Desi2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Desi2
Entrez Gene ID	78825
Full Name	desumoylating isopeptidase 2
Synonyms	5830417C01Rik,DeSI-2,Fam152a,Pppde1
General protein information	Preferred Names desumoylating isopeptidase 2 Names desumoylating isopeptidase 2 PPPDE peptidase domain containing 1 PPPDE peptidase domain-containing protein 1 family with sequence similarity 152, member A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H4

mRNA and Protein(s)

mRNA	Protein	Name
NM_024282.3	NP_077244.1	deubiquitinase DESI2
XM_030243714.1	XP_030099574.1	deubiquitinase DESI2 isoform X1
XM_006497038.3	XP_006497101.1	desumoylating isopeptidase 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DESI2	NP_057160.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012058	XP_320465.2
Drosophila melanogaster (fruit fly)	CG7222	NP_610613.1
Caenorhabditis elegans (roundworm)	F36D4.5	NP_741591.1
Pan troglodytes (chimpanzee)	DESI2	XP_003949780.1
	PPPDE1	XP_001102905.2
Canis lupus familiaris (dog)	DESI2	XP_005622658.1
Bos taurus (cattle)	PPPDE1	NP_001093854.1
Mus musculus (house mouse)	Desi2	NP_077244.1
Rattus norvegicus (Norway rat)	Desi2	NP_001013895.1
Gallus gallus (chicken)	PPPDE1	NP_001008460.1
Danio rerio (zebrafish)	desi2	NP_001003532.1
Arabidopsis thaliana (thale cress)	AT4G17486	NP_567528.2
Arabidopsis thaliana (thale cress)	AT5G47310	NP_199542.1
Oryza sativa (rice)	Os03g0100900	NP_001048648.1
Xenopus tropicalis (tropical clawed frog)	desi2	XP_002942241.1

Gene Ontology
Bibliography

Related articles in PubMed

Comprehensive analysis of expression profile reveals the ubiquitous distribution of PPPDE peptidase domain 1, a Golgi apparatus component, and its implications in clinical cancer.
He Y, Wang J, Gou L, Shen C, Chen L, Yi C, Wei X, Yang J
Biochimie95(7)1466-75(2013 Jul)

DeSUMOylating isopeptidase: a second class of SUMO protease.
Shin EJ, Shin HM, Nam E, Kim WS, Kim JH, Oh BH, Yun Y
EMBO reports13(4)339-46(2012 Apr)

PNAS4 knockout does not induce obviously neurocytes apoptosis and abnormal development in mice brain.
Cai P, Xia X, Li F, Cui Y, Yang Q, Chen M
Molecular biology reports39(1)621-8(2012 Jan)

A novel pro-apoptosis gene PNAS4 that induces apoptosis in A549 human lung adenocarcinoma cells and inhibits tumor growth in mice.
Yan F, Gou L, Yang J, Chen L, Tong A, Tang M, Yuan Z, Yao S, Zhang P, Wei Y
Biochimie91(4)502-7(2009 Apr)

Mit1/Lb9 and Copg2, new members of mouse imprinted genes closely linked to Peg1/Mest(1).
Lee YJ, Park CW, Hahn Y, Park J, Lee J, Yun JH, Hyun B, Chung JH
FEBS letters472(2-3)230-4(2000 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression regulation and biological functions of PPPDE1 in development and carcinogenesis.
Title: Comprehensive analysis of expression profile reveals the ubiquitous distribution of PPPDE peptidase domain 1, a Golgi apparatus component, and its implications in clinical cancer.

PNAS4 was not involved in mice brain development and apoptosis.
Title: PNAS4 knockout does not induce obviously neurocytes apoptosis and abnormal development in mice brain.

overexpression of mouse PNAS4 significantly suppressed Lewis lung carcinoma growth and prolonged survival time through induction of tumor cell apoptosis
Title: A novel pro-apoptosis gene PNAS4 that induces apoptosis in A549 human lung adenocarcinoma cells and inhibits tumor growth in mice.

The following Desi2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Desi2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu28871	XM_006497038.3 Latest version!	Mus musculus desumoylating isopeptidase 2 (Desi2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu08074	NM_024282.3 Latest version!	Mus musculus desumoylating isopeptidase 2 (Desi2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu28871	XM_030243714.1 Latest version!	Mus musculus desumoylating isopeptidase 2 (Desi2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Desi2 ( XM_006497038.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497101.1 Mus musculus desumoylating isopeptidase 2 (Desi2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28871
Clone ID Related Accession (Same CDS sequence)	XM_030243714.1 , XM_006497038.3
Accession Version	XM_006497038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	desumoylating isopeptidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAT ACACCTCATC TATTGGAATT GGAGTTTTTC ATTCTGGAAT TGAAGTATAT 
GGCAGAGAGT TTGCTTATGG TGGCCATCCA TATCCTTTTT CTGGAATATT TGAAATTTCC 
CCAGGAAATG CTTCTGAGCT AGGAGAAACA TTTAAATTTA AAGAAGCTGT TGTTCTAGGA 
AGTACGGACT TTCTAGAAGA TGATATAGAG AAAATTGTAG AAGAACTGGG GAAAGAGTAT 
AAGGGCAACG CCTACCATCT GATGCACAAA AACTGCAATC ACTTTTCTTC AGCTTTATCA 
GAGATTCTCT GTGGGAAAGA GATTCCTCGC TGGATCAACC GGCTGGCCTA CTTCAGCTCC 
TGTATACCCT TTCTACAGAG TTGCCTGCCA AAGGAGTGGC TCACTCCTGC TGCACTTCAG 
TCTAGTGTCA GCCAAGAACT CCAGGATGAA CTAGAAGAAG CAGAGGATGC AGCCGCATCC 
AGTGCCATGG CAAGTGCTGC AGCTGGTGCC AGAACTGGGC GTCACACTAA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497101.1
CDS	3191..3727
Translation	MNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASSAMASAAAGARTGRHTKL

Target ORF information:

RefSeq Version	XM_006497038.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus desumoylating isopeptidase 2 (Desi2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497038.3

ORF Insert Sequence:

ATGAATGAAT ACACCTCATC TATTGGAATT GGAGTTTTTC ATTCTGGAAT TGAAGTATAT 
GGCAGAGAGT TTGCTTATGG TGGCCATCCA TATCCTTTTT CTGGAATATT TGAAATTTCC 
CCAGGAAATG CTTCTGAGCT AGGAGAAACA TTTAAATTTA AAGAAGCTGT TGTTCTAGGA 
AGTACGGACT TTCTAGAAGA TGATATAGAG AAAATTGTAG AAGAACTGGG GAAAGAGTAT 
AAGGGCAACG CCTACCATCT GATGCACAAA AACTGCAATC ACTTTTCTTC AGCTTTATCA 
GAGATTCTCT GTGGGAAAGA GATTCCTCGC TGGATCAACC GGCTGGCCTA CTTCAGCTCC 
TGTATACCCT TTCTACAGAG TTGCCTGCCA AAGGAGTGGC TCACTCCTGC TGCACTTCAG 
TCTAGTGTCA GCCAAGAACT CCAGGATGAA CTAGAAGAAG CAGAGGATGC AGCCGCATCC 
AGTGCCATGG CAAGTGCTGC AGCTGGTGCC AGAACTGGGC GTCACACTAA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Desi2 ( NM_024282.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077244.1 Mus musculus desumoylating isopeptidase 2 (Desi2), mRNA.

Price & Availability↑

CloneID	OMu08074
Clone ID Related Accession (Same CDS sequence)	NM_024282.3
Accession Version	NM_024282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Mus musculus(house mouse)
Product	deubiquitinase DESI2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029574.1, AK049579.1 and AC119928.8. On Dec 19, 2006 this sequence version replaced NM_024282.2. ##Evidence-Data-START## Transcript exon combination :: AK146492.1, SRR1660821.28181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGCTA ACCAGTTAGT GGTGCTCAAC GTCTACGACA TGTACTGGAT GAATGAATAC 
ACCTCATCTA TTGGAATTGG AGTTTTTCAT TCTGGAATTG AAGTATATGG CAGAGAGTTT 
GCTTATGGTG GCCATCCATA TCCTTTTTCT GGAATATTTG AAATTTCCCC AGGAAATGCT 
TCTGAGCTAG GAGAAACATT TAAATTTAAA GAAGCTGTTG TTCTAGGAAG TACGGACTTT 
CTAGAAGATG ATATAGAGAA AATTGTAGAA GAACTGGGGA AAGAGTATAA GGGCAACGCC 
TACCATCTGA TGCACAAAAA CTGCAATCAC TTTTCTTCAG CTTTATCAGA GATTCTCTGT 
GGGAAAGAGA TTCCTCGCTG GATCAACCGG CTGGCCTACT TCAGCTCCTG TATACCCTTT 
CTACAGAGTT GCCTGCCAAA GGAGTGGCTC ACTCCTGCTG CACTTCAGTC TAGTGTCAGC 
CAAGAACTCC AGGATGAACT AGAAGAAGCA GAGGATGCAG CCGCATCCAG TGCCATGGCA 
AGTGCTGCAG CTGGTGCCAG AACTGGGCGT CACACTAAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077244.1
CDS	500..1084
Translation	MGANQLVVLNVYDMYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASSAMASAAAGARTGRHTKL

Target ORF information:

RefSeq Version	NM_024282.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus desumoylating isopeptidase 2 (Desi2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024282.3

ORF Insert Sequence:

ATGGGGGCTA ACCAGTTAGT GGTGCTCAAC GTCTACGACA TGTACTGGAT GAATGAATAC 
ACCTCATCTA TTGGAATTGG AGTTTTTCAT TCTGGAATTG AAGTATATGG CAGAGAGTTT 
GCTTATGGTG GCCATCCATA TCCTTTTTCT GGAATATTTG AAATTTCCCC AGGAAATGCT 
TCTGAGCTAG GAGAAACATT TAAATTTAAA GAAGCTGTTG TTCTAGGAAG TACGGACTTT 
CTAGAAGATG ATATAGAGAA AATTGTAGAA GAACTGGGGA AAGAGTATAA GGGCAACGCC 
TACCATCTGA TGCACAAAAA CTGCAATCAC TTTTCTTCAG CTTTATCAGA GATTCTCTGT 
GGGAAAGAGA TTCCTCGCTG GATCAACCGG CTGGCCTACT TCAGCTCCTG TATACCCTTT 
CTACAGAGTT GCCTGCCAAA GGAGTGGCTC ACTCCTGCTG CACTTCAGTC TAGTGTCAGC 
CAAGAACTCC AGGATGAACT AGAAGAAGCA GAGGATGCAG CCGCATCCAG TGCCATGGCA 
AGTGCTGCAG CTGGTGCCAG AACTGGGCGT CACACTAAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Desi2 ( XM_030243714.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099574.1 Mus musculus desumoylating isopeptidase 2 (Desi2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28871
Clone ID Related Accession (Same CDS sequence)	XM_030243714.1 , XM_006497038.3
Accession Version	XM_030243714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deubiquitinase DESI2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAT ACACCTCATC TATTGGAATT GGAGTTTTTC ATTCTGGAAT TGAAGTATAT 
GGCAGAGAGT TTGCTTATGG TGGCCATCCA TATCCTTTTT CTGGAATATT TGAAATTTCC 
CCAGGAAATG CTTCTGAGCT AGGAGAAACA TTTAAATTTA AAGAAGCTGT TGTTCTAGGA 
AGTACGGACT TTCTAGAAGA TGATATAGAG AAAATTGTAG AAGAACTGGG GAAAGAGTAT 
AAGGGCAACG CCTACCATCT GATGCACAAA AACTGCAATC ACTTTTCTTC AGCTTTATCA 
GAGATTCTCT GTGGGAAAGA GATTCCTCGC TGGATCAACC GGCTGGCCTA CTTCAGCTCC 
TGTATACCCT TTCTACAGAG TTGCCTGCCA AAGGAGTGGC TCACTCCTGC TGCACTTCAG 
TCTAGTGTCA GCCAAGAACT CCAGGATGAA CTAGAAGAAG CAGAGGATGC AGCCGCATCC 
AGTGCCATGG CAAGTGCTGC AGCTGGTGCC AGAACTGGGC GTCACACTAA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099574.1
CDS	1006..1542
Translation	MNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAASSAMASAAAGARTGRHTKL

Target ORF information:

RefSeq Version	XM_030243714.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus desumoylating isopeptidase 2 (Desi2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243714.1

ORF Insert Sequence:

ATGAATGAAT ACACCTCATC TATTGGAATT GGAGTTTTTC ATTCTGGAAT TGAAGTATAT 
GGCAGAGAGT TTGCTTATGG TGGCCATCCA TATCCTTTTT CTGGAATATT TGAAATTTCC 
CCAGGAAATG CTTCTGAGCT AGGAGAAACA TTTAAATTTA AAGAAGCTGT TGTTCTAGGA 
AGTACGGACT TTCTAGAAGA TGATATAGAG AAAATTGTAG AAGAACTGGG GAAAGAGTAT 
AAGGGCAACG CCTACCATCT GATGCACAAA AACTGCAATC ACTTTTCTTC AGCTTTATCA 
GAGATTCTCT GTGGGAAAGA GATTCCTCGC TGGATCAACC GGCTGGCCTA CTTCAGCTCC 
TGTATACCCT TTCTACAGAG TTGCCTGCCA AAGGAGTGGC TCACTCCTGC TGCACTTCAG 
TCTAGTGTCA GCCAAGAACT CCAGGATGAA CTAGAAGAAG CAGAGGATGC AGCCGCATCC 
AGTGCCATGG CAAGTGCTGC AGCTGGTGCC AGAACTGGGC GTCACACTAA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comprehensive analysis of expression profile reveals the ubiquitous distribution of PPPDE peptidase domain 1, a Golgi apparatus component, and its implications in clinical cancer.
Biochimie95(7)1466-75(2013 Jul)
He Y,Wang J,Gou L,Shen C,Chen L,Yi C,Wei X,Yang J

DeSUMOylating isopeptidase: a second class of SUMO protease.
EMBO reports13(4)339-46(2012 Apr)
Shin EJ,Shin HM,Nam E,Kim WS,Kim JH,Oh BH,Yun Y

PNAS4 knockout does not induce obviously neurocytes apoptosis and abnormal development in mice brain.
Molecular biology reports39(1)621-8(2012 Jan)
Cai P,Xia X,Li F,Cui Y,Yang Q,Chen M

A novel pro-apoptosis gene PNAS4 that induces apoptosis in A549 human lung adenocarcinoma cells and inhibits tumor growth in mice.
Biochimie91(4)502-7(2009 Apr)
Yan F,Gou L,Yang J,Chen L,Tong A,Tang M,Yuan Z,Yao S,Zhang P,Wei Y

Mit1/Lb9 and Copg2, new members of mouse imprinted genes closely linked to Peg1/Mest(1).
FEBS letters472(2-3)230-4(2000 Apr)
Lee YJ,Park CW,Hahn Y,Park J,Lee J,Yun JH,Hyun B,Chung JH