Mrgprx2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrgprx2
Entrez Gene ID	243978
Full Name	MAS-related GPR, member X2
Synonyms	G370024M05Rik,MrgB10,Mrgprb10
General protein information	Preferred Names MAS-related GPR, member X2 Names mas-related G-protein coupled receptor member X2 MAS-related GPR, member B10 mas-related G-protein coupled receptor member B10
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034868.3	NP_001030040.2	mas-related G-protein coupled receptor member X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MRGPRX2	XP_521864.3
Homo sapiens (human)	MRGPRX2	NP_473371.1
	MRGPRX2	NP_001035512.1
Bos taurus (cattle)	MRGPRX2	NP_001179379.1
Mus musculus (house mouse)	Mrgprb1	NP_991379.3
Canis lupus familiaris (dog)	MRGPRX2	XP_005633869.1
Rattus norvegicus (Norway rat)	Mrgprx2	NP_001002280.1
Mus musculus (house mouse)	Mrgprx2	NP_001030040.2

Gene Ontology
Bibliography

Related articles in PubMed

MRGPRX2 is essential for sinomenine hydrochloride induced anaphylactoid reactions.
Liu R, Che D, Zhao T, Pundir P, Cao J, Lv Y, Wang J, Ma P, Fu J, Wang N, Wang X, Zhang T, Dong X, He L
Biochemical pharmacology146214-223(2017 Dec)

Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions.
McNeil BD, Pundir P, Meeker S, Han L, Undem BJ, Kulka M, Dong X
Nature519(7542)237-41(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results showed a kind of mechanism that sinomenine-induced anaphylactoid reactions were mediated by MRGPRX2 via activating PLC molecular signaling pathways to provoke mast cells Ca(2+) mobilization and degranulation.
Title: MRGPRX2 is essential for sinomenine hydrochloride induced anaphylactoid reactions.

The following Mrgprx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrgprx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12779	NM_001034868.3 Latest version!	Mus musculus MAS-related GPR, member X2 (Mrgprx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrgprx2 ( NM_001034868.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001030040.2 Mus musculus MAS-related GPR, member X2 (Mrgprx2), mRNA.

Price & Availability↑

CloneID	OMu12779
Clone ID Related Accession (Same CDS sequence)	NM_001034868.3
Accession Version	NM_001034868.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mas-related G-protein coupled receptor member X2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ131827.1, AK148121.1, AK148319.1 and AC115742.13. On May 20, 2008 this sequence version replaced NM_001034868.2. ##Evidence-Data-START## Transcript exon combination :: AK148121.1, BC141225.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAAA GAAACATCAG TGGAAGGGAT CTAAGAGTGG ATTCAAACAT AACATACTGG 
GGAACAAACA TCACAGCTGT GAATGAAAGC AACCATACTG GAATGTCATT TTGTGAAGTC 
GTGTCTTGTA CCATGGTTTT TCTTTCCCTC ATTGTTGCCC TAGTTGGGCT GGTTGGAAAT 
GCCACAGTGC TGTGGTTCCT GGGCTTCCAG ATGCGCAGGA ATGCATTCTC TGTTTACATC 
CTCAACCTCG CTGGTGCTGA CTTTCTCTTC ATTTGCTTTC AAATTGGATA TTGTTTTCAC 
ATGATCTTGG ACATTGATTC CATCCCCATT GAAATTGATC TGTTTTACCT TGTTGTGTTA 
AACTTTCCTT ATTTTTGTGG CCTGAGTATC CTCAGTGCTA TTAGCATTGA ACGTTGCCTG 
TCTGTCATGT GGCCCATTTG GTATCACTGC CAACGCCCAA GGCACACATC AGCTGTCATA 
TGTACCCTGC TTTGGGTCTT GTCCCTAGTG TGTAGCCTCC TGGAAGGGAA GGAATGTGGC 
TTCCTATATT ACACTAGTGA CCCTGGTTGG TGTAAGACAT TTGATTTAAT CACTGCTACA 
TGGTTAATTG TTTTATTTGT AGCTCTCTTG GGATCCAGTC TGGCCTTAGT GATTACCATC 
TTCTGGGGCT TACACAAGAT TCCTGTGACC AGGCTGTATG TGGCCATTGT GTTCACAGTG 
CTTGTTTTCC TGCTCTTTGG TCTGCCCTAT GGGATCTACT GGTTCCTCTT AGTGTGGATT 
GAGAAATTTT ATTATGTTTT ACCTTGTAGT ATATATCCGG TCACAGTATT TCTCTCCTGT 
GTTAACAGCT CTGCAAAACC CATCATTTAT TGCCTTGTAG GCTCCATTAG GCATCATCGA 
TTTCAACGGA AGACTCTCAA GCTATTTCTG CAGAGAGCCA TGCAAGACAC TCCTGAGGAG 
GAAGAATGTG GAGAGATGGG TTCCTCAGGA AGATCTAGAG AAATAAAAAC AATCTGGAAA 
GGACTGAGAG CTGCTTTGAT CAGGCATAAA GAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030040.2
CDS	110..1168
Translation	MEERNISGRDLRVDSNITYWGTNITAVNESNHTGMSFCEVVSCTMVFLSLIVALVGLVGNATVLWFLGFQMRRNAFSVYILNLAGADFLFICFQIGYCFHMILDIDSIPIEIDLFYLVVLNFPYFCGLSILSAISIERCLSVMWPIWYHCQRPRHTSAVICTLLWVLSLVCSLLEGKECGFLYYTSDPGWCKTFDLITATWLIVLFVALLGSSLALVITIFWGLHKIPVTRLYVAIVFTVLVFLLFGLPYGIYWFLLVWIEKFYYVLPCSIYPVTVFLSCVNSSAKPIIYCLVGSIRHHRFQRKTLKLFLQRAMQDTPEEEECGEMGSSGRSREIKTIWKGLRAALIRHKEL

Target ORF information:

RefSeq Version	NM_001034868.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAS-related GPR, member X2 (Mrgprx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034868.3

ORF Insert Sequence:

ATGGAGGAAA GAAACATCAG TGGAAGGGAT CTAAGAGTGG ATTCAAACAT AACATACTGG 
GGAACAAACA TCACAGCTGT GAATGAAAGC AACCATACTG GAATGTCATT TTGTGAAGTC 
GTGTCTTGTA CCATGGTTTT TCTTTCCCTC ATTGTTGCCC TAGTTGGGCT GGTTGGAAAT 
GCCACAGTGC TGTGGTTCCT GGGCTTCCAG ATGCGCAGGA ATGCATTCTC TGTTTACATC 
CTCAACCTCG CTGGTGCTGA CTTTCTCTTC ATTTGCTTTC AAATTGGATA TTGTTTTCAC 
ATGATCTTGG ACATTGATTC CATCCCCATT GAAATTGATC TGTTTTACCT TGTTGTGTTA 
AACTTTCCTT ATTTTTGTGG CCTGAGTATC CTCAGTGCTA TTAGCATTGA ACGTTGCCTG 
TCTGTCATGT GGCCCATTTG GTATCACTGC CAACGCCCAA GGCACACATC AGCTGTCATA 
TGTACCCTGC TTTGGGTCTT GTCCCTAGTG TGTAGCCTCC TGGAAGGGAA GGAATGTGGC 
TTCCTATATT ACACTAGTGA CCCTGGTTGG TGTAAGACAT TTGATTTAAT CACTGCTACA 
TGGTTAATTG TTTTATTTGT AGCTCTCTTG GGATCCAGTC TGGCCTTAGT GATTACCATC 
TTCTGGGGCT TACACAAGAT TCCTGTGACC AGGCTGTATG TGGCCATTGT GTTCACAGTG 
CTTGTTTTCC TGCTCTTTGG TCTGCCCTAT GGGATCTACT GGTTCCTCTT AGTGTGGATT 
GAGAAATTTT ATTATGTTTT ACCTTGTAGT ATATATCCGG TCACAGTATT TCTCTCCTGT 
GTTAACAGCT CTGCAAAACC CATCATTTAT TGCCTTGTAG GCTCCATTAG GCATCATCGA 
TTTCAACGGA AGACTCTCAA GCTATTTCTG CAGAGAGCCA TGCAAGACAC TCCTGAGGAG 
GAAGAATGTG GAGAGATGGG TTCCTCAGGA AGATCTAGAG AAATAAAAAC AATCTGGAAA 
GGACTGAGAG CTGCTTTGAT CAGGCATAAA GAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MRGPRX2 is essential for sinomenine hydrochloride induced anaphylactoid reactions.
Biochemical pharmacology146214-223(2017 Dec)
Liu R,Che D,Zhao T,Pundir P,Cao J,Lv Y,Wang J,Ma P,Fu J,Wang N,Wang X,Zhang T,Dong X,He L

Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions.
Nature519(7542)237-41(2015 Mar)
McNeil BD,Pundir P,Meeker S,Han L,Undem BJ,Kulka M,Dong X