Mrps25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrps25
Entrez Gene ID	64658
Full Name	mitochondrial ribosomal protein S25
Synonyms	2810429N01Rik,Rpms25
General protein information	Preferred Names mitochondrial ribosomal protein S25 Names 28S ribosomal protein S25, mitochondrial MRP-S25 S25mt
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025578.4	NP_079854.2	28S ribosomal protein S25, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	MRPS25	NP_071942.1
	MRPS25	XP_001091560.2
Caenorhabditis elegans (roundworm)	mrps-25	NP_500032.1
Danio rerio (zebrafish)	mrps25	NP_001017590.1
Canis lupus familiaris (dog)	MRPS25	XP_533729.3
Rattus norvegicus (Norway rat)	Mrps25	NP_001020579.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012136	XP_320391.3
Xenopus tropicalis (tropical clawed frog)	LOC100494871	XP_002938273.1
Pan troglodytes (chimpanzee)	MRPS25	NP_001238852.1
Bos taurus (cattle)	MRPS25	NP_001069447.1
Mus musculus (house mouse)	Mrps25	NP_079854.2
Gallus gallus (chicken)	MRPS25	NP_001264769.1
Drosophila melanogaster (fruit fly)	mRpS25	NP_511153.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Kitamura H, Nakagawa T, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mrps25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrps25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13467	NM_025578.4 Latest version!	Mus musculus mitochondrial ribosomal protein S25 (Mrps25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrps25 ( NM_025578.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079854.2 Mus musculus mitochondrial ribosomal protein S25 (Mrps25), mRNA.

Price & Availability↑

CloneID	OMu13467
Clone ID Related Accession (Same CDS sequence)	NM_025578.4
Accession Version	NM_025578.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	28S ribosomal protein S25, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164642.3. On Nov 17, 2006 this sequence version replaced NM_025578.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK131114.1, BC022953.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCCGATGA AGGGTCGCTT CCCGATCCGC CGTACGCTGC AGTACCTGGG TCGCGGGGAC 
GTGGTATTCA AGGAGTCTGT GAAGATCATG ACCGTGAACT ACAACACGTA TGGGGAACTG 
GGCGAAGGCG CCAGGAAATT TGTGTTTTTC AACATCCCTC AGATCCAGTA TAAGAATCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCATCGC CCTTCCTGCG GTTCTATCTA 
GATTCTGGGG AGCAAGTCCT GGTAGACGTG GAGACGAAGA GTAATAAGGA GATCATGGAG 
CACATAAAGA AAATCTTGGG CAAGAAAGAG GAGACCCTCA GGGAGGAGGA GCTGGAAAAG 
CAGCAGCGCT TTCATCCAGG CAACTTTGGC CCCCGGAAGT ACTGCCTGCG GGAGTGCATG 
TGCGAAGTAG AAGGCCAGGT GCCCTGCCCT GGCTTGGTGC CCTTACCCAA GGAGATGACA 
GGGAAGTACA AAGCTGCTCT GAAAGCCAGT ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079854.2
CDS	51..566
Translation	MPMKGRFPIRRTLQYLGRGDVVFKESVKIMTVNYNTYGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNMTPSPFLRFYLDSGEQVLVDVETKSNKEIMEHIKKILGKKEETLREEELEKQQRFHPGNFGPRKYCLRECMCEVEGQVPCPGLVPLPKEMTGKYKAALKAST

Target ORF information:

RefSeq Version	NM_025578.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein S25 (Mrps25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025578.4

ORF Insert Sequence:

ATGCCGATGA AGGGTCGCTT CCCGATCCGC CGTACGCTGC AGTACCTGGG TCGCGGGGAC 
GTGGTATTCA AGGAGTCTGT GAAGATCATG ACCGTGAACT ACAACACGTA TGGGGAACTG 
GGCGAAGGCG CCAGGAAATT TGTGTTTTTC AACATCCCTC AGATCCAGTA TAAGAATCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCATCGC CCTTCCTGCG GTTCTATCTA 
GATTCTGGGG AGCAAGTCCT GGTAGACGTG GAGACGAAGA GTAATAAGGA GATCATGGAG 
CACATAAAGA AAATCTTGGG CAAGAAAGAG GAGACCCTCA GGGAGGAGGA GCTGGAAAAG 
CAGCAGCGCT TTCATCCAGG CAACTTTGGC CCCCGGAAGT ACTGCCTGCG GGAGTGCATG 
TGCGAAGTAG AAGGCCAGGT GCCCTGCCCT GGCTTGGTGC CCTTACCCAA GGAGATGACA 
GGGAAGTACA AAGCTGCTCT GAAAGCCAGT ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Kitamura H,Nakagawa T,Nagase T,Ohara O,Koga H

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M