Efhd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Efhd2
Entrez Gene ID	27984
Full Name	EF hand domain containing 2
Synonyms	2600015J22Rik,AA408606,D4Wsu27e
General protein information	Preferred Names EF hand domain containing 2 Names EF-hand domain-containing protein D2 swiprosin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E1\|4 74.75 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_025994.3	NP_080270.2	EF-hand domain-containing protein D2

Pathways from BioSystems

Homologies

	EFHD2	XP_001082735.1
Rattus norvegicus (Norway rat)	Efhd2	NP_001026818.1
Xenopus tropicalis (tropical clawed frog)	efhd2	NP_001011261.1
Bos taurus (cattle)	EFHD2	NP_001096715.1
Mus musculus (house mouse)	Efhd2	NP_080270.2
Caenorhabditis elegans (roundworm)	CELE_Y48B6A.6	NP_871966.2
Homo sapiens (human)	EFHD2	NP_077305.2
Pan troglodytes (chimpanzee)	EFHD2	XP_001156210.3
Gallus gallus (chicken)	EFHD2	XP_004947548.1
Danio rerio (zebrafish)	efhd2	NP_001038676.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009528	XP_310162.3
Drosophila melanogaster (fruit fly)	CG10641	NP_609925.1

Gene Ontology
Bibliography

Related articles in PubMed

Impact of Swiprosin-1/Efhd2 on Adult Hippocampal Neurogenesis.
Regensburger M, Prots I, Reimer D, Brachs S, Loskarn S, Lie DC, Mielenz D, Winner B
Stem cell reports(2018 Jan)

EFhd2/swiprosin-1 regulates LPS-induced macrophage recruitment via enhancing actin polymerization and cell migration.
Tu Y, Zhang L, Tong L, Wang Y, Zhang S, Wang R, Li L, Wang Z
International immunopharmacology55263-271(2017 Dec)

Low level of swiprosin-1/EFhd2 in vestibular nuclei of spontaneously hypersensitive motion sickness mice.
Wang ZB, Han P, Tong LC, Luo Y, Su WH, Wei X, Yu XH, Liu WY, Zhang XH, Lei H, Li ZZ, Wang F, Chen JG, Ma TH, Su DF, Li L
Scientific reports740986(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a role is found for EFhd2 as a negative regulator of germinal center-dependent humoral type 2 immunity, with implications for the generation of IgE.
Title: Swiprosin-1/EFhd2 limits germinal center responses and humoral type 2 immunity.

EFhd2 is a neuronal protein that interferes with kinesin-mediated transport.
Title: The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons.

These results show that EFhd2 is not essential for platelet function in mice indicating that other cytoskeletal adaptors may functionally compensate its loss.
Title: The adaptor protein Swiprosin-1/EFhd2 is dispensable for platelet function in mice.

Swiprosin-1 is involved in regulating the accessibility of F-actin to cofilin through the clustering of F-actin and the activity of Swiprosin-1 is highly dependent on its phosphorylation status at Ser183
Title: Swiprosin-1 modulates actin dynamics by regulating the F-actin accessibility to cofilin.

EFhd2 may play an important role in the pathobiology of tau-mediated neurodegeneration.
Title: EFhd2 is a novel amyloid protein associated with pathological tau in Alzheimer's disease.

The following Efhd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Efhd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22417	NM_025994.3 Latest version!	Mus musculus EF hand domain containing 2 (Efhd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Efhd2 ( NM_025994.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080270.2 Mus musculus EF hand domain containing 2 (Efhd2), mRNA.

Price & Availability↑

CloneID	OMu22417
Clone ID Related Accession (Same CDS sequence)	NM_025994.3
Accession Version	NM_025994.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	EF-hand domain-containing protein D2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK048475.1. On Apr 5, 2007 this sequence version replaced NM_025994.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK048475.1, AK159367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGG ACGAGTTGGC CAGCAAGCTG AGCCGGAGGC TGCAGATGGA GGGCGAAGGC 
GGCGAGGCGA CGGAGCAGCC GGGGCTCAAC GGGGCGGCGG CGGCGGCGGC GGCCGAGGCT 
CCCGACGAGA CTGCCCAGGC GTTGGGCAGC GCGGACGACG AGCTGAGCGC CAAGCTGCTG 
CGGCGCGCGG ACCTCAACCA GGGCATCGGC GAGCCACAGT CGCCCAGCCG CCGCGTCTTC 
AACCCCTACA CCGAGTTCAA GGAGTTCTCC AGGAAGCAGA TCAAAGACAT GGAGAAGATG 
TTCAAGCAGT ATGATGCCGG CAGGGATGGC TTCATCGACC TGATGGAGCT GAAACTCATG 
ATGGAGAAGC TGGGGGCCCC CCAGACACAC TTGGGCCTCA AGAGTATGAT CCAGGAGGTG 
GACGAGGATT TCGACAGCAA ACTCAGCTTC CGGGAGTTCC TTCTGATCTT CCGCAAGGCA 
GCAGCAGGGG AGTTGCAGGA AGACAGCGGC TTGCACGTCC TGGCCCGCCT GTCCGAGATC 
GATGTCTCCA CAGAGGGCGT TAAGGGTGCC AAGAACTTCT TCGAGGCCAA GGTACAGGCC 
ATCAACGTGT CCAGCCGCTT TGAGGAAGAG ATCAAAGCTG AGCAAGAGGA AAGGAAGAAG 
CAGGCTGAGG AGGTGAAGCA GCGGAAAGCG GCCTTTAAGG AGCTGCAGTC CACGTTCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080270.2
CDS	54..776
Translation	MATDELASKLSRRLQMEGEGGEATEQPGLNGAAAAAAAEAPDETAQALGSADDELSAKLLRRADLNQGIGEPQSPSRRVFNPYTEFKEFSRKQIKDMEKMFKQYDAGRDGFIDLMELKLMMEKLGAPQTHLGLKSMIQEVDEDFDSKLSFREFLLIFRKAAAGELQEDSGLHVLARLSEIDVSTEGVKGAKNFFEAKVQAINVSSRFEEEIKAEQEERKKQAEEVKQRKAAFKELQSTFK

Target ORF information:

RefSeq Version	NM_025994.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EF hand domain containing 2 (Efhd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025994.3

ORF Insert Sequence:

ATGGCCACGG ACGAGTTGGC CAGCAAGCTG AGCCGGAGGC TGCAGATGGA GGGCGAAGGC 
GGCGAGGCGA CGGAGCAGCC GGGGCTCAAC GGGGCGGCGG CGGCGGCGGC GGCCGAGGCT 
CCCGACGAGA CTGCCCAGGC GTTGGGCAGC GCGGACGACG AGCTGAGCGC CAAGCTGCTG 
CGGCGCGCGG ACCTCAACCA GGGCATCGGC GAGCCACAGT CGCCCAGCCG CCGCGTCTTC 
AACCCCTACA CCGAGTTCAA GGAGTTCTCC AGGAAGCAGA TCAAAGACAT GGAGAAGATG 
TTCAAGCAGT ATGATGCCGG CAGGGATGGC TTCATCGACC TGATGGAGCT GAAACTCATG 
ATGGAGAAGC TGGGGGCCCC CCAGACACAC TTGGGCCTCA AGAGTATGAT CCAGGAGGTG 
GACGAGGATT TCGACAGCAA ACTCAGCTTC CGGGAGTTCC TTCTGATCTT CCGCAAGGCA 
GCAGCAGGGG AGTTGCAGGA AGACAGCGGC TTGCACGTCC TGGCCCGCCT GTCCGAGATC 
GATGTCTCCA CAGAGGGCGT TAAGGGTGCC AAGAACTTCT TCGAGGCCAA GGTACAGGCC 
ATCAACGTGT CCAGCCGCTT TGAGGAAGAG ATCAAAGCTG AGCAAGAGGA AAGGAAGAAG 
CAGGCTGAGG AGGTGAAGCA GCGGAAAGCG GCCTTTAAGG AGCTGCAGTC CACGTTCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impact of Swiprosin-1/Efhd2 on Adult Hippocampal Neurogenesis.
Stem cell reports(2018 Jan)
Regensburger M,Prots I,Reimer D,Brachs S,Loskarn S,Lie DC,Mielenz D,Winner B

EFhd2/swiprosin-1 regulates LPS-induced macrophage recruitment via enhancing actin polymerization and cell migration.
International immunopharmacology55263-271(2017 Dec)
Tu Y,Zhang L,Tong L,Wang Y,Zhang S,Wang R,Li L,Wang Z

Low level of swiprosin-1/EFhd2 in vestibular nuclei of spontaneously hypersensitive motion sickness mice.
Scientific reports740986(2017 Jan)
Wang ZB,Han P,Tong LC,Luo Y,Su WH,Wei X,Yu XH,Liu WY,Zhang XH,Lei H,Li ZZ,Wang F,Chen JG,Ma TH,Su DF,Li L