ORF » Species Summary » Mus musculus » Nit1 cDNA ORF clone

Nit1 cDNA ORF clone, Mus musculus(house mouse)

The following Nit1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nit1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu03250 NM_001242580.1
Latest version!		 Mus musculus nitrilase 1 (Nit1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OMu09387 NM_012049.2
Latest version!		 Mus musculus nitrilase 1 (Nit1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $188.30
$269.00
OMu03250 XM_006496866.4
Latest version!		 Mus musculus nitrilase 1 (Nit1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OMu03250 XM_006496866.3 Mus musculus nitrilase 1 (Nit1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Nit1 ( NM_001242580.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001229509.1 Mus musculus nitrilase 1 (Nit1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OMu03250
    Clone ID Related Accession (Same CDS sequence) XM_006496866.3 , NM_001242580.1 , XM_006496866.4
    Accession Version NM_001242580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Mus musculus(house mouse)
    Product deaminated glutathione amidase isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087229.27 and AC084821.26. On Jun 15, 2011 this sequence version replaced NR_033728.1. Transcript Variant: This variant (2) uses an alternate splice site at the 5' end compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (2) with a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK002269.1, AK004988.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCCTCAT CAACTTCCTG GGAGCTGCCC CTGGTGGCTG TGTGCCAGGT AACATCAACA 
    CCAAACAAGC AAGAGAACTT TAAAACATGT GCTGAGTTGG TTCAAGAGGC TGCCAGACTG 
    GGTGCTTGCC TGGCCTTTCT GCCTGAGGCA TTTGACTTTA TTGCACGAAA CCCTGCCGAG 
    ACATTACTCC TGTCCGAACC ACTGAATGGG GATCTTTTGG GCCAATATAG CCAGCTTGCC 
    AGGGAATGTG GAATCTGGCT GTCCTTGGGC GGTTTCCACG AGCGTGGCCA AGACTGGGAG 
    CAGAATCAGA AAATCTACAA TTGTCATGTG CTTTTGAACA GCAAGGGATC AGTAGTGGCC 
    AGTTACAGGA AGACACATCT GTGCGATGTA GAGATCCCAG GTCAGGGGCC GATGAGAGAA 
    AGCAACTATA CCAAGCCTGG AGGCACTCTT GAGCCACCTG TCAAGACACC GGCTGGCAAG 
    GTTGGTCTAG CAATCTGTTA TGACATGCGG TTCCCTGAAC TTTCTTTGAA ATTGGCTCAA 
    GCTGGGGCAG AAATACTTAC TTATCCTTCA GCCTTTGGAT CTGTTACAGG TCCAGCCCAC 
    TGGGAGGTGC TGCTGCGGGC CCGCGCCATT GAATCTCAGT GCTATGTAAT AGCAGCAGCG 
    CAGTGTGGAC GCCACCATGA AACAAGAGCA AGTTATGGCC ATAGCATGGT GGTTGACCCG 
    TGGGGCACAG TGGTGGCCCG CTGCTCCGAG GGACCAGGCC TCTGCCTTGC TCGAATTGAT 
    CTCCACTTTC TACAACAGAT GCGCCAACAC CTGCCTGTGT TCCAGCACCG CAGACCTGAC 
    CTCTATGGCA GTCTGGGTCA TCCACTCTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229509.1
    CDS208..1080
    Translation

    Target ORF information:

    RefSeq Version NM_001242580.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitrilase 1 (Nit1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001242580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCCTCAT CAACTTCCTG GGAGCTGCCC CTGGTGGCTG TGTGCCAGGT AACATCAACA 
    CCAAACAAGC AAGAGAACTT TAAAACATGT GCTGAGTTGG TTCAAGAGGC TGCCAGACTG 
    GGTGCTTGCC TGGCCTTTCT GCCTGAGGCA TTTGACTTTA TTGCACGAAA CCCTGCCGAG 
    ACATTACTCC TGTCCGAACC ACTGAATGGG GATCTTTTGG GCCAATATAG CCAGCTTGCC 
    AGGGAATGTG GAATCTGGCT GTCCTTGGGC GGTTTCCACG AGCGTGGCCA AGACTGGGAG 
    CAGAATCAGA AAATCTACAA TTGTCATGTG CTTTTGAACA GCAAGGGATC AGTAGTGGCC 
    AGTTACAGGA AGACACATCT GTGCGATGTA GAGATCCCAG GTCAGGGGCC GATGAGAGAA 
    AGCAACTATA CCAAGCCTGG AGGCACTCTT GAGCCACCTG TCAAGACACC GGCTGGCAAG 
    GTTGGTCTAG CAATCTGTTA TGACATGCGG TTCCCTGAAC TTTCTTTGAA ATTGGCTCAA 
    GCTGGGGCAG AAATACTTAC TTATCCTTCA GCCTTTGGAT CTGTTACAGG TCCAGCCCAC 
    TGGGAGGTGC TGCTGCGGGC CCGCGCCATT GAATCTCAGT GCTATGTAAT AGCAGCAGCG 
    CAGTGTGGAC GCCACCATGA AACAAGAGCA AGTTATGGCC ATAGCATGGT GGTTGACCCG 
    TGGGGCACAG TGGTGGCCCG CTGCTCCGAG GGACCAGGCC TCTGCCTTGC TCGAATTGAT 
    CTCCACTTTC TACAACAGAT GCGCCAACAC CTGCCTGTGT TCCAGCACCG CAGACCTGAC 
    CTCTATGGCA GTCTGGGTCA TCCACTCTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nit1 ( NM_012049.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036179.1 Mus musculus nitrilase 1 (Nit1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OMu09387
    Clone ID Related Accession (Same CDS sequence) NM_012049.2
    Accession Version NM_012049.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Mus musculus(house mouse)
    Product deaminated glutathione amidase isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087229.27 and AC084821.26. On Jun 15, 2010 this sequence version replaced NM_012049.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF069988.1, BC021634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGGGCT TCATCACCAG GCCTCCTCAC CAACTCCTGT GTACCGGATA CCGATTACTT 
    CGAACCCCAG TACTTTGTAC TCAGCCCAGG CCCAGAACCA TGTCCTCATC AACTTCCTGG 
    GAGCTGCCCC TGGTGGCTGT GTGCCAGGTA ACATCAACAC CAAACAAGCA AGAGAACTTT 
    AAAACATGTG CTGAGTTGGT TCAAGAGGCT GCCAGACTGG GTGCTTGCCT GGCCTTTCTG 
    CCTGAGGCAT TTGACTTTAT TGCACGAAAC CCTGCCGAGA CATTACTCCT GTCCGAACCA 
    CTGAATGGGG ATCTTTTGGG CCAATATAGC CAGCTTGCCA GGGAATGTGG AATCTGGCTG 
    TCCTTGGGCG GTTTCCACGA GCGTGGCCAA GACTGGGAGC AGAATCAGAA AATCTACAAT 
    TGTCATGTGC TTTTGAACAG CAAGGGATCA GTAGTGGCCA GTTACAGGAA GACACATCTG 
    TGCGATGTAG AGATCCCAGG TCAGGGGCCG ATGAGAGAAA GCAACTATAC CAAGCCTGGA 
    GGCACTCTTG AGCCACCTGT CAAGACACCG GCTGGCAAGG TTGGTCTAGC AATCTGTTAT 
    GACATGCGGT TCCCTGAACT TTCTTTGAAA TTGGCTCAAG CTGGGGCAGA AATACTTACT 
    TATCCTTCAG CCTTTGGATC TGTTACAGGT CCAGCCCACT GGGAGGTGCT GCTGCGGGCC 
    CGCGCCATTG AATCTCAGTG CTATGTAATA GCAGCAGCGC AGTGTGGACG CCACCATGAA 
    ACAAGAGCAA GTTATGGCCA TAGCATGGTG GTTGACCCGT GGGGCACAGT GGTGGCCCGC 
    TGCTCCGAGG GACCAGGCCT CTGCCTTGCT CGAATTGATC TCCACTTTCT ACAACAGATG 
    CGCCAACACC TGCCTGTGTT CCAGCACCGC AGACCTGACC TCTATGGCAG TCTGGGTCAT 
    CCACTCTCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036179.1
    CDS77..1048
    Translation

    Target ORF information:

    RefSeq Version NM_012049.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitrilase 1 (Nit1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_012049.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGGGCT TCATCACCAG GCCTCCTCAC CAACTCCTGT GTACCGGATA CCGATTACTT 
    CGAACCCCAG TACTTTGTAC TCAGCCCAGG CCCAGAACCA TGTCCTCATC AACTTCCTGG 
    GAGCTGCCCC TGGTGGCTGT GTGCCAGGTA ACATCAACAC CAAACAAGCA AGAGAACTTT 
    AAAACATGTG CTGAGTTGGT TCAAGAGGCT GCCAGACTGG GTGCTTGCCT GGCCTTTCTG 
    CCTGAGGCAT TTGACTTTAT TGCACGAAAC CCTGCCGAGA CATTACTCCT GTCCGAACCA 
    CTGAATGGGG ATCTTTTGGG CCAATATAGC CAGCTTGCCA GGGAATGTGG AATCTGGCTG 
    TCCTTGGGCG GTTTCCACGA GCGTGGCCAA GACTGGGAGC AGAATCAGAA AATCTACAAT 
    TGTCATGTGC TTTTGAACAG CAAGGGATCA GTAGTGGCCA GTTACAGGAA GACACATCTG 
    TGCGATGTAG AGATCCCAGG TCAGGGGCCG ATGAGAGAAA GCAACTATAC CAAGCCTGGA 
    GGCACTCTTG AGCCACCTGT CAAGACACCG GCTGGCAAGG TTGGTCTAGC AATCTGTTAT 
    GACATGCGGT TCCCTGAACT TTCTTTGAAA TTGGCTCAAG CTGGGGCAGA AATACTTACT 
    TATCCTTCAG CCTTTGGATC TGTTACAGGT CCAGCCCACT GGGAGGTGCT GCTGCGGGCC 
    CGCGCCATTG AATCTCAGTG CTATGTAATA GCAGCAGCGC AGTGTGGACG CCACCATGAA 
    ACAAGAGCAA GTTATGGCCA TAGCATGGTG GTTGACCCGT GGGGCACAGT GGTGGCCCGC 
    TGCTCCGAGG GACCAGGCCT CTGCCTTGCT CGAATTGATC TCCACTTTCT ACAACAGATG 
    CGCCAACACC TGCCTGTGTT CCAGCACCGC AGACCTGACC TCTATGGCAG TCTGGGTCAT 
    CCACTCTCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nit1 ( XM_006496866.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496929.1 Mus musculus nitrilase 1 (Nit1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu03250
    Clone ID Related Accession (Same CDS sequence) XM_006496866.3 , NM_001242580.1 , XM_006496866.4
    Accession Version XM_006496866.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitrilase homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCCTCAT CAACTTCCTG GGAGCTGCCC CTGGTGGCTG TGTGCCAGGT AACATCAACA 
    CCAAACAAGC AAGAGAACTT TAAAACATGT GCTGAGTTGG TTCAAGAGGC TGCCAGACTG 
    GGTGCTTGCC TGGCCTTTCT GCCTGAGGCA TTTGACTTTA TTGCACGAAA CCCTGCCGAG 
    ACATTACTCC TGTCCGAACC ACTGAATGGG GATCTTTTGG GCCAATATAG CCAGCTTGCC 
    AGGGAATGTG GAATCTGGCT GTCCTTGGGC GGTTTCCACG AGCGTGGCCA AGACTGGGAG 
    CAGAATCAGA AAATCTACAA TTGTCATGTG CTTTTGAACA GCAAGGGATC AGTAGTGGCC 
    AGTTACAGGA AGACACATCT GTGCGATGTA GAGATCCCAG GTCAGGGGCC GATGAGAGAA 
    AGCAACTATA CCAAGCCTGG AGGCACTCTT GAGCCACCTG TCAAGACACC GGCTGGCAAG 
    GTTGGTCTAG CAATCTGTTA TGACATGCGG TTCCCTGAAC TTTCTTTGAA ATTGGCTCAA 
    GCTGGGGCAG AAATACTTAC TTATCCTTCA GCCTTTGGAT CTGTTACAGG TCCAGCCCAC 
    TGGGAGGTGC TGCTGCGGGC CCGCGCCATT GAATCTCAGT GCTATGTAAT AGCAGCAGCG 
    CAGTGTGGAC GCCACCATGA AACAAGAGCA AGTTATGGCC ATAGCATGGT GGTTGACCCG 
    TGGGGCACAG TGGTGGCCCG CTGCTCCGAG GGACCAGGCC TCTGCCTTGC TCGAATTGAT 
    CTCCACTTTC TACAACAGAT GCGCCAACAC CTGCCTGTGT TCCAGCACCG CAGACCTGAC 
    CTCTATGGCA GTCTGGGTCA TCCACTCTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496929.1
    CDS499..1371
    Translation

    Target ORF information:

    RefSeq Version XM_006496866.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitrilase 1 (Nit1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006496866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCCTCAT CAACTTCCTG GGAGCTGCCC CTGGTGGCTG TGTGCCAGGT AACATCAACA 
    CCAAACAAGC AAGAGAACTT TAAAACATGT GCTGAGTTGG TTCAAGAGGC TGCCAGACTG 
    GGTGCTTGCC TGGCCTTTCT GCCTGAGGCA TTTGACTTTA TTGCACGAAA CCCTGCCGAG 
    ACATTACTCC TGTCCGAACC ACTGAATGGG GATCTTTTGG GCCAATATAG CCAGCTTGCC 
    AGGGAATGTG GAATCTGGCT GTCCTTGGGC GGTTTCCACG AGCGTGGCCA AGACTGGGAG 
    CAGAATCAGA AAATCTACAA TTGTCATGTG CTTTTGAACA GCAAGGGATC AGTAGTGGCC 
    AGTTACAGGA AGACACATCT GTGCGATGTA GAGATCCCAG GTCAGGGGCC GATGAGAGAA 
    AGCAACTATA CCAAGCCTGG AGGCACTCTT GAGCCACCTG TCAAGACACC GGCTGGCAAG 
    GTTGGTCTAG CAATCTGTTA TGACATGCGG TTCCCTGAAC TTTCTTTGAA ATTGGCTCAA 
    GCTGGGGCAG AAATACTTAC TTATCCTTCA GCCTTTGGAT CTGTTACAGG TCCAGCCCAC 
    TGGGAGGTGC TGCTGCGGGC CCGCGCCATT GAATCTCAGT GCTATGTAAT AGCAGCAGCG 
    CAGTGTGGAC GCCACCATGA AACAAGAGCA AGTTATGGCC ATAGCATGGT GGTTGACCCG 
    TGGGGCACAG TGGTGGCCCG CTGCTCCGAG GGACCAGGCC TCTGCCTTGC TCGAATTGAT 
    CTCCACTTTC TACAACAGAT GCGCCAACAC CTGCCTGTGT TCCAGCACCG CAGACCTGAC 
    CTCTATGGCA GTCTGGGTCA TCCACTCTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nit1 ( XM_006496866.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496929.1 Mus musculus nitrilase 1 (Nit1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu03250
    Clone ID Related Accession (Same CDS sequence) XM_006496866.3 , NM_001242580.1 , XM_006496866.4
    Accession Version XM_006496866.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product deaminated glutathione amidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496866.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCCTCAT CAACTTCCTG GGAGCTGCCC CTGGTGGCTG TGTGCCAGGT AACATCAACA 
    CCAAACAAGC AAGAGAACTT TAAAACATGT GCTGAGTTGG TTCAAGAGGC TGCCAGACTG 
    GGTGCTTGCC TGGCCTTTCT GCCTGAGGCA TTTGACTTTA TTGCACGAAA CCCTGCCGAG 
    ACATTACTCC TGTCCGAACC ACTGAATGGG GATCTTTTGG GCCAATATAG CCAGCTTGCC 
    AGGGAATGTG GAATCTGGCT GTCCTTGGGC GGTTTCCACG AGCGTGGCCA AGACTGGGAG 
    CAGAATCAGA AAATCTACAA TTGTCATGTG CTTTTGAACA GCAAGGGATC AGTAGTGGCC 
    AGTTACAGGA AGACACATCT GTGCGATGTA GAGATCCCAG GTCAGGGGCC GATGAGAGAA 
    AGCAACTATA CCAAGCCTGG AGGCACTCTT GAGCCACCTG TCAAGACACC GGCTGGCAAG 
    GTTGGTCTAG CAATCTGTTA TGACATGCGG TTCCCTGAAC TTTCTTTGAA ATTGGCTCAA 
    GCTGGGGCAG AAATACTTAC TTATCCTTCA GCCTTTGGAT CTGTTACAGG TCCAGCCCAC 
    TGGGAGGTGC TGCTGCGGGC CCGCGCCATT GAATCTCAGT GCTATGTAAT AGCAGCAGCG 
    CAGTGTGGAC GCCACCATGA AACAAGAGCA AGTTATGGCC ATAGCATGGT GGTTGACCCG 
    TGGGGCACAG TGGTGGCCCG CTGCTCCGAG GGACCAGGCC TCTGCCTTGC TCGAATTGAT 
    CTCCACTTTC TACAACAGAT GCGCCAACAC CTGCCTGTGT TCCAGCACCG CAGACCTGAC 
    CTCTATGGCA GTCTGGGTCA TCCACTCTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496929.1
    CDS564..1436
    Translation

    Target ORF information:

    RefSeq Version XM_006496866.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitrilase 1 (Nit1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006496866.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCCTCAT CAACTTCCTG GGAGCTGCCC CTGGTGGCTG TGTGCCAGGT AACATCAACA 
    CCAAACAAGC AAGAGAACTT TAAAACATGT GCTGAGTTGG TTCAAGAGGC TGCCAGACTG 
    GGTGCTTGCC TGGCCTTTCT GCCTGAGGCA TTTGACTTTA TTGCACGAAA CCCTGCCGAG 
    ACATTACTCC TGTCCGAACC ACTGAATGGG GATCTTTTGG GCCAATATAG CCAGCTTGCC 
    AGGGAATGTG GAATCTGGCT GTCCTTGGGC GGTTTCCACG AGCGTGGCCA AGACTGGGAG 
    CAGAATCAGA AAATCTACAA TTGTCATGTG CTTTTGAACA GCAAGGGATC AGTAGTGGCC 
    AGTTACAGGA AGACACATCT GTGCGATGTA GAGATCCCAG GTCAGGGGCC GATGAGAGAA 
    AGCAACTATA CCAAGCCTGG AGGCACTCTT GAGCCACCTG TCAAGACACC GGCTGGCAAG 
    GTTGGTCTAG CAATCTGTTA TGACATGCGG TTCCCTGAAC TTTCTTTGAA ATTGGCTCAA 
    GCTGGGGCAG AAATACTTAC TTATCCTTCA GCCTTTGGAT CTGTTACAGG TCCAGCCCAC 
    TGGGAGGTGC TGCTGCGGGC CCGCGCCATT GAATCTCAGT GCTATGTAAT AGCAGCAGCG 
    CAGTGTGGAC GCCACCATGA AACAAGAGCA AGTTATGGCC ATAGCATGGT GGTTGACCCG 
    TGGGGCACAG TGGTGGCCCG CTGCTCCGAG GGACCAGGCC TCTGCCTTGC TCGAATTGAT 
    CTCCACTTTC TACAACAGAT GCGCCAACAC CTGCCTGTGT TCCAGCACCG CAGACCTGAC 
    CTCTATGGCA GTCTGGGTCA TCCACTCTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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