Hspb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspb2
Entrez Gene ID	69253
Full Name	heat shock protein 2
Synonyms	27kDa,2810021G24Rik,HSP27,MKBP
General protein information	Preferred Names heat shock protein 2 Names heat shock protein beta-2 heat shock 27kD protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A5.3\|9 27.75 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164708.1	NP_001158180.1	heat shock protein beta-2 isoform 2
NM_024441.3	NP_077761.3	heat shock protein beta-2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hspb2	NP_569115.1
Homo sapiens (human)	HSPB2	NP_001532.1
Canis lupus familiaris (dog)	HSPB2	XP_854111.1
Mus musculus (house mouse)	Hspb2	NP_077761.3
Danio rerio (zebrafish)	hspb2	NP_001017744.1
Xenopus tropicalis (tropical clawed frog)	hspb2	XP_002932983.1
	HSPB2	XP_002799806.1
Bos taurus (cattle)	HSPB2	NP_001091850.1
Gallus gallus (chicken)	HSPB2	NP_001001527.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial deficits and abnormal mitochondrial retrograde axonal transport play a role in the pathogenesis of mutant Hsp27-induced Charcot Marie Tooth Disease.
Kalmar B, Innes A, Wanisch K, Kolaszynska AK, Pandraud A, Kelly G, Abramov AY, Reilly MM, Schiavo G, Greensmith L
Human molecular genetics26(17)3313-3326(2017 Sep)

HSP27 regulates TGF-? mediated lung fibroblast differentiation through the Smad3 and ERK pathways.
Wang G, Jiao H, Zheng JN, Sun X
International journal of molecular medicine39(1)183-190(2017 Jan)

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found significantly slower retrograde transport of mitochondria in Ser135Phe, Pro39Leu and Arg140Gly mutant Hsp27 expressing motor neurons than in wild type Hsp27 neurons, although anterograde movement velocities remained normal.
Title: Mitochondrial deficits and abnormal mitochondrial retrograde axonal transport play a role in the pathogenesis of mutant Hsp27-induced Charcot Marie Tooth Disease.

HSP27 expression is upregulated in lung fibroblasts during pulmonary fibrosis, and subsequently, HSP27 modulates lung fibroblast differentiation through the Smad3 and ERK pathways.
Title: HSP27 regulates TGF-? mediated lung fibroblast differentiation through the Smad3 and ERK pathways.

phosphorylated Hsp27 stimulates autophagy and lipid droplet clearance and interacts with STAT3.
Title: Phosphorylated heat shock protein 27 promotes lipid clearance in hepatic cells through interacting with STAT3 and activating autophagy.

Heat shock proteins 27 and 70 were involved in the protective effect.
Title: Heat shock proteins 27 and 70 contribute to the protection of Schisandrin B against d-galactosamine-induced liver injury in mice.

HspB2 has roles in maintaining ATP levels and as well as chaperone-dependent functions for metabolic homeostasis
Title: Characterization of the Cardiac Overexpression of HSPB2 Reveals Mitochondrial and Myogenic Roles Supported by a Cardiac HspB2 Interactome.

The following Hspb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22588	NM_001164708.1 Latest version!	Mus musculus heat shock protein 2 (Hspb2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu22598	NM_024441.3 Latest version!	Mus musculus heat shock protein 2 (Hspb2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspb2 ( NM_001164708.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001158180.1 Mus musculus heat shock protein 2 (Hspb2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22588
Clone ID Related Accession (Same CDS sequence)	NM_001164708.1
Accession Version	NM_001164708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	heat shock protein beta-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012780.1 and BU704695.1. Transcript Variant: This variant (2) uses a different splice site, compared to variant 1. The resulting protein (isoform 2) is missing an internal segment when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK012780.1, BY711895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGCC GCACAGTGCC ACACGCCCAC CCAGCCACTG CCGAGTACGA ATTTGCCAAC 
CCCAGCCGTC TGGGCGAGCA GCGCTTCGGA GAAGATGTGG ACCCCTGGCG GGTTCGAGCT 
GCTCTATCCC ATGATGGCAT CCTTAACTTG GAGGCGCCGC GGGGTGGCCG GCATTTGGAC 
ACGGAAGTCA ATGAAGTCTA CATCTCCCTG CTTCCTGCTC CTCCTGACCC CGAGGAAGAG 
GAAGAGATAG CCAGAGTTGA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158180.1
CDS	85..351
Translation	MSGRTVPHAHPATAEYEFANPSRLGEQRFGEDVDPWRVRAALSHDGILNLEAPRGGRHLDTEVNEVYISLLPAPPDPEEEEEIARVEP

Target ORF information:

RefSeq Version	NM_001164708.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 2 (Hspb2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164708.1

ORF Insert Sequence:

ATGTCGGGCC GCACAGTGCC ACACGCCCAC CCAGCCACTG CCGAGTACGA ATTTGCCAAC 
CCCAGCCGTC TGGGCGAGCA GCGCTTCGGA GAAGATGTGG ACCCCTGGCG GGTTCGAGCT 
GCTCTATCCC ATGATGGCAT CCTTAACTTG GAGGCGCCGC GGGGTGGCCG GCATTTGGAC 
ACGGAAGTCA ATGAAGTCTA CATCTCCCTG CTTCCTGCTC CTCCTGACCC CGAGGAAGAG 
GAAGAGATAG CCAGAGTTGA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspb2 ( NM_024441.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077761.3 Mus musculus heat shock protein 2 (Hspb2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22598
Clone ID Related Accession (Same CDS sequence)	NM_024441.3
Accession Version	NM_024441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	heat shock protein beta-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012780.1, BC064051.1 and BU704695.1. On Sep 11, 2009 this sequence version replaced NM_024441.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064051.1, CB587574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGCC GCACAGTGCC ACACGCCCAC CCAGCCACTG CCGAGTACGA ATTTGCCAAC 
CCCAGCCGTC TGGGCGAGCA GCGCTTCGGA GAAGGCCTCC TGCCAGAAGA GATCCTGACC 
CCCACCCTCT ATCACGGCTA CTATGTTCGG CCTCGGGCCG CCAGAGCTGG CGAGGGCGCC 
AGGGCAGGGG CCTCAGAGCT CAGGCTCAGT GAAGGCAAGT TCCAGGCGTT TCTGGATGTG 
AGCCACTTTA CCCCAGATGA GGTGACGGTG AGGACTGTGG ATAACCTGCT GGAGGTGTCT 
GCCCGACACC CCCAGCGTCT GGATCGCCAC GGCTTCGTGT CCCGAGAGTT CTGTCGCACC 
TATGTCCTGC CTGCAGATGT GGACCCCTGG CGGGTTCGAG CTGCTCTATC CCATGATGGC 
ATCCTTAACT TGGAGGCGCC GCGGGGTGGC CGGCATTTGG ACACGGAAGT CAATGAAGTC 
TACATCTCCC TGCTTCCTGC TCCTCCTGAC CCCGAGGAAG AGGAAGAGAT AGCCAGAGTT 
GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077761.3
CDS	85..633
Translation	MSGRTVPHAHPATAEYEFANPSRLGEQRFGEGLLPEEILTPTLYHGYYVRPRAARAGEGARAGASELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYVLPADVDPWRVRAALSHDGILNLEAPRGGRHLDTEVNEVYISLLPAPPDPEEEEEIARVEP

Target ORF information:

RefSeq Version	NM_024441.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 2 (Hspb2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024441.3

ORF Insert Sequence:

ATGTCGGGCC GCACAGTGCC ACACGCCCAC CCAGCCACTG CCGAGTACGA ATTTGCCAAC 
CCCAGCCGTC TGGGCGAGCA GCGCTTCGGA GAAGGCCTCC TGCCAGAAGA GATCCTGACC 
CCCACCCTCT ATCACGGCTA CTATGTTCGG CCTCGGGCCG CCAGAGCTGG CGAGGGCGCC 
AGGGCAGGGG CCTCAGAGCT CAGGCTCAGT GAAGGCAAGT TCCAGGCGTT TCTGGATGTG 
AGCCACTTTA CCCCAGATGA GGTGACGGTG AGGACTGTGG ATAACCTGCT GGAGGTGTCT 
GCCCGACACC CCCAGCGTCT GGATCGCCAC GGCTTCGTGT CCCGAGAGTT CTGTCGCACC 
TATGTCCTGC CTGCAGATGT GGACCCCTGG CGGGTTCGAG CTGCTCTATC CCATGATGGC 
ATCCTTAACT TGGAGGCGCC GCGGGGTGGC CGGCATTTGG ACACGGAAGT CAATGAAGTC 
TACATCTCCC TGCTTCCTGC TCCTCCTGAC CCCGAGGAAG AGGAAGAGAT AGCCAGAGTT 
GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial deficits and abnormal mitochondrial retrograde axonal transport play a role in the pathogenesis of mutant Hsp27-induced Charcot Marie Tooth Disease.
Human molecular genetics26(17)3313-3326(2017 Sep)
Kalmar B,Innes A,Wanisch K,Kolaszynska AK,Pandraud A,Kelly G,Abramov AY,Reilly MM,Schiavo G,Greensmith L

HSP27 regulates TGF-? mediated lung fibroblast differentiation through the Smad3 and ERK pathways.
International journal of molecular medicine39(1)183-190(2017 Jan)
Wang G,Jiao H,Zheng JN,Sun X

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH