Spata4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spata4
Entrez Gene ID	69281
Full Name	spermatogenesis associated 4
Synonyms	1700001N01Rik,Srg2,TSARG2
General protein information	Preferred Names spermatogenesis associated 4 Names spermatogenesis-associated protein 4 spermatogenesis-related gene 2 protein testis and spermatogenesis cell-related protein 2 testis spermatocyte apoptosis-related gene 2 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_133711.4	NP_598472.2	spermatogenesis-associated protein 4
NM_133711.3	NP_598472.2	spermatogenesis-associated protein 4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPATA4	XP_005630109.1
Mus musculus (house mouse)	Spata4	NP_598472.2
Gallus gallus (chicken)	SPATA4	NP_001026309.1
Homo sapiens (human)	SPATA4	NP_653245.2
Pan troglodytes (chimpanzee)	SPATA4	NP_001008990.1
	SPATA4	NP_001030604.1
Danio rerio (zebrafish)	spata4	NP_001004013.1
Xenopus tropicalis (tropical clawed frog)	spata4	XP_004911229.1
Rattus norvegicus (Norway rat)	Spata4	NP_001002852.1
Bos taurus (cattle)	SPATA4	NP_001011677.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)

Spermatogenesis associated 4 promotes Sertoli cell proliferation modulated negatively by regulatory factor X1.
Jiang J, Zhang N, Shiba H, Li L, Wang Z
PloS one8(10)e75933(2013)

A yeast two hybrid screen identifies SPATA4 as a TRAPP interactor.
Duarte DT, Hul S, Sacher M
FEBS letters585(17)2676-81(2011 Sep)

Spata4 promotes osteoblast differentiation through Erk-activated Runx2 pathway.
Wang X, Harimoto K, Liu J, Guo J, Hinshaw S, Chang Z, Wang Z
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research26(8)1964-73(2011 Aug)

Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library.
Choi E, Lee J, Oh J, Park I, Han C, Yi C, Kim DH, Cho BN, Eddy EM, Cho C
BMC genomics8256(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RFX1 may have negative effect on cell proliferation of Sertoli cells via modulating Spata4 expression levels by binding the conserved 14-bp cis-elements of Spata4 promoter.
Title: Spermatogenesis associated 4 promotes Sertoli cell proliferation modulated negatively by regulatory factor X1.

Spata4 promotes osteoblast differentiation and mineralization through the Erk-activated Runx2 pathway.
Title: Spata4 promotes osteoblast differentiation through Erk-activated Runx2 pathway.

analysis of SPATA4 expression and variation in promotor sequences
Title: Molecular cloning and bioinformatic analysis of SPATA4 gene.

Srg2 gene was found up-regulated distinctly and playing an important role in cryptorchid testis.
Title: [Molecular cloning and expression in cryptorchid testis of SRG2 from a mouse testis spermatocyte apoptosis-related gene].

RT-PCR showed that SRG2 was expressed significantly in testis.
Title: [Molecular cloning of SRG2, a mouse testis spermatocyte apoptosis-related gene].

The following Spata4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spata4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10120	NM_133711.4 Latest version!	Mus musculus spermatogenesis associated 4 (Spata4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu10120	NM_133711.3	Mus musculus spermatogenesis associated 4 (Spata4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spata4 ( NM_133711.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598472.2 Mus musculus spermatogenesis associated 4 (Spata4), mRNA.

Price & Availability↑

CloneID	OMu10120
Clone ID Related Accession (Same CDS sequence)	NM_133711.4 , NM_133711.3
Accession Version	NM_133711.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Mus musculus(house mouse)
Product	spermatogenesis-associated protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102933.6. On Aug 22, 2018 this sequence version replaced NM_133711.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF395083.2, BC048650.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCG CCGGCCAGGC AGAGGAGTGT TTACCACTGC CGGCGGCAGA ATCATCCAAG 
ACGTCCTTAC CTACACCACC AGCAGTTCCC GCAGGAAAGA AGCCAAAGAA ATGTCTGGTG 
TATCCACATC CGCCTAGGAG CTCCCGCCTG AGTCGTTCTG TCCTGCGCTG GCTCCAGGGC 
CTAGATCTCA GCTTCTTCCC TAGGAATGTC ACCAGGGATT TTTCAAATGG CTACCTAGTT 
GCAGAGATAT TCTGTATTTA TTATCCCTGG GACCTTCGAT TATCATCCTT TGAAAATGGA 
ACCTCCTTAA AAGTCAAGTT GGATAACTGG GCACAGATAG AGAAGTTTCT AGCGAAAAAG 
AAGTTTAAAC TACCTAAAGA ACTAATCCAT GGAACAATTC ACTGTAAGGC TGGGGTACCT 
GAAATACTGA TACAGGAGAT TTACACTTTA CTAACACATC AAGAAATTAG AAGTATCCAG 
GATGATCTCG CTAATTTCAC AGACTATATT TACCAAATGC GTTTGCCCCT GGTTCCTAGG 
AATACAGTAT CAAAATCTAT TAAGAATAAC ATTAGGTTGT CGGAACTACT AAGCAATCCT 
AATGTACTCA GCAATGAACT CAAGATAGAA TTCCTCATCC TTTTGCAAAT GCTGCAAAGA 
AAATTAAGCA GAAAACTGAA TCCAGGGTGG TTTGATGTCA AGCCAACTGT GGGAGAAATC 
ACAATCGACC GTCTTCCTGC CCATAGCTAT AAGCGCAGAT ACAAGTCAAG AGGTTCAAAG 
GAGAAAGCTG CACAACCTTT ATCAAAATCA GACAATGATG GCAATGCACG GAAAGAAATC 
CATGTGAAGC AAAGTGGAAA TCCTTGTGAG AACACTGAAA ACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598472.2
CDS	21..908
Translation	MAAAGQAEECLPLPAAESSKTSLPTPPAVPAGKKPKKCLVYPHPPRSSRLSRSVLRWLQGLDLSFFPRNVTRDFSNGYLVAEIFCIYYPWDLRLSSFENGTSLKVKLDNWAQIEKFLAKKKFKLPKELIHGTIHCKAGVPEILIQEIYTLLTHQEIRSIQDDLANFTDYIYQMRLPLVPRNTVSKSIKNNIRLSELLSNPNVLSNELKIEFLILLQMLQRKLSRKLNPGWFDVKPTVGEITIDRLPAHSYKRRYKSRGSKEKAAQPLSKSDNDGNARKEIHVKQSGNPCENTENL

Target ORF information:

RefSeq Version	NM_133711.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spermatogenesis associated 4 (Spata4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133711.4

ORF Insert Sequence:

ATGGCTGCCG CCGGCCAGGC AGAGGAGTGT TTACCACTGC CGGCGGCAGA ATCATCCAAG 
ACGTCCTTAC CTACACCACC AGCAGTTCCC GCAGGAAAGA AGCCAAAGAA ATGTCTGGTG 
TATCCACATC CGCCTAGGAG CTCCCGCCTG AGTCGTTCTG TCCTGCGCTG GCTCCAGGGC 
CTAGATCTCA GCTTCTTCCC TAGGAATGTC ACCAGGGATT TTTCAAATGG CTACCTAGTT 
GCAGAGATAT TCTGTATTTA TTATCCCTGG GACCTTCGAT TATCATCCTT TGAAAATGGA 
ACCTCCTTAA AAGTCAAGTT GGATAACTGG GCACAGATAG AGAAGTTTCT AGCGAAAAAG 
AAGTTTAAAC TACCTAAAGA ACTAATCCAT GGAACAATTC ACTGTAAGGC TGGGGTACCT 
GAAATACTGA TACAGGAGAT TTACACTTTA CTAACACATC AAGAAATTAG AAGTATCCAG 
GATGATCTCG CTAATTTCAC AGACTATATT TACCAAATGC GTTTGCCCCT GGTTCCTAGG 
AATACAGTAT CAAAATCTAT TAAGAATAAC ATTAGGTTGT CGGAACTACT AAGCAATCCT 
AATGTACTCA GCAATGAACT CAAGATAGAA TTCCTCATCC TTTTGCAAAT GCTGCAAAGA 
AAATTAAGCA GAAAACTGAA TCCAGGGTGG TTTGATGTCA AGCCAACTGT GGGAGAAATC 
ACAATCGACC GTCTTCCTGC CCATAGCTAT AAGCGCAGAT ACAAGTCAAG AGGTTCAAAG 
GAGAAAGCTG CACAACCTTT ATCAAAATCA GACAATGATG GCAATGCACG GAAAGAAATC 
CATGTGAAGC AAAGTGGAAA TCCTTGTGAG AACACTGAAA ACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spata4 ( NM_133711.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598472.2 Mus musculus spermatogenesis associated 4 (Spata4), mRNA.

Price & Availability↑

CloneID	OMu10120
Clone ID Related Accession (Same CDS sequence)	NM_133711.4 , NM_133711.3
Accession Version	NM_133711.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Mus musculus(house mouse)
Product	spermatogenesis-associated protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF395083.2. On Apr 6, 2007 this sequence version replaced NM_133711.2. ##Evidence-Data-START## Transcript exon combination :: BC048650.1, AF395083.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCG CCGGCCAGGC AGAGGAGTGT TTACCACTGC CGGCGGCAGA ATCATCCAAG 
ACGTCCTTAC CTACACCACC AGCAGTTCCC GCAGGAAAGA AGCCAAAGAA ATGTCTGGTG 
TATCCACATC CGCCTAGGAG CTCCCGCCTG AGTCGTTCTG TCCTGCGCTG GCTCCAGGGC 
CTAGATCTCA GCTTCTTCCC TAGGAATGTC ACCAGGGATT TTTCAAATGG CTACCTAGTT 
GCAGAGATAT TCTGTATTTA TTATCCCTGG GACCTTCGAT TATCATCCTT TGAAAATGGA 
ACCTCCTTAA AAGTCAAGTT GGATAACTGG GCACAGATAG AGAAGTTTCT AGCGAAAAAG 
AAGTTTAAAC TACCTAAAGA ACTAATCCAT GGAACAATTC ACTGTAAGGC TGGGGTACCT 
GAAATACTGA TACAGGAGAT TTACACTTTA CTAACACATC AAGAAATTAG AAGTATCCAG 
GATGATCTCG CTAATTTCAC AGACTATATT TACCAAATGC GTTTGCCCCT GGTTCCTAGG 
AATACAGTAT CAAAATCTAT TAAGAATAAC ATTAGGTTGT CGGAACTACT AAGCAATCCT 
AATGTACTCA GCAATGAACT CAAGATAGAA TTCCTCATCC TTTTGCAAAT GCTGCAAAGA 
AAATTAAGCA GAAAACTGAA TCCAGGGTGG TTTGATGTCA AGCCAACTGT GGGAGAAATC 
ACAATCGACC GTCTTCCTGC CCATAGCTAT AAGCGCAGAT ACAAGTCAAG AGGTTCAAAG 
GAGAAAGCTG CACAACCTTT ATCAAAATCA GACAATGATG GCAATGCACG GAAAGAAATC 
CATGTGAAGC AAAGTGGAAA TCCTTGTGAG AACACTGAAA ACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598472.2
CDS	21..908
Translation	MAAAGQAEECLPLPAAESSKTSLPTPPAVPAGKKPKKCLVYPHPPRSSRLSRSVLRWLQGLDLSFFPRNVTRDFSNGYLVAEIFCIYYPWDLRLSSFENGTSLKVKLDNWAQIEKFLAKKKFKLPKELIHGTIHCKAGVPEILIQEIYTLLTHQEIRSIQDDLANFTDYIYQMRLPLVPRNTVSKSIKNNIRLSELLSNPNVLSNELKIEFLILLQMLQRKLSRKLNPGWFDVKPTVGEITIDRLPAHSYKRRYKSRGSKEKAAQPLSKSDNDGNARKEIHVKQSGNPCENTENL

Target ORF information:

RefSeq Version	NM_133711.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spermatogenesis associated 4 (Spata4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133711.3

ORF Insert Sequence:

ATGGCTGCCG CCGGCCAGGC AGAGGAGTGT TTACCACTGC CGGCGGCAGA ATCATCCAAG 
ACGTCCTTAC CTACACCACC AGCAGTTCCC GCAGGAAAGA AGCCAAAGAA ATGTCTGGTG 
TATCCACATC CGCCTAGGAG CTCCCGCCTG AGTCGTTCTG TCCTGCGCTG GCTCCAGGGC 
CTAGATCTCA GCTTCTTCCC TAGGAATGTC ACCAGGGATT TTTCAAATGG CTACCTAGTT 
GCAGAGATAT TCTGTATTTA TTATCCCTGG GACCTTCGAT TATCATCCTT TGAAAATGGA 
ACCTCCTTAA AAGTCAAGTT GGATAACTGG GCACAGATAG AGAAGTTTCT AGCGAAAAAG 
AAGTTTAAAC TACCTAAAGA ACTAATCCAT GGAACAATTC ACTGTAAGGC TGGGGTACCT 
GAAATACTGA TACAGGAGAT TTACACTTTA CTAACACATC AAGAAATTAG AAGTATCCAG 
GATGATCTCG CTAATTTCAC AGACTATATT TACCAAATGC GTTTGCCCCT GGTTCCTAGG 
AATACAGTAT CAAAATCTAT TAAGAATAAC ATTAGGTTGT CGGAACTACT AAGCAATCCT 
AATGTACTCA GCAATGAACT CAAGATAGAA TTCCTCATCC TTTTGCAAAT GCTGCAAAGA 
AAATTAAGCA GAAAACTGAA TCCAGGGTGG TTTGATGTCA AGCCAACTGT GGGAGAAATC 
ACAATCGACC GTCTTCCTGC CCATAGCTAT AAGCGCAGAT ACAAGTCAAG AGGTTCAAAG 
GAGAAAGCTG CACAACCTTT ATCAAAATCA GACAATGATG GCAATGCACG GAAAGAAATC 
CATGTGAAGC AAAGTGGAAA TCCTTGTGAG AACACTGAAA ACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)
Miyata H,Castaneda JM,Fujihara Y,Yu Z,Archambeault DR,Isotani A,Kiyozumi D,Kriseman ML,Mashiko D,Matsumura T,Matzuk RM,Mori M,Noda T,Oji A,Okabe M,Prunskaite-Hyyrylainen R,Ramirez-Solis R,Satouh Y,Zhang Q,Ikawa M,Matzuk MM

Spermatogenesis associated 4 promotes Sertoli cell proliferation modulated negatively by regulatory factor X1.
PloS one8(10)e75933(2013)
Jiang J,Zhang N,Shiba H,Li L,Wang Z

A yeast two hybrid screen identifies SPATA4 as a TRAPP interactor.
FEBS letters585(17)2676-81(2011 Sep)
Duarte DT,Hul S,Sacher M

Spata4 promotes osteoblast differentiation through Erk-activated Runx2 pathway.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research26(8)1964-73(2011 Aug)
Wang X,Harimoto K,Liu J,Guo J,Hinshaw S,Chang Z,Wang Z

Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library.
BMC genomics8256(2007 Jul)
Choi E,Lee J,Oh J,Park I,Han C,Yi C,Kim DH,Cho BN,Eddy EM,Cho C