Cdt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdt1
Entrez Gene ID	67177
Full Name	chromatin licensing and DNA replication factor 1
Synonyms	2610318F11Rik,AW545653,C76791,Ris2
General protein information	Preferred Names chromatin licensing and DNA replication factor 1 Names DNA replication factor Cdt1 DUP cdt1 double parked homolog retroviral insertion site 2 protein retroviral integration site 1 retroviral integration site 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026014.3	NP_080290.3	DNA replication factor Cdt1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CDT1	NP_112190.2
Pan troglodytes (chimpanzee)	CDT1	XP_003315291.1
Rattus norvegicus (Norway rat)	Cdt1	NP_001099662.1
Danio rerio (zebrafish)	cdt1	XP_695164.3
Canis lupus familiaris (dog)	CDT1	XP_536753.3
Bos taurus (cattle)	CDT1	XP_005218826.1
Mus musculus (house mouse)	Cdt1	NP_080290.3
Gallus gallus (chicken)	CDT1	XP_423919.4
Xenopus tropicalis (tropical clawed frog)	cdt1	XP_002933700.1

Gene Ontology
Bibliography

Related articles in PubMed

Geminin is an indispensable inhibitor of Cdt1 in mouse embryonic stem cells.
Hosogane M, Bosu L, Fukumoto E, Yamada H, Sato S, Nakayama K
Genes to cells : devoted to molecular & cellular mechanisms22(4)360-375(2017 Apr)

Characterization of conserved arginine residues on Cdt1 that affect licensing activity and interaction with Geminin or Mcm complex.
You Z, Ode KL, Shindo M, Takisawa H, Masai H
Cell cycle (Georgetown, Tex.)15(9)1213-26(2016 May)

The apical complex protein Pals1 is required to maintain cerebellar progenitor cells in a proliferative state.
Park JY, Hughes LJ, Moon UY, Park R, Kim SB, Tran K, Lee JS, Cho SH, Kim S
Development (Cambridge, England)143(1)133-46(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that ablation of Geminin induces massive rereplication as a result of unrestrained Cdt1 activity in embryonic stem cells, whereas it has no such effect in embryonic fibroblasts in which alternative regulation of Cdt1 activity is intact.
Title: Geminin is an indispensable inhibitor of Cdt1 in mouse embryonic stem cells.

conserved arginine residues play critical roles in interaction with Geminin and Mcm that are crucial for proper conformation of the complexes and its licensing activity.
Title: Characterization of conserved arginine residues on Cdt1 that affect licensing activity and interaction with Geminin or Mcm complex.

This study reveals a conserved new regulatory Cdt1 domain crucial for proper DNA licensing activity.
Title: A spontaneous Cdt1 mutation in 129 mouse strains reveals a regulatory domain restraining replication licensing.

Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins.
Title: Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins.

Determined the structures of mCdt1CS (mCdt1C_small; residues 452 to 557) and mCdt1CL (mCdt1C_large; residues 420 to 557) using X-ray crystallography and NMR. This study reveals that Cdt1 is formed with a tandem repeat of the winged helix domain.
Title: Structure of the Cdt1 C-terminal domain: conservation of the winged helix fold in replication licensing factors.

The following Cdt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01414	NM_026014.3 Latest version!	Mus musculus chromatin licensing and DNA replication factor 1 (Cdt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$328.30 $469.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdt1 ( NM_026014.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080290.3 Mus musculus chromatin licensing and DNA replication factor 1 (Cdt1), mRNA.

Price & Availability↑

CloneID	OMu01414
Clone ID Related Accession (Same CDS sequence)	NM_026014.3
Accession Version	NM_026014.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	DNA replication factor Cdt1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC048076.1. On Jun 19, 2003 this sequence version replaced NM_026014.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048076.1, AK012041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAAA GTCGTGTTAC CGATTTCTAT GCGTGCCGTC GCCCTGGCCT TACGACTCCG 
CGGGCCAAGT CGATCTGTCT CACCCCGAGC CCTGGTGGCC TCGTGGCTCC TGCGTTCACC 
CGGAGCAGCA GCCGCAAGCG CGCCCGGCCC CCAGCCGAAC CCGGGAGTGA CCAGCCCGCG 
CCGCTCGCGC GCCGGAGGTT ACGGCTGCCT GGATTGGACT CCTGCCCCAG TTCTCTGCCT 
GAGCCCAGTT CCCCAGCTGA GCCCAGCCCT CCAGCTGACC CTAGCCCTCC AGCTGACCCT 
GGCTCCCCCG TTTGCCCATC CCCCGTCAAG CGAACAAAGA GTACAACTGT TTATGTTGGT 
CAACAGCCGG GCAAGATCCC CTCAGAGGAC TCCGTCTCTG AGCTCCAGTC CTGCCTGAGG 
CGGGCACGGA AGTTGGGAGC CCAGGCACGG GCCCTGAGAG CCCGAGTCCA AGAGAATGCT 
GTGGAGCCTA GTACCCCAGA TGCCAAGGTG CCCACAGAGC AACCATGTGT CGAGAAAGCT 
CCTGCCTACC AGCGCTTCCA TGCTCTGGCT CAGCCTGGTC TCCCAGGCCT TGTCCTACCC 
TACAAGTATC AGGTGCTAGT TGAGATGTTC CGCAGCATGG ACACCATTGT GAGCATGCTC 
CACAATCGCT CTGAGACTGT GACCTTTGCC AAAGTCAAGC AAGGTGTTCA GGAGATGATG 
CGCAAGCGCT TTGAAGAGCG CAATGTGGGC CAGATCAAAA CCGTGTATCC CACGTCGTAT 
CGCTTCCGCC AGGAGTGCAA TGTCCCCACC TTCAAGGACA GCATCAAGAG ATCTGATTAC 
CAGCTCACCA TCGAGCCCTT GCTGGGCCAG GAGGCTGGCG GTGCCACCCA GCTCACAGCC 
ACGTGCCTCC TGCAGCGCCG GCAAGTCTTC CGGCAGAACC TGGTGGAACG TGTCAAGGAA 
CAGCACAAGG TCTTCCTGGC TTCACTGAAC CCCCCCATGG CGGTGCCGGA CGACCAGCTG 
ACCCGCTGGC ATCCGCGCTT CAATGTGGAC GAGGTGCCTG ACATTGAGCC AGCTGAACTG 
CCCCAGCCTC CTGTCACAGA GAAGCTCACC ACTGCCCAGG AAGTGCTGGC CCGTGCCCGG 
AGCTTGATGA CACCCAAGAT GGAGAAGGCC CTGAGCAACC TGGCCCTGCG CTCGGCTGAG 
CCCGGTAGCC CTGGGACCTC TACTCCACCA CTCCCGGCCA CTCCGCCAGC CACCCCACCT 
GCTGCCTCTC CGAGCGCCCT GAAGGGTGTG TCCCAAGCAC TGCTAGAGCG GATAAGGGCC 
AAGGAGGTCC AGAAGCAGCT GGCAAGGATG ACACGGTGCC CCGAGCAGGA GCTTCGCCTG 
CAGCGGTTAG AGCGTCTGCC AGAGCTGGCC CGCGTGCTGC GCAATGTCTT CGTGTCTGAG 
CGGAAGCCGG CACTCACTAT GGAGGTGGTC TGTGCAAGGA TGGTGGACAG TTGCCAAACT 
GCTCTGAGTC CAGGGGAGAT GGAGAAACAT CTGGTGCTCC TGGCAGAGTT GCTGCCGGAC 
TGGCTCAGCC TGCATCGCAT CCGCACGGAT ACCTACGTCA AGCTGGACAA GGCTGTTGAC 
CTGGCTGGCC TCACTGCGAG GCTGGCCCAC CACGTCCACG CCGAGGGGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080290.3
CDS	42..1715
Translation	MAQSRVTDFYACRRPGLTTPRAKSICLTPSPGGLVAPAFTRSSSRKRARPPAEPGSDQPAPLARRRLRLPGLDSCPSSLPEPSSPAEPSPPADPSPPADPGSPVCPSPVKRTKSTTVYVGQQPGKIPSEDSVSELQSCLRRARKLGAQARALRARVQENAVEPSTPDAKVPTEQPCVEKAPAYQRFHALAQPGLPGLVLPYKYQVLVEMFRSMDTIVSMLHNRSETVTFAKVKQGVQEMMRKRFEERNVGQIKTVYPTSYRFRQECNVPTFKDSIKRSDYQLTIEPLLGQEAGGATQLTATCLLQRRQVFRQNLVERVKEQHKVFLASLNPPMAVPDDQLTRWHPRFNVDEVPDIEPAELPQPPVTEKLTTAQEVLARARSLMTPKMEKALSNLALRSAEPGSPGTSTPPLPATPPATPPAASPSALKGVSQALLERIRAKEVQKQLARMTRCPEQELRLQRLERLPELARVLRNVFVSERKPALTMEVVCARMVDSCQTALSPGEMEKHLVLLAELLPDWLSLHRIRTDTYVKLDKAVDLAGLTARLAHHVHAEGL

Target ORF information:

RefSeq Version	NM_026014.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromatin licensing and DNA replication factor 1 (Cdt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026014.3

ORF Insert Sequence:

ATGGCGCAAA GTCGTGTTAC CGATTTCTAT GCGTGCCGTC GCCCTGGCCT TACGACTCCG 
CGGGCCAAGT CGATCTGTCT CACCCCGAGC CCTGGTGGCC TCGTGGCTCC TGCGTTCACC 
CGGAGCAGCA GCCGCAAGCG CGCCCGGCCC CCAGCCGAAC CCGGGAGTGA CCAGCCCGCG 
CCGCTCGCGC GCCGGAGGTT ACGGCTGCCT GGATTGGACT CCTGCCCCAG TTCTCTGCCT 
GAGCCCAGTT CCCCAGCTGA GCCCAGCCCT CCAGCTGACC CTAGCCCTCC AGCTGACCCT 
GGCTCCCCCG TTTGCCCATC CCCCGTCAAG CGAACAAAGA GTACAACTGT TTATGTTGGT 
CAACAGCCGG GCAAGATCCC CTCAGAGGAC TCCGTCTCTG AGCTCCAGTC CTGCCTGAGG 
CGGGCACGGA AGTTGGGAGC CCAGGCACGG GCCCTGAGAG CCCGAGTCCA AGAGAATGCT 
GTGGAGCCTA GTACCCCAGA TGCCAAGGTG CCCACAGAGC AACCATGTGT CGAGAAAGCT 
CCTGCCTACC AGCGCTTCCA TGCTCTGGCT CAGCCTGGTC TCCCAGGCCT TGTCCTACCC 
TACAAGTATC AGGTGCTAGT TGAGATGTTC CGCAGCATGG ACACCATTGT GAGCATGCTC 
CACAATCGCT CTGAGACTGT GACCTTTGCC AAAGTCAAGC AAGGTGTTCA GGAGATGATG 
CGCAAGCGCT TTGAAGAGCG CAATGTGGGC CAGATCAAAA CCGTGTATCC CACGTCGTAT 
CGCTTCCGCC AGGAGTGCAA TGTCCCCACC TTCAAGGACA GCATCAAGAG ATCTGATTAC 
CAGCTCACCA TCGAGCCCTT GCTGGGCCAG GAGGCTGGCG GTGCCACCCA GCTCACAGCC 
ACGTGCCTCC TGCAGCGCCG GCAAGTCTTC CGGCAGAACC TGGTGGAACG TGTCAAGGAA 
CAGCACAAGG TCTTCCTGGC TTCACTGAAC CCCCCCATGG CGGTGCCGGA CGACCAGCTG 
ACCCGCTGGC ATCCGCGCTT CAATGTGGAC GAGGTGCCTG ACATTGAGCC AGCTGAACTG 
CCCCAGCCTC CTGTCACAGA GAAGCTCACC ACTGCCCAGG AAGTGCTGGC CCGTGCCCGG 
AGCTTGATGA CACCCAAGAT GGAGAAGGCC CTGAGCAACC TGGCCCTGCG CTCGGCTGAG 
CCCGGTAGCC CTGGGACCTC TACTCCACCA CTCCCGGCCA CTCCGCCAGC CACCCCACCT 
GCTGCCTCTC CGAGCGCCCT GAAGGGTGTG TCCCAAGCAC TGCTAGAGCG GATAAGGGCC 
AAGGAGGTCC AGAAGCAGCT GGCAAGGATG ACACGGTGCC CCGAGCAGGA GCTTCGCCTG 
CAGCGGTTAG AGCGTCTGCC AGAGCTGGCC CGCGTGCTGC GCAATGTCTT CGTGTCTGAG 
CGGAAGCCGG CACTCACTAT GGAGGTGGTC TGTGCAAGGA TGGTGGACAG TTGCCAAACT 
GCTCTGAGTC CAGGGGAGAT GGAGAAACAT CTGGTGCTCC TGGCAGAGTT GCTGCCGGAC 
TGGCTCAGCC TGCATCGCAT CCGCACGGAT ACCTACGTCA AGCTGGACAA GGCTGTTGAC 
CTGGCTGGCC TCACTGCGAG GCTGGCCCAC CACGTCCACG CCGAGGGGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Geminin is an indispensable inhibitor of Cdt1 in mouse embryonic stem cells.
Genes to cells : devoted to molecular & cellular mechanisms22(4)360-375(2017 Apr)
Hosogane M,Bosu L,Fukumoto E,Yamada H,Sato S,Nakayama K

Characterization of conserved arginine residues on Cdt1 that affect licensing activity and interaction with Geminin or Mcm complex.
Cell cycle (Georgetown, Tex.)15(9)1213-26(2016 May)
You Z,Ode KL,Shindo M,Takisawa H,Masai H

The apical complex protein Pals1 is required to maintain cerebellar progenitor cells in a proliferative state.
Development (Cambridge, England)143(1)133-46(2016 Jan)
Park JY,Hughes LJ,Moon UY,Park R,Kim SB,Tran K,Lee JS,Cho SH,Kim S