Nolc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nolc1
Entrez Gene ID	70769
Full Name	nucleolar and coiled-body phosphoprotein 1
Synonyms	NOPP130,NOPP140,P130
General protein information	Preferred Names nucleolar and coiled-body phosphoprotein 1 Names nucleolar and coiled-body phosphoprotein 1 140 kDa nucleolar phosphoprotein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_053086.3	NP_444316.2	nucleolar and coiled-body phosphoprotein 1 isoform A
NM_001039353.2	NP_001034442.1	nucleolar and coiled-body phosphoprotein 1 isoform D
NM_001039352.2	NP_001034441.1	nucleolar and coiled-body phosphoprotein 1 isoform C
NM_001039351.2	NP_001034440.1	nucleolar and coiled-body phosphoprotein 1 isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Enhanced NOLC1 promotes cell senescence and represses hepatocellular carcinoma cell proliferation by disturbing the organization of nucleolus.
Yuan F, Zhang Y, Ma L, Cheng Q, Li G, Tong T
Aging cell16(4)726-737(2017 Aug)

Three-Dimensional Distribution of UBF and Nopp140 in Relationship to Ribosomal DNA Transcription During Mouse Preimplantation Development.
Kon? MC, Fleurot R, Chebrout M, Debey P, Beaujean N, Bonnet-Garnier A
Biology of reproduction94(4)95(2016 Apr)

NOLC1, an enhancer of nasopharyngeal carcinoma progression, is essential for TP53 to regulate MDM2 expression.
Hwang YC, Lu TY, Huang DY, Kuo YS, Kao CF, Yeh NH, Wu HC, Lin CT
The American journal of pathology175(1)342-54(2009 Jul)

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
Sansom SN, Griffiths DS, Faedo A, Kleinjan DJ, Ruan Y, Smith J, van Heyningen V, Rubenstein JL, Livesey FJ
PLoS genetics5(6)e1000511(2009 Jun)

Residual Cajal bodies in coilin knockout mice fail to recruit Sm snRNPs and SMN, the spinal muscular atrophy gene product.
Tucker KE, Berciano MT, Jacobs EY, LePage DF, Shpargel KB, Rossire JJ, Chan EK, Lafarga M, Conlon RA, Matera AG
The Journal of cell biology154(2)293-307(2001 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Enhanced NOLC1 promotes cell senescence and represses hepatocellular carcinoma cell proliferation by disturbing the organization of nucleolus.
Title: Enhanced NOLC1 promotes cell senescence and represses hepatocellular carcinoma cell proliferation by disturbing the organization of nucleolus.

UBF and Nopp140 dynamic redistribution between the two-cell and blastocyst stages (time of implantation) is correlated with morphological and structural modifications that occur in embryonic nucleolar compartments.
Title: Three-Dimensional Distribution of UBF and Nopp140 in Relationship to Ribosomal DNA Transcription During Mouse Preimplantation Development.

The following Nolc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nolc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00592	NM_001039351.2 Latest version!	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu00594	NM_001039352.2 Latest version!	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu00595	NM_001039353.2 Latest version!	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu09048	NM_053086.3 Latest version!	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nolc1 ( NM_001039351.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034440.1 Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00592
Clone ID Related Accession (Same CDS sequence)	NM_001039351.2
Accession Version	NM_001039351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	nucleolar and coiled-body phosphoprotein 1 isoform B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY031361.1, AK029150.1, AK147118.1 and BE992617.1. On Nov 7, 2013 this sequence version replaced NM_001039351.1. Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the central coding region, compared to variant 3. The encoded isoform (B) is shorter, compared to isoform C. ##Evidence-Data-START## Transcript exon combination :: AK034817.1, AK029150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAAGTCC 
ACCAAAGCCC CAAAGGTGAA GTTACAGTCA AATGGACCAG TGACCAAGAA GGCTAAGAAA 
GAGACTTCAT CCAGTGACAG CAGTGAGGAC AGCAGTGAGG ACGAGGACAA AAAAGCCCAG 
GGACTTCCCA CACAGAAGGC TGCCGCACAG GTCAAGCGAG CCAGTGTGCC TCAGCATGCT 
GGAAAGGCAG CAGCCAAAGC TTCAGAGAGC AGCAGTAGTG AAGAATCCAG TGAGGAAGAG 
GAAGAGGACA AAAAGAAAAA GCCTGTCCAG AAGGCAGCTA AGCCCCAAGC CAAGGCAGTC 
AGACCTCCTG CGAAGAAGGC AGAGAGCTCT GAGTCGGACT CAGACTCGGA TTCGGACTCC 
AGCTCAGAGG AAGAAACACC ACAGACCCAG AAGCCAAAGG CAGCTGTGGC AGCAAAAGCT 
CAGACTAAAG CCGAAGCCAA ACCAGGTACA CCAGCGAAAG CACAGCCTAA GGTAGCCAAT 
GGCAAAGCAG CCGCCAGCAG CAGCAGCAGC AGCAGCAGCG ATGACTCAGA GGAAGAGAAG 
AAGGCAGCTG CACCTCCCAA GAAGACTGTA CCAAAAAAGC AAGTCGTGGC CAAGGCCCCA 
GTGAAAGTAG CTGCCGCCCC CACCCAGAAG AGCTCCAGCA GTGAGGATTC TTCCAGTGAA 
GAGGAGGAGG GACAGAGACA ACCCATGAAG AAAAAAGCAG GTCCCTACAG TTCAGTTCCA 
CCACCCTCTG TTCCTTTACC AAAGAAGTCC CCGGGAACCC AGGCTCCAAA GAAAGCTGCT 
GCGCAGACAC AGCCTGCAGA CAGCAGTGAC GACAGCAGTG ACGATTCTGA TTCAAGTTCT 
GAGGAAGAGA AAAAACCTCC AGCTAAGACG GTCGTCTCCA AGACACCCGC CAAAGCAGCT 
CCAGTGAAGA AGAAAGCAGA AAGCTCTTCA GACAGCTCGG ATTCTGACAG TTCTGAGGAT 
GAAGCTCCTG CCAAGCCAGT CAGTACAACC AAGAGTCCCA AGCCAGCTGT CACTCCGAAG 
CCATCTGCAG CAAAGGCAGT GACAACTCCT AAGCAACCTG CAGGCAGTAA CCAGAAACCT 
CAGAGCAGGA AGGCTGACAG CAGCTCCAGC GAGGAGGAAA GCAGCTCCAG CGAGGAGGAG 
GAGGCCTCCA AGAAAAGTGC CACAACCCCC AAGGCCAAGG TGACTGCTAA AGCAGCACCC 
GCCAAACAGG CCCCTCAGGC TGCTGGGGAC AGCAGCTCTG ACTCAGATAG TTCCAGCAGT 
GAAGAGGAGG AGAAGACTCC TAAGCCCCCA GCTAAGAAGA AGGCAGCAGG TGGAGCCGTT 
TCTACACCAG CCCCTGGGAA GAAAGCAGAG GCCAAGAGCA GCAGCAGCAG CAGCAGCAGC 
AGCTCCGAAG ATTCCAGTGA AGAGGAGAAA AAAAAGAAGC CCAAAGCTAC TACCCCTAAA 
ATACAGGCAA GCAAGGCCAA TGGCACTCCA GCTTCTCTGA ATGGAAAAGC AGCCAAGGAA 
AGTGAGGAGG AAGAGGAGGA GGAAGAAACA GAAGAGAAGA AAAAGGCAGC TGGGACCAAG 
CCAGGTTCAG GCAAAAAACG GAAGCAGAAT GAGACCGCAG ATGAAGCAAC AACTCCTCAA 
GCTAAGAAAG TTAAGCTCGA GACCCCCAAT ACGTTTCCAA AAAGGAAGAA GGGAGAAAGA 
AGGGCGTCTT CCCCTTTCCG AAGGGTCAGG GAGGAGGAGA TTGAGGTGGA CTCTCGAGTG 
GCGGACAATT CCTTTGATGC CAAGCGAGGT GCAGCTGGAG ACTGGGGGGA GCGAGCCAAT 
CAGGTTCTGA AGTTCACCAA AGGCAAGTCC TTCCGGCATG AAAAAACGAA GAAGAAGCGA 
GGCAGCTACC GGGGAGGCTC CATCTCTGTC CAGGTCAATT CCGTCAAATT CGACAGCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034440.1
CDS	184..2286
Translation	MADTGLRRVVPSDLYPLVLRFLRDSQLSEVASKFAKATGATQQDANASSLLDIYSFWLKSTKAPKVKLQSNGPVTKKAKKETSSSDSSEDSSEDEDKKAQGLPTQKAAAQVKRASVPQHAGKAAAKASESSSSEESSEEEEEDKKKKPVQKAAKPQAKAVRPPAKKAESSESDSDSDSDSSSEEETPQTQKPKAAVAAKAQTKAEAKPGTPAKAQPKVANGKAAASSSSSSSSDDSEEEKKAAAPPKKTVPKKQVVAKAPVKVAAAPTQKSSSSEDSSSEEEEGQRQPMKKKAGPYSSVPPPSVPLPKKSPGTQAPKKAAAQTQPADSSDDSSDDSDSSSEEEKKPPAKTVVSKTPAKAAPVKKKAESSSDSSDSDSSEDEAPAKPVSTTKSPKPAVTPKPSAAKAVTTPKQPAGSNQKPQSRKADSSSSEEESSSSEEEEASKKSATTPKAKVTAKAAPAKQAPQAAGDSSSDSDSSSSEEEEKTPKPPAKKKAAGGAVSTPAPGKKAEAKSSSSSSSSSSEDSSEEEKKKKPKATTPKIQASKANGTPASLNGKAAKESEEEEEEEETEEKKKAAGTKPGSGKKRKQNETADEATTPQAKKVKLETPNTFPKRKKGERRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFDSE

Target ORF information:

RefSeq Version	NM_001039351.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039351.2

ORF Insert Sequence:

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAAGTCC 
ACCAAAGCCC CAAAGGTGAA GTTACAGTCA AATGGACCAG TGACCAAGAA GGCTAAGAAA 
GAGACTTCAT CCAGTGACAG CAGTGAGGAC AGCAGTGAGG ACGAGGACAA AAAAGCCCAG 
GGACTTCCCA CACAGAAGGC TGCCGCACAG GTCAAGCGAG CCAGTGTGCC TCAGCATGCT 
GGAAAGGCAG CAGCCAAAGC TTCAGAGAGC AGCAGTAGTG AAGAATCCAG TGAGGAAGAG 
GAAGAGGACA AAAAGAAAAA GCCTGTCCAG AAGGCAGCTA AGCCCCAAGC CAAGGCAGTC 
AGACCTCCTG CGAAGAAGGC AGAGAGCTCT GAGTCGGACT CAGACTCGGA TTCGGACTCC 
AGCTCAGAGG AAGAAACACC ACAGACCCAG AAGCCAAAGG CAGCTGTGGC AGCAAAAGCT 
CAGACTAAAG CCGAAGCCAA ACCAGGTACA CCAGCGAAAG CACAGCCTAA GGTAGCCAAT 
GGCAAAGCAG CCGCCAGCAG CAGCAGCAGC AGCAGCAGCG ATGACTCAGA GGAAGAGAAG 
AAGGCAGCTG CACCTCCCAA GAAGACTGTA CCAAAAAAGC AAGTCGTGGC CAAGGCCCCA 
GTGAAAGTAG CTGCCGCCCC CACCCAGAAG AGCTCCAGCA GTGAGGATTC TTCCAGTGAA 
GAGGAGGAGG GACAGAGACA ACCCATGAAG AAAAAAGCAG GTCCCTACAG TTCAGTTCCA 
CCACCCTCTG TTCCTTTACC AAAGAAGTCC CCGGGAACCC AGGCTCCAAA GAAAGCTGCT 
GCGCAGACAC AGCCTGCAGA CAGCAGTGAC GACAGCAGTG ACGATTCTGA TTCAAGTTCT 
GAGGAAGAGA AAAAACCTCC AGCTAAGACG GTCGTCTCCA AGACACCCGC CAAAGCAGCT 
CCAGTGAAGA AGAAAGCAGA AAGCTCTTCA GACAGCTCGG ATTCTGACAG TTCTGAGGAT 
GAAGCTCCTG CCAAGCCAGT CAGTACAACC AAGAGTCCCA AGCCAGCTGT CACTCCGAAG 
CCATCTGCAG CAAAGGCAGT GACAACTCCT AAGCAACCTG CAGGCAGTAA CCAGAAACCT 
CAGAGCAGGA AGGCTGACAG CAGCTCCAGC GAGGAGGAAA GCAGCTCCAG CGAGGAGGAG 
GAGGCCTCCA AGAAAAGTGC CACAACCCCC AAGGCCAAGG TGACTGCTAA AGCAGCACCC 
GCCAAACAGG CCCCTCAGGC TGCTGGGGAC AGCAGCTCTG ACTCAGATAG TTCCAGCAGT 
GAAGAGGAGG AGAAGACTCC TAAGCCCCCA GCTAAGAAGA AGGCAGCAGG TGGAGCCGTT 
TCTACACCAG CCCCTGGGAA GAAAGCAGAG GCCAAGAGCA GCAGCAGCAG CAGCAGCAGC 
AGCTCCGAAG ATTCCAGTGA AGAGGAGAAA AAAAAGAAGC CCAAAGCTAC TACCCCTAAA 
ATACAGGCAA GCAAGGCCAA TGGCACTCCA GCTTCTCTGA ATGGAAAAGC AGCCAAGGAA 
AGTGAGGAGG AAGAGGAGGA GGAAGAAACA GAAGAGAAGA AAAAGGCAGC TGGGACCAAG 
CCAGGTTCAG GCAAAAAACG GAAGCAGAAT GAGACCGCAG ATGAAGCAAC AACTCCTCAA 
GCTAAGAAAG TTAAGCTCGA GACCCCCAAT ACGTTTCCAA AAAGGAAGAA GGGAGAAAGA 
AGGGCGTCTT CCCCTTTCCG AAGGGTCAGG GAGGAGGAGA TTGAGGTGGA CTCTCGAGTG 
GCGGACAATT CCTTTGATGC CAAGCGAGGT GCAGCTGGAG ACTGGGGGGA GCGAGCCAAT 
CAGGTTCTGA AGTTCACCAA AGGCAAGTCC TTCCGGCATG AAAAAACGAA GAAGAAGCGA 
GGCAGCTACC GGGGAGGCTC CATCTCTGTC CAGGTCAATT CCGTCAAATT CGACAGCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nolc1 ( NM_001039352.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034441.1 Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu00594
Clone ID Related Accession (Same CDS sequence)	NM_001039352.2
Accession Version	NM_001039352.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	nucleolar and coiled-body phosphoprotein 1 isoform C
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY031361.1, CF750495.1, AK034817.1 and BE992617.1. On Nov 7, 2013 this sequence version replaced NM_001039352.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (C). ##Evidence-Data-START## Transcript exon combination :: AK129040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAACAGG 
TCCACCAAAG CCCCAAAGGT GAAGTTACAG TCAAATGGAC CAGTGACCAA GAAGGCTAAG 
AAAGAGACTT CATCCAGTGA CAGCAGTGAG GACAGCAGTG AGGACGAGGA CAAAAAAGCC 
CAGGGACTTC CCACACAGAA GGCTGCCGCA CAGGTCAAGC GAGCCAGTGT GCCTCAGCAT 
GCTGGAAAGG CAGCAGCCAA AGCTTCAGAG AGCAGCAGTA GTGAAGAATC CAGTGAGGAA 
GAGGAAGAGG ACAAAAAGAA AAAGCCTGTC CAGCAGAAGG CAGCTAAGCC CCAAGCCAAG 
GCAGTCAGAC CTCCTGCGAA GAAGGCAGAG AGCTCTGAGT CGGACTCAGA CTCGGATTCG 
GACTCCAGCT CAGAGGAAGA AACACCACAG ACCCAGAAGC CAAAGGCAGC TGTGGCAGCA 
AAAGCTCAGA CTAAAGCCGA AGCCAAACCA GGTACACCAG CGAAAGCACA GCCTAAGGTA 
GCCAATGGCA AAGCAGCCGC CAGCAGCAGC AGCAGCAGCA GCAGCGATGA CTCAGAGGAA 
GAGAAGAAGG CAGCTGCACC TCCCAAGAAG ACTGTACCAA AAAAGCAAGT CGTGGCCAAG 
GCCCCAGTGA AAGTAGCTGC CGCCCCCACC CAGAAGAGCT CCAGCAGTGA GGATTCTTCC 
AGTGAAGAGG AGGAGGGACA GAGACAACCC ATGAAGAAAA AAGCAGGTCC CTACAGTTCA 
GTTCCACCAC CCTCTGTTCC TTTACCAAAG AAGTCCCCGG GAACCCAGGC TCCAAAGAAA 
GCTGCTGCGC AGACACAGCC TGCAGACAGC AGTGACGACA GCAGTGACGA TTCTGATTCA 
AGTTCTGAGG AAGAGAAAAA ACCTCCAGCT AAGACGGTCG TCTCCAAGAC ACCCGCCAAA 
GCAGCTCCAG TGAAGAAGAA AGCAGAAAGC TCTTCAGACA GCTCGGATTC TGACAGTTCT 
GAGGATGAAG CTCCTGCCAA GCCAGTCAGT ACAACCAAGA GTCCCAAGCC AGCTGTCACT 
CCGAAGCCAT CTGCAGCAAA GGCAGTGACA ACTCCTAAGC AACCTGCAGG CAGTAACCAG 
AAACCTCAGA GCAGGAAGGC TGACAGCAGC TCCAGCGAGG AGGAAAGCAG CTCCAGCGAG 
GAGGAGGAGG CCTCCAAGAA AAGTGCCACA ACCCCCAAGG CCAAGGTGAC TGCTAAAGCA 
GCACCCGCCA AACAGGCCCC TCAGGCTGCT GGGGACAGCA GCTCTGACTC AGATAGTTCC 
AGCAGTGAAG AGGAGGAGAA GACTCCTAAG CCCCCAGCTA AGAAGAAGGC AGCAGGTGGA 
GCCGTTTCTA CACCAGCCCC TGGGAAGAAA GCAGAGGCCA AGAGCAGCAG CAGCAGCAGC 
AGCAGCAGCT CCGAAGATTC CAGTGAAGAG GAGAAAAAAA AGAAGCCCAA AGCTACTACC 
CCTAAAATAC AGGCAAGCAA GGCCAATGGC ACTCCAGCTT CTCTGAATGG AAAAGCAGCC 
AAGGAAAGTG AGGAGGAAGA GGAGGAGGAA GAAACAGAAG AGAAGAAAAA GGCAGCTGGG 
ACCAAGCCAG GTTCAGGCAA AAAACGGAAG CAGAATGAGA CCGCAGATGA AGCAACAACT 
CCTCAAGCTA AGAAAGTTAA GCTCGAGACC CCCAATACGT TTCCAAAAAG GAAGAAGGGA 
GAAAGAAGGG CGTCTTCCCC TTTCCGAAGG GTCAGGGAGG AGGAGATTGA GGTGGACTCT 
CGAGTGGCGG ACAATTCCTT TGATGCCAAG CGAGGTGCAG CTGGAGACTG GGGGGAGCGA 
GCCAATCAGG TTCTGAAGTT CACCAAAGGC AAGTCCTTCC GGCATGAAAA AACGAAGAAG 
AAGCGAGGCA GCTACCGGGG AGGCTCCATC TCTGTCCAGG TCAATTCCGT CAAATTCGAC 
AGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034441.1
CDS	184..2292
Translation	MADTGLRRVVPSDLYPLVLRFLRDSQLSEVASKFAKATGATQQDANASSLLDIYSFWLNRSTKAPKVKLQSNGPVTKKAKKETSSSDSSEDSSEDEDKKAQGLPTQKAAAQVKRASVPQHAGKAAAKASESSSSEESSEEEEEDKKKKPVQQKAAKPQAKAVRPPAKKAESSESDSDSDSDSSSEEETPQTQKPKAAVAAKAQTKAEAKPGTPAKAQPKVANGKAAASSSSSSSSDDSEEEKKAAAPPKKTVPKKQVVAKAPVKVAAAPTQKSSSSEDSSSEEEEGQRQPMKKKAGPYSSVPPPSVPLPKKSPGTQAPKKAAAQTQPADSSDDSSDDSDSSSEEEKKPPAKTVVSKTPAKAAPVKKKAESSSDSSDSDSSEDEAPAKPVSTTKSPKPAVTPKPSAAKAVTTPKQPAGSNQKPQSRKADSSSSEEESSSSEEEEASKKSATTPKAKVTAKAAPAKQAPQAAGDSSSDSDSSSSEEEEKTPKPPAKKKAAGGAVSTPAPGKKAEAKSSSSSSSSSSEDSSEEEKKKKPKATTPKIQASKANGTPASLNGKAAKESEEEEEEEETEEKKKAAGTKPGSGKKRKQNETADEATTPQAKKVKLETPNTFPKRKKGERRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFDSE

Target ORF information:

RefSeq Version	NM_001039352.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039352.2

ORF Insert Sequence:

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAACAGG 
TCCACCAAAG CCCCAAAGGT GAAGTTACAG TCAAATGGAC CAGTGACCAA GAAGGCTAAG 
AAAGAGACTT CATCCAGTGA CAGCAGTGAG GACAGCAGTG AGGACGAGGA CAAAAAAGCC 
CAGGGACTTC CCACACAGAA GGCTGCCGCA CAGGTCAAGC GAGCCAGTGT GCCTCAGCAT 
GCTGGAAAGG CAGCAGCCAA AGCTTCAGAG AGCAGCAGTA GTGAAGAATC CAGTGAGGAA 
GAGGAAGAGG ACAAAAAGAA AAAGCCTGTC CAGCAGAAGG CAGCTAAGCC CCAAGCCAAG 
GCAGTCAGAC CTCCTGCGAA GAAGGCAGAG AGCTCTGAGT CGGACTCAGA CTCGGATTCG 
GACTCCAGCT CAGAGGAAGA AACACCACAG ACCCAGAAGC CAAAGGCAGC TGTGGCAGCA 
AAAGCTCAGA CTAAAGCCGA AGCCAAACCA GGTACACCAG CGAAAGCACA GCCTAAGGTA 
GCCAATGGCA AAGCAGCCGC CAGCAGCAGC AGCAGCAGCA GCAGCGATGA CTCAGAGGAA 
GAGAAGAAGG CAGCTGCACC TCCCAAGAAG ACTGTACCAA AAAAGCAAGT CGTGGCCAAG 
GCCCCAGTGA AAGTAGCTGC CGCCCCCACC CAGAAGAGCT CCAGCAGTGA GGATTCTTCC 
AGTGAAGAGG AGGAGGGACA GAGACAACCC ATGAAGAAAA AAGCAGGTCC CTACAGTTCA 
GTTCCACCAC CCTCTGTTCC TTTACCAAAG AAGTCCCCGG GAACCCAGGC TCCAAAGAAA 
GCTGCTGCGC AGACACAGCC TGCAGACAGC AGTGACGACA GCAGTGACGA TTCTGATTCA 
AGTTCTGAGG AAGAGAAAAA ACCTCCAGCT AAGACGGTCG TCTCCAAGAC ACCCGCCAAA 
GCAGCTCCAG TGAAGAAGAA AGCAGAAAGC TCTTCAGACA GCTCGGATTC TGACAGTTCT 
GAGGATGAAG CTCCTGCCAA GCCAGTCAGT ACAACCAAGA GTCCCAAGCC AGCTGTCACT 
CCGAAGCCAT CTGCAGCAAA GGCAGTGACA ACTCCTAAGC AACCTGCAGG CAGTAACCAG 
AAACCTCAGA GCAGGAAGGC TGACAGCAGC TCCAGCGAGG AGGAAAGCAG CTCCAGCGAG 
GAGGAGGAGG CCTCCAAGAA AAGTGCCACA ACCCCCAAGG CCAAGGTGAC TGCTAAAGCA 
GCACCCGCCA AACAGGCCCC TCAGGCTGCT GGGGACAGCA GCTCTGACTC AGATAGTTCC 
AGCAGTGAAG AGGAGGAGAA GACTCCTAAG CCCCCAGCTA AGAAGAAGGC AGCAGGTGGA 
GCCGTTTCTA CACCAGCCCC TGGGAAGAAA GCAGAGGCCA AGAGCAGCAG CAGCAGCAGC 
AGCAGCAGCT CCGAAGATTC CAGTGAAGAG GAGAAAAAAA AGAAGCCCAA AGCTACTACC 
CCTAAAATAC AGGCAAGCAA GGCCAATGGC ACTCCAGCTT CTCTGAATGG AAAAGCAGCC 
AAGGAAAGTG AGGAGGAAGA GGAGGAGGAA GAAACAGAAG AGAAGAAAAA GGCAGCTGGG 
ACCAAGCCAG GTTCAGGCAA AAAACGGAAG CAGAATGAGA CCGCAGATGA AGCAACAACT 
CCTCAAGCTA AGAAAGTTAA GCTCGAGACC CCCAATACGT TTCCAAAAAG GAAGAAGGGA 
GAAAGAAGGG CGTCTTCCCC TTTCCGAAGG GTCAGGGAGG AGGAGATTGA GGTGGACTCT 
CGAGTGGCGG ACAATTCCTT TGATGCCAAG CGAGGTGCAG CTGGAGACTG GGGGGAGCGA 
GCCAATCAGG TTCTGAAGTT CACCAAAGGC AAGTCCTTCC GGCATGAAAA AACGAAGAAG 
AAGCGAGGCA GCTACCGGGG AGGCTCCATC TCTGTCCAGG TCAATTCCGT CAAATTCGAC 
AGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nolc1 ( NM_001039353.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034442.1 Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu00595
Clone ID Related Accession (Same CDS sequence)	NM_001039353.2
Accession Version	NM_001039353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	nucleolar and coiled-body phosphoprotein 1 isoform D
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY031361.1, AK147118.1, AK034817.1 and BE992617.1. On Nov 7, 2013 this sequence version replaced NM_001039353.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the central coding region, compared to variant 3. The encoded isoform (D) is shorter, compared to isoform C. ##Evidence-Data-START## Transcript exon combination :: AK147118.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAAGTCC 
ACCAAAGCCC CAAAGGTGAA GTTACAGTCA AATGGACCAG TGACCAAGAA GGCTAAGAAA 
GAGACTTCAT CCAGTGACAG CAGTGAGGAC AGCAGTGAGG ACGAGGACAA AAAAGCCCAG 
GGACTTCCCA CACAGAAGGC TGCCGCACAG GTCAAGCGAG CCAGTGTGCC TCAGCATGCT 
GGAAAGGCAG CAGCCAAAGC TTCAGAGAGC AGCAGTAGTG AAGAATCCAG TGAGGAAGAG 
GAAGAGGACA AAAAGAAAAA GCCTGTCCAG CAGAAGGCAG CTAAGCCCCA AGCCAAGGCA 
GTCAGACCTC CTGCGAAGAA GGCAGAGAGC TCTGAGTCGG ACTCAGACTC GGATTCGGAC 
TCCAGCTCAG AGGAAGAAAC ACCACAGACC CAGAAGCCAA AGGCAGCTGT GGCAGCAAAA 
GCTCAGACTA AAGCCGAAGC CAAACCAGGT ACACCAGCGA AAGCACAGCC TAAGGTAGCC 
AATGGCAAAG CAGCCGCCAG CAGCAGCAGC AGCAGCAGCA GCGATGACTC AGAGGAAGAG 
AAGAAGGCAG CTGCACCTCC CAAGAAGACT GTACCAAAAA AGCAAGTCGT GGCCAAGGCC 
CCAGTGAAAG TAGCTGCCGC CCCCACCCAG AAGAGCTCCA GCAGTGAGGA TTCTTCCAGT 
GAAGAGGAGG AGGGACAGAG ACAACCCATG AAGAAAAAAG CAGGTCCCTA CAGTTCAGTT 
CCACCACCCT CTGTTCCTTT ACCAAAGAAG TCCCCGGGAA CCCAGGCTCC AAAGAAAGCT 
GCTGCGCAGA CACAGCCTGC AGACAGCAGT GACGACAGCA GTGACGATTC TGATTCAAGT 
TCTGAGGAAG AGAAAAAACC TCCAGCTAAG ACGGTCGTCT CCAAGACACC CGCCAAAGCA 
GCTCCAGTGA AGAAGAAAGC AGAAAGCTCT TCAGACAGCT CGGATTCTGA CAGTTCTGAG 
GATGAAGCTC CTGCCAAGCC AGTCAGTACA ACCAAGAGTC CCAAGCCAGC TGTCACTCCG 
AAGCCATCTG CAGCAAAGGC AGTGACAACT CCTAAGCAAC CTGCAGGCAG TAACCAGAAA 
CCTCAGAGCA GGAAGGCTGA CAGCAGCTCC AGCGAGGAGG AAAGCAGCTC CAGCGAGGAG 
GAGGAGGCCT CCAAGAAAAG TGCCACAACC CCCAAGGCCA AGGTGACTGC TAAAGCAGCA 
CCCGCCAAAC AGGCCCCTCA GGCTGCTGGG GACAGCAGCT CTGACTCAGA TAGTTCCAGC 
AGTGAAGAGG AGGAGAAGAC TCCTAAGCCC CCAGCTAAGA AGAAGGCAGC AGGTGGAGCC 
GTTTCTACAC CAGCCCCTGG GAAGAAAGCA GAGGCCAAGA GCAGCAGCAG CAGCAGCAGC 
AGCAGCTCCG AAGATTCCAG TGAAGAGGAG AAAAAAAAGA AGCCCAAAGC TACTACCCCT 
AAAATACAGG CAAGCAAGGC CAATGGCACT CCAGCTTCTC TGAATGGAAA AGCAGCCAAG 
GAAAGTGAGG AGGAAGAGGA GGAGGAAGAA ACAGAAGAGA AGAAAAAGGC AGCTGGGACC 
AAGCCAGGTT CAGGCAAAAA ACGGAAGCAG AATGAGACCG CAGATGAAGC AACAACTCCT 
CAAGCTAAGA AAGTTAAGCT CGAGACCCCC AATACGTTTC CAAAAAGGAA GAAGGGAGAA 
AGAAGGGCGT CTTCCCCTTT CCGAAGGGTC AGGGAGGAGG AGATTGAGGT GGACTCTCGA 
GTGGCGGACA ATTCCTTTGA TGCCAAGCGA GGTGCAGCTG GAGACTGGGG GGAGCGAGCC 
AATCAGGTTC TGAAGTTCAC CAAAGGCAAG TCCTTCCGGC ATGAAAAAAC GAAGAAGAAG 
CGAGGCAGCT ACCGGGGAGG CTCCATCTCT GTCCAGGTCA ATTCCGTCAA ATTCGACAGC 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034442.1
CDS	184..2289
Translation	MADTGLRRVVPSDLYPLVLRFLRDSQLSEVASKFAKATGATQQDANASSLLDIYSFWLKSTKAPKVKLQSNGPVTKKAKKETSSSDSSEDSSEDEDKKAQGLPTQKAAAQVKRASVPQHAGKAAAKASESSSSEESSEEEEEDKKKKPVQQKAAKPQAKAVRPPAKKAESSESDSDSDSDSSSEEETPQTQKPKAAVAAKAQTKAEAKPGTPAKAQPKVANGKAAASSSSSSSSDDSEEEKKAAAPPKKTVPKKQVVAKAPVKVAAAPTQKSSSSEDSSSEEEEGQRQPMKKKAGPYSSVPPPSVPLPKKSPGTQAPKKAAAQTQPADSSDDSSDDSDSSSEEEKKPPAKTVVSKTPAKAAPVKKKAESSSDSSDSDSSEDEAPAKPVSTTKSPKPAVTPKPSAAKAVTTPKQPAGSNQKPQSRKADSSSSEEESSSSEEEEASKKSATTPKAKVTAKAAPAKQAPQAAGDSSSDSDSSSSEEEEKTPKPPAKKKAAGGAVSTPAPGKKAEAKSSSSSSSSSSEDSSEEEKKKKPKATTPKIQASKANGTPASLNGKAAKESEEEEEEEETEEKKKAAGTKPGSGKKRKQNETADEATTPQAKKVKLETPNTFPKRKKGERRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFDSE

Target ORF information:

RefSeq Version	NM_001039353.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039353.2

ORF Insert Sequence:

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAAGTCC 
ACCAAAGCCC CAAAGGTGAA GTTACAGTCA AATGGACCAG TGACCAAGAA GGCTAAGAAA 
GAGACTTCAT CCAGTGACAG CAGTGAGGAC AGCAGTGAGG ACGAGGACAA AAAAGCCCAG 
GGACTTCCCA CACAGAAGGC TGCCGCACAG GTCAAGCGAG CCAGTGTGCC TCAGCATGCT 
GGAAAGGCAG CAGCCAAAGC TTCAGAGAGC AGCAGTAGTG AAGAATCCAG TGAGGAAGAG 
GAAGAGGACA AAAAGAAAAA GCCTGTCCAG CAGAAGGCAG CTAAGCCCCA AGCCAAGGCA 
GTCAGACCTC CTGCGAAGAA GGCAGAGAGC TCTGAGTCGG ACTCAGACTC GGATTCGGAC 
TCCAGCTCAG AGGAAGAAAC ACCACAGACC CAGAAGCCAA AGGCAGCTGT GGCAGCAAAA 
GCTCAGACTA AAGCCGAAGC CAAACCAGGT ACACCAGCGA AAGCACAGCC TAAGGTAGCC 
AATGGCAAAG CAGCCGCCAG CAGCAGCAGC AGCAGCAGCA GCGATGACTC AGAGGAAGAG 
AAGAAGGCAG CTGCACCTCC CAAGAAGACT GTACCAAAAA AGCAAGTCGT GGCCAAGGCC 
CCAGTGAAAG TAGCTGCCGC CCCCACCCAG AAGAGCTCCA GCAGTGAGGA TTCTTCCAGT 
GAAGAGGAGG AGGGACAGAG ACAACCCATG AAGAAAAAAG CAGGTCCCTA CAGTTCAGTT 
CCACCACCCT CTGTTCCTTT ACCAAAGAAG TCCCCGGGAA CCCAGGCTCC AAAGAAAGCT 
GCTGCGCAGA CACAGCCTGC AGACAGCAGT GACGACAGCA GTGACGATTC TGATTCAAGT 
TCTGAGGAAG AGAAAAAACC TCCAGCTAAG ACGGTCGTCT CCAAGACACC CGCCAAAGCA 
GCTCCAGTGA AGAAGAAAGC AGAAAGCTCT TCAGACAGCT CGGATTCTGA CAGTTCTGAG 
GATGAAGCTC CTGCCAAGCC AGTCAGTACA ACCAAGAGTC CCAAGCCAGC TGTCACTCCG 
AAGCCATCTG CAGCAAAGGC AGTGACAACT CCTAAGCAAC CTGCAGGCAG TAACCAGAAA 
CCTCAGAGCA GGAAGGCTGA CAGCAGCTCC AGCGAGGAGG AAAGCAGCTC CAGCGAGGAG 
GAGGAGGCCT CCAAGAAAAG TGCCACAACC CCCAAGGCCA AGGTGACTGC TAAAGCAGCA 
CCCGCCAAAC AGGCCCCTCA GGCTGCTGGG GACAGCAGCT CTGACTCAGA TAGTTCCAGC 
AGTGAAGAGG AGGAGAAGAC TCCTAAGCCC CCAGCTAAGA AGAAGGCAGC AGGTGGAGCC 
GTTTCTACAC CAGCCCCTGG GAAGAAAGCA GAGGCCAAGA GCAGCAGCAG CAGCAGCAGC 
AGCAGCTCCG AAGATTCCAG TGAAGAGGAG AAAAAAAAGA AGCCCAAAGC TACTACCCCT 
AAAATACAGG CAAGCAAGGC CAATGGCACT CCAGCTTCTC TGAATGGAAA AGCAGCCAAG 
GAAAGTGAGG AGGAAGAGGA GGAGGAAGAA ACAGAAGAGA AGAAAAAGGC AGCTGGGACC 
AAGCCAGGTT CAGGCAAAAA ACGGAAGCAG AATGAGACCG CAGATGAAGC AACAACTCCT 
CAAGCTAAGA AAGTTAAGCT CGAGACCCCC AATACGTTTC CAAAAAGGAA GAAGGGAGAA 
AGAAGGGCGT CTTCCCCTTT CCGAAGGGTC AGGGAGGAGG AGATTGAGGT GGACTCTCGA 
GTGGCGGACA ATTCCTTTGA TGCCAAGCGA GGTGCAGCTG GAGACTGGGG GGAGCGAGCC 
AATCAGGTTC TGAAGTTCAC CAAAGGCAAG TCCTTCCGGC ATGAAAAAAC GAAGAAGAAG 
CGAGGCAGCT ACCGGGGAGG CTCCATCTCT GTCCAGGTCA ATTCCGTCAA ATTCGACAGC 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nolc1 ( NM_053086.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444316.2 Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 1, mRNA.

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CloneID	OMu09048
Clone ID Related Accession (Same CDS sequence)	NM_053086.3
Accession Version	NM_053086.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	nucleolar and coiled-body phosphoprotein 1 isoform A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY031361.1, AK166753.1, AK147118.1 and BE992617.1. On Nov 7, 2013 this sequence version replaced NM_053086.2. Transcript Variant: This variant (1) uses an alternate in-frame splice site in the central coding region, compared to variant 3. The encoded isoform (A) is shorter, compared to isoform C. ##Evidence-Data-START## Transcript exon combination :: AK166753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAACAGG 
TCCACCAAAG CCCCAAAGGT GAAGTTACAG TCAAATGGAC CAGTGACCAA GAAGGCTAAG 
AAAGAGACTT CATCCAGTGA CAGCAGTGAG GACAGCAGTG AGGACGAGGA CAAAAAAGCC 
CAGGGACTTC CCACACAGAA GGCTGCCGCA CAGGTCAAGC GAGCCAGTGT GCCTCAGCAT 
GCTGGAAAGG CAGCAGCCAA AGCTTCAGAG AGCAGCAGTA GTGAAGAATC CAGTGAGGAA 
GAGGAAGAGG ACAAAAAGAA AAAGCCTGTC CAGAAGGCAG CTAAGCCCCA AGCCAAGGCA 
GTCAGACCTC CTGCGAAGAA GGCAGAGAGC TCTGAGTCGG ACTCAGACTC GGATTCGGAC 
TCCAGCTCAG AGGAAGAAAC ACCACAGACC CAGAAGCCAA AGGCAGCTGT GGCAGCAAAA 
GCTCAGACTA AAGCCGAAGC CAAACCAGGT ACACCAGCGA AAGCACAGCC TAAGGTAGCC 
AATGGCAAAG CAGCCGCCAG CAGCAGCAGC AGCAGCAGCA GCGATGACTC AGAGGAAGAG 
AAGAAGGCAG CTGCACCTCC CAAGAAGACT GTACCAAAAA AGCAAGTCGT GGCCAAGGCC 
CCAGTGAAAG TAGCTGCCGC CCCCACCCAG AAGAGCTCCA GCAGTGAGGA TTCTTCCAGT 
GAAGAGGAGG AGGGACAGAG ACAACCCATG AAGAAAAAAG CAGGTCCCTA CAGTTCAGTT 
CCACCACCCT CTGTTCCTTT ACCAAAGAAG TCCCCGGGAA CCCAGGCTCC AAAGAAAGCT 
GCTGCGCAGA CACAGCCTGC AGACAGCAGT GACGACAGCA GTGACGATTC TGATTCAAGT 
TCTGAGGAAG AGAAAAAACC TCCAGCTAAG ACGGTCGTCT CCAAGACACC CGCCAAAGCA 
GCTCCAGTGA AGAAGAAAGC AGAAAGCTCT TCAGACAGCT CGGATTCTGA CAGTTCTGAG 
GATGAAGCTC CTGCCAAGCC AGTCAGTACA ACCAAGAGTC CCAAGCCAGC TGTCACTCCG 
AAGCCATCTG CAGCAAAGGC AGTGACAACT CCTAAGCAAC CTGCAGGCAG TAACCAGAAA 
CCTCAGAGCA GGAAGGCTGA CAGCAGCTCC AGCGAGGAGG AAAGCAGCTC CAGCGAGGAG 
GAGGAGGCCT CCAAGAAAAG TGCCACAACC CCCAAGGCCA AGGTGACTGC TAAAGCAGCA 
CCCGCCAAAC AGGCCCCTCA GGCTGCTGGG GACAGCAGCT CTGACTCAGA TAGTTCCAGC 
AGTGAAGAGG AGGAGAAGAC TCCTAAGCCC CCAGCTAAGA AGAAGGCAGC AGGTGGAGCC 
GTTTCTACAC CAGCCCCTGG GAAGAAAGCA GAGGCCAAGA GCAGCAGCAG CAGCAGCAGC 
AGCAGCTCCG AAGATTCCAG TGAAGAGGAG AAAAAAAAGA AGCCCAAAGC TACTACCCCT 
AAAATACAGG CAAGCAAGGC CAATGGCACT CCAGCTTCTC TGAATGGAAA AGCAGCCAAG 
GAAAGTGAGG AGGAAGAGGA GGAGGAAGAA ACAGAAGAGA AGAAAAAGGC AGCTGGGACC 
AAGCCAGGTT CAGGCAAAAA ACGGAAGCAG AATGAGACCG CAGATGAAGC AACAACTCCT 
CAAGCTAAGA AAGTTAAGCT CGAGACCCCC AATACGTTTC CAAAAAGGAA GAAGGGAGAA 
AGAAGGGCGT CTTCCCCTTT CCGAAGGGTC AGGGAGGAGG AGATTGAGGT GGACTCTCGA 
GTGGCGGACA ATTCCTTTGA TGCCAAGCGA GGTGCAGCTG GAGACTGGGG GGAGCGAGCC 
AATCAGGTTC TGAAGTTCAC CAAAGGCAAG TCCTTCCGGC ATGAAAAAAC GAAGAAGAAG 
CGAGGCAGCT ACCGGGGAGG CTCCATCTCT GTCCAGGTCA ATTCCGTCAA ATTCGACAGC 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444316.2
CDS	184..2289
Translation	MADTGLRRVVPSDLYPLVLRFLRDSQLSEVASKFAKATGATQQDANASSLLDIYSFWLNRSTKAPKVKLQSNGPVTKKAKKETSSSDSSEDSSEDEDKKAQGLPTQKAAAQVKRASVPQHAGKAAAKASESSSSEESSEEEEEDKKKKPVQKAAKPQAKAVRPPAKKAESSESDSDSDSDSSSEEETPQTQKPKAAVAAKAQTKAEAKPGTPAKAQPKVANGKAAASSSSSSSSDDSEEEKKAAAPPKKTVPKKQVVAKAPVKVAAAPTQKSSSSEDSSSEEEEGQRQPMKKKAGPYSSVPPPSVPLPKKSPGTQAPKKAAAQTQPADSSDDSSDDSDSSSEEEKKPPAKTVVSKTPAKAAPVKKKAESSSDSSDSDSSEDEAPAKPVSTTKSPKPAVTPKPSAAKAVTTPKQPAGSNQKPQSRKADSSSSEEESSSSEEEEASKKSATTPKAKVTAKAAPAKQAPQAAGDSSSDSDSSSSEEEEKTPKPPAKKKAAGGAVSTPAPGKKAEAKSSSSSSSSSSEDSSEEEKKKKPKATTPKIQASKANGTPASLNGKAAKESEEEEEEEETEEKKKAAGTKPGSGKKRKQNETADEATTPQAKKVKLETPNTFPKRKKGERRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFDSE

Target ORF information:

RefSeq Version	NM_053086.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053086.3

ORF Insert Sequence:

ATGGCGGATA CCGGCTTGCG CCGCGTGGTT CCCAGCGACC TTTATCCCCT TGTGCTCAGA 
TTTCTGCGGG ATAGCCAACT CTCGGAGGTG GCCAGTAAAT TTGCAAAAGC GACCGGCGCT 
ACACAGCAGG ACGCCAATGC CTCGTCCCTC TTGGACATCT ATAGCTTCTG GCTCAACAGG 
TCCACCAAAG CCCCAAAGGT GAAGTTACAG TCAAATGGAC CAGTGACCAA GAAGGCTAAG 
AAAGAGACTT CATCCAGTGA CAGCAGTGAG GACAGCAGTG AGGACGAGGA CAAAAAAGCC 
CAGGGACTTC CCACACAGAA GGCTGCCGCA CAGGTCAAGC GAGCCAGTGT GCCTCAGCAT 
GCTGGAAAGG CAGCAGCCAA AGCTTCAGAG AGCAGCAGTA GTGAAGAATC CAGTGAGGAA 
GAGGAAGAGG ACAAAAAGAA AAAGCCTGTC CAGAAGGCAG CTAAGCCCCA AGCCAAGGCA 
GTCAGACCTC CTGCGAAGAA GGCAGAGAGC TCTGAGTCGG ACTCAGACTC GGATTCGGAC 
TCCAGCTCAG AGGAAGAAAC ACCACAGACC CAGAAGCCAA AGGCAGCTGT GGCAGCAAAA 
GCTCAGACTA AAGCCGAAGC CAAACCAGGT ACACCAGCGA AAGCACAGCC TAAGGTAGCC 
AATGGCAAAG CAGCCGCCAG CAGCAGCAGC AGCAGCAGCA GCGATGACTC AGAGGAAGAG 
AAGAAGGCAG CTGCACCTCC CAAGAAGACT GTACCAAAAA AGCAAGTCGT GGCCAAGGCC 
CCAGTGAAAG TAGCTGCCGC CCCCACCCAG AAGAGCTCCA GCAGTGAGGA TTCTTCCAGT 
GAAGAGGAGG AGGGACAGAG ACAACCCATG AAGAAAAAAG CAGGTCCCTA CAGTTCAGTT 
CCACCACCCT CTGTTCCTTT ACCAAAGAAG TCCCCGGGAA CCCAGGCTCC AAAGAAAGCT 
GCTGCGCAGA CACAGCCTGC AGACAGCAGT GACGACAGCA GTGACGATTC TGATTCAAGT 
TCTGAGGAAG AGAAAAAACC TCCAGCTAAG ACGGTCGTCT CCAAGACACC CGCCAAAGCA 
GCTCCAGTGA AGAAGAAAGC AGAAAGCTCT TCAGACAGCT CGGATTCTGA CAGTTCTGAG 
GATGAAGCTC CTGCCAAGCC AGTCAGTACA ACCAAGAGTC CCAAGCCAGC TGTCACTCCG 
AAGCCATCTG CAGCAAAGGC AGTGACAACT CCTAAGCAAC CTGCAGGCAG TAACCAGAAA 
CCTCAGAGCA GGAAGGCTGA CAGCAGCTCC AGCGAGGAGG AAAGCAGCTC CAGCGAGGAG 
GAGGAGGCCT CCAAGAAAAG TGCCACAACC CCCAAGGCCA AGGTGACTGC TAAAGCAGCA 
CCCGCCAAAC AGGCCCCTCA GGCTGCTGGG GACAGCAGCT CTGACTCAGA TAGTTCCAGC 
AGTGAAGAGG AGGAGAAGAC TCCTAAGCCC CCAGCTAAGA AGAAGGCAGC AGGTGGAGCC 
GTTTCTACAC CAGCCCCTGG GAAGAAAGCA GAGGCCAAGA GCAGCAGCAG CAGCAGCAGC 
AGCAGCTCCG AAGATTCCAG TGAAGAGGAG AAAAAAAAGA AGCCCAAAGC TACTACCCCT 
AAAATACAGG CAAGCAAGGC CAATGGCACT CCAGCTTCTC TGAATGGAAA AGCAGCCAAG 
GAAAGTGAGG AGGAAGAGGA GGAGGAAGAA ACAGAAGAGA AGAAAAAGGC AGCTGGGACC 
AAGCCAGGTT CAGGCAAAAA ACGGAAGCAG AATGAGACCG CAGATGAAGC AACAACTCCT 
CAAGCTAAGA AAGTTAAGCT CGAGACCCCC AATACGTTTC CAAAAAGGAA GAAGGGAGAA 
AGAAGGGCGT CTTCCCCTTT CCGAAGGGTC AGGGAGGAGG AGATTGAGGT GGACTCTCGA 
GTGGCGGACA ATTCCTTTGA TGCCAAGCGA GGTGCAGCTG GAGACTGGGG GGAGCGAGCC 
AATCAGGTTC TGAAGTTCAC CAAAGGCAAG TCCTTCCGGC ATGAAAAAAC GAAGAAGAAG 
CGAGGCAGCT ACCGGGGAGG CTCCATCTCT GTCCAGGTCA ATTCCGTCAA ATTCGACAGC 
GAGTGA