Ralgapa2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ralgapa2
Entrez Gene ID	241694
Full Name	Ral GTPase activating protein, alpha subunit 2 (catalytic)
Synonyms	A230067G21Rik,AS250,BC053994,RGC2,pp250
General protein information	Preferred Names Ral GTPase activating protein, alpha subunit 2 (catalytic) Names ral GTPase-activating protein subunit alpha-2 250 kDa substrate of Akt P220 ral GTPase-activating protein alpha subunit 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 G1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499519.4	XP_006499582.1	ral GTPase-activating protein subunit alpha-2 isoform X2
XM_006499517.3	XP_006499580.1	ral GTPase-activating protein subunit alpha-2 isoform X1
XM_006499519.3	XP_006499582.1	ral GTPase-activating protein subunit alpha-2 isoform X2
XM_006499521.3	XP_006499584.1	ral GTPase-activating protein subunit alpha-2 isoform X3
NM_001033348.3	NP_001028520.2	ral GTPase-activating protein subunit alpha-2
XM_006499517.4	XP_006499580.1	ral GTPase-activating protein subunit alpha-2 isoform X1

Pathways from BioSystems

Homologies

	RALGAPA2	XP_001092526.2
Bos taurus (cattle)	RALGAPA2	XP_005214436.1
Rattus norvegicus (Norway rat)	Ralgapa2	XP_006235240.1
Homo sapiens (human)	RALGAPA2	NP_065076.2
Xenopus tropicalis (tropical clawed frog)	ralgapa2	NP_001120298.1
Gallus gallus (chicken)	RALGAPA2	XP_004940197.1
Canis lupus familiaris (dog)	RALGAPA2	XP_005634770.1
Danio rerio (zebrafish)	ralgapa2	XP_005158962.1
Pan troglodytes (chimpanzee)	RALGAPA2	XP_001144701.3
Mus musculus (house mouse)	Ralgapa2	NP_001028520.2

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of Ral GTPase-activating protein promotes tumor invasion and metastasis of bladder cancer.
Saito R, Shirakawa R, Nishiyama H, Kobayashi T, Kawato M, Kanno T, Nishizawa K, Matsui Y, Ohbayashi T, Horiguchi M, Nakamura T, Ikeda T, Yamane K, Nakayama E, Nakamura E, Toda Y, Kimura T, Kita T, Ogawa O, Horiuchi H
Oncogene32(7)894-902(2013 Feb)

A Ral GAP complex links PI 3-kinase/Akt signaling to RalA activation in insulin action.
Chen XW, Leto D, Xiong T, Yu G, Cheng A, Decker S, Saltiel AR
Molecular biology of the cell22(1)141-52(2011 Jan)

Adipocytes contain a novel complex similar to the tuberous sclerosis complex.
Gridley S, Chavez JA, Lane WS, Lienhard GE
Cellular signalling18(10)1626-32(2006 Oct)

Novel insulin-elicited phosphoproteins in adipocytes.
Gridley S, Lane WS, Garner CW, Lienhard GE
Cellular signalling17(1)59-66(2005 Jan)

Transcriptome analysis of mouse stem cells and early embryos.
Sharov AA, Piao Y, Matoba R, Dudekula DB, Qian Y, VanBuren V, Falco G, Martin PR, Stagg CA, Bassey UC, Wang Y, Carter MG, Hamatani T, Aiba K, Akutsu H, Sharova L, Tanaka TS, Kimber WL, Yoshikawa T, Jaradat SA, Pantano S, Nagaraja R, Boheler KR, Taub D, Hodes RJ, Longo DL, Schlessinger D, Keller J, Klotz E, Kelsoe G, Umezawa A, Vescovi AL, Rossant J, Kunath T, Hogan BL, Curci A, D'Urso M, Kelso J, Hide W, Ko MS
PLoS biology1(3)E74(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study reports the identification and characterization of a Ral GAP complex (RG1, RGC2) that mediates the activation of RalA downstream of the PI 3-kinase/Akt pathway
Title: A Ral GAP complex links PI 3-kinase/Akt signaling to RalA activation in insulin action.

The following Ralgapa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ralgapa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30475	XM_006499521.3 Latest version!	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu30472	XM_006499517.4 Latest version!	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu30473	XM_006499519.4 Latest version!	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu15148	NM_001033348.3 Latest version!	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1406.30 $2009.00
View Old Accession Versions >>
OMu30473	XM_006499519.3	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu30472	XM_006499517.3	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ralgapa2 ( XM_006499521.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499584.1 Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu30475
Clone ID Related Accession (Same CDS sequence)	XM_006499521.3
Accession Version	XM_006499521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGATGC ATCGCCATTT GCTCCCTCGG GGTCTGGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499584.1
CDS	329..4033
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATAKRSQSISNCVHLSEALPATKSVPLLLHTVSALLPGLSYSSCSHRLSEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKMHRHLLPRGLDL

Target ORF information:

RefSeq Version	XM_006499521.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499521.3

ORF Insert Sequence:

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGATGC ATCGCCATTT GCTCCCTCGG GGTCTGGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa2 ( XM_006499517.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499580.1 Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu30472
Clone ID Related Accession (Same CDS sequence)	XM_006499517.3 , XM_006499517.4
Accession Version	XM_006499517.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ral GTPase-activating protein subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499517.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGT GAAAGAACAG 
GAGGAATCCA CAGCAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499580.1
CDS	356..6256
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATAKRSQSISNCVHLSEALPATKSVPLLLHTVSALLPGLSYSSCSHRLSEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTVKEQEESTAS

Target ORF information:

RefSeq Version	XM_006499517.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499517.4

ORF Insert Sequence:

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGT GAAAGAACAG 
GAGGAATCCA CAGCAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa2 ( XM_006499519.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499582.1 Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X3, mRNA.

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CloneID	OMu30473
Clone ID Related Accession (Same CDS sequence)	XM_006499519.4 , XM_006499519.3
Accession Version	XM_006499519.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ral GTPase-activating protein subunit alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499519.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499582.1
CDS	356..6229
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATAKRSQSISNCVHLSEALPATKSVPLLLHTVSALLPGLSYSSCSHRLSEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_006499519.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499519.4

ORF Insert Sequence:

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa2 ( NM_001033348.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028520.2 Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), mRNA.

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CloneID	OMu15148
Clone ID Related Accession (Same CDS sequence)	NM_001033348.3
Accession Version	NM_001033348.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	ral GTPase-activating protein subunit alpha-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK157932.1, AK038838.1, CN537732.1, CK638421.1, CN706154.1, AL845273.7, CJ114686.1, BC096528.1, AL935056.18 and CJ251166.1. On Oct 12, 2007 this sequence version replaced NM_001033348.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGAAGTGGA GGAGTTCCAG 
CAGGCAGAGA GCACCGCCGC AGCCGACTGT GACTATCTTG TGGTAGGACA GCAGCAGGTG 
CCCCGGAGCA GCAGCACCTC TGACATCACT GAGCGCTTGT ACTCAGACTC CTCTCAAGGT 
TACAGCCCCA TGTTGCCTCA TCCTGCTTTT TCCACGACTT CTGGGGATCA GAGCTCTGGT 
CCTCATGCTT GCACAGCTGG CCCTTTACCT TTGAGCCGTC TCCCCAGTGC AGGTCAAAAG 
GTAGAACATT CGCAGAATTT AAGCTCTTCA GAACCCAAGT CTGTTCAAGA GAGTAAAGGA 
CATGTGACTC ATGAACATGA AGGAATAACA ATGTTGGTTC GAAGGAGCAG CAGTCCCGCT 
GAGCTGGAGC TGAAGGATGA TTTACAGCAG GCACATGGAA GATGCAGGCA GAGACAGACA 
AGTGAAAGTA CTGGTAGTGA CACAGTTGTG GGCTACAGCA ATGAAGCAGA GCTACCTGTG 
AGCCCGTGGC AGGCCTGTGA GGAGGACCCG GACCTGAGCA CTCCCACAGA TGCTGTGGCT 
GACTCTGATG CCCGCCACTG GTTACAACTG AGTCCTACTG ATGCTTCAAA CTTAACAGAC 
AGCAGAGAAT GCCTTGCAGA CGACTGTAGC ATTATTGCTG GAGGAAACCT CACTGGTTGG 
CACCCAGACT CCGCTGCTGT GTTATGGCGA AGAGTCTTGG GAATCCTTGG AGATGTGAAT 
AACATCCAGT CGCCGAAGAT CCATGCCAAA GTTTTTGGCT ATCTCTATGA ACTGTGGTAT 
AAACTGGCCA AGATACGAGA TAATTTAGCA ATAAGCCTGG ATAACCAGTC GTCTCCATCT 
CCTCCTCTCT TGATCCCACC ACTCAGAATG TTTGCATCAT GGCTATTTAA GGCTACAACT 
CTGCCAAATG AATATAAAGA AGGCAAACTG CAAGCCTACA AACTGATCTG TGCCATGATG 
ACCAGACGCC AGGATGTTCT GCCAAACTCT GACTTCCTGG TGCATTTTTA CCTTGTGATG 
CACCTGGGAC TAACCAGCGA GGACCAGGAT GTCTTAAACA CCATCATAAA AAACTGCTCA 
CCCCGCTTTT TCTCCCTGGG TTTGCCTGGC TTCTCTATGC TGGTGGGGGA CTTTATCACT 
GCTGCTGCCA GGGTCCTCAG TACAGACATG TTGGCGGCAC CCCGTTCAGA AGCTCTCACT 
CTCCTTGGTT CCCTTGTCTG CTTCCCAAAT ACCTACCAGG AGATCCCTCT GCTGCAGTCA 
GTGCCAGAAG TGAGTGATGT TGTCACGGGA GCGGAAGACG TCAAGCATTA CCTCATAAAT 
ATTTTACTGA AGAATGCTAC CGAGGAACCA AATGAATGTG CAAGATGCAT CGCCATTTGC 
TCCCTCGGGG TCTGGATCTG TGAAGAGCTT GCACAGAGTG CCAGCCATCC TCAGGTGAAG 
GATGCCATCA ATGTGATAGG TGTAACTTTG AAGTTTCCTA ACAAGATCGT GGCTCAGGTA 
GCCTGTGATG TTCTTCAGTT GTTGGTTTCC TACTGGGAAA AGCTGCAGAT GTTTGAAACT 
GCTCTACCCC GCAAAATGGC AGAAATTCTT GTGGCCACAA TTGCGTTCCT TTTACCAAGT 
GCAGAGTACT CGTCAGTGGA AACAGATAAA AAGTTTATTG TGTCCTTGCT CCTTTGTCTC 
TTGGACTGGT GCATGGCGCT GCCCGTGAGT GCCCTCCTCC ACCCTGTGTC CACAGCAGTT 
CTAGAGGAGC TGCACCCATC CCGGGCCCCC TTGCTGGATT ATATCTACAG GGTTCTGCAC 
TGCTGTGTCT GTGGCTCCAG CACATACACA CAGCAAAGTC ACTATACTCT GACCTTGGCT 
GACCTGTCAT CCACGGATTA TGACCCCTTC CTACCTCTGG CAAACGTGAG GAACTCCGAG 
CCAATCCAGT ATCATTCATC AGCAGACCTG GGGAACCTGC TGACCGTGGA AGAGGAAAAG 
AAGAGAAGAA GTGTGGAATT GATCCCCCTC ACTGCACGAA TGGTCATGGC CCACCTAGTG 
AACCACCTAG GCCACTATCC ACTGAGTGGG GGTCCTGCTG TGCTGCACAG CCTGGTCAGT 
GAGAACCATG ACAATGCCCA TGTGGAAGGA ACTGAGCTGT CCTCGGAGGT GTTCAGGAGC 
CCAAACCTGC AGCTCTTCGT ATTTAATGAT AGCACCCTCA TCTCCTATCT TCAGACACCA 
GCAGAAGGAC CAGCTGGAGG GACTTCAGGG GGTTCCCTCT CAGATGTGAG AGTCATTGTG 
AGGGATATCT CAGGGAAGTA CTCTTGGGAT GGCAAGGTTT TATATGGACC TCTGGAAGGC 
CGTTTGGCAC CCAATGGAAG GAACCCCTCA TTTCAGATTT CTGGCTGGCA TCATCACACA 
TGTGGACCTC AGAAAGATTT GTTTAACGGT GAGGAGGGAG ATGATGTGCT TGACAAGTTA 
CTTGAAAACA TTGGCCATAC AAGCCCCGAA TGCCTTTTAC CATCACAACT AAATCTAAAT 
GAGCCTTCCC CAACCCCATG TGCAATGAAC TGGGACCAAG AGAAGGCAAT CATGGAGGTC 
ATCCTGCGCC AGAGCGCACA GGAAGATGAG TATGTGCAGA GGTGTAACTC GGATTCTTCA 
GTAACGGTCA CCAGCCAAGG GCAGCCCTCA CCCGTGGAGC CCCGGGGACC CTTTTATTTC 
TGCAGGTTGT TGCTTGATGA CTTGGGAATG AATTCCTGGG ACAGAAGGAA GAATTTTCAT 
TTGTTGAAGA AAAATTCAAA ATTATTGAGA GAGCTAAAGA ATCTGGACTC GCGCCAGTGC 
CGCGAGACCC ATAAGATTGC AGTGTTTTAC ATTGCTGAAG GGCAAGAAGA CAAGTGTTCC 
ATTCTAGCCA ATGAAAGAGG AAGCCAAGCA TATGAAGACT TTGTTGCTGG TCTTGGATGG 
GAGGTGGATC TCTCAACCCA CTGTGGGTTT ATGGGTGGTC TTCAGCGCAA TGGCAGCACG 
GGACAGACAG CCCCTTACTA TGCTACCTCG ACCGTGGAAG TAATTTTCCA CGTTTCCACT 
CGGATGCCAT CAGATTCAGA TGATTCTCTC ACCAAAAAGC TCCGTCACCT GGGGAATGAT 
GAGGTCCACA TCGTCTGGTC TGAACACTCC AGGGACTACC GCAGGGGTAT TATCCCAACA 
GCCTTTGGAG ACGTTTCCAT CATTATTTAC CCAATGAAGA ATCATATGTT CTTTATCACG 
ATAACGAAGA AGCCTGAGGT TCCATTCTTT GGGCCCCTGT TCGATGGAGC GATCGTGAGT 
GGGAAGCTGC TGCCAAGCCT TATCTGTGCC ACATGCATCA ATGCCAGCAG AGCGGTGAAG 
TGCCTCATCC CACTCTATCA GAGCTTCTAC GAAGAGCGAG CTCTGTATCT GGAAGCAATA 
ATTCAAAACC ACCGGGAAGT CATGACATTT GAGGATTTCG CTGCTCAAGT CTTTTCTCCC 
TCTCCCAGCT ACTCTGTCAG TGGAACGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028520.2
CDS	106..5838
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTD

Target ORF information:

RefSeq Version	NM_001033348.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033348.3

ORF Insert Sequence:

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGAAGTGGA GGAGTTCCAG 
CAGGCAGAGA GCACCGCCGC AGCCGACTGT GACTATCTTG TGGTAGGACA GCAGCAGGTG 
CCCCGGAGCA GCAGCACCTC TGACATCACT GAGCGCTTGT ACTCAGACTC CTCTCAAGGT 
TACAGCCCCA TGTTGCCTCA TCCTGCTTTT TCCACGACTT CTGGGGATCA GAGCTCTGGT 
CCTCATGCTT GCACAGCTGG CCCTTTACCT TTGAGCCGTC TCCCCAGTGC AGGTCAAAAG 
GTAGAACATT CGCAGAATTT AAGCTCTTCA GAACCCAAGT CTGTTCAAGA GAGTAAAGGA 
CATGTGACTC ATGAACATGA AGGAATAACA ATGTTGGTTC GAAGGAGCAG CAGTCCCGCT 
GAGCTGGAGC TGAAGGATGA TTTACAGCAG GCACATGGAA GATGCAGGCA GAGACAGACA 
AGTGAAAGTA CTGGTAGTGA CACAGTTGTG GGCTACAGCA ATGAAGCAGA GCTACCTGTG 
AGCCCGTGGC AGGCCTGTGA GGAGGACCCG GACCTGAGCA CTCCCACAGA TGCTGTGGCT 
GACTCTGATG CCCGCCACTG GTTACAACTG AGTCCTACTG ATGCTTCAAA CTTAACAGAC 
AGCAGAGAAT GCCTTGCAGA CGACTGTAGC ATTATTGCTG GAGGAAACCT CACTGGTTGG 
CACCCAGACT CCGCTGCTGT GTTATGGCGA AGAGTCTTGG GAATCCTTGG AGATGTGAAT 
AACATCCAGT CGCCGAAGAT CCATGCCAAA GTTTTTGGCT ATCTCTATGA ACTGTGGTAT 
AAACTGGCCA AGATACGAGA TAATTTAGCA ATAAGCCTGG ATAACCAGTC GTCTCCATCT 
CCTCCTCTCT TGATCCCACC ACTCAGAATG TTTGCATCAT GGCTATTTAA GGCTACAACT 
CTGCCAAATG AATATAAAGA AGGCAAACTG CAAGCCTACA AACTGATCTG TGCCATGATG 
ACCAGACGCC AGGATGTTCT GCCAAACTCT GACTTCCTGG TGCATTTTTA CCTTGTGATG 
CACCTGGGAC TAACCAGCGA GGACCAGGAT GTCTTAAACA CCATCATAAA AAACTGCTCA 
CCCCGCTTTT TCTCCCTGGG TTTGCCTGGC TTCTCTATGC TGGTGGGGGA CTTTATCACT 
GCTGCTGCCA GGGTCCTCAG TACAGACATG TTGGCGGCAC CCCGTTCAGA AGCTCTCACT 
CTCCTTGGTT CCCTTGTCTG CTTCCCAAAT ACCTACCAGG AGATCCCTCT GCTGCAGTCA 
GTGCCAGAAG TGAGTGATGT TGTCACGGGA GCGGAAGACG TCAAGCATTA CCTCATAAAT 
ATTTTACTGA AGAATGCTAC CGAGGAACCA AATGAATGTG CAAGATGCAT CGCCATTTGC 
TCCCTCGGGG TCTGGATCTG TGAAGAGCTT GCACAGAGTG CCAGCCATCC TCAGGTGAAG 
GATGCCATCA ATGTGATAGG TGTAACTTTG AAGTTTCCTA ACAAGATCGT GGCTCAGGTA 
GCCTGTGATG TTCTTCAGTT GTTGGTTTCC TACTGGGAAA AGCTGCAGAT GTTTGAAACT 
GCTCTACCCC GCAAAATGGC AGAAATTCTT GTGGCCACAA TTGCGTTCCT TTTACCAAGT 
GCAGAGTACT CGTCAGTGGA AACAGATAAA AAGTTTATTG TGTCCTTGCT CCTTTGTCTC 
TTGGACTGGT GCATGGCGCT GCCCGTGAGT GCCCTCCTCC ACCCTGTGTC CACAGCAGTT 
CTAGAGGAGC TGCACCCATC CCGGGCCCCC TTGCTGGATT ATATCTACAG GGTTCTGCAC 
TGCTGTGTCT GTGGCTCCAG CACATACACA CAGCAAAGTC ACTATACTCT GACCTTGGCT 
GACCTGTCAT CCACGGATTA TGACCCCTTC CTACCTCTGG CAAACGTGAG GAACTCCGAG 
CCAATCCAGT ATCATTCATC AGCAGACCTG GGGAACCTGC TGACCGTGGA AGAGGAAAAG 
AAGAGAAGAA GTGTGGAATT GATCCCCCTC ACTGCACGAA TGGTCATGGC CCACCTAGTG 
AACCACCTAG GCCACTATCC ACTGAGTGGG GGTCCTGCTG TGCTGCACAG CCTGGTCAGT 
GAGAACCATG ACAATGCCCA TGTGGAAGGA ACTGAGCTGT CCTCGGAGGT GTTCAGGAGC 
CCAAACCTGC AGCTCTTCGT ATTTAATGAT AGCACCCTCA TCTCCTATCT TCAGACACCA 
GCAGAAGGAC CAGCTGGAGG GACTTCAGGG GGTTCCCTCT CAGATGTGAG AGTCATTGTG 
AGGGATATCT CAGGGAAGTA CTCTTGGGAT GGCAAGGTTT TATATGGACC TCTGGAAGGC 
CGTTTGGCAC CCAATGGAAG GAACCCCTCA TTTCAGATTT CTGGCTGGCA TCATCACACA 
TGTGGACCTC AGAAAGATTT GTTTAACGGT GAGGAGGGAG ATGATGTGCT TGACAAGTTA 
CTTGAAAACA TTGGCCATAC AAGCCCCGAA TGCCTTTTAC CATCACAACT AAATCTAAAT 
GAGCCTTCCC CAACCCCATG TGCAATGAAC TGGGACCAAG AGAAGGCAAT CATGGAGGTC 
ATCCTGCGCC AGAGCGCACA GGAAGATGAG TATGTGCAGA GGTGTAACTC GGATTCTTCA 
GTAACGGTCA CCAGCCAAGG GCAGCCCTCA CCCGTGGAGC CCCGGGGACC CTTTTATTTC 
TGCAGGTTGT TGCTTGATGA CTTGGGAATG AATTCCTGGG ACAGAAGGAA GAATTTTCAT 
TTGTTGAAGA AAAATTCAAA ATTATTGAGA GAGCTAAAGA ATCTGGACTC GCGCCAGTGC 
CGCGAGACCC ATAAGATTGC AGTGTTTTAC ATTGCTGAAG GGCAAGAAGA CAAGTGTTCC 
ATTCTAGCCA ATGAAAGAGG AAGCCAAGCA TATGAAGACT TTGTTGCTGG TCTTGGATGG 
GAGGTGGATC TCTCAACCCA CTGTGGGTTT ATGGGTGGTC TTCAGCGCAA TGGCAGCACG 
GGACAGACAG CCCCTTACTA TGCTACCTCG ACCGTGGAAG TAATTTTCCA CGTTTCCACT 
CGGATGCCAT CAGATTCAGA TGATTCTCTC ACCAAAAAGC TCCGTCACCT GGGGAATGAT 
GAGGTCCACA TCGTCTGGTC TGAACACTCC AGGGACTACC GCAGGGGTAT TATCCCAACA 
GCCTTTGGAG ACGTTTCCAT CATTATTTAC CCAATGAAGA ATCATATGTT CTTTATCACG 
ATAACGAAGA AGCCTGAGGT TCCATTCTTT GGGCCCCTGT TCGATGGAGC GATCGTGAGT 
GGGAAGCTGC TGCCAAGCCT TATCTGTGCC ACATGCATCA ATGCCAGCAG AGCGGTGAAG 
TGCCTCATCC CACTCTATCA GAGCTTCTAC GAAGAGCGAG CTCTGTATCT GGAAGCAATA 
ATTCAAAACC ACCGGGAAGT CATGACATTT GAGGATTTCG CTGCTCAAGT CTTTTCTCCC 
TCTCCCAGCT ACTCTGTCAG TGGAACGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa2 ( XM_006499517.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499580.1 Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X2, mRNA.

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CloneID	OMu30472
Clone ID Related Accession (Same CDS sequence)	XM_006499517.3 , XM_006499517.4
Accession Version	XM_006499517.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ral GTPase-activating protein subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499517.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGT GAAAGAACAG 
GAGGAATCCA CAGCAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499580.1
CDS	326..6226
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATAKRSQSISNCVHLSEALPATKSVPLLLHTVSALLPGLSYSSCSHRLSEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTVKEQEESTAS

Target ORF information:

RefSeq Version	XM_006499517.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499517.3

ORF Insert Sequence:

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGT GAAAGAACAG 
GAGGAATCCA CAGCAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa2 ( XM_006499519.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499582.1 Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X3, mRNA.

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CloneID	OMu30473
Clone ID Related Accession (Same CDS sequence)	XM_006499519.4 , XM_006499519.3
Accession Version	XM_006499519.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ral GTPase-activating protein subunit alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499582.1
CDS	325..6198
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATAKRSQSISNCVHLSEALPATKSVPLLLHTVSALLPGLSYSSCSHRLSEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_006499519.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499519.3

ORF Insert Sequence:

ATGTTCTCCC GAAGGAGCCA CGGAGATGTG AAAAAGTCCA CGCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGATAA CGTGGATGCA 
AGCGATCTTA AGCAGTTCTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAG TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATCCTCTT TCTATTTGAA AAAATACTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTTT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC AGACTATTTC TTTTGTGGCT TCAAGCACTT 
CAGACAAACT GTGCAGAAGA GCAGGTGCTG ATTTTCGCTT GCCTAGTGCC TGGTTTCCCA 
GCGGTCTTGT CATCCAGGGG TCCCTGCACA CTGGAGACAC TCATCAACCC TAGCCCTAGT 
ATAGTTGATG CAAAAATATA CCCAGAGGAA ATCACCCCAC TTCTGCCAGC CATATCAGGG 
GAGAAGATTG CTGAGGACCA AACCTGCTTT TTTCTTCAAA TACTGTTGAA ATACATGGTT 
ATTCAGGCTG CAAGCTTGGA GTGGAAGAAT AAAGAGAATC AAGATACTGG TTTTAAATTT 
CTTTTTACGT TGTTTCGAAA GTATTATCTT CCTCATTTGT TTCCATCATT TACTAAATTA 
ACCAACATCT ACAAACCTGT ACTTGAAATT CCCCATTTGA GACCAAAGCC AGTATATGTT 
ACTGTAACTC GAGATAATGA AACAATTTAC AGTACAAAAA TTCCATACAT GGCAGCTCGT 
GTTGTTTTCA TTAAATGGAT TGTAACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAAAACACTA AAAATGGAGT TGATGTATTG CCTAAAATCA TCCAGACTGT TGGTGGTGGT 
GCAATACAAG AGAAGGTACC AGAGCTGGAT GGTGCTGGGT CCACAGAACA GGACAAAAGC 
CATTCTAACA GCAGCACCTT GTCTGACCGA AGACTCAGCA ACTCCAGTCT TTGTAGCATT 
GAAGAGGAGC ATCGGACAGT GTATGAGATG GTGCAGCGGA TCCTCCTGTC AACACGGGGT 
TATGTGAATT TTGTGAATGA AGTATTTCGA CAGGCATTTT TGTTGCCTTC CTGTGAGATA 
TCTGTAACAA GAAAAGTAGT TCAAGTGTAT AGGAAATGGA TCCTCCAGAA CAAACCTGTT 
TTCATGGAGG AACCAGATAA AAAGGATGTT GCTCAAGAAG ATGCTGACAA ATTAGGACTT 
TCAGAGACTG ACTCCAAGGA GGCCTCATCT GAAAGTTCTG GTCATAAACG GTCATCCAGT 
TGGGGACGCA CGTATTCCTT CACAAGTGCC ATGAGCAGAG GATGTGTGAC AGAGGAGGAC 
AACACAAATG TGAAAGCTGG GGCCCAAGCT ATGTTGCAGG TCTTTCTTAC GAATGCTGCA 
AATGTCTTTT TGCTGGAACC ATGTGCCGAA GTTCCCATGC TCCTGAGAGA ACAAGTAGAC 
GCCTCTAAAG CTGTTCTGAT TATTTTTCGG CGGATGATAA TGGAGCTTAC AATGAATCAA 
AAGACATGGG AGCAGATGTT GCAAATCCTT CTCAGGATAA CAGAAGCTGT CATGCAGAAG 
CCAAAGGATA AGCATGTCAA GGACTTGTTT GCCCAGAGCT TGGCAGGGTT GCTCTTCAGG 
ACACTTATTG TGGCTTGGAT CCGAGCAAAC CTCTGTGTGT ACATTTCTCG GGAGCTCTGG 
GATGACTTTC TCAGGGTGCT GTCATCCCTT ACGGAATGGG AGGAACTCAT CACTGAATGG 
TCTAACATCA TGGATTCCTT GACAGCCGTG CTTGCCAGAA CTGTGTATGG AGTTGAAATG 
ACAAACCTAC CTCTAGATAA GTTAAGTGAA CAAAAGGAGA AGAAGCAGCG TGGCAAAGGT 
TGTATCCTAG AACCCCAAAA AGGAACAGCT GTTGGGAGAT CCTTTTCTCT CAGCTGGCGT 
AGCCATCCAG ATGTGACTGA GCCAATGAGA TTCAGAAGTG CCACCACATC TGGAGCACCA 
GGAGTGGAGA AGGCAAGAAA TACTGTCCGT CAAAAAGCAA CTGCTAAGCG AAGCCAGTCT 
ATCAGCAACT GTGTCCACCT CTCTGAGGCT TTGCCTGCTA CTAAAAGCGT CCCGCTCCTC 
CTCCATACCG TGAGCGCTCT CTTACCTGGT CTCTCTTACT CCTCTTGCTC TCACAGGCTG 
TCAGAAGTGG AGGAGTTCCA GCAGGCAGAG AGCACCGCCG CAGCCGACTG TGACTATCTT 
GTGGTAGGAC AGCAGCAGGT GCCCCGGAGC AGCAGCACCT CTGACATCAC TGAGCGCTTG 
TACTCAGACT CCTCTCAAGG TTACAGCCCC ATGTTGCCTC ATCCTGCTTT TTCCACGACT 
TCTGGGGATC AGAGCTCTGG TCCTCATGCT TGCACAGCTG GCCCTTTACC TTTGAGCCGT 
CTCCCCAGTG CAGGTCAAAA GGTAGAACAT TCGCAGAATT TAAGCTCTTC AGAACCCAAG 
TCTGTTCAAG AGAGTAAAGG ACATGTGACT CATGAACATG AAGGAATAAC AATGTTGGTT 
CGAAGGAGCA GCAGTCCCGC TGAGCTGGAG CTGAAGGATG ATTTACAGCA GGCACATGGA 
AGATGCAGGC AGAGACAGAC AAGTGAAAGT ACTGGTAGTG ACACAGTTGT GGGCTACAGC 
AATGAAGCAG AGCTACCTGT GAGCCCGTGG CAGGCCTGTG AGGAGGACCC GGACCTGAGC 
ACTCCCACAG ATGCTGTGGC TGACTCTGAT GCCCGCCACT GGTTACAACT GAGTCCTACT 
GATGCTTCAA ACTTAACAGA CAGCAGAGAA TGCCTTGCAG ACGACTGTAG CATTATTGCT 
GGAGGAAACC TCACTGGTTG GCACCCAGAC TCCGCTGCTG TGTTATGGCG AAGAGTCTTG 
GGAATCCTTG GAGATGTGAA TAACATCCAG TCGCCGAAGA TCCATGCCAA AGTTTTTGGC 
TATCTCTATG AACTGTGGTA TAAACTGGCC AAGATACGAG ATAATTTAGC AATAAGCCTG 
GATAACCAGT CGTCTCCATC TCCTCCTCTC TTGATCCCAC CACTCAGAAT GTTTGCATCA 
TGGCTATTTA AGGCTACAAC TCTGCCAAAT GAATATAAAG AAGGCAAACT GCAAGCCTAC 
AAACTGATCT GTGCCATGAT GACCAGACGC CAGGATGTTC TGCCAAACTC TGACTTCCTG 
GTGCATTTTT ACCTTGTGAT GCACCTGGGA CTAACCAGCG AGGACCAGGA TGTCTTAAAC 
ACCATCATAA AAAACTGCTC ACCCCGCTTT TTCTCCCTGG GTTTGCCTGG CTTCTCTATG 
CTGGTGGGGG ACTTTATCAC TGCTGCTGCC AGGGTCCTCA GTACAGACAT GTTGGCGGCA 
CCCCGTTCAG AAGCTCTCAC TCTCCTTGGT TCCCTTGTCT GCTTCCCAAA TACCTACCAG 
GAGATCCCTC TGCTGCAGTC AGTGCCAGAA GTGAGTGATG TTGTCACGGG AGCGGAAGAC 
GTCAAGCATT ACCTCATAAA TATTTTACTG AAGAATGCTA CCGAGGAACC AAATGAATGT 
GCAAGATGCA TCGCCATTTG CTCCCTCGGG GTCTGGATCT GTGAAGAGCT TGCACAGAGT 
GCCAGCCATC CTCAGGTGAA GGATGCCATC AATGTGATAG GTGTAACTTT GAAGTTTCCT 
AACAAGATCG TGGCTCAGGT AGCCTGTGAT GTTCTTCAGT TGTTGGTTTC CTACTGGGAA 
AAGCTGCAGA TGTTTGAAAC TGCTCTACCC CGCAAAATGG CAGAAATTCT TGTGGCCACA 
ATTGCGTTCC TTTTACCAAG TGCAGAGTAC TCGTCAGTGG AAACAGATAA AAAGTTTATT 
GTGTCCTTGC TCCTTTGTCT CTTGGACTGG TGCATGGCGC TGCCCGTGAG TGCCCTCCTC 
CACCCTGTGT CCACAGCAGT TCTAGAGGAG CTGCACCCAT CCCGGGCCCC CTTGCTGGAT 
TATATCTACA GGGTTCTGCA CTGCTGTGTC TGTGGCTCCA GCACATACAC ACAGCAAAGT 
CACTATACTC TGACCTTGGC TGACCTGTCA TCCACGGATT ATGACCCCTT CCTACCTCTG 
GCAAACGTGA GGAACTCCGA GCCAATCCAG TATCATTCAT CAGCAGACCT GGGGAACCTG 
CTGACCGTGG AAGAGGAAAA GAAGAGAAGA AGTGTGGAAT TGATCCCCCT CACTGCACGA 
ATGGTCATGG CCCACCTAGT GAACCACCTA GGCCACTATC CACTGAGTGG GGGTCCTGCT 
GTGCTGCACA GCCTGGTCAG TGAGAACCAT GACAATGCCC ATGTGGAAGG AACTGAGCTG 
TCCTCGGAGG TGTTCAGGAG CCCAAACCTG CAGCTCTTCG TATTTAATGA TAGCACCCTC 
ATCTCCTATC TTCAGACACC AGCAGAAGGA CCAGCTGGAG GGACTTCAGG GGGTTCCCTC 
TCAGATGTGA GAGTCATTGT GAGGGATATC TCAGGGAAGT ACTCTTGGGA TGGCAAGGTT 
TTATATGGAC CTCTGGAAGG CCGTTTGGCA CCCAATGGAA GGAACCCCTC ATTTCAGATT 
TCTGGCTGGC ATCATCACAC ATGTGGACCT CAGAAAGATT TGTTTAACGG TGAGGAGGGA 
GATGATGTGC TTGACAAGTT ACTTGAAAAC ATTGGCCATA CAAGCCCCGA ATGCCTTTTA 
CCATCACAAC TAAATCTAAA TGAGCCTTCC CCAACCCCAT GTGCAATGAA CTGGGACCAA 
GAGAAGGCAA TCATGGAGGT CATCCTGCGC CAGAGCGCAC AGGAAGATGA GTATGTGCAG 
AGGTGTAACT CGGATTCTTC AGTAACGGTC ACCAGCCAAG GGCAGCCCTC ACCCGTGGAG 
CCCCGGGGAC CCTTTTATTT CTGCAGGTTG TTGCTTGATG ACTTGGGAAT GAATTCCTGG 
GACAGAAGGA AGAATTTTCA TTTGTTGAAG AAAAATTCAA AATTATTGAG AGAGCTAAAG 
AATCTGGACT CGCGCCAGTG CCGCGAGACC CATAAGATTG CAGTGTTTTA CATTGCTGAA 
GGGCAAGAAG ACAAGTGTTC CATTCTAGCC AATGAAAGAG GAAGCCAAGC ATATGAAGAC 
TTTGTTGCTG GTCTTGGATG GGAGGTGGAT CTCTCAACCC ACTGTGGGTT TATGGGTGGT 
CTTCAGCGCA ATGGCAGCAC GGGACAGACA GCCCCTTACT ATGCTACCTC GACCGTGGAA 
GTAATTTTCC ACGTTTCCAC TCGGATGCCA TCAGATTCAG ATGATTCTCT CACCAAAAAG 
CTCCGTCACC TGGGGAATGA TGAGGTCCAC ATCGTCTGGT CTGAACACTC CAGGGACTAC 
CGCAGGGGTA TTATCCCAAC AGCCTTTGGA GACGTTTCCA TCATTATTTA CCCAATGAAG 
AATCATATGT TCTTTATCAC GATAACGAAG AAGCCTGAGG TTCCATTCTT TGGGCCCCTG 
TTCGATGGAG CGATCGTGAG TGGGAAGCTG CTGCCAAGCC TTATCTGTGC CACATGCATC 
AATGCCAGCA GAGCGGTGAA GTGCCTCATC CCACTCTATC AGAGCTTCTA CGAAGAGCGA 
GCTCTGTATC TGGAAGCAAT AATTCAAAAC CACCGGGAAG TCATGACATT TGAGGATTTC 
GCTGCTCAAG TCTTTTCTCC CTCTCCCAGC TACTCTGTCA GTGGAACGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of Ral GTPase-activating protein promotes tumor invasion and metastasis of bladder cancer.
Oncogene32(7)894-902(2013 Feb)
Saito R,Shirakawa R,Nishiyama H,Kobayashi T,Kawato M,Kanno T,Nishizawa K,Matsui Y,Ohbayashi T,Horiguchi M,Nakamura T,Ikeda T,Yamane K,Nakayama E,Nakamura E,Toda Y,Kimura T,Kita T,Ogawa O,Horiuchi H

A Ral GAP complex links PI 3-kinase/Akt signaling to RalA activation in insulin action.
Molecular biology of the cell22(1)141-52(2011 Jan)
Chen XW,Leto D,Xiong T,Yu G,Cheng A,Decker S,Saltiel AR

Adipocytes contain a novel complex similar to the tuberous sclerosis complex.
Cellular signalling18(10)1626-32(2006 Oct)
Gridley S,Chavez JA,Lane WS,Lienhard GE

Novel insulin-elicited phosphoproteins in adipocytes.
Cellular signalling17(1)59-66(2005 Jan)
Gridley S,Lane WS,Garner CW,Lienhard GE

Transcriptome analysis of mouse stem cells and early embryos.
PLoS biology1(3)E74(2003 Dec)
Sharov AA,Piao Y,Matoba R,Dudekula DB,Qian Y,VanBuren V,Falco G,Martin PR,Stagg CA,Bassey UC,Wang Y,Carter MG,Hamatani T,Aiba K,Akutsu H,Sharova L,Tanaka TS,Kimber WL,Yoshikawa T,Jaradat SA,Pantano S,Nagaraja R,Boheler KR,Taub D,Hodes RJ,Longo DL,Schlessinger D,Keller J,Klotz E,Kelsoe G,Umezawa A,Vescovi AL,Rossant J,Kunath T,Hogan BL,Curci A,D'Urso M,Kelso J,Hide W,Ko MS

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Ralgapa2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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