATCC AAAGTTAAGG TCACTAAGCT CATGAAAACA 
ATGAAACAAG AGAACACAAA AAAGTTAATA AAACAGAACT CTAAAGATTC TGTGATTTTA 
GTAAGCTACA GATGTTTGAA AAGTACACCA TCAAGTGATC TTAGTAAATG CTTTGAAGGC 
AATCCTGCAC ATAGTCAGAA AGAAGGTCTG GATCCCACAA TATGTGGATA TAATTTTGAC 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTCTTTGCC AGCTAATACA 
GAGAGAACTG AACAAAAGTC TCCAGATGTT GAAAATGTGC AACCAGACCA GTTTGATCCC 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCG CATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGACG ATAGTGATAT CACACACATG GAACATAATC TGGCATCAAG AAGTTCATCA 
GATGATTTCC ATGACTGTCA GACAACCCCA TCTTCAGCAG TTAAAACACT TGAAGTAAAT 
TCCACCAATA AAAATCCTTT CGATGGAGAG AAAATAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTAGAT AATTTTCTAT CCACTTTTGA ATCCTTAGAC 
TCTAATGGTA AATCCAAGTC TATAGAAATA ACACTTGAAA AGGAGGCTTT ACAGGAAGCA 
AAATGTCATT CTTTTGGAGA ATCATTAGCT AAATTAAGAA ATAATCTACC TGCTCCTACA 
AAAGAATATC ATGTTGTAGT AAGTGGAGAT ACAATTAAAT TACCAGATAC TAATGCCACA 
TATGCCTCAT CTAGATTTTC AGATTCAGGT GTTGAAAGTG AACCAAGCTC TTTTGCAACA 
CATCCAAACC TGGAAATAGT CTCTGAAACT GGCCAAGGGC AAGGTCCTTA CCATAATGAA 
AGGTTATTTC CTCAGCTTTT GATGAAACCT GATTACAATG TCAAGTTTTC ATTAGGAAGT 
CACTGTACTG AGAGTACAAG TGCTTTAAGT GAAATACAGT CATCTTTGAC ATCAATAAAC 
TCTCTACCTT CTGATGATGA ACTCTCACCT GATGAAAATT CTAAGAAATC TTCTGTGCCT 
GAATGCCATC TAAGTGATAG TAAAACTGTA TTAAATCTAG GAACAATGGA TTTGCCAAAG 
TGTGATGATA CTAAAAAGTC AAGTATTATT TTGCAGCCTC AATGTGTTGT ATTTTCAGGG 
CATTTGGACA ATGAAACTTT AGCAATACAT TCCTTAAATT CAAGCACTGA AGACCCTTTA 
CAACATGTTT TTTCAGATAA AGATACTTCT AGTGATGTGA AAAGTAGTTG TAGCTCCCAG 
CCTAATTTGG ACCCTATATT TAAAGGCTCC CAGAGTCATG ATACATCTAA TAACAACTCT 
GCAGGAACCG TGATCGCAAT ACAGTCAGAA CTGATTTGCT TAGGCACTCC TTGTGTTGTT 
TCGGGTTCTG TTTCTACTAA TTCAGAACTA AGTCAAGAGG GAACTGTGGA AAGAAAAAGT 
AGTGACCCAT TAAGTCTCAA ACAAATGTAT TCAGAAGCCC CTTCAATTGA ACATGAACTT 
CATCTGGGTT CGAGTGACCC TCTTTCAGCC AGTACTGATA TGGTAAAAGA AGGGCTTGTG 
GAAAATTATT TTGGTTCTCA GAGCAGTACA GATATTTCGG ACATACGTGC TGTTAGCTAC 
CACAACTCAG TTAGCCCTCA GAAGGAAACC TGTGAAAAAG GAATCAGTGA CCTTCAGCAG 
GAACAGGGCA AAGATGAGGA GGAGGAAGAA CAGGATCAAC AAATGATTCA AAATGGGTAT 
TATGAAGAAA CAGATTTTTC AGCTTTGGGT GGAACTATAA ATGCTCACTT TCCCAGCAGA 
GATGTGCAAA CAGAGGAAAG GCATCTAAAA TCTGAAGAAG TGAACAGTGA CTATCTGAGG 
GACAGTGTGA ATGTGCCTAC TGTTTGTACT TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA 
CGAGAGTCTC CTTGTAGTGT TAAATACTCT TCTAGAAGTA AATTTGATGC CATTACAAAG 
CAGCCAAGCA GCATTTCTTA CAACTTCACT TCTTCAATTT CCTGGTATGA AAACTCACCA 
AAACCTCAAA TACAAGCCTT CCTTCAGGCA AAAGAAGAAC TGAAGCAACT TAAACTCCCT 
GGGTTCATGT ACAGTGATGT TCCTCTGCTG GCATCCTCAG TACCTTATTT CAGCATGGAA 
GAAGAAGATG GTTCTGAAGA TGGTGTCCAT CTCATTGTCT GCGTGCATGG TTTAGATGGT 
AACAGTGCAG ATCTCCGACT AGTAAAAACG TATATTGAAC TTGGACTGCC TGGGGGAAGA 
GTTGATTTTC TTATGTCTGA GAGAAATCAG AATGATACTT TCGCTGATTT TGATAGCATG 
ACTGATCGTC TTTTGGATGA AATCATACAG TATATTCAGA TATATAGTTT AACAGTCTCA 
AAAATAAGCT TTATTGGACA TTCATTGGGC AATTTAATAA TCCGTTCAGT GCTCACAAGG 
CCACGGTTTA AATATTACCT CAGCAAACTT CATACCTTTC TCTCTCTTTC TGGGCCTCAC 
CTTGGTACAC TGTACAATAG CAGTGCTCTC GTCAATACAG GTCTCTGGTT CATGCAGAAA 
TGGAAAAAAT CAGGTTCTCT TTTGCAACTG ACATGTCGAG ATCATTCAGA CCCTCGCCAA 
ACTTTTTTAT ATAAGCTTAG TAATAAAGCA GGGCTTCATT ATTTCAAGAA TGTTGTGCTG 
GTAGGATCAC TACAGGATCG CTATGTTCCA TATCATTCTG CCCGCATTGA AATGTGCAAA 
ACAGCCTTGA AGGACAAACA GTCAGGACAA ATCTATTCAG AAATGATCCA CAACTTGCTT 
CGCCCAGTTC TGCAAAGCAA AGACTGTAAT TTGATTCGAT ACAATGTCAT CAATGCATTG 
CCCAACACGG CTGATTCACT CATCGGGAGA GCTGCACACA TAGCTGTTCT TGATTCGGAA 
ATATTTTTAG AGAAGTTCTT CCTGGTTGCT GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413667.1
CDS	465..5015
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557807.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557807.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGAC 
TTAGATGCAC CCTGGACGGG AATTCAGACT CTTCAGATAT CAGAGTCCAG TAGAATGGAT 
AAATATGAGA CTGAAGAAAG CTCTGTAGCA GGACTTTCTA GCCCAGAGTT GAAAGTCAGA 
ACTGCTGGTG CCTCCAGTAT ATGCTGTACA GATGGTGAAA AGCAGCTAAC AAAGTCTCAA 
AAAGGAAAGA ATGAAGAATT AAATAAATCC AAAGTTAAGG TCACTAAGCT CATGAAAACA 
ATGAAACAAG AGAACACAAA AAAGTTAATA AAACAGAACT CTAAAGATTC TGTGATTTTA 
GTAAGCTACA GATGTTTGAA AAGTACACCA TCAAGTGATC TTAGTAAATG CTTTGAAGGC 
AATCCTGCAC ATAGTCAGAA AGAAGGTCTG GATCCCACAA TATGTGGATA TAATTTTGAC 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTCTTTGCC AGCTAATACA 
GAGAGAACTG AACAAAAGTC TCCAGATGTT GAAAATGTGC AACCAGACCA GTTTGATCCC 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCG CATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGACG ATAGTGATAT CACACACATG GAACATAATC TGGCATCAAG AAGTTCATCA 
GATGATTTCC ATGACTGTCA GACAACCCCA TCTTCAGCAG TTAAAACACT TGAAGTAAAT 
TCCACCAATA AAAATCCTTT CGATGGAGAG AAAATAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTAGAT AATTTTCTAT CCACTTTTGA ATCCTTAGAC 
TCTAATGGTA AATCCAAGTC TATAGAAATA ACACTTGAAA AGGAGGCTTT ACAGGAAGCA 
AAATGTCATT CTTTTGGAGA ATCATTAGCT AAATTAAGAA ATAATCTACC TGCTCCTACA 
AAAGAATATC ATGTTGTAGT AAGTGGAGAT ACAATTAAAT TACCAGATAC TAATGCCACA 
TATGCCTCAT CTAGATTTTC AGATTCAGGT GTTGAAAGTG AACCAAGCTC TTTTGCAACA 
CATCCAAACC TGGAAATAGT CTCTGAAACT GGCCAAGGGC AAGGTCCTTA CCATAATGAA 
AGGTTATTTC CTCAGCTTTT GATGAAACCT GATTACAATG TCAAGTTTTC ATTAGGAAGT 
CACTGTACTG AGAGTACAAG TGCTTTAAGT GAAATACAGT CATCTTTGAC ATCAATAAAC 
TCTCTACCTT CTGATGATGA ACTCTCACCT GATGAAAATT CTAAGAAATC TTCTGTGCCT 
GAATGCCATC TAAGTGATAG TAAAACTGTA TTAAATCTAG GAACAATGGA TTTGCCAAAG 
TGTGATGATA CTAAAAAGTC AAGTATTATT TTGCAGCCTC AATGTGTTGT ATTTTCAGGG 
CATTTGGACA ATGAAACTTT AGCAATACAT TCCTTAAATT CAAGCACTGA AGACCCTTTA 
CAACATGTTT TTTCAGATAA AGATACTTCT AGTGATGTGA AAAGTAGTTG TAGCTCCCAG 
CCTAATTTGG ACCCTATATT TAAAGGCTCC CAGAGTCATG ATACATCTAA TAACAACTCT 
GCAGGAACCG TGATCGCAAT ACAGTCAGAA CTGATTTGCT TAGGCACTCC TTGTGTTGTT 
TCGGGTTCTG TTTCTACTAA TTCAGAACTA AGTCAAGAGG GAACTGTGGA AAGAAAAAGT 
AGTGACCCAT TAAGTCTCAA ACAAATGTAT TCAGAAGCCC CTTCAATTGA ACATGAACTT 
CATCTGGGTT CGAGTGACCC TCTTTCAGCC AGTACTGATA TGGTAAAAGA AGGGCTTGTG 
GAAAATTATT TTGGTTCTCA GAGCAGTACA GATATTTCGG ACATACGTGC TGTTAGCTAC 
CACAACTCAG TTAGCCCTCA GAAGGAAACC TGTGAAAAAG GAATCAGTGA CCTTCAGCAG 
GAACAGGGCA AAGATGAGGA GGAGGAAGAA CAGGATCAAC AAATGATTCA AAATGGGTAT 
TATGAAGAAA CAGATTTTTC AGCTTTGGGT GGAACTATAA ATGCTCACTT TCCCAGCAGA 
GATGTGCAAA CAGAGGAAAG GCATCTAAAA TCTGAAGAAG TGAACAGTGA CTATCTGAGG 
GACAGTGTGA ATGTGCCTAC TGTTTGTACT TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA 
CGAGAGTCTC CTTGTAGTGT TAAATACTCT TCTAGAAGTA AATTTGATGC CATTACAAAG 
CAGCCAAGCA GCATTTCTTA CAACTTCACT TCTTCAATTT CCTGGTATGA AAACTCACCA 
AAACCTCAAA TACAAGCCTT CCTTCAGGCA AAAGAAGAAC TGAAGCAACT TAAACTCCCT 
GGGTTCATGT ACAGTGATGT TCCTCTGCTG GCATCCTCAG TACCTTATTT CAGCATGGAA 
GAAGAAGATG GTTCTGAAGA TGGTGTCCAT CTCATTGTCT GCGTGCATGG TTTAGATGGT 
AACAGTGCAG ATCTCCGACT AGTAAAAACG TATATTGAAC TTGGACTGCC TGGGGGAAGA 
GTTGATTTTC TTATGTCTGA GAGAAATCAG AATGATACTT TCGCTGATTT TGATAGCATG 
ACTGATCGTC TTTTGGATGA AATCATACAG TATATTCAGA TATATAGTTT AACAGTCTCA 
AAAATAAGCT TTATTGGACA TTCATTGGGC AATTTAATAA TCCGTTCAGT GCTCACAAGG 
CCACGGTTTA AATATTACCT CAGCAAACTT CATACCTTTC TCTCTCTTTC TGGGCCTCAC 
CTTGGTACAC TGTACAATAG CAGTGCTCTC GTCAATACAG GTCTCTGGTT CATGCAGAAA 
TGGAAAAAAT CAGGTTCTCT TTTGCAACTG ACATGTCGAG ATCATTCAGA CCCTCGCCAA 
ACTTTTTTAT ATAAGCTTAG TAATAAAGCA GGGCTTCATT ATTTCAAGAA TGTTGTGCTG 
GTAGGATCAC TACAGGATCG CTATGTTCCA TATCATTCTG CCCGCATTGA AATGTGCAAA 
ACAGCCTTGA AGGACAAACA GTCAGGACAA ATCTATTCAG AAATGATCCA CAACTTGCTT 
CGCCCAGTTC TGCAAAGCAA AGACTGTAAT TTGATTCGAT ACAATGTCAT CAATGCATTG 
CCCAACACGG CTGATTCACT CATCGGGAGA GCTGCACACA TAGCTGTTCT TGATTCGGAA 
ATATTTTTAG AGAAGTTCTT CCTGGTTGCT GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_029557806.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413666.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ONa138119
Clone ID Related Accession (Same CDS sequence)	XM_029557807.1 , XM_029557806.1
Accession Version	XM_029557806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGAC 
TTAGATGCAC CCTGGACGGG AATTCAGACT CTTCAGATAT CAGAGTCCAG TAGAATGGAT 
AAATATGAGA CTGAAGAAAG CTCTGTAGCA GGACTTTCTA GCCCAGAGTT GAAAGTCAGA 
ACTGCTGGTG CCTCCAGTAT ATGCTGTACA GATGGTGAAA AGCAGCTAAC AAAGTCTCAA 
AAAGGAAAGA ATGAAGAATT AAATAAATCC AAAGTTAAGG TCACTAAGCT CATGAAAACA 
ATGAAACAAG AGAACACAAA AAAGTTAATA AAACAGAACT CTAAAGATTC TGTGATTTTA 
GTAAGCTACA GATGTTTGAA AAGTACACCA TCAAGTGATC TTAGTAAATG CTTTGAAGGC 
AATCCTGCAC ATAGTCAGAA AGAAGGTCTG GATCCCACAA TATGTGGATA TAATTTTGAC 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTCTTTGCC AGCTAATACA 
GAGAGAACTG AACAAAAGTC TCCAGATGTT GAAAATGTGC AACCAGACCA GTTTGATCCC 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCG CATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGACG ATAGTGATAT CACACACATG GAACATAATC TGGCATCAAG AAGTTCATCA 
GATGATTTCC ATGACTGTCA GACAACCCCA TCTTCAGCAG TTAAAACACT TGAAGTAAAT 
TCCACCAATA AAAATCCTTT CGATGGAGAG AAAATAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTAGAT AATTTTCTAT CCACTTTTGA ATCCTTAGAC 
TCTAATGGTA AATCCAAGTC TATAGAAATA ACACTTGAAA AGGAGGCTTT ACAGGAAGCA 
AAATGTCATT CTTTTGGAGA ATCATTAGCT AAATTAAGAA ATAATCTACC TGCTCCTACA 
AAAGAATATC ATGTTGTAGT AAGTGGAGAT ACAATTAAAT TACCAGATAC TAATGCCACA 
TATGCCTCAT CTAGATTTTC AGATTCAGGT GTTGAAAGTG AACCAAGCTC TTTTGCAACA 
CATCCAAACC TGGAAATAGT CTCTGAAACT GGCCAAGGGC AAGGTCCTTA CCATAATGAA 
AGGTTATTTC CTCAGCTTTT GATGAAACCT GATTACAATG TCAAGTTTTC ATTAGGAAGT 
CACTGTACTG AGAGTACAAG TGCTTTAAGT GAAATACAGT CATCTTTGAC ATCAATAAAC 
TCTCTACCTT CTGATGATGA ACTCTCACCT GATGAAAATT CTAAGAAATC TTCTGTGCCT 
GAATGCCATC TAAGTGATAG TAAAACTGTA TTAAATCTAG GAACAATGGA TTTGCCAAAG 
TGTGATGATA CTAAAAAGTC AAGTATTATT TTGCAGCCTC AATGTGTTGT ATTTTCAGGG 
CATTTGGACA ATGAAACTTT AGCAATACAT TCCTTAAATT CAAGCACTGA AGACCCTTTA 
CAACATGTTT TTTCAGATAA AGATACTTCT AGTGATGTGA AAAGTAGTTG TAGCTCCCAG 
CCTAATTTGG ACCCTATATT TAAAGGCTCC CAGAGTCATG ATACATCTAA TAACAACTCT 
GCAGGAACCG TGATCGCAAT ACAGTCAGAA CTGATTTGCT TAGGCACTCC TTGTGTTGTT 
TCGGGTTCTG TTTCTACTAA TTCAGAACTA AGTCAAGAGG GAACTGTGGA AAGAAAAAGT 
AGTGACCCAT TAAGTCTCAA ACAAATGTAT TCAGAAGCCC CTTCAATTGA ACATGAACTT 
CATCTGGGTT CGAGTGACCC TCTTTCAGCC AGTACTGATA TGGTAAAAGA AGGGCTTGTG 
GAAAATTATT TTGGTTCTCA GAGCAGTACA GATATTTCGG ACATACGTGC TGTTAGCTAC 
CACAACTCAG TTAGCCCTCA GAAGGAAACC TGTGAAAAAG GAATCAGTGA CCTTCAGCAG 
GAACAGGGCA AAGATGAGGA GGAGGAAGAA CAGGATCAAC AAATGATTCA AAATGGGTAT 
TATGAAGAAA CAGATTTTTC AGCTTTGGGT GGAACTATAA ATGCTCACTT TCCCAGCAGA 
GATGTGCAAA CAGAGGAAAG GCATCTAAAA TCTGAAGAAG TGAACAGTGA CTATCTGAGG 
GACAGTGTGA ATGTGCCTAC TGTTTGTACT TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA 
CGAGAGTCTC CTTGTAGTGT TAAATACTCT TCTAGAAGTA AATTTGATGC CATTACAAAG 
CAGCCAAGCA GCATTTCTTA CAACTTCACT TCTTCAATTT CCTGGTATGA AAACTCACCA 
AAACCTCAAA TACAAGCCTT CCTTCAGGCA AAAGAAGAAC TGAAGCAACT TAAACTCCCT 
GGGTTCATGT ACAGTGATGT TCCTCTGCTG GCATCCTCAG TACCTTATTT CAGCATGGAA 
GAAGAAGATG GTTCTGAAGA TGGTGTCCAT CTCATTGTCT GCGTGCATGG TTTAGATGGT 
AACAGTGCAG ATCTCCGACT AGTAAAAACG TATATTGAAC TTGGACTGCC TGGGGGAAGA 
GTTGATTTTC TTATGTCTGA GAGAAATCAG AATGATACTT TCGCTGATTT TGATAGCATG 
ACTGATCGTC TTTTGGATGA AATCATACAG TATATTCAGA TATATAGTTT AACAGTCTCA 
AAAATAAGCT TTATTGGACA TTCATTGGGC AATTTAATAA TCCGTTCAGT GCTCACAAGG 
CCACGGTTTA AATATTACCT CAGCAAACTT CATACCTTTC TCTCTCTTTC TGGGCCTCAC 
CTTGGTACAC TGTACAATAG CAGTGCTCTC GTCAATACAG GTCTCTGGTT CATGCAGAAA 
TGGAAAAAAT CAGGTTCTCT TTTGCAACTG ACATGTCGAG ATCATTCAGA CCCTCGCCAA 
ACTTTTTTAT ATAAGCTTAG TAATAAAGCA GGGCTTCATT ATTTCAAGAA TGTTGTGCTG 
GTAGGATCAC TACAGGATCG CTATGTTCCA TATCATTCTG CCCGCATTGA AATGTGCAAA 
ACAGCCTTGA AGGACAAACA GTCAGGACAA ATCTATTCAG AAATGATCCA CAACTTGCTT 
CGCCCAGTTC TGCAAAGCAA AGACTGTAAT TTGATTCGAT ACAATGTCAT CAATGCATTG 
CCCAACACGG CTGATTCACT CATCGGGAGA GCTGCACACA TAGCTGTTCT TGATTCGGAA 
ATATTTTTAG AGAAGTTCTT CCTGGTTGCT GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413666.1
CDS	554..5104
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_029557806.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557806.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTCC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAATT 
CCCCACAGAG TAGAAGCCAG TTTGTTACAT GCAACGGGTA TGACATTAGC CTTTCCAGCT 
TCAGTTCACG ATGCTCTGCT TTGCAGTAAA ACATTTCAAA TCCTATATAA AAATGAAGAA 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAGGAAGATT 
GAAGAAACCC TTGAGGAAAT AAATTTTCTA TTATCCTTGG ATCTGCATTT CACAGACGGA 
GATTATTCGG CAGATGATCT GAACACCTTA CAACTAATAA GTAGTCGAAC ATTGAAGCTG 
CACTTTAGCC TCTCTAGAGG ACTTCATCAT CATGTTAATG TTATGTTTGA TTATTTCCAC 
CTTTCTGTTG TATCTGTTAC AGTCCATGCA TCACTGGTTG CATTACACCA GCCACTTATA 
AGCTTTCCTC GCCCTGTGAA GACCACTTGG TTAAACAGAA ATGCACCAGC ACAGAACAAA 
GATTCTGCAA TTCCCACTCT GGAAAGTGTA GTCTTTGGTA TTAACTACAC AAAACAATTA 
TCACCAGATG GCTGTAGCTT CCTCGTTGCG GAGTCCTTCC TGCATCATGC CTACCGTTTT 
CATTATACAC TTTGTGCCAC TTTGCTACTA GCCTTCAAGG GTTTGCACAA CTACTTCATT 
ACGGTAACAG AAGAGATTCC CTCTTGTCAG AAACTAGAAC TGGATAAAAT GGATGTAGAG 
GCTCGTCTTG CTGAACTGTG TGAAGAAGTT AAAAAAATAG AAAATCCTGA TGAGCTGGCA 
GAACTGATAA ATATGAATCT TGCACAACTT TGCTCACTTC TCATGGCTTT ATGGGGACAG 
TTTCTGGAAG TTATAACACT ACATGAAGAA CTAAGAGTGT TACTAGCACA GGAGCACCAT 
ACTTTACGGG TTCGCAGGTT TTCTGAGGCA TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT CCATGCTCAG AGTCATCTAC AGATGTGCAC CGCTATCAAA 
AATACTTCCT TCTGCAGTTC TCTTCCACCC CTACCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CATTACCTAT AATCTTTGAA GATAGGTATT TAGATTCAGT TATTGAAGAC 
TTAGATGCAC CCTGGACGGG AATTCAGACT CTTCAGATAT CAGAGTCCAG TAGAATGGAT 
AAATATGAGA CTGAAGAAAG CTCTGTAGCA GGACTTTCTA GCCCAGAGTT GAAAGTCAGA 
ACTGCTGGTG CCTCCAGTAT ATGCTGTACA GATGGTGAAA AGCAGCTAAC AAAGTCTCAA 
AAAGGAAAGA ATGAAGAATT AAATAAATCC AAAGTTAAGG TCACTAAGCT CATGAAAACA 
ATGAAACAAG AGAACACAAA AAAGTTAATA AAACAGAACT CTAAAGATTC TGTGATTTTA 
GTAAGCTACA GATGTTTGAA AAGTACACCA TCAAGTGATC TTAGTAAATG CTTTGAAGGC 
AATCCTGCAC ATAGTCAGAA AGAAGGTCTG GATCCCACAA TATGTGGATA TAATTTTGAC 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTCTTTGCC AGCTAATACA 
GAGAGAACTG AACAAAAGTC TCCAGATGTT GAAAATGTGC AACCAGACCA GTTTGATCCC 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCG CATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGACG ATAGTGATAT CACACACATG GAACATAATC TGGCATCAAG AAGTTCATCA 
GATGATTTCC ATGACTGTCA GACAACCCCA TCTTCAGCAG TTAAAACACT TGAAGTAAAT 
TCCACCAATA AAAATCCTTT CGATGGAGAG AAAATAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTAGAT AATTTTCTAT CCACTTTTGA ATCCTTAGAC 
TCTAATGGTA AATCCAAGTC TATAGAAATA ACACTTGAAA AGGAGGCTTT ACAGGAAGCA 
AAATGTCATT CTTTTGGAGA ATCATTAGCT AAATTAAGAA ATAATCTACC TGCTCCTACA 
AAAGAATATC ATGTTGTAGT AAGTGGAGAT ACAATTAAAT TACCAGATAC TAATGCCACA 
TATGCCTCAT CTAGATTTTC AGATTCAGGT GTTGAAAGTG AACCAAGCTC TTTTGCAACA 
CATCCAAACC TGGAAATAGT CTCTGAAACT GGCCAAGGGC AAGGTCCTTA CCATAATGAA 
AGGTTATTTC CTCAGCTTTT GATGAAACCT GATTACAATG TCAAGTTTTC ATTAGGAAGT 
CACTGTACTG AGAGTACAAG TGCTTTAAGT GAAATACAGT CATCTTTGAC ATCAATAAAC 
TCTCTACCTT CTGATGATGA ACTCTCACCT GATGAAAATT CTAAGAAATC TTCTGTGCCT 
GAATGCCATC TAAGTGATAG TAAAACTGTA TTAAATCTAG GAACAATGGA TTTGCCAAAG 
TGTGATGATA CTAAAAAGTC AAGTATTATT TTGCAGCCTC AATGTGTTGT ATTTTCAGGG 
CATTTGGACA ATGAAACTTT AGCAATACAT TCCTTAAATT CAAGCACTGA AGACCCTTTA 
CAACATGTTT TTTCAGATAA AGATACTTCT AGTGATGTGA AAAGTAGTTG TAGCTCCCAG 
CCTAATTTGG ACCCTATATT TAAAGGCTCC CAGAGTCATG ATACATCTAA TAACAACTCT 
GCAGGAACCG TGATCGCAAT ACAGTCAGAA CTGATTTGCT TAGGCACTCC TTGTGTTGTT 
TCGGGTTCTG TTTCTACTAA TTCAGAACTA AGTCAAGAGG GAACTGTGGA AAGAAAAAGT 
AGTGACCCAT TAAGTCTCAA ACAAATGTAT TCAGAAGCCC CTTCAATTGA ACATGAACTT 
CATCTGGGTT CGAGTGACCC TCTTTCAGCC AGTACTGATA TGGTAAAAGA AGGGCTTGTG 
GAAAATTATT TTGGTTCTCA GAGCAGTACA GATATTTCGG ACATACGTGC TGTTAGCTAC 
CACAACTCAG TTAGCCCTCA GAAGGAAACC TGTGAAAAAG GAATCAGTGA CCTTCAGCAG 
GAACAGGGCA AAGATGAGGA GGAGGAAGAA CAGGATCAAC AAATGATTCA AAATGGGTAT 
TATGAAGAAA CAGATTTTTC AGCTTTGGGT GGAACTATAA ATGCTCACTT TCCCAGCAGA 
GATGTGCAAA CAGAGGAAAG GCATCTAAAA TCTGAAGAAG TGAACAGTGA CTATCTGAGG 
GACAGTGTGA ATGTGCCTAC TGTTTGTACT TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA 
CGAGAGTCTC CTTGTAGTGT TAAATACTCT TCTAGAAGTA AATTTGATGC CATTACAAAG 
CAGCCAAGCA GCATTTCTTA CAACTTCACT TCTTCAATTT CCTGGTATGA AAACTCACCA 
AAACCTCAAA TACAAGCCTT CCTTCAGGCA AAAGAAGAAC TGAAGCAACT TAAACTCCCT 
GGGTTCATGT ACAGTGATGT TCCTCTGCTG GCATCCTCAG TACCTTATTT CAGCATGGAA 
GAAGAAGATG GTTCTGAAGA TGGTGTCCAT CTCATTGTCT GCGTGCATGG TTTAGATGGT 
AACAGTGCAG ATCTCCGACT AGTAAAAACG TATATTGAAC TTGGACTGCC TGGGGGAAGA 
GTTGATTTTC TTATGTCTGA GAGAAATCAG AATGATACTT TCGCTGATTT TGATAGCATG 
ACTGATCGTC TTTTGGATGA AATCATACAG TATATTCAGA TATATAGTTT AACAGTCTCA 
AAAATAAGCT TTATTGGACA TTCATTGGGC AATTTAATAA TCCGTTCAGT GCTCACAAGG 
CCACGGTTTA AATATTACCT CAGCAAACTT CATACCTTTC TCTCTCTTTC TGGGCCTCAC 
CTTGGTACAC TGTACAATAG CAGTGCTCTC GTCAATACAG GTCTCTGGTT CATGCAGAAA 
TGGAAAAAAT CAGGTTCTCT TTTGCAACTG ACATGTCGAG ATCATTCAGA CCCTCGCCAA 
ACTTTTTTAT ATAAGCTTAG TAATAAAGCA GGGCTTCATT ATTTCAAGAA TGTTGTGCTG 
GTAGGATCAC TACAGGATCG CTATGTTCCA TATCATTCTG CCCGCATTGA AATGTGCAAA 
ACAGCCTTGA AGGACAAACA GTCAGGACAA ATCTATTCAG AAATGATCCA CAACTTGCTT 
CGCCCAGTTC TGCAAAGCAA AGACTGTAAT TTGATTCGAT ACAATGTCAT CAATGCATTG 
CCCAACACGG CTGATTCACT CATCGGGAGA GCTGCACACA TAGCTGTTCT TGATTCGGAA 
ATATTTTTAG AGAAGTTCTT CCTGGTTGCT GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_017795164.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650653.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X1, mRNA.

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CloneID	ONa38692
Clone ID Related Accession (Same CDS sequence)	XM_017795164.2 , XM_017795164.1
Accession Version	XM_017795164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_017795164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGAC TTAGATGCAC CCTGGACGGG AATTCAGACT 
CTTCAGATAT CAGAGTCCAG TAGAATGGAT AAATATGAGA CTGAAGAAAG CTCTGTAGCA 
GGACTTTCTA GCCCAGAGTT GAAAGTCAGA ACTGCTGGTG CCTCCAGTAT ATGCTGTACA 
GATGGTGAAA AGCAGCTAAC AAAGTCTCAA AAAGGAAAGA ATGAAGAATT AAATAAATCC 
AAAGTTAAGG TCACTAAGCT CATGAAAACA ATGAAACAAG AGAACACAAA AAAGTTAATA 
AAACAGAACT CTAAAGATTC TGTGATTTTA GTAAGCTACA GATGTTTGAA AAGTACACCA 
TCAAGTGATC TTAGTAAATG CTTTGAAGGC AATCCTGCAC ATAGTCAGAA AGAAGGTCTG 
GATCCCACAA TATGTGGATA TAATTTTGAC CCAAAGACCT ACATCAGACA GACAAGTCAA 
AAGGAAGCTA GCTCTTTGCC AGCTAATACA GAGAGAACTG AACAAAAGTC TCCAGATGTT 
GAAAATGTGC AACCAGACCA GTTTGATCCC TTGAACTCTG GCAGCCTAAA TCTTTGTGCG 
CATTTGTCCA TTTCAGGTAA ACTTGGTATC TCCCAGGACG ATAGTGATAT CACACACATG 
GAACATAATC TGGCATCAAG AAGTTCATCA GATGATTTCC ATGACTGTCA GACAACCCCA 
TCTTCAGCAG TTAAAACACT TGAAGTAAAT TCCACCAATA AAAATCCTTT CGATGGAGAG 
AAAATAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTAGAT 
AATTTTCTAT CCACTTTTGA ATCCTTAGAC TCTAATGGTA AATCCAAGTC TATAGAAATA 
ACACTTGAAA AGGAGGCTTT ACAGGAAGCA AAATGTCATT CTTTTGGAGA ATCATTAGCT 
AAATTAAGAA ATAATCTACC TGCTCCTACA AAAGAATATC ATGTTGTAGT AAGTGGAGAT 
ACAATTAAAT TACCAGATAC TAATGCCACA TATGCCTCAT CTAGATTTTC AGATTCAGGT 
GTTGAAAGTG AACCAAGCTC TTTTGCAACA CATCCAAACC TGGAAATAGT CTCTGAAACT 
GGCCAAGGGC AAGGTCCTTA CCATAATGAA AGGTTATTTC CTCAGCTTTT GATGAAACCT 
GATTACAATG TCAAGTTTTC ATTAGGAAGT CACTGTACTG AGAGTACAAG TGCTTTAAGT 
GAAATACAGT CATCTTTGAC ATCAATAAAC TCTCTACCTT CTGATGATGA ACTCTCACCT 
GATGAAAATT CTAAGAAATC TTCTGTGCCT GAATGCCATC TAAGTGATAG TAAAACTGTA 
TTAAATCTAG GAACAATGGA TTTGCCAAAG TGTGATGATA CTAAAAAGTC AAGTATTATT 
TTGCAGCCTC AATGTGTTGT ATTTTCAGGG CATTTGGACA ATGAAACTTT AGCAATACAT 
TCCTTAAATT CAAGCACTGA AGACCCTTTA CAACATGTTT TTTCAGATAA AGATACTTCT 
AGTGATGTGA AAAGTAGTTG TAGCTCCCAG CCTAATTTGG ACCCTATATT TAAAGGCTCC 
CAGAGTCATG ATACATCTAA TAACAACTCT GCAGGAACCG TGATCGCAAT ACAGTCAGAA 
CTGATTTGCT TAGGCACTCC TTGTGTTGTT TCGGGTTCTG TTTCTACTAA TTCAGAACTA 
AGTCAAGAGG GAACTGTGGA AAGAAAAAGT AGTGACCCAT TAAGTCTCAA ACAAATGTAT 
TCAGAAGCCC CTTCAATTGA ACATGAACTT CATCTGGGTT CGAGTGACCC TCTTTCAGCC 
AGTACTGATA TGGTAAAAGA AGGGCTTGTG GAAAATTATT TTGGTTCTCA GAGCAGTACA 
GATATTTCGG ACATACGTGC TGTTAGCTAC CACAACTCAG TTAGCCCTCA GAAGGAAACC 
TGTGAAAAAG GAATCAGTGA CCTTCAGCAG GAACAGGGCA AAGATGAGGA GGAGGAAGAA 
CAGGATCAAC AAATGATTCA AAATGGGTAT TATGAAGAAA CAGATTTTTC AGCTTTGGGT 
GGAACTATAA ATGCTCACTT TCCCAGCAGA GATGTGCAAA CAGAGGAAAG GCATCTAAAA 
TCTGAAGAAG TGAACAGTGA CTATCTGAGG GACAGTGTGA ATGTGCCTAC TGTTTGTACT 
TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA CGAGAGTCTC CTTGTAGTGT TAAATACTCT 
TCTAGAAGTA AATTTGATGC CATTACAAAG CAGCCAAGCA GCATTTCTTA CAACTTCACT 
TCTTCAATTT CCTGGTATGA AAACTCACCA AAACCTCAAA TACAAGCCTT CCTTCAGGCA 
AAAGAAGAAC TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGTGATGT TCCTCTGCTG 
GCATCCTCAG TACCTTATTT CAGCATGGAA GAAGAAGATG GTTCTGAAGA TGGTGTCCAT 
CTCATTGTCT GCGTGCATGG TTTAGATGGT AACAGTGCAG ATCTCCGACT AGTAAAAACG 
TATATTGAAC TTGGACTGCC TGGGGGAAGA GTTGATTTTC TTATGTCTGA GAGAAATCAG 
AATGATACTT TCGCTGATTT TGATAGCATG ACTGATCGTC TTTTGGATGA AATCATACAG 
TATATTCAGA TATATAGTTT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCATTGGGC 
AATTTAATAA TCCGTTCAGT GCTCACAAGG CCACGGTTTA AATATTACCT CAGCAAACTT 
CATACCTTTC TCTCTCTTTC TGGGCCTCAC CTTGGTACAC TGTACAATAG CAGTGCTCTC 
GTCAATACAG GTCTCTGGTT CATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTGCAACTG 
ACATGTCGAG ATCATTCAGA CCCTCGCCAA ACTTTTTTAT ATAAGCTTAG TAATAAAGCA 
GGGCTTCATT ATTTCAAGAA TGTTGTGCTG GTAGGATCAC TACAGGATCG CTATGTTCCA 
TATCATTCTG CCCGCATTGA AATGTGCAAA ACAGCCTTGA AGGACAAACA GTCAGGACAA 
ATCTATTCAG AAATGATCCA CAACTTGCTT CGCCCAGTTC TGCAAAGCAA AGACTGTAAT 
TTGATTCGAT ACAATGTCAT CAATGCATTG CCCAACACGG CTGATTCACT CATCGGGAGA 
GCTGCACACA TAGCTGTTCT TGATTCGGAA ATATTTTTAG AGAAGTTCTT CCTGGTTGCT 
GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650653.1
CDS	249..4889
Translation	MVLTRVGPSSSWTQSSSKGQTRGEAEPAKSFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_017795164.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795164.2

ORF Insert Sequence:

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGAC TTAGATGCAC CCTGGACGGG AATTCAGACT 
CTTCAGATAT CAGAGTCCAG TAGAATGGAT AAATATGAGA CTGAAGAAAG CTCTGTAGCA 
GGACTTTCTA GCCCAGAGTT GAAAGTCAGA ACTGCTGGTG CCTCCAGTAT ATGCTGTACA 
GATGGTGAAA AGCAGCTAAC AAAGTCTCAA AAAGGAAAGA ATGAAGAATT AAATAAATCC 
AAAGTTAAGG TCACTAAGCT CATGAAAACA ATGAAACAAG AGAACACAAA AAAGTTAATA 
AAACAGAACT CTAAAGATTC TGTGATTTTA GTAAGCTACA GATGTTTGAA AAGTACACCA 
TCAAGTGATC TTAGTAAATG CTTTGAAGGC AATCCTGCAC ATAGTCAGAA AGAAGGTCTG 
GATCCCACAA TATGTGGATA TAATTTTGAC CCAAAGACCT ACATCAGACA GACAAGTCAA 
AAGGAAGCTA GCTCTTTGCC AGCTAATACA GAGAGAACTG AACAAAAGTC TCCAGATGTT 
GAAAATGTGC AACCAGACCA GTTTGATCCC TTGAACTCTG GCAGCCTAAA TCTTTGTGCG 
CATTTGTCCA TTTCAGGTAA ACTTGGTATC TCCCAGGACG ATAGTGATAT CACACACATG 
GAACATAATC TGGCATCAAG AAGTTCATCA GATGATTTCC ATGACTGTCA GACAACCCCA 
TCTTCAGCAG TTAAAACACT TGAAGTAAAT TCCACCAATA AAAATCCTTT CGATGGAGAG 
AAAATAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTAGAT 
AATTTTCTAT CCACTTTTGA ATCCTTAGAC TCTAATGGTA AATCCAAGTC TATAGAAATA 
ACACTTGAAA AGGAGGCTTT ACAGGAAGCA AAATGTCATT CTTTTGGAGA ATCATTAGCT 
AAATTAAGAA ATAATCTACC TGCTCCTACA AAAGAATATC ATGTTGTAGT AAGTGGAGAT 
ACAATTAAAT TACCAGATAC TAATGCCACA TATGCCTCAT CTAGATTTTC AGATTCAGGT 
GTTGAAAGTG AACCAAGCTC TTTTGCAACA CATCCAAACC TGGAAATAGT CTCTGAAACT 
GGCCAAGGGC AAGGTCCTTA CCATAATGAA AGGTTATTTC CTCAGCTTTT GATGAAACCT 
GATTACAATG TCAAGTTTTC ATTAGGAAGT CACTGTACTG AGAGTACAAG TGCTTTAAGT 
GAAATACAGT CATCTTTGAC ATCAATAAAC TCTCTACCTT CTGATGATGA ACTCTCACCT 
GATGAAAATT CTAAGAAATC TTCTGTGCCT GAATGCCATC TAAGTGATAG TAAAACTGTA 
TTAAATCTAG GAACAATGGA TTTGCCAAAG TGTGATGATA CTAAAAAGTC AAGTATTATT 
TTGCAGCCTC AATGTGTTGT ATTTTCAGGG CATTTGGACA ATGAAACTTT AGCAATACAT 
TCCTTAAATT CAAGCACTGA AGACCCTTTA CAACATGTTT TTTCAGATAA AGATACTTCT 
AGTGATGTGA AAAGTAGTTG TAGCTCCCAG CCTAATTTGG ACCCTATATT TAAAGGCTCC 
CAGAGTCATG ATACATCTAA TAACAACTCT GCAGGAACCG TGATCGCAAT ACAGTCAGAA 
CTGATTTGCT TAGGCACTCC TTGTGTTGTT TCGGGTTCTG TTTCTACTAA TTCAGAACTA 
AGTCAAGAGG GAACTGTGGA AAGAAAAAGT AGTGACCCAT TAAGTCTCAA ACAAATGTAT 
TCAGAAGCCC CTTCAATTGA ACATGAACTT CATCTGGGTT CGAGTGACCC TCTTTCAGCC 
AGTACTGATA TGGTAAAAGA AGGGCTTGTG GAAAATTATT TTGGTTCTCA GAGCAGTACA 
GATATTTCGG ACATACGTGC TGTTAGCTAC CACAACTCAG TTAGCCCTCA GAAGGAAACC 
TGTGAAAAAG GAATCAGTGA CCTTCAGCAG GAACAGGGCA AAGATGAGGA GGAGGAAGAA 
CAGGATCAAC AAATGATTCA AAATGGGTAT TATGAAGAAA CAGATTTTTC AGCTTTGGGT 
GGAACTATAA ATGCTCACTT TCCCAGCAGA GATGTGCAAA CAGAGGAAAG GCATCTAAAA 
TCTGAAGAAG TGAACAGTGA CTATCTGAGG GACAGTGTGA ATGTGCCTAC TGTTTGTACT 
TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA CGAGAGTCTC CTTGTAGTGT TAAATACTCT 
TCTAGAAGTA AATTTGATGC CATTACAAAG CAGCCAAGCA GCATTTCTTA CAACTTCACT 
TCTTCAATTT CCTGGTATGA AAACTCACCA AAACCTCAAA TACAAGCCTT CCTTCAGGCA 
AAAGAAGAAC TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGTGATGT TCCTCTGCTG 
GCATCCTCAG TACCTTATTT CAGCATGGAA GAAGAAGATG GTTCTGAAGA TGGTGTCCAT 
CTCATTGTCT GCGTGCATGG TTTAGATGGT AACAGTGCAG ATCTCCGACT AGTAAAAACG 
TATATTGAAC TTGGACTGCC TGGGGGAAGA GTTGATTTTC TTATGTCTGA GAGAAATCAG 
AATGATACTT TCGCTGATTT TGATAGCATG ACTGATCGTC TTTTGGATGA AATCATACAG 
TATATTCAGA TATATAGTTT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCATTGGGC 
AATTTAATAA TCCGTTCAGT GCTCACAAGG CCACGGTTTA AATATTACCT CAGCAAACTT 
CATACCTTTC TCTCTCTTTC TGGGCCTCAC CTTGGTACAC TGTACAATAG CAGTGCTCTC 
GTCAATACAG GTCTCTGGTT CATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTGCAACTG 
ACATGTCGAG ATCATTCAGA CCCTCGCCAA ACTTTTTTAT ATAAGCTTAG TAATAAAGCA 
GGGCTTCATT ATTTCAAGAA TGTTGTGCTG GTAGGATCAC TACAGGATCG CTATGTTCCA 
TATCATTCTG CCCGCATTGA AATGTGCAAA ACAGCCTTGA AGGACAAACA GTCAGGACAA 
ATCTATTCAG AAATGATCCA CAACTTGCTT CGCCCAGTTC TGCAAAGCAA AGACTGTAAT 
TTGATTCGAT ACAATGTCAT CAATGCATTG CCCAACACGG CTGATTCACT CATCGGGAGA 
GCTGCACACA TAGCTGTTCT TGATTCGGAA ATATTTTTAG AGAAGTTCTT CCTGGTTGCT 
GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_017795164.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650653.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X1, mRNA.

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CloneID	ONa38692
Clone ID Related Accession (Same CDS sequence)	XM_017795164.2 , XM_017795164.1
Accession Version	XM_017795164.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGAC TTAGATGCAC CCTGGACGGG AATTCAGACT 
CTTCAGATAT CAGAGTCCAG TAGAATGGAT AAATATGAGA CTGAAGAAAG CTCTGTAGCA 
GGACTTTCTA GCCCAGAGTT GAAAGTCAGA ACTGCTGGTG CCTCCAGTAT ATGCTGTACA 
GATGGTGAAA AGCAGCTAAC AAAGTCTCAA AAAGGAAAGA ATGAAGAATT AAATAAATCC 
AAAGTTAAGG TCACTAAGCT CATGAAAACA ATGAAACAAG AGAACACAAA AAAGTTAATA 
AAACAGAACT CTAAAGATTC TGTGATTTTA GTAAGCTACA GATGTTTGAA AAGTACACCA 
TCAAGTGATC TTAGTAAATG CTTTGAAGGC AATCCTGCAC ATAGTCAGAA AGAAGGTCTG 
GATCCCACAA TATGTGGATA TAATTTTGAC CCAAAGACCT ACATCAGACA GACAAGTCAA 
AAGGAAGCTA GCTCTTTGCC AGCTAATACA GAGAGAACTG AACAAAAGTC TCCAGATGTT 
GAAAATGTGC AACCAGACCA GTTTGATCCC TTGAACTCTG GCAGCCTAAA TCTTTGTGCG 
CATTTGTCCA TTTCAGGTAA ACTTGGTATC TCCCAGGACG ATAGTGATAT CACACACATG 
GAACATAATC TGGCATCAAG AAGTTCATCA GATGATTTCC ATGACTGTCA GACAACCCCA 
TCTTCAGCAG TTAAAACACT TGAAGTAAAT TCCACCAATA AAAATCCTTT CGATGGAGAG 
AAAATAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTAGAT 
AATTTTCTAT CCACTTTTGA ATCCTTAGAC TCTAATGGTA AATCCAAGTC TATAGAAATA 
ACACTTGAAA AGGAGGCTTT ACAGGAAGCA AAATGTCATT CTTTTGGAGA ATCATTAGCT 
AAATTAAGAA ATAATCTACC TGCTCCTACA AAAGAATATC ATGTTGTAGT AAGTGGAGAT 
ACAATTAAAT TACCAGATAC TAATGCCACA TATGCCTCAT CTAGATTTTC AGATTCAGGT 
GTTGAAAGTG AACCAAGCTC TTTTGCAACA CATCCAAACC TGGAAATAGT CTCTGAAACT 
GGCCAAGGGC AAGGTCCTTA CCATAATGAA AGGTTATTTC CTCAGCTTTT GATGAAACCT 
GATTACAATG TCAAGTTTTC ATTAGGAAGT CACTGTACTG AGAGTACAAG TGCTTTAAGT 
GAAATACAGT CATCTTTGAC ATCAATAAAC TCTCTACCTT CTGATGATGA ACTCTCACCT 
GATGAAAATT CTAAGAAATC TTCTGTGCCT GAATGCCATC TAAGTGATAG TAAAACTGTA 
TTAAATCTAG GAACAATGGA TTTGCCAAAG TGTGATGATA CTAAAAAGTC AAGTATTATT 
TTGCAGCCTC AATGTGTTGT ATTTTCAGGG CATTTGGACA ATGAAACTTT AGCAATACAT 
TCCTTAAATT CAAGCACTGA AGACCCTTTA CAACATGTTT TTTCAGATAA AGATACTTCT 
AGTGATGTGA AAAGTAGTTG TAGCTCCCAG CCTAATTTGG ACCCTATATT TAAAGGCTCC 
CAGAGTCATG ATACATCTAA TAACAACTCT GCAGGAACCG TGATCGCAAT ACAGTCAGAA 
CTGATTTGCT TAGGCACTCC TTGTGTTGTT TCGGGTTCTG TTTCTACTAA TTCAGAACTA 
AGTCAAGAGG GAACTGTGGA AAGAAAAAGT AGTGACCCAT TAAGTCTCAA ACAAATGTAT 
TCAGAAGCCC CTTCAATTGA ACATGAACTT CATCTGGGTT CGAGTGACCC TCTTTCAGCC 
AGTACTGATA TGGTAAAAGA AGGGCTTGTG GAAAATTATT TTGGTTCTCA GAGCAGTACA 
GATATTTCGG ACATACGTGC TGTTAGCTAC CACAACTCAG TTAGCCCTCA GAAGGAAACC 
TGTGAAAAAG GAATCAGTGA CCTTCAGCAG GAACAGGGCA AAGATGAGGA GGAGGAAGAA 
CAGGATCAAC AAATGATTCA AAATGGGTAT TATGAAGAAA CAGATTTTTC AGCTTTGGGT 
GGAACTATAA ATGCTCACTT TCCCAGCAGA GATGTGCAAA CAGAGGAAAG GCATCTAAAA 
TCTGAAGAAG TGAACAGTGA CTATCTGAGG GACAGTGTGA ATGTGCCTAC TGTTTGTACT 
TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA CGAGAGTCTC CTTGTAGTGT TAAATACTCT 
TCTAGAAGTA AATTTGATGC CATTACAAAG CAGCCAAGCA GCATTTCTTA CAACTTCACT 
TCTTCAATTT CCTGGTATGA AAACTCACCA AAACCTCAAA TACAAGCCTT CCTTCAGGCA 
AAAGAAGAAC TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGTGATGT TCCTCTGCTG 
GCATCCTCAG TACCTTATTT CAGCATGGAA GAAGAAGATG GTTCTGAAGA TGGTGTCCAT 
CTCATTGTCT GCGTGCATGG TTTAGATGGT AACAGTGCAG ATCTCCGACT AGTAAAAACG 
TATATTGAAC TTGGACTGCC TGGGGGAAGA GTTGATTTTC TTATGTCTGA GAGAAATCAG 
AATGATACTT TCGCTGATTT TGATAGCATG ACTGATCGTC TTTTGGATGA AATCATACAG 
TATATTCAGA TATATAGTTT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCATTGGGC 
AATTTAATAA TCCGTTCAGT GCTCACAAGG CCACGGTTTA AATATTACCT CAGCAAACTT 
CATACCTTTC TCTCTCTTTC TGGGCCTCAC CTTGGTACAC TGTACAATAG CAGTGCTCTC 
GTCAATACAG GTCTCTGGTT CATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTGCAACTG 
ACATGTCGAG ATCATTCAGA CCCTCGCCAA ACTTTTTTAT ATAAGCTTAG TAATAAAGCA 
GGGCTTCATT ATTTCAAGAA TGTTGTGCTG GTAGGATCAC TACAGGATCG CTATGTTCCA 
TATCATTCTG CCCGCATTGA AATGTGCAAA ACAGCCTTGA AGGACAAACA GTCAGGACAA 
ATCTATTCAG AAATGATCCA CAACTTGCTT CGCCCAGTTC TGCAAAGCAA AGACTGTAAT 
TTGATTCGAT ACAATGTCAT CAATGCATTG CCCAACACGG CTGATTCACT CATCGGGAGA 
GCTGCACACA TAGCTGTTCT TGATTCGGAA ATATTTTTAG AGAAGTTCTT CCTGGTTGCT 
GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650653.1
CDS	108..4748
Translation	MVLTRVGPSSSWTQSSSKGQTRGEAEPAKSFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELAKANMQVLYERLLRRKQPRTQNDTCVDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_017795164.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795164.1

ORF Insert Sequence:

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGCCAAG 
GCCAACATGC AGGTCCTTTA TGAACGCCTC CTCAGAAGAA AACAGCCAAG AACCCAGAAT 
GACACCTGTG TAGATAAAAT GGATGTAGAG GCTCGTCTTG CTGAACTGTG TGAAGAAGTT 
AAAAAAATAG AAAATCCTGA TGAGCTGGCA GAACTGATAA ATATGAATCT TGCACAACTT 
TGCTCACTTC TCATGGCTTT ATGGGGACAG TTTCTGGAAG TTATAACACT ACATGAAGAA 
CTAAGAGTGT TACTAGCACA GGAGCACCAT ACTTTACGGG TTCGCAGGTT TTCTGAGGCA 
TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT CCATGCTCAG 
AGTCATCTAC AGATGTGCAC CGCTATCAAA AATACTTCCT TCTGCAGTTC TCTTCCACCC 
CTACCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CATTACCTAT AATCTTTGAA 
GATAGGTATT TAGATTCAGT TATTGAAGAC TTAGATGCAC CCTGGACGGG AATTCAGACT 
CTTCAGATAT CAGAGTCCAG TAGAATGGAT AAATATGAGA CTGAAGAAAG CTCTGTAGCA 
GGACTTTCTA GCCCAGAGTT GAAAGTCAGA ACTGCTGGTG CCTCCAGTAT ATGCTGTACA 
GATGGTGAAA AGCAGCTAAC AAAGTCTCAA AAAGGAAAGA ATGAAGAATT AAATAAATCC 
AAAGTTAAGG TCACTAAGCT CATGAAAACA ATGAAACAAG AGAACACAAA AAAGTTAATA 
AAACAGAACT CTAAAGATTC TGTGATTTTA GTAAGCTACA GATGTTTGAA AAGTACACCA 
TCAAGTGATC TTAGTAAATG CTTTGAAGGC AATCCTGCAC ATAGTCAGAA AGAAGGTCTG 
GATCCCACAA TATGTGGATA TAATTTTGAC CCAAAGACCT ACATCAGACA GACAAGTCAA 
AAGGAAGCTA GCTCTTTGCC AGCTAATACA GAGAGAACTG AACAAAAGTC TCCAGATGTT 
GAAAATGTGC AACCAGACCA GTTTGATCCC TTGAACTCTG GCAGCCTAAA TCTTTGTGCG 
CATTTGTCCA TTTCAGGTAA ACTTGGTATC TCCCAGGACG ATAGTGATAT CACACACATG 
GAACATAATC TGGCATCAAG AAGTTCATCA GATGATTTCC ATGACTGTCA GACAACCCCA 
TCTTCAGCAG TTAAAACACT TGAAGTAAAT TCCACCAATA AAAATCCTTT CGATGGAGAG 
AAAATAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTAGAT 
AATTTTCTAT CCACTTTTGA ATCCTTAGAC TCTAATGGTA AATCCAAGTC TATAGAAATA 
ACACTTGAAA AGGAGGCTTT ACAGGAAGCA AAATGTCATT CTTTTGGAGA ATCATTAGCT 
AAATTAAGAA ATAATCTACC TGCTCCTACA AAAGAATATC ATGTTGTAGT AAGTGGAGAT 
ACAATTAAAT TACCAGATAC TAATGCCACA TATGCCTCAT CTAGATTTTC AGATTCAGGT 
GTTGAAAGTG AACCAAGCTC TTTTGCAACA CATCCAAACC TGGAAATAGT CTCTGAAACT 
GGCCAAGGGC AAGGTCCTTA CCATAATGAA AGGTTATTTC CTCAGCTTTT GATGAAACCT 
GATTACAATG TCAAGTTTTC ATTAGGAAGT CACTGTACTG AGAGTACAAG TGCTTTAAGT 
GAAATACAGT CATCTTTGAC ATCAATAAAC TCTCTACCTT CTGATGATGA ACTCTCACCT 
GATGAAAATT CTAAGAAATC TTCTGTGCCT GAATGCCATC TAAGTGATAG TAAAACTGTA 
TTAAATCTAG GAACAATGGA TTTGCCAAAG TGTGATGATA CTAAAAAGTC AAGTATTATT 
TTGCAGCCTC AATGTGTTGT ATTTTCAGGG CATTTGGACA ATGAAACTTT AGCAATACAT 
TCCTTAAATT CAAGCACTGA AGACCCTTTA CAACATGTTT TTTCAGATAA AGATACTTCT 
AGTGATGTGA AAAGTAGTTG TAGCTCCCAG CCTAATTTGG ACCCTATATT TAAAGGCTCC 
CAGAGTCATG ATACATCTAA TAACAACTCT GCAGGAACCG TGATCGCAAT ACAGTCAGAA 
CTGATTTGCT TAGGCACTCC TTGTGTTGTT TCGGGTTCTG TTTCTACTAA TTCAGAACTA 
AGTCAAGAGG GAACTGTGGA AAGAAAAAGT AGTGACCCAT TAAGTCTCAA ACAAATGTAT 
TCAGAAGCCC CTTCAATTGA ACATGAACTT CATCTGGGTT CGAGTGACCC TCTTTCAGCC 
AGTACTGATA TGGTAAAAGA AGGGCTTGTG GAAAATTATT TTGGTTCTCA GAGCAGTACA 
GATATTTCGG ACATACGTGC TGTTAGCTAC CACAACTCAG TTAGCCCTCA GAAGGAAACC 
TGTGAAAAAG GAATCAGTGA CCTTCAGCAG GAACAGGGCA AAGATGAGGA GGAGGAAGAA 
CAGGATCAAC AAATGATTCA AAATGGGTAT TATGAAGAAA CAGATTTTTC AGCTTTGGGT 
GGAACTATAA ATGCTCACTT TCCCAGCAGA GATGTGCAAA CAGAGGAAAG GCATCTAAAA 
TCTGAAGAAG TGAACAGTGA CTATCTGAGG GACAGTGTGA ATGTGCCTAC TGTTTGTACT 
TCGGGTTGTT TGTCTTTCCC TTCTGCTCCA CGAGAGTCTC CTTGTAGTGT TAAATACTCT 
TCTAGAAGTA AATTTGATGC CATTACAAAG CAGCCAAGCA GCATTTCTTA CAACTTCACT 
TCTTCAATTT CCTGGTATGA AAACTCACCA AAACCTCAAA TACAAGCCTT CCTTCAGGCA 
AAAGAAGAAC TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGTGATGT TCCTCTGCTG 
GCATCCTCAG TACCTTATTT CAGCATGGAA GAAGAAGATG GTTCTGAAGA TGGTGTCCAT 
CTCATTGTCT GCGTGCATGG TTTAGATGGT AACAGTGCAG ATCTCCGACT AGTAAAAACG 
TATATTGAAC TTGGACTGCC TGGGGGAAGA GTTGATTTTC TTATGTCTGA GAGAAATCAG 
AATGATACTT TCGCTGATTT TGATAGCATG ACTGATCGTC TTTTGGATGA AATCATACAG 
TATATTCAGA TATATAGTTT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCATTGGGC 
AATTTAATAA TCCGTTCAGT GCTCACAAGG CCACGGTTTA AATATTACCT CAGCAAACTT 
CATACCTTTC TCTCTCTTTC TGGGCCTCAC CTTGGTACAC TGTACAATAG CAGTGCTCTC 
GTCAATACAG GTCTCTGGTT CATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTGCAACTG 
ACATGTCGAG ATCATTCAGA CCCTCGCCAA ACTTTTTTAT ATAAGCTTAG TAATAAAGCA 
GGGCTTCATT ATTTCAAGAA TGTTGTGCTG GTAGGATCAC TACAGGATCG CTATGTTCCA 
TATCATTCTG CCCGCATTGA AATGTGCAAA ACAGCCTTGA AGGACAAACA GTCAGGACAA 
ATCTATTCAG AAATGATCCA CAACTTGCTT CGCCCAGTTC TGCAAAGCAA AGACTGTAAT 
TTGATTCGAT ACAATGTCAT CAATGCATTG CCCAACACGG CTGATTCACT CATCGGGAGA 
GCTGCACACA TAGCTGTTCT TGATTCGGAA ATATTTTTAG AGAAGTTCTT CCTGGTTGCT 
GCCCTCAAAT ATTTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_008856282.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008854504.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X2, mRNA.

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CloneID	ONa38693
Clone ID Related Accession (Same CDS sequence)	XM_008856282.1 , XM_008856282.2
Accession Version	XM_008856282.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGATAAA 
ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG ACTTAGATGC ACCCTGGACG GGAATTCAGA CTCTTCAGAT ATCAGAGTCC 
AGTAGAATGG ATAAATATGA GACTGAAGAA AGCTCTGTAG CAGGACTTTC TAGCCCAGAG 
TTGAAAGTCA GAACTGCTGG TGCCTCCAGT ATATGCTGTA CAGATGGTGA AAAGCAGCTA 
ACAAAGTCTC AAAAAGGAAA GAATGAAGAA TTAAATAAAT CCAAAGTTAA GGTCACTAAG 
CTCATGAAAA CAATGAAACA AGAGAACACA AAAAAGTTAA TAAAACAGAA CTCTAAAGAT 
TCTGTGATTT TAGTAAGCTA CAGATGTTTG AAAAGTACAC CATCAAGTGA TCTTAGTAAA 
TGCTTTGAAG GCAATCCTGC ACATAGTCAG AAAGAAGGTC TGGATCCCAC AATATGTGGA 
TATAATTTTG ACCCAAAGAC CTACATCAGA CAGACAAGTC AAAAGGAAGC TAGCTCTTTG 
CCAGCTAATA CAGAGAGAAC TGAACAAAAG TCTCCAGATG TTGAAAATGT GCAACCAGAC 
CAGTTTGATC CCTTGAACTC TGGCAGCCTA AATCTTTGTG CGCATTTGTC CATTTCAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008854504.1
CDS	108..4670
Translation	MVLTRVGPSSSWTQSSSKGQTRGEAEPAKSFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_008856282.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008856282.1

ORF Insert Sequence:

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGATAAA 
ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG ACTTAGATGC ACCCTGGACG GGAATTCAGA CTCTTCAGAT ATCAGAGTCC 
AGTAGAATGG ATAAATATGA GACTGAAGAA AGCTCTGTAG CAGGACTTTC TAGCCCAGAG 
TTGAAAGTCA GAACTGCTGG TGCCTCCAGT ATATGCTGTA CAGATGGTGA AAAGCAGCTA 
ACAAAGTCTC AAAAAGGAAA GAATGAAGAA TTAAATAAAT CCAAAGTTAA GGTCACTAAG 
CTCATGAAAA CAATGAAACA AGAGAACACA AAAAAGTTAA TAAAACAGAA CTCTAAAGAT 
TCTGTGATTT TAGTAAGCTA CAGATGTTTG AAAAGTACAC CATCAAGTGA TCTTAGTAAA 
TGCTTTGAAG GCAATCCTGC ACATAGTCAG AAAGAAGGTC TGGATCCCAC AATATGTGGA 
TATAATTTTG ACCCAAAGAC CTACATCAGA CAGACAAGTC AAAAGGAAGC TAGCTCTTTG 
CCAGCTAATA CAGAGAGAAC TGAACAAAAG TCTCCAGATG TTGAAAATGT GCAACCAGAC 
CAGTTTGATC CCTTGAACTC TGGCAGCCTA AATCTTTGTG CGCATTTGTC CATTTCAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam135a ( XM_008856282.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008854504.1 Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X5, mRNA.

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CloneID	ONa38693
Clone ID Related Accession (Same CDS sequence)	XM_008856282.1 , XM_008856282.2
Accession Version	XM_008856282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	protein FAM135A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358101.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008856282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGATAAA 
ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG ACTTAGATGC ACCCTGGACG GGAATTCAGA CTCTTCAGAT ATCAGAGTCC 
AGTAGAATGG ATAAATATGA GACTGAAGAA AGCTCTGTAG CAGGACTTTC TAGCCCAGAG 
TTGAAAGTCA GAACTGCTGG TGCCTCCAGT ATATGCTGTA CAGATGGTGA AAAGCAGCTA 
ACAAAGTCTC AAAAAGGAAA GAATGAAGAA TTAAATAAAT CCAAAGTTAA GGTCACTAAG 
CTCATGAAAA CAATGAAACA AGAGAACACA AAAAAGTTAA TAAAACAGAA CTCTAAAGAT 
TCTGTGATTT TAGTAAGCTA CAGATGTTTG AAAAGTACAC CATCAAGTGA TCTTAGTAAA 
TGCTTTGAAG GCAATCCTGC ACATAGTCAG AAAGAAGGTC TGGATCCCAC AATATGTGGA 
TATAATTTTG ACCCAAAGAC CTACATCAGA CAGACAAGTC AAAAGGAAGC TAGCTCTTTG 
CCAGCTAATA CAGAGAGAAC TGAACAAAAG TCTCCAGATG TTGAAAATGT GCAACCAGAC 
CAGTTTGATC CCTTGAACTC TGGCAGCCTA AATCTTTGTG CGCATTTGTC CATTTCAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008854504.1
CDS	249..4811
Translation	MVLTRVGPSSSWTQSSSKGQTRGEAEPAKSFYQIRASMKIPPRIPHRVEASLLHATGMTLAFPASVHDALLCSKTFQILYKNEEVVLNDVMIFKVKMLLDERKIEETLEEINFLLSLDLHFTDGDYSADDLNTLQLISSRTLKLHFSLSRGLHHHVNVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSAIPTLESVVFGINYTKQLSPDGCSFLVAESFLHHAYRFHYTLCATLLLAFKGLHNYFITVTEEIPSCQKLELDKMDVEARLAELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRVLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVIEDLDAPWTGIQTLQISESSRMDKYETEESSVAGLSSPELKVRTAGASSICCTDGEKQLTKSQKGKNEELNKSKVKVTKLMKTMKQENTKKLIKQNSKDSVILVSYRCLKSTPSSDLSKCFEGNPAHSQKEGLDPTICGYNFDPKTYIRQTSQKEASSLPANTERTEQKSPDVENVQPDQFDPLNSGSLNLCAHLSISGKLGISQDDSDITHMEHNLASRSSSDDFHDCQTTPSSAVKTLEVNSTNKNPFDGEKITVKIGPWTELQQDEIFVDNFLSTFESLDSNGKSKSIEITLEKEALQEAKCHSFGESLAKLRNNLPAPTKEYHVVVSGDTIKLPDTNATYASSRFSDSGVESEPSSFATHPNLEIVSETGQGQGPYHNERLFPQLLMKPDYNVKFSLGSHCTESTSALSEIQSSLTSINSLPSDDELSPDENSKKSSVPECHLSDSKTVLNLGTMDLPKCDDTKKSSIILQPQCVVFSGHLDNETLAIHSLNSSTEDPLQHVFSDKDTSSDVKSSCSSQPNLDPIFKGSQSHDTSNNNSAGTVIAIQSELICLGTPCVVSGSVSTNSELSQEGTVERKSSDPLSLKQMYSEAPSIEHELHLGSSDPLSASTDMVKEGLVENYFGSQSSTDISDIRAVSYHNSVSPQKETCEKGISDLQQEQGKDEEEEEQDQQMIQNGYYEETDFSALGGTINAHFPSRDVQTEERHLKSEEVNSDYLRDSVNVPTVCTSGCLSFPSAPRESPCSVKYSSRSKFDAITKQPSSISYNFTSSISWYENSPKPQIQAFLQAKEELKQLKLPGFMYSDVPLLASSVPYFSMEEEDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRVDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLIRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_008856282.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili family with sequence similarity 135 member A (Fam135a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008856282.2

ORF Insert Sequence:

ATGGTGCTGA CCCGGGTAGG GCCGTCTTCC AGCTGGACGC AGAGCTCCTC CAAAGGACAG 
ACGAGGGGAG AGGCCGAGCC GGCTAAGAGT TTTTACCAGA TTCGTGCATC TATGAAAATT 
CCACCAAGAA TTCCCCACAG AGTAGAAGCC AGTTTGTTAC ATGCAACGGG TATGACATTA 
GCCTTTCCAG CTTCAGTTCA CGATGCTCTG CTTTGCAGTA AAACATTTCA AATCCTATAT 
AAAAATGAAG AAGTTGTTTT AAATGATGTC ATGATCTTCA AAGTTAAAAT GTTGTTGGAT 
GAAAGGAAGA TTGAAGAAAC CCTTGAGGAA ATAAATTTTC TATTATCCTT GGATCTGCAT 
TTCACAGACG GAGATTATTC GGCAGATGAT CTGAACACCT TACAACTAAT AAGTAGTCGA 
ACATTGAAGC TGCACTTTAG CCTCTCTAGA GGACTTCATC ATCATGTTAA TGTTATGTTT 
GATTATTTCC ACCTTTCTGT TGTATCTGTT ACAGTCCATG CATCACTGGT TGCATTACAC 
CAGCCACTTA TAAGCTTTCC TCGCCCTGTG AAGACCACTT GGTTAAACAG AAATGCACCA 
GCACAGAACA AAGATTCTGC AATTCCCACT CTGGAAAGTG TAGTCTTTGG TATTAACTAC 
ACAAAACAAT TATCACCAGA TGGCTGTAGC TTCCTCGTTG CGGAGTCCTT CCTGCATCAT 
GCCTACCGTT TTCATTATAC ACTTTGTGCC ACTTTGCTAC TAGCCTTCAA GGGTTTGCAC 
AACTACTTCA TTACGGTAAC AGAAGAGATT CCCTCTTGTC AGAAACTAGA ACTGGATAAA 
ATGGATGTAG AGGCTCGTCT TGCTGAACTG TGTGAAGAAG TTAAAAAAAT AGAAAATCCT 
GATGAGCTGG CAGAACTGAT AAATATGAAT CTTGCACAAC TTTGCTCACT TCTCATGGCT 
TTATGGGGAC AGTTTCTGGA AGTTATAACA CTACATGAAG AACTAAGAGT GTTACTAGCA 
CAGGAGCACC ATACTTTACG GGTTCGCAGG TTTTCTGAGG CATTCTTTTG TTTTGAACAT 
CCAAGAGAAG CTGCCATTGC ATACCAGGAA CTCCATGCTC AGAGTCATCT ACAGATGTGC 
ACCGCTATCA AAAATACTTC CTTCTGCAGT TCTCTTCCAC CCCTACCTAT TGAATGTAGT 
GAATTAGATG GAGATCTCAA TTCATTACCT ATAATCTTTG AAGATAGGTA TTTAGATTCA 
GTTATTGAAG ACTTAGATGC ACCCTGGACG GGAATTCAGA CTCTTCAGAT ATCAGAGTCC 
AGTAGAATGG ATAAATATGA GACTGAAGAA AGCTCTGTAG CAGGACTTTC TAGCCCAGAG 
TTGAAAGTCA GAACTGCTGG TGCCTCCAGT ATATGCTGTA CAGATGGTGA AAAGCAGCTA 
ACAAAGTCTC AAAAAGGAAA GAATGAAGAA TTAAATAAAT CCAAAGTTAA GGTCACTAAG 
CTCATGAAAA CAATGAAACA AGAGAACACA AAAAAGTTAA TAAAACAGAA CTCTAAAGAT 
TCTGTGATTT TAGTAAGCTA CAGATGTTTG AAAAGTACAC CATCAAGTGA TCTTAGTAAA 
TGCTTTGAAG GCAATCCTGC ACATAGTCAG AAAGAAGGTC TGGATCCCAC AATATGTGGA 
TATAATTTTG ACCCAAAGAC CTACATCAGA CAGACAAGTC AAAAGGAAGC TAGCTCTTTG 
CCAGCTAATA CAGAGAGAAC TGAACAAAAG TCTCCAGATG TTGAAAATGT GCAACCAGAC 
CAGTTTGATC CCTTGAACTC TGGCAGCCTA AATCTTTGTG CGCATTTGTC CATTTCAGGT 
AAACTTGGTA TCTCCCAGGA CGATAGTGAT ATCACACACA TGGAACATAA TCTGGCATCA 
AGAAGTTCAT CAGATGATTT CCATGACTGT CAGACAACCC CATCTTCAGC AGTTAAAACA 
CTTGAAGTAA ATTCCACCAA TAAAAATCCT TTCGATGGAG AGAAAATAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTAG ATAATTTTCT ATCCACTTTT 
GAATCCTTAG ACTCTAATGG TAAATCCAAG TCTATAGAAA TAACACTTGA AAAGGAGGCT 
TTACAGGAAG CAAAATGTCA TTCTTTTGGA GAATCATTAG CTAAATTAAG AAATAATCTA 
CCTGCTCCTA CAAAAGAATA TCATGTTGTA GTAAGTGGAG ATACAATTAA ATTACCAGAT 
ACTAATGCCA CATATGCCTC ATCTAGATTT TCAGATTCAG GTGTTGAAAG TGAACCAAGC 
TCTTTTGCAA CACATCCAAA CCTGGAAATA GTCTCTGAAA CTGGCCAAGG GCAAGGTCCT 
TACCATAATG AAAGGTTATT TCCTCAGCTT TTGATGAAAC CTGATTACAA TGTCAAGTTT 
TCATTAGGAA GTCACTGTAC TGAGAGTACA AGTGCTTTAA GTGAAATACA GTCATCTTTG 
ACATCAATAA ACTCTCTACC TTCTGATGAT GAACTCTCAC CTGATGAAAA TTCTAAGAAA 
TCTTCTGTGC CTGAATGCCA TCTAAGTGAT AGTAAAACTG TATTAAATCT AGGAACAATG 
GATTTGCCAA AGTGTGATGA TACTAAAAAG TCAAGTATTA TTTTGCAGCC TCAATGTGTT 
GTATTTTCAG GGCATTTGGA CAATGAAACT TTAGCAATAC ATTCCTTAAA TTCAAGCACT 
GAAGACCCTT TACAACATGT TTTTTCAGAT AAAGATACTT CTAGTGATGT GAAAAGTAGT 
TGTAGCTCCC AGCCTAATTT GGACCCTATA TTTAAAGGCT CCCAGAGTCA TGATACATCT 
AATAACAACT CTGCAGGAAC CGTGATCGCA ATACAGTCAG AACTGATTTG CTTAGGCACT 
CCTTGTGTTG TTTCGGGTTC TGTTTCTACT AATTCAGAAC TAAGTCAAGA GGGAACTGTG 
GAAAGAAAAA GTAGTGACCC ATTAAGTCTC AAACAAATGT ATTCAGAAGC CCCTTCAATT 
GAACATGAAC TTCATCTGGG TTCGAGTGAC CCTCTTTCAG CCAGTACTGA TATGGTAAAA 
GAAGGGCTTG TGGAAAATTA TTTTGGTTCT CAGAGCAGTA CAGATATTTC GGACATACGT 
GCTGTTAGCT ACCACAACTC AGTTAGCCCT CAGAAGGAAA CCTGTGAAAA AGGAATCAGT 
GACCTTCAGC AGGAACAGGG CAAAGATGAG GAGGAGGAAG AACAGGATCA ACAAATGATT 
CAAAATGGGT ATTATGAAGA AACAGATTTT TCAGCTTTGG GTGGAACTAT AAATGCTCAC 
TTTCCCAGCA GAGATGTGCA AACAGAGGAA AGGCATCTAA AATCTGAAGA AGTGAACAGT 
GACTATCTGA GGGACAGTGT GAATGTGCCT ACTGTTTGTA CTTCGGGTTG TTTGTCTTTC 
CCTTCTGCTC CACGAGAGTC TCCTTGTAGT GTTAAATACT CTTCTAGAAG TAAATTTGAT 
GCCATTACAA AGCAGCCAAG CAGCATTTCT TACAACTTCA CTTCTTCAAT TTCCTGGTAT 
GAAAACTCAC CAAAACCTCA AATACAAGCC TTCCTTCAGG CAAAAGAAGA ACTGAAGCAA 
CTTAAACTCC CTGGGTTCAT GTACAGTGAT GTTCCTCTGC TGGCATCCTC AGTACCTTAT 
TTCAGCATGG AAGAAGAAGA TGGTTCTGAA GATGGTGTCC ATCTCATTGT CTGCGTGCAT 
GGTTTAGATG GTAACAGTGC AGATCTCCGA CTAGTAAAAA CGTATATTGA ACTTGGACTG 
CCTGGGGGAA GAGTTGATTT TCTTATGTCT GAGAGAAATC AGAATGATAC TTTCGCTGAT 
TTTGATAGCA TGACTGATCG TCTTTTGGAT GAAATCATAC AGTATATTCA GATATATAGT 
TTAACAGTCT CAAAAATAAG CTTTATTGGA CATTCATTGG GCAATTTAAT AATCCGTTCA 
GTGCTCACAA GGCCACGGTT TAAATATTAC CTCAGCAAAC TTCATACCTT TCTCTCTCTT 
TCTGGGCCTC ACCTTGGTAC ACTGTACAAT AGCAGTGCTC TCGTCAATAC AGGTCTCTGG 
TTCATGCAGA AATGGAAAAA ATCAGGTTCT CTTTTGCAAC TGACATGTCG AGATCATTCA 
GACCCTCGCC AAACTTTTTT ATATAAGCTT AGTAATAAAG CAGGGCTTCA TTATTTCAAG 
AATGTTGTGC TGGTAGGATC ACTACAGGAT CGCTATGTTC CATATCATTC TGCCCGCATT 
GAAATGTGCA AAACAGCCTT GAAGGACAAA CAGTCAGGAC AAATCTATTC AGAAATGATC 
CACAACTTGC TTCGCCCAGT TCTGCAAAGC AAAGACTGTA ATTTGATTCG ATACAATGTC 
ATCAATGCAT TGCCCAACAC GGCTGATTCA CTCATCGGGA GAGCTGCACA CATAGCTGTT 
CTTGATTCGG AAATATTTTT AGAGAAGTTC TTCCTGGTTG CTGCCCTCAA ATATTTCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Fam135a
Entrez Gene ID	103752489
Full Name	family with sequence similarity 135 member A
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Fam135a cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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