Usp54 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006519703.3	XP_006519766.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_030248096.1	XP_030103956.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
NM_030180.2	NP_084456.2	inactive ubiquitin carboxyl-terminal hydrolase 54
XM_011245225.2	XP_011243527.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_011245227.2	XP_011243529.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
XM_006519705.4	XP_006519768.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
XM_006519705.3	XP_006519768.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
XM_011245225.3	XP_011243527.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_011245226.3	XP_011243528.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_017316224.1	XP_017171713.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
XM_030248095.1	XP_030103955.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_011245227.3	XP_011243529.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
XM_011245226.2	XP_011243528.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_006519703.4	XP_006519766.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50457	XM_017316224.1 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71758	XM_011245227.3 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu86529	XM_030248096.1 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu08033	XM_006519705.4 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu50452	XM_006519703.4 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu50452	XM_011245225.3 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu50452	XM_030248095.1 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu71757	XM_011245226.3 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu08033	NM_030180.2 Latest version!	Mus musculus ubiquitin specific peptidase 54 (Usp54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu08033	XM_006519705.3	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu50452	XM_011245225.2	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu71758	XM_011245227.2	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu71757	XM_011245226.2	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu50452	XM_006519703.3	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

Usp54 ( XM_017316224.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171713.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu50457
Clone ID Related Accession (Same CDS sequence)	XM_017316224.1
Accession Version	XM_017316224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGT GAACAGCCTA TCACGCTCCA AGTATTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171713.1
CDS	6161..8551
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQVNSLSRSKYC

Target ORF information:

RefSeq Version	XM_017316224.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316224.1

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGT GAACAGCCTA TCACGCTCCA AGTATTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_011245227.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243529.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu71758
Clone ID Related Accession (Same CDS sequence)	XM_011245227.2 , XM_011245227.3
Accession Version	XM_011245227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGAACTTCAA 
GGAGTGTCCA CCAGTTTCTG GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT 
ACACAGGAAG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243529.1
CDS	4253..8530
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQELQGVSTSFWLCVTGMKLPKGSSTQEGL

Target ORF information:

RefSeq Version	XM_011245227.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245227.3

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGAACTTCAA 
GGAGTGTCCA CCAGTTTCTG GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT 
ACACAGGAAG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_030248096.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103956.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X7, mRNA.

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CloneID	OMu86529
Clone ID Related Accession (Same CDS sequence)	XM_030248096.1
Accession Version	XM_030248096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCACA GCAGGCCTTC CCTGGCTTCT CAGACCAGTG CAGGATCTCC CTGTGTAGGT 
AGGGCAGGAG ACCAGCTAGA CAAGATACCA CCAAGGAACT TCCCTTTACA GGCTCGGGGC 
TGGGAAACGG AGAGTACCAG TAGTGAGGCA AAATCCAGTT CTTCCAGCAA GTACCGCCCA 
ACATGGAGAC CTAAACGAGA ATCTCTAAAT ATTGACAGCA TTTTTAGTAA GGACAGAAGG 
AAGCATTGTG GCTACACCCA GCTTAGGACC TTTCCTGAAG ATGCAGCTAA AGAATTTACA 
CCGGATGAAG TAAGCAAGCC AACTGCTAAT GACATTAAAG ATGGCGGATC AAGGTCACAG 
CATAAACTCT GGGGAACAGC TCGGCCAGGC TCTCATCTTT TAGAGCAGCA TCCTCGCCTA 
ATCCAGCGGA TGGAATCTGG CTATGAGAGC AGTGAGAGGA ACAGCAGCAG CCCTGTCAGT 
TTGGATGCTG CCCCGCCTGA CAGCGTGAAT GTCTACAGGG ATCAAAGCAC AAAGAGGCCT 
GTTGGGTTTG TCCCTTCTTG GCGTCATATT CCAAAGTCTC ACAGCAGTAG CATCCTGGAG 
GTAGACTGTA CAGCACCCAT GACCAGCTGG ACAAAGACTC AACCTCTCTC AGATGGGGAG 
GTCACCTCTA GAAGTGAATT GGATGAACTA CAGGAAGAAG TGGTCAGGCG AGCCCAGGAA 
CAGGAGCTGC GGAAGAAACG GGAGAAGGAA TTAGAGGCTG CTAAAGGCTT TAACCCTCAT 
CCCAGCCGCT ACATGGATTT GGATGAACTG CAGAATCAGG GGAGGAGTGA CGGCTTTGAA 
AGGTCCTTGC AAGAGGCAAA TTCAATATTT GAAGAGTCGC TGCATCTGGA ACAAAAGGGA 
GACTGTGCTG CAGCTCTGGC TCTGTGTAAC GAAGCTATCT CTAAACTAAG ACTTACCCTG 
CATGATGCCA GCTCTAGCAC GCACAGCAGA GCCCTAGTCG ATAAGAAGTT GCAAATCAGT 
ATTCGAAAAG CACGAAGCCT TCAGGATCGC ATGCAGCAAC AAGCATCATC GCAGCAGCCG 
GTGCAGCCCT CAGCCTCTCT CCCATCACAG GGGGGCGCCC TCCCTCAGCC GACAAGTGAA 
CAGCCTATCA CGCTCCAAGT ATTGTTAAAC CAGGAGGCAC AGCTGGAACC TTGCAAGGAT 
ACAGAGCTTG GGGCCACTTC CTCTTTCTTC CACTCACCTG CTTCCTGCCC TGAGTTACAC 
TCATCACTCA TCCCAGAGTC ACCTGTTTCA TCTGTTTCAC AGCACAGTCC CCCTGGGACA 
TCTTCCTTGA AGCTTCTGAC GTCTTTTGAG GTGGACAGCG TCAATCGCTC TGCATTCCAC 
AGGCAGGGTT TACCTAAAGC AACAGGTAGA ACTGAGATGA ACTCTCAACA TGAATGCCTG 
CCATTAGATG CCCTTGAAGA TAGGCTCCAA GGCCACAGGG AGGACAACAG CTGTTGCAGC 
AAATTCCCAC CACGAGAAGG AAGAGACATA GCTCAAGATC AGCTGTTTGA AGGAAAAAAG 
AATCCTGCTG ATATATCCAT GGGGATGCCT TGGTCATATT CAACAGGAGA AGCCACTTCT 
GAGAGAGTTA TATATAGCCT AAATAGTCCT TCAAGTTCAT CAGCTCAGCC TAGTATTCCC 
CCTTACAGTT CCTGCCACCC CATCACGTCT GCTGCTTCTT CACCTGTGCT TCATGCTGCT 
GACCCCATGC AGAAACTTAA CCAACACGTT CAAGCCCAAA GTCTCCAGAC TTCATTGACT 
TCAAAAGTGG TCAGAAGCAG TGAGGAGCCC TATAGGCTAG AGTTCCCCAG TACAAAAGGA 
CTTGTCCGCT CATTGGCAGA GCAATTCCAG AAGATGCAGA ATACTTCCAC GAGAGATGTT 
ATAGGTTCCC AGGATAGAAG TTTGCCAAAT GGTGTCAGGA AGAGCTCTTC TCCTTCTGAC 
TTTATGCCTC CATTATCCCA GGGTCCAGGA AGAGAGCACT GTCGCTGGGT AAAACAACCC 
CGTTCCCCCG ATGGCAGGGA AAGACCGCCC TGCTGGGAAG ACCCTGCAGC TCATCCTCCT 
CTTTCCATGG GCTCGGGGCT TCCTGATGGT GAAACCTCGA GGACAGCACA GCCCAGGCTG 
GCTGAGCCAG ATATGTACCA AGGAAAGCTA CCCCAAGTGA CAGATATTAG GTCCAAGGAG 
CTGGGTAGTA GTGTCAATTT GGGGACTTCT TTGCCTTTGG ATTCTTGGGT AAATGTCACA 
AGGCTCTGTG ATTCTCAGGT TAAACCCAGT GCCCCTGGGC CCGGAGACAA GTCCTCCTCC 
CATGATTCCC ACCCACGTGC AACCTGCCTA GAAAGAAGTC CTATCCTTTT GCATCCACAC 
TGGGACCAAG ACACAGAGCA GGAGACCTCT GAACTGGAGT CTCTCTACCA AGCCAGCCTT 
CAGGCCTCTT CTCACACTGC CTATTCTGAT TGGAGATCAC AGGATGTGGC CTGGCAGCCA 
CTTAGCCTAG CAGGCTCTGC AGATGGCATG GGAAGGAGAC TGCACTCAGC CCCTGGGCTT 
GATCTCTCAA AGCCTCTGAC AGCAGAGATG GAGCATGTCC TTTATGAACC GAGCACAGTG 
CCTGTCTCTC AGGATTCATC AAACGTGAGG AAAAAGACTT TGGAAACCGG GCACCATTGT 
TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GATCCTCCTG TATTTCTCCT TGATCCTAAA 
CTCTACCCTC CCCAACCACA GTTCCTGTCC CCAGATGTCC TGATGCCCAG CATGGCAGGG 
GAGCCCTATA GACCCCCAGG AACTTCAAGG AGTGTCCACC AGTTTCTGGC TATGTGTGAC 
AGGGATGAAA CTCCCCAAGG GGTCAAGTAC ACAGGAAGGA CTTTGAACTA CCGAAGTCTA 
CCTCATCGTT CCAGAACTGA TGCTTCCTGG GGACCAGGGT CAGAGACCAA CCAGCACATT 
GGGGCCAGAG TCCTAACTAT GCCAGCATGC AAGCCTCAGC TAACCTACAC TGCCACACTA 
CCAGAAAGAC ACCAGGGCCT TCAGGTTCCA CATGCTCAGT CCTGGGGGAA TCTTTTCCAC 
TCACCCTCCC ATCCTTCTGC TGTTCACCCA GGGTCCCCAC CATCCAGCAA TCTTCATGTA 
CCCCTTAGGT CAACTTGGAA TTCTGGTCCT GTTCTGGGGT CCCGAACACC TGGTCCACGA 
AGAATAGACA TGCCCCCAGA TGATGACTGG AGACAGAGCA GTTACCCCTC CCAGGACAGA 
CACAGGAGCC CAGGAGAGGA AAGAATTATG TTTGCTCTAT CAAATGCTGC TGGAAGAGAG 
CAGAACAGGG TGAGATTTCT ACAGCACAGC AGGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103956.1
CDS	5850..9248
Translation	MIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_030248096.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248096.1

ORF Insert Sequence:

ATGATCCACA GCAGGCCTTC CCTGGCTTCT CAGACCAGTG CAGGATCTCC CTGTGTAGGT 
AGGGCAGGAG ACCAGCTAGA CAAGATACCA CCAAGGAACT TCCCTTTACA GGCTCGGGGC 
TGGGAAACGG AGAGTACCAG TAGTGAGGCA AAATCCAGTT CTTCCAGCAA GTACCGCCCA 
ACATGGAGAC CTAAACGAGA ATCTCTAAAT ATTGACAGCA TTTTTAGTAA GGACAGAAGG 
AAGCATTGTG GCTACACCCA GCTTAGGACC TTTCCTGAAG ATGCAGCTAA AGAATTTACA 
CCGGATGAAG TAAGCAAGCC AACTGCTAAT GACATTAAAG ATGGCGGATC AAGGTCACAG 
CATAAACTCT GGGGAACAGC TCGGCCAGGC TCTCATCTTT TAGAGCAGCA TCCTCGCCTA 
ATCCAGCGGA TGGAATCTGG CTATGAGAGC AGTGAGAGGA ACAGCAGCAG CCCTGTCAGT 
TTGGATGCTG CCCCGCCTGA CAGCGTGAAT GTCTACAGGG ATCAAAGCAC AAAGAGGCCT 
GTTGGGTTTG TCCCTTCTTG GCGTCATATT CCAAAGTCTC ACAGCAGTAG CATCCTGGAG 
GTAGACTGTA CAGCACCCAT GACCAGCTGG ACAAAGACTC AACCTCTCTC AGATGGGGAG 
GTCACCTCTA GAAGTGAATT GGATGAACTA CAGGAAGAAG TGGTCAGGCG AGCCCAGGAA 
CAGGAGCTGC GGAAGAAACG GGAGAAGGAA TTAGAGGCTG CTAAAGGCTT TAACCCTCAT 
CCCAGCCGCT ACATGGATTT GGATGAACTG CAGAATCAGG GGAGGAGTGA CGGCTTTGAA 
AGGTCCTTGC AAGAGGCAAA TTCAATATTT GAAGAGTCGC TGCATCTGGA ACAAAAGGGA 
GACTGTGCTG CAGCTCTGGC TCTGTGTAAC GAAGCTATCT CTAAACTAAG ACTTACCCTG 
CATGATGCCA GCTCTAGCAC GCACAGCAGA GCCCTAGTCG ATAAGAAGTT GCAAATCAGT 
ATTCGAAAAG CACGAAGCCT TCAGGATCGC ATGCAGCAAC AAGCATCATC GCAGCAGCCG 
GTGCAGCCCT CAGCCTCTCT CCCATCACAG GGGGGCGCCC TCCCTCAGCC GACAAGTGAA 
CAGCCTATCA CGCTCCAAGT ATTGTTAAAC CAGGAGGCAC AGCTGGAACC TTGCAAGGAT 
ACAGAGCTTG GGGCCACTTC CTCTTTCTTC CACTCACCTG CTTCCTGCCC TGAGTTACAC 
TCATCACTCA TCCCAGAGTC ACCTGTTTCA TCTGTTTCAC AGCACAGTCC CCCTGGGACA 
TCTTCCTTGA AGCTTCTGAC GTCTTTTGAG GTGGACAGCG TCAATCGCTC TGCATTCCAC 
AGGCAGGGTT TACCTAAAGC AACAGGTAGA ACTGAGATGA ACTCTCAACA TGAATGCCTG 
CCATTAGATG CCCTTGAAGA TAGGCTCCAA GGCCACAGGG AGGACAACAG CTGTTGCAGC 
AAATTCCCAC CACGAGAAGG AAGAGACATA GCTCAAGATC AGCTGTTTGA AGGAAAAAAG 
AATCCTGCTG ATATATCCAT GGGGATGCCT TGGTCATATT CAACAGGAGA AGCCACTTCT 
GAGAGAGTTA TATATAGCCT AAATAGTCCT TCAAGTTCAT CAGCTCAGCC TAGTATTCCC 
CCTTACAGTT CCTGCCACCC CATCACGTCT GCTGCTTCTT CACCTGTGCT TCATGCTGCT 
GACCCCATGC AGAAACTTAA CCAACACGTT CAAGCCCAAA GTCTCCAGAC TTCATTGACT 
TCAAAAGTGG TCAGAAGCAG TGAGGAGCCC TATAGGCTAG AGTTCCCCAG TACAAAAGGA 
CTTGTCCGCT CATTGGCAGA GCAATTCCAG AAGATGCAGA ATACTTCCAC GAGAGATGTT 
ATAGGTTCCC AGGATAGAAG TTTGCCAAAT GGTGTCAGGA AGAGCTCTTC TCCTTCTGAC 
TTTATGCCTC CATTATCCCA GGGTCCAGGA AGAGAGCACT GTCGCTGGGT AAAACAACCC 
CGTTCCCCCG ATGGCAGGGA AAGACCGCCC TGCTGGGAAG ACCCTGCAGC TCATCCTCCT 
CTTTCCATGG GCTCGGGGCT TCCTGATGGT GAAACCTCGA GGACAGCACA GCCCAGGCTG 
GCTGAGCCAG ATATGTACCA AGGAAAGCTA CCCCAAGTGA CAGATATTAG GTCCAAGGAG 
CTGGGTAGTA GTGTCAATTT GGGGACTTCT TTGCCTTTGG ATTCTTGGGT AAATGTCACA 
AGGCTCTGTG ATTCTCAGGT TAAACCCAGT GCCCCTGGGC CCGGAGACAA GTCCTCCTCC 
CATGATTCCC ACCCACGTGC AACCTGCCTA GAAAGAAGTC CTATCCTTTT GCATCCACAC 
TGGGACCAAG ACACAGAGCA GGAGACCTCT GAACTGGAGT CTCTCTACCA AGCCAGCCTT 
CAGGCCTCTT CTCACACTGC CTATTCTGAT TGGAGATCAC AGGATGTGGC CTGGCAGCCA 
CTTAGCCTAG CAGGCTCTGC AGATGGCATG GGAAGGAGAC TGCACTCAGC CCCTGGGCTT 
GATCTCTCAA AGCCTCTGAC AGCAGAGATG GAGCATGTCC TTTATGAACC GAGCACAGTG 
CCTGTCTCTC AGGATTCATC AAACGTGAGG AAAAAGACTT TGGAAACCGG GCACCATTGT 
TCCAGCTCCT CTTCCCTCCC TGTCATCCAT GATCCTCCTG TATTTCTCCT TGATCCTAAA 
CTCTACCCTC CCCAACCACA GTTCCTGTCC CCAGATGTCC TGATGCCCAG CATGGCAGGG 
GAGCCCTATA GACCCCCAGG AACTTCAAGG AGTGTCCACC AGTTTCTGGC TATGTGTGAC 
AGGGATGAAA CTCCCCAAGG GGTCAAGTAC ACAGGAAGGA CTTTGAACTA CCGAAGTCTA 
CCTCATCGTT CCAGAACTGA TGCTTCCTGG GGACCAGGGT CAGAGACCAA CCAGCACATT 
GGGGCCAGAG TCCTAACTAT GCCAGCATGC AAGCCTCAGC TAACCTACAC TGCCACACTA 
CCAGAAAGAC ACCAGGGCCT TCAGGTTCCA CATGCTCAGT CCTGGGGGAA TCTTTTCCAC 
TCACCCTCCC ATCCTTCTGC TGTTCACCCA GGGTCCCCAC CATCCAGCAA TCTTCATGTA 
CCCCTTAGGT CAACTTGGAA TTCTGGTCCT GTTCTGGGGT CCCGAACACC TGGTCCACGA 
AGAATAGACA TGCCCCCAGA TGATGACTGG AGACAGAGCA GTTACCCCTC CCAGGACAGA 
CACAGGAGCC CAGGAGAGGA AAGAATTATG TTTGCTCTAT CAAATGCTGC TGGAAGAGAG 
CAGAACAGGG TGAGATTTCT ACAGCACAGC AGGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_006519705.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519768.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X4, mRNA.

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CloneID	OMu08033
Clone ID Related Accession (Same CDS sequence)	NM_030180.2 , XM_006519705.4 , XM_006519705.3
Accession Version	XM_006519705.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519705.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCGAAGAA AGGGTCCCTG 
GTGGAGCGCA GGAGGAGCAG TGGCCGTGTT CGGAGAAAAG GCGATGAGCC ACAGGCCTCA 
GGGTACCACA GCGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCTAAA 
TCCAGCAGCA GCAGCAGGCT AAAGGATTTC AAAGAGACAG TCAGCAACAT GATCCACAGC 
AGGCCTTCCC TGGCTTCTCA GACCAGTGCA GGATCTCCCT GTGTAGGTAG GGCAGGAGAC 
CAGCTAGACA AGATACCACC AAGGAACTTC CCTTTACAGG CTCGGGGCTG GGAAACGGAG 
AGTACCAGTA GTGAGGCAAA ATCCAGTTCT TCCAGCAAGT ACCGCCCAAC ATGGAGACCT 
AAACGAGAAT CTCTAAATAT TGACAGCATT TTTAGTAAGG ACAGAAGGAA GCATTGTGGC 
TACACCCAGC TTAGGACCTT TCCTGAAGAT GCAGCTAAAG AATTTACACC GGATGAAGTA 
AGCAAGCCAA CTGCTAATGA CATTAAAGAT GGCGGATCAA GGTCACAGCA TAAACTCTGG 
GGAACAGCTC GGCCAGGCTC TCATCTTTTA GAGCAGCATC CTCGCCTAAT CCAGCGGATG 
GAATCTGGCT ATGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGTTT GGATGCTGCC 
CCGCCTGACA GCGTGAATGT CTACAGGGAT CAAAGCACAA AGAGGCCTGT TGGGTTTGTC 
CCTTCTTGGC GTCATATTCC AAAGTCTCAC AGCAGTAGCA TCCTGGAGGT AGACTGTACA 
GCACCCATGA CCAGCTGGAC AAAGACTCAA CCTCTCTCAG ATGGGGAGGT CACCTCTAGA 
AGTGAATTGG ATGAACTACA GGAAGAAGTG GTCAGGCGAG CCCAGGAACA GGAGCTGCGG 
AAGAAACGGG AGAAGGAATT AGAGGCTGCT AAAGGCTTTA ACCCTCATCC CAGCCGCTAC 
ATGGATTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAAAG GTCCTTGCAA 
GAGGCAAATT CAATATTTGA AGAGTCGCTG CATCTGGAAC AAAAGGGAGA CTGTGCTGCA 
GCTCTGGCTC TGTGTAACGA AGCTATCTCT AAACTAAGAC TTACCCTGCA TGATGCCAGC 
TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGAAGCCTTC AGGATCGCAT GCAGCAACAA GCATCATCGC AGCAGCCGGT GCAGCCCTCA 
GCCTCTCTCC CATCACAGGG GGGCGCCCTC CCTCAGCCGA CAAGTGAACA GCCTATCACG 
CTCCAAGTAT TGTTAAACCA GGAGGCACAG CTGGAACCTT GCAAGGATAC AGAGCTTGGG 
GCCACTTCCT CTTTCTTCCA CTCACCTGCT TCCTGCCCTG AGTTACACTC ATCACTCATC 
CCAGAGTCAC CTGTTTCATC TGTTTCACAG CACAGTCCCC CTGGGACATC TTCCTTGAAG 
CTTCTGACGT CTTTTGAGGT GGACAGCGTC AATCGCTCTG CATTCCACAG GCAGGGTTTA 
CCTAAAGCAA CAGGTAGAAC TGAGATGAAC TCTCAACATG AATGCCTGCC ATTAGATGCC 
CTTGAAGATA GGCTCCAAGG CCACAGGGAG GACAACAGCT GTTGCAGCAA ATTCCCACCA 
CGAGAAGGAA GAGACATAGC TCAAGATCAG CTGTTTGAAG GAAAAAAGAA TCCTGCTGAT 
ATATCCATGG GGATGCCTTG GTCATATTCA ACAGGAGAAG CCACTTCTGA GAGAGTTATA 
TATAGCCTAA ATAGTCCTTC AAGTTCATCA GCTCAGCCTA GTATTCCCCC TTACAGTTCC 
TGCCACCCCA TCACGTCTGC TGCTTCTTCA CCTGTGCTTC ATGCTGCTGA CCCCATGCAG 
AAACTTAACC AACACGTTCA AGCCCAAAGT CTCCAGACTT CATTGACTTC AAAAGTGGTC 
AGAAGCAGTG AGGAGCCCTA TAGGCTAGAG TTCCCCAGTA CAAAAGGACT TGTCCGCTCA 
TTGGCAGAGC AATTCCAGAA GATGCAGAAT ACTTCCACGA GAGATGTTAT AGGTTCCCAG 
GATAGAAGTT TGCCAAATGG TGTCAGGAAG AGCTCTTCTC CTTCTGACTT TATGCCTCCA 
TTATCCCAGG GTCCAGGAAG AGAGCACTGT CGCTGGGTAA AACAACCCCG TTCCCCCGAT 
GGCAGGGAAA GACCGCCCTG CTGGGAAGAC CCTGCAGCTC ATCCTCCTCT TTCCATGGGC 
TCGGGGCTTC CTGATGGTGA AACCTCGAGG ACAGCACAGC CCAGGCTGGC TGAGCCAGAT 
ATGTACCAAG GAAAGCTACC CCAAGTGACA GATATTAGGT CCAAGGAGCT GGGTAGTAGT 
GTCAATTTGG GGACTTCTTT GCCTTTGGAT TCTTGGGTAA ATGTCACAAG GCTCTGTGAT 
TCTCAGGTTA AACCCAGTGC CCCTGGGCCC GGAGACAAGT CCTCCTCCCA TGATTCCCAC 
CCACGTGCAA CCTGCCTAGA AAGAAGTCCT ATCCTTTTGC ATCCACACTG GGACCAAGAC 
ACAGAGCAGG AGACCTCTGA ACTGGAGTCT CTCTACCAAG CCAGCCTTCA GGCCTCTTCT 
CACACTGCCT ATTCTGATTG GAGATCACAG GATGTGGCCT GGCAGCCACT TAGCCTAGCA 
GGCTCTGCAG ATGGCATGGG AAGGAGACTG CACTCAGCCC CTGGGCTTGA TCTCTCAAAG 
CCTCTGACAG CAGAGATGGA GCATGTCCTT TATGAACCGA GCACAGTGCC TGTCTCTCAG 
GATTCATCAA ACGTGAGGAA AAAGACTTTG GAAACCGGGC ACCATTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA TCCTCCTGTA TTTCTCCTTG ATCCTAAACT CTACCCTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCAGCA TGGCAGGGGA GCCCTATAGA 
CCCCCAGGAA CTTCAAGGAG TGTCCACCAG TTTCTGGCTA TGTGTGACAG GGATGAAACT 
CCCCAAGGGG TCAAGTACAC AGGAAGGACT TTGAACTACC GAAGTCTACC TCATCGTTCC 
AGAACTGATG CTTCCTGGGG ACCAGGGTCA GAGACCAACC AGCACATTGG GGCCAGAGTC 
CTAACTATGC CAGCATGCAA GCCTCAGCTA ACCTACACTG CCACACTACC AGAAAGACAC 
CAGGGCCTTC AGGTTCCACA TGCTCAGTCC TGGGGGAATC TTTTCCACTC ACCCTCCCAT 
CCTTCTGCTG TTCACCCAGG GTCCCCACCA TCCAGCAATC TTCATGTACC CCTTAGGTCA 
ACTTGGAATT CTGGTCCTGT TCTGGGGTCC CGAACACCTG GTCCACGAAG AATAGACATG 
CCCCCAGATG ATGACTGGAG ACAGAGCAGT TACCCCTCCC AGGACAGACA CAGGAGCCCA 
GGAGAGGAAA GAATTATGTT TGCTCTATCA AATGCTGCTG GAAGAGAGCA GAACAGGGTG 
AGATTTCTAC AGCACAGCAG GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519768.1
CDS	4253..9019
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_006519705.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519705.4

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCGAAGAA AGGGTCCCTG 
GTGGAGCGCA GGAGGAGCAG TGGCCGTGTT CGGAGAAAAG GCGATGAGCC ACAGGCCTCA 
GGGTACCACA GCGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCTAAA 
TCCAGCAGCA GCAGCAGGCT AAAGGATTTC AAAGAGACAG TCAGCAACAT GATCCACAGC 
AGGCCTTCCC TGGCTTCTCA GACCAGTGCA GGATCTCCCT GTGTAGGTAG GGCAGGAGAC 
CAGCTAGACA AGATACCACC AAGGAACTTC CCTTTACAGG CTCGGGGCTG GGAAACGGAG 
AGTACCAGTA GTGAGGCAAA ATCCAGTTCT TCCAGCAAGT ACCGCCCAAC ATGGAGACCT 
AAACGAGAAT CTCTAAATAT TGACAGCATT TTTAGTAAGG ACAGAAGGAA GCATTGTGGC 
TACACCCAGC TTAGGACCTT TCCTGAAGAT GCAGCTAAAG AATTTACACC GGATGAAGTA 
AGCAAGCCAA CTGCTAATGA CATTAAAGAT GGCGGATCAA GGTCACAGCA TAAACTCTGG 
GGAACAGCTC GGCCAGGCTC TCATCTTTTA GAGCAGCATC CTCGCCTAAT CCAGCGGATG 
GAATCTGGCT ATGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGTTT GGATGCTGCC 
CCGCCTGACA GCGTGAATGT CTACAGGGAT CAAAGCACAA AGAGGCCTGT TGGGTTTGTC 
CCTTCTTGGC GTCATATTCC AAAGTCTCAC AGCAGTAGCA TCCTGGAGGT AGACTGTACA 
GCACCCATGA CCAGCTGGAC AAAGACTCAA CCTCTCTCAG ATGGGGAGGT CACCTCTAGA 
AGTGAATTGG ATGAACTACA GGAAGAAGTG GTCAGGCGAG CCCAGGAACA GGAGCTGCGG 
AAGAAACGGG AGAAGGAATT AGAGGCTGCT AAAGGCTTTA ACCCTCATCC CAGCCGCTAC 
ATGGATTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAAAG GTCCTTGCAA 
GAGGCAAATT CAATATTTGA AGAGTCGCTG CATCTGGAAC AAAAGGGAGA CTGTGCTGCA 
GCTCTGGCTC TGTGTAACGA AGCTATCTCT AAACTAAGAC TTACCCTGCA TGATGCCAGC 
TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGAAGCCTTC AGGATCGCAT GCAGCAACAA GCATCATCGC AGCAGCCGGT GCAGCCCTCA 
GCCTCTCTCC CATCACAGGG GGGCGCCCTC CCTCAGCCGA CAAGTGAACA GCCTATCACG 
CTCCAAGTAT TGTTAAACCA GGAGGCACAG CTGGAACCTT GCAAGGATAC AGAGCTTGGG 
GCCACTTCCT CTTTCTTCCA CTCACCTGCT TCCTGCCCTG AGTTACACTC ATCACTCATC 
CCAGAGTCAC CTGTTTCATC TGTTTCACAG CACAGTCCCC CTGGGACATC TTCCTTGAAG 
CTTCTGACGT CTTTTGAGGT GGACAGCGTC AATCGCTCTG CATTCCACAG GCAGGGTTTA 
CCTAAAGCAA CAGGTAGAAC TGAGATGAAC TCTCAACATG AATGCCTGCC ATTAGATGCC 
CTTGAAGATA GGCTCCAAGG CCACAGGGAG GACAACAGCT GTTGCAGCAA ATTCCCACCA 
CGAGAAGGAA GAGACATAGC TCAAGATCAG CTGTTTGAAG GAAAAAAGAA TCCTGCTGAT 
ATATCCATGG GGATGCCTTG GTCATATTCA ACAGGAGAAG CCACTTCTGA GAGAGTTATA 
TATAGCCTAA ATAGTCCTTC AAGTTCATCA GCTCAGCCTA GTATTCCCCC TTACAGTTCC 
TGCCACCCCA TCACGTCTGC TGCTTCTTCA CCTGTGCTTC ATGCTGCTGA CCCCATGCAG 
AAACTTAACC AACACGTTCA AGCCCAAAGT CTCCAGACTT CATTGACTTC AAAAGTGGTC 
AGAAGCAGTG AGGAGCCCTA TAGGCTAGAG TTCCCCAGTA CAAAAGGACT TGTCCGCTCA 
TTGGCAGAGC AATTCCAGAA GATGCAGAAT ACTTCCACGA GAGATGTTAT AGGTTCCCAG 
GATAGAAGTT TGCCAAATGG TGTCAGGAAG AGCTCTTCTC CTTCTGACTT TATGCCTCCA 
TTATCCCAGG GTCCAGGAAG AGAGCACTGT CGCTGGGTAA AACAACCCCG TTCCCCCGAT 
GGCAGGGAAA GACCGCCCTG CTGGGAAGAC CCTGCAGCTC ATCCTCCTCT TTCCATGGGC 
TCGGGGCTTC CTGATGGTGA AACCTCGAGG ACAGCACAGC CCAGGCTGGC TGAGCCAGAT 
ATGTACCAAG GAAAGCTACC CCAAGTGACA GATATTAGGT CCAAGGAGCT GGGTAGTAGT 
GTCAATTTGG GGACTTCTTT GCCTTTGGAT TCTTGGGTAA ATGTCACAAG GCTCTGTGAT 
TCTCAGGTTA AACCCAGTGC CCCTGGGCCC GGAGACAAGT CCTCCTCCCA TGATTCCCAC 
CCACGTGCAA CCTGCCTAGA AAGAAGTCCT ATCCTTTTGC ATCCACACTG GGACCAAGAC 
ACAGAGCAGG AGACCTCTGA ACTGGAGTCT CTCTACCAAG CCAGCCTTCA GGCCTCTTCT 
CACACTGCCT ATTCTGATTG GAGATCACAG GATGTGGCCT GGCAGCCACT TAGCCTAGCA 
GGCTCTGCAG ATGGCATGGG AAGGAGACTG CACTCAGCCC CTGGGCTTGA TCTCTCAAAG 
CCTCTGACAG CAGAGATGGA GCATGTCCTT TATGAACCGA GCACAGTGCC TGTCTCTCAG 
GATTCATCAA ACGTGAGGAA AAAGACTTTG GAAACCGGGC ACCATTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA TCCTCCTGTA TTTCTCCTTG ATCCTAAACT CTACCCTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCAGCA TGGCAGGGGA GCCCTATAGA 
CCCCCAGGAA CTTCAAGGAG TGTCCACCAG TTTCTGGCTA TGTGTGACAG GGATGAAACT 
CCCCAAGGGG TCAAGTACAC AGGAAGGACT TTGAACTACC GAAGTCTACC TCATCGTTCC 
AGAACTGATG CTTCCTGGGG ACCAGGGTCA GAGACCAACC AGCACATTGG GGCCAGAGTC 
CTAACTATGC CAGCATGCAA GCCTCAGCTA ACCTACACTG CCACACTACC AGAAAGACAC 
CAGGGCCTTC AGGTTCCACA TGCTCAGTCC TGGGGGAATC TTTTCCACTC ACCCTCCCAT 
CCTTCTGCTG TTCACCCAGG GTCCCCACCA TCCAGCAATC TTCATGTACC CCTTAGGTCA 
ACTTGGAATT CTGGTCCTGT TCTGGGGTCC CGAACACCTG GTCCACGAAG AATAGACATG 
CCCCCAGATG ATGACTGGAG ACAGAGCAGT TACCCCTCCC AGGACAGACA CAGGAGCCCA 
GGAGAGGAAA GAATTATGTT TGCTCTATCA AATGCTGCTG GAAGAGAGCA GAACAGGGTG 
AGATTTCTAC AGCACAGCAG GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_011245225.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243527.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X2, mRNA.

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CloneID	OMu50452
Clone ID Related Accession (Same CDS sequence)	XM_006519703.3 , XM_011245225.2 , XM_011245225.3 , XM_030248095.1 , XM_006519703.4
Accession Version	XM_011245225.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245225.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243527.1
CDS	6088..11025
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_011245225.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245225.3

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_030248095.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103955.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X3, mRNA.

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CloneID	OMu50452
Clone ID Related Accession (Same CDS sequence)	XM_006519703.3 , XM_011245225.2 , XM_011245225.3 , XM_030248095.1 , XM_006519703.4
Accession Version	XM_030248095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103955.1
CDS	980..5917
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_030248095.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248095.1

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_006519703.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519766.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X1, mRNA.

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CloneID	OMu50452
Clone ID Related Accession (Same CDS sequence)	XM_006519703.3 , XM_011245225.2 , XM_011245225.3 , XM_030248095.1 , XM_006519703.4
Accession Version	XM_006519703.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519703.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519766.1
CDS	4253..9190
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_006519703.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519703.4

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_011245226.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243528.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X5, mRNA.

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CloneID	OMu71757
Clone ID Related Accession (Same CDS sequence)	XM_011245226.3 , XM_011245226.2
Accession Version	XM_011245226.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTC ACTTCCATAT TGCTGATGAA ACCAAAGAGG ATATATGTAC TGCTCAACAC 
TGCATATCCC ACCAGAAATT TGCAATGACA TTGTTTGAGC AGTGTGTGTG CAGCAGCTGT 
GGCGCCACGT CCGACCCGCT GCCCTTTATC CAGATGGTGC ACTACATCTC CACCACTGCC 
CTCTGTAACC AGGCTATTTG TATGCTGGAA AAGCGAGAGA AACCCTCACC AAGTATGTTT 
GGTGAGCTGC TTCAGAATGC CAGCACCATG GGGGACCTGC GGAACTGTCC GAGCAACTGT 
GGGGAGAGAA TCCGGATCCG CCGTGTCTTG ATGAATGCAC CACAGATTAT CACGATTGGT 
CTGGTTTGGG ACTCAGAGCA TTCAGACTTG GCAGAGGACG TCATCCACAG CCTGGGCACC 
TGCCTTAAGC TGGGTGATCT GTTTTTCAGA GTGACAGATG ACCGGGCCAA GCAGTCTGAA 
CTATACTTAG TAGGAATGAT CTGTTACTAT GGCAAGCATT ATTCTACATT CTTTTTTCAA 
ACAAAGATCC GCAAATGGAT GTATTTTGAT GATGCTCATG TCAAGGAGAT TGGTCCGAAG 
TGGAAGGATG TGGTCACCAA ATGCATTAAA GGGCACTATC AACCCTTGCT GCTGCTCTAT 
GCAGACCCCC AGGGTACTCC AGTGTCTGCC CAGGACCTGC CTCCCCACGC TCAGTTCCTG 
CCATACACCA AGACATGCTA CGATAGTGAA GACTCAGGGA GGGAGCCTTC CATCTCAAGT 
GACACTCGAA CAGATTCCTC AACGGAGAGC TATCCCTACA AACATTTTCA CCTTGAGTCT 
GTGGTCAGTC ACTTCTCTTC AGATTCTCAG GGGACAGTCA TCTATAATGT GGAGAATGAT 
TCCGTGTCTC AGAGCAGTCG GGACACAGGA CACCTGACTG ATAGTGAATG TAATCAGAAA 
CACACATCGA AGAAAGGGTC CCTGGTGGAG CGCAGGAGGA GCAGTGGCCG TGTTCGGAGA 
AAAGGCGATG AGCCACAGGC CTCAGGGTAC CACAGCGAAG GAGAAACACT GAAAGAGAAG 
CAGGCTCCTA GGAATGCCTC TAAATCCAGC AGCAGCAGCA GGCTAAAGGA TTTCAAAGAG 
ACAGTCAGCA ACATGATCCA CAGCAGGCCT TCCCTGGCTT CTCAGACCAG TGCAGGATCT 
CCCTGTGTAG GTAGGGCAGG AGACCAGCTA GACAAGATAC CACCAAGGAA CTTCCCTTTA 
CAGGCTCGGG GCTGGGAAAC GGAGAGTACC AGTAGTGAGG CAAAATCCAG TTCTTCCAGC 
AAGTACCGCC CAACATGGAG ACCTAAACGA GAATCTCTAA ATATTGACAG CATTTTTAGT 
AAGGACAGAA GGAAGCATTG TGGCTACACC CAGCTTAGGA CCTTTCCTGA AGATGCAGCT 
AAAGAATTTA CACCGGATGA AGTAAGCAAG CCAACTGCTA ATGACATTAA AGATGGCGGA 
TCAAGGTCAC AGCATAAACT CTGGGGAACA GCTCGGCCAG GCTCTCATCT TTTAGAGCAG 
CATCCTCGCC TAATCCAGCG GATGGAATCT GGCTATGAGA GCAGTGAGAG GAACAGCAGC 
AGCCCTGTCA GTTTGGATGC TGCCCCGCCT GACAGCGTGA ATGTCTACAG GGATCAAAGC 
ACAAAGAGGC CTGTTGGGTT TGTCCCTTCT TGGCGTCATA TTCCAAAGTC TCACAGCAGT 
AGCATCCTGG AGGTAGACTG TACAGCACCC ATGACCAGCT GGACAAAGAC TCAACCTCTC 
TCAGATGGGG AGGTCACCTC TAGAAGTGAA TTGGATGAAC TACAGGAAGA AGTGGTCAGG 
CGAGCCCAGG AACAGGAGCT GCGGAAGAAA CGGGAGAAGG AATTAGAGGC TGCTAAAGGC 
TTTAACCCTC ATCCCAGCCG CTACATGGAT TTGGATGAAC TGCAGAATCA GGGGAGGAGT 
GACGGCTTTG AAAGGTCCTT GCAAGAGGCA AATTCAATAT TTGAAGAGTC GCTGCATCTG 
GAACAAAAGG GAGACTGTGC TGCAGCTCTG GCTCTGTGTA ACGAAGCTAT CTCTAAACTA 
AGACTTACCC TGCATGATGC CAGCTCTAGC ACGCACAGCA GAGCCCTAGT CGATAAGAAG 
TTGCAAATCA GTATTCGAAA AGCACGAAGC CTTCAGGATC GCATGCAGCA ACAAGCATCA 
TCGCAGCAGC CGGTGCAGCC CTCAGCCTCT CTCCCATCAC AGGGGGGCGC CCTCCCTCAG 
CCGACAAGTG AACAGCCTAT CACGCTCCAA GTATTGTTAA ACCAGGAGGC ACAGCTGGAA 
CCTTGCAAGG ATACAGAGCT TGGGGCCACT TCCTCTTTCT TCCACTCACC TGCTTCCTGC 
CCTGAGTTAC ACTCATCACT CATCCCAGAG TCACCTGTTT CATCTGTTTC ACAGCACAGT 
CCCCCTGGGA CATCTTCCTT GAAGCTTCTG ACGTCTTTTG AGGTGGACAG CGTCAATCGC 
TCTGCATTCC ACAGGCAGGG TTTACCTAAA GCAACAGGTA GAACTGAGAT GAACTCTCAA 
CATGAATGCC TGCCATTAGA TGCCCTTGAA GATAGGCTCC AAGGCCACAG GGAGGACAAC 
AGCTGTTGCA GCAAATTCCC ACCACGAGAA GGAAGAGACA TAGCTCAAGA TCAGCTGTTT 
GAAGGAAAAA AGAATCCTGC TGATATATCC ATGGGGATGC CTTGGTCATA TTCAACAGGA 
GAAGCCACTT CTGAGAGAGT TATATATAGC CTAAATAGTC CTTCAAGTTC ATCAGCTCAG 
CCTAGTATTC CCCCTTACAG TTCCTGCCAC CCCATCACGT CTGCTGCTTC TTCACCTGTG 
CTTCATGCTG CTGACCCCAT GCAGAAACTT AACCAACACG TTCAAGCCCA AAGTCTCCAG 
ACTTCATTGA CTTCAAAAGT GGTCAGAAGC AGTGAGGAGC CCTATAGGCT AGAGTTCCCC 
AGTACAAAAG GACTTGTCCG CTCATTGGCA GAGCAATTCC AGAAGATGCA GAATACTTCC 
ACGAGAGATG TTATAGGTTC CCAGGATAGA AGTTTGCCAA ATGGTGTCAG GAAGAGCTCT 
TCTCCTTCTG ACTTTATGCC TCCATTATCC CAGGGTCCAG GAAGAGAGCA CTGTCGCTGG 
GTAAAACAAC CCCGTTCCCC CGATGGCAGG GAAAGACCGC CCTGCTGGGA AGACCCTGCA 
GCTCATCCTC CTCTTTCCAT GGGCTCGGGG CTTCCTGATG GTGAAACCTC GAGGACAGCA 
CAGCCCAGGC TGGCTGAGCC AGATATGTAC CAAGGAAAGC TACCCCAAGT GACAGATATT 
AGGTCCAAGG AGCTGGGTAG TAGTGTCAAT TTGGGGACTT CTTTGCCTTT GGATTCTTGG 
GTAAATGTCA CAAGGCTCTG TGATTCTCAG GTTAAACCCA GTGCCCCTGG GCCCGGAGAC 
AAGTCCTCCT CCCATGATTC CCACCCACGT GCAACCTGCC TAGAAAGAAG TCCTATCCTT 
TTGCATCCAC ACTGGGACCA AGACACAGAG CAGGAGACCT CTGAACTGGA GTCTCTCTAC 
CAAGCCAGCC TTCAGGCCTC TTCTCACACT GCCTATTCTG ATTGGAGATC ACAGGATGTG 
GCCTGGCAGC CACTTAGCCT AGCAGGCTCT GCAGATGGCA TGGGAAGGAG ACTGCACTCA 
GCCCCTGGGC TTGATCTCTC AAAGCCTCTG ACAGCAGAGA TGGAGCATGT CCTTTATGAA 
CCGAGCACAG TGCCTGTCTC TCAGGATTCA TCAAACGTGA GGAAAAAGAC TTTGGAAACC 
GGGCACCATT GTTCCAGCTC CTCTTCCCTC CCTGTCATCC ATGATCCTCC TGTATTTCTC 
CTTGATCCTA AACTCTACCC TCCCCAACCA CAGTTCCTGT CCCCAGATGT CCTGATGCCC 
AGCATGGCAG GGGAGCCCTA TAGACCCCCA GGAACTTCAA GGAGTGTCCA CCAGTTTCTG 
GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT ACACAGGAAG GACTTTGAAC 
TACCGAAGTC TACCTCATCG TTCCAGAACT GATGCTTCCT GGGGACCAGG GTCAGAGACC 
AACCAGCACA TTGGGGCCAG AGTCCTAACT ATGCCAGCAT GCAAGCCTCA GCTAACCTAC 
ACTGCCACAC TACCAGAAAG ACACCAGGGC CTTCAGGTTC CACATGCTCA GTCCTGGGGG 
AATCTTTTCC ACTCACCCTC CCATCCTTCT GCTGTTCACC CAGGGTCCCC ACCATCCAGC 
AATCTTCATG TACCCCTTAG GTCAACTTGG AATTCTGGTC CTGTTCTGGG GTCCCGAACA 
CCTGGTCCAC GAAGAATAGA CATGCCCCCA GATGATGACT GGAGACAGAG CAGTTACCCC 
TCCCAGGACA GACACAGGAG CCCAGGAGAG GAAAGAATTA TGTTTGCTCT ATCAAATGCT 
GCTGGAAGAG AGCAGAACAG GGTGAGATTT CTACAGCACA GCAGGTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243528.1
CDS	37..4587
Translation	MRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_011245226.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245226.3

ORF Insert Sequence:

ATGAGAATTC ACTTCCATAT TGCTGATGAA ACCAAAGAGG ATATATGTAC TGCTCAACAC 
TGCATATCCC ACCAGAAATT TGCAATGACA TTGTTTGAGC AGTGTGTGTG CAGCAGCTGT 
GGCGCCACGT CCGACCCGCT GCCCTTTATC CAGATGGTGC ACTACATCTC CACCACTGCC 
CTCTGTAACC AGGCTATTTG TATGCTGGAA AAGCGAGAGA AACCCTCACC AAGTATGTTT 
GGTGAGCTGC TTCAGAATGC CAGCACCATG GGGGACCTGC GGAACTGTCC GAGCAACTGT 
GGGGAGAGAA TCCGGATCCG CCGTGTCTTG ATGAATGCAC CACAGATTAT CACGATTGGT 
CTGGTTTGGG ACTCAGAGCA TTCAGACTTG GCAGAGGACG TCATCCACAG CCTGGGCACC 
TGCCTTAAGC TGGGTGATCT GTTTTTCAGA GTGACAGATG ACCGGGCCAA GCAGTCTGAA 
CTATACTTAG TAGGAATGAT CTGTTACTAT GGCAAGCATT ATTCTACATT CTTTTTTCAA 
ACAAAGATCC GCAAATGGAT GTATTTTGAT GATGCTCATG TCAAGGAGAT TGGTCCGAAG 
TGGAAGGATG TGGTCACCAA ATGCATTAAA GGGCACTATC AACCCTTGCT GCTGCTCTAT 
GCAGACCCCC AGGGTACTCC AGTGTCTGCC CAGGACCTGC CTCCCCACGC TCAGTTCCTG 
CCATACACCA AGACATGCTA CGATAGTGAA GACTCAGGGA GGGAGCCTTC CATCTCAAGT 
GACACTCGAA CAGATTCCTC AACGGAGAGC TATCCCTACA AACATTTTCA CCTTGAGTCT 
GTGGTCAGTC ACTTCTCTTC AGATTCTCAG GGGACAGTCA TCTATAATGT GGAGAATGAT 
TCCGTGTCTC AGAGCAGTCG GGACACAGGA CACCTGACTG ATAGTGAATG TAATCAGAAA 
CACACATCGA AGAAAGGGTC CCTGGTGGAG CGCAGGAGGA GCAGTGGCCG TGTTCGGAGA 
AAAGGCGATG AGCCACAGGC CTCAGGGTAC CACAGCGAAG GAGAAACACT GAAAGAGAAG 
CAGGCTCCTA GGAATGCCTC TAAATCCAGC AGCAGCAGCA GGCTAAAGGA TTTCAAAGAG 
ACAGTCAGCA ACATGATCCA CAGCAGGCCT TCCCTGGCTT CTCAGACCAG TGCAGGATCT 
CCCTGTGTAG GTAGGGCAGG AGACCAGCTA GACAAGATAC CACCAAGGAA CTTCCCTTTA 
CAGGCTCGGG GCTGGGAAAC GGAGAGTACC AGTAGTGAGG CAAAATCCAG TTCTTCCAGC 
AAGTACCGCC CAACATGGAG ACCTAAACGA GAATCTCTAA ATATTGACAG CATTTTTAGT 
AAGGACAGAA GGAAGCATTG TGGCTACACC CAGCTTAGGA CCTTTCCTGA AGATGCAGCT 
AAAGAATTTA CACCGGATGA AGTAAGCAAG CCAACTGCTA ATGACATTAA AGATGGCGGA 
TCAAGGTCAC AGCATAAACT CTGGGGAACA GCTCGGCCAG GCTCTCATCT TTTAGAGCAG 
CATCCTCGCC TAATCCAGCG GATGGAATCT GGCTATGAGA GCAGTGAGAG GAACAGCAGC 
AGCCCTGTCA GTTTGGATGC TGCCCCGCCT GACAGCGTGA ATGTCTACAG GGATCAAAGC 
ACAAAGAGGC CTGTTGGGTT TGTCCCTTCT TGGCGTCATA TTCCAAAGTC TCACAGCAGT 
AGCATCCTGG AGGTAGACTG TACAGCACCC ATGACCAGCT GGACAAAGAC TCAACCTCTC 
TCAGATGGGG AGGTCACCTC TAGAAGTGAA TTGGATGAAC TACAGGAAGA AGTGGTCAGG 
CGAGCCCAGG AACAGGAGCT GCGGAAGAAA CGGGAGAAGG AATTAGAGGC TGCTAAAGGC 
TTTAACCCTC ATCCCAGCCG CTACATGGAT TTGGATGAAC TGCAGAATCA GGGGAGGAGT 
GACGGCTTTG AAAGGTCCTT GCAAGAGGCA AATTCAATAT TTGAAGAGTC GCTGCATCTG 
GAACAAAAGG GAGACTGTGC TGCAGCTCTG GCTCTGTGTA ACGAAGCTAT CTCTAAACTA 
AGACTTACCC TGCATGATGC CAGCTCTAGC ACGCACAGCA GAGCCCTAGT CGATAAGAAG 
TTGCAAATCA GTATTCGAAA AGCACGAAGC CTTCAGGATC GCATGCAGCA ACAAGCATCA 
TCGCAGCAGC CGGTGCAGCC CTCAGCCTCT CTCCCATCAC AGGGGGGCGC CCTCCCTCAG 
CCGACAAGTG AACAGCCTAT CACGCTCCAA GTATTGTTAA ACCAGGAGGC ACAGCTGGAA 
CCTTGCAAGG ATACAGAGCT TGGGGCCACT TCCTCTTTCT TCCACTCACC TGCTTCCTGC 
CCTGAGTTAC ACTCATCACT CATCCCAGAG TCACCTGTTT CATCTGTTTC ACAGCACAGT 
CCCCCTGGGA CATCTTCCTT GAAGCTTCTG ACGTCTTTTG AGGTGGACAG CGTCAATCGC 
TCTGCATTCC ACAGGCAGGG TTTACCTAAA GCAACAGGTA GAACTGAGAT GAACTCTCAA 
CATGAATGCC TGCCATTAGA TGCCCTTGAA GATAGGCTCC AAGGCCACAG GGAGGACAAC 
AGCTGTTGCA GCAAATTCCC ACCACGAGAA GGAAGAGACA TAGCTCAAGA TCAGCTGTTT 
GAAGGAAAAA AGAATCCTGC TGATATATCC ATGGGGATGC CTTGGTCATA TTCAACAGGA 
GAAGCCACTT CTGAGAGAGT TATATATAGC CTAAATAGTC CTTCAAGTTC ATCAGCTCAG 
CCTAGTATTC CCCCTTACAG TTCCTGCCAC CCCATCACGT CTGCTGCTTC TTCACCTGTG 
CTTCATGCTG CTGACCCCAT GCAGAAACTT AACCAACACG TTCAAGCCCA AAGTCTCCAG 
ACTTCATTGA CTTCAAAAGT GGTCAGAAGC AGTGAGGAGC CCTATAGGCT AGAGTTCCCC 
AGTACAAAAG GACTTGTCCG CTCATTGGCA GAGCAATTCC AGAAGATGCA GAATACTTCC 
ACGAGAGATG TTATAGGTTC CCAGGATAGA AGTTTGCCAA ATGGTGTCAG GAAGAGCTCT 
TCTCCTTCTG ACTTTATGCC TCCATTATCC CAGGGTCCAG GAAGAGAGCA CTGTCGCTGG 
GTAAAACAAC CCCGTTCCCC CGATGGCAGG GAAAGACCGC CCTGCTGGGA AGACCCTGCA 
GCTCATCCTC CTCTTTCCAT GGGCTCGGGG CTTCCTGATG GTGAAACCTC GAGGACAGCA 
CAGCCCAGGC TGGCTGAGCC AGATATGTAC CAAGGAAAGC TACCCCAAGT GACAGATATT 
AGGTCCAAGG AGCTGGGTAG TAGTGTCAAT TTGGGGACTT CTTTGCCTTT GGATTCTTGG 
GTAAATGTCA CAAGGCTCTG TGATTCTCAG GTTAAACCCA GTGCCCCTGG GCCCGGAGAC 
AAGTCCTCCT CCCATGATTC CCACCCACGT GCAACCTGCC TAGAAAGAAG TCCTATCCTT 
TTGCATCCAC ACTGGGACCA AGACACAGAG CAGGAGACCT CTGAACTGGA GTCTCTCTAC 
CAAGCCAGCC TTCAGGCCTC TTCTCACACT GCCTATTCTG ATTGGAGATC ACAGGATGTG 
GCCTGGCAGC CACTTAGCCT AGCAGGCTCT GCAGATGGCA TGGGAAGGAG ACTGCACTCA 
GCCCCTGGGC TTGATCTCTC AAAGCCTCTG ACAGCAGAGA TGGAGCATGT CCTTTATGAA 
CCGAGCACAG TGCCTGTCTC TCAGGATTCA TCAAACGTGA GGAAAAAGAC TTTGGAAACC 
GGGCACCATT GTTCCAGCTC CTCTTCCCTC CCTGTCATCC ATGATCCTCC TGTATTTCTC 
CTTGATCCTA AACTCTACCC TCCCCAACCA CAGTTCCTGT CCCCAGATGT CCTGATGCCC 
AGCATGGCAG GGGAGCCCTA TAGACCCCCA GGAACTTCAA GGAGTGTCCA CCAGTTTCTG 
GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT ACACAGGAAG GACTTTGAAC 
TACCGAAGTC TACCTCATCG TTCCAGAACT GATGCTTCCT GGGGACCAGG GTCAGAGACC 
AACCAGCACA TTGGGGCCAG AGTCCTAACT ATGCCAGCAT GCAAGCCTCA GCTAACCTAC 
ACTGCCACAC TACCAGAAAG ACACCAGGGC CTTCAGGTTC CACATGCTCA GTCCTGGGGG 
AATCTTTTCC ACTCACCCTC CCATCCTTCT GCTGTTCACC CAGGGTCCCC ACCATCCAGC 
AATCTTCATG TACCCCTTAG GTCAACTTGG AATTCTGGTC CTGTTCTGGG GTCCCGAACA 
CCTGGTCCAC GAAGAATAGA CATGCCCCCA GATGATGACT GGAGACAGAG CAGTTACCCC 
TCCCAGGACA GACACAGGAG CCCAGGAGAG GAAAGAATTA TGTTTGCTCT ATCAAATGCT 
GCTGGAAGAG AGCAGAACAG GGTGAGATTT CTACAGCACA GCAGGTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( NM_030180.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084456.2 Mus musculus ubiquitin specific peptidase 54 (Usp54), mRNA.

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CloneID	OMu08033
Clone ID Related Accession (Same CDS sequence)	NM_030180.2 , XM_006519705.4 , XM_006519705.3
Accession Version	NM_030180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK047620.1, AC146594.3, CF741928.1, CF535703.1, CK638913.1 and BC117845.1. On Jan 24, 2007 this sequence version replaced NM_030180.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCGAAGAA AGGGTCCCTG 
GTGGAGCGCA GGAGGAGCAG TGGCCGTGTT CGGAGAAAAG GCGATGAGCC ACAGGCCTCA 
GGGTACCACA GCGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCTAAA 
TCCAGCAGCA GCAGCAGGCT AAAGGATTTC AAAGAGACAG TCAGCAACAT GATCCACAGC 
AGGCCTTCCC TGGCTTCTCA GACCAGTGCA GGATCTCCCT GTGTAGGTAG GGCAGGAGAC 
CAGCTAGACA AGATACCACC AAGGAACTTC CCTTTACAGG CTCGGGGCTG GGAAACGGAG 
AGTACCAGTA GTGAGGCAAA ATCCAGTTCT TCCAGCAAGT ACCGCCCAAC ATGGAGACCT 
AAACGAGAAT CTCTAAATAT TGACAGCATT TTTAGTAAGG ACAGAAGGAA GCATTGTGGC 
TACACCCAGC TTAGGACCTT TCCTGAAGAT GCAGCTAAAG AATTTACACC GGATGAAGTA 
AGCAAGCCAA CTGCTAATGA CATTAAAGAT GGCGGATCAA GGTCACAGCA TAAACTCTGG 
GGAACAGCTC GGCCAGGCTC TCATCTTTTA GAGCAGCATC CTCGCCTAAT CCAGCGGATG 
GAATCTGGCT ATGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGTTT GGATGCTGCC 
CCGCCTGACA GCGTGAATGT CTACAGGGAT CAAAGCACAA AGAGGCCTGT TGGGTTTGTC 
CCTTCTTGGC GTCATATTCC AAAGTCTCAC AGCAGTAGCA TCCTGGAGGT AGACTGTACA 
GCACCCATGA CCAGCTGGAC AAAGACTCAA CCTCTCTCAG ATGGGGAGGT CACCTCTAGA 
AGTGAATTGG ATGAACTACA GGAAGAAGTG GTCAGGCGAG CCCAGGAACA GGAGCTGCGG 
AAGAAACGGG AGAAGGAATT AGAGGCTGCT AAAGGCTTTA ACCCTCATCC CAGCCGCTAC 
ATGGATTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAAAG GTCCTTGCAA 
GAGGCAAATT CAATATTTGA AGAGTCGCTG CATCTGGAAC AAAAGGGAGA CTGTGCTGCA 
GCTCTGGCTC TGTGTAACGA AGCTATCTCT AAACTAAGAC TTACCCTGCA TGATGCCAGC 
TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGAAGCCTTC AGGATCGCAT GCAGCAACAA GCATCATCGC AGCAGCCGGT GCAGCCCTCA 
GCCTCTCTCC CATCACAGGG GGGCGCCCTC CCTCAGCCGA CAAGTGAACA GCCTATCACG 
CTCCAAGTAT TGTTAAACCA GGAGGCACAG CTGGAACCTT GCAAGGATAC AGAGCTTGGG 
GCCACTTCCT CTTTCTTCCA CTCACCTGCT TCCTGCCCTG AGTTACACTC ATCACTCATC 
CCAGAGTCAC CTGTTTCATC TGTTTCACAG CACAGTCCCC CTGGGACATC TTCCTTGAAG 
CTTCTGACGT CTTTTGAGGT GGACAGCGTC AATCGCTCTG CATTCCACAG GCAGGGTTTA 
CCTAAAGCAA CAGGTAGAAC TGAGATGAAC TCTCAACATG AATGCCTGCC ATTAGATGCC 
CTTGAAGATA GGCTCCAAGG CCACAGGGAG GACAACAGCT GTTGCAGCAA ATTCCCACCA 
CGAGAAGGAA GAGACATAGC TCAAGATCAG CTGTTTGAAG GAAAAAAGAA TCCTGCTGAT 
ATATCCATGG GGATGCCTTG GTCATATTCA ACAGGAGAAG CCACTTCTGA GAGAGTTATA 
TATAGCCTAA ATAGTCCTTC AAGTTCATCA GCTCAGCCTA GTATTCCCCC TTACAGTTCC 
TGCCACCCCA TCACGTCTGC TGCTTCTTCA CCTGTGCTTC ATGCTGCTGA CCCCATGCAG 
AAACTTAACC AACACGTTCA AGCCCAAAGT CTCCAGACTT CATTGACTTC AAAAGTGGTC 
AGAAGCAGTG AGGAGCCCTA TAGGCTAGAG TTCCCCAGTA CAAAAGGACT TGTCCGCTCA 
TTGGCAGAGC AATTCCAGAA GATGCAGAAT ACTTCCACGA GAGATGTTAT AGGTTCCCAG 
GATAGAAGTT TGCCAAATGG TGTCAGGAAG AGCTCTTCTC CTTCTGACTT TATGCCTCCA 
TTATCCCAGG GTCCAGGAAG AGAGCACTGT CGCTGGGTAA AACAACCCCG TTCCCCCGAT 
GGCAGGGAAA GACCGCCCTG CTGGGAAGAC CCTGCAGCTC ATCCTCCTCT TTCCATGGGC 
TCGGGGCTTC CTGATGGTGA AACCTCGAGG ACAGCACAGC CCAGGCTGGC TGAGCCAGAT 
ATGTACCAAG GAAAGCTACC CCAAGTGACA GATATTAGGT CCAAGGAGCT GGGTAGTAGT 
GTCAATTTGG GGACTTCTTT GCCTTTGGAT TCTTGGGTAA ATGTCACAAG GCTCTGTGAT 
TCTCAGGTTA AACCCAGTGC CCCTGGGCCC GGAGACAAGT CCTCCTCCCA TGATTCCCAC 
CCACGTGCAA CCTGCCTAGA AAGAAGTCCT ATCCTTTTGC ATCCACACTG GGACCAAGAC 
ACAGAGCAGG AGACCTCTGA ACTGGAGTCT CTCTACCAAG CCAGCCTTCA GGCCTCTTCT 
CACACTGCCT ATTCTGATTG GAGATCACAG GATGTGGCCT GGCAGCCACT TAGCCTAGCA 
GGCTCTGCAG ATGGCATGGG AAGGAGACTG CACTCAGCCC CTGGGCTTGA TCTCTCAAAG 
CCTCTGACAG CAGAGATGGA GCATGTCCTT TATGAACCGA GCACAGTGCC TGTCTCTCAG 
GATTCATCAA ACGTGAGGAA AAAGACTTTG GAAACCGGGC ACCATTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA TCCTCCTGTA TTTCTCCTTG ATCCTAAACT CTACCCTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCAGCA TGGCAGGGGA GCCCTATAGA 
CCCCCAGGAA CTTCAAGGAG TGTCCACCAG TTTCTGGCTA TGTGTGACAG GGATGAAACT 
CCCCAAGGGG TCAAGTACAC AGGAAGGACT TTGAACTACC GAAGTCTACC TCATCGTTCC 
AGAACTGATG CTTCCTGGGG ACCAGGGTCA GAGACCAACC AGCACATTGG GGCCAGAGTC 
CTAACTATGC CAGCATGCAA GCCTCAGCTA ACCTACACTG CCACACTACC AGAAAGACAC 
CAGGGCCTTC AGGTTCCACA TGCTCAGTCC TGGGGGAATC TTTTCCACTC ACCCTCCCAT 
CCTTCTGCTG TTCACCCAGG GTCCCCACCA TCCAGCAATC TTCATGTACC CCTTAGGTCA 
ACTTGGAATT CTGGTCCTGT TCTGGGGTCC CGAACACCTG GTCCACGAAG AATAGACATG 
CCCCCAGATG ATGACTGGAG ACAGAGCAGT TACCCCTCCC AGGACAGACA CAGGAGCCCA 
GGAGAGGAAA GAATTATGTT TGCTCTATCA AATGCTGCTG GAAGAGAGCA GAACAGGGTG 
AGATTTCTAC AGCACAGCAG GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084456.2
CDS	740..5506
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	NM_030180.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030180.2

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCGAAGAA AGGGTCCCTG 
GTGGAGCGCA GGAGGAGCAG TGGCCGTGTT CGGAGAAAAG GCGATGAGCC ACAGGCCTCA 
GGGTACCACA GCGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCTAAA 
TCCAGCAGCA GCAGCAGGCT AAAGGATTTC AAAGAGACAG TCAGCAACAT GATCCACAGC 
AGGCCTTCCC TGGCTTCTCA GACCAGTGCA GGATCTCCCT GTGTAGGTAG GGCAGGAGAC 
CAGCTAGACA AGATACCACC AAGGAACTTC CCTTTACAGG CTCGGGGCTG GGAAACGGAG 
AGTACCAGTA GTGAGGCAAA ATCCAGTTCT TCCAGCAAGT ACCGCCCAAC ATGGAGACCT 
AAACGAGAAT CTCTAAATAT TGACAGCATT TTTAGTAAGG ACAGAAGGAA GCATTGTGGC 
TACACCCAGC TTAGGACCTT TCCTGAAGAT GCAGCTAAAG AATTTACACC GGATGAAGTA 
AGCAAGCCAA CTGCTAATGA CATTAAAGAT GGCGGATCAA GGTCACAGCA TAAACTCTGG 
GGAACAGCTC GGCCAGGCTC TCATCTTTTA GAGCAGCATC CTCGCCTAAT CCAGCGGATG 
GAATCTGGCT ATGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGTTT GGATGCTGCC 
CCGCCTGACA GCGTGAATGT CTACAGGGAT CAAAGCACAA AGAGGCCTGT TGGGTTTGTC 
CCTTCTTGGC GTCATATTCC AAAGTCTCAC AGCAGTAGCA TCCTGGAGGT AGACTGTACA 
GCACCCATGA CCAGCTGGAC AAAGACTCAA CCTCTCTCAG ATGGGGAGGT CACCTCTAGA 
AGTGAATTGG ATGAACTACA GGAAGAAGTG GTCAGGCGAG CCCAGGAACA GGAGCTGCGG 
AAGAAACGGG AGAAGGAATT AGAGGCTGCT AAAGGCTTTA ACCCTCATCC CAGCCGCTAC 
ATGGATTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAAAG GTCCTTGCAA 
GAGGCAAATT CAATATTTGA AGAGTCGCTG CATCTGGAAC AAAAGGGAGA CTGTGCTGCA 
GCTCTGGCTC TGTGTAACGA AGCTATCTCT AAACTAAGAC TTACCCTGCA TGATGCCAGC 
TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGAAGCCTTC AGGATCGCAT GCAGCAACAA GCATCATCGC AGCAGCCGGT GCAGCCCTCA 
GCCTCTCTCC CATCACAGGG GGGCGCCCTC CCTCAGCCGA CAAGTGAACA GCCTATCACG 
CTCCAAGTAT TGTTAAACCA GGAGGCACAG CTGGAACCTT GCAAGGATAC AGAGCTTGGG 
GCCACTTCCT CTTTCTTCCA CTCACCTGCT TCCTGCCCTG AGTTACACTC ATCACTCATC 
CCAGAGTCAC CTGTTTCATC TGTTTCACAG CACAGTCCCC CTGGGACATC TTCCTTGAAG 
CTTCTGACGT CTTTTGAGGT GGACAGCGTC AATCGCTCTG CATTCCACAG GCAGGGTTTA 
CCTAAAGCAA CAGGTAGAAC TGAGATGAAC TCTCAACATG AATGCCTGCC ATTAGATGCC 
CTTGAAGATA GGCTCCAAGG CCACAGGGAG GACAACAGCT GTTGCAGCAA ATTCCCACCA 
CGAGAAGGAA GAGACATAGC TCAAGATCAG CTGTTTGAAG GAAAAAAGAA TCCTGCTGAT 
ATATCCATGG GGATGCCTTG GTCATATTCA ACAGGAGAAG CCACTTCTGA GAGAGTTATA 
TATAGCCTAA ATAGTCCTTC AAGTTCATCA GCTCAGCCTA GTATTCCCCC TTACAGTTCC 
TGCCACCCCA TCACGTCTGC TGCTTCTTCA CCTGTGCTTC ATGCTGCTGA CCCCATGCAG 
AAACTTAACC AACACGTTCA AGCCCAAAGT CTCCAGACTT CATTGACTTC AAAAGTGGTC 
AGAAGCAGTG AGGAGCCCTA TAGGCTAGAG TTCCCCAGTA CAAAAGGACT TGTCCGCTCA 
TTGGCAGAGC AATTCCAGAA GATGCAGAAT ACTTCCACGA GAGATGTTAT AGGTTCCCAG 
GATAGAAGTT TGCCAAATGG TGTCAGGAAG AGCTCTTCTC CTTCTGACTT TATGCCTCCA 
TTATCCCAGG GTCCAGGAAG AGAGCACTGT CGCTGGGTAA AACAACCCCG TTCCCCCGAT 
GGCAGGGAAA GACCGCCCTG CTGGGAAGAC CCTGCAGCTC ATCCTCCTCT TTCCATGGGC 
TCGGGGCTTC CTGATGGTGA AACCTCGAGG ACAGCACAGC CCAGGCTGGC TGAGCCAGAT 
ATGTACCAAG GAAAGCTACC CCAAGTGACA GATATTAGGT CCAAGGAGCT GGGTAGTAGT 
GTCAATTTGG GGACTTCTTT GCCTTTGGAT TCTTGGGTAA ATGTCACAAG GCTCTGTGAT 
TCTCAGGTTA AACCCAGTGC CCCTGGGCCC GGAGACAAGT CCTCCTCCCA TGATTCCCAC 
CCACGTGCAA CCTGCCTAGA AAGAAGTCCT ATCCTTTTGC ATCCACACTG GGACCAAGAC 
ACAGAGCAGG AGACCTCTGA ACTGGAGTCT CTCTACCAAG CCAGCCTTCA GGCCTCTTCT 
CACACTGCCT ATTCTGATTG GAGATCACAG GATGTGGCCT GGCAGCCACT TAGCCTAGCA 
GGCTCTGCAG ATGGCATGGG AAGGAGACTG CACTCAGCCC CTGGGCTTGA TCTCTCAAAG 
CCTCTGACAG CAGAGATGGA GCATGTCCTT TATGAACCGA GCACAGTGCC TGTCTCTCAG 
GATTCATCAA ACGTGAGGAA AAAGACTTTG GAAACCGGGC ACCATTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA TCCTCCTGTA TTTCTCCTTG ATCCTAAACT CTACCCTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCAGCA TGGCAGGGGA GCCCTATAGA 
CCCCCAGGAA CTTCAAGGAG TGTCCACCAG TTTCTGGCTA TGTGTGACAG GGATGAAACT 
CCCCAAGGGG TCAAGTACAC AGGAAGGACT TTGAACTACC GAAGTCTACC TCATCGTTCC 
AGAACTGATG CTTCCTGGGG ACCAGGGTCA GAGACCAACC AGCACATTGG GGCCAGAGTC 
CTAACTATGC CAGCATGCAA GCCTCAGCTA ACCTACACTG CCACACTACC AGAAAGACAC 
CAGGGCCTTC AGGTTCCACA TGCTCAGTCC TGGGGGAATC TTTTCCACTC ACCCTCCCAT 
CCTTCTGCTG TTCACCCAGG GTCCCCACCA TCCAGCAATC TTCATGTACC CCTTAGGTCA 
ACTTGGAATT CTGGTCCTGT TCTGGGGTCC CGAACACCTG GTCCACGAAG AATAGACATG 
CCCCCAGATG ATGACTGGAG ACAGAGCAGT TACCCCTCCC AGGACAGACA CAGGAGCCCA 
GGAGAGGAAA GAATTATGTT TGCTCTATCA AATGCTGCTG GAAGAGAGCA GAACAGGGTG 
AGATTTCTAC AGCACAGCAG GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_006519705.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519768.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu08033
Clone ID Related Accession (Same CDS sequence)	NM_030180.2 , XM_006519705.4 , XM_006519705.3
Accession Version	XM_006519705.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCGAAGAA AGGGTCCCTG 
GTGGAGCGCA GGAGGAGCAG TGGCCGTGTT CGGAGAAAAG GCGATGAGCC ACAGGCCTCA 
GGGTACCACA GCGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCTAAA 
TCCAGCAGCA GCAGCAGGCT AAAGGATTTC AAAGAGACAG TCAGCAACAT GATCCACAGC 
AGGCCTTCCC TGGCTTCTCA GACCAGTGCA GGATCTCCCT GTGTAGGTAG GGCAGGAGAC 
CAGCTAGACA AGATACCACC AAGGAACTTC CCTTTACAGG CTCGGGGCTG GGAAACGGAG 
AGTACCAGTA GTGAGGCAAA ATCCAGTTCT TCCAGCAAGT ACCGCCCAAC ATGGAGACCT 
AAACGAGAAT CTCTAAATAT TGACAGCATT TTTAGTAAGG ACAGAAGGAA GCATTGTGGC 
TACACCCAGC TTAGGACCTT TCCTGAAGAT GCAGCTAAAG AATTTACACC GGATGAAGTA 
AGCAAGCCAA CTGCTAATGA CATTAAAGAT GGCGGATCAA GGTCACAGCA TAAACTCTGG 
GGAACAGCTC GGCCAGGCTC TCATCTTTTA GAGCAGCATC CTCGCCTAAT CCAGCGGATG 
GAATCTGGCT ATGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGTTT GGATGCTGCC 
CCGCCTGACA GCGTGAATGT CTACAGGGAT CAAAGCACAA AGAGGCCTGT TGGGTTTGTC 
CCTTCTTGGC GTCATATTCC AAAGTCTCAC AGCAGTAGCA TCCTGGAGGT AGACTGTACA 
GCACCCATGA CCAGCTGGAC AAAGACTCAA CCTCTCTCAG ATGGGGAGGT CACCTCTAGA 
AGTGAATTGG ATGAACTACA GGAAGAAGTG GTCAGGCGAG CCCAGGAACA GGAGCTGCGG 
AAGAAACGGG AGAAGGAATT AGAGGCTGCT AAAGGCTTTA ACCCTCATCC CAGCCGCTAC 
ATGGATTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAAAG GTCCTTGCAA 
GAGGCAAATT CAATATTTGA AGAGTCGCTG CATCTGGAAC AAAAGGGAGA CTGTGCTGCA 
GCTCTGGCTC TGTGTAACGA AGCTATCTCT AAACTAAGAC TTACCCTGCA TGATGCCAGC 
TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGAAGCCTTC AGGATCGCAT GCAGCAACAA GCATCATCGC AGCAGCCGGT GCAGCCCTCA 
GCCTCTCTCC CATCACAGGG GGGCGCCCTC CCTCAGCCGA CAAGTGAACA GCCTATCACG 
CTCCAAGTAT TGTTAAACCA GGAGGCACAG CTGGAACCTT GCAAGGATAC AGAGCTTGGG 
GCCACTTCCT CTTTCTTCCA CTCACCTGCT TCCTGCCCTG AGTTACACTC ATCACTCATC 
CCAGAGTCAC CTGTTTCATC TGTTTCACAG CACAGTCCCC CTGGGACATC TTCCTTGAAG 
CTTCTGACGT CTTTTGAGGT GGACAGCGTC AATCGCTCTG CATTCCACAG GCAGGGTTTA 
CCTAAAGCAA CAGGTAGAAC TGAGATGAAC TCTCAACATG AATGCCTGCC ATTAGATGCC 
CTTGAAGATA GGCTCCAAGG CCACAGGGAG GACAACAGCT GTTGCAGCAA ATTCCCACCA 
CGAGAAGGAA GAGACATAGC TCAAGATCAG CTGTTTGAAG GAAAAAAGAA TCCTGCTGAT 
ATATCCATGG GGATGCCTTG GTCATATTCA ACAGGAGAAG CCACTTCTGA GAGAGTTATA 
TATAGCCTAA ATAGTCCTTC AAGTTCATCA GCTCAGCCTA GTATTCCCCC TTACAGTTCC 
TGCCACCCCA TCACGTCTGC TGCTTCTTCA CCTGTGCTTC ATGCTGCTGA CCCCATGCAG 
AAACTTAACC AACACGTTCA AGCCCAAAGT CTCCAGACTT CATTGACTTC AAAAGTGGTC 
AGAAGCAGTG AGGAGCCCTA TAGGCTAGAG TTCCCCAGTA CAAAAGGACT TGTCCGCTCA 
TTGGCAGAGC AATTCCAGAA GATGCAGAAT ACTTCCACGA GAGATGTTAT AGGTTCCCAG 
GATAGAAGTT TGCCAAATGG TGTCAGGAAG AGCTCTTCTC CTTCTGACTT TATGCCTCCA 
TTATCCCAGG GTCCAGGAAG AGAGCACTGT CGCTGGGTAA AACAACCCCG TTCCCCCGAT 
GGCAGGGAAA GACCGCCCTG CTGGGAAGAC CCTGCAGCTC ATCCTCCTCT TTCCATGGGC 
TCGGGGCTTC CTGATGGTGA AACCTCGAGG ACAGCACAGC CCAGGCTGGC TGAGCCAGAT 
ATGTACCAAG GAAAGCTACC CCAAGTGACA GATATTAGGT CCAAGGAGCT GGGTAGTAGT 
GTCAATTTGG GGACTTCTTT GCCTTTGGAT TCTTGGGTAA ATGTCACAAG GCTCTGTGAT 
TCTCAGGTTA AACCCAGTGC CCCTGGGCCC GGAGACAAGT CCTCCTCCCA TGATTCCCAC 
CCACGTGCAA CCTGCCTAGA AAGAAGTCCT ATCCTTTTGC ATCCACACTG GGACCAAGAC 
ACAGAGCAGG AGACCTCTGA ACTGGAGTCT CTCTACCAAG CCAGCCTTCA GGCCTCTTCT 
CACACTGCCT ATTCTGATTG GAGATCACAG GATGTGGCCT GGCAGCCACT TAGCCTAGCA 
GGCTCTGCAG ATGGCATGGG AAGGAGACTG CACTCAGCCC CTGGGCTTGA TCTCTCAAAG 
CCTCTGACAG CAGAGATGGA GCATGTCCTT TATGAACCGA GCACAGTGCC TGTCTCTCAG 
GATTCATCAA ACGTGAGGAA AAAGACTTTG GAAACCGGGC ACCATTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA TCCTCCTGTA TTTCTCCTTG ATCCTAAACT CTACCCTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCAGCA TGGCAGGGGA GCCCTATAGA 
CCCCCAGGAA CTTCAAGGAG TGTCCACCAG TTTCTGGCTA TGTGTGACAG GGATGAAACT 
CCCCAAGGGG TCAAGTACAC AGGAAGGACT TTGAACTACC GAAGTCTACC TCATCGTTCC 
AGAACTGATG CTTCCTGGGG ACCAGGGTCA GAGACCAACC AGCACATTGG GGCCAGAGTC 
CTAACTATGC CAGCATGCAA GCCTCAGCTA ACCTACACTG CCACACTACC AGAAAGACAC 
CAGGGCCTTC AGGTTCCACA TGCTCAGTCC TGGGGGAATC TTTTCCACTC ACCCTCCCAT 
CCTTCTGCTG TTCACCCAGG GTCCCCACCA TCCAGCAATC TTCATGTACC CCTTAGGTCA 
ACTTGGAATT CTGGTCCTGT TCTGGGGTCC CGAACACCTG GTCCACGAAG AATAGACATG 
CCCCCAGATG ATGACTGGAG ACAGAGCAGT TACCCCTCCC AGGACAGACA CAGGAGCCCA 
GGAGAGGAAA GAATTATGTT TGCTCTATCA AATGCTGCTG GAAGAGAGCA GAACAGGGTG 
AGATTTCTAC AGCACAGCAG GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519768.1
CDS	1000..5766
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_006519705.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519705.3

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGACACC TGACTGATAG TGAATGTAAT CAGAAACACA CATCGAAGAA AGGGTCCCTG 
GTGGAGCGCA GGAGGAGCAG TGGCCGTGTT CGGAGAAAAG GCGATGAGCC ACAGGCCTCA 
GGGTACCACA GCGAAGGAGA AACACTGAAA GAGAAGCAGG CTCCTAGGAA TGCCTCTAAA 
TCCAGCAGCA GCAGCAGGCT AAAGGATTTC AAAGAGACAG TCAGCAACAT GATCCACAGC 
AGGCCTTCCC TGGCTTCTCA GACCAGTGCA GGATCTCCCT GTGTAGGTAG GGCAGGAGAC 
CAGCTAGACA AGATACCACC AAGGAACTTC CCTTTACAGG CTCGGGGCTG GGAAACGGAG 
AGTACCAGTA GTGAGGCAAA ATCCAGTTCT TCCAGCAAGT ACCGCCCAAC ATGGAGACCT 
AAACGAGAAT CTCTAAATAT TGACAGCATT TTTAGTAAGG ACAGAAGGAA GCATTGTGGC 
TACACCCAGC TTAGGACCTT TCCTGAAGAT GCAGCTAAAG AATTTACACC GGATGAAGTA 
AGCAAGCCAA CTGCTAATGA CATTAAAGAT GGCGGATCAA GGTCACAGCA TAAACTCTGG 
GGAACAGCTC GGCCAGGCTC TCATCTTTTA GAGCAGCATC CTCGCCTAAT CCAGCGGATG 
GAATCTGGCT ATGAGAGCAG TGAGAGGAAC AGCAGCAGCC CTGTCAGTTT GGATGCTGCC 
CCGCCTGACA GCGTGAATGT CTACAGGGAT CAAAGCACAA AGAGGCCTGT TGGGTTTGTC 
CCTTCTTGGC GTCATATTCC AAAGTCTCAC AGCAGTAGCA TCCTGGAGGT AGACTGTACA 
GCACCCATGA CCAGCTGGAC AAAGACTCAA CCTCTCTCAG ATGGGGAGGT CACCTCTAGA 
AGTGAATTGG ATGAACTACA GGAAGAAGTG GTCAGGCGAG CCCAGGAACA GGAGCTGCGG 
AAGAAACGGG AGAAGGAATT AGAGGCTGCT AAAGGCTTTA ACCCTCATCC CAGCCGCTAC 
ATGGATTTGG ATGAACTGCA GAATCAGGGG AGGAGTGACG GCTTTGAAAG GTCCTTGCAA 
GAGGCAAATT CAATATTTGA AGAGTCGCTG CATCTGGAAC AAAAGGGAGA CTGTGCTGCA 
GCTCTGGCTC TGTGTAACGA AGCTATCTCT AAACTAAGAC TTACCCTGCA TGATGCCAGC 
TCTAGCACGC ACAGCAGAGC CCTAGTCGAT AAGAAGTTGC AAATCAGTAT TCGAAAAGCA 
CGAAGCCTTC AGGATCGCAT GCAGCAACAA GCATCATCGC AGCAGCCGGT GCAGCCCTCA 
GCCTCTCTCC CATCACAGGG GGGCGCCCTC CCTCAGCCGA CAAGTGAACA GCCTATCACG 
CTCCAAGTAT TGTTAAACCA GGAGGCACAG CTGGAACCTT GCAAGGATAC AGAGCTTGGG 
GCCACTTCCT CTTTCTTCCA CTCACCTGCT TCCTGCCCTG AGTTACACTC ATCACTCATC 
CCAGAGTCAC CTGTTTCATC TGTTTCACAG CACAGTCCCC CTGGGACATC TTCCTTGAAG 
CTTCTGACGT CTTTTGAGGT GGACAGCGTC AATCGCTCTG CATTCCACAG GCAGGGTTTA 
CCTAAAGCAA CAGGTAGAAC TGAGATGAAC TCTCAACATG AATGCCTGCC ATTAGATGCC 
CTTGAAGATA GGCTCCAAGG CCACAGGGAG GACAACAGCT GTTGCAGCAA ATTCCCACCA 
CGAGAAGGAA GAGACATAGC TCAAGATCAG CTGTTTGAAG GAAAAAAGAA TCCTGCTGAT 
ATATCCATGG GGATGCCTTG GTCATATTCA ACAGGAGAAG CCACTTCTGA GAGAGTTATA 
TATAGCCTAA ATAGTCCTTC AAGTTCATCA GCTCAGCCTA GTATTCCCCC TTACAGTTCC 
TGCCACCCCA TCACGTCTGC TGCTTCTTCA CCTGTGCTTC ATGCTGCTGA CCCCATGCAG 
AAACTTAACC AACACGTTCA AGCCCAAAGT CTCCAGACTT CATTGACTTC AAAAGTGGTC 
AGAAGCAGTG AGGAGCCCTA TAGGCTAGAG TTCCCCAGTA CAAAAGGACT TGTCCGCTCA 
TTGGCAGAGC AATTCCAGAA GATGCAGAAT ACTTCCACGA GAGATGTTAT AGGTTCCCAG 
GATAGAAGTT TGCCAAATGG TGTCAGGAAG AGCTCTTCTC CTTCTGACTT TATGCCTCCA 
TTATCCCAGG GTCCAGGAAG AGAGCACTGT CGCTGGGTAA AACAACCCCG TTCCCCCGAT 
GGCAGGGAAA GACCGCCCTG CTGGGAAGAC CCTGCAGCTC ATCCTCCTCT TTCCATGGGC 
TCGGGGCTTC CTGATGGTGA AACCTCGAGG ACAGCACAGC CCAGGCTGGC TGAGCCAGAT 
ATGTACCAAG GAAAGCTACC CCAAGTGACA GATATTAGGT CCAAGGAGCT GGGTAGTAGT 
GTCAATTTGG GGACTTCTTT GCCTTTGGAT TCTTGGGTAA ATGTCACAAG GCTCTGTGAT 
TCTCAGGTTA AACCCAGTGC CCCTGGGCCC GGAGACAAGT CCTCCTCCCA TGATTCCCAC 
CCACGTGCAA CCTGCCTAGA AAGAAGTCCT ATCCTTTTGC ATCCACACTG GGACCAAGAC 
ACAGAGCAGG AGACCTCTGA ACTGGAGTCT CTCTACCAAG CCAGCCTTCA GGCCTCTTCT 
CACACTGCCT ATTCTGATTG GAGATCACAG GATGTGGCCT GGCAGCCACT TAGCCTAGCA 
GGCTCTGCAG ATGGCATGGG AAGGAGACTG CACTCAGCCC CTGGGCTTGA TCTCTCAAAG 
CCTCTGACAG CAGAGATGGA GCATGTCCTT TATGAACCGA GCACAGTGCC TGTCTCTCAG 
GATTCATCAA ACGTGAGGAA AAAGACTTTG GAAACCGGGC ACCATTGTTC CAGCTCCTCT 
TCCCTCCCTG TCATCCATGA TCCTCCTGTA TTTCTCCTTG ATCCTAAACT CTACCCTCCC 
CAACCACAGT TCCTGTCCCC AGATGTCCTG ATGCCCAGCA TGGCAGGGGA GCCCTATAGA 
CCCCCAGGAA CTTCAAGGAG TGTCCACCAG TTTCTGGCTA TGTGTGACAG GGATGAAACT 
CCCCAAGGGG TCAAGTACAC AGGAAGGACT TTGAACTACC GAAGTCTACC TCATCGTTCC 
AGAACTGATG CTTCCTGGGG ACCAGGGTCA GAGACCAACC AGCACATTGG GGCCAGAGTC 
CTAACTATGC CAGCATGCAA GCCTCAGCTA ACCTACACTG CCACACTACC AGAAAGACAC 
CAGGGCCTTC AGGTTCCACA TGCTCAGTCC TGGGGGAATC TTTTCCACTC ACCCTCCCAT 
CCTTCTGCTG TTCACCCAGG GTCCCCACCA TCCAGCAATC TTCATGTACC CCTTAGGTCA 
ACTTGGAATT CTGGTCCTGT TCTGGGGTCC CGAACACCTG GTCCACGAAG AATAGACATG 
CCCCCAGATG ATGACTGGAG ACAGAGCAGT TACCCCTCCC AGGACAGACA CAGGAGCCCA 
GGAGAGGAAA GAATTATGTT TGCTCTATCA AATGCTGCTG GAAGAGAGCA GAACAGGGTG 
AGATTTCTAC AGCACAGCAG GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_006519703.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519766.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X1, mRNA.

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CloneID	OMu50452
Clone ID Related Accession (Same CDS sequence)	XM_006519703.3 , XM_011245225.2 , XM_011245225.3 , XM_030248095.1 , XM_006519703.4
Accession Version	XM_006519703.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519766.1
CDS	1000..5937
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_006519703.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519703.3

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_011245225.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243527.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X2, mRNA.

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CloneID	OMu50452
Clone ID Related Accession (Same CDS sequence)	XM_006519703.3 , XM_011245225.2 , XM_011245225.3 , XM_030248095.1 , XM_006519703.4
Accession Version	XM_011245225.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243527.1
CDS	6161..11098
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_011245225.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245225.2

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGATTCATCA 
AACGTGAGGA AAAAGACTTT GGAAACCGGG CACCATTGTT CCAGCTCCTC TTCCCTCCCT 
GTCATCCATG ATCCTCCTGT ATTTCTCCTT GATCCTAAAC TCTACCCTCC CCAACCACAG 
TTCCTGTCCC CAGATGTCCT GATGCCCAGC ATGGCAGGGG AGCCCTATAG ACCCCCAGGA 
ACTTCAAGGA GTGTCCACCA GTTTCTGGCT ATGTGTGACA GGGATGAAAC TCCCCAAGGG 
GTCAAGTACA CAGGAAGGAC TTTGAACTAC CGAAGTCTAC CTCATCGTTC CAGAACTGAT 
GCTTCCTGGG GACCAGGGTC AGAGACCAAC CAGCACATTG GGGCCAGAGT CCTAACTATG 
CCAGCATGCA AGCCTCAGCT AACCTACACT GCCACACTAC CAGAAAGACA CCAGGGCCTT 
CAGGTTCCAC ATGCTCAGTC CTGGGGGAAT CTTTTCCACT CACCCTCCCA TCCTTCTGCT 
GTTCACCCAG GGTCCCCACC ATCCAGCAAT CTTCATGTAC CCCTTAGGTC AACTTGGAAT 
TCTGGTCCTG TTCTGGGGTC CCGAACACCT GGTCCACGAA GAATAGACAT GCCCCCAGAT 
GATGACTGGA GACAGAGCAG TTACCCCTCC CAGGACAGAC ACAGGAGCCC AGGAGAGGAA 
AGAATTATGT TTGCTCTATC AAATGCTGCT GGAAGAGAGC AGAACAGGGT GAGATTTCTA 
CAGCACAGCA GGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_011245226.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243528.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X4, mRNA.

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CloneID	OMu71757
Clone ID Related Accession (Same CDS sequence)	XM_011245226.3 , XM_011245226.2
Accession Version	XM_011245226.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTC ACTTCCATAT TGCTGATGAA ACCAAAGAGG ATATATGTAC TGCTCAACAC 
TGCATATCCC ACCAGAAATT TGCAATGACA TTGTTTGAGC AGTGTGTGTG CAGCAGCTGT 
GGCGCCACGT CCGACCCGCT GCCCTTTATC CAGATGGTGC ACTACATCTC CACCACTGCC 
CTCTGTAACC AGGCTATTTG TATGCTGGAA AAGCGAGAGA AACCCTCACC AAGTATGTTT 
GGTGAGCTGC TTCAGAATGC CAGCACCATG GGGGACCTGC GGAACTGTCC GAGCAACTGT 
GGGGAGAGAA TCCGGATCCG CCGTGTCTTG ATGAATGCAC CACAGATTAT CACGATTGGT 
CTGGTTTGGG ACTCAGAGCA TTCAGACTTG GCAGAGGACG TCATCCACAG CCTGGGCACC 
TGCCTTAAGC TGGGTGATCT GTTTTTCAGA GTGACAGATG ACCGGGCCAA GCAGTCTGAA 
CTATACTTAG TAGGAATGAT CTGTTACTAT GGCAAGCATT ATTCTACATT CTTTTTTCAA 
ACAAAGATCC GCAAATGGAT GTATTTTGAT GATGCTCATG TCAAGGAGAT TGGTCCGAAG 
TGGAAGGATG TGGTCACCAA ATGCATTAAA GGGCACTATC AACCCTTGCT GCTGCTCTAT 
GCAGACCCCC AGGGTACTCC AGTGTCTGCC CAGGACCTGC CTCCCCACGC TCAGTTCCTG 
CCATACACCA AGACATGCTA CGATAGTGAA GACTCAGGGA GGGAGCCTTC CATCTCAAGT 
GACACTCGAA CAGATTCCTC AACGGAGAGC TATCCCTACA AACATTTTCA CCTTGAGTCT 
GTGGTCAGTC ACTTCTCTTC AGATTCTCAG GGGACAGTCA TCTATAATGT GGAGAATGAT 
TCCGTGTCTC AGAGCAGTCG GGACACAGGA CACCTGACTG ATAGTGAATG TAATCAGAAA 
CACACATCGA AGAAAGGGTC CCTGGTGGAG CGCAGGAGGA GCAGTGGCCG TGTTCGGAGA 
AAAGGCGATG AGCCACAGGC CTCAGGGTAC CACAGCGAAG GAGAAACACT GAAAGAGAAG 
CAGGCTCCTA GGAATGCCTC TAAATCCAGC AGCAGCAGCA GGCTAAAGGA TTTCAAAGAG 
ACAGTCAGCA ACATGATCCA CAGCAGGCCT TCCCTGGCTT CTCAGACCAG TGCAGGATCT 
CCCTGTGTAG GTAGGGCAGG AGACCAGCTA GACAAGATAC CACCAAGGAA CTTCCCTTTA 
CAGGCTCGGG GCTGGGAAAC GGAGAGTACC AGTAGTGAGG CAAAATCCAG TTCTTCCAGC 
AAGTACCGCC CAACATGGAG ACCTAAACGA GAATCTCTAA ATATTGACAG CATTTTTAGT 
AAGGACAGAA GGAAGCATTG TGGCTACACC CAGCTTAGGA CCTTTCCTGA AGATGCAGCT 
AAAGAATTTA CACCGGATGA AGTAAGCAAG CCAACTGCTA ATGACATTAA AGATGGCGGA 
TCAAGGTCAC AGCATAAACT CTGGGGAACA GCTCGGCCAG GCTCTCATCT TTTAGAGCAG 
CATCCTCGCC TAATCCAGCG GATGGAATCT GGCTATGAGA GCAGTGAGAG GAACAGCAGC 
AGCCCTGTCA GTTTGGATGC TGCCCCGCCT GACAGCGTGA ATGTCTACAG GGATCAAAGC 
ACAAAGAGGC CTGTTGGGTT TGTCCCTTCT TGGCGTCATA TTCCAAAGTC TCACAGCAGT 
AGCATCCTGG AGGTAGACTG TACAGCACCC ATGACCAGCT GGACAAAGAC TCAACCTCTC 
TCAGATGGGG AGGTCACCTC TAGAAGTGAA TTGGATGAAC TACAGGAAGA AGTGGTCAGG 
CGAGCCCAGG AACAGGAGCT GCGGAAGAAA CGGGAGAAGG AATTAGAGGC TGCTAAAGGC 
TTTAACCCTC ATCCCAGCCG CTACATGGAT TTGGATGAAC TGCAGAATCA GGGGAGGAGT 
GACGGCTTTG AAAGGTCCTT GCAAGAGGCA AATTCAATAT TTGAAGAGTC GCTGCATCTG 
GAACAAAAGG GAGACTGTGC TGCAGCTCTG GCTCTGTGTA ACGAAGCTAT CTCTAAACTA 
AGACTTACCC TGCATGATGC CAGCTCTAGC ACGCACAGCA GAGCCCTAGT CGATAAGAAG 
TTGCAAATCA GTATTCGAAA AGCACGAAGC CTTCAGGATC GCATGCAGCA ACAAGCATCA 
TCGCAGCAGC CGGTGCAGCC CTCAGCCTCT CTCCCATCAC AGGGGGGCGC CCTCCCTCAG 
CCGACAAGTG AACAGCCTAT CACGCTCCAA GTATTGTTAA ACCAGGAGGC ACAGCTGGAA 
CCTTGCAAGG ATACAGAGCT TGGGGCCACT TCCTCTTTCT TCCACTCACC TGCTTCCTGC 
CCTGAGTTAC ACTCATCACT CATCCCAGAG TCACCTGTTT CATCTGTTTC ACAGCACAGT 
CCCCCTGGGA CATCTTCCTT GAAGCTTCTG ACGTCTTTTG AGGTGGACAG CGTCAATCGC 
TCTGCATTCC ACAGGCAGGG TTTACCTAAA GCAACAGGTA GAACTGAGAT GAACTCTCAA 
CATGAATGCC TGCCATTAGA TGCCCTTGAA GATAGGCTCC AAGGCCACAG GGAGGACAAC 
AGCTGTTGCA GCAAATTCCC ACCACGAGAA GGAAGAGACA TAGCTCAAGA TCAGCTGTTT 
GAAGGAAAAA AGAATCCTGC TGATATATCC ATGGGGATGC CTTGGTCATA TTCAACAGGA 
GAAGCCACTT CTGAGAGAGT TATATATAGC CTAAATAGTC CTTCAAGTTC ATCAGCTCAG 
CCTAGTATTC CCCCTTACAG TTCCTGCCAC CCCATCACGT CTGCTGCTTC TTCACCTGTG 
CTTCATGCTG CTGACCCCAT GCAGAAACTT AACCAACACG TTCAAGCCCA AAGTCTCCAG 
ACTTCATTGA CTTCAAAAGT GGTCAGAAGC AGTGAGGAGC CCTATAGGCT AGAGTTCCCC 
AGTACAAAAG GACTTGTCCG CTCATTGGCA GAGCAATTCC AGAAGATGCA GAATACTTCC 
ACGAGAGATG TTATAGGTTC CCAGGATAGA AGTTTGCCAA ATGGTGTCAG GAAGAGCTCT 
TCTCCTTCTG ACTTTATGCC TCCATTATCC CAGGGTCCAG GAAGAGAGCA CTGTCGCTGG 
GTAAAACAAC CCCGTTCCCC CGATGGCAGG GAAAGACCGC CCTGCTGGGA AGACCCTGCA 
GCTCATCCTC CTCTTTCCAT GGGCTCGGGG CTTCCTGATG GTGAAACCTC GAGGACAGCA 
CAGCCCAGGC TGGCTGAGCC AGATATGTAC CAAGGAAAGC TACCCCAAGT GACAGATATT 
AGGTCCAAGG AGCTGGGTAG TAGTGTCAAT TTGGGGACTT CTTTGCCTTT GGATTCTTGG 
GTAAATGTCA CAAGGCTCTG TGATTCTCAG GTTAAACCCA GTGCCCCTGG GCCCGGAGAC 
AAGTCCTCCT CCCATGATTC CCACCCACGT GCAACCTGCC TAGAAAGAAG TCCTATCCTT 
TTGCATCCAC ACTGGGACCA AGACACAGAG CAGGAGACCT CTGAACTGGA GTCTCTCTAC 
CAAGCCAGCC TTCAGGCCTC TTCTCACACT GCCTATTCTG ATTGGAGATC ACAGGATGTG 
GCCTGGCAGC CACTTAGCCT AGCAGGCTCT GCAGATGGCA TGGGAAGGAG ACTGCACTCA 
GCCCCTGGGC TTGATCTCTC AAAGCCTCTG ACAGCAGAGA TGGAGCATGT CCTTTATGAA 
CCGAGCACAG TGCCTGTCTC TCAGGATTCA TCAAACGTGA GGAAAAAGAC TTTGGAAACC 
GGGCACCATT GTTCCAGCTC CTCTTCCCTC CCTGTCATCC ATGATCCTCC TGTATTTCTC 
CTTGATCCTA AACTCTACCC TCCCCAACCA CAGTTCCTGT CCCCAGATGT CCTGATGCCC 
AGCATGGCAG GGGAGCCCTA TAGACCCCCA GGAACTTCAA GGAGTGTCCA CCAGTTTCTG 
GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT ACACAGGAAG GACTTTGAAC 
TACCGAAGTC TACCTCATCG TTCCAGAACT GATGCTTCCT GGGGACCAGG GTCAGAGACC 
AACCAGCACA TTGGGGCCAG AGTCCTAACT ATGCCAGCAT GCAAGCCTCA GCTAACCTAC 
ACTGCCACAC TACCAGAAAG ACACCAGGGC CTTCAGGTTC CACATGCTCA GTCCTGGGGG 
AATCTTTTCC ACTCACCCTC CCATCCTTCT GCTGTTCACC CAGGGTCCCC ACCATCCAGC 
AATCTTCATG TACCCCTTAG GTCAACTTGG AATTCTGGTC CTGTTCTGGG GTCCCGAACA 
CCTGGTCCAC GAAGAATAGA CATGCCCCCA GATGATGACT GGAGACAGAG CAGTTACCCC 
TCCCAGGACA GACACAGGAG CCCAGGAGAG GAAAGAATTA TGTTTGCTCT ATCAAATGCT 
GCTGGAAGAG AGCAGAACAG GGTGAGATTT CTACAGCACA GCAGGTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243528.1
CDS	37..4587
Translation	MRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQDSSNVRKKTLETGHHCSSSSSLPVIHDPPVFLLDPKLYPPQPQFLSPDVLMPSMAGEPYRPPGTSRSVHQFLAMCDRDETPQGVKYTGRTLNYRSLPHRSRTDASWGPGSETNQHIGARVLTMPACKPQLTYTATLPERHQGLQVPHAQSWGNLFHSPSHPSAVHPGSPPSSNLHVPLRSTWNSGPVLGSRTPGPRRIDMPPDDDWRQSSYPSQDRHRSPGEERIMFALSNAAGREQNRVRFLQHSRW

Target ORF information:

RefSeq Version	XM_011245226.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245226.2

ORF Insert Sequence:

ATGAGAATTC ACTTCCATAT TGCTGATGAA ACCAAAGAGG ATATATGTAC TGCTCAACAC 
TGCATATCCC ACCAGAAATT TGCAATGACA TTGTTTGAGC AGTGTGTGTG CAGCAGCTGT 
GGCGCCACGT CCGACCCGCT GCCCTTTATC CAGATGGTGC ACTACATCTC CACCACTGCC 
CTCTGTAACC AGGCTATTTG TATGCTGGAA AAGCGAGAGA AACCCTCACC AAGTATGTTT 
GGTGAGCTGC TTCAGAATGC CAGCACCATG GGGGACCTGC GGAACTGTCC GAGCAACTGT 
GGGGAGAGAA TCCGGATCCG CCGTGTCTTG ATGAATGCAC CACAGATTAT CACGATTGGT 
CTGGTTTGGG ACTCAGAGCA TTCAGACTTG GCAGAGGACG TCATCCACAG CCTGGGCACC 
TGCCTTAAGC TGGGTGATCT GTTTTTCAGA GTGACAGATG ACCGGGCCAA GCAGTCTGAA 
CTATACTTAG TAGGAATGAT CTGTTACTAT GGCAAGCATT ATTCTACATT CTTTTTTCAA 
ACAAAGATCC GCAAATGGAT GTATTTTGAT GATGCTCATG TCAAGGAGAT TGGTCCGAAG 
TGGAAGGATG TGGTCACCAA ATGCATTAAA GGGCACTATC AACCCTTGCT GCTGCTCTAT 
GCAGACCCCC AGGGTACTCC AGTGTCTGCC CAGGACCTGC CTCCCCACGC TCAGTTCCTG 
CCATACACCA AGACATGCTA CGATAGTGAA GACTCAGGGA GGGAGCCTTC CATCTCAAGT 
GACACTCGAA CAGATTCCTC AACGGAGAGC TATCCCTACA AACATTTTCA CCTTGAGTCT 
GTGGTCAGTC ACTTCTCTTC AGATTCTCAG GGGACAGTCA TCTATAATGT GGAGAATGAT 
TCCGTGTCTC AGAGCAGTCG GGACACAGGA CACCTGACTG ATAGTGAATG TAATCAGAAA 
CACACATCGA AGAAAGGGTC CCTGGTGGAG CGCAGGAGGA GCAGTGGCCG TGTTCGGAGA 
AAAGGCGATG AGCCACAGGC CTCAGGGTAC CACAGCGAAG GAGAAACACT GAAAGAGAAG 
CAGGCTCCTA GGAATGCCTC TAAATCCAGC AGCAGCAGCA GGCTAAAGGA TTTCAAAGAG 
ACAGTCAGCA ACATGATCCA CAGCAGGCCT TCCCTGGCTT CTCAGACCAG TGCAGGATCT 
CCCTGTGTAG GTAGGGCAGG AGACCAGCTA GACAAGATAC CACCAAGGAA CTTCCCTTTA 
CAGGCTCGGG GCTGGGAAAC GGAGAGTACC AGTAGTGAGG CAAAATCCAG TTCTTCCAGC 
AAGTACCGCC CAACATGGAG ACCTAAACGA GAATCTCTAA ATATTGACAG CATTTTTAGT 
AAGGACAGAA GGAAGCATTG TGGCTACACC CAGCTTAGGA CCTTTCCTGA AGATGCAGCT 
AAAGAATTTA CACCGGATGA AGTAAGCAAG CCAACTGCTA ATGACATTAA AGATGGCGGA 
TCAAGGTCAC AGCATAAACT CTGGGGAACA GCTCGGCCAG GCTCTCATCT TTTAGAGCAG 
CATCCTCGCC TAATCCAGCG GATGGAATCT GGCTATGAGA GCAGTGAGAG GAACAGCAGC 
AGCCCTGTCA GTTTGGATGC TGCCCCGCCT GACAGCGTGA ATGTCTACAG GGATCAAAGC 
ACAAAGAGGC CTGTTGGGTT TGTCCCTTCT TGGCGTCATA TTCCAAAGTC TCACAGCAGT 
AGCATCCTGG AGGTAGACTG TACAGCACCC ATGACCAGCT GGACAAAGAC TCAACCTCTC 
TCAGATGGGG AGGTCACCTC TAGAAGTGAA TTGGATGAAC TACAGGAAGA AGTGGTCAGG 
CGAGCCCAGG AACAGGAGCT GCGGAAGAAA CGGGAGAAGG AATTAGAGGC TGCTAAAGGC 
TTTAACCCTC ATCCCAGCCG CTACATGGAT TTGGATGAAC TGCAGAATCA GGGGAGGAGT 
GACGGCTTTG AAAGGTCCTT GCAAGAGGCA AATTCAATAT TTGAAGAGTC GCTGCATCTG 
GAACAAAAGG GAGACTGTGC TGCAGCTCTG GCTCTGTGTA ACGAAGCTAT CTCTAAACTA 
AGACTTACCC TGCATGATGC CAGCTCTAGC ACGCACAGCA GAGCCCTAGT CGATAAGAAG 
TTGCAAATCA GTATTCGAAA AGCACGAAGC CTTCAGGATC GCATGCAGCA ACAAGCATCA 
TCGCAGCAGC CGGTGCAGCC CTCAGCCTCT CTCCCATCAC AGGGGGGCGC CCTCCCTCAG 
CCGACAAGTG AACAGCCTAT CACGCTCCAA GTATTGTTAA ACCAGGAGGC ACAGCTGGAA 
CCTTGCAAGG ATACAGAGCT TGGGGCCACT TCCTCTTTCT TCCACTCACC TGCTTCCTGC 
CCTGAGTTAC ACTCATCACT CATCCCAGAG TCACCTGTTT CATCTGTTTC ACAGCACAGT 
CCCCCTGGGA CATCTTCCTT GAAGCTTCTG ACGTCTTTTG AGGTGGACAG CGTCAATCGC 
TCTGCATTCC ACAGGCAGGG TTTACCTAAA GCAACAGGTA GAACTGAGAT GAACTCTCAA 
CATGAATGCC TGCCATTAGA TGCCCTTGAA GATAGGCTCC AAGGCCACAG GGAGGACAAC 
AGCTGTTGCA GCAAATTCCC ACCACGAGAA GGAAGAGACA TAGCTCAAGA TCAGCTGTTT 
GAAGGAAAAA AGAATCCTGC TGATATATCC ATGGGGATGC CTTGGTCATA TTCAACAGGA 
GAAGCCACTT CTGAGAGAGT TATATATAGC CTAAATAGTC CTTCAAGTTC ATCAGCTCAG 
CCTAGTATTC CCCCTTACAG TTCCTGCCAC CCCATCACGT CTGCTGCTTC TTCACCTGTG 
CTTCATGCTG CTGACCCCAT GCAGAAACTT AACCAACACG TTCAAGCCCA AAGTCTCCAG 
ACTTCATTGA CTTCAAAAGT GGTCAGAAGC AGTGAGGAGC CCTATAGGCT AGAGTTCCCC 
AGTACAAAAG GACTTGTCCG CTCATTGGCA GAGCAATTCC AGAAGATGCA GAATACTTCC 
ACGAGAGATG TTATAGGTTC CCAGGATAGA AGTTTGCCAA ATGGTGTCAG GAAGAGCTCT 
TCTCCTTCTG ACTTTATGCC TCCATTATCC CAGGGTCCAG GAAGAGAGCA CTGTCGCTGG 
GTAAAACAAC CCCGTTCCCC CGATGGCAGG GAAAGACCGC CCTGCTGGGA AGACCCTGCA 
GCTCATCCTC CTCTTTCCAT GGGCTCGGGG CTTCCTGATG GTGAAACCTC GAGGACAGCA 
CAGCCCAGGC TGGCTGAGCC AGATATGTAC CAAGGAAAGC TACCCCAAGT GACAGATATT 
AGGTCCAAGG AGCTGGGTAG TAGTGTCAAT TTGGGGACTT CTTTGCCTTT GGATTCTTGG 
GTAAATGTCA CAAGGCTCTG TGATTCTCAG GTTAAACCCA GTGCCCCTGG GCCCGGAGAC 
AAGTCCTCCT CCCATGATTC CCACCCACGT GCAACCTGCC TAGAAAGAAG TCCTATCCTT 
TTGCATCCAC ACTGGGACCA AGACACAGAG CAGGAGACCT CTGAACTGGA GTCTCTCTAC 
CAAGCCAGCC TTCAGGCCTC TTCTCACACT GCCTATTCTG ATTGGAGATC ACAGGATGTG 
GCCTGGCAGC CACTTAGCCT AGCAGGCTCT GCAGATGGCA TGGGAAGGAG ACTGCACTCA 
GCCCCTGGGC TTGATCTCTC AAAGCCTCTG ACAGCAGAGA TGGAGCATGT CCTTTATGAA 
CCGAGCACAG TGCCTGTCTC TCAGGATTCA TCAAACGTGA GGAAAAAGAC TTTGGAAACC 
GGGCACCATT GTTCCAGCTC CTCTTCCCTC CCTGTCATCC ATGATCCTCC TGTATTTCTC 
CTTGATCCTA AACTCTACCC TCCCCAACCA CAGTTCCTGT CCCCAGATGT CCTGATGCCC 
AGCATGGCAG GGGAGCCCTA TAGACCCCCA GGAACTTCAA GGAGTGTCCA CCAGTTTCTG 
GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT ACACAGGAAG GACTTTGAAC 
TACCGAAGTC TACCTCATCG TTCCAGAACT GATGCTTCCT GGGGACCAGG GTCAGAGACC 
AACCAGCACA TTGGGGCCAG AGTCCTAACT ATGCCAGCAT GCAAGCCTCA GCTAACCTAC 
ACTGCCACAC TACCAGAAAG ACACCAGGGC CTTCAGGTTC CACATGCTCA GTCCTGGGGG 
AATCTTTTCC ACTCACCCTC CCATCCTTCT GCTGTTCACC CAGGGTCCCC ACCATCCAGC 
AATCTTCATG TACCCCTTAG GTCAACTTGG AATTCTGGTC CTGTTCTGGG GTCCCGAACA 
CCTGGTCCAC GAAGAATAGA CATGCCCCCA GATGATGACT GGAGACAGAG CAGTTACCCC 
TCCCAGGACA GACACAGGAG CCCAGGAGAG GAAAGAATTA TGTTTGCTCT ATCAAATGCT 
GCTGGAAGAG AGCAGAACAG GGTGAGATTT CTACAGCACA GCAGGTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp54 ( XM_011245227.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243529.1 Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X5, mRNA.

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CloneID	OMu71758
Clone ID Related Accession (Same CDS sequence)	XM_011245227.2 , XM_011245227.3
Accession Version	XM_011245227.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGAACTTCAA 
GGAGTGTCCA CCAGTTTCTG GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT 
ACACAGGAAG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243529.1
CDS	1000..5277
Translation	MSWKRNYFSGSRGSVQGMFAPRSSMSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCSSCGATSDPLPFIQMVHYISTTALCNQAICMLEKREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSEHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSAQDLPPHAQFLPYTKTCYDSEDSGREPSISSDTRTDSSTESYPYKHFHLESVVSHFSSDSQGTVIYNVENDSVSQSSRDTGHLTDSECNQKHTSKKGSLVERRRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKSSSSSRLKDFKETVSNMIHSRPSLASQTSAGSPCVGRAGDQLDKIPPRNFPLQARGWETESTSSEAKSSSSSKYRPTWRPKRESLNIDSIFSKDRRKHCGYTQLRTFPEDAAKEFTPDEVSKPTANDIKDGGSRSQHKLWGTARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAAPPDSVNVYRDQSTKRPVGFVPSWRHIPKSHSSSILEVDCTAPMTSWTKTQPLSDGEVTSRSELDELQEEVVRRAQEQELRKKREKELEAAKGFNPHPSRYMDLDELQNQGRSDGFERSLQEANSIFEESLHLEQKGDCAAALALCNEAISKLRLTLHDASSSTHSRALVDKKLQISIRKARSLQDRMQQQASSQQPVQPSASLPSQGGALPQPTSEQPITLQVLLNQEAQLEPCKDTELGATSSFFHSPASCPELHSSLIPESPVSSVSQHSPPGTSSLKLLTSFEVDSVNRSAFHRQGLPKATGRTEMNSQHECLPLDALEDRLQGHREDNSCCSKFPPREGRDIAQDQLFEGKKNPADISMGMPWSYSTGEATSERVIYSLNSPSSSSAQPSIPPYSSCHPITSAASSPVLHAADPMQKLNQHVQAQSLQTSLTSKVVRSSEEPYRLEFPSTKGLVRSLAEQFQKMQNTSTRDVIGSQDRSLPNGVRKSSSPSDFMPPLSQGPGREHCRWVKQPRSPDGRERPPCWEDPAAHPPLSMGSGLPDGETSRTAQPRLAEPDMYQGKLPQVTDIRSKELGSSVNLGTSLPLDSWVNVTRLCDSQVKPSAPGPGDKSSSHDSHPRATCLERSPILLHPHWDQDTEQETSELESLYQASLQASSHTAYSDWRSQDVAWQPLSLAGSADGMGRRLHSAPGLDLSKPLTAEMEHVLYEPSTVPVSQELQGVSTSFWLCVTGMKLPKGSSTQEGL

Target ORF information:

RefSeq Version	XM_011245227.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 54 (Usp54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245227.2

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGGA AGCCGAGGTA GTGTTCAAGG GATGTTCGCA 
CCTCGGAGCT CAATGTCCAT TGCCCCCAGC AAAGGCCTCA GCAATGAGCC AGGGCAAAAC 
AGCTGCTTCC TCAACAGTGC CCTGCAGGTT TTGTGGCACT TGGACATCTT CCGCCGTAGC 
TTTCGGCAGC TTACAACTCA CAAGTGCATG GGTGATTCCT GCATCTTCTG TGCTCTCAAG 
GGCATCTTTA ACCAGTTTCA GTGTAGTAGT GAAAAAGTGC TTCCTTCCGA CACTCTCCGC 
AGTGCTCTGG CAAAGACTTT CCAAGATGAA CAACGCTTCC AGCTGGGGAT TATGGATGAT 
GCTGCAGAAT GCTTTGAAAA CCTCCTGATG AGAATTCACT TCCATATTGC TGATGAAACC 
AAAGAGGATA TATGTACTGC TCAACACTGC ATATCCCACC AGAAATTTGC AATGACATTG 
TTTGAGCAGT GTGTGTGCAG CAGCTGTGGC GCCACGTCCG ACCCGCTGCC CTTTATCCAG 
ATGGTGCACT ACATCTCCAC CACTGCCCTC TGTAACCAGG CTATTTGTAT GCTGGAAAAG 
CGAGAGAAAC CCTCACCAAG TATGTTTGGT GAGCTGCTTC AGAATGCCAG CACCATGGGG 
GACCTGCGGA ACTGTCCGAG CAACTGTGGG GAGAGAATCC GGATCCGCCG TGTCTTGATG 
AATGCACCAC AGATTATCAC GATTGGTCTG GTTTGGGACT CAGAGCATTC AGACTTGGCA 
GAGGACGTCA TCCACAGCCT GGGCACCTGC CTTAAGCTGG GTGATCTGTT TTTCAGAGTG 
ACAGATGACC GGGCCAAGCA GTCTGAACTA TACTTAGTAG GAATGATCTG TTACTATGGC 
AAGCATTATT CTACATTCTT TTTTCAAACA AAGATCCGCA AATGGATGTA TTTTGATGAT 
GCTCATGTCA AGGAGATTGG TCCGAAGTGG AAGGATGTGG TCACCAAATG CATTAAAGGG 
CACTATCAAC CCTTGCTGCT GCTCTATGCA GACCCCCAGG GTACTCCAGT GTCTGCCCAG 
GACCTGCCTC CCCACGCTCA GTTCCTGCCA TACACCAAGA CATGCTACGA TAGTGAAGAC 
TCAGGGAGGG AGCCTTCCAT CTCAAGTGAC ACTCGAACAG ATTCCTCAAC GGAGAGCTAT 
CCCTACAAAC ATTTTCACCT TGAGTCTGTG GTCAGTCACT TCTCTTCAGA TTCTCAGGGG 
ACAGTCATCT ATAATGTGGA GAATGATTCC GTGTCTCAGA GCAGTCGGGA CACAGGACAC 
CTGACTGATA GTGAATGTAA TCAGAAACAC ACATCGAAGA AAGGGTCCCT GGTGGAGCGC 
AGGAGGAGCA GTGGCCGTGT TCGGAGAAAA GGCGATGAGC CACAGGCCTC AGGGTACCAC 
AGCGAAGGAG AAACACTGAA AGAGAAGCAG GCTCCTAGGA ATGCCTCTAA ATCCAGCAGC 
AGCAGCAGGC TAAAGGATTT CAAAGAGACA GTCAGCAACA TGATCCACAG CAGGCCTTCC 
CTGGCTTCTC AGACCAGTGC AGGATCTCCC TGTGTAGGTA GGGCAGGAGA CCAGCTAGAC 
AAGATACCAC CAAGGAACTT CCCTTTACAG GCTCGGGGCT GGGAAACGGA GAGTACCAGT 
AGTGAGGCAA AATCCAGTTC TTCCAGCAAG TACCGCCCAA CATGGAGACC TAAACGAGAA 
TCTCTAAATA TTGACAGCAT TTTTAGTAAG GACAGAAGGA AGCATTGTGG CTACACCCAG 
CTTAGGACCT TTCCTGAAGA TGCAGCTAAA GAATTTACAC CGGATGAAGT AAGCAAGCCA 
ACTGCTAATG ACATTAAAGA TGGCGGATCA AGGTCACAGC ATAAACTCTG GGGAACAGCT 
CGGCCAGGCT CTCATCTTTT AGAGCAGCAT CCTCGCCTAA TCCAGCGGAT GGAATCTGGC 
TATGAGAGCA GTGAGAGGAA CAGCAGCAGC CCTGTCAGTT TGGATGCTGC CCCGCCTGAC 
AGCGTGAATG TCTACAGGGA TCAAAGCACA AAGAGGCCTG TTGGGTTTGT CCCTTCTTGG 
CGTCATATTC CAAAGTCTCA CAGCAGTAGC ATCCTGGAGG TAGACTGTAC AGCACCCATG 
ACCAGCTGGA CAAAGACTCA ACCTCTCTCA GATGGGGAGG TCACCTCTAG AAGTGAATTG 
GATGAACTAC AGGAAGAAGT GGTCAGGCGA GCCCAGGAAC AGGAGCTGCG GAAGAAACGG 
GAGAAGGAAT TAGAGGCTGC TAAAGGCTTT AACCCTCATC CCAGCCGCTA CATGGATTTG 
GATGAACTGC AGAATCAGGG GAGGAGTGAC GGCTTTGAAA GGTCCTTGCA AGAGGCAAAT 
TCAATATTTG AAGAGTCGCT GCATCTGGAA CAAAAGGGAG ACTGTGCTGC AGCTCTGGCT 
CTGTGTAACG AAGCTATCTC TAAACTAAGA CTTACCCTGC ATGATGCCAG CTCTAGCACG 
CACAGCAGAG CCCTAGTCGA TAAGAAGTTG CAAATCAGTA TTCGAAAAGC ACGAAGCCTT 
CAGGATCGCA TGCAGCAACA AGCATCATCG CAGCAGCCGG TGCAGCCCTC AGCCTCTCTC 
CCATCACAGG GGGGCGCCCT CCCTCAGCCG ACAAGTGAAC AGCCTATCAC GCTCCAAGTA 
TTGTTAAACC AGGAGGCACA GCTGGAACCT TGCAAGGATA CAGAGCTTGG GGCCACTTCC 
TCTTTCTTCC ACTCACCTGC TTCCTGCCCT GAGTTACACT CATCACTCAT CCCAGAGTCA 
CCTGTTTCAT CTGTTTCACA GCACAGTCCC CCTGGGACAT CTTCCTTGAA GCTTCTGACG 
TCTTTTGAGG TGGACAGCGT CAATCGCTCT GCATTCCACA GGCAGGGTTT ACCTAAAGCA 
ACAGGTAGAA CTGAGATGAA CTCTCAACAT GAATGCCTGC CATTAGATGC CCTTGAAGAT 
AGGCTCCAAG GCCACAGGGA GGACAACAGC TGTTGCAGCA AATTCCCACC ACGAGAAGGA 
AGAGACATAG CTCAAGATCA GCTGTTTGAA GGAAAAAAGA ATCCTGCTGA TATATCCATG 
GGGATGCCTT GGTCATATTC AACAGGAGAA GCCACTTCTG AGAGAGTTAT ATATAGCCTA 
AATAGTCCTT CAAGTTCATC AGCTCAGCCT AGTATTCCCC CTTACAGTTC CTGCCACCCC 
ATCACGTCTG CTGCTTCTTC ACCTGTGCTT CATGCTGCTG ACCCCATGCA GAAACTTAAC 
CAACACGTTC AAGCCCAAAG TCTCCAGACT TCATTGACTT CAAAAGTGGT CAGAAGCAGT 
GAGGAGCCCT ATAGGCTAGA GTTCCCCAGT ACAAAAGGAC TTGTCCGCTC ATTGGCAGAG 
CAATTCCAGA AGATGCAGAA TACTTCCACG AGAGATGTTA TAGGTTCCCA GGATAGAAGT 
TTGCCAAATG GTGTCAGGAA GAGCTCTTCT CCTTCTGACT TTATGCCTCC ATTATCCCAG 
GGTCCAGGAA GAGAGCACTG TCGCTGGGTA AAACAACCCC GTTCCCCCGA TGGCAGGGAA 
AGACCGCCCT GCTGGGAAGA CCCTGCAGCT CATCCTCCTC TTTCCATGGG CTCGGGGCTT 
CCTGATGGTG AAACCTCGAG GACAGCACAG CCCAGGCTGG CTGAGCCAGA TATGTACCAA 
GGAAAGCTAC CCCAAGTGAC AGATATTAGG TCCAAGGAGC TGGGTAGTAG TGTCAATTTG 
GGGACTTCTT TGCCTTTGGA TTCTTGGGTA AATGTCACAA GGCTCTGTGA TTCTCAGGTT 
AAACCCAGTG CCCCTGGGCC CGGAGACAAG TCCTCCTCCC ATGATTCCCA CCCACGTGCA 
ACCTGCCTAG AAAGAAGTCC TATCCTTTTG CATCCACACT GGGACCAAGA CACAGAGCAG 
GAGACCTCTG AACTGGAGTC TCTCTACCAA GCCAGCCTTC AGGCCTCTTC TCACACTGCC 
TATTCTGATT GGAGATCACA GGATGTGGCC TGGCAGCCAC TTAGCCTAGC AGGCTCTGCA 
GATGGCATGG GAAGGAGACT GCACTCAGCC CCTGGGCTTG ATCTCTCAAA GCCTCTGACA 
GCAGAGATGG AGCATGTCCT TTATGAACCG AGCACAGTGC CTGTCTCTCA GGAACTTCAA 
GGAGTGTCCA CCAGTTTCTG GCTATGTGTG ACAGGGATGA AACTCCCCAA GGGGTCAAGT 
ACACAGGAAG GACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Usp54
Entrez Gene ID	78787
Full Name	ubiquitin specific peptidase 54
Synonyms	4930429G18Rik,AI115571,C030002J06Rik
General protein information	Preferred Names ubiquitin specific peptidase 54 Names inactive ubiquitin carboxyl-terminal hydrolase 54 inactive ubiquitin-specific peptidase 54 ubiquitin specific protease 54
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 A3

Homo sapiens (human)	USP54	NP_689799.3
Mus musculus (house mouse)	Usp54	NP_084456.2
Canis lupus familiaris (dog)	USP54	XP_005619078.1
Bos taurus (cattle)	USP54	XP_005226561.1
Gallus gallus (chicken)	USP54	XP_004942143.1
Danio rerio (zebrafish)	usp54a	NP_001188495.1
	LOC706859	XP_002808545.1
Rattus norvegicus (Norway rat)	Usp54	NP_001008863.2
Pan troglodytes (chimpanzee)	USP54	XP_003312678.1
Danio rerio (zebrafish)	LOC100535699	XP_003199659.2
Xenopus tropicalis (tropical clawed frog)	usp54	XP_002935811.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Usp54 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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