Pik3ap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pik3ap1
Entrez Gene ID	83490
Full Name	phosphoinositide-3-kinase adaptor protein 1
Synonyms	1810044J04Rik,BCAP
General protein information	Preferred Names phosphoinositide-3-kinase adaptor protein 1 Names phosphoinositide 3-kinase adapter protein 1 B cell phosphoinositide 3-kinase adaptor B-cell adapter for phosphoinositide 3-kinase B-cell phosphoinositide 3-kinase adapter protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_031376.3	NP_113553.1	phosphoinositide 3-kinase adapter protein 1
NM_031376.4	NP_113553.1	phosphoinositide 3-kinase adapter protein 1
XM_006527472.3	XP_006527535.1	phosphoinositide 3-kinase adapter protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PIK3AP1	XP_850837.1
Rattus norvegicus (Norway rat)	Pik3ap1	NP_001099838.1
Homo sapiens (human)	PIK3AP1	NP_689522.2
Bos taurus (cattle)	PIK3AP1	XP_002698457.2
Danio rerio (zebrafish)	pik3ap1	XP_005156973.1
Xenopus tropicalis (tropical clawed frog)	pik3ap1	XP_002936021.2
Pan troglodytes (chimpanzee)	PIK3AP1	XP_507955.2
	PIK3AP1	XP_001101530.1
Mus musculus (house mouse)	Pik3ap1	NP_113553.1
Gallus gallus (chicken)	PIK3AP1	NP_989681.1

Gene Ontology
Bibliography

Related articles in PubMed

BCAP promotes osteoclast differentiation through regulation of the p38-dependent CREB signaling pathway.
Kim JH, Kim K, Kim I, Seong S, Lee KB, Kim N
Bone107188-195(2018 Feb)

BCAP inhibits proliferation and differentiation of myeloid progenitors in the steady state and during demand situations.
Duggan JM, Buechler MB, Olson RM, Hohl TM, Hamerman JA
Blood129(11)1503-1513(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identify BCAP as a novel inhibitor of myelopoiesis in the steady state and of emergency myelopoiesis during demand conditions
Title: BCAP inhibits proliferation and differentiation of myeloid progenitors in the steady state and during demand situations.

Nck-mediated recruitment of BCAP to the BCR regulates the PI(3)K-Akt pathway in B cells.
Title: Nck-mediated recruitment of BCAP to the BCR regulates the PI(3)K-Akt pathway in B cells.

BCAP is a unique TIR domain-containing toll like receptor (TLR) signaling adapter crucial for linking TLRs to PI3K activation and regulating the inflammatory response.
Title: Role for B-cell adapter for PI3K (BCAP) as a signaling adapter linking Toll-like receptors (TLRs) to serine/threonine kinases PI3K/Akt.

BCAP is an essential activator of the PI3K pathway downstream of TLR signaling.
Title: B-cell adaptor for PI3K (BCAP) negatively regulates Toll-like receptor signaling through activation of PI3K.

As macrophages engage their pathogenic targets, innate immune receptors trigger increased expression of BCAP, which endows them with the capacity to withstand further challenges from ongoing cellular insults, such as endoplasmic reticulum stress.
Title: A requirement for the p85 PI3K adapter protein BCAP in the protection of macrophages from apoptosis induced by endoplasmic reticulum stress.

The following Pik3ap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pik3ap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55819	XM_006527472.3 Latest version!	Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu08552	NM_031376.4 Latest version!	Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OMu08552	NM_031376.3	Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pik3ap1 ( XM_006527472.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527535.1 Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55819
Clone ID Related Accession (Same CDS sequence)	XM_006527472.3
Accession Version	XM_006527472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphoinositide 3-kinase adapter protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527472.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTAC AGCCGGACCG CATTCGCTGT GGGGCAGAAA CCACTGTGTA TATCATTGTG 
AGATGCAAGC TGGACGAGAA GGTATCAACA GAAGCAGAGT TCTCTCCTGA GGATTCTCCC 
TCCATCCGGG TGGAAGGCAC ACTGGAGAAT GAGTACACGG TCTCTGTGAA AGCACCAGAC 
CTCTCCTCGG GGAATGTTTC TCTGAAGGTC TATTCTGGGG ACCTGGTGGT CTGCGAAACC 
ACCGTCAGCT ATTATACCGA CATGGAGGAA ATTGGGAACT TACTGTCGAG TGCTGCAAAT 
CCTGTGGAGT TTATGTGTCA GGCCTTTAAA ATCGTGCCCT ACAACACAGA GACTCTCGAT 
AAACTGCTCA CCGAGTCCCT GAAGAACAAC ATCCCCGCAA GTGGACTGCA CCTCTTTGGG 
ATCAACCAAC TGGAAGAAGA CGATATGATG ACAAATCAGA GGGACGAAGA GCTGCCCACG 
CTGTTGCATT TTGCTGCCAA GTATGGACTG AAGAACCTCA CTGCCTTGCT GCTCACCTGT 
CCAGGGGCTC TCCAGGCCTA CAGTGTAGCC AACAAGCACG GCCACTATCC CAACACCATT 
GCCGAGAAAC ACGGCTTCAG GGACCTGCGC CAGTTCATCG ACGAGTATGT GGAGACGGTG 
GACATGCTGA AGACACACAT TAAGGAGGAG TTGATGCAAG GGGAGGAGGC GGACGATGTG 
TACGAGTCCA TGGCCCACCT ATCCACAGAC CTGCTGATGA AGTGCTCCCT CAACCCTGGC 
TGTGACGATG AGCTCTACGA GTCCATGGCT GCCTTCGCCC CAGCAGCCAC AGAAGACCTC 
TATGTTGAAA TGCTTCAGGC CAGTGCAGGT AACCCAGTCT CGGGAGAAAG TTTCTCTCGG 
CCCACTAAAG ACTCGATGAT CCGCAAGTTT CTAGAAGGTA ACAGTGTGAA ACCGGCCAGT 
TGGGAGAGAG AACAACACCA TCCCTATGGG GAGGAACTTT ATCACATTGT GGATGAAGAT 
GAGACCTTCT CTGTGGACCT AGCCAACAGG CCCCCTGTCC CTGTGCCCAG GCCAGAGGCC 
AGCGCTCCTG GCCCTCCCCC ACCGCCTGAC AATGAGCCAT ACATTTCTAA AGTTTTTGCA 
GAAAAAAGTC AGGAGCGGCT CGGGAATTTC TACGTTTCTT CTGAGAGCAT CAGAAAAGAG 
CCACTTGTGA GGCCATGGAG GGACAGGCCG CCATCGAGCA TCTACGACCC ATTTGCTGGA 
ATGAAGACCC CGGGCCAGCG GCAGCTCATC ACCCTGCAGG AGCAGGTGAA GCTGGGCATC 
GTCAACGTGG ACGAGGCCGT GCTGCATTTC AAAGAGTGGC AACTCAATCA GAAGAAGCGA 
TCCGAGTCCT TCCGCTTCCA GCAGGAAAAT CTTAAAAGAC TAAGAGAGAG CATCACCCGA 
AGACGGAAAG AGAAGCCAAA GTCTGGGAAG CACACAGACC TGGAGATCAC AGTCCCAATC 
CGACATTCAC AGCACCTGCC AGAGAAAGTG GAGTTTGGCG TGTATGAGAG CGGCCCCAGG 
AAGAGTGTCT TGCCGGCCAG GACAGAGCTG AGACGTGGGG ACTGGAAAAC GGACAGCATG 
TCCAGCACAG CAAGCAGCAC GAGTAACCGG TCGAGCACAC GGAGCCTCCT CAGTGTGAGC 
AGTGGAATGG AGGGCGACAA CGAGGACAAC GAAATCCCCG AAATTACCCG GAGTCGTGGC 
CCAGGTCCTA CGCAGGTGGA CGGAGCTCCA GTTGTGACCG GAACACCAGT CGGGACCCTT 
GAGAGACCCC CTAGGGTGCC TCCTCGAGCC GCTTCTCAGA GGCCTCTAAC CCGGGAGTCC 
TTCCATCCTC CTCCGCCGGT TCCACCCAGA GGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527535.1
CDS	348..2246
Translation	MVVQPDRIRCGAETTVYIIVRCKLDEKVSTEAEFSPEDSPSIRVEGTLENEYTVSVKAPDLSSGNVSLKVYSGDLVVCETTVSYYTDMEEIGNLLSSAANPVEFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEDDMMTNQRDEELPTLLHFAAKYGLKNLTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKTHIKEELMQGEEADDVYESMAHLSTDLLMKCSLNPGCDDELYESMAAFAPAATEDLYVEMLQASAGNPVSGESFSRPTKDSMIRKFLEGNSVKPASWEREQHHPYGEELYHIVDEDETFSVDLANRPPVPVPRPEASAPGPPPPPDNEPYISKVFAEKSQERLGNFYVSSESIRKEPLVRPWRDRPPSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFKEWQLNQKKRSESFRFQQENLKRLRESITRRRKEKPKSGKHTDLEITVPIRHSQHLPEKVEFGVYESGPRKSVLPARTELRRGDWKTDSMSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEIPEITRSRGPGPTQVDGAPVVTGTPVGTLERPPRVPPRAASQRPLTRESFHPPPPVPPRGR

Target ORF information:

RefSeq Version	XM_006527472.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527472.3

ORF Insert Sequence:

ATGGTGGTAC AGCCGGACCG CATTCGCTGT GGGGCAGAAA CCACTGTGTA TATCATTGTG 
AGATGCAAGC TGGACGAGAA GGTATCAACA GAAGCAGAGT TCTCTCCTGA GGATTCTCCC 
TCCATCCGGG TGGAAGGCAC ACTGGAGAAT GAGTACACGG TCTCTGTGAA AGCACCAGAC 
CTCTCCTCGG GGAATGTTTC TCTGAAGGTC TATTCTGGGG ACCTGGTGGT CTGCGAAACC 
ACCGTCAGCT ATTATACCGA CATGGAGGAA ATTGGGAACT TACTGTCGAG TGCTGCAAAT 
CCTGTGGAGT TTATGTGTCA GGCCTTTAAA ATCGTGCCCT ACAACACAGA GACTCTCGAT 
AAACTGCTCA CCGAGTCCCT GAAGAACAAC ATCCCCGCAA GTGGACTGCA CCTCTTTGGG 
ATCAACCAAC TGGAAGAAGA CGATATGATG ACAAATCAGA GGGACGAAGA GCTGCCCACG 
CTGTTGCATT TTGCTGCCAA GTATGGACTG AAGAACCTCA CTGCCTTGCT GCTCACCTGT 
CCAGGGGCTC TCCAGGCCTA CAGTGTAGCC AACAAGCACG GCCACTATCC CAACACCATT 
GCCGAGAAAC ACGGCTTCAG GGACCTGCGC CAGTTCATCG ACGAGTATGT GGAGACGGTG 
GACATGCTGA AGACACACAT TAAGGAGGAG TTGATGCAAG GGGAGGAGGC GGACGATGTG 
TACGAGTCCA TGGCCCACCT ATCCACAGAC CTGCTGATGA AGTGCTCCCT CAACCCTGGC 
TGTGACGATG AGCTCTACGA GTCCATGGCT GCCTTCGCCC CAGCAGCCAC AGAAGACCTC 
TATGTTGAAA TGCTTCAGGC CAGTGCAGGT AACCCAGTCT CGGGAGAAAG TTTCTCTCGG 
CCCACTAAAG ACTCGATGAT CCGCAAGTTT CTAGAAGGTA ACAGTGTGAA ACCGGCCAGT 
TGGGAGAGAG AACAACACCA TCCCTATGGG GAGGAACTTT ATCACATTGT GGATGAAGAT 
GAGACCTTCT CTGTGGACCT AGCCAACAGG CCCCCTGTCC CTGTGCCCAG GCCAGAGGCC 
AGCGCTCCTG GCCCTCCCCC ACCGCCTGAC AATGAGCCAT ACATTTCTAA AGTTTTTGCA 
GAAAAAAGTC AGGAGCGGCT CGGGAATTTC TACGTTTCTT CTGAGAGCAT CAGAAAAGAG 
CCACTTGTGA GGCCATGGAG GGACAGGCCG CCATCGAGCA TCTACGACCC ATTTGCTGGA 
ATGAAGACCC CGGGCCAGCG GCAGCTCATC ACCCTGCAGG AGCAGGTGAA GCTGGGCATC 
GTCAACGTGG ACGAGGCCGT GCTGCATTTC AAAGAGTGGC AACTCAATCA GAAGAAGCGA 
TCCGAGTCCT TCCGCTTCCA GCAGGAAAAT CTTAAAAGAC TAAGAGAGAG CATCACCCGA 
AGACGGAAAG AGAAGCCAAA GTCTGGGAAG CACACAGACC TGGAGATCAC AGTCCCAATC 
CGACATTCAC AGCACCTGCC AGAGAAAGTG GAGTTTGGCG TGTATGAGAG CGGCCCCAGG 
AAGAGTGTCT TGCCGGCCAG GACAGAGCTG AGACGTGGGG ACTGGAAAAC GGACAGCATG 
TCCAGCACAG CAAGCAGCAC GAGTAACCGG TCGAGCACAC GGAGCCTCCT CAGTGTGAGC 
AGTGGAATGG AGGGCGACAA CGAGGACAAC GAAATCCCCG AAATTACCCG GAGTCGTGGC 
CCAGGTCCTA CGCAGGTGGA CGGAGCTCCA GTTGTGACCG GAACACCAGT CGGGACCCTT 
GAGAGACCCC CTAGGGTGCC TCCTCGAGCC GCTTCTCAGA GGCCTCTAAC CCGGGAGTCC 
TTCCATCCTC CTCCGCCGGT TCCACCCAGA GGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pik3ap1 ( NM_031376.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_113553.1 Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), mRNA.

Price & Availability↑

CloneID	OMu08552
Clone ID Related Accession (Same CDS sequence)	NM_031376.3 , NM_031376.4
Accession Version	NM_031376.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Mus musculus(house mouse)
Product	phosphoinositide 3-kinase adapter protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF293806.1. On Jun 10, 2007 this sequence version replaced NM_031376.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF293806.1, AK154326.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCCT CAGGGTGGGG CCGGGGATGC GACATCCTCA TCTTCTACAG CCCAGATGCG 
GAGGAATGGT GTCAGTACCT ACAGGATCTT TTCGTGTCCT GCCGGCAGGT TCGTAGCCAG 
AAGACGCAGA CATACCGGCT GGTCCCGGAT GCCTCTTTCT CTGCCCAGGA CCTGTGGGTC 
TTCAGGGATG CGCGATGCGT GCTGGTGCTA CTGTCGGCGG GGCTGGTAGG ATGCTTCGGC 
CAGCCAGGAC TGCTGCCAAT GCTGCAGCGT GCCTGCCATC CGCCGCAGCG TGTGGTGCGG 
CTGTTGTGTG GAGTGCAGCC AGGCGACGAG GACTTCCAGG CCTTCTTTCC TGACTGGGCC 
CACTGGCAGG AAATGACTTG TGATGATGAG CCAGAGACCT ACCTGGCGGC TGTGAGGAAG 
GCCATTTCTG AAGATTCTGG CTGTGACTCA GTAACCGACA CGGAGCCCGA GGACGAGAGA 
GAGCTTCCTT TCTCAAAGCA GACAAACCTG CCTCCGGAGA TATCGCCTGG GAACCTGATG 
GTGGTACAGC CGGACCGCAT TCGCTGTGGG GCAGAAACCA CTGTGTATAT CATTGTGAGA 
TGCAAGCTGG ACGAGAAGGT ATCAACAGAA GCAGAGTTCT CTCCTGAGGA TTCTCCCTCC 
ATCCGGGTGG AAGGCACACT GGAGAATGAG TACACGGTCT CTGTGAAAGC ACCAGACCTC 
TCCTCGGGGA ATGTTTCTCT GAAGGTCTAT TCTGGGGACC TGGTGGTCTG CGAAACCACC 
GTCAGCTATT ATACCGACAT GGAGGAAATT GGGAACTTAC TGTCGAGTGC TGCAAATCCT 
GTGGAGTTTA TGTGTCAGGC CTTTAAAATC GTGCCCTACA ACACAGAGAC TCTCGATAAA 
CTGCTCACCG AGTCCCTGAA GAACAACATC CCCGCAAGTG GACTGCACCT CTTTGGGATC 
AACCAACTGG AAGAAGACGA TATGATGACA AATCAGAGGG ACGAAGAGCT GCCCACGCTG 
TTGCATTTTG CTGCCAAGTA TGGACTGAAG AACCTCACTG CCTTGCTGCT CACCTGTCCA 
GGGGCTCTCC AGGCCTACAG TGTAGCCAAC AAGCACGGCC ACTATCCCAA CACCATTGCC 
GAGAAACACG GCTTCAGGGA CCTGCGCCAG TTCATCGACG AGTATGTGGA GACGGTGGAC 
ATGCTGAAGA CACACATTAA GGAGGAGTTG ATGCAAGGGG AGGAGGCGGA CGATGTGTAC 
GAGTCCATGG CCCACCTATC CACAGACCTG CTGATGAAGT GCTCCCTCAA CCCTGGCTGT 
GACGATGAGC TCTACGAGTC CATGGCTGCC TTCGCCCCAG CAGCCACAGA AGACCTCTAT 
GTTGAAATGC TTCAGGCCAG TGCAGGTAAC CCAGTCTCGG GAGAAAGTTT CTCTCGGCCC 
ACTAAAGACT CGATGATCCG CAAGTTTCTA GAAGGTAACA GTGTGAAACC GGCCAGTTGG 
GAGAGAGAAC AACACCATCC CTATGGGGAG GAACTTTATC ACATTGTGGA TGAAGATGAG 
ACCTTCTCTG TGGACCTAGC CAACAGGCCC CCTGTCCCTG TGCCCAGGCC AGAGGCCAGC 
GCTCCTGGCC CTCCCCCACC GCCTGACAAT GAGCCATACA TTTCTAAAGT TTTTGCAGAA 
AAAAGTCAGG AGCGGCTCGG GAATTTCTAC GTTTCTTCTG AGAGCATCAG AAAAGAGCCA 
CTTGTGAGGC CATGGAGGGA CAGGCCGCCA TCGAGCATCT ACGACCCATT TGCTGGAATG 
AAGACCCCGG GCCAGCGGCA GCTCATCACC CTGCAGGAGC AGGTGAAGCT GGGCATCGTC 
AACGTGGACG AGGCCGTGCT GCATTTCAAA GAGTGGCAAC TCAATCAGAA GAAGCGATCC 
GAGTCCTTCC GCTTCCAGCA GGAAAATCTT AAAAGACTAA GAGAGAGCAT CACCCGAAGA 
CGGAAAGAGA AGCCAAAGTC TGGGAAGCAC ACAGACCTGG AGATCACAGT CCCAATCCGA 
CATTCACAGC ACCTGCCAGA GAAAGTGGAG TTTGGCGTGT ATGAGAGCGG CCCCAGGAAG 
AGTGTCTTGC CGGCCAGGAC AGAGCTGAGA CGTGGGGACT GGAAAACGGA CAGCATGTCC 
AGCACAGCAA GCAGCACGAG TAACCGGTCG AGCACACGGA GCCTCCTCAG TGTGAGCAGT 
GGAATGGAGG GCGACAACGA GGACAACGAA ATCCCCGAAA TTACCCGGAG TCGTGGCCCA 
GGTCCTACGC AGGTGGACGG AGCTCCAGTT GTGACCGGAA CACCAGTCGG GACCCTTGAG 
AGACCCCCTA GGGTGCCTCC TCGAGCCGCT TCTCAGAGGC CTCTAACCCG GGAGTCCTTC 
CATCCTCCTC CGCCGGTTCC ACCCAGAGGT CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113553.1
CDS	102..2537
Translation	MAASGWGRGCDILIFYSPDAEEWCQYLQDLFVSCRQVRSQKTQTYRLVPDASFSAQDLWVFRDARCVLVLLSAGLVGCFGQPGLLPMLQRACHPPQRVVRLLCGVQPGDEDFQAFFPDWAHWQEMTCDDEPETYLAAVRKAISEDSGCDSVTDTEPEDERELPFSKQTNLPPEISPGNLMVVQPDRIRCGAETTVYIIVRCKLDEKVSTEAEFSPEDSPSIRVEGTLENEYTVSVKAPDLSSGNVSLKVYSGDLVVCETTVSYYTDMEEIGNLLSSAANPVEFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEDDMMTNQRDEELPTLLHFAAKYGLKNLTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKTHIKEELMQGEEADDVYESMAHLSTDLLMKCSLNPGCDDELYESMAAFAPAATEDLYVEMLQASAGNPVSGESFSRPTKDSMIRKFLEGNSVKPASWEREQHHPYGEELYHIVDEDETFSVDLANRPPVPVPRPEASAPGPPPPPDNEPYISKVFAEKSQERLGNFYVSSESIRKEPLVRPWRDRPPSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFKEWQLNQKKRSESFRFQQENLKRLRESITRRRKEKPKSGKHTDLEITVPIRHSQHLPEKVEFGVYESGPRKSVLPARTELRRGDWKTDSMSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEIPEITRSRGPGPTQVDGAPVVTGTPVGTLERPPRVPPRAASQRPLTRESFHPPPPVPPRGR

Target ORF information:

RefSeq Version	NM_031376.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031376.3

ORF Insert Sequence:

ATGGCAGCCT CAGGGTGGGG CCGGGGATGC GACATCCTCA TCTTCTACAG CCCAGATGCG 
GAGGAATGGT GTCAGTACCT ACAGGATCTT TTCGTGTCCT GCCGGCAGGT TCGTAGCCAG 
AAGACGCAGA CATACCGGCT GGTCCCGGAT GCCTCTTTCT CTGCCCAGGA CCTGTGGGTC 
TTCAGGGATG CGCGATGCGT GCTGGTGCTA CTGTCGGCGG GGCTGGTAGG ATGCTTCGGC 
CAGCCAGGAC TGCTGCCAAT GCTGCAGCGT GCCTGCCATC CGCCGCAGCG TGTGGTGCGG 
CTGTTGTGTG GAGTGCAGCC AGGCGACGAG GACTTCCAGG CCTTCTTTCC TGACTGGGCC 
CACTGGCAGG AAATGACTTG TGATGATGAG CCAGAGACCT ACCTGGCGGC TGTGAGGAAG 
GCCATTTCTG AAGATTCTGG CTGTGACTCA GTAACCGACA CGGAGCCCGA GGACGAGAGA 
GAGCTTCCTT TCTCAAAGCA GACAAACCTG CCTCCGGAGA TATCGCCTGG GAACCTGATG 
GTGGTACAGC CGGACCGCAT TCGCTGTGGG GCAGAAACCA CTGTGTATAT CATTGTGAGA 
TGCAAGCTGG ACGAGAAGGT ATCAACAGAA GCAGAGTTCT CTCCTGAGGA TTCTCCCTCC 
ATCCGGGTGG AAGGCACACT GGAGAATGAG TACACGGTCT CTGTGAAAGC ACCAGACCTC 
TCCTCGGGGA ATGTTTCTCT GAAGGTCTAT TCTGGGGACC TGGTGGTCTG CGAAACCACC 
GTCAGCTATT ATACCGACAT GGAGGAAATT GGGAACTTAC TGTCGAGTGC TGCAAATCCT 
GTGGAGTTTA TGTGTCAGGC CTTTAAAATC GTGCCCTACA ACACAGAGAC TCTCGATAAA 
CTGCTCACCG AGTCCCTGAA GAACAACATC CCCGCAAGTG GACTGCACCT CTTTGGGATC 
AACCAACTGG AAGAAGACGA TATGATGACA AATCAGAGGG ACGAAGAGCT GCCCACGCTG 
TTGCATTTTG CTGCCAAGTA TGGACTGAAG AACCTCACTG CCTTGCTGCT CACCTGTCCA 
GGGGCTCTCC AGGCCTACAG TGTAGCCAAC AAGCACGGCC ACTATCCCAA CACCATTGCC 
GAGAAACACG GCTTCAGGGA CCTGCGCCAG TTCATCGACG AGTATGTGGA GACGGTGGAC 
ATGCTGAAGA CACACATTAA GGAGGAGTTG ATGCAAGGGG AGGAGGCGGA CGATGTGTAC 
GAGTCCATGG CCCACCTATC CACAGACCTG CTGATGAAGT GCTCCCTCAA CCCTGGCTGT 
GACGATGAGC TCTACGAGTC CATGGCTGCC TTCGCCCCAG CAGCCACAGA AGACCTCTAT 
GTTGAAATGC TTCAGGCCAG TGCAGGTAAC CCAGTCTCGG GAGAAAGTTT CTCTCGGCCC 
ACTAAAGACT CGATGATCCG CAAGTTTCTA GAAGGTAACA GTGTGAAACC GGCCAGTTGG 
GAGAGAGAAC AACACCATCC CTATGGGGAG GAACTTTATC ACATTGTGGA TGAAGATGAG 
ACCTTCTCTG TGGACCTAGC CAACAGGCCC CCTGTCCCTG TGCCCAGGCC AGAGGCCAGC 
GCTCCTGGCC CTCCCCCACC GCCTGACAAT GAGCCATACA TTTCTAAAGT TTTTGCAGAA 
AAAAGTCAGG AGCGGCTCGG GAATTTCTAC GTTTCTTCTG AGAGCATCAG AAAAGAGCCA 
CTTGTGAGGC CATGGAGGGA CAGGCCGCCA TCGAGCATCT ACGACCCATT TGCTGGAATG 
AAGACCCCGG GCCAGCGGCA GCTCATCACC CTGCAGGAGC AGGTGAAGCT GGGCATCGTC 
AACGTGGACG AGGCCGTGCT GCATTTCAAA GAGTGGCAAC TCAATCAGAA GAAGCGATCC 
GAGTCCTTCC GCTTCCAGCA GGAAAATCTT AAAAGACTAA GAGAGAGCAT CACCCGAAGA 
CGGAAAGAGA AGCCAAAGTC TGGGAAGCAC ACAGACCTGG AGATCACAGT CCCAATCCGA 
CATTCACAGC ACCTGCCAGA GAAAGTGGAG TTTGGCGTGT ATGAGAGCGG CCCCAGGAAG 
AGTGTCTTGC CGGCCAGGAC AGAGCTGAGA CGTGGGGACT GGAAAACGGA CAGCATGTCC 
AGCACAGCAA GCAGCACGAG TAACCGGTCG AGCACACGGA GCCTCCTCAG TGTGAGCAGT 
GGAATGGAGG GCGACAACGA GGACAACGAA ATCCCCGAAA TTACCCGGAG TCGTGGCCCA 
GGTCCTACGC AGGTGGACGG AGCTCCAGTT GTGACCGGAA CACCAGTCGG GACCCTTGAG 
AGACCCCCTA GGGTGCCTCC TCGAGCCGCT TCTCAGAGGC CTCTAACCCG GGAGTCCTTC 
CATCCTCCTC CGCCGGTTCC ACCCAGAGGT CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pik3ap1 ( NM_031376.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_113553.1 Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), mRNA.

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CloneID	OMu08552
Clone ID Related Accession (Same CDS sequence)	NM_031376.3 , NM_031376.4
Accession Version	NM_031376.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	phosphoinositide 3-kinase adapter protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC112271.4 and AC133099.3. On Feb 7, 2019 this sequence version replaced NM_031376.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK171021.1, AK154326.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCCT CAGGGTGGGG CCGGGGATGC GACATCCTCA TCTTCTACAG CCCAGATGCG 
GAGGAATGGT GTCAGTACCT ACAGGATCTT TTCGTGTCCT GCCGGCAGGT TCGTAGCCAG 
AAGACGCAGA CATACCGGCT GGTCCCGGAT GCCTCTTTCT CTGCCCAGGA CCTGTGGGTC 
TTCAGGGATG CGCGATGCGT GCTGGTGCTA CTGTCGGCGG GGCTGGTAGG ATGCTTCGGC 
CAGCCAGGAC TGCTGCCAAT GCTGCAGCGT GCCTGCCATC CGCCGCAGCG TGTGGTGCGG 
CTGTTGTGTG GAGTGCAGCC AGGCGACGAG GACTTCCAGG CCTTCTTTCC TGACTGGGCC 
CACTGGCAGG AAATGACTTG TGATGATGAG CCAGAGACCT ACCTGGCGGC TGTGAGGAAG 
GCCATTTCTG AAGATTCTGG CTGTGACTCA GTAACCGACA CGGAGCCCGA GGACGAGAGA 
GAGCTTCCTT TCTCAAAGCA GACAAACCTG CCTCCGGAGA TATCGCCTGG GAACCTGATG 
GTGGTACAGC CGGACCGCAT TCGCTGTGGG GCAGAAACCA CTGTGTATAT CATTGTGAGA 
TGCAAGCTGG ACGAGAAGGT ATCAACAGAA GCAGAGTTCT CTCCTGAGGA TTCTCCCTCC 
ATCCGGGTGG AAGGCACACT GGAGAATGAG TACACGGTCT CTGTGAAAGC ACCAGACCTC 
TCCTCGGGGA ATGTTTCTCT GAAGGTCTAT TCTGGGGACC TGGTGGTCTG CGAAACCACC 
GTCAGCTATT ATACCGACAT GGAGGAAATT GGGAACTTAC TGTCGAGTGC TGCAAATCCT 
GTGGAGTTTA TGTGTCAGGC CTTTAAAATC GTGCCCTACA ACACAGAGAC TCTCGATAAA 
CTGCTCACCG AGTCCCTGAA GAACAACATC CCCGCAAGTG GACTGCACCT CTTTGGGATC 
AACCAACTGG AAGAAGACGA TATGATGACA AATCAGAGGG ACGAAGAGCT GCCCACGCTG 
TTGCATTTTG CTGCCAAGTA TGGACTGAAG AACCTCACTG CCTTGCTGCT CACCTGTCCA 
GGGGCTCTCC AGGCCTACAG TGTAGCCAAC AAGCACGGCC ACTATCCCAA CACCATTGCC 
GAGAAACACG GCTTCAGGGA CCTGCGCCAG TTCATCGACG AGTATGTGGA GACGGTGGAC 
ATGCTGAAGA CACACATTAA GGAGGAGTTG ATGCAAGGGG AGGAGGCGGA CGATGTGTAC 
GAGTCCATGG CCCACCTATC CACAGACCTG CTGATGAAGT GCTCCCTCAA CCCTGGCTGT 
GACGATGAGC TCTACGAGTC CATGGCTGCC TTCGCCCCAG CAGCCACAGA AGACCTCTAT 
GTTGAAATGC TTCAGGCCAG TGCAGGTAAC CCAGTCTCGG GAGAAAGTTT CTCTCGGCCC 
ACTAAAGACT CGATGATCCG CAAGTTTCTA GAAGGTAACA GTGTGAAACC GGCCAGTTGG 
GAGAGAGAAC AACACCATCC CTATGGGGAG GAACTTTATC ACATTGTGGA TGAAGATGAG 
ACCTTCTCTG TGGACCTAGC CAACAGGCCC CCTGTCCCTG TGCCCAGGCC AGAGGCCAGC 
GCTCCTGGCC CTCCCCCACC GCCTGACAAT GAGCCATACA TTTCTAAAGT TTTTGCAGAA 
AAAAGTCAGG AGCGGCTCGG GAATTTCTAC GTTTCTTCTG AGAGCATCAG AAAAGAGCCA 
CTTGTGAGGC CATGGAGGGA CAGGCCGCCA TCGAGCATCT ACGACCCATT TGCTGGAATG 
AAGACCCCGG GCCAGCGGCA GCTCATCACC CTGCAGGAGC AGGTGAAGCT GGGCATCGTC 
AACGTGGACG AGGCCGTGCT GCATTTCAAA GAGTGGCAAC TCAATCAGAA GAAGCGATCC 
GAGTCCTTCC GCTTCCAGCA GGAAAATCTT AAAAGACTAA GAGAGAGCAT CACCCGAAGA 
CGGAAAGAGA AGCCAAAGTC TGGGAAGCAC ACAGACCTGG AGATCACAGT CCCAATCCGA 
CATTCACAGC ACCTGCCAGA GAAAGTGGAG TTTGGCGTGT ATGAGAGCGG CCCCAGGAAG 
AGTGTCTTGC CGGCCAGGAC AGAGCTGAGA CGTGGGGACT GGAAAACGGA CAGCATGTCC 
AGCACAGCAA GCAGCACGAG TAACCGGTCG AGCACACGGA GCCTCCTCAG TGTGAGCAGT 
GGAATGGAGG GCGACAACGA GGACAACGAA ATCCCCGAAA TTACCCGGAG TCGTGGCCCA 
GGTCCTACGC AGGTGGACGG AGCTCCAGTT GTGACCGGAA CACCAGTCGG GACCCTTGAG 
AGACCCCCTA GGGTGCCTCC TCGAGCCGCT TCTCAGAGGC CTCTAACCCG GGAGTCCTTC 
CATCCTCCTC CGCCGGTTCC ACCCAGAGGT CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113553.1
CDS	134..2569
Translation	MAASGWGRGCDILIFYSPDAEEWCQYLQDLFVSCRQVRSQKTQTYRLVPDASFSAQDLWVFRDARCVLVLLSAGLVGCFGQPGLLPMLQRACHPPQRVVRLLCGVQPGDEDFQAFFPDWAHWQEMTCDDEPETYLAAVRKAISEDSGCDSVTDTEPEDERELPFSKQTNLPPEISPGNLMVVQPDRIRCGAETTVYIIVRCKLDEKVSTEAEFSPEDSPSIRVEGTLENEYTVSVKAPDLSSGNVSLKVYSGDLVVCETTVSYYTDMEEIGNLLSSAANPVEFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEDDMMTNQRDEELPTLLHFAAKYGLKNLTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKTHIKEELMQGEEADDVYESMAHLSTDLLMKCSLNPGCDDELYESMAAFAPAATEDLYVEMLQASAGNPVSGESFSRPTKDSMIRKFLEGNSVKPASWEREQHHPYGEELYHIVDEDETFSVDLANRPPVPVPRPEASAPGPPPPPDNEPYISKVFAEKSQERLGNFYVSSESIRKEPLVRPWRDRPPSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFKEWQLNQKKRSESFRFQQENLKRLRESITRRRKEKPKSGKHTDLEITVPIRHSQHLPEKVEFGVYESGPRKSVLPARTELRRGDWKTDSMSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEIPEITRSRGPGPTQVDGAPVVTGTPVGTLERPPRVPPRAASQRPLTRESFHPPPPVPPRGR

Target ORF information:

RefSeq Version	NM_031376.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide-3-kinase adaptor protein 1 (Pik3ap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031376.4

ORF Insert Sequence:

ATGGCAGCCT CAGGGTGGGG CCGGGGATGC GACATCCTCA TCTTCTACAG CCCAGATGCG 
GAGGAATGGT GTCAGTACCT ACAGGATCTT TTCGTGTCCT GCCGGCAGGT TCGTAGCCAG 
AAGACGCAGA CATACCGGCT GGTCCCGGAT GCCTCTTTCT CTGCCCAGGA CCTGTGGGTC 
TTCAGGGATG CGCGATGCGT GCTGGTGCTA CTGTCGGCGG GGCTGGTAGG ATGCTTCGGC 
CAGCCAGGAC TGCTGCCAAT GCTGCAGCGT GCCTGCCATC CGCCGCAGCG TGTGGTGCGG 
CTGTTGTGTG GAGTGCAGCC AGGCGACGAG GACTTCCAGG CCTTCTTTCC TGACTGGGCC 
CACTGGCAGG AAATGACTTG TGATGATGAG CCAGAGACCT ACCTGGCGGC TGTGAGGAAG 
GCCATTTCTG AAGATTCTGG CTGTGACTCA GTAACCGACA CGGAGCCCGA GGACGAGAGA 
GAGCTTCCTT TCTCAAAGCA GACAAACCTG CCTCCGGAGA TATCGCCTGG GAACCTGATG 
GTGGTACAGC CGGACCGCAT TCGCTGTGGG GCAGAAACCA CTGTGTATAT CATTGTGAGA 
TGCAAGCTGG ACGAGAAGGT ATCAACAGAA GCAGAGTTCT CTCCTGAGGA TTCTCCCTCC 
ATCCGGGTGG AAGGCACACT GGAGAATGAG TACACGGTCT CTGTGAAAGC ACCAGACCTC 
TCCTCGGGGA ATGTTTCTCT GAAGGTCTAT TCTGGGGACC TGGTGGTCTG CGAAACCACC 
GTCAGCTATT ATACCGACAT GGAGGAAATT GGGAACTTAC TGTCGAGTGC TGCAAATCCT 
GTGGAGTTTA TGTGTCAGGC CTTTAAAATC GTGCCCTACA ACACAGAGAC TCTCGATAAA 
CTGCTCACCG AGTCCCTGAA GAACAACATC CCCGCAAGTG GACTGCACCT CTTTGGGATC 
AACCAACTGG AAGAAGACGA TATGATGACA AATCAGAGGG ACGAAGAGCT GCCCACGCTG 
TTGCATTTTG CTGCCAAGTA TGGACTGAAG AACCTCACTG CCTTGCTGCT CACCTGTCCA 
GGGGCTCTCC AGGCCTACAG TGTAGCCAAC AAGCACGGCC ACTATCCCAA CACCATTGCC 
GAGAAACACG GCTTCAGGGA CCTGCGCCAG TTCATCGACG AGTATGTGGA GACGGTGGAC 
ATGCTGAAGA CACACATTAA GGAGGAGTTG ATGCAAGGGG AGGAGGCGGA CGATGTGTAC 
GAGTCCATGG CCCACCTATC CACAGACCTG CTGATGAAGT GCTCCCTCAA CCCTGGCTGT 
GACGATGAGC TCTACGAGTC CATGGCTGCC TTCGCCCCAG CAGCCACAGA AGACCTCTAT 
GTTGAAATGC TTCAGGCCAG TGCAGGTAAC CCAGTCTCGG GAGAAAGTTT CTCTCGGCCC 
ACTAAAGACT CGATGATCCG CAAGTTTCTA GAAGGTAACA GTGTGAAACC GGCCAGTTGG 
GAGAGAGAAC AACACCATCC CTATGGGGAG GAACTTTATC ACATTGTGGA TGAAGATGAG 
ACCTTCTCTG TGGACCTAGC CAACAGGCCC CCTGTCCCTG TGCCCAGGCC AGAGGCCAGC 
GCTCCTGGCC CTCCCCCACC GCCTGACAAT GAGCCATACA TTTCTAAAGT TTTTGCAGAA 
AAAAGTCAGG AGCGGCTCGG GAATTTCTAC GTTTCTTCTG AGAGCATCAG AAAAGAGCCA 
CTTGTGAGGC CATGGAGGGA CAGGCCGCCA TCGAGCATCT ACGACCCATT TGCTGGAATG 
AAGACCCCGG GCCAGCGGCA GCTCATCACC CTGCAGGAGC AGGTGAAGCT GGGCATCGTC 
AACGTGGACG AGGCCGTGCT GCATTTCAAA GAGTGGCAAC TCAATCAGAA GAAGCGATCC 
GAGTCCTTCC GCTTCCAGCA GGAAAATCTT AAAAGACTAA GAGAGAGCAT CACCCGAAGA 
CGGAAAGAGA AGCCAAAGTC TGGGAAGCAC ACAGACCTGG AGATCACAGT CCCAATCCGA 
CATTCACAGC ACCTGCCAGA GAAAGTGGAG TTTGGCGTGT ATGAGAGCGG CCCCAGGAAG 
AGTGTCTTGC CGGCCAGGAC AGAGCTGAGA CGTGGGGACT GGAAAACGGA CAGCATGTCC 
AGCACAGCAA GCAGCACGAG TAACCGGTCG AGCACACGGA GCCTCCTCAG TGTGAGCAGT 
GGAATGGAGG GCGACAACGA GGACAACGAA ATCCCCGAAA TTACCCGGAG TCGTGGCCCA 
GGTCCTACGC AGGTGGACGG AGCTCCAGTT GTGACCGGAA CACCAGTCGG GACCCTTGAG 
AGACCCCCTA GGGTGCCTCC TCGAGCCGCT TCTCAGAGGC CTCTAACCCG GGAGTCCTTC 
CATCCTCCTC CGCCGGTTCC ACCCAGAGGT CGCTGA