Nkx6-3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nkx6-3
Entrez Gene ID	74561
Full Name	NK6 homeobox 3
Synonyms	9130417I07Rik,Nkx6.3
General protein information	Preferred Names NK6 homeobox 3 Names homeobox protein Nkx-6.3 NK6 transcription factor related, locus 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_029002.2	NP_083278.1	homeobox protein Nkx-6.3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NKX6-3	XP_849158.1
Gallus gallus (chicken)	NKX6-3	XP_428808.2
Pan troglodytes (chimpanzee)	NKX6-3	XP_001138968.1
Mus musculus (house mouse)	Nkx6-3	NP_083278.1
Rattus norvegicus (Norway rat)	Nkx6-3	NP_001102925.1
Danio rerio (zebrafish)	nkx6.3	NP_001159612.1
Xenopus tropicalis (tropical clawed frog)	nkx6-3	XP_002932753.1
Bos taurus (cattle)	LOC784372	XP_002698770.1

Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Arx is required for normal enteroendocrine cell development in mice and humans.
Du A, McCracken KW, Walp ER, Terry NA, Klein TJ, Han A, Wells JM, May CL
Developmental biology365(1)175-88(2012 May)

Screening large numbers of expression patterns of transcription factors in late stages of the mouse thymus.
Chung YC, Tsai YJ, Shiu TY, Sun YY, Wang PF, Chen CL
Gene expression patterns : GEP11(1-2)84-92(2011 Jan-Feb)

Nkx6 transcription factors and Ptf1a function as antagonistic lineage determinants in multipotent pancreatic progenitors.
Schaffer AE, Freude KK, Nelson SB, Sander M
Developmental cell18(6)1022-9(2010 Jun)

Sfrp controls apicobasal polarity and oriented cell division in developing gut epithelium.
Matsuyama M, Aizawa S, Shimono A
PLoS genetics5(3)e1000427(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, cross-antagonism between Nkx6 and Ptf1a in multipotent progenitors governs the equilibrium between endocrine and acinar cell neogenesis required for normal pancreas development.
Title: Nkx6 transcription factors and Ptf1a function as antagonistic lineage determinants in multipotent pancreatic progenitors.

Nkx6.3 function is dispensable for CNS development and lead to the proposal that absence of overt defects is due to functional compensation from a related homeodomain transcription factor.
Title: Expression and function of Nkx6.3 in vertebrate hindbrain.

Nkx6.3 transcripts localize in the epithelium of the most distal stomach region, the antrum and pylorus; expression in the adult intestine is lower and confined to the proximal duodenum.
Title: Requirement of the tissue-restricted homeodomain transcription factor Nkx6.3 in differentiation of gastrin-producing G cells in the stomach antrum.

Nkx6.3 shows a remarkably selective expression in a subpopulation of differentiating V2 neurons at caudal hindbrain levels.
Title: Cloning and analysis of Nkx6.3 during CNS and gastrointestinal development.

The following Nkx6-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nkx6-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06416	NM_029002.2 Latest version!	Mus musculus NK6 homeobox 3 (Nkx6-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nkx6-3 ( NM_029002.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083278.1 Mus musculus NK6 homeobox 3 (Nkx6-3), mRNA.

Price & Availability↑

CloneID	OMu06416
Clone ID Related Accession (Same CDS sequence)	NM_029002.2
Accession Version	NM_029002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Mus musculus(house mouse)
Product	homeobox protein Nkx-6.3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126445.5, AK018683.1 and AK147163.1. On Apr 28, 2009 this sequence version replaced NM_029002.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK147163.1, AK018683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCCA ACCTGCAGGG GACCTTCCTC CTGAACAACA CACAGCTGGC CCAGTTCTCA 
GAAATGAAGG CTCCCATGTG CCAGTACTCG GTGCAGAACT CTTTCTACAA GCTCAGCCCT 
CCGGGACTTG GCCCCCAGCT GGCGGCTGGG ACTCCCCATG GAATCACTGA CATCCTGAGC 
AGACCGGTTG CCACCCCGAA CAGCAGCCTC CTCTCTGGCT ACCCCCACGT GGCAGGCTTT 
GGTGGACTCA GCTCCCAGGG CGTCTACTAT GGCCCCCAGG TGGGGAGCTT TTCCAAGGCT 
GGAAATGAGT ACCCGACCCG GACCCGGAAC TGCTGGGCAG ACACAGGCCA GGACTGGAGA 
GGCAGCGCTC GGCCATGCAG CAACACTCCA GACCCCCTGA GTGACACTAT CCACAAGAAA 
AAGCACACCC GCCCTACCTT CACGGGCCAC CAGATCTTTG CCCTGGAGAA AACCTTTGAG 
CAGACCAAGT ACTTGGCTGG CCCAGAGCGG GCGAGGCTGG CTTATTCCCT GGGCATGACG 
GAGTCACAGG TCAAGGTGTG GTTCCAGAAC CGCCGGACCA AGTGGAGGAA GAAGAGCGCC 
CTGGAGCCCT CATCCTCCAC ACCCCGGGCC CCGGGAGGTG CGAGCGGGGA CCGCGCGGCC 
TCGGAGAACG AGGACGATGA GTACAACAAG CCGCTGGACC CGGACTCGGA CGACGAGAAG 
ATCCGCCTGC TGCTGCGCAA GCACCGGGCC GCCTTCTCCG TGCTCAGCCT GGGCGCGCAC 
AGCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083278.1
CDS	315..1103
Translation	MESNLQGTFLLNNTQLAQFSEMKAPMCQYSVQNSFYKLSPPGLGPQLAAGTPHGITDILSRPVATPNSSLLSGYPHVAGFGGLSSQGVYYGPQVGSFSKAGNEYPTRTRNCWADTGQDWRGSARPCSNTPDPLSDTIHKKKHTRPTFTGHQIFALEKTFEQTKYLAGPERARLAYSLGMTESQVKVWFQNRRTKWRKKSALEPSSSTPRAPGGASGDRAASENEDDEYNKPLDPDSDDEKIRLLLRKHRAAFSVLSLGAHSV

Target ORF information:

RefSeq Version	NM_029002.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NK6 homeobox 3 (Nkx6-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029002.2

ORF Insert Sequence:

ATGGAGTCCA ACCTGCAGGG GACCTTCCTC CTGAACAACA CACAGCTGGC CCAGTTCTCA 
GAAATGAAGG CTCCCATGTG CCAGTACTCG GTGCAGAACT CTTTCTACAA GCTCAGCCCT 
CCGGGACTTG GCCCCCAGCT GGCGGCTGGG ACTCCCCATG GAATCACTGA CATCCTGAGC 
AGACCGGTTG CCACCCCGAA CAGCAGCCTC CTCTCTGGCT ACCCCCACGT GGCAGGCTTT 
GGTGGACTCA GCTCCCAGGG CGTCTACTAT GGCCCCCAGG TGGGGAGCTT TTCCAAGGCT 
GGAAATGAGT ACCCGACCCG GACCCGGAAC TGCTGGGCAG ACACAGGCCA GGACTGGAGA 
GGCAGCGCTC GGCCATGCAG CAACACTCCA GACCCCCTGA GTGACACTAT CCACAAGAAA 
AAGCACACCC GCCCTACCTT CACGGGCCAC CAGATCTTTG CCCTGGAGAA AACCTTTGAG 
CAGACCAAGT ACTTGGCTGG CCCAGAGCGG GCGAGGCTGG CTTATTCCCT GGGCATGACG 
GAGTCACAGG TCAAGGTGTG GTTCCAGAAC CGCCGGACCA AGTGGAGGAA GAAGAGCGCC 
CTGGAGCCCT CATCCTCCAC ACCCCGGGCC CCGGGAGGTG CGAGCGGGGA CCGCGCGGCC 
TCGGAGAACG AGGACGATGA GTACAACAAG CCGCTGGACC CGGACTCGGA CGACGAGAAG 
ATCCGCCTGC TGCTGCGCAA GCACCGGGCC GCCTTCTCCG TGCTCAGCCT GGGCGCGCAC 
AGCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Arx is required for normal enteroendocrine cell development in mice and humans.
Developmental biology365(1)175-88(2012 May)
Du A,McCracken KW,Walp ER,Terry NA,Klein TJ,Han A,Wells JM,May CL

Screening large numbers of expression patterns of transcription factors in late stages of the mouse thymus.
Gene expression patterns : GEP11(1-2)84-92(2011 Jan-Feb)
Chung YC,Tsai YJ,Shiu TY,Sun YY,Wang PF,Chen CL

Nkx6 transcription factors and Ptf1a function as antagonistic lineage determinants in multipotent pancreatic progenitors.
Developmental cell18(6)1022-9(2010 Jun)
Schaffer AE,Freude KK,Nelson SB,Sander M

Sfrp controls apicobasal polarity and oriented cell division in developing gut epithelium.
PLoS genetics5(3)e1000427(2009 Mar)
Matsuyama M,Aizawa S,Shimono A