Defa42 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defa42
Entrez Gene ID	665927
Full Name	predicted gene 7849
Synonyms	EG665927
General protein information	Preferred Names predicted gene 7849 Names predicted gene, EG665927 novel defensin-related cryptidin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001177518.1	NP_001170989.1	predicted gene, EG665927 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	AY761184	NP_001007583.1
Mus musculus (house mouse)	Gm14851	NP_001170953.1
Mus musculus (house mouse)	Gm7861	NP_001170997.1
Mus musculus (house mouse)	Gm7849	NP_001170989.1
Mus musculus (house mouse)	Defa-rs1	NP_031870.2

Gene Ontology
Bibliography

Related articles in PubMed

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
Amid C, Rehaume LM, Brown KL, Gilbert JG, Dougan G, Hancock RE, Harrow JL
BMC genomics10606(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Defa42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defa42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00424	NM_001177518.1 Latest version!	Mus musculus defensin, alpha, 42 (Defa42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defa42 ( NM_001177518.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001170989.1 Mus musculus defensin, alpha, 42 (Defa42), mRNA.

Price & Availability↑

CloneID	OMu00424
Clone ID Related Accession (Same CDS sequence)	NM_001177518.1
Accession Version	NM_001177518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	predicted gene, EG665927 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC239604.3. On or before Apr 15, 2010 this sequence version replaced XM_909689.2, XM_980383.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACAC TTGTCCTCCT CTCTGCCCTT GCCCTGCTGG CCTTACAGGT CCAGGCTGAT 
CCTATCCAAA ACACAGATGA AGAGACTAAA ACTCAGGAGC AGCCAGGGGA AGAGGACCAG 
GCTGTTTCTG TCTCCTTTGG AGGCACAGAA GGCTCTGCTC TTCAAGATGT AGCCCAAAGA 
AGATTTCCGT GGTGCCGGAA GTGCCGAGTG TGCCAGAAGT GCGAAGTGTG CCAGAAGTGC 
CCTGTGTGCC CGACATGCCC CCAGTGCCCA AAGCAGCCAT TGTGCAAAGA AAGGCAAAAT 
AAAACTGCTA TCACCACCCA AGCTCCAAAT ACACATCATA AAGGCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001170989.1
CDS	1..351
Translation	MKTLVLLSALALLALQVQADPIQNTDEETKTQEQPGEEDQAVSVSFGGTEGSALQDVAQRRFPWCRKCRVCQKCEVCQKCPVCPTCPQCPKQPLCKERQNKTAITTQAPNTHHKGC

Target ORF information:

RefSeq Version	NM_001177518.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus defensin, alpha, 42 (Defa42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177518.1

ORF Insert Sequence:

ATGAAGACAC TTGTCCTCCT CTCTGCCCTT GCCCTGCTGG CCTTACAGGT CCAGGCTGAT 
CCTATCCAAA ACACAGATGA AGAGACTAAA ACTCAGGAGC AGCCAGGGGA AGAGGACCAG 
GCTGTTTCTG TCTCCTTTGG AGGCACAGAA GGCTCTGCTC TTCAAGATGT AGCCCAAAGA 
AGATTTCCGT GGTGCCGGAA GTGCCGAGTG TGCCAGAAGT GCGAAGTGTG CCAGAAGTGC 
CCTGTGTGCC CGACATGCCC CCAGTGCCCA AAGCAGCCAT TGTGCAAAGA AAGGCAAAAT 
AAAACTGCTA TCACCACCCA AGCTCCAAAT ACACATCATA AAGGCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
BMC genomics10606(2009 Dec)
Amid C,Rehaume LM,Brown KL,Gilbert JG,Dougan G,Hancock RE,Harrow JL