Rasd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasd2
Entrez Gene ID	75141
Full Name	RASD family, member 2
Synonyms	4930526B11Rik,AU045414,Rhes,TEM-2,TEM2
General protein information	Preferred Names RASD family, member 2 Names GTP-binding protein Rhes ras homolog enriched in striatum
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_029182.1	NP_083458.1	GTP-binding protein Rhes

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RASD2	XP_004937854.1
Homo sapiens (human)	RASD2	NP_055125.2
Bos taurus (cattle)	RASD2	NP_001075063.1
Mus musculus (house mouse)	Rasd2	NP_083458.1
Xenopus tropicalis (tropical clawed frog)	rasd2	NP_001016006.1
Pan troglodytes (chimpanzee)	RASD2	XP_001156301.2
Canis lupus familiaris (dog)	RASD2	XP_538405.3
Rattus norvegicus (Norway rat)	Rasd2	NP_598252.1

Gene Ontology
Bibliography

Related articles in PubMed

An Atoh1-S193A Phospho-Mutant Allele Causes Hearing Deficits and Motor Impairment.
Xie WR, Jen HI, Seymour ML, Yeh SY, Pereira FA, Groves AK, Klisch TJ, Zoghbi HY
The Journal of neuroscience : the official journal of the Society for Neuroscience37(36)8583-8594(2017 09)

Genome-wide Analysis of RAR? Transcriptional Targets in Mouse Striatum Links Retinoic Acid Signaling with Huntington's Disease and Other Neurodegenerative Disorders.
Niewiadomska-Cimicka A, Krzy?osiak A, Ye T, Podle?ny-Drabiniok A, Demb?l? D, Doll? P, Kr??el W
Molecular neurobiology54(5)3859-3878(2017 Jul)

RasGRP1 promotes amphetamine-induced motor behavior through a Rhes interaction network ("Rhesactome") in the striatum.
Shahani N, Swarnkar S, Giovinazzo V, Morgenweck J, Bohn LM, Scharager-Tapia C, Pascal B, Martinez-Acedo P, Khare K, Subramaniam S
Science signaling9(454)ra111(2016 Nov)

Genetic deletion of Rhes or pharmacological blockade of mTORC1 prevent striato-nigral neurons activation in levodopa-induced dyskinesia.
Brugnoli A, Napolitano F, Usiello A, Morari M
Neurobiology of disease85155-163(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

guanine nucleotide exchange factor RasGRP1 inhibited Rhes-mediated control of striatal motor activity in mice. RasGRP1 stabilized Rhes, increasing its synaptic accumulation in the striatum.
Title: RasGRP1 promotes amphetamine-induced motor behavior through a Rhes interaction network ("Rhesactome") in the striatum.

that Rasd2 mutants display deficits in basal prepulse inhibition that, in turn, exacerbate gating disruption under psychotomimetic drug challenge
Title: Rasd2 Modulates Prefronto-Striatal Phenotypes in Humans and 'Schizophrenia-Like Behaviors' in Mice.

Study showed that both genetic deletion of Rhes and pharmacological blockade of mTORC1 significantly attenuate dyskinesia development by reducing the sensitization of striato-nigral medium-sized spiny neurons to levodopa
Title: Genetic deletion of Rhes or pharmacological blockade of mTORC1 prevent striato-nigral neurons activation in levodopa-induced dyskinesia.

ectopic expression of Rhes in the cerebellum of N171-82Q mice, during the asymptomatic period led to an exacerbation of motor deficits, including loss of balance and motor incoordination with ataxia-like features
Title: Ectopic expression of the striatal-enriched GTPase Rhes elicits cerebellar degeneration and an ataxia phenotype in Huntington's disease.

Rhes influences striatal cAMP/PKA-dependent signaling and synaptic plasticity in a gender-sensitive fashion
Title: Rhes influences striatal cAMP/PKA-dependent signaling and synaptic plasticity in a gender-sensitive fashion.

The following Rasd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18222	NM_029182.1 Latest version!	Mus musculus RASD family, member 2 (Rasd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasd2 ( NM_029182.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083458.1 Mus musculus RASD family, member 2 (Rasd2), mRNA.

Price & Availability↑

CloneID	OMu18222
Clone ID Related Accession (Same CDS sequence)	NM_029182.1
Accession Version	NM_029182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	GTP-binding protein Rhes
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC076974.33. On or before Oct 31, 2008 this sequence version replaced XM_905295.3, XM_204287.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036988.1, SRR1660815.24040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATGAAGA CCTTGTCCAG TGGGAACTGC ACACTCAATG TGCCTGCTAA GAACTCCTAC 
CGCATGGTGG TGCTGGGTGC CTCCCGAGTG GGCAAGAGCT CCATTGTCTC CCGCTTCCTC 
AATGGCCGCT TTGAGGACCA GTACACGCCC ACTATCGAGG ACTTTCATCG CAAGGTGTAC 
AACATCCACG GGGACATGTA CCAGCTGGAT ATCCTGGACA CCTCTGGCAA CCACCCATTC 
CCTGCCATGC GCCGGCTCTC CATCCTCACA GGAGATGTCT TCATCCTGGT GTTCAGCCTG 
GATAGCCGGG AGTCCTTTGA TGAGGTCAAG CGCCTCCAGA AACAGATCCT GGAGGTCAAG 
TCCTGCCTGA AGAATAAAAC CAAGGAGGCA GCAGAGCTGC CCATGGTGAT CTGTGGGAAC 
AAGAATGACC ACAGTGAGCT GTGCCGCCAG GTCCCTGCCA TGGAGGCTGA GCTGCTGGTG 
TCTGGTGATG AAAACTGCGC CTACTTCGAG GTGTCAGCCA AGAAGAACAC TAATGTGAAT 
GAGATGTTCT ATGTGCTGTT CAGCATGGCC AAGCTGCCCC ATGAGATGAG CCCTGCACTG 
CACCATAAGA TCTCCGTGCA GTACGGCGAT GCTTTTCACC CCCGGCCCTT CTGCATGCGT 
CGCACTAAGG TCGCAGGTGC CTATGGCATG GTCTCACCCT TTGCCCGACG CCCCAGTGTC 
AACAGTGACC TCAAGTACAT CAAGGCCAAG GTCCTACGGG AGGGCCAGGC CCGAGAGAGG 
GACAAGTGTA GCATCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083458.1
CDS	145..945
Translation	MMKTLSSGNCTLNVPAKNSYRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKVYNIHGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDSRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVICGNKNDHSELCRQVPAMEAELLVSGDENCAYFEVSAKKNTNVNEMFYVLFSMAKLPHEMSPALHHKISVQYGDAFHPRPFCMRRTKVAGAYGMVSPFARRPSVNSDLKYIKAKVLREGQARERDKCSIQ

Target ORF information:

RefSeq Version	NM_029182.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RASD family, member 2 (Rasd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029182.1

ORF Insert Sequence:

ATGATGAAGA CCTTGTCCAG TGGGAACTGC ACACTCAATG TGCCTGCTAA GAACTCCTAC 
CGCATGGTGG TGCTGGGTGC CTCCCGAGTG GGCAAGAGCT CCATTGTCTC CCGCTTCCTC 
AATGGCCGCT TTGAGGACCA GTACACGCCC ACTATCGAGG ACTTTCATCG CAAGGTGTAC 
AACATCCACG GGGACATGTA CCAGCTGGAT ATCCTGGACA CCTCTGGCAA CCACCCATTC 
CCTGCCATGC GCCGGCTCTC CATCCTCACA GGAGATGTCT TCATCCTGGT GTTCAGCCTG 
GATAGCCGGG AGTCCTTTGA TGAGGTCAAG CGCCTCCAGA AACAGATCCT GGAGGTCAAG 
TCCTGCCTGA AGAATAAAAC CAAGGAGGCA GCAGAGCTGC CCATGGTGAT CTGTGGGAAC 
AAGAATGACC ACAGTGAGCT GTGCCGCCAG GTCCCTGCCA TGGAGGCTGA GCTGCTGGTG 
TCTGGTGATG AAAACTGCGC CTACTTCGAG GTGTCAGCCA AGAAGAACAC TAATGTGAAT 
GAGATGTTCT ATGTGCTGTT CAGCATGGCC AAGCTGCCCC ATGAGATGAG CCCTGCACTG 
CACCATAAGA TCTCCGTGCA GTACGGCGAT GCTTTTCACC CCCGGCCCTT CTGCATGCGT 
CGCACTAAGG TCGCAGGTGC CTATGGCATG GTCTCACCCT TTGCCCGACG CCCCAGTGTC 
AACAGTGACC TCAAGTACAT CAAGGCCAAG GTCCTACGGG AGGGCCAGGC CCGAGAGAGG 
GACAAGTGTA GCATCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An Atoh1-S193A Phospho-Mutant Allele Causes Hearing Deficits and Motor Impairment.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(36)8583-8594(2017 09)
Xie WR,Jen HI,Seymour ML,Yeh SY,Pereira FA,Groves AK,Klisch TJ,Zoghbi HY

Genome-wide Analysis of RAR? Transcriptional Targets in Mouse Striatum Links Retinoic Acid Signaling with Huntington's Disease and Other Neurodegenerative Disorders.
Molecular neurobiology54(5)3859-3878(2017 Jul)
Niewiadomska-Cimicka A,Krzy?osiak A,Ye T,Podle?ny-Drabiniok A,Demb?l? D,Doll? P,Kr??el W

RasGRP1 promotes amphetamine-induced motor behavior through a Rhes interaction network ("Rhesactome") in the striatum.
Science signaling9(454)ra111(2016 Nov)
Shahani N,Swarnkar S,Giovinazzo V,Morgenweck J,Bohn LM,Scharager-Tapia C,Pascal B,Martinez-Acedo P,Khare K,Subramaniam S

Genetic deletion of Rhes or pharmacological blockade of mTORC1 prevent striato-nigral neurons activation in levodopa-induced dyskinesia.
Neurobiology of disease85155-163(2016 Jan)
Brugnoli A,Napolitano F,Usiello A,Morari M