Grap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grap
Entrez Gene ID	71520
Full Name	GRB2-related adaptor protein
Synonyms	8430435N19Rik,BB233594
General protein information	Preferred Names GRB2-related adaptor protein Names GRB2-related adapter protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_027817.3	NP_082093.1	GRB2-related adapter protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GRAP	NP_001264482.1
	GRAP	XP_001097817.1
Rattus norvegicus (Norway rat)	Grap	NP_001020920.1
Homo sapiens (human)	GRAP	NP_006604.1
Danio rerio (zebrafish)	grapb	NP_001025251.1
Xenopus tropicalis (tropical clawed frog)	LOC100127598	NP_001106428.1
Canis lupus familiaris (dog)	GRAP	XP_546653.2
Danio rerio (zebrafish)	grapa	NP_001018409.1
Bos taurus (cattle)	GRAP	NP_001095643.1
Mus musculus (house mouse)	Grap	NP_082093.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling.
Cummins TD, Barati MT, Coventry SC, Salyer SA, Klein JB, Powell DW
Biochimica et biophysica acta1804(4)653-61(2010 Apr)

Two distinct tyrosine-based motifs enable the inhibitory receptor FcgammaRIIB to cooperatively recruit the inositol phosphatases SHIP1/2 and the adapters Grb2/Grap.
Isnardi I, Lesourne R, Bruhns P, Fridman WH, Cambier JC, Da?ron M
The Journal of biological chemistry279(50)51931-8(2004 Dec)

Detection of Grb-2-related adaptor protein gene (GRAP) and peptide molecule in salivary glands of MRL/lpr mice and patients with Sj?gren's syndrome.
Shiraiwa H, Takei M, Yoshikawa T, Azuma T, Kato M, Mitamura K, Ueki T, Kida A, Horie T, Seki N, Sawada S
The Journal of international medical research32(3)284-91(2004 May-Jun)

Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction.
Shen R, Ouyang YB, Qu CK, Alonso A, Sperzel L, Mustelin T, Kaplan MH, Feng GS
Molecular and cellular biology22(10)3230-6(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Grb2-related adaptor protein (GRAP) was up-regulated in kidney tubules from diabetic mice, indicating a potential novel role for GRAP in TGF-beta-induced tubule injury in diabetic kidney disease.
Title: Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling.

FcgammaRIIB docking site for the SH2 domain-containing adapters Grb2 and Grap
Title: Two distinct tyrosine-based motifs enable the inhibitory receptor FcgammaRIIB to cooperatively recruit the inositol phosphatases SHIP1/2 and the adapters Grb2/Grap.

Results suggest that the GRAP gene might have a role in the pathogenesis of Sjogren's syndrome.
Title: Detection of Grb-2-related adaptor protein gene (GRAP) and peptide molecule in salivary glands of MRL/lpr mice and patients with Sj?gren's syndrome.

Data suggest that Grap, unlike Grb2, acts as a negative regulator of TCR-stimulated intracellular signaling by downregulating signal relay through the Ras/Erk pathway.
Title: Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction.

The following Grap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04640	NM_027817.3 Latest version!	Mus musculus GRB2-related adaptor protein (Grap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grap ( NM_027817.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082093.1 Mus musculus GRB2-related adaptor protein (Grap), mRNA.

Price & Availability↑

CloneID	OMu04640
Clone ID Related Accession (Same CDS sequence)	NM_027817.3
Accession Version	NM_027817.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	GRB2-related adapter protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL596209.13. On Apr 30, 2007 this sequence version replaced NM_027817.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK018457.1, BC120674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCCG TGGCCCTGTA CAGCTTCCAG GCTACCGAGA GTGACGAGCT GGCCTTCAAC 
AAGGGGGACA CGCTTAAGAT CCTGAACATG GAAGATGACC AGAACTGGTA CAAAGCTGAG 
CTCCGAGGAG CGGAGGGTTT TGTTCCCAAG AACTACATCC GTGTAAAGCC ACACCCGTGG 
TACTCGGGCA GGATCTCTCG ACAGCTGGCT GAAGAGACTC TGATGAAACG CAACCACCTA 
GGAGCCTTCC TGATCAGGGA GAGTGAGAGT TCCCCTGGCG AGTTCTCCGT CTCTGTGAAT 
TATGGTGACC AGGTGCAGCA CTTCAAAGTG CTTCGAGAGG CCTCAGGGAA GTACTTCCTG 
TGGGAGGAGA AGTTCAACTC CCTCAACGAG CTGGTCGACT TCTACCGCAC CACCACCATC 
GCCAAGAGGC GGCAGATCTT CCTGTGTGAC GAGCAACCAC TGATCAAGCC ATCCCGGGCT 
TGCTTTGCCC AGGCCCAGTT TGACTTCTCG GCACAGGATC CATCTCAGCT CAGCCTCCGC 
CGAGGTGACA TCGTGGAAGT TGTGGAGCGT GAGGATCCAC ACTGGTGGCG GGGCCGGGCA 
GGCGGGCGCC TGGGCTTCTT CCCACGGAGC TACGTGCAAC CGGTACACTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082093.1
CDS	27..680
Translation	MESVALYSFQATESDELAFNKGDTLKILNMEDDQNWYKAELRGAEGFVPKNYIRVKPHPWYSGRISRQLAEETLMKRNHLGAFLIRESESSPGEFSVSVNYGDQVQHFKVLREASGKYFLWEEKFNSLNELVDFYRTTTIAKRRQIFLCDEQPLIKPSRACFAQAQFDFSAQDPSQLSLRRGDIVEVVEREDPHWWRGRAGGRLGFFPRSYVQPVHL

Target ORF information:

RefSeq Version	NM_027817.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GRB2-related adaptor protein (Grap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027817.3

ORF Insert Sequence:

ATGGAGTCCG TGGCCCTGTA CAGCTTCCAG GCTACCGAGA GTGACGAGCT GGCCTTCAAC 
AAGGGGGACA CGCTTAAGAT CCTGAACATG GAAGATGACC AGAACTGGTA CAAAGCTGAG 
CTCCGAGGAG CGGAGGGTTT TGTTCCCAAG AACTACATCC GTGTAAAGCC ACACCCGTGG 
TACTCGGGCA GGATCTCTCG ACAGCTGGCT GAAGAGACTC TGATGAAACG CAACCACCTA 
GGAGCCTTCC TGATCAGGGA GAGTGAGAGT TCCCCTGGCG AGTTCTCCGT CTCTGTGAAT 
TATGGTGACC AGGTGCAGCA CTTCAAAGTG CTTCGAGAGG CCTCAGGGAA GTACTTCCTG 
TGGGAGGAGA AGTTCAACTC CCTCAACGAG CTGGTCGACT TCTACCGCAC CACCACCATC 
GCCAAGAGGC GGCAGATCTT CCTGTGTGAC GAGCAACCAC TGATCAAGCC ATCCCGGGCT 
TGCTTTGCCC AGGCCCAGTT TGACTTCTCG GCACAGGATC CATCTCAGCT CAGCCTCCGC 
CGAGGTGACA TCGTGGAAGT TGTGGAGCGT GAGGATCCAC ACTGGTGGCG GGGCCGGGCA 
GGCGGGCGCC TGGGCTTCTT CCCACGGAGC TACGTGCAAC CGGTACACTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling.
Biochimica et biophysica acta1804(4)653-61(2010 Apr)
Cummins TD,Barati MT,Coventry SC,Salyer SA,Klein JB,Powell DW

Two distinct tyrosine-based motifs enable the inhibitory receptor FcgammaRIIB to cooperatively recruit the inositol phosphatases SHIP1/2 and the adapters Grb2/Grap.
The Journal of biological chemistry279(50)51931-8(2004 Dec)
Isnardi I,Lesourne R,Bruhns P,Fridman WH,Cambier JC,Da?ron M

Detection of Grb-2-related adaptor protein gene (GRAP) and peptide molecule in salivary glands of MRL/lpr mice and patients with Sj?gren's syndrome.
The Journal of international medical research32(3)284-91(2004 May-Jun)
Shiraiwa H,Takei M,Yoshikawa T,Azuma T,Kato M,Mitamura K,Ueki T,Kida A,Horie T,Seki N,Sawada S

Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction.
Molecular and cellular biology22(10)3230-6(2002 May)
Shen R,Ouyang YB,Qu CK,Alonso A,Sperzel L,Mustelin T,Kaplan MH,Feng GS