Usp46 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp46
Entrez Gene ID	69727
Full Name	ubiquitin specific peptidase 46
Synonyms	1190009E20Rik,2410018I08Rik,AI451644
General protein information	Preferred Names ubiquitin specific peptidase 46 Names ubiquitin carboxyl-terminal hydrolase 46 deubiquitinating enzyme 46 ubiquitin specific protease 46 ubiquitin thioesterase 46 ubiquitin thiolesterase 46 ubiquitin-specific-processing protease 46
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006504268.3	XP_006504331.1	ubiquitin carboxyl-terminal hydrolase 46 isoform X1
NM_177561.3	NP_808229.1	ubiquitin carboxyl-terminal hydrolase 46
XM_006504268.4	XP_006504331.1	ubiquitin carboxyl-terminal hydrolase 46 isoform X1
XM_006504269.2	XP_006504332.1	ubiquitin carboxyl-terminal hydrolase 46 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Usp46	NP_001178525.1
Drosophila melanogaster (fruit fly)	CG7023	NP_651099.1
Saccharomyces cerevisiae (baker's yeast)	UBP13	NP_009486.3
Eremothecium gossypii	AGOS_AGR370W	NP_987036.2
Bos taurus (cattle)	USP46	NP_001179373.2
Gallus gallus (chicken)	USP46	XP_420711.1
Danio rerio (zebrafish)	usp46	NP_001231910.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012139	XP_320388.3
Xenopus tropicalis (tropical clawed frog)	usp46	NP_001106637.1
Homo sapiens (human)	USP46	NP_073743.2
	USP46	XP_001092352.1
Mus musculus (house mouse)	Usp46	NP_808229.1
Arabidopsis thaliana (thale cress)	UBP4	NP_565532.1
Oryza sativa (rice)	Os04g0452400	NP_001052951.1
Pan troglodytes (chimpanzee)	USP46	XP_517296.2
Canis lupus familiaris (dog)	USP46	XP_005628243.1
Caenorhabditis elegans (roundworm)	usp-46	NP_499162.2
Saccharomyces cerevisiae (baker's yeast)	UBP9	NP_011024.1
Kluyveromyces lactis	KLLA0E20351g	XP_454871.1
Arabidopsis thaliana (thale cress)	UBP3	NP_568074.1

Gene Ontology
Bibliography

Related articles in PubMed

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Jahan AS, Lestra M, Swee LK, Fan Y, Lamers MM, Tafesse FG, Theile CS, Spooner E, Bruzzone R, Ploegh HL, Sanyal S
Proceedings of the National Academy of Sciences of the United States of America113(6)E705-14(2016 Feb)

Impaired Maternal Behavior in Usp46 Mutant Mice: A Model for Trans-Generational Transmission of Maternal Care.
Umemura S, Imai S, Mimura A, Fujiwara M, Ebihara S
PloS one10(8)e0136016(2015)

Behavioral characteristics of ubiquitin-specific peptidase 46-deficient mice.
Imai S, Kano M, Nonoyama K, Ebihara S
PloS one8(3)e58566(2013)

Ubiquitin-specific peptidase 46 (Usp46) regulates mouse immobile behavior in the tail suspension test through the GABAergic system.
Imai S, Mamiya T, Tsukada A, Sakai Y, Mouri A, Nabeshima T, Ebihara S
PloS one7(6)e39084(2012)

Lysine 92 amino acid residue of USP46, a gene associated with 'behavioral despair' in mice, influences the deubiquitinating enzyme activity.
Zhang W, Tian QB, Li QK, Wang JM, Wang CN, Liu T, Liu DW, Wang MW
PloS one6(10)e26297(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Usp46 mutation is associated with Impaired Maternal Behavior.
Title: Impaired Maternal Behavior in Usp46 Mutant Mice: A Model for Trans-Generational Transmission of Maternal Care.

Usp46 regulates a wide range of behavioral phenotypes that might be related to mental disorders
Title: Behavioral characteristics of ubiquitin-specific peptidase 46-deficient mice.

results suggest that the Lys 92 deletion of USP46 could influence enzyme activity and thereby provide a molecular clue how the enzyme regulating the pathogenesis of mental illnesses.
Title: Lysine 92 amino acid residue of USP46, a gene associated with 'behavioral despair' in mice, influences the deubiquitinating enzyme activity.

The following Usp46 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp46 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu35308	XM_006504269.2 Latest version!	Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu06437	NM_177561.3 Latest version!	Mus musculus ubiquitin specific peptidase 46 (Usp46), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu35307	XM_006504268.4 Latest version!	Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu35307	XM_006504268.3	Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp46 ( XM_006504269.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504332.1 Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu35308
Clone ID Related Accession (Same CDS sequence)	XM_006504269.2
Accession Version	XM_006504269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 46 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006504269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGG ATGCTCACGA GTTCTTAAAT TATTTGCTAA ACACTATTGC GGACATCCTG 
CAGGAAGAGA AGAAACAGGA AAAACAAAAC GGGAAGTTAA AAAACGGCAA CATGAACGAA 
CCAGCCGAGA ACAATAAGCC AGAACTCACC TGGGTCCACG AGATTTTTCA GGGAACACTC 
ACTAACGAAA CTCGATGCTT GAACTGTGAA ACTGTGAGTA GCAAAGATGA AGATTTTCTT 
GACCTCTCTG TCGATGTGGA ACAGAACACC TCCATCACCC ACTGTCTGAG AGACTTCAGC 
AACACAGAAA CGCTGTGTAG TGAACAAAAA TATTATTGTG AAACATGTTG CAGCAAACAG 
GAGGCTCAGA AGAGGATGAG GGTAAAAAAG CTGCCCATGA TTTTGGCCCT GCACCTCAAG 
AGGTTCAAGT ACATGGAACA GCTCCACAGG TACACCAAGC TGTCTTACCG GGTGGTCTTC 
CCTCTGGAAC TCCGGCTCTT CAACACCTCC AGCGATGCTG TGAACCTGGA CCGCATGTAT 
GACCTGGTTG CTGTGGTCGT TCACTGTGGA AGTGGTCCTA ACCGTGGGCA TTACATCACC 
ATCGTAAAAA GTCATGGCTT CTGGCTGTTG TTTGATGACG ACATTGTAGA GAAAATAGAC 
GCTCAAGCCA TTGAGGAATT CTATGGTCTA ACGTCAGATA TATCAAAAAA TTCAGAATCT 
GGATATATTT TATTCTATCA ATCAAGAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504332.1
CDS	321..1073
Translation	MQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

Target ORF information:

RefSeq Version	XM_006504269.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504269.2

ORF Insert Sequence:

ATGCAGCAGG ATGCTCACGA GTTCTTAAAT TATTTGCTAA ACACTATTGC GGACATCCTG 
CAGGAAGAGA AGAAACAGGA AAAACAAAAC GGGAAGTTAA AAAACGGCAA CATGAACGAA 
CCAGCCGAGA ACAATAAGCC AGAACTCACC TGGGTCCACG AGATTTTTCA GGGAACACTC 
ACTAACGAAA CTCGATGCTT GAACTGTGAA ACTGTGAGTA GCAAAGATGA AGATTTTCTT 
GACCTCTCTG TCGATGTGGA ACAGAACACC TCCATCACCC ACTGTCTGAG AGACTTCAGC 
AACACAGAAA CGCTGTGTAG TGAACAAAAA TATTATTGTG AAACATGTTG CAGCAAACAG 
GAGGCTCAGA AGAGGATGAG GGTAAAAAAG CTGCCCATGA TTTTGGCCCT GCACCTCAAG 
AGGTTCAAGT ACATGGAACA GCTCCACAGG TACACCAAGC TGTCTTACCG GGTGGTCTTC 
CCTCTGGAAC TCCGGCTCTT CAACACCTCC AGCGATGCTG TGAACCTGGA CCGCATGTAT 
GACCTGGTTG CTGTGGTCGT TCACTGTGGA AGTGGTCCTA ACCGTGGGCA TTACATCACC 
ATCGTAAAAA GTCATGGCTT CTGGCTGTTG TTTGATGACG ACATTGTAGA GAAAATAGAC 
GCTCAAGCCA TTGAGGAATT CTATGGTCTA ACGTCAGATA TATCAAAAAA TTCAGAATCT 
GGATATATTT TATTCTATCA ATCAAGAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp46 ( XM_006504268.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504331.1 Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35307
Clone ID Related Accession (Same CDS sequence)	XM_006504268.3 , XM_006504268.4
Accession Version	XM_006504268.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 46 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTGTT TCCAGGGCAC CAATGCCTCT GCTCTGGAAA AAGACATTGG ACCAGAGCAG 
TTTCCGATCA ATGAACACTA CTTCGGATTG GTCAATTTTG GAAACACATG CTACTGTAAC 
TCCGTGCTTC AAGCGCTGTA CTTCTGCCGG CCGTTCCGGG AGAATGTGTT GGCATACAAG 
GCCCAGCAAA AAAAGAAGGA AAACCTGTTG ACATGCCTGG CAGACCTTTT CCACAGCATT 
GCCACGCAGA AGAAGAAGGT TGGCGTCATC CCACCAAAGA AGTTCATTTC AAGGCTGAGG 
AAAGAGAACG ATCTCTTTGA TAACTACATG CAGCAGGATG CTCACGAGTT CTTAAATTAT 
TTGCTAAACA CTATTGCGGA CATCCTGCAG GAAGAGAAGA AACAGGAAAA ACAAAACGGG 
AAGTTAAAAA ACGGCAACAT GAACGAACCA GCCGAGAACA ATAAGCCAGA ACTCACCTGG 
GTCCACGAGA TTTTTCAGGG AACACTCACT AACGAAACTC GATGCTTGAA CTGTGAAACT 
GTGAGTAGCA AAGATGAAGA TTTTCTTGAC CTCTCTGTCG ATGTGGAACA GAACACCTCC 
ATCACCCACT GTCTGAGAGA CTTCAGCAAC ACAGAAACGC TGTGTAGTGA ACAAAAATAT 
TATTGTGAAA CATGTTGCAG CAAACAGGAG GCTCAGAAGA GGATGAGGGT AAAAAAGCTG 
CCCATGATTT TGGCCCTGCA CCTCAAGAGG TTCAAGTACA TGGAACAGCT CCACAGGTAC 
ACCAAGCTGT CTTACCGGGT GGTCTTCCCT CTGGAACTCC GGCTCTTCAA CACCTCCAGC 
GATGCTGTGA ACCTGGACCG CATGTATGAC CTGGTTGCTG TGGTCGTTCA CTGTGGAAGT 
GGTCCTAACC GTGGGCATTA CATCACCATC GTAAAAAGTC ATGGCTTCTG GCTGTTGTTT 
GATGACGACA TTGTAGAGAA AATAGACGCT CAAGCCATTG AGGAATTCTA TGGTCTAACG 
TCAGATATAT CAAAAAATTC AGAATCTGGA TATATTTTAT TCTATCAATC AAGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504331.1
CDS	1439..2518
Translation	MNCFQGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

Target ORF information:

RefSeq Version	XM_006504268.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504268.3

ORF Insert Sequence:

ATGAACTGTT TCCAGGGCAC CAATGCCTCT GCTCTGGAAA AAGACATTGG ACCAGAGCAG 
TTTCCGATCA ATGAACACTA CTTCGGATTG GTCAATTTTG GAAACACATG CTACTGTAAC 
TCCGTGCTTC AAGCGCTGTA CTTCTGCCGG CCGTTCCGGG AGAATGTGTT GGCATACAAG 
GCCCAGCAAA AAAAGAAGGA AAACCTGTTG ACATGCCTGG CAGACCTTTT CCACAGCATT 
GCCACGCAGA AGAAGAAGGT TGGCGTCATC CCACCAAAGA AGTTCATTTC AAGGCTGAGG 
AAAGAGAACG ATCTCTTTGA TAACTACATG CAGCAGGATG CTCACGAGTT CTTAAATTAT 
TTGCTAAACA CTATTGCGGA CATCCTGCAG GAAGAGAAGA AACAGGAAAA ACAAAACGGG 
AAGTTAAAAA ACGGCAACAT GAACGAACCA GCCGAGAACA ATAAGCCAGA ACTCACCTGG 
GTCCACGAGA TTTTTCAGGG AACACTCACT AACGAAACTC GATGCTTGAA CTGTGAAACT 
GTGAGTAGCA AAGATGAAGA TTTTCTTGAC CTCTCTGTCG ATGTGGAACA GAACACCTCC 
ATCACCCACT GTCTGAGAGA CTTCAGCAAC ACAGAAACGC TGTGTAGTGA ACAAAAATAT 
TATTGTGAAA CATGTTGCAG CAAACAGGAG GCTCAGAAGA GGATGAGGGT AAAAAAGCTG 
CCCATGATTT TGGCCCTGCA CCTCAAGAGG TTCAAGTACA TGGAACAGCT CCACAGGTAC 
ACCAAGCTGT CTTACCGGGT GGTCTTCCCT CTGGAACTCC GGCTCTTCAA CACCTCCAGC 
GATGCTGTGA ACCTGGACCG CATGTATGAC CTGGTTGCTG TGGTCGTTCA CTGTGGAAGT 
GGTCCTAACC GTGGGCATTA CATCACCATC GTAAAAAGTC ATGGCTTCTG GCTGTTGTTT 
GATGACGACA TTGTAGAGAA AATAGACGCT CAAGCCATTG AGGAATTCTA TGGTCTAACG 
TCAGATATAT CAAAAAATTC AGAATCTGGA TATATTTTAT TCTATCAATC AAGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp46 ( NM_177561.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808229.1 Mus musculus ubiquitin specific peptidase 46 (Usp46), mRNA.

Price & Availability↑

CloneID	OMu06437
Clone ID Related Accession (Same CDS sequence)	NM_177561.3
Accession Version	NM_177561.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 46
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC164570.3 and AC242530.3. On Nov 17, 2006 this sequence version replaced NM_177561.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC039916.1, SRR1660815.85407.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGACTGTCC GAAACATCGC CTCCATCTGT AATATGGGCA CCAATGCCTC TGCTCTGGAA 
AAAGACATTG GACCAGAGCA GTTTCCGATC AATGAACACT ACTTCGGATT GGTCAATTTT 
GGAAACACAT GCTACTGTAA CTCCGTGCTT CAAGCGCTGT ACTTCTGCCG GCCGTTCCGG 
GAGAATGTGT TGGCATACAA GGCCCAGCAA AAAAAGAAGG AAAACCTGTT GACATGCCTG 
GCAGACCTTT TCCACAGCAT TGCCACGCAG AAGAAGAAGG TTGGCGTCAT CCCACCAAAG 
AAGTTCATTT CAAGGCTGAG GAAAGAGAAC GATCTCTTTG ATAACTACAT GCAGCAGGAT 
GCTCACGAGT TCTTAAATTA TTTGCTAAAC ACTATTGCGG ACATCCTGCA GGAAGAGAAG 
AAACAGGAAA AACAAAACGG GAAGTTAAAA AACGGCAACA TGAACGAACC AGCCGAGAAC 
AATAAGCCAG AACTCACCTG GGTCCACGAG ATTTTTCAGG GAACACTCAC TAACGAAACT 
CGATGCTTGA ACTGTGAAAC TGTGAGTAGC AAAGATGAAG ATTTTCTTGA CCTCTCTGTC 
GATGTGGAAC AGAACACCTC CATCACCCAC TGTCTGAGAG ACTTCAGCAA CACAGAAACG 
CTGTGTAGTG AACAAAAATA TTATTGTGAA ACATGTTGCA GCAAACAGGA GGCTCAGAAG 
AGGATGAGGG TAAAAAAGCT GCCCATGATT TTGGCCCTGC ACCTCAAGAG GTTCAAGTAC 
ATGGAACAGC TCCACAGGTA CACCAAGCTG TCTTACCGGG TGGTCTTCCC TCTGGAACTC 
CGGCTCTTCA ACACCTCCAG CGATGCTGTG AACCTGGACC GCATGTATGA CCTGGTTGCT 
GTGGTCGTTC ACTGTGGAAG TGGTCCTAAC CGTGGGCATT ACATCACCAT CGTAAAAAGT 
CATGGCTTCT GGCTGTTGTT TGATGACGAC ATTGTAGAGA AAATAGACGC TCAAGCCATT 
GAGGAATTCT ATGGTCTAAC GTCAGATATA TCAAAAAATT CAGAATCTGG ATATATTTTA 
TTCTATCAAT CAAGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808229.1
CDS	127..1227
Translation	MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

Target ORF information:

RefSeq Version	NM_177561.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 46 (Usp46), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177561.3

ORF Insert Sequence:

ATGACTGTCC GAAACATCGC CTCCATCTGT AATATGGGCA CCAATGCCTC TGCTCTGGAA 
AAAGACATTG GACCAGAGCA GTTTCCGATC AATGAACACT ACTTCGGATT GGTCAATTTT 
GGAAACACAT GCTACTGTAA CTCCGTGCTT CAAGCGCTGT ACTTCTGCCG GCCGTTCCGG 
GAGAATGTGT TGGCATACAA GGCCCAGCAA AAAAAGAAGG AAAACCTGTT GACATGCCTG 
GCAGACCTTT TCCACAGCAT TGCCACGCAG AAGAAGAAGG TTGGCGTCAT CCCACCAAAG 
AAGTTCATTT CAAGGCTGAG GAAAGAGAAC GATCTCTTTG ATAACTACAT GCAGCAGGAT 
GCTCACGAGT TCTTAAATTA TTTGCTAAAC ACTATTGCGG ACATCCTGCA GGAAGAGAAG 
AAACAGGAAA AACAAAACGG GAAGTTAAAA AACGGCAACA TGAACGAACC AGCCGAGAAC 
AATAAGCCAG AACTCACCTG GGTCCACGAG ATTTTTCAGG GAACACTCAC TAACGAAACT 
CGATGCTTGA ACTGTGAAAC TGTGAGTAGC AAAGATGAAG ATTTTCTTGA CCTCTCTGTC 
GATGTGGAAC AGAACACCTC CATCACCCAC TGTCTGAGAG ACTTCAGCAA CACAGAAACG 
CTGTGTAGTG AACAAAAATA TTATTGTGAA ACATGTTGCA GCAAACAGGA GGCTCAGAAG 
AGGATGAGGG TAAAAAAGCT GCCCATGATT TTGGCCCTGC ACCTCAAGAG GTTCAAGTAC 
ATGGAACAGC TCCACAGGTA CACCAAGCTG TCTTACCGGG TGGTCTTCCC TCTGGAACTC 
CGGCTCTTCA ACACCTCCAG CGATGCTGTG AACCTGGACC GCATGTATGA CCTGGTTGCT 
GTGGTCGTTC ACTGTGGAAG TGGTCCTAAC CGTGGGCATT ACATCACCAT CGTAAAAAGT 
CATGGCTTCT GGCTGTTGTT TGATGACGAC ATTGTAGAGA AAATAGACGC TCAAGCCATT 
GAGGAATTCT ATGGTCTAAC GTCAGATATA TCAAAAAATT CAGAATCTGG ATATATTTTA 
TTCTATCAAT CAAGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp46 ( XM_006504268.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504331.1 Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35307
Clone ID Related Accession (Same CDS sequence)	XM_006504268.3 , XM_006504268.4
Accession Version	XM_006504268.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 46 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504268.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTGTT TCCAGGGCAC CAATGCCTCT GCTCTGGAAA AAGACATTGG ACCAGAGCAG 
TTTCCGATCA ATGAACACTA CTTCGGATTG GTCAATTTTG GAAACACATG CTACTGTAAC 
TCCGTGCTTC AAGCGCTGTA CTTCTGCCGG CCGTTCCGGG AGAATGTGTT GGCATACAAG 
GCCCAGCAAA AAAAGAAGGA AAACCTGTTG ACATGCCTGG CAGACCTTTT CCACAGCATT 
GCCACGCAGA AGAAGAAGGT TGGCGTCATC CCACCAAAGA AGTTCATTTC AAGGCTGAGG 
AAAGAGAACG ATCTCTTTGA TAACTACATG CAGCAGGATG CTCACGAGTT CTTAAATTAT 
TTGCTAAACA CTATTGCGGA CATCCTGCAG GAAGAGAAGA AACAGGAAAA ACAAAACGGG 
AAGTTAAAAA ACGGCAACAT GAACGAACCA GCCGAGAACA ATAAGCCAGA ACTCACCTGG 
GTCCACGAGA TTTTTCAGGG AACACTCACT AACGAAACTC GATGCTTGAA CTGTGAAACT 
GTGAGTAGCA AAGATGAAGA TTTTCTTGAC CTCTCTGTCG ATGTGGAACA GAACACCTCC 
ATCACCCACT GTCTGAGAGA CTTCAGCAAC ACAGAAACGC TGTGTAGTGA ACAAAAATAT 
TATTGTGAAA CATGTTGCAG CAAACAGGAG GCTCAGAAGA GGATGAGGGT AAAAAAGCTG 
CCCATGATTT TGGCCCTGCA CCTCAAGAGG TTCAAGTACA TGGAACAGCT CCACAGGTAC 
ACCAAGCTGT CTTACCGGGT GGTCTTCCCT CTGGAACTCC GGCTCTTCAA CACCTCCAGC 
GATGCTGTGA ACCTGGACCG CATGTATGAC CTGGTTGCTG TGGTCGTTCA CTGTGGAAGT 
GGTCCTAACC GTGGGCATTA CATCACCATC GTAAAAAGTC ATGGCTTCTG GCTGTTGTTT 
GATGACGACA TTGTAGAGAA AATAGACGCT CAAGCCATTG AGGAATTCTA TGGTCTAACG 
TCAGATATAT CAAAAAATTC AGAATCTGGA TATATTTTAT TCTATCAATC AAGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504331.1
CDS	1577..2656
Translation	MNCFQGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

Target ORF information:

RefSeq Version	XM_006504268.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 46 (Usp46), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504268.4

ORF Insert Sequence:

ATGAACTGTT TCCAGGGCAC CAATGCCTCT GCTCTGGAAA AAGACATTGG ACCAGAGCAG 
TTTCCGATCA ATGAACACTA CTTCGGATTG GTCAATTTTG GAAACACATG CTACTGTAAC 
TCCGTGCTTC AAGCGCTGTA CTTCTGCCGG CCGTTCCGGG AGAATGTGTT GGCATACAAG 
GCCCAGCAAA AAAAGAAGGA AAACCTGTTG ACATGCCTGG CAGACCTTTT CCACAGCATT 
GCCACGCAGA AGAAGAAGGT TGGCGTCATC CCACCAAAGA AGTTCATTTC AAGGCTGAGG 
AAAGAGAACG ATCTCTTTGA TAACTACATG CAGCAGGATG CTCACGAGTT CTTAAATTAT 
TTGCTAAACA CTATTGCGGA CATCCTGCAG GAAGAGAAGA AACAGGAAAA ACAAAACGGG 
AAGTTAAAAA ACGGCAACAT GAACGAACCA GCCGAGAACA ATAAGCCAGA ACTCACCTGG 
GTCCACGAGA TTTTTCAGGG AACACTCACT AACGAAACTC GATGCTTGAA CTGTGAAACT 
GTGAGTAGCA AAGATGAAGA TTTTCTTGAC CTCTCTGTCG ATGTGGAACA GAACACCTCC 
ATCACCCACT GTCTGAGAGA CTTCAGCAAC ACAGAAACGC TGTGTAGTGA ACAAAAATAT 
TATTGTGAAA CATGTTGCAG CAAACAGGAG GCTCAGAAGA GGATGAGGGT AAAAAAGCTG 
CCCATGATTT TGGCCCTGCA CCTCAAGAGG TTCAAGTACA TGGAACAGCT CCACAGGTAC 
ACCAAGCTGT CTTACCGGGT GGTCTTCCCT CTGGAACTCC GGCTCTTCAA CACCTCCAGC 
GATGCTGTGA ACCTGGACCG CATGTATGAC CTGGTTGCTG TGGTCGTTCA CTGTGGAAGT 
GGTCCTAACC GTGGGCATTA CATCACCATC GTAAAAAGTC ATGGCTTCTG GCTGTTGTTT 
GATGACGACA TTGTAGAGAA AATAGACGCT CAAGCCATTG AGGAATTCTA TGGTCTAACG 
TCAGATATAT CAAAAAATTC AGAATCTGGA TATATTTTAT TCTATCAATC AAGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Proceedings of the National Academy of Sciences of the United States of America113(6)E705-14(2016 Feb)
Jahan AS,Lestra M,Swee LK,Fan Y,Lamers MM,Tafesse FG,Theile CS,Spooner E,Bruzzone R,Ploegh HL,Sanyal S

Impaired Maternal Behavior in Usp46 Mutant Mice: A Model for Trans-Generational Transmission of Maternal Care.
PloS one10(8)e0136016(2015)
Umemura S,Imai S,Mimura A,Fujiwara M,Ebihara S

Behavioral characteristics of ubiquitin-specific peptidase 46-deficient mice.
PloS one8(3)e58566(2013)
Imai S,Kano M,Nonoyama K,Ebihara S

Ubiquitin-specific peptidase 46 (Usp46) regulates mouse immobile behavior in the tail suspension test through the GABAergic system.
PloS one7(6)e39084(2012)
Imai S,Mamiya T,Tsukada A,Sakai Y,Mouri A,Nabeshima T,Ebihara S

Lysine 92 amino acid residue of USP46, a gene associated with 'behavioral despair' in mice, influences the deubiquitinating enzyme activity.
PloS one6(10)e26297(2011)
Zhang W,Tian QB,Li QK,Wang JM,Wang CN,Liu T,Liu DW,Wang MW