Alg10b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alg10b
Entrez Gene ID	380959
Full Name	asparagine-linked glycosylation 10B (alpha-1,2-glucosyltransferase)
Synonyms	AA469671,AU045404,AW123895,C85569,Deaf1,Gm917,nse5
General protein information	Preferred Names asparagine-linked glycosylation 10B (alpha-1,2-glucosyltransferase) Names putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase alpha-1,2-glucosyltransferase ALG10-A alpha-2-glucosyltransferase ALG10-B asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase) asparagine-linked glycosylation protein 10 homolog B potassium channel regulator 1 putative alpha-1,2-glucosyltransferase ALG10-B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001033441.3	NP_001028613.1	putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E15797g	XP_454662.1
Arabidopsis thaliana (thale cress)	AT5G02410	NP_195861.2
Pan troglodytes (chimpanzee)	ALG10	XP_003313875.1
Mus musculus (house mouse)	Alg10b	NP_001028613.1
Rattus norvegicus (Norway rat)	Alg10	NP_620801.1
Gallus gallus (chicken)	ALG10	NP_001026565.1
Danio rerio (zebrafish)	LOC100331575	XP_002661512.1
Drosophila melanogaster (fruit fly)	Alg10	NP_729680.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002420	XP_312525.4
Schizosaccharomyces pombe (fission yeast)	alg10	NP_593221.1
Xenopus tropicalis (tropical clawed frog)	alg10	NP_001016192.2
Homo sapiens (human)	ALG10	NP_116223.3
Canis lupus familiaris (dog)	ALG10	XP_534842.3
Bos taurus (cattle)	ALG10	NP_001069657.2
Saccharomyces cerevisiae (baker's yeast)	DIE2	NP_011743.2
Eremothecium gossypii	AGOS_ADL138C	NP_983958.2
Oryza sativa (rice)	Os04g0503700	NP_001053242.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b) causes nonsyndromic hearing impairment in mice (Mus musculus).
Probst FJ, Corrigan RR, Del Gaudio D, Salinger AP, Lorenzo I, Gao SS, Chiu I, Xia A, Oghalai JS, Justice MJ
PloS one8(11)e80408(2013)

Functional genetic analysis of mouse chromosome 11.
Kile BT, Hentges KE, Clark AT, Nakamura H, Salinger AP, Liu B, Box N, Stockton DW, Johnson RL, Behringer RR, Bradley A, Justice MJ
Nature425(6953)81-6(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b) causes nonsyndromic hearing impairment in mice.
Title: A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b) causes nonsyndromic hearing impairment in mice (Mus musculus).

The following Alg10b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alg10b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12678	NM_001033441.3 Latest version!	Mus musculus asparagine-linked glycosylation 10B (alpha-1,2-glucosyltransferase) (Alg10b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alg10b ( NM_001033441.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028613.1 Mus musculus asparagine-linked glycosylation 10B (alpha-1,2-glucosyltransferase) (Alg10b), mRNA.

Price & Availability↑

CloneID	OMu12678
Clone ID Related Accession (Same CDS sequence)	NM_001033441.3
Accession Version	NM_001033441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC158922.8. On May 2, 2007 this sequence version replaced NM_001033441.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK147821.1, AK170450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCAGC TGGAGGGCTA TTACTTCTCG GCCGCCTTGA GCTGCACCTT CCTGGTGTCC 
TGCCTGCTCT TCTCCGCCTT CAGCCGCGCT CTGCGGGAGC CGTACATGGA CGAGATCTTC 
CACCTGCCGC AGGCGCAGCG GTACTGCGAG GGCCGCTTCT CCCTGTCGCA GTGGGATCCT 
ATGATCACTA CATTACCTGG CCTGTATCTG GTGTCGGTCG GTGTGGTCAA ACCTGCCAGC 
TGGCTCCTTG GATGGTCTGA GCATGTGATC TGCTCCATTG GGGTGCTCAG ATTTGTCAAT 
CTTCTCTTCA GCGTTGGCAA CTTCTACTTG CTGTATTTGC TCTTCAGGAA GGTGCAACCC 
AGAAACAAGG CTTCTTCAAG TATCCAGAGA ATCTTGTCAA CATTAACCCT AGCAGTGTTT 
CCAACCCTCT ATTTTTTTAA CTTCCTTTAC TATACAGAAG CTGGGTCTGT ATTCTTCACT 
CTTTTTGCTT ATTTGATGTG TCTTTATGGC AACCATAGGA CCTCGGCCTT GCTTGGATTT 
TGTGGTTTCA TGTTTCGTCA GACCAACATC ATCTGGGCGG CCTTCTGTGC AGGACACCTC 
ATTGCACAGA AGTGCAGTGA AGCCTGGAAG ATTGAGCTGC AGAAGAAGAA GGAGGAGAGG 
CTTGCGCCCA CGAAGGGACC GCTCTCGGAG CTCAGAAGAG TGCTGCAGTT TCTGCTGGTG 
TACGCCATGT CCCTAAAGAA CCTGAGAATG CTCTTCCTGT TGACCTGGCC CTACGTGCTT 
CTGCTGTTGG CGTTCTTTGC CTTTGTGGTA GTTAATGGTG GCATTGTTGT TGGTGACCGG 
AGTAGTCACG AGGCCTGTCT CCATTTTCCT CAGTTGTTCT ATTTCTTCTC CTTTACTGCC 
TTTTTCTCCT TCCCTCACCT ACTTTCTCTG ACCAAAGTCA AGACTTTCCT TAGCTTAGTT 
TGGAAGCGTA GAGTCCAGTT CTCTGTGGTT ACGTTAGTCT CGATACTTTT GGTTTGGAAA 
TTCACTTATG TCCATAAGTA TTTACTGGCA GACAATAGGC ATTACACATT TTATGTGTGG 
AAAAGAGTAT TTCAGAGACA TGAAGTTGTC AAATATTTAT TAGTTCCAGC CTACATTTTT 
GCTGGTTGGG CTATAGCTGA CTCTTTAAAG GCTAAGTCAA TTTTCTGGAA TTTAATGTTT 
TTTGTATGCT TGGTTGCTTC TACAGTTCCT CAGAAACTAC TAGAATTCCG TTACTTCATT 
CTACCATACA TTATTTATAG GCTTAACATA CCTCTGCCAC CCATATCTAG ACTCGTTTGT 
GAACTGGGTT GCTATACAGT TGTTAATTTT GTAACTTTTT ATATCTTTCT GAACAAGACT 
TTTCAGTGGC CAAATAGTCA GGACATCCAA AGGTTTATGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028613.1
CDS	128..1552
Translation	MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGRFSLSQWDPMITTLPGLYLVSVGVVKPASWLLGWSEHVICSIGVLRFVNLLFSVGNFYLLYLLFRKVQPRNKASSSIQRILSTLTLAVFPTLYFFNFLYYTEAGSVFFTLFAYLMCLYGNHRTSALLGFCGFMFRQTNIIWAAFCAGHLIAQKCSEAWKIELQKKKEERLAPTKGPLSELRRVLQFLLVYAMSLKNLRMLFLLTWPYVLLLLAFFAFVVVNGGIVVGDRSSHEACLHFPQLFYFFSFTAFFSFPHLLSLTKVKTFLSLVWKRRVQFSVVTLVSILLVWKFTYVHKYLLADNRHYTFYVWKRVFQRHEVVKYLLVPAYIFAGWAIADSLKAKSIFWNLMFFVCLVASTVPQKLLEFRYFILPYIIYRLNIPLPPISRLVCELGCYTVVNFVTFYIFLNKTFQWPNSQDIQRFMW

Target ORF information:

RefSeq Version	NM_001033441.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus asparagine-linked glycosylation 10B (alpha-1,2-glucosyltransferase) (Alg10b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033441.3

ORF Insert Sequence:

ATGGCGCAGC TGGAGGGCTA TTACTTCTCG GCCGCCTTGA GCTGCACCTT CCTGGTGTCC 
TGCCTGCTCT TCTCCGCCTT CAGCCGCGCT CTGCGGGAGC CGTACATGGA CGAGATCTTC 
CACCTGCCGC AGGCGCAGCG GTACTGCGAG GGCCGCTTCT CCCTGTCGCA GTGGGATCCT 
ATGATCACTA CATTACCTGG CCTGTATCTG GTGTCGGTCG GTGTGGTCAA ACCTGCCAGC 
TGGCTCCTTG GATGGTCTGA GCATGTGATC TGCTCCATTG GGGTGCTCAG ATTTGTCAAT 
CTTCTCTTCA GCGTTGGCAA CTTCTACTTG CTGTATTTGC TCTTCAGGAA GGTGCAACCC 
AGAAACAAGG CTTCTTCAAG TATCCAGAGA ATCTTGTCAA CATTAACCCT AGCAGTGTTT 
CCAACCCTCT ATTTTTTTAA CTTCCTTTAC TATACAGAAG CTGGGTCTGT ATTCTTCACT 
CTTTTTGCTT ATTTGATGTG TCTTTATGGC AACCATAGGA CCTCGGCCTT GCTTGGATTT 
TGTGGTTTCA TGTTTCGTCA GACCAACATC ATCTGGGCGG CCTTCTGTGC AGGACACCTC 
ATTGCACAGA AGTGCAGTGA AGCCTGGAAG ATTGAGCTGC AGAAGAAGAA GGAGGAGAGG 
CTTGCGCCCA CGAAGGGACC GCTCTCGGAG CTCAGAAGAG TGCTGCAGTT TCTGCTGGTG 
TACGCCATGT CCCTAAAGAA CCTGAGAATG CTCTTCCTGT TGACCTGGCC CTACGTGCTT 
CTGCTGTTGG CGTTCTTTGC CTTTGTGGTA GTTAATGGTG GCATTGTTGT TGGTGACCGG 
AGTAGTCACG AGGCCTGTCT CCATTTTCCT CAGTTGTTCT ATTTCTTCTC CTTTACTGCC 
TTTTTCTCCT TCCCTCACCT ACTTTCTCTG ACCAAAGTCA AGACTTTCCT TAGCTTAGTT 
TGGAAGCGTA GAGTCCAGTT CTCTGTGGTT ACGTTAGTCT CGATACTTTT GGTTTGGAAA 
TTCACTTATG TCCATAAGTA TTTACTGGCA GACAATAGGC ATTACACATT TTATGTGTGG 
AAAAGAGTAT TTCAGAGACA TGAAGTTGTC AAATATTTAT TAGTTCCAGC CTACATTTTT 
GCTGGTTGGG CTATAGCTGA CTCTTTAAAG GCTAAGTCAA TTTTCTGGAA TTTAATGTTT 
TTTGTATGCT TGGTTGCTTC TACAGTTCCT CAGAAACTAC TAGAATTCCG TTACTTCATT 
CTACCATACA TTATTTATAG GCTTAACATA CCTCTGCCAC CCATATCTAG ACTCGTTTGT 
GAACTGGGTT GCTATACAGT TGTTAATTTT GTAACTTTTT ATATCTTTCT GAACAAGACT 
TTTCAGTGGC CAAATAGTCA GGACATCCAA AGGTTTATGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b) causes nonsyndromic hearing impairment in mice (Mus musculus).
PloS one8(11)e80408(2013)
Probst FJ,Corrigan RR,Del Gaudio D,Salinger AP,Lorenzo I,Gao SS,Chiu I,Xia A,Oghalai JS,Justice MJ

Functional genetic analysis of mouse chromosome 11.
Nature425(6953)81-6(2003 Sep)
Kile BT,Hentges KE,Clark AT,Nakamura H,Salinger AP,Liu B,Box N,Stockton DW,Johnson RL,Behringer RR,Bradley A,Justice MJ

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Alg10b cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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