G6pc3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	G6pc3
Entrez Gene ID	68401
Full Name	glucose 6 phosphatase, catalytic, 3
Synonyms	0710001K01Rik,AU019276,AU045429,AV128920,AW545836,UGRP
General protein information	Preferred Names glucose 6 phosphatase, catalytic, 3 Names glucose-6-phosphatase 3 G-6-Pase 3 G6Pase 3 glucose-6-phosphatase catalytic subunit-related ubiquitous glucose-6-phosphatase catalytic subunit-related protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_175935.3	NP_787949.2	glucose-6-phosphatase 3

Pathways from BioSystems

Homologies

	G6PC3	XP_001114234.1
Canis lupus familiaris (dog)	G6PC3	XP_548065.2
Gallus gallus (chicken)	G6PC3	XP_003643859.1
Pan troglodytes (chimpanzee)	G6PC3	XP_511541.3
Xenopus tropicalis (tropical clawed frog)	g6pc3.1	XP_004918642.1
Homo sapiens (human)	G6PC3	NP_612396.1
Bos taurus (cattle)	G6PC3	NP_899208.2
Mus musculus (house mouse)	G6pc3	NP_787949.2
Rattus norvegicus (Norway rat)	G6pc3	NP_788266.2
Danio rerio (zebrafish)	g6pc3	NP_001073535.1

Gene Ontology
Bibliography

Related articles in PubMed

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

14-3-3? coordinates adipogenesis of visceral fat.
Lim GE, Albrecht T, Piske M, Sarai K, Lee JT, Ramshaw HS, Sinha S, Guthridge MA, Acker-Palmer A, Lopez AF, Clee SM, Nislow C, Johnson JD
Nature communications67671(2015 Jul)

Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-? (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation.
Gautam S, Kirschnek S, Gentle IE, Kopiniok C, Henneke P, H?cker H, Malleret L, Belaaouaj A, H?cker G
Cell death and differentiation20(8)1068-79(2013 Aug)

Glucose-6-phosphatase-?, implicated in a congenital neutropenia syndrome, is essential for macrophage energy homeostasis and functionality.
Jun HS, Cheung YY, Lee YM, Mansfield BC, Chou JY
Blood119(17)4047-55(2012 Apr)

G-CSF improves murine G6PC3-deficient neutrophil function by modulating apoptosis and energy homeostasis.
Jun HS, Lee YM, Song KD, Mansfield BC, Chou JY
Blood117(14)3881-92(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-beta (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation.
Title: Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-? (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation.

G-CSF improves G6pc3(-/-) neutrophil survival by modulating apoptotic mediators and rectifies function by enhancing energy homeostasis.
Title: G-CSF improves murine G6PC3-deficient neutrophil function by modulating apoptosis and energy homeostasis.

in nonapoptotic neutrophils, G6PC3 is essential for normal energy homeostasis. A G6PC3 deficiency prevents recycling of ER glucose to the cytoplasm, leading to neutrophil dysfunction
Title: Lack of glucose recycling between endoplasmic reticulum and cytoplasm underlies cellular dysfunction in glucose-6-phosphatase-beta-deficient neutrophils in a congenital neutropenia syndrome.

The absence of G6Pase-beta led to neutropenia
Title: Impaired neutrophil activity and increased susceptibility to bacterial infection in mice lacking glucose-6-phosphatase-beta.

UGRP/G6Pase is the major glucose-6-phosphatase of physiological importance for glucose homeostasis in vivo.
Title: Deletion of the gene encoding the ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein (UGRP)/glucose-6-phosphatase catalytic subunit-beta results in lowered plasma cholesterol and elevated glucagon.

The following G6pc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G6pc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06210	NM_175935.3 Latest version!	Mus musculus glucose 6 phosphatase, catalytic, 3 (G6pc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

G6pc3 ( NM_175935.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_787949.2 Mus musculus glucose 6 phosphatase, catalytic, 3 (G6pc3), mRNA.

Price & Availability↑

CloneID	OMu06210
Clone ID Related Accession (Same CDS sequence)	NM_175935.3
Accession Version	NM_175935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	glucose-6-phosphatase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC069959.1. On May 16, 2004 this sequence version replaced NM_175935.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069959.1, AK165395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCCA CGCTGAGCGC GGGCATCATA ATGGCTGAAG CACTACAGAA CCGGCTTCCC 
GGGCTAGAGA ATATGTGGCT CTGGGTCACC TTTCTGGGCG ATCCTAAGAA TCTTTTTCAG 
TTCTGCTTCC CCGCGGCCTA CTACGCCTCC CGCCGCCTGG GCATCTCCGT GCTCTGGATC 
ACCTTCATTG CTGAGTGGCT CAACCTTGTC TTCAAGTGGT TTCTGTTTGG AGACAGGCCC 
TTTTGGTGGG TGCATGAATC CGGGTACTCC ACCCAGACTC CAATCCAGAT TCACCAGTTC 
CCTTCTTCTT GTGAGACTGG TCCAGGCAGC CCCTCCGGCC ACTGCATGAT CACAGGCGCA 
GCTCTTTGGC CTGTAATGAC GGCCATTTCT TCTCAGGTGG CTTCTCGGTC CCGCAGCCCC 
TGGGTGAGGG TGATTCCTGG CCTGGCTTAT TGTACCTTCC TATTGGCAGT CGGCCTATCT 
CGGGTCTTCC TCTTAGCACA TTTCCCTCAC CAAGTGTTGG GCGGCCTTAT TGTTGGTGCT 
GCCCTTGGCT GGCTAATGAG CCCCCGGGTA CCCATGGAGC GGGAGCTTAG CTTTTATGGG 
TTGACTGCTC TGGCCCTCAT GCTGGGTGCC AGCCTCATGT ATTGGACTCT CTTTACACTG 
GGCCTAGATC TTTCCTGGTC CATCAACCTG GCTTCCAAGT GGTGTGAACG GCCCGAGTGG 
GTGCACATGG ACAGTCGGCC TTTTGCCTCA CTGAGCCGTG ACTCAGGATC TGCCCTGGGT 
CTGGGCATTG CCCTCCACAC TCCCTGCTAT GCCCAGATAC GGCGGGCGCA CCTAGGGAAT 
GGCCAGAAAA TAGCTTGCTT TGTGCTGGCC ATGGGGCTGC TGGTCTTCCT GGAATGGCTG 
GGCTACCCAC CTCAGATCAG CCTCTTCTAC ATCTTCAACT TCCTCAAGTA TACCCTCTGG 
CCGTGCCTGG TCTTGGCCCT TGTGCCCTGG GTGGTGCACA CATTGAGTGA CCAGGAAGCA 
CCGCCCATTC GCTCCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787949.2
CDS	228..1268
Translation	MESTLSAGIIMAEALQNRLPGLENMWLWVTFLGDPKNLFQFCFPAAYYASRRLGISVLWITFIAEWLNLVFKWFLFGDRPFWWVHESGYSTQTPIQIHQFPSSCETGPGSPSGHCMITGAALWPVMTAISSQVASRSRSPWVRVIPGLAYCTFLLAVGLSRVFLLAHFPHQVLGGLIVGAALGWLMSPRVPMERELSFYGLTALALMLGASLMYWTLFTLGLDLSWSINLASKWCERPEWVHMDSRPFASLSRDSGSALGLGIALHTPCYAQIRRAHLGNGQKIACFVLAMGLLVFLEWLGYPPQISLFYIFNFLKYTLWPCLVLALVPWVVHTLSDQEAPPIRSS

Target ORF information:

RefSeq Version	NM_175935.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glucose 6 phosphatase, catalytic, 3 (G6pc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175935.3

ORF Insert Sequence:

ATGGAGTCCA CGCTGAGCGC GGGCATCATA ATGGCTGAAG CACTACAGAA CCGGCTTCCC 
GGGCTAGAGA ATATGTGGCT CTGGGTCACC TTTCTGGGCG ATCCTAAGAA TCTTTTTCAG 
TTCTGCTTCC CCGCGGCCTA CTACGCCTCC CGCCGCCTGG GCATCTCCGT GCTCTGGATC 
ACCTTCATTG CTGAGTGGCT CAACCTTGTC TTCAAGTGGT TTCTGTTTGG AGACAGGCCC 
TTTTGGTGGG TGCATGAATC CGGGTACTCC ACCCAGACTC CAATCCAGAT TCACCAGTTC 
CCTTCTTCTT GTGAGACTGG TCCAGGCAGC CCCTCCGGCC ACTGCATGAT CACAGGCGCA 
GCTCTTTGGC CTGTAATGAC GGCCATTTCT TCTCAGGTGG CTTCTCGGTC CCGCAGCCCC 
TGGGTGAGGG TGATTCCTGG CCTGGCTTAT TGTACCTTCC TATTGGCAGT CGGCCTATCT 
CGGGTCTTCC TCTTAGCACA TTTCCCTCAC CAAGTGTTGG GCGGCCTTAT TGTTGGTGCT 
GCCCTTGGCT GGCTAATGAG CCCCCGGGTA CCCATGGAGC GGGAGCTTAG CTTTTATGGG 
TTGACTGCTC TGGCCCTCAT GCTGGGTGCC AGCCTCATGT ATTGGACTCT CTTTACACTG 
GGCCTAGATC TTTCCTGGTC CATCAACCTG GCTTCCAAGT GGTGTGAACG GCCCGAGTGG 
GTGCACATGG ACAGTCGGCC TTTTGCCTCA CTGAGCCGTG ACTCAGGATC TGCCCTGGGT 
CTGGGCATTG CCCTCCACAC TCCCTGCTAT GCCCAGATAC GGCGGGCGCA CCTAGGGAAT 
GGCCAGAAAA TAGCTTGCTT TGTGCTGGCC ATGGGGCTGC TGGTCTTCCT GGAATGGCTG 
GGCTACCCAC CTCAGATCAG CCTCTTCTAC ATCTTCAACT TCCTCAAGTA TACCCTCTGG 
CCGTGCCTGG TCTTGGCCCT TGTGCCCTGG GTGGTGCACA CATTGAGTGA CCAGGAAGCA 
CCGCCCATTC GCTCCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

14-3-3? coordinates adipogenesis of visceral fat.
Nature communications67671(2015 Jul)
Lim GE,Albrecht T,Piske M,Sarai K,Lee JT,Ramshaw HS,Sinha S,Guthridge MA,Acker-Palmer A,Lopez AF,Clee SM,Nislow C,Johnson JD

Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-? (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation.
Cell death and differentiation20(8)1068-79(2013 Aug)
Gautam S,Kirschnek S,Gentle IE,Kopiniok C,Henneke P,H?cker H,Malleret L,Belaaouaj A,H?cker G

Glucose-6-phosphatase-?, implicated in a congenital neutropenia syndrome, is essential for macrophage energy homeostasis and functionality.
Blood119(17)4047-55(2012 Apr)
Jun HS,Cheung YY,Lee YM,Mansfield BC,Chou JY

G-CSF improves murine G6PC3-deficient neutrophil function by modulating apoptosis and energy homeostasis.
Blood117(14)3881-92(2011 Apr)
Jun HS,Lee YM,Song KD,Mansfield BC,Chou JY