Spon1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spon1
Entrez Gene ID	233744
Full Name	spondin 1, (f-spondin) extracellular matrix protein
Synonyms	AI666765,AW455831,BC020531,D330035F22Rik,FSP
General protein information	Preferred Names spondin 1, (f-spondin) extracellular matrix protein Names spondin-1 F-spondin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_145584.2	NP_663559.1	spondin-1 precursor

Pathways from BioSystems

Homologies

	SPON1	XP_001093336.1
Mus musculus (house mouse)	Spon1	NP_663559.1
Xenopus tropicalis (tropical clawed frog)	spon1	NP_001090741.1
Homo sapiens (human)	SPON1	NP_006099.2
Rattus norvegicus (Norway rat)	Spon1	NP_742064.1
Gallus gallus (chicken)	SPON1	NP_990182.1
Danio rerio (zebrafish)	spon1a	NP_571590.1
Drosophila melanogaster (fruit fly)	CG17739	NP_610757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012307	XP_320234.3
Pan troglodytes (chimpanzee)	SPON1	XP_508295.3
Canis lupus familiaris (dog)	SPON1	XP_534067.2
Bos taurus (cattle)	SPON1	NP_777168.1

Gene Ontology
Bibliography

Related articles in PubMed

TLR4 induces CREB-mediated IL-6 production via upregulation of F-spondin to promote vascular smooth muscle cell migration.
Lee GL, Wu JY, Yeh CC, Kuo CC
Biochemical and biophysical research communications473(4)1205-1210(2016 05)

Excessive Wnt/beta-catenin signaling promotes midbrain floor plate neurogenesis, but results in vacillating dopamine progenitors.
Nouri N, Patel MJ, Joksimovic M, Poulin JF, Anderegg A, Taketo MM, Ma YC, Awatramani R
Molecular and cellular neurosciences68131-42(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis.
Title: TLR4 induces CREB-mediated IL-6 production via upregulation of F-spondin to promote vascular smooth muscle cell migration.

These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages.
Title: Stimulation of Proliferation and Migration of Mouse Macrophages by Type B CpG-ODNs Is F-Spondin and IL-1Ra Dependent.

Spon1 deletion leads to a local and systemic reduction of TGF-beta levels resulting in increased BMP signaling and increased bone deposition in adult mice.
Title: F-spondin deficient mice have a high bone mass phenotype.

F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization.
Title: F-spondin regulates chondrocyte terminal differentiation and endochondral bone formation.

The following Spon1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spon1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11827	NM_145584.2 Latest version!	Mus musculus spondin 1, (f-spondin) extracellular matrix protein (Spon1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spon1 ( NM_145584.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663559.1 Mus musculus spondin 1, (f-spondin) extracellular matrix protein (Spon1), mRNA.

Price & Availability↑

CloneID	OMu11827
Clone ID Related Accession (Same CDS sequence)	NM_145584.2
Accession Version	NM_145584.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	spondin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC103664.12, AC158213.4, AC118201.8 and AC102861.12. On Jul 30, 2009 this sequence version replaced NM_145584.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC020531.1, AK146506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCTGT CTCCCGTGTC CCTGAGGCTT AGCCGGGGTC CGGCGCTGCT GGCCCTCGCG 
CTGCCCCTGG CCGCAGCGCT AGCTTTCTCA GATGAGACCC TGGACAAAGT GACCAAGTCG 
GAGGGCTACT GCAGCCGCAT CTTGCGCGCC CAGGGCACAC GGCGTGAGGG ATACACGGAG 
TTCAGCCTCC GCGTGGAAGG CGACCCTGAC TTCTATAAGC CAGGAAGCAG CTACCGAGTG 
ACACTTTCGG CTGCTCCTCC CTCCTACTTC AGAGGTTTCA CGTTAATTGC CCTCAAAGAG 
AACCAGGAGG GAGATAAGGA AGAAGATCAT GCAGGCACCT TCCAGATCAT AGATGAGGAA 
GAGACCCAGT TTATGAGTAA CTGTCCTGTG GCCGTCACTG AAAGCACCCC TCGGAGGAGG 
ACACGGATCC AGGTGTTTTG GATAGCGCCA CCAACAGGGA CAGGCTGTGT GATTCTGAAG 
GCCAGCATTG TACAGAAACG CATAATTTAT TTTCAAGACG AGGGCTCCCT GACCAAGAAG 
CTGTGTGAAC AGGATCCCAC ACTTGATGGG GTGACGGACA GACCCATCTT AGACTGCTGC 
GCCTGTGGAA CTGCCAAGTA CAGACTCACG TTTTATGGGA ACTGGTCCGA GAAGACTCAT 
CCGAAGGATT ACCCTCGTCG GGCTAACCAC TGGTCTGCCA TCATTGGTGG GTCCCACTCC 
AAGAACTACG TGCTGTGGGA GTATGGAGGG TATGCCAGCG AAGGGGTCAA GCAAGTTGCT 
GAACTGGGCT CACCAGTAAA AATGGAGGAA GAAATTCGAC AACAGAGTGA TGAAGTCCTC 
ACTGTCATCA AAGCCAAAGC CCAGTGGCCA GCCTGGCAGC CTGTCAATGT GAGAGCAGCA 
CCCTCAGCTG AATTCTCAGT GGACAGGACA CGCCACTTGA TGTCCTTCCT CACCATGATG 
GGCCCCAGTC CAGACTGGAA CGTGGGCCTA TCTGCAGAGG ATCTGTGCAC CAAGGAGTGT 
GGCTGGGTCC AGAAAGTAGT GCAGGACCTG ATTCCCTGGG ATGCTGGCAC TGACAGCGGC 
GTGACCTATG AGTCACCAAA CAAGCCCACA ATTCCTCAGG AAAAAATCCG ACCCCTGACT 
AGTCTGGATC ATCCTCAGAG TCCTTTCTAT GACCCAGAAG GTGGGTCCAT CACACAAGTG 
GCCAGAGTCG TCATCGAGAG AATTGCCCGG AAGGGAGAAC AGTGCAACAT TGTACCTGAC 
AACGTGGACG ATATTGTAGC CGACCTGGCT CCAGAAGAGA AAGATGAAGA TGACACCCCT 
GAAACCTGCA TCTACTCCAA CTGGTCCCCA TGGTCGGCCT GCAGCTCTTC CACTTGTGAA 
AAGGGCAAGA GGATGCGGCA GCGCATGCTG AAGGCACAGC TGGACCTCAG TGTCCCCTGT 
CCTGACACCC AGGACTTCCA GCCCTGCATG GGCCCAGGCT GCAGCGATGA AGATGGCTCC 
ACCTGTACCA TGTCGGAGTG GATCACCTGG TCGCCCTGTA GCGTCTCGTG CGGCATGGGC 
ATGAGGTCCC GGGAGAGATA CGTGAAGCAG TTCCCGGAAG ACGGCTCAGT GTGCATGCTG 
CCCACGGAGG AGACGGAGAA GTGCACAGTC AACGAGGAGT GCTCTCCTAG CAGCTGCCTG 
GTGACTGAGT GGGGTGAGTG GGACGACTGC AGTGCCACCT GTGGGATGGG TATGAAGAAG 
AGGCACCGTA TGGTCAAGAT GAGCCCCGCG GACGGCTCCA TGTGCAAGGC AGAGACATCG 
CAGGCGGAGA AATGCATGAT GCCTGAGTGC CATACCATCC CGTGCTTGCT GTCTCCCTGG 
TCCGAGTGGA GCGACTGTAG CGTGACCTGT GGGAAGGGCA TGCGGACCCG CCAGCGGATG 
CTCAAATCTC TGGCAGAGCT GGGTGACTGT AACGAGGACC TGGAGCAGGC GGAGAAGTGC 
ATGCTGCCAG AATGCCCCAT TGACTGTGAA CTCAGCGAGT GGTCCCAGTG GTCTGAATGT 
AACAAGTCAT GTGGGAAAGG TCACATGATT CGAACCCGGA CAATCCAAAT GGAACCTCAG 
TTTGGGGGTG TACCCTGCCC AGAGACTGTG CAACGCAAGA AGTGCCGCAC CCGGAAATGC 
CTTCGCAGCC CATCAGTCCA GAAGCTGCGC TGGAGGGAGG CCCGAGAGAG CAGGAGGAGT 
GAGCAGCTGA GAGAAGAGTC AGATGGAGAG CAGTTCCCAG GCTGTCGAAT GCGCCCGTGG 
ACAGCCTGGT CAGAATGCAC CAAACTGTGC GGAGGTGGGA TCCAAGAGCG CTACATGACT 
GTGAAGAAGA GGTTCAAAAG CTCCCAGTTT ACCAGCTGCA AAGACAAGAA GGAGATCAGA 
GCGTGCAACG TGCACCCTTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663559.1
CDS	332..2755
Translation	MRLSPVSLRLSRGPALLALALPLAAALAFSDETLDKVTKSEGYCSRILRAQGTRREGYTEFSLRVEGDPDFYKPGSSYRVTLSAAPPSYFRGFTLIALKENQEGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDPTLDGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGMGMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSCGKGHMIRTRTIQMEPQFGGVPCPETVQRKKCRTRKCLRSPSVQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTVKKRFKSSQFTSCKDKKEIRACNVHPC

Target ORF information:

RefSeq Version	NM_145584.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spondin 1, (f-spondin) extracellular matrix protein (Spon1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145584.2

ORF Insert Sequence:

ATGAGGCTGT CTCCCGTGTC CCTGAGGCTT AGCCGGGGTC CGGCGCTGCT GGCCCTCGCG 
CTGCCCCTGG CCGCAGCGCT AGCTTTCTCA GATGAGACCC TGGACAAAGT GACCAAGTCG 
GAGGGCTACT GCAGCCGCAT CTTGCGCGCC CAGGGCACAC GGCGTGAGGG ATACACGGAG 
TTCAGCCTCC GCGTGGAAGG CGACCCTGAC TTCTATAAGC CAGGAAGCAG CTACCGAGTG 
ACACTTTCGG CTGCTCCTCC CTCCTACTTC AGAGGTTTCA CGTTAATTGC CCTCAAAGAG 
AACCAGGAGG GAGATAAGGA AGAAGATCAT GCAGGCACCT TCCAGATCAT AGATGAGGAA 
GAGACCCAGT TTATGAGTAA CTGTCCTGTG GCCGTCACTG AAAGCACCCC TCGGAGGAGG 
ACACGGATCC AGGTGTTTTG GATAGCGCCA CCAACAGGGA CAGGCTGTGT GATTCTGAAG 
GCCAGCATTG TACAGAAACG CATAATTTAT TTTCAAGACG AGGGCTCCCT GACCAAGAAG 
CTGTGTGAAC AGGATCCCAC ACTTGATGGG GTGACGGACA GACCCATCTT AGACTGCTGC 
GCCTGTGGAA CTGCCAAGTA CAGACTCACG TTTTATGGGA ACTGGTCCGA GAAGACTCAT 
CCGAAGGATT ACCCTCGTCG GGCTAACCAC TGGTCTGCCA TCATTGGTGG GTCCCACTCC 
AAGAACTACG TGCTGTGGGA GTATGGAGGG TATGCCAGCG AAGGGGTCAA GCAAGTTGCT 
GAACTGGGCT CACCAGTAAA AATGGAGGAA GAAATTCGAC AACAGAGTGA TGAAGTCCTC 
ACTGTCATCA AAGCCAAAGC CCAGTGGCCA GCCTGGCAGC CTGTCAATGT GAGAGCAGCA 
CCCTCAGCTG AATTCTCAGT GGACAGGACA CGCCACTTGA TGTCCTTCCT CACCATGATG 
GGCCCCAGTC CAGACTGGAA CGTGGGCCTA TCTGCAGAGG ATCTGTGCAC CAAGGAGTGT 
GGCTGGGTCC AGAAAGTAGT GCAGGACCTG ATTCCCTGGG ATGCTGGCAC TGACAGCGGC 
GTGACCTATG AGTCACCAAA CAAGCCCACA ATTCCTCAGG AAAAAATCCG ACCCCTGACT 
AGTCTGGATC ATCCTCAGAG TCCTTTCTAT GACCCAGAAG GTGGGTCCAT CACACAAGTG 
GCCAGAGTCG TCATCGAGAG AATTGCCCGG AAGGGAGAAC AGTGCAACAT TGTACCTGAC 
AACGTGGACG ATATTGTAGC CGACCTGGCT CCAGAAGAGA AAGATGAAGA TGACACCCCT 
GAAACCTGCA TCTACTCCAA CTGGTCCCCA TGGTCGGCCT GCAGCTCTTC CACTTGTGAA 
AAGGGCAAGA GGATGCGGCA GCGCATGCTG AAGGCACAGC TGGACCTCAG TGTCCCCTGT 
CCTGACACCC AGGACTTCCA GCCCTGCATG GGCCCAGGCT GCAGCGATGA AGATGGCTCC 
ACCTGTACCA TGTCGGAGTG GATCACCTGG TCGCCCTGTA GCGTCTCGTG CGGCATGGGC 
ATGAGGTCCC GGGAGAGATA CGTGAAGCAG TTCCCGGAAG ACGGCTCAGT GTGCATGCTG 
CCCACGGAGG AGACGGAGAA GTGCACAGTC AACGAGGAGT GCTCTCCTAG CAGCTGCCTG 
GTGACTGAGT GGGGTGAGTG GGACGACTGC AGTGCCACCT GTGGGATGGG TATGAAGAAG 
AGGCACCGTA TGGTCAAGAT GAGCCCCGCG GACGGCTCCA TGTGCAAGGC AGAGACATCG 
CAGGCGGAGA AATGCATGAT GCCTGAGTGC CATACCATCC CGTGCTTGCT GTCTCCCTGG 
TCCGAGTGGA GCGACTGTAG CGTGACCTGT GGGAAGGGCA TGCGGACCCG CCAGCGGATG 
CTCAAATCTC TGGCAGAGCT GGGTGACTGT AACGAGGACC TGGAGCAGGC GGAGAAGTGC 
ATGCTGCCAG AATGCCCCAT TGACTGTGAA CTCAGCGAGT GGTCCCAGTG GTCTGAATGT 
AACAAGTCAT GTGGGAAAGG TCACATGATT CGAACCCGGA CAATCCAAAT GGAACCTCAG 
TTTGGGGGTG TACCCTGCCC AGAGACTGTG CAACGCAAGA AGTGCCGCAC CCGGAAATGC 
CTTCGCAGCC CATCAGTCCA GAAGCTGCGC TGGAGGGAGG CCCGAGAGAG CAGGAGGAGT 
GAGCAGCTGA GAGAAGAGTC AGATGGAGAG CAGTTCCCAG GCTGTCGAAT GCGCCCGTGG 
ACAGCCTGGT CAGAATGCAC CAAACTGTGC GGAGGTGGGA TCCAAGAGCG CTACATGACT 
GTGAAGAAGA GGTTCAAAAG CTCCCAGTTT ACCAGCTGCA AAGACAAGAA GGAGATCAGA 
GCGTGCAACG TGCACCCTTG TTAA