Gchfr cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gchfr
Entrez Gene ID	320415
Full Name	GTP cyclohydrolase I feedback regulator
Synonyms	2010323F13Rik,GFRP,P35
General protein information	Preferred Names GTP cyclohydrolase I feedback regulator Names GTP cyclohydrolase 1 feedback regulatory protein GTP cyclohydrolase I feedback regulatory protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_177157.4	NP_796131.1	GTP cyclohydrolase 1 feedback regulatory protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GCHFR	NP_005249.1
Canis lupus familiaris (dog)	GCHFR	XP_544622.2
Mus musculus (house mouse)	Gchfr	NP_796131.1
Gallus gallus (chicken)	GCHFR	NP_001186451.1
Pan troglodytes (chimpanzee)	GCHFR	XP_001146591.1
Xenopus tropicalis (tropical clawed frog)	gchfr	NP_998880.1
	GCHFR	XP_001097382.2
Bos taurus (cattle)	GCHFR	NP_001107998.1
Rattus norvegicus (Norway rat)	Gchfr	NP_598279.1
Danio rerio (zebrafish)	gchfr	NP_957056.1
Caenorhabditis elegans (roundworm)	gfrp-1	NP_001041021.2

Gene Ontology
Bibliography

Related articles in PubMed

Pet-1 Controls Tetrahydrobiopterin Pathway and Slc22a3 Transporter Genes in Serotonin Neurons.
Wyler SC, Donovan LJ, Yeager M, Deneris E
ACS chemical neuroscience6(7)1198-205(2015 Jul)

GTP cyclohydrolase I phosphorylation and interaction with GTP cyclohydrolase feedback regulatory protein provide novel regulation of endothelial tetrahydrobiopterin and nitric oxide.
Li L, Rezvan A, Salerno JC, Husain A, Kwon K, Jo H, Harrison DG, Chen W
Circulation research106(2)328-36(2010 Feb)

GTP cyclohydrolase I expression, protein, and activity determine intracellular tetrahydrobiopterin levels, independent of GTP cyclohydrolase feedback regulatory protein expression.
Tatham AL, Crabtree MJ, Warrick N, Cai S, Alp NJ, Channon KM
The Journal of biological chemistry284(20)13660-8(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies provide a new mechanism for regulation of endothelial GTPCH-1 by its phosphorylation and interplay with GFRP.
Title: GTP cyclohydrolase I phosphorylation and interaction with GTP cyclohydrolase feedback regulatory protein provide novel regulation of endothelial tetrahydrobiopterin and nitric oxide.

GTPCH expression is the primary regulator of BH4 levels, and changes in GTPCH or BH4 are not necessarily accompanied by changes in GFRP expression.
Title: GTP cyclohydrolase I expression, protein, and activity determine intracellular tetrahydrobiopterin levels, independent of GTP cyclohydrolase feedback regulatory protein expression.

The following Gchfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gchfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05997	NM_177157.4 Latest version!	Mus musculus GTP cyclohydrolase I feedback regulator (Gchfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gchfr ( NM_177157.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796131.1 Mus musculus GTP cyclohydrolase I feedback regulator (Gchfr), mRNA.

Price & Availability↑

CloneID	OMu05997
Clone ID Related Accession (Same CDS sequence)	NM_177157.4
Accession Version	NM_177157.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	GTP cyclohydrolase 1 feedback regulatory protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK028116.1. On Apr 5, 2007 this sequence version replaced NM_177157.3. ##Evidence-Data-START## Transcript exon combination :: AK028116.1, AK028122.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTATC TGCTCATCAG CACTCAGATC CGTATGGAGG TGGGCCCCAC CATGGTGGGT 
GATGAACACT CGGATCCGGA GCTGATGCAG CACCTGGGGG CCTCCAAGAG AAGTGTCTTG 
GGAAACAACT TTTACGAATA CTACGTCAAC GACCCTCCTC GCATAGTCCT GGACAAGCTG 
GAATGCAAGG GCTTCCGAGT GCTGAGCATG ACAGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796131.1
CDS	51..305
Translation	MPYLLISTQIRMEVGPTMVGDEHSDPELMQHLGASKRSVLGNNFYEYYVNDPPRIVLDKLECKGFRVLSMTGVGQTLVWCLHKE

Target ORF information:

RefSeq Version	NM_177157.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTP cyclohydrolase I feedback regulator (Gchfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177157.4

ORF Insert Sequence:

ATGCCCTATC TGCTCATCAG CACTCAGATC CGTATGGAGG TGGGCCCCAC CATGGTGGGT 
GATGAACACT CGGATCCGGA GCTGATGCAG CACCTGGGGG CCTCCAAGAG AAGTGTCTTG 
GGAAACAACT TTTACGAATA CTACGTCAAC GACCCTCCTC GCATAGTCCT GGACAAGCTG 
GAATGCAAGG GCTTCCGAGT GCTGAGCATG ACAGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pet-1 Controls Tetrahydrobiopterin Pathway and Slc22a3 Transporter Genes in Serotonin Neurons.
ACS chemical neuroscience6(7)1198-205(2015 Jul)
Wyler SC,Donovan LJ,Yeager M,Deneris E

GTP cyclohydrolase I phosphorylation and interaction with GTP cyclohydrolase feedback regulatory protein provide novel regulation of endothelial tetrahydrobiopterin and nitric oxide.
Circulation research106(2)328-36(2010 Feb)
Li L,Rezvan A,Salerno JC,Husain A,Kwon K,Jo H,Harrison DG,Chen W

GTP cyclohydrolase I expression, protein, and activity determine intracellular tetrahydrobiopterin levels, independent of GTP cyclohydrolase feedback regulatory protein expression.
The Journal of biological chemistry284(20)13660-8(2009 May)
Tatham AL,Crabtree MJ,Warrick N,Cai S,Alp NJ,Channon KM