Orai3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Orai3
Entrez Gene ID	269999
Full Name	ORAI calcium release-activated calcium modulator 3
Synonyms	9930124N15,BC061259,Tmem142c
General protein information	Preferred Names ORAI calcium release-activated calcium modulator 3 Names protein orai-3 calcium release-activated calcium channel 3 transmembrane protein 142C
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_198424.3	NP_940816.1	protein orai-3
XM_006507902.2	XP_006507965.1	protein orai-3 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ORAI3	XP_003315113.1
Canis lupus familiaris (dog)	ORAI3	XP_849021.1
Mus musculus (house mouse)	Orai3	NP_940816.1
	ORAI3	XP_001111639.1
Homo sapiens (human)	ORAI3	NP_689501.1
Rattus norvegicus (Norway rat)	Orai3	NP_001014046.1
Bos taurus (cattle)	ORAI3	NP_001180131.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005071	XP_001688479.1

Gene Ontology
Bibliography

Related articles in PubMed

ORAI2 modulates store-operated calcium entry and T cell-mediated immunity.
Vaeth M, Yang J, Yamashita M, Zee I, Eckstein M, Knosp C, Kaufmann U, Karoly Jani P, Lacruz RS, Flockerzi V, Kacskovics I, Prakriya M, Feske S
Nature communications814714(2017 Mar)

The role of STIM and ORAI proteins in phagocytic immune cells.
Demaurex N, Nunes P
American journal of physiology. Cell physiology310(7)C496-508(2016 Apr)

A calcium-redox feedback loop controls human monocyte immune responses: The role of ORAI Ca2+ channels.
Saul S, Gibhardt CS, Schmidt B, Lis A, Pasieka B, Conrad D, Jung P, Gaupp R, Wonnenberg B, Diler E, Stanisz H, Vogt T, Schwarz EC, Bischoff M, Herrmann M, Tschernig T, Kappl R, Rieger H, Niemeyer BA, Bogeski I
Science signaling9(418)ra26(2016 Mar)

Orai3 Surface Accumulation and Calcium Entry Evoked by Vascular Endothelial Growth Factor.
Li J, Bruns AF, Hou B, Rode B, Webster PJ, Bailey MA, Appleby HL, Moss NK, Ritchie JE, Yuldasheva NY, Tumova S, Quinney M, McKeown L, Taylor H, Prasad KR, Burke D, O'Regan D, Porter KE, Foster R, Kearney MT, Beech DJ
Arteriosclerosis, thrombosis, and vascular biology35(9)1987-94(2015 Sep)

Defective mast cell effector functions in mice lacking the CRACM1 pore subunit of store-operated calcium release-activated calcium channels.
Vig M, DeHaven WI, Bird GS, Billingsley JM, Wang H, Rao PE, Hutchings AB, Jouvin MH, Putney JW, Kinet JP
Nature immunology9(1)89-96(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results identified the ORAI-NOX2 feedback loop as a determinant of monocyte immune responses.
Title: A calcium-redox feedback loop controls human monocyte immune responses: The role of ORAI Ca2+ channels.

VEGF signaling via arachidonic acid and arachidonic acid metabolism causes Orai3 to accumulate at the cell surface to mediate Ca(2+) entry and downstream endothelial cell remodeling.
Title: Orai3 Surface Accumulation and Calcium Entry Evoked by Vascular Endothelial Growth Factor.

The following Orai3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Orai3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09022	NM_198424.3 Latest version!	Mus musculus ORAI calcium release-activated calcium modulator 3 (Orai3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu39690	XM_006507902.2 Latest version!	Mus musculus ORAI calcium release-activated calcium modulator 3 (Orai3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Orai3 ( NM_198424.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_940816.1 Mus musculus ORAI calcium release-activated calcium modulator 3 (Orai3), mRNA.

Price & Availability↑

CloneID	OMu09022
Clone ID Related Accession (Same CDS sequence)	NM_198424.3
Accession Version	NM_198424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	protein orai-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK136955.1. On Apr 5, 2007 this sequence version replaced NM_198424.2. ##Evidence-Data-START## Transcript exon combination :: AK136955.1, BC061259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGGCG GCGAGGGGGA CACGGGCGAA CAGGCCCCGC TGAACCCGGA GGTGGACAGC 
CCCGCGGGCT CAGCCACGTA CCGGGAGTTC GTGCACCGCG GCTACCTGGA CCTTATGGGG 
GCCAGTCAGC ACTCTCTGCG GGCGCTCAGC TGGCGCCGCC TCTACCTCAG CCGGGCTAAG 
CTCAAAGCCT CCAGCCGCAC ATCTGCTCTG CTGTCGGGCT TCGCCATGGT GGCCATGGTG 
GAGGTACAGC TGGAGAACGA TCATGAATAC CCACCAGGCC TGCTGGTGGC ATTTAGTGCC 
TGCACCACTG TGTTAGTAGC TGTGCACCTC TTTGCACTGA TGGTCTCCAC ATGTCTGCTG 
CCCCATATTG AAGCCGTGAG CAACATCCAC AATCTCAACT CTGTCCACCA GTCACCACAC 
CAACGACTGC ACAGATACGT GGAGCTTGCC TGGGGCTTCT CTACTGCCTT GGGTACCTTT 
CTCTTCCTGG CTGAAGTTGT TCTGGTGGGC TGGGTCAAGT TTGTGCCCAT TGGGGCACCC 
ATGGGTAAAC CAGCTCCTGT TGTACCTATG TCCCAGGTGC CACCTGTGAC TGTCTCCCTT 
AGTTTAGCTT CTAACCTCAC ACCATCCTCT GCTTCTATTA CCACATCACA ACAGCCTTCC 
AAAGCCTGTC CACCCCGGCA AGTGTGTGAT AGTGCTCATG GACCAGGCTG GCAGGCAGCT 
ATGGCCTCCA CGGCAATCAT GGTACCTGTG GGACTAGTGT TTATGGCCTT TGCCCTACAT 
TTCTACCGAT CCTTGGTTGC CCACAAGACA GACCGCCACA AGCAGGAGCT GGAGGAGCTC 
AGTCGCCTGC AGGGGGAGCT GCAGGCTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940816.1
CDS	175..1047
Translation	MKGGEGDTGEQAPLNPEVDSPAGSATYREFVHRGYLDLMGASQHSLRALSWRRLYLSRAKLKASSRTSALLSGFAMVAMVEVQLENDHEYPPGLLVAFSACTTVLVAVHLFALMVSTCLLPHIEAVSNIHNLNSVHQSPHQRLHRYVELAWGFSTALGTFLFLAEVVLVGWVKFVPIGAPMGKPAPVVPMSQVPPVTVSLSLASNLTPSSASITTSQQPSKACPPRQVCDSAHGPGWQAAMASTAIMVPVGLVFMAFALHFYRSLVAHKTDRHKQELEELSRLQGELQAV

Target ORF information:

RefSeq Version	NM_198424.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ORAI calcium release-activated calcium modulator 3 (Orai3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198424.3

ORF Insert Sequence:

ATGAAGGGCG GCGAGGGGGA CACGGGCGAA CAGGCCCCGC TGAACCCGGA GGTGGACAGC 
CCCGCGGGCT CAGCCACGTA CCGGGAGTTC GTGCACCGCG GCTACCTGGA CCTTATGGGG 
GCCAGTCAGC ACTCTCTGCG GGCGCTCAGC TGGCGCCGCC TCTACCTCAG CCGGGCTAAG 
CTCAAAGCCT CCAGCCGCAC ATCTGCTCTG CTGTCGGGCT TCGCCATGGT GGCCATGGTG 
GAGGTACAGC TGGAGAACGA TCATGAATAC CCACCAGGCC TGCTGGTGGC ATTTAGTGCC 
TGCACCACTG TGTTAGTAGC TGTGCACCTC TTTGCACTGA TGGTCTCCAC ATGTCTGCTG 
CCCCATATTG AAGCCGTGAG CAACATCCAC AATCTCAACT CTGTCCACCA GTCACCACAC 
CAACGACTGC ACAGATACGT GGAGCTTGCC TGGGGCTTCT CTACTGCCTT GGGTACCTTT 
CTCTTCCTGG CTGAAGTTGT TCTGGTGGGC TGGGTCAAGT TTGTGCCCAT TGGGGCACCC 
ATGGGTAAAC CAGCTCCTGT TGTACCTATG TCCCAGGTGC CACCTGTGAC TGTCTCCCTT 
AGTTTAGCTT CTAACCTCAC ACCATCCTCT GCTTCTATTA CCACATCACA ACAGCCTTCC 
AAAGCCTGTC CACCCCGGCA AGTGTGTGAT AGTGCTCATG GACCAGGCTG GCAGGCAGCT 
ATGGCCTCCA CGGCAATCAT GGTACCTGTG GGACTAGTGT TTATGGCCTT TGCCCTACAT 
TTCTACCGAT CCTTGGTTGC CCACAAGACA GACCGCCACA AGCAGGAGCT GGAGGAGCTC 
AGTCGCCTGC AGGGGGAGCT GCAGGCTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Orai3 ( XM_006507902.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507965.1 Mus musculus ORAI calcium release-activated calcium modulator 3 (Orai3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39690
Clone ID Related Accession (Same CDS sequence)	XM_006507902.2
Accession Version	XM_006507902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein orai-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGG TACAGCTGGA GAACGATCAT GAATACCCAC CAGGCCTGCT GGTGGCATTT 
AGTGCCTGCA CCACTGTGTT AGTAGCTGTG CACCTCTTTG CACTGATGGT CTCCACATGT 
CTGCTGCCCC ATATTGAAGC CGTGAGCAAC ATCCACAATC TCAACTCTGT CCACCAGTCA 
CCACACCAAC GACTGCACAG ATACGTGGAG CTTGCCTGGG GCTTCTCTAC TGCCTTGGGT 
ACCTTTCTCT TCCTGGCTGA AGTTGTTCTG GTGGGCTGGG TCAAGTTTGT GCCCATTGGG 
GCACCCATGG GTAAACCAGC TCCTGTTGTA CCTATGTCCC AGGTGCCACC TGTGACTGTC 
TCCCTTAGTT TAGCTTCTAA CCTCACACCA TCCTCTGCTT CTATTACCAC ATCACAACAG 
CCTTCCAAAG CCTGTCCACC CCGGCAAGTG TGTGATAGTG CTCATGGACC AGGCTGGCAG 
GCAGCTATGG CCTCCACGGC AATCATGGTA CCTGTGGGAC TAGTGTTTAT GGCCTTTGCC 
CTACATTTCT ACCGATCCTT GGTTGCCCAC AAGACAGACC GCCACAAGCA GGAGCTGGAG 
GAGCTCAGTC GCCTGCAGGG GGAGCTGCAG GCTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507965.1
CDS	31..669
Translation	MVEVQLENDHEYPPGLLVAFSACTTVLVAVHLFALMVSTCLLPHIEAVSNIHNLNSVHQSPHQRLHRYVELAWGFSTALGTFLFLAEVVLVGWVKFVPIGAPMGKPAPVVPMSQVPPVTVSLSLASNLTPSSASITTSQQPSKACPPRQVCDSAHGPGWQAAMASTAIMVPVGLVFMAFALHFYRSLVAHKTDRHKQELEELSRLQGELQAV

Target ORF information:

RefSeq Version	XM_006507902.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ORAI calcium release-activated calcium modulator 3 (Orai3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507902.2

ORF Insert Sequence:

ATGGTGGAGG TACAGCTGGA GAACGATCAT GAATACCCAC CAGGCCTGCT GGTGGCATTT 
AGTGCCTGCA CCACTGTGTT AGTAGCTGTG CACCTCTTTG CACTGATGGT CTCCACATGT 
CTGCTGCCCC ATATTGAAGC CGTGAGCAAC ATCCACAATC TCAACTCTGT CCACCAGTCA 
CCACACCAAC GACTGCACAG ATACGTGGAG CTTGCCTGGG GCTTCTCTAC TGCCTTGGGT 
ACCTTTCTCT TCCTGGCTGA AGTTGTTCTG GTGGGCTGGG TCAAGTTTGT GCCCATTGGG 
GCACCCATGG GTAAACCAGC TCCTGTTGTA CCTATGTCCC AGGTGCCACC TGTGACTGTC 
TCCCTTAGTT TAGCTTCTAA CCTCACACCA TCCTCTGCTT CTATTACCAC ATCACAACAG 
CCTTCCAAAG CCTGTCCACC CCGGCAAGTG TGTGATAGTG CTCATGGACC AGGCTGGCAG 
GCAGCTATGG CCTCCACGGC AATCATGGTA CCTGTGGGAC TAGTGTTTAT GGCCTTTGCC 
CTACATTTCT ACCGATCCTT GGTTGCCCAC AAGACAGACC GCCACAAGCA GGAGCTGGAG 
GAGCTCAGTC GCCTGCAGGG GGAGCTGCAG GCTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ORAI2 modulates store-operated calcium entry and T cell-mediated immunity.
Nature communications814714(2017 Mar)
Vaeth M,Yang J,Yamashita M,Zee I,Eckstein M,Knosp C,Kaufmann U,Karoly Jani P,Lacruz RS,Flockerzi V,Kacskovics I,Prakriya M,Feske S

The role of STIM and ORAI proteins in phagocytic immune cells.
American journal of physiology. Cell physiology310(7)C496-508(2016 Apr)
Demaurex N,Nunes P

A calcium-redox feedback loop controls human monocyte immune responses: The role of ORAI Ca2+ channels.
Science signaling9(418)ra26(2016 Mar)
Saul S,Gibhardt CS,Schmidt B,Lis A,Pasieka B,Conrad D,Jung P,Gaupp R,Wonnenberg B,Diler E,Stanisz H,Vogt T,Schwarz EC,Bischoff M,Herrmann M,Tschernig T,Kappl R,Rieger H,Niemeyer BA,Bogeski I

Orai3 Surface Accumulation and Calcium Entry Evoked by Vascular Endothelial Growth Factor.
Arteriosclerosis, thrombosis, and vascular biology35(9)1987-94(2015 Sep)
Li J,Bruns AF,Hou B,Rode B,Webster PJ,Bailey MA,Appleby HL,Moss NK,Ritchie JE,Yuldasheva NY,Tumova S,Quinney M,McKeown L,Taylor H,Prasad KR,Burke D,O'Regan D,Porter KE,Foster R,Kearney MT,Beech DJ

Defective mast cell effector functions in mice lacking the CRACM1 pore subunit of store-operated calcium release-activated calcium channels.
Nature immunology9(1)89-96(2008 Jan)
Vig M,DeHaven WI,Bird GS,Billingsley JM,Wang H,Rao PE,Hutchings AB,Jouvin MH,Putney JW,Kinet JP