Hacl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hacl1
Entrez Gene ID	56794
Full Name	2-hydroxyacyl-CoA lyase 1
Synonyms	1600020H07Rik,Hpcl,Phyh2
General protein information	Preferred Names 2-hydroxyacyl-CoA lyase 1 Names 2-hydroxyacyl-CoA lyase 1 2-HPCL 2-hydroxyphytanoyl-CoA lyase phytanoyl-CoA 2-hydroxylase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_019975.3	NP_064359.2	2-hydroxyacyl-CoA lyase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HACL1	XP_001160528.1
Drosophila melanogaster (fruit fly)	CG11208	NP_611460.1
Schizosaccharomyces pombe (fission yeast)	SPBC725.04	NP_595484.1
Homo sapiens (human)	HACL1	NP_036392.2
Bos taurus (cattle)	HACL1	NP_001092419.1
Danio rerio (zebrafish)	hacl1	NP_998250.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010368	XP_311576.3
Eremothecium gossypii	AGOS_AEL055C	NP_984806.1
Gallus gallus (chicken)	HACL1	NP_001186557.1
Arabidopsis thaliana (thale cress)	AT5G17380	NP_197240.1
Saccharomyces cerevisiae (baker's yeast)	YEL020C	NP_010895.1
Xenopus tropicalis (tropical clawed frog)	hacl1	NP_001017332.1
Canis lupus familiaris (dog)	HACL1	XP_534256.4
Mus musculus (house mouse)	Hacl1	NP_064359.2
Rattus norvegicus (Norway rat)	Hacl1	NP_445945.1
Caenorhabditis elegans (roundworm)	CELE_B0334.3	NP_496454.1

Gene Ontology
Bibliography

Related articles in PubMed

Peroxisomal 2-Hydroxyacyl-CoA Lyase Is Involved in Endogenous Biosynthesis of Heptadecanoic Acid.
Jenkins B, de Schryver E, Van Veldhoven PP, Koulman A
Molecules (Basel, Switzerland)22(10)(2017 10)

RP-HPLC-fluorescence analysis of aliphatic aldehydes: application to aldehyde-generating enzymes HACL1 and SGPL1.
Mezzar S, de Schryver E, Van Veldhoven PP
Journal of lipid research55(3)573-82(2014 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS.
Lee J, Xu Y, Chen Y, Sprung R, Kim SC, Xie S, Zhao Y
Molecular & cellular proteomics : MCP6(4)669-76(2007 Apr)

Breakdown of 2-hydroxylated straight chain fatty acids via peroxisomal 2-hydroxyphytanoyl-CoA lyase: a revised pathway for the alpha-oxidation of straight chain fatty acids.
Foulon V, Sniekers M, Huysmans E, Asselberghs S, Mahieu V, Mannaerts GP, Van Veldhoven PP, Casteels M
The Journal of biological chemistry280(11)9802-12(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

2-hydroxyphytanoyl-CoA lyase has a role in the breakdown of long chain 2-hydroxyfatty acids
Title: Breakdown of 2-hydroxylated straight chain fatty acids via peroxisomal 2-hydroxyphytanoyl-CoA lyase: a revised pathway for the alpha-oxidation of straight chain fatty acids.

The following Hacl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hacl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15368	NM_019975.3 Latest version!	Mus musculus 2-hydroxyacyl-CoA lyase 1 (Hacl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hacl1 ( NM_019975.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064359.2 Mus musculus 2-hydroxyacyl-CoA lyase 1 (Hacl1), mRNA.

Price & Availability↑

CloneID	OMu15368
Clone ID Related Accession (Same CDS sequence)	NM_019975.3
Accession Version	NM_019975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	2-hydroxyacyl-CoA lyase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY340097.1 and AK041686.1. On Dec 9, 2009 this sequence version replaced NM_019975.2. ##Evidence-Data-START## Transcript exon combination :: AK041686.1, AJ132139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGCCAGAAA GTAACTCCGC AGAGGGTAGC GACAGAAGCG AGGAGCAGGT GTCTGGTGCT 
AAAGTCATCG CCCAGGCTCT AAAAACTCAA GATGTGGAGT ACATGTTCGG TGTCGTAGGC 
ATCCCCGTGA CCGAAATCGC CCTTGCTGCC CAGGAACTGG GCATCAAGTA CATTGGGATG 
CGGAATGAGC AAGCGGCTTG TTATGCTGCC TCTGCAGTTG GATACCTGAC AGGCAGGCCA 
GGAGTGTGCC TTGTTGTTTC TGGTCCCGGT CTCATCCACG CCTTGGGCGG CATGGCAAAT 
GCAAACATGA ACTGTTGGCC CTTGATTGTG ATTGGTGGTT CTTCTGAAAG AAATCAAGAA 
GCTATGGGAG CCTTCCAGGA GTTTCCACAG GTTGAAGCTT GCAGATTGTA TACCAAGTTC 
TCGGCCCGGC CAAGCACCAT AGAGCTCATT CCGTTTATTA TTGAAAAGGC AGTGAGGAGC 
AGTATCTATG GTCGCCCGGG AGCTTGTTAC ATTGACATTC CTGCAGATTT TGTGACCCTC 
CAGGCGAACG TGACTTCTAT AAAATACAAG GAGTGCTGCA TGCCACCTCC AGTTAGCATG 
GCAGAAACCT CTGCTGTGTG CGCAGCAGCC TCTGTTCTTC GGGATGCCAA GCAACCCCTG 
CTTATCATCG GGAAAGGCGC TGCCTATTCC CACGCAGAGG ACAGCATCAG GAAGCTGGTG 
GAGCAGTGCA GTTTGCCATT TTTGCCTACG CCTATGGGGA AGGGGGTTGT CCCTGACAAT 
CATCCCAACT GTGTAGGCGC GGCCAGATCC AGGGCTTTGC AGTCCGCTGA CGTGATTGTG 
TTATTTGGTG CGAGACTAAA CTGGATCTTA CATTTTGGAC TGCCTCCAAG ATACCAGGCA 
GACGTGAAGT TTATTCAGAT TGATATCTGT GCAGAAGAAC TGGGGAATAA TGTCAGGCCT 
TCTGTGATTT TGCTAGGAGA TATAGATGCT GTCTCTAAAC AGCTTTTAGA ACAGTTTGAT 
AAAACTCCGT GGCAGTGCCC CCCAGACAGC CAGTGGTGGA AGACTCTGAG GGAGAAAATG 
AAGAGCAATG AAGCCATCTC CAAGGAACTG GCTTCCCAAA AGTCCCTCCC TATGAATTAT 
TACACAGTGT TCTACCACGT TCAAGAACAG TTGCCCCGAG ACTCTTTCAT AGTGAGTGAG 
GGGGCAAACA CCATGGACAT CGGACGGACT ATGCTTCAGA ACTGCCTTCC TCGCCACAGG 
CTTGATGCAG GTTCTTTTGG AACAATGGGG GTTGGCCTGG GATTTGCCAT TGCAGCCGCT 
CTAGTGGCTA AAGACAGAAG TCCTGGGCAG CGGGTCATCT GTGTGGAAGG TGACAGTGCC 
TTCGGATTTT CTGGCATGGA AGTGGAGACC ATCTGCAGGT ACAACTTGCC AATCATACTC 
TTGGTAGTAA ACAATAATGG AATTTACCAA GGTTTTGACG CTGACACCTG GGAAAAAATG 
CTACATTTTC AAGAAGCTGC TACAACTGTC CCTCCAATGT GCCTCTTGCC AAACTCGCAT 
TATGAGCAGG TCATGACTGC ATTTGGAGGC AAAGGGTATT TTGTACGAAC ACCAGAAGAG 
CTCCAACACT CGCTGAGGCA GGCTCTGCAA GACACAAGTA AACCCTGCCT TCTCAACATT 
ATGATTGAGC CACAAAGTAC GCGCAAGGCC CAGGATTTTC ACTGGCTCAC CCGTTCTAAC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064359.2
CDS	88..1833
Translation	MPESNSAEGSDRSEEQVSGAKVIAQALKTQDVEYMFGVVGIPVTEIALAAQELGIKYIGMRNEQAACYAASAVGYLTGRPGVCLVVSGPGLIHALGGMANANMNCWPLIVIGGSSERNQEAMGAFQEFPQVEACRLYTKFSARPSTIELIPFIIEKAVRSSIYGRPGACYIDIPADFVTLQANVTSIKYKECCMPPPVSMAETSAVCAAASVLRDAKQPLLIIGKGAAYSHAEDSIRKLVEQCSLPFLPTPMGKGVVPDNHPNCVGAARSRALQSADVIVLFGARLNWILHFGLPPRYQADVKFIQIDICAEELGNNVRPSVILLGDIDAVSKQLLEQFDKTPWQCPPDSQWWKTLREKMKSNEAISKELASQKSLPMNYYTVFYHVQEQLPRDSFIVSEGANTMDIGRTMLQNCLPRHRLDAGSFGTMGVGLGFAIAAALVAKDRSPGQRVICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDADTWEKMLHFQEAATTVPPMCLLPNSHYEQVMTAFGGKGYFVRTPEELQHSLRQALQDTSKPCLLNIMIEPQSTRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_019975.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 2-hydroxyacyl-CoA lyase 1 (Hacl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019975.3

ORF Insert Sequence:

ATGCCAGAAA GTAACTCCGC AGAGGGTAGC GACAGAAGCG AGGAGCAGGT GTCTGGTGCT 
AAAGTCATCG CCCAGGCTCT AAAAACTCAA GATGTGGAGT ACATGTTCGG TGTCGTAGGC 
ATCCCCGTGA CCGAAATCGC CCTTGCTGCC CAGGAACTGG GCATCAAGTA CATTGGGATG 
CGGAATGAGC AAGCGGCTTG TTATGCTGCC TCTGCAGTTG GATACCTGAC AGGCAGGCCA 
GGAGTGTGCC TTGTTGTTTC TGGTCCCGGT CTCATCCACG CCTTGGGCGG CATGGCAAAT 
GCAAACATGA ACTGTTGGCC CTTGATTGTG ATTGGTGGTT CTTCTGAAAG AAATCAAGAA 
GCTATGGGAG CCTTCCAGGA GTTTCCACAG GTTGAAGCTT GCAGATTGTA TACCAAGTTC 
TCGGCCCGGC CAAGCACCAT AGAGCTCATT CCGTTTATTA TTGAAAAGGC AGTGAGGAGC 
AGTATCTATG GTCGCCCGGG AGCTTGTTAC ATTGACATTC CTGCAGATTT TGTGACCCTC 
CAGGCGAACG TGACTTCTAT AAAATACAAG GAGTGCTGCA TGCCACCTCC AGTTAGCATG 
GCAGAAACCT CTGCTGTGTG CGCAGCAGCC TCTGTTCTTC GGGATGCCAA GCAACCCCTG 
CTTATCATCG GGAAAGGCGC TGCCTATTCC CACGCAGAGG ACAGCATCAG GAAGCTGGTG 
GAGCAGTGCA GTTTGCCATT TTTGCCTACG CCTATGGGGA AGGGGGTTGT CCCTGACAAT 
CATCCCAACT GTGTAGGCGC GGCCAGATCC AGGGCTTTGC AGTCCGCTGA CGTGATTGTG 
TTATTTGGTG CGAGACTAAA CTGGATCTTA CATTTTGGAC TGCCTCCAAG ATACCAGGCA 
GACGTGAAGT TTATTCAGAT TGATATCTGT GCAGAAGAAC TGGGGAATAA TGTCAGGCCT 
TCTGTGATTT TGCTAGGAGA TATAGATGCT GTCTCTAAAC AGCTTTTAGA ACAGTTTGAT 
AAAACTCCGT GGCAGTGCCC CCCAGACAGC CAGTGGTGGA AGACTCTGAG GGAGAAAATG 
AAGAGCAATG AAGCCATCTC CAAGGAACTG GCTTCCCAAA AGTCCCTCCC TATGAATTAT 
TACACAGTGT TCTACCACGT TCAAGAACAG TTGCCCCGAG ACTCTTTCAT AGTGAGTGAG 
GGGGCAAACA CCATGGACAT CGGACGGACT ATGCTTCAGA ACTGCCTTCC TCGCCACAGG 
CTTGATGCAG GTTCTTTTGG AACAATGGGG GTTGGCCTGG GATTTGCCAT TGCAGCCGCT 
CTAGTGGCTA AAGACAGAAG TCCTGGGCAG CGGGTCATCT GTGTGGAAGG TGACAGTGCC 
TTCGGATTTT CTGGCATGGA AGTGGAGACC ATCTGCAGGT ACAACTTGCC AATCATACTC 
TTGGTAGTAA ACAATAATGG AATTTACCAA GGTTTTGACG CTGACACCTG GGAAAAAATG 
CTACATTTTC AAGAAGCTGC TACAACTGTC CCTCCAATGT GCCTCTTGCC AAACTCGCAT 
TATGAGCAGG TCATGACTGC ATTTGGAGGC AAAGGGTATT TTGTACGAAC ACCAGAAGAG 
CTCCAACACT CGCTGAGGCA GGCTCTGCAA GACACAAGTA AACCCTGCCT TCTCAACATT 
ATGATTGAGC CACAAAGTAC GCGCAAGGCC CAGGATTTTC ACTGGCTCAC CCGTTCTAAC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Peroxisomal 2-Hydroxyacyl-CoA Lyase Is Involved in Endogenous Biosynthesis of Heptadecanoic Acid.
Molecules (Basel, Switzerland)22(10)(2017 10)
Jenkins B,de Schryver E,Van Veldhoven PP,Koulman A

RP-HPLC-fluorescence analysis of aliphatic aldehydes: application to aldehyde-generating enzymes HACL1 and SGPL1.
Journal of lipid research55(3)573-82(2014 Mar)
Mezzar S,de Schryver E,Van Veldhoven PP

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS.
Molecular & cellular proteomics : MCP6(4)669-76(2007 Apr)
Lee J,Xu Y,Chen Y,Sprung R,Kim SC,Xie S,Zhao Y

Breakdown of 2-hydroxylated straight chain fatty acids via peroxisomal 2-hydroxyphytanoyl-CoA lyase: a revised pathway for the alpha-oxidation of straight chain fatty acids.
The Journal of biological chemistry280(11)9802-12(2005 Mar)
Foulon V,Sniekers M,Huysmans E,Asselberghs S,Mahieu V,Mannaerts GP,Van Veldhoven PP,Casteels M