Plekhf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plekhf1
Entrez Gene ID	72287
Full Name	pleckstrin homology domain containing, family F (with FYVE domain) member 1
Synonyms	1810013P09Rik,APPD,LAPF,PHAFIN1,ZFYVE15
General protein information	Preferred Names pleckstrin homology domain containing, family F (with FYVE domain) member 1 Names pleckstrin homology domain-containing family F member 1 PH domain-containing family F member 1 lysosome-associated apoptosis-inducing protein containing PH and FYVE domains
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_024413.2	NP_077724.2	pleckstrin homology domain-containing family F member 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLEKHF1	NP_077286.3
Pan troglodytes (chimpanzee)	PLEKHF1	XP_001149430.1
	PLEKHF1	XP_001084522.1
Rattus norvegicus (Norway rat)	Plekhf1	NP_001013166.1
Bos taurus (cattle)	PLEKHF1	NP_001075937.1
Mus musculus (house mouse)	Plekhf1	NP_077724.2
Xenopus tropicalis (tropical clawed frog)	plekhf1	NP_001016010.1
Gallus gallus (chicken)	PLEKHF1	XP_004944264.1
Canis lupus familiaris (dog)	PLEKHF1	XP_005616858.1

Gene Ontology
Bibliography

Related articles in PubMed

A global genomic transcriptional code associated with CNS-expressed genes.
Bailey PJ, Klos JM, Andersson E, Karl?n M, K?llstr?m M, Ponjavic J, Muhr J, Lenhard B, Sandelin A, Ericson J
Experimental cell research312(16)3108-19(2006 Oct)

The lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF), representative of a novel family of PH and FYVE domain-containing proteins, induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway.
Chen W, Li N, Chen T, Han Y, Li C, Wang Y, He W, Zhang L, Wan T, Cao X
The Journal of biological chemistry280(49)40985-95(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Plekhf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plekhf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08174	NM_024413.2 Latest version!	Mus musculus pleckstrin homology domain containing, family F (with FYVE domain) member 1 (Plekhf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plekhf1 ( NM_024413.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077724.2 Mus musculus pleckstrin homology domain containing, family F (with FYVE domain) member 1 (Plekhf1), mRNA.

Price & Availability↑

CloneID	OMu08174
Clone ID Related Accession (Same CDS sequence)	NM_024413.2
Accession Version	NM_024413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	pleckstrin homology domain-containing family F member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW227287.1 and AK143976.1. On Mar 21, 2008 this sequence version replaced NM_024413.1. ##Evidence-Data-START## Transcript exon combination :: AW227287.1, AK143976.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGACC ACTTGGCAAA CACGGAGATC AACAGCCAGC GGATTGCAGC AGTGGAGAGC 
TGTTTTGGGG CATCGGGGCA ACCGCTGGCC CTGCCCGGCC GTGTGCTCCT AGGCGAGGGG 
GTACTGACCA AGGAGTGCCG CAAGAAGGCC AAGCCACGCA TCTTCTTTCT TTTCAACGAC 
ATCCTGGTGT ATGGCAGTAT TGTGCTTAGC AAGCGCAAGT ACCGCAGCCA GCATATTATT 
CCCCTAGAAG AGGTAACATT GGAGCCACTG CCCGAGACCC TGCAGGCCAA GAACCGATGG 
ATGATCAAGA CAGCCAAGAA GTCCTTTGTG GTGTCGGCAG CCTCCACCAC GGAGCGCCAG 
GAATGGATAA GCCACATCGA AGAGTGTGTG CGGAGGCAGC TACTGGCCAC AGGCCGCCAG 
CCCACCACGG AGCACGCAGC TCCCTGGATC CCGGACAAGG CCACGGACAT TTGCATGCGC 
TGCACGCAGA CGCGCTTCTC CGCACTCACC CGGCGTCACC ACTGCCGCAA ATGCGGGTTT 
GTGGTCTGTG CTGAGTGTTC CAGGGAGCGT TTCCTGTTGC CACGTCTCTC TCCCAAGCCC 
CTTCGCGTCT GCAGCCTGTG CTACCGAGAG CTGGCCGCCC AGAAGCTGAG GGAGGAGGCC 
AGGGAGGGGA TCGGAGGCTC TCCACCGCAG CTGTCCCACC TAGGCGGCAC TGTCTGTGGT 
GCATCCAGTG GCGATGACGA TGACTCCGAT GAGGACAGAG AGGGCAATGG AGATGGTGAC 
TGGCCCACCC AGGTGGAGTT CTATGCTTCT GGTGTCTCCT GGTCAGCCTT CCATAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077724.2
CDS	71..910
Translation	MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLSKRKYRSQHIIPLEEVTLEPLPETLQAKNRWMIKTAKKSFVVSAASTTERQEWISHIEECVRRQLLATGRQPTTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRERFLLPRLSPKPLRVCSLCYRELAAQKLREEAREGIGGSPPQLSHLGGTVCGASSGDDDDSDEDREGNGDGDWPTQVEFYASGVSWSAFHS

Target ORF information:

RefSeq Version	NM_024413.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pleckstrin homology domain containing, family F (with FYVE domain) member 1 (Plekhf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024413.2

ORF Insert Sequence:

ATGGTGGACC ACTTGGCAAA CACGGAGATC AACAGCCAGC GGATTGCAGC AGTGGAGAGC 
TGTTTTGGGG CATCGGGGCA ACCGCTGGCC CTGCCCGGCC GTGTGCTCCT AGGCGAGGGG 
GTACTGACCA AGGAGTGCCG CAAGAAGGCC AAGCCACGCA TCTTCTTTCT TTTCAACGAC 
ATCCTGGTGT ATGGCAGTAT TGTGCTTAGC AAGCGCAAGT ACCGCAGCCA GCATATTATT 
CCCCTAGAAG AGGTAACATT GGAGCCACTG CCCGAGACCC TGCAGGCCAA GAACCGATGG 
ATGATCAAGA CAGCCAAGAA GTCCTTTGTG GTGTCGGCAG CCTCCACCAC GGAGCGCCAG 
GAATGGATAA GCCACATCGA AGAGTGTGTG CGGAGGCAGC TACTGGCCAC AGGCCGCCAG 
CCCACCACGG AGCACGCAGC TCCCTGGATC CCGGACAAGG CCACGGACAT TTGCATGCGC 
TGCACGCAGA CGCGCTTCTC CGCACTCACC CGGCGTCACC ACTGCCGCAA ATGCGGGTTT 
GTGGTCTGTG CTGAGTGTTC CAGGGAGCGT TTCCTGTTGC CACGTCTCTC TCCCAAGCCC 
CTTCGCGTCT GCAGCCTGTG CTACCGAGAG CTGGCCGCCC AGAAGCTGAG GGAGGAGGCC 
AGGGAGGGGA TCGGAGGCTC TCCACCGCAG CTGTCCCACC TAGGCGGCAC TGTCTGTGGT 
GCATCCAGTG GCGATGACGA TGACTCCGAT GAGGACAGAG AGGGCAATGG AGATGGTGAC 
TGGCCCACCC AGGTGGAGTT CTATGCTTCT GGTGTCTCCT GGTCAGCCTT CCATAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A global genomic transcriptional code associated with CNS-expressed genes.
Experimental cell research312(16)3108-19(2006 Oct)
Bailey PJ,Klos JM,Andersson E,Karl?n M,K?llstr?m M,Ponjavic J,Muhr J,Lenhard B,Sandelin A,Ericson J

The lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF), representative of a novel family of PH and FYVE domain-containing proteins, induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway.
The Journal of biological chemistry280(49)40985-95(2005 Dec)
Chen W,Li N,Chen T,Han Y,Li C,Wang Y,He W,Zhang L,Wan T,Cao X