Lcmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lcmt1
Entrez Gene ID	30949
Full Name	leucine carboxyl methyltransferase 1
Synonyms	AL033290,LCMT-1,Lcmt
General protein information	Preferred Names leucine carboxyl methyltransferase 1 Names leucine carboxyl methyltransferase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030242666.1	XP_030098526.1	leucine carboxyl methyltransferase 1 isoform X1
XM_006507917.3	XP_006507980.1	leucine carboxyl methyltransferase 1 isoform X1
NM_001355050.1	NP_001341979.1	leucine carboxyl methyltransferase 1 isoform 2
NM_025304.3	NP_079580.2	leucine carboxyl methyltransferase 1 isoform 1

Pathways from BioSystems

Homologies

	LCMT1	XP_001099127.1
Drosophila melanogaster (fruit fly)	CG3793	NP_609766.1
Caenorhabditis elegans (roundworm)	CELE_B0285.4	NP_497875.1
Oryza sativa (rice)	Os06g0140100	NP_001056749.1
Xenopus tropicalis (tropical clawed frog)	lcmt1	NP_001016033.1
Pan troglodytes (chimpanzee)	LCMT1	XP_001164891.1
Canis lupus familiaris (dog)	LCMT1	XP_005621453.1
Bos taurus (cattle)	LCMT1	NP_001069956.1
Mus musculus (house mouse)	Lcmt1	NP_079580.2
Gallus gallus (chicken)	LCMT1	NP_001006167.2
Arabidopsis thaliana (thale cress)	AT1G02100	NP_973741.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008768	XP_314892.4
Homo sapiens (human)	LCMT1	NP_057393.2
Rattus norvegicus (Norway rat)	Lcmt1	NP_955437.1
Danio rerio (zebrafish)	lcmt1	NP_001004645.1

Gene Ontology
Bibliography

Related articles in PubMed

Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
Hwang J, Lee JA, Pallas DC
The Journal of biological chemistry291(40)21008-21019(2016 Sep)

Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.
MacKay KB, Tu Y, Young SG, Clarke SG
PloS one8(6)e65967(2013)

Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.
Sontag JM, Nunbhakdi-Craig V, Mitterhuber M, Ogris E, Sontag E
Journal of neurochemistry115(6)1455-65(2010 Dec)

Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells.
Lee JA, Pallas DC
The Journal of biological chemistry282(42)30974-84(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LCMT-1 homozygous knock-out MEFs exhibited hyperphosphorylation of HDAC3, a reported target of the methylation-dependent PP4R1-PP4c complex. Collectively, our data suggest that LCMT-1 coordinately regulates the carboxyl methylation of PP2A-related phosphatases and, consequently, their holoenzyme assembly and function.
Title: Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.

In LCMT1 deficiency enzyme activity and methylation of PP2A are reduced in a coordinate fashion, suggesting that LCMT1 is the only PP2A methyltransferase.
Title: Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.

Enhanced expression of LCMT1 in cultured neuroblastoma cells, which increases endogenous methylated catalytic C subunit and Balpha levels, induces changes in F-actin organization.
Title: Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.

LCMT-1 is important for normal progression through mitosis and cell survival and is essential for embryonic development
Title: Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells.

The following Lcmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lcmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00244	NM_025304.3 Latest version!	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu39703	NM_001355050.1 Latest version!	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu39703	XM_006507917.3 Latest version!	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu39703	XM_030242666.1 Latest version!	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lcmt1 ( NM_025304.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079580.2 Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00244
Clone ID Related Accession (Same CDS sequence)	NM_025304.3
Accession Version	NM_025304.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Mus musculus(house mouse)
Product	leucine carboxyl methyltransferase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU571243.1, AK003424.1 and BB686087.1. On or before Mar 23, 2004 this sequence version replaced XM_355938.1, NM_025304.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.249183.1, AK003424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCCA GCTCGAGGGA GTCCTCGTTC TCTTCCTGCT CGTCGAGCTG TGACTTGGAC 
GACGAGGGAG TGCGCGGCAC CTGCGAAGAT GCTTCTCTCT GCAAGAGGTT TGCGGTGAGC 
ATTGGCTACT GGCATGATCC GTACATCGAG CATCTTGTAA GGCAGTCTAA AGAGAGGAAG 
GCCCCCGAAA TTAACAGAGG ATATTTTGCC CGAGTTCATG GTGTCAGTCA GCTTATTAAA 
GCTTTTCTCC GAAAGACAGA ATGCCATTGC CAAATTCTCA ACCTTGGGGC TGGGATGGAT 
ACCACCTTCT GGAAATTAAA GGATGAAGGT CTTCTCCCAA ATAAATATTT TGAAGTCGAC 
TTTCCAATGA TAGTCACAAG AAAGCTGCTC ACCATCAAAA GCAAGCCGCT TTTATTCAGA 
CCCATCATGG AGTTACACCC AGAGGACACG CTTCAAATGG ATAGTCACAT GCTGGATTCA 
AAAAGATATG CCATCATTGG AGCAGATCTC CGAGACCTTT CTGAGCTGGA AGAGAAACTA 
AAGAAATGTA ACATGAATAC ACAATTGCCA ACACTCCTGA TAACTGAATG TGTGCTGGTT 
TACATGACTC CAGAGCAGTC AGCCAACCTC CTGAAATGGG CAGCCAGCAG TTTTGAGACA 
GCCATGTTCA TAAACTATGA GCAGGTCAAC ATGGACGACC GGTTTGGGCA GATCATGATT 
GAAAACCTCC GGAGGCGAAG TTGTGACCTG GCAGGAGTGG AAACCTGCAA ATCACTGGAG 
TCCCAGAAGG AACGGCTGCT GTTGAATGGG TGGGAGACAG CGTCTGCAGT GAACATGATG 
GAGCTGTACA GTGGGCTACC TCGGGCAGAG GTAAACAGAA TAGAATCCCT CGAGTTCCTG 
GATGAGATGG AGCTCCTGGA GCAGCTCATG CGCCATTACT GCCTGTGCTG GGCCACCCGC 
GGCGGCCAGG AGCTTGGTTT GAAGGAGATA ACCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079580.2
CDS	168..1166
Translation	MASSSRESSFSSCSSSCDLDDEGVRGTCEDASLCKRFAVSIGYWHDPYIEHLVRQSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQILNLGAGMDTTFWKLKDEGLLPNKYFEVDFPMIVTRKLLTIKSKPLLFRPIMELHPEDTLQMDSHMLDSKRYAIIGADLRDLSELEEKLKKCNMNTQLPTLLITECVLVYMTPEQSANLLKWAASSFETAMFINYEQVNMDDRFGQIMIENLRRRSCDLAGVETCKSLESQKERLLLNGWETASAVNMMELYSGLPRAEVNRIESLEFLDEMELLEQLMRHYCLCWATRGGQELGLKEITY

Target ORF information:

RefSeq Version	NM_025304.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025304.3

ORF Insert Sequence:

ATGGCCTCCA GCTCGAGGGA GTCCTCGTTC TCTTCCTGCT CGTCGAGCTG TGACTTGGAC 
GACGAGGGAG TGCGCGGCAC CTGCGAAGAT GCTTCTCTCT GCAAGAGGTT TGCGGTGAGC 
ATTGGCTACT GGCATGATCC GTACATCGAG CATCTTGTAA GGCAGTCTAA AGAGAGGAAG 
GCCCCCGAAA TTAACAGAGG ATATTTTGCC CGAGTTCATG GTGTCAGTCA GCTTATTAAA 
GCTTTTCTCC GAAAGACAGA ATGCCATTGC CAAATTCTCA ACCTTGGGGC TGGGATGGAT 
ACCACCTTCT GGAAATTAAA GGATGAAGGT CTTCTCCCAA ATAAATATTT TGAAGTCGAC 
TTTCCAATGA TAGTCACAAG AAAGCTGCTC ACCATCAAAA GCAAGCCGCT TTTATTCAGA 
CCCATCATGG AGTTACACCC AGAGGACACG CTTCAAATGG ATAGTCACAT GCTGGATTCA 
AAAAGATATG CCATCATTGG AGCAGATCTC CGAGACCTTT CTGAGCTGGA AGAGAAACTA 
AAGAAATGTA ACATGAATAC ACAATTGCCA ACACTCCTGA TAACTGAATG TGTGCTGGTT 
TACATGACTC CAGAGCAGTC AGCCAACCTC CTGAAATGGG CAGCCAGCAG TTTTGAGACA 
GCCATGTTCA TAAACTATGA GCAGGTCAAC ATGGACGACC GGTTTGGGCA GATCATGATT 
GAAAACCTCC GGAGGCGAAG TTGTGACCTG GCAGGAGTGG AAACCTGCAA ATCACTGGAG 
TCCCAGAAGG AACGGCTGCT GTTGAATGGG TGGGAGACAG CGTCTGCAGT GAACATGATG 
GAGCTGTACA GTGGGCTACC TCGGGCAGAG GTAAACAGAA TAGAATCCCT CGAGTTCCTG 
GATGAGATGG AGCTCCTGGA GCAGCTCATG CGCCATTACT GCCTGTGCTG GGCCACCCGC 
GGCGGCCAGG AGCTTGGTTT GAAGGAGATA ACCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lcmt1 ( NM_001355050.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001341979.1 Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu39703
Clone ID Related Accession (Same CDS sequence)	XM_030242666.1 , XM_006507917.3 , NM_001355050.1
Accession Version	NM_001355050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Mus musculus(house mouse)
Product	leucine carboxyl methyltransferase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121956.3. On Sep 6, 2017 this sequence version replaced XM_006507917.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BG870400.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG 
GAAGAGAAAC TAAAGAAATG TAACATGAAT ACACAATTGC CAACACTCCT GATAACTGAA 
TGTGTGCTGG TTTACATGAC TCCAGAGCAG TCAGCCAACC TCCTGAAATG GGCAGCCAGC 
AGTTTTGAGA CAGCCATGTT CATAAACTAT GAGCAGGTCA ACATGGACGA CCGGTTTGGG 
CAGATCATGA TTGAAAACCT CCGGAGGCGA AGTTGTGACC TGGCAGGAGT GGAAACCTGC 
AAATCACTGG AGTCCCAGAA GGAACGGCTG CTGTTGAATG GGTGGGAGAC AGCGTCTGCA 
GTGAACATGA TGGAGCTGTA CAGTGGGCTA CCTCGGGCAG AGGTAAACAG AATAGAATCC 
CTCGAGTTCC TGGATGAGAT GGAGCTCCTG GAGCAGCTCA TGCGCCATTA CTGCCTGTGC 
TGGGCCACCC GCGGCGGCCA GGAGCTTGGT TTGAAGGAGA TAACCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341979.1
CDS	628..1158
Translation	MLDSKRYAIIGADLRDLSELEEKLKKCNMNTQLPTLLITECVLVYMTPEQSANLLKWAASSFETAMFINYEQVNMDDRFGQIMIENLRRRSCDLAGVETCKSLESQKERLLLNGWETASAVNMMELYSGLPRAEVNRIESLEFLDEMELLEQLMRHYCLCWATRGGQELGLKEITY

Target ORF information:

RefSeq Version	NM_001355050.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355050.1

ORF Insert Sequence:

ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG 
GAAGAGAAAC TAAAGAAATG TAACATGAAT ACACAATTGC CAACACTCCT GATAACTGAA 
TGTGTGCTGG TTTACATGAC TCCAGAGCAG TCAGCCAACC TCCTGAAATG GGCAGCCAGC 
AGTTTTGAGA CAGCCATGTT CATAAACTAT GAGCAGGTCA ACATGGACGA CCGGTTTGGG 
CAGATCATGA TTGAAAACCT CCGGAGGCGA AGTTGTGACC TGGCAGGAGT GGAAACCTGC 
AAATCACTGG AGTCCCAGAA GGAACGGCTG CTGTTGAATG GGTGGGAGAC AGCGTCTGCA 
GTGAACATGA TGGAGCTGTA CAGTGGGCTA CCTCGGGCAG AGGTAAACAG AATAGAATCC 
CTCGAGTTCC TGGATGAGAT GGAGCTCCTG GAGCAGCTCA TGCGCCATTA CTGCCTGTGC 
TGGGCCACCC GCGGCGGCCA GGAGCTTGGT TTGAAGGAGA TAACCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lcmt1 ( XM_006507917.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507980.1 Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39703
Clone ID Related Accession (Same CDS sequence)	XM_030242666.1 , XM_006507917.3 , NM_001355050.1
Accession Version	XM_006507917.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	leucine carboxyl methyltransferase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG 
GAAGAGAAAC TAAAGAAATG TAACATGAAT ACACAATTGC CAACACTCCT GATAACTGAA 
TGTGTGCTGG TTTACATGAC TCCAGAGCAG TCAGCCAACC TCCTGAAATG GGCAGCCAGC 
AGTTTTGAGA CAGCCATGTT CATAAACTAT GAGCAGGTCA ACATGGACGA CCGGTTTGGG 
CAGATCATGA TTGAAAACCT CCGGAGGCGA AGTTGTGACC TGGCAGGAGT GGAAACCTGC 
AAATCACTGG AGTCCCAGAA GGAACGGCTG CTGTTGAATG GGTGGGAGAC AGCGTCTGCA 
GTGAACATGA TGGAGCTGTA CAGTGGGCTA CCTCGGGCAG AGGTAAACAG AATAGAATCC 
CTCGAGTTCC TGGATGAGAT GGAGCTCCTG GAGCAGCTCA TGCGCCATTA CTGCCTGTGC 
TGGGCCACCC GCGGCGGCCA GGAGCTTGGT TTGAAGGAGA TAACCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507980.1
CDS	628..1158
Translation	MLDSKRYAIIGADLRDLSELEEKLKKCNMNTQLPTLLITECVLVYMTPEQSANLLKWAASSFETAMFINYEQVNMDDRFGQIMIENLRRRSCDLAGVETCKSLESQKERLLLNGWETASAVNMMELYSGLPRAEVNRIESLEFLDEMELLEQLMRHYCLCWATRGGQELGLKEITY

Target ORF information:

RefSeq Version	XM_006507917.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507917.3

ORF Insert Sequence:

ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG 
GAAGAGAAAC TAAAGAAATG TAACATGAAT ACACAATTGC CAACACTCCT GATAACTGAA 
TGTGTGCTGG TTTACATGAC TCCAGAGCAG TCAGCCAACC TCCTGAAATG GGCAGCCAGC 
AGTTTTGAGA CAGCCATGTT CATAAACTAT GAGCAGGTCA ACATGGACGA CCGGTTTGGG 
CAGATCATGA TTGAAAACCT CCGGAGGCGA AGTTGTGACC TGGCAGGAGT GGAAACCTGC 
AAATCACTGG AGTCCCAGAA GGAACGGCTG CTGTTGAATG GGTGGGAGAC AGCGTCTGCA 
GTGAACATGA TGGAGCTGTA CAGTGGGCTA CCTCGGGCAG AGGTAAACAG AATAGAATCC 
CTCGAGTTCC TGGATGAGAT GGAGCTCCTG GAGCAGCTCA TGCGCCATTA CTGCCTGTGC 
TGGGCCACCC GCGGCGGCCA GGAGCTTGGT TTGAAGGAGA TAACCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lcmt1 ( XM_030242666.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098526.1 Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39703
Clone ID Related Accession (Same CDS sequence)	XM_030242666.1 , XM_006507917.3 , NM_001355050.1
Accession Version	XM_030242666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leucine carboxyl methyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG 
GAAGAGAAAC TAAAGAAATG TAACATGAAT ACACAATTGC CAACACTCCT GATAACTGAA 
TGTGTGCTGG TTTACATGAC TCCAGAGCAG TCAGCCAACC TCCTGAAATG GGCAGCCAGC 
AGTTTTGAGA CAGCCATGTT CATAAACTAT GAGCAGGTCA ACATGGACGA CCGGTTTGGG 
CAGATCATGA TTGAAAACCT CCGGAGGCGA AGTTGTGACC TGGCAGGAGT GGAAACCTGC 
AAATCACTGG AGTCCCAGAA GGAACGGCTG CTGTTGAATG GGTGGGAGAC AGCGTCTGCA 
GTGAACATGA TGGAGCTGTA CAGTGGGCTA CCTCGGGCAG AGGTAAACAG AATAGAATCC 
CTCGAGTTCC TGGATGAGAT GGAGCTCCTG GAGCAGCTCA TGCGCCATTA CTGCCTGTGC 
TGGGCCACCC GCGGCGGCCA GGAGCTTGGT TTGAAGGAGA TAACCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098526.1
CDS	1434..1964
Translation	MLDSKRYAIIGADLRDLSELEEKLKKCNMNTQLPTLLITECVLVYMTPEQSANLLKWAASSFETAMFINYEQVNMDDRFGQIMIENLRRRSCDLAGVETCKSLESQKERLLLNGWETASAVNMMELYSGLPRAEVNRIESLEFLDEMELLEQLMRHYCLCWATRGGQELGLKEITY

Target ORF information:

RefSeq Version	XM_030242666.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242666.1

ORF Insert Sequence:

ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG 
GAAGAGAAAC TAAAGAAATG TAACATGAAT ACACAATTGC CAACACTCCT GATAACTGAA 
TGTGTGCTGG TTTACATGAC TCCAGAGCAG TCAGCCAACC TCCTGAAATG GGCAGCCAGC 
AGTTTTGAGA CAGCCATGTT CATAAACTAT GAGCAGGTCA ACATGGACGA CCGGTTTGGG 
CAGATCATGA TTGAAAACCT CCGGAGGCGA AGTTGTGACC TGGCAGGAGT GGAAACCTGC 
AAATCACTGG AGTCCCAGAA GGAACGGCTG CTGTTGAATG GGTGGGAGAC AGCGTCTGCA 
GTGAACATGA TGGAGCTGTA CAGTGGGCTA CCTCGGGCAG AGGTAAACAG AATAGAATCC 
CTCGAGTTCC TGGATGAGAT GGAGCTCCTG GAGCAGCTCA TGCGCCATTA CTGCCTGTGC 
TGGGCCACCC GCGGCGGCCA GGAGCTTGGT TTGAAGGAGA TAACCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
The Journal of biological chemistry291(40)21008-21019(2016 Sep)
Hwang J,Lee JA,Pallas DC

Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.
PloS one8(6)e65967(2013)
MacKay KB,Tu Y,Young SG,Clarke SG

Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.
Journal of neurochemistry115(6)1455-65(2010 Dec)
Sontag JM,Nunbhakdi-Craig V,Mitterhuber M,Ogris E,Sontag E

Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells.
The Journal of biological chemistry282(42)30974-84(2007 Oct)
Lee JA,Pallas DC