Spinkl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spinkl
Entrez Gene ID	77424
Full Name	serine protease inhibitor, Kazal type-like
Synonyms	9530002K18Rik
General protein information	Preferred Names serine protease inhibitor, Kazal type-like Names serine protease inhibitor kazal-like protein, minor form SPINKL, minor form
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526352.4	XP_006526415.1	serine protease inhibitor kazal-like protein, minor form isoform X1
NM_183123.2	NP_898946.1	serine protease inhibitor kazal-like protein, minor form precursor
XM_006526352.3	XP_006526415.1	serine protease inhibitor kazal-like protein, minor form isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Spink11	NP_001041682.2
Mus musculus (house mouse)	Spinkl	NP_898946.1

Gene Ontology
Bibliography

Related articles in PubMed

Mechanisms underlying the inhibition of murine sperm capacitation by the seminal protein, SPINKL.
Tseng HC, Lee RK, Hwu YM, Lu CH, Lin MH, Li SH
Journal of cellular biochemistry114(4)888-98(2013 Apr)

SPINKL, a Kazal-type serine protease inhibitor-like protein purified from mouse seminal vesicle fluid, is able to inhibit sperm capacitation.
Lin MH, Lee RK, Hwu YM, Lu CH, Chu SL, Chen YJ, Chang WC, Li SH
Reproduction (Cambridge, England)136(5)559-71(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPINKL's binding to ejaculated sperm in the uterus but not in the oviduct suggest that SPINKL serves as a sperm protector in the uterus by preventing premature capacitation.
Title: Mechanisms underlying the inhibition of murine sperm capacitation by the seminal protein, SPINKL.

The following Spinkl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spinkl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08374	NM_183123.2 Latest version!	Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu08374	XM_006526352.4 Latest version!	Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu08374	XM_006526352.3	Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spinkl ( NM_183123.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_898946.1 Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), mRNA.

Price & Availability↑

CloneID	OMu08374
Clone ID Related Accession (Same CDS sequence)	XM_006526352.4 , NM_183123.2 , XM_006526352.3
Accession Version	NM_183123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor kazal-like protein, minor form precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK020535.1. On or before Jul 2, 2009 this sequence version replaced XR_035343.1, XR_035394.1, NM_183123.1. ##Evidence-Data-START## Transcript exon combination :: AK020535.1, BB873722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTCAA CATGGATCAA ATTTCTTTTT ATTCTGACTT TGGTGCTTCT TCCTTATTCT 
GTGTTTTCAG TGAATATCTT TGCAGGTCCA GAGAATGTTA TTAAAGAGCC AAATTGCACC 
ATGTATAAGA GTAAATCTGA GTGCTCCAAT ATCGCTGAGA ACCCAGTTTG TGCAGATGAT 
AGAAATACAT ATTACAATGA GTGTTACTTC TGCATAGAAA AAGTAGTGGA AAAACTTAAA 
TATAGGTATC ATGGTATTTG TATTTATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_898946.1
CDS	55..327
Translation	MSSTWIKFLFILTLVLLPYSVFSVNIFAGPENVIKEPNCTMYKSKSECSNIAENPVCADDRNTYYNECYFCIEKVVEKLKYRYHGICIYK

Target ORF information:

RefSeq Version	NM_183123.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183123.2

ORF Insert Sequence:

ATGTCCTCAA CATGGATCAA ATTTCTTTTT ATTCTGACTT TGGTGCTTCT TCCTTATTCT 
GTGTTTTCAG TGAATATCTT TGCAGGTCCA GAGAATGTTA TTAAAGAGCC AAATTGCACC 
ATGTATAAGA GTAAATCTGA GTGCTCCAAT ATCGCTGAGA ACCCAGTTTG TGCAGATGAT 
AGAAATACAT ATTACAATGA GTGTTACTTC TGCATAGAAA AAGTAGTGGA AAAACTTAAA 
TATAGGTATC ATGGTATTTG TATTTATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spinkl ( XM_006526352.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526415.1 Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu08374
Clone ID Related Accession (Same CDS sequence)	XM_006526352.4 , NM_183123.2 , XM_006526352.3
Accession Version	XM_006526352.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor kazal-like protein, minor form isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCAA CATGGATCAA ATTTCTTTTT ATTCTGACTT TGGTGCTTCT TCCTTATTCT 
GTGTTTTCAG TGAATATCTT TGCAGGTCCA GAGAATGTTA TTAAAGAGCC AAATTGCACC 
ATGTATAAGA GTAAATCTGA GTGCTCCAAT ATCGCTGAGA ACCCAGTTTG TGCAGATGAT 
AGAAATACAT ATTACAATGA GTGTTACTTC TGCATAGAAA AAGTAGTGGA AAAACTTAAA 
TATAGGTATC ATGGTATTTG TATTTATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526415.1
CDS	54..326
Translation	MSSTWIKFLFILTLVLLPYSVFSVNIFAGPENVIKEPNCTMYKSKSECSNIAENPVCADDRNTYYNECYFCIEKVVEKLKYRYHGICIYK

Target ORF information:

RefSeq Version	XM_006526352.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526352.3

ORF Insert Sequence:

ATGTCCTCAA CATGGATCAA ATTTCTTTTT ATTCTGACTT TGGTGCTTCT TCCTTATTCT 
GTGTTTTCAG TGAATATCTT TGCAGGTCCA GAGAATGTTA TTAAAGAGCC AAATTGCACC 
ATGTATAAGA GTAAATCTGA GTGCTCCAAT ATCGCTGAGA ACCCAGTTTG TGCAGATGAT 
AGAAATACAT ATTACAATGA GTGTTACTTC TGCATAGAAA AAGTAGTGGA AAAACTTAAA 
TATAGGTATC ATGGTATTTG TATTTATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spinkl ( XM_006526352.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526415.1 Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu08374
Clone ID Related Accession (Same CDS sequence)	XM_006526352.4 , NM_183123.2 , XM_006526352.3
Accession Version	XM_006526352.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor kazal-like protein, minor form isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526352.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCAA CATGGATCAA ATTTCTTTTT ATTCTGACTT TGGTGCTTCT TCCTTATTCT 
GTGTTTTCAG TGAATATCTT TGCAGGTCCA GAGAATGTTA TTAAAGAGCC AAATTGCACC 
ATGTATAAGA GTAAATCTGA GTGCTCCAAT ATCGCTGAGA ACCCAGTTTG TGCAGATGAT 
AGAAATACAT ATTACAATGA GTGTTACTTC TGCATAGAAA AAGTAGTGGA AAAACTTAAA 
TATAGGTATC ATGGTATTTG TATTTATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526415.1
CDS	54..326
Translation	MSSTWIKFLFILTLVLLPYSVFSVNIFAGPENVIKEPNCTMYKSKSECSNIAENPVCADDRNTYYNECYFCIEKVVEKLKYRYHGICIYK

Target ORF information:

RefSeq Version	XM_006526352.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine protease inhibitor, Kazal type-like (Spinkl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526352.4

ORF Insert Sequence:

ATGTCCTCAA CATGGATCAA ATTTCTTTTT ATTCTGACTT TGGTGCTTCT TCCTTATTCT 
GTGTTTTCAG TGAATATCTT TGCAGGTCCA GAGAATGTTA TTAAAGAGCC AAATTGCACC 
ATGTATAAGA GTAAATCTGA GTGCTCCAAT ATCGCTGAGA ACCCAGTTTG TGCAGATGAT 
AGAAATACAT ATTACAATGA GTGTTACTTC TGCATAGAAA AAGTAGTGGA AAAACTTAAA 
TATAGGTATC ATGGTATTTG TATTTATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanisms underlying the inhibition of murine sperm capacitation by the seminal protein, SPINKL.
Journal of cellular biochemistry114(4)888-98(2013 Apr)
Tseng HC,Lee RK,Hwu YM,Lu CH,Lin MH,Li SH

SPINKL, a Kazal-type serine protease inhibitor-like protein purified from mouse seminal vesicle fluid, is able to inhibit sperm capacitation.
Reproduction (Cambridge, England)136(5)559-71(2008 Nov)
Lin MH,Lee RK,Hwu YM,Lu CH,Chu SL,Chen YJ,Chang WC,Li SH