Psme2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psme2b
Entrez Gene ID	621823
Full Name	protease (prosome, macropain) activator subunit 2B
Synonyms	PA28b-p,PA28b2,Psme2-like,Psme2b-ps
General protein information	Preferred Names protease (prosome, macropain) activator subunit 2B Names protease (prosome, macropain) 28 subunit beta B OTTMUSWSBG00059279 OTTMUSWSBP00075795 PA28 beta subunit (PA28b) pseudogene protease (prosome, macropain) 28 subunit beta B, pseudogene protease (prosome, macropain) 28 subunit, beta, b proteasome activator complex like
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.2
Summary	This gene may encode proteasome activator complex subunit 2 protein (PMID: 10222192). This gene, located on chromosome 11, lacks a structured promoter and is intronless. It has also been described as a pseudogene (PMID: 9914329), however, it has an intact coding region and transcripts can be placed at this location unambiguously. An interferon-inducible gene located on chromosome 14, near the gene that encodes proteasome activator complex subunit 1 protein, is considered the functional gene. [provided by RefSeq, Jul 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281472.1	NP_001268401.1	protease (prosome, macropain) 28 subunit beta B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transposable elements and viruses as factors in adaptation and evolution: an expansion and strengthening of the TE-Thrust hypothesis.
Oliver KR, Greene WK
Ecology and evolution2(11)2912-33(2012 Nov)

Differential expression regulation of the alpha and beta subunits of the PA28 proteasome activator in mature dendritic cells.
Ossendorp F, Fu N, Camps M, Granucci F, Gobin SJ, van den Elsen PJ, Schuurhuis D, Adema GJ, Lipford GB, Chiba T, Sijts A, Kloetzel PM, Ricciardi-Castagnoli P, Melief CJ
Journal of immunology (Baltimore, Md. : 1950)174(12)7815-22(2005 Jun)

Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta.
Murata S, Udono H, Tanahashi N, Hamada N, Watanabe K, Adachi K, Yamano T, Yui K, Kobayashi N, Kasahara M, Tanaka K, Chiba T
The EMBO journal20(21)5898-907(2001 Nov)

Nucleotide sequence analysis of the approximately 35-kb segment containing interferon-gamma-inducible mouse proteasome activator genes.
Yawata M, Murata S, Tanaka K, Ishigatsubo Y, Kasahara M
Immunogenetics53(2)119-29(2001 Mar)

A second gene encoding the mouse proteasome activator PA28beta subunit is part of a LINE1 element and is driven by a LINE1 promoter.
Zaiss DM, Kloetzel PM
Journal of molecular biology287(5)829-35(1999 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Induction of PA28 beta expression allows proper PA28 alpha beta complex formation, thereby enhancing proteasome activity in activated dendritic cells.
Title: Differential expression regulation of the alpha and beta subunits of the PA28 proteasome activator in mature dendritic cells.

MEF from mice deficient for PA28a and PA28b do not have detectable mRNA and protein for these proteins even after gamma interferon stimulation, suggesting only one functional copy of PA28a and PA28b.
Title: Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta.

Psme2b is an expressed retrotransposon. Unlike Psme2, Psme2b is constitutive not cytokine-inducible, as a result of insertion into a transcriptionally active LINE1 element.
Title: A second gene encoding the mouse proteasome activator PA28beta subunit is part of a LINE1 element and is driven by a LINE1 promoter.

Psme2b is intronless and lacks a structured promoter so appears to be a processed Psme2 pseudogene.
Title: Characterization of the mouse proteasome regulator PA28b gene.

The following Psme2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psme2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu57634	NM_001281472.1 Latest version!	Mus musculus protease (prosome, macropain) activator subunit 2B (Psme2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psme2b ( NM_001281472.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268401.1 Mus musculus protease (prosome, macropain) activator subunit 2B (Psme2b), mRNA.

Price & Availability↑

CloneID	OMu57634
Clone ID Related Accession (Same CDS sequence)	NM_001281472.1
Accession Version	NM_001281472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-25
Organism	Mus musculus(house mouse)
Product	protease (prosome, macropain) 28 subunit beta B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE624310.1, D87910.1 and BB844325.1.

ATGGCCAAGC CTTGTGGGGT CCGCTTGAGT GGAGAAGCCC GAAAACAGGT GGATGTCTTC 
AGACAAAATC TTTTCCAGGA GGCTGATGAC TTCCTCTGCA CTTTCTTGCC ACGGAAAATC 
ATATCCCTGA GTCAGCTCTT GCAGGAGGAT TCCCTCAATG TGGCCGACCT CTCCTCCCTC 
CGGGCTCCTC TGGACATCCC TATCCCAGAT CCTCCACCCA AGGATGACGA GATGGAAACA 
GACAAGCAGG AGAAGAAGGA AGTCCCTAAG TGTGGCTACC TCCCGGGGAA CGAGAAGCTT 
CTGGCCTTGC TTGCTTTGGT TAAGCCAGAA GTCTGGACTC TCAAAGAGAA GTGCATTCTG 
GTAATCACGT GGATCCAGCA CCTGATCCCC AAGATTGAGG ATGGAAATGA TTTTGGGGTG 
GCAATTCAGG AGAAGGTGCT GGAGAGAGTG AATGCTGTCA AGACCAAAGT AGAAGCTTTC 
CAGACAACTA TTTCCAAGTA CTTCTCAGAA CGAGGGGATG CTGTAGCCAA GGCTTCCAAG 
GATACCCATG TAATGGATTA CCGGGCCTTG GTGCATGAGC GAGATGAAGC AGCCTATGGG 
GCGCTTAGGG CCATGGTGCT GGACCTGAGG GCCTTCTATG CTGAGCTTTA TCATATCATC 
AGCAGCAACC TAGAGAAAAT CGTCAACCCG AAGGGTGAAG AAAAGCCATC GATGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268401.1
CDS	293..1012
Translation	MAKPCGVRLSGEARKQVDVFRQNLFQEADDFLCTFLPRKIISLSQLLQEDSLNVADLSSLRAPLDIPIPDPPPKDDEMETDKQEKKEVPKCGYLPGNEKLLALLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGVAIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKDTHVMDYRALVHERDEAAYGALRAMVLDLRAFYAELYHIISSNLEKIVNPKGEEKPSMY

Target ORF information:

RefSeq Version	NM_001281472.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease (prosome, macropain) activator subunit 2B (Psme2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281472.1

ORF Insert Sequence:

ATGGCCAAGC CTTGTGGGGT CCGCTTGAGT GGAGAAGCCC GAAAACAGGT GGATGTCTTC 
AGACAAAATC TTTTCCAGGA GGCTGATGAC TTCCTCTGCA CTTTCTTGCC ACGGAAAATC 
ATATCCCTGA GTCAGCTCTT GCAGGAGGAT TCCCTCAATG TGGCCGACCT CTCCTCCCTC 
CGGGCTCCTC TGGACATCCC TATCCCAGAT CCTCCACCCA AGGATGACGA GATGGAAACA 
GACAAGCAGG AGAAGAAGGA AGTCCCTAAG TGTGGCTACC TCCCGGGGAA CGAGAAGCTT 
CTGGCCTTGC TTGCTTTGGT TAAGCCAGAA GTCTGGACTC TCAAAGAGAA GTGCATTCTG 
GTAATCACGT GGATCCAGCA CCTGATCCCC AAGATTGAGG ATGGAAATGA TTTTGGGGTG 
GCAATTCAGG AGAAGGTGCT GGAGAGAGTG AATGCTGTCA AGACCAAAGT AGAAGCTTTC 
CAGACAACTA TTTCCAAGTA CTTCTCAGAA CGAGGGGATG CTGTAGCCAA GGCTTCCAAG 
GATACCCATG TAATGGATTA CCGGGCCTTG GTGCATGAGC GAGATGAAGC AGCCTATGGG 
GCGCTTAGGG CCATGGTGCT GGACCTGAGG GCCTTCTATG CTGAGCTTTA TCATATCATC 
AGCAGCAACC TAGAGAAAAT CGTCAACCCG AAGGGTGAAG AAAAGCCATC GATGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transposable elements and viruses as factors in adaptation and evolution: an expansion and strengthening of the TE-Thrust hypothesis.
Ecology and evolution2(11)2912-33(2012 Nov)
Oliver KR,Greene WK

Differential expression regulation of the alpha and beta subunits of the PA28 proteasome activator in mature dendritic cells.
Journal of immunology (Baltimore, Md. : 1950)174(12)7815-22(2005 Jun)
Ossendorp F,Fu N,Camps M,Granucci F,Gobin SJ,van den Elsen PJ,Schuurhuis D,Adema GJ,Lipford GB,Chiba T,Sijts A,Kloetzel PM,Ricciardi-Castagnoli P,Melief CJ

Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta.
The EMBO journal20(21)5898-907(2001 Nov)
Murata S,Udono H,Tanahashi N,Hamada N,Watanabe K,Adachi K,Yamano T,Yui K,Kobayashi N,Kasahara M,Tanaka K,Chiba T

Nucleotide sequence analysis of the approximately 35-kb segment containing interferon-gamma-inducible mouse proteasome activator genes.
Immunogenetics53(2)119-29(2001 Mar)
Yawata M,Murata S,Tanaka K,Ishigatsubo Y,Kasahara M

A second gene encoding the mouse proteasome activator PA28beta subunit is part of a LINE1 element and is driven by a LINE1 promoter.
Journal of molecular biology287(5)829-35(1999 Apr)
Zaiss DM,Kloetzel PM