Rras2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rras2
Entrez Gene ID	66922
Full Name	related RAS viral (r-ras) oncogene 2
Synonyms	2610016H24Rik,C86394,TC21
General protein information	Preferred Names related RAS viral (r-ras) oncogene 2 Names ras-related protein R-Ras2 related RAS viral (r-ras) oncogene homolog 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025846.2	NP_080122.2	ras-related protein R-Ras2
XM_017312239.1	XP_017167728.1	ras-related protein R-Ras2 isoform X1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RAS1	NP_014744.1
Danio rerio (zebrafish)	rras2	NP_001017815.1
Drosophila melanogaster (fruit fly)	Ras64B	NP_523917.2
Gallus gallus (chicken)	RRAS2	NP_001006466.1
Caenorhabditis elegans (roundworm)	ras-1	NP_496623.1
Saccharomyces cerevisiae (baker's yeast)	RAS2	NP_014301.1
Kluyveromyces lactis	KLLA0C13387g	XP_452797.2
Eremothecium gossypii	AGOS_ADL262W	NP_983834.2
Pan troglodytes (chimpanzee)	RRAS2	XP_003951891.1
	RRAS2	XP_002799673.1
Mus musculus (house mouse)	Rras2	NP_080122.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002812	XP_312101.5
Schizosaccharomyces pombe (fission yeast)	ras1	NP_593579.1
Magnaporthe oryzae (rice blast fungus)	MGG_06154	XP_003712033.1
Homo sapiens (human)	RRAS2	NP_036382.2
Canis lupus familiaris (dog)	RRAS2	XP_005633749.1
Bos taurus (cattle)	RRAS2	NP_001092480.1
Rattus norvegicus (Norway rat)	Rras2	NP_001013452.1
Neurospora crassa	NCU08823	XP_961863.2

Gene Ontology
Bibliography

Related articles in PubMed

SIRT6 regulates Ras-related protein R-Ras2 by lysine defatty-acylation.
Zhang X, Spiegelman NA, Nelson OD, Jing H, Lin H
eLife6(2017 04)

RLIP76 regulates Arf6-dependent cell spreading and migration by linking ARNO with activated R-Ras at recycling endosomes.
Wurtzel JG, Lee S, Singhal SS, Awasthi S, Ginsberg MH, Goldfinger LE
Biochemical and biophysical research communications467(4)785-91(2015 Nov)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

Contribution of the R-Ras2 GTP-binding protein to primary breast tumorigenesis and late-stage metastatic disease.
Larive RM, Moriggi G, Menacho-M?rquez M, Ca?amero M, de ?lava E, Alarc?n B, Dosil M, Bustelo XR
Nature communications53881(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SIRT6, a sirtuin with established tumor suppressor function, regulates the lysine fatty acylation of R-Ras2.
Title: SIRT6 regulates Ras-related protein R-Ras2 by lysine defatty-acylation.

The RLIP76/R-Ras/ARNO complex localizes to recycling endosomes.RLIP76 is a linker protein connecting R-Ras to ARNO.
Title: RLIP76 regulates Arf6-dependent cell spreading and migration by linking ARNO with activated R-Ras at recycling endosomes.

R-Ras2 has a role in both primary breast tumorigenesis and the late metastatic steps of cancer cells in the lung parenchyma.
Title: Contribution of the R-Ras2 GTP-binding protein to primary breast tumorigenesis and late-stage metastatic disease.

findings reveal R-Ras2 as a critical regulator of TGF-beta signaling in vivo.
Title: In vivo regulation of TGF-? by R-Ras2 revealed through loss of the RasGAP protein NF1.

Data indicate that RRas2(-/-) mice show no major alterations in viability, growth rates, cardiovascular parameters, or fertility.
Title: The Ras-like protein R-Ras2/TC21 is important for proper mammary gland development.

The following Rras2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rras2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01150	NM_025846.2 Latest version!	Mus musculus related RAS viral (r-ras) oncogene 2 (Rras2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu82190	XM_017312239.1 Latest version!	Mus musculus related RAS viral (r-ras) oncogene 2 (Rras2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rras2 ( NM_025846.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080122.2 Mus musculus related RAS viral (r-ras) oncogene 2 (Rras2), mRNA.

Price & Availability↑

CloneID	OMu01150
Clone ID Related Accession (Same CDS sequence)	NM_025846.2
Accession Version	NM_025846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	ras-related protein R-Ras2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK078870.1, AK011419.1, AK003733.1 and BX524069.1. On Jan 19, 2008 this sequence version replaced NM_025846.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK011419.1, SRR1660811.89655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCGG CCGGCTGGCG CGACGGCTCC GGCCAGGAGA AGTACCGGCT CGTGGTGGTC 
GGCGGTGGCG GCGTGGGCAA GTCGGCGCTC ACCATCCAGT TCATCCAGTC CTATTTTGTG 
ACGGACTATG ATCCAACCAT TGAAGACTCA TACACAAAGC AATGTGTGAT CGATGACCGA 
GCTGCCCGGC TGGACATTTT GGATACAGCC GGACAAGAGG AGTTTGGAGC CATGAGAGAG 
CAATACATGA GGACAGGCGA GGGCTTCCTG TTGGTCTTCT CAGTCACGGA TAGAGGCAGT 
TTTGAAGAAA TCTATAAGTT TCAAAGACAG ATTCTCAGAG TAAAGGACCG TGATGAGTTT 
CCCATGATTT TAATTGGCAA TAAAGCTGAC CTGGACCATC AGAGACAGGT AACACAGGAA 
GAAGGGCAGC AGTTAGCAAG GCAGCTCAAG GTCACATATA TGGAGGCATC GGCAAAGATC 
AGGATGAATG TAGACCAAGC TTTCCACGAA CTTGTCCGGG TTATAAGGAA ATTTCAAGAG 
CAAGAATGCC CTCCTTCACC AGAACCAACC CGGAAAGAAA AAGACAAGAA AGGCTGCCAT 
TGTGTCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080122.2
CDS	279..893
Translation	MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF

Target ORF information:

RefSeq Version	NM_025846.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus related RAS viral (r-ras) oncogene 2 (Rras2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025846.2

ORF Insert Sequence:

ATGGCCGCGG CCGGCTGGCG CGACGGCTCC GGCCAGGAGA AGTACCGGCT CGTGGTGGTC 
GGCGGTGGCG GCGTGGGCAA GTCGGCGCTC ACCATCCAGT TCATCCAGTC CTATTTTGTG 
ACGGACTATG ATCCAACCAT TGAAGACTCA TACACAAAGC AATGTGTGAT CGATGACCGA 
GCTGCCCGGC TGGACATTTT GGATACAGCC GGACAAGAGG AGTTTGGAGC CATGAGAGAG 
CAATACATGA GGACAGGCGA GGGCTTCCTG TTGGTCTTCT CAGTCACGGA TAGAGGCAGT 
TTTGAAGAAA TCTATAAGTT TCAAAGACAG ATTCTCAGAG TAAAGGACCG TGATGAGTTT 
CCCATGATTT TAATTGGCAA TAAAGCTGAC CTGGACCATC AGAGACAGGT AACACAGGAA 
GAAGGGCAGC AGTTAGCAAG GCAGCTCAAG GTCACATATA TGGAGGCATC GGCAAAGATC 
AGGATGAATG TAGACCAAGC TTTCCACGAA CTTGTCCGGG TTATAAGGAA ATTTCAAGAG 
CAAGAATGCC CTCCTTCACC AGAACCAACC CGGAAAGAAA AAGACAAGAA AGGCTGCCAT 
TGTGTCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rras2 ( XM_017312239.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167728.1 Mus musculus related RAS viral (r-ras) oncogene 2 (Rras2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82190
Clone ID Related Accession (Same CDS sequence)	XM_017312239.1
Accession Version	XM_017312239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras-related protein R-Ras2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGC AATACATGAG GACAGGCGAG GGCTTCCTGT TGGTCTTCTC AGTCACGGAT 
AGAGGCAGTT TTGAAGAAAT CTATAAGTTT CAAAGACAGA TTCTCAGAGT AAAGGACCGT 
GATGAGTTTC CCATGATTTT AATTGGCAAT AAAGCTGACC TGGACCATCA GAGACAGGTA 
ACACAGGAAG AAGGGCAGCA GTTAGCAAGG CAGCTCAAGG TCACATATAT GGAGGCATCG 
GCAAAGATCA GGATGAATGT AGACCAAGCT TTCCACGAAC TTGTCCGGGT TATAAGGAAA 
TTTCAAGAGC AAGAATGCCC TCCTTCACCA GAACCAACCC GGAAAGAAAA AGACAAGAAA 
GGCTGCCATT GTGTCATTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167728.1
CDS	1393..1776
Translation	MREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF

Target ORF information:

RefSeq Version	XM_017312239.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus related RAS viral (r-ras) oncogene 2 (Rras2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312239.1

ORF Insert Sequence:

ATGAGAGAGC AATACATGAG GACAGGCGAG GGCTTCCTGT TGGTCTTCTC AGTCACGGAT 
AGAGGCAGTT TTGAAGAAAT CTATAAGTTT CAAAGACAGA TTCTCAGAGT AAAGGACCGT 
GATGAGTTTC CCATGATTTT AATTGGCAAT AAAGCTGACC TGGACCATCA GAGACAGGTA 
ACACAGGAAG AAGGGCAGCA GTTAGCAAGG CAGCTCAAGG TCACATATAT GGAGGCATCG 
GCAAAGATCA GGATGAATGT AGACCAAGCT TTCCACGAAC TTGTCCGGGT TATAAGGAAA 
TTTCAAGAGC AAGAATGCCC TCCTTCACCA GAACCAACCC GGAAAGAAAA AGACAAGAAA 
GGCTGCCATT GTGTCATTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SIRT6 regulates Ras-related protein R-Ras2 by lysine defatty-acylation.
eLife6(2017 04)
Zhang X,Spiegelman NA,Nelson OD,Jing H,Lin H

RLIP76 regulates Arf6-dependent cell spreading and migration by linking ARNO with activated R-Ras at recycling endosomes.
Biochemical and biophysical research communications467(4)785-91(2015 Nov)
Wurtzel JG,Lee S,Singhal SS,Awasthi S,Ginsberg MH,Goldfinger LE

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

Contribution of the R-Ras2 GTP-binding protein to primary breast tumorigenesis and late-stage metastatic disease.
Nature communications53881(2014 May)
Larive RM,Moriggi G,Menacho-M?rquez M,Ca?amero M,de ?lava E,Alarc?n B,Dosil M,Bustelo XR