Pdia6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdia6
Entrez Gene ID	71853
Full Name	protein disulfide isomerase associated 6
Synonyms	1700015E05Rik,AL023058,C77895,CaBP5,P5,Txndc7
General protein information	Preferred Names protein disulfide isomerase associated 6 Names protein disulfide-isomerase A6 protein disulfide isomerase-related protein thioredoxin domain containing 7 thioredoxin domain-containing protein 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_027959.3	NP_082235.1	protein disulfide-isomerase A6 precursor
NM_027959.4	NP_082235.2	protein disulfide-isomerase A6 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PDIA6	XP_532876.2
Mus musculus (house mouse)	Pdia6	NP_082235.1
Arabidopsis thaliana (thale cress)	PDIL2-3	NP_180851.1
Arabidopsis thaliana (thale cress)	PDIL2-2	NP_171990.3
Rattus norvegicus (Norway rat)	Pdia6	NP_001004442.1
Drosophila melanogaster (fruit fly)	CaBP1	NP_609792.1
Caenorhabditis elegans (roundworm)	tag-320	NP_509190.1
Xenopus tropicalis (tropical clawed frog)	pdia6	NP_001007974.1
Danio rerio (zebrafish)	pdip5	NP_922915.2
Oryza sativa (rice)	Os09g0451500	NP_001063331.1
Homo sapiens (human)	PDIA6	NP_005733.1
Pan troglodytes (chimpanzee)	PDIA6	NP_001233576.1
	PDIA6	XP_001095159.2
Bos taurus (cattle)	PDIA6	NP_001193274.1
Gallus gallus (chicken)	PDIA6	XP_419952.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001919	XP_321144.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

A prominent role of PDIA6 in processing of misfolded proinsulin.
Gorasia DG, Dudek NL, Safavi-Hemami H, Perez RA, Schittenhelm RB, Saunders PM, Wee S, Mangum JE, Hubbard MJ, Purcell AW
Biochimica et biophysica acta1864(6)715-723(2016 Jun)

Endoplasmic reticulum resident protein 44 (ERp44) deficiency in mice and zebrafish leads to cardiac developmental and functional defects.
Wang DY, Abbasi C, El-Rass S, Li JY, Dawood F, Naito K, Sharma P, Bousette N, Singh S, Backx PH, Cox B, Wen XY, Liu PP, Gramolini AO
Journal of the American Heart Association3(5)e001018(2014 Oct)

Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis.
Groenendyk J, Peng Z, Dudek E, Fan X, Mizianty MJ, Dufey E, Urra H, Sepulveda D, Rojas-Rivera D, Lim Y, Kim DH, Baretta K, Srikanth S, Gwack Y, Ahnn J, Kaufman RJ, Lee SK, Hetz C, Kurgan L, Michalak M
Science signaling7(329)ra54(2014 Jun)

Protein disulfide isomerase A6 controls the decay of IRE1? signaling via disulfide-dependent association.
Eletto D, Eletto D, Dersh D, Gidalevitz T, Argon Y
Molecular cell53(4)562-576(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

one additional protein, namely P58(IPK), specifically precipitated with Akita proinsulin and approximately ten fold more PDIA6, but not other PDI family members, was bound to Akita proinsulin. The latter suggests that PDIA6 may act as a key reductase
Title: A prominent role of PDIA6 in processing of misfolded proinsulin.

these findings demonstrated that ER Ca2+, PDIA6, IRE1alpha, and miR-322 function in a dynamic feedback loop modulating the UPR under conditions of disrupted ER Ca2+ homeostasis.
Title: Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis.

By facilitating disulfide bond formation, and enhanced ER protein folding, PDIA6 may contribute to the protective effects of ATF6 in the ischemic mouse heart.
Title: Protein disulfide isomerase-associated 6 is an ATF6-inducible ER stress response protein that protects cardiac myocytes from ischemia/reperfusion-mediated cell death.

mitochondrial P5 may upregulate tricarboxylic acid cycle and possibly, other intramitochondrial metabolism.
Title: Mitochondrial P5, a member of protein disulphide isomerase family, suppresses oxidative stress-induced cell death.

The following Pdia6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdia6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17463	NM_027959.3 Latest version!	Mus musculus protein disulfide isomerase associated 6 (Pdia6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu84539	NM_027959.4 Latest version!	Mus musculus protein disulfide isomerase associated 6 (Pdia6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdia6 ( NM_027959.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082235.1 Mus musculus protein disulfide isomerase associated 6 (Pdia6), mRNA.

Price & Availability↑

CloneID	OMu17463
Clone ID Related Accession (Same CDS sequence)	NM_027959.3
Accession Version	NM_027959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Mus musculus(house mouse)
Product	protein disulfide-isomerase A6 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC159815.3. On Apr 30, 2007 this sequence version replaced NM_027959.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK076558.1, SRR1660811.55356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGGGTGA TCGGCATGGC TCGCCTGGTG CTCGGTCTGG TGAGCTGCAC CTTCTTTCTA 
GCAGTCAGCG GTCTGTATTC TTCTAGTGAT GATGTCATCG AGTTGACGCC ATCAAATTTC 
AACAGAGAAG TTATTCAGAG TGACGGCCTG TGGCTTGTAG AATTTTATGC ACCATGGTGT 
GGTCACTGCC AGAGGTTAAC ACCAGAATGG AAGAAAGCAG CAACGGCTTT GAAAGATGTT 
GTTAAAGTCG GGGCCGTCAA TGCAGACAAA CATCAGTCCC TGGGAGGTCA GTATGGTGTC 
CAGGGATTTC CTACCATCAA GATATTTGGA GCTAACAAAA ACAAGCCAGA AGATTATCAG 
GGTGGCAGAA CGGGAGAAGC CATTGTAGAT GCCGCCCTCA GTGCCTTGCG CCAGCTCGTC 
AAGGATCGCC TTGGTGGGCG GAGTGGTGGG TACAGTTCTG GAAAGCAGGG CAGAGGTGAT 
AGTTCAAGTA AGAAAGACGT GGTAGAGCTG ACGGATGACA CCTTTGATAA GAATGTCCTT 
GACAGTGAAG ACGTTTGGAT GGTTGAGTTT TATGCTCCGT GGTGTGGACA CTGCAAAAAC 
CTGGAGCCAG AATGGGCTGC TGCGGCTACA GAAGTAAAGG AGCAAACAAA GGGAAAAGTG 
AAACTGGCAG CTGTGGATGC CACCATGAAT CAGGTTCTGG CCAGCCGATA CGGGATTAAA 
GGATTCCCTA CAATCAAGAT ATTTCAGAAA GGCGAGTCTC CTGTGGACTA TGATGGTGGT 
CGGACGAGAT CTGACATAGT GTCAAGGGCC CTCGATTTGT TCTCTGATAA TGCCCCTCCT 
CCAGAGCTGC TTGAGATAAT CAACGAAGAC ATAGCCAAGA AGACATGCGA GGAGCACCAG 
CTCTGTGTCG TGGCCGTACT GCCTCACATC CTGGACACTG GAGCTGCAGG CAGAAACTCT 
TACTTGGAAG TTCTTCTGAA ACTGGCTGAC AAGTACAAGA AGAAGATGTG GGGGTGGCTG 
TGGACAGAAG CTGGAGCCCA GTATGAACTG GAGAACGCAC TAGGGATTGG AGGGTTCGGG 
TACCCTGCCA TGGCAGCCAT CAATGCACGC AAGATGAAAT TTGCTCTTCT CAAGGGGTCT 
TTCAGTGAAC AAGGCATCAA TGAGTTTCTC AGGGAACTGT CTTTCGGGCG TGGCTCCACA 
GCACCCGTAG GGGGTGGTTC CTTTCCTACC ATCACTCCCA GGGAGCCCTG GGATGGCAAG 
GATGGTGAGC TCCCTGTGGA GGACGACATT GACCTCAGTG ACGTGGAGCT GGATGACCTG 
GAGAAGGATG AGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082235.1
CDS	24..1361
Translation	MRVIGMARLVLGLVSCTFFLAVSGLYSSSDDVIELTPSNFNREVIQSDGLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVNADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRGDSSSKKDVVELTDDTFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAATEVKEQTKGKVKLAAVDATMNQVLASRYGIKGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKKTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQYELENALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGSFPTITPREPWDGKDGELPVEDDIDLSDVELDDLEKDEL

Target ORF information:

RefSeq Version	NM_027959.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein disulfide isomerase associated 6 (Pdia6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027959.3

ORF Insert Sequence:

ATGCGGGTGA TCGGCATGGC TCGCCTGGTG CTCGGTCTGG TGAGCTGCAC CTTCTTTCTA 
GCAGTCAGCG GTCTGTATTC TTCTAGTGAT GATGTCATCG AGTTGACGCC ATCAAATTTC 
AACAGAGAAG TTATTCAGAG TGACGGCCTG TGGCTTGTAG AATTTTATGC ACCATGGTGT 
GGTCACTGCC AGAGGTTAAC ACCAGAATGG AAGAAAGCAG CAACGGCTTT GAAAGATGTT 
GTTAAAGTCG GGGCCGTCAA TGCAGACAAA CATCAGTCCC TGGGAGGTCA GTATGGTGTC 
CAGGGATTTC CTACCATCAA GATATTTGGA GCTAACAAAA ACAAGCCAGA AGATTATCAG 
GGTGGCAGAA CGGGAGAAGC CATTGTAGAT GCCGCCCTCA GTGCCTTGCG CCAGCTCGTC 
AAGGATCGCC TTGGTGGGCG GAGTGGTGGG TACAGTTCTG GAAAGCAGGG CAGAGGTGAT 
AGTTCAAGTA AGAAAGACGT GGTAGAGCTG ACGGATGACA CCTTTGATAA GAATGTCCTT 
GACAGTGAAG ACGTTTGGAT GGTTGAGTTT TATGCTCCGT GGTGTGGACA CTGCAAAAAC 
CTGGAGCCAG AATGGGCTGC TGCGGCTACA GAAGTAAAGG AGCAAACAAA GGGAAAAGTG 
AAACTGGCAG CTGTGGATGC CACCATGAAT CAGGTTCTGG CCAGCCGATA CGGGATTAAA 
GGATTCCCTA CAATCAAGAT ATTTCAGAAA GGCGAGTCTC CTGTGGACTA TGATGGTGGT 
CGGACGAGAT CTGACATAGT GTCAAGGGCC CTCGATTTGT TCTCTGATAA TGCCCCTCCT 
CCAGAGCTGC TTGAGATAAT CAACGAAGAC ATAGCCAAGA AGACATGCGA GGAGCACCAG 
CTCTGTGTCG TGGCCGTACT GCCTCACATC CTGGACACTG GAGCTGCAGG CAGAAACTCT 
TACTTGGAAG TTCTTCTGAA ACTGGCTGAC AAGTACAAGA AGAAGATGTG GGGGTGGCTG 
TGGACAGAAG CTGGAGCCCA GTATGAACTG GAGAACGCAC TAGGGATTGG AGGGTTCGGG 
TACCCTGCCA TGGCAGCCAT CAATGCACGC AAGATGAAAT TTGCTCTTCT CAAGGGGTCT 
TTCAGTGAAC AAGGCATCAA TGAGTTTCTC AGGGAACTGT CTTTCGGGCG TGGCTCCACA 
GCACCCGTAG GGGGTGGTTC CTTTCCTACC ATCACTCCCA GGGAGCCCTG GGATGGCAAG 
GATGGTGAGC TCCCTGTGGA GGACGACATT GACCTCAGTG ACGTGGAGCT GGATGACCTG 
GAGAAGGATG AGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdia6 ( NM_027959.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082235.2 Mus musculus protein disulfide isomerase associated 6 (Pdia6), mRNA.

Price & Availability↑

CloneID	OMu84539
Clone ID Related Accession (Same CDS sequence)	NM_027959.4
Accession Version	NM_027959.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	protein disulfide-isomerase A6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159815.3. On Feb 20, 2019 this sequence version replaced NM_027959.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189670.104074.1, SRR3224858.2071.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGCTCGCC TGGTGCTCGG TCTGGTGAGC TGCACCTTCT TTCTAGCAGT CAGCGGTCTG 
TATTCTTCTA GTGATGATGT CATCGAGTTG ACGCCATCAA ATTTCAACAG AGAAGTTATT 
CAGAGTGACG GCCTGTGGCT TGTAGAATTT TATGCACCAT GGTGTGGTCA CTGCCAGAGG 
TTAACACCAG AATGGAAGAA AGCAGCAACG GCTTTGAAAG ATGTTGTTAA AGTCGGGGCC 
GTCAATGCAG ACAAACATCA GTCCCTGGGA GGTCAGTATG GTGTCCAGGG ATTTCCTACC 
ATCAAGATAT TTGGAGCTAA CAAAAACAAG CCAGAAGATT ATCAGGGTGG CAGAACGGGA 
GAAGCCATTG TAGATGCCGC CCTCAGTGCC TTGCGCCAGC TCGTCAAGGA TCGCCTTGGT 
GGGCGGAGTG GTGGGTACAG TTCTGGAAAG CAGGGCAGAG GTGATAGTTC AAGTAAGAAA 
GACGTGGTAG AGCTGACGGA TGACACCTTT GATAAGAATG TCCTTGACAG TGAAGACGTT 
TGGATGGTTG AGTTTTATGC TCCGTGGTGT GGACACTGCA AAAACCTGGA GCCAGAATGG 
GCTGCTGCGG CTACAGAAGT AAAGGAGCAA ACAAAGGGAA AAGTGAAACT GGCAGCTGTG 
GATGCCACCA TGAATCAGGT TCTGGCCAGC CGATACGGGA TTAAAGGATT CCCTACAATC 
AAGATATTTC AGAAAGGCGA GTCTCCTGTG GACTATGATG GTGGTCGGAC GAGATCTGAC 
ATAGTGTCAA GGGCCCTCGA TTTGTTCTCT GATAATGCCC CTCCTCCAGA GCTGCTTGAG 
ATAATCAACG AAGACATAGC CAAGAAGACA TGCGAGGAGC ACCAGCTCTG TGTCGTGGCC 
GTACTGCCTC ACATCCTGGA CACTGGAGCT GCAGGCAGAA ACTCTTACTT GGAAGTTCTT 
CTGAAACTGG CTGACAAGTA CAAGAAGAAG ATGTGGGGGT GGCTGTGGAC AGAAGCTGGA 
GCCCAGTATG AACTGGAGAA CGCACTAGGG ATTGGAGGGT TCGGGTACCC TGCCATGGCA 
GCCATCAATG CACGCAAGAT GAAATTTGCT CTTCTCAAGG GGTCTTTCAG TGAACAAGGC 
ATCAATGAGT TTCTCAGGGA ACTGTCTTTC GGGCGTGGCT CCACAGCACC CGTAGGGGGT 
GGTTCCTTTC CTACCATCAC TCCCAGGGAG CCCTGGGATG GCAAGGATGG TGAGCTCCCT 
GTGGAGGACG ACATTGACCT CAGTGACGTG GAGCTGGATG ACCTGGAGAA GGATGAGTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082235.2
CDS	45..1367
Translation	MARLVLGLVSCTFFLAVSGLYSSSDDVIELTPSNFNREVIQSDGLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVNADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRGDSSSKKDVVELTDDTFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAATEVKEQTKGKVKLAAVDATMNQVLASRYGIKGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKKTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQYELENALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGSFPTITPREPWDGKDGELPVEDDIDLSDVELDDLEKDEL

Target ORF information:

RefSeq Version	NM_027959.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein disulfide isomerase associated 6 (Pdia6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027959.4

ORF Insert Sequence:

ATGGCTCGCC TGGTGCTCGG TCTGGTGAGC TGCACCTTCT TTCTAGCAGT CAGCGGTCTG 
TATTCTTCTA GTGATGATGT CATCGAGTTG ACGCCATCAA ATTTCAACAG AGAAGTTATT 
CAGAGTGACG GCCTGTGGCT TGTAGAATTT TATGCACCAT GGTGTGGTCA CTGCCAGAGG 
TTAACACCAG AATGGAAGAA AGCAGCAACG GCTTTGAAAG ATGTTGTTAA AGTCGGGGCC 
GTCAATGCAG ACAAACATCA GTCCCTGGGA GGTCAGTATG GTGTCCAGGG ATTTCCTACC 
ATCAAGATAT TTGGAGCTAA CAAAAACAAG CCAGAAGATT ATCAGGGTGG CAGAACGGGA 
GAAGCCATTG TAGATGCCGC CCTCAGTGCC TTGCGCCAGC TCGTCAAGGA TCGCCTTGGT 
GGGCGGAGTG GTGGGTACAG TTCTGGAAAG CAGGGCAGAG GTGATAGTTC AAGTAAGAAA 
GACGTGGTAG AGCTGACGGA TGACACCTTT GATAAGAATG TCCTTGACAG TGAAGACGTT 
TGGATGGTTG AGTTTTATGC TCCGTGGTGT GGACACTGCA AAAACCTGGA GCCAGAATGG 
GCTGCTGCGG CTACAGAAGT AAAGGAGCAA ACAAAGGGAA AAGTGAAACT GGCAGCTGTG 
GATGCCACCA TGAATCAGGT TCTGGCCAGC CGATACGGGA TTAAAGGATT CCCTACAATC 
AAGATATTTC AGAAAGGCGA GTCTCCTGTG GACTATGATG GTGGTCGGAC GAGATCTGAC 
ATAGTGTCAA GGGCCCTCGA TTTGTTCTCT GATAATGCCC CTCCTCCAGA GCTGCTTGAG 
ATAATCAACG AAGACATAGC CAAGAAGACA TGCGAGGAGC ACCAGCTCTG TGTCGTGGCC 
GTACTGCCTC ACATCCTGGA CACTGGAGCT GCAGGCAGAA ACTCTTACTT GGAAGTTCTT 
CTGAAACTGG CTGACAAGTA CAAGAAGAAG ATGTGGGGGT GGCTGTGGAC AGAAGCTGGA 
GCCCAGTATG AACTGGAGAA CGCACTAGGG ATTGGAGGGT TCGGGTACCC TGCCATGGCA 
GCCATCAATG CACGCAAGAT GAAATTTGCT CTTCTCAAGG GGTCTTTCAG TGAACAAGGC 
ATCAATGAGT TTCTCAGGGA ACTGTCTTTC GGGCGTGGCT CCACAGCACC CGTAGGGGGT 
GGTTCCTTTC CTACCATCAC TCCCAGGGAG CCCTGGGATG GCAAGGATGG TGAGCTCCCT 
GTGGAGGACG ACATTGACCT CAGTGACGTG GAGCTGGATG ACCTGGAGAA GGATGAGTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

A prominent role of PDIA6 in processing of misfolded proinsulin.
Biochimica et biophysica acta1864(6)715-723(2016 Jun)
Gorasia DG,Dudek NL,Safavi-Hemami H,Perez RA,Schittenhelm RB,Saunders PM,Wee S,Mangum JE,Hubbard MJ,Purcell AW

Endoplasmic reticulum resident protein 44 (ERp44) deficiency in mice and zebrafish leads to cardiac developmental and functional defects.
Journal of the American Heart Association3(5)e001018(2014 Oct)
Wang DY,Abbasi C,El-Rass S,Li JY,Dawood F,Naito K,Sharma P,Bousette N,Singh S,Backx PH,Cox B,Wen XY,Liu PP,Gramolini AO

Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis.
Science signaling7(329)ra54(2014 Jun)
Groenendyk J,Peng Z,Dudek E,Fan X,Mizianty MJ,Dufey E,Urra H,Sepulveda D,Rojas-Rivera D,Lim Y,Kim DH,Baretta K,Srikanth S,Gwack Y,Ahnn J,Kaufman RJ,Lee SK,Hetz C,Kurgan L,Michalak M

Protein disulfide isomerase A6 controls the decay of IRE1? signaling via disulfide-dependent association.
Molecular cell53(4)562-576(2014 Feb)
Eletto D,Eletto D,Dersh D,Gidalevitz T,Argon Y