Slc6a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc6a1
Entrez Gene ID	232333
Full Name	solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms	A730043E01,GABATHG,GABATR,GAT-1,Gabt,Gabt1,Gat1,XT-1,Xtrp1
General protein information	Preferred Names solute carrier family 6 (neurotransmitter transporter, GABA), member 1 Names sodium- and chloride-dependent GABA transporter 1 gamma-aminobutyric acid (GABA-A) transporter 1 solute carrier family 6 member 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_178703.4	NP_848818.1	sodium- and chloride-dependent GABA transporter 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC6A1	NP_003033.3
Canis lupus familiaris (dog)	SLC6A1	XP_541770.2
	SLC6A1	XP_001088606.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009768	XP_318854.4
Gallus gallus (chicken)	SLC6A1	XP_414303.1
Pan troglodytes (chimpanzee)	SLC6A1	XP_516280.3
Mus musculus (house mouse)	Slc6a1	NP_848818.1
Danio rerio (zebrafish)	slc6a1a	NP_001038752.2
Drosophila melanogaster (fruit fly)	CG1732	NP_651930.2
Caenorhabditis elegans (roundworm)	snf-11	NP_505873.2
Bos taurus (cattle)	SLC6A1	NP_001071304.1
Rattus norvegicus (Norway rat)	Slc6a1	NP_077347.1
Danio rerio (zebrafish)	slc6a1b	NP_001007363.1
Xenopus tropicalis (tropical clawed frog)	LOC100127682	NP_001106493.1

Gene Ontology
Bibliography

Related articles in PubMed

Defects During Mecp2 Null Embryonic Cortex Development Precede the Onset of Overt Neurological Symptoms.
Bedogni F, Cobolli Gigli C, Pozzi D, Rossi RL, Scaramuzza L, Rossetti G, Pagani M, Kilstrup-Nielsen C, Matteoli M, Landsberger N
Cerebral cortex (New York, N.Y. : 1991)26(6)2517-2529(2016 06)

Hyperactivity and impaired attention in Gamma aminobutyric acid transporter subtype 1 gene knockout mice.
Chen L, Yang X, Zhou X, Wang C, Gong X, Chen B, Chen Y
Acta neuropsychiatrica27(6)368-74(2015 Dec)

Exocytosis regulates trafficking of GABA and glycine heterotransporters in spinal cord glutamatergic synapses: a mechanism for the excessive heterotransporter-induced release of glutamate in experimental amyotrophic lateral sclerosis.
Milanese M, Bonifacino T, Fedele E, Rebosio C, Cattaneo L, Benfenati F, Usai C, Bonanno G
Neurobiology of disease74314-24(2015 Feb)

Comprehensive analysis of the GABAergic system gene expression profile in the anterior cingulate cortex of mice with Paclitaxel-induced neuropathic pain.
Masocha W
Gene expression16(3)145-53(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrated that GAT-1-/- mice have phenotypes of hyperactivity, impaired sustained attention and learning deficiency, and the performance of GAT-1-/- mice is similar to ADHD symptoms.
Title: Hyperactivity and impaired attention in Gamma aminobutyric acid transporter subtype 1 gene knockout mice.

Results suggest that the excessive GAT-1 and GlyT-1/2 heterotransporter-mediated Glu release, in the spinal cord of SOD1(G93A) mice, is due to the heterotransporter over-expression at the nerve terminal membrane, promoted by the excessive Glu exocytosis
Title: Exocytosis regulates trafficking of GABA and glycine heterotransporters in spinal cord glutamatergic synapses: a mechanism for the excessive heterotransporter-induced release of glutamate in experimental amyotrophic lateral sclerosis.

these data show that during paclitaxel-induced neuropathic pain there is significant increase in GAT-1 expression in the ACC. GAT-1 is the main transporter of GABA from the synapse
Title: Comprehensive analysis of the GABAergic system gene expression profile in the anterior cingulate cortex of mice with Paclitaxel-induced neuropathic pain.

Results demonstrate that high-affinity activation by GABA of GAT1 transporters provokes release of [3H]GABA from nerve terminals by different mechanisms
Title: High-affinity GABA uptake by neuronal GAT1 transporters provokes release of [(3)H]GABA by homoexchange and through GAT1-independent Ca(2+)-mediated mechanisms.

Development of GABA-removal system and its relationship to development of GABAergic networks studied by examination of changes in GAT-1 and GAT-3 expression and localization in embryonic and postnatal mouse spinal cord
Title: Characteristic development of the GABA-removal system in the mouse spinal cord.

The following Slc6a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc6a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18106	NM_178703.4 Latest version!	Mus musculus solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (Slc6a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc6a1 ( NM_178703.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848818.1 Mus musculus solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (Slc6a1), mRNA.

Price & Availability↑

CloneID	OMu18106
Clone ID Related Accession (Same CDS sequence)	NM_178703.4
Accession Version	NM_178703.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	sodium- and chloride-dependent GABA transporter 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB519886.1, AK053883.1 and BC059080.1. On Jul 11, 2009 this sequence version replaced NM_178703.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC059080.1, AK042956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACTGAGGT CAGCGAGGCC 
CCTGTGGCCA GCGACAAGCC CAAAACCCTG GTAGTCAAGG TGCAGAAGAA GGCCGGGGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG CGTGGGCTAT 
GCCATCGGCC TGGGCAATGT GTGGAGGTTC CCTTACCTCT GTGGGAAAAA CGGTGGCGGG 
GCCTTCCTAA TCCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCGCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATCTACTAC 
ATCGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACCCTGCCA 
TGGAAACAGT GTGACAACCC GTGGAACACT GACCGCTGCT TCTCCAACTA CAGCCTGGTC 
AATACCACCA ACATGACCAG CGCCGTGGTG GAGTTCTGGG AGCGCAACAT GCACCAGATG 
ACAGATGGAC TGGACAAGCC AGGACAGATC CGCTGGCCTC TGGCCATCAC ACTGGCCATT 
GCCTGGGTGC TCGTGTATTT CTGCATCTGG AAGGGTGTTG GTTGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACGTACCC CTACATCATG CTTATCATCC TGTTCTTCCG TGGAGTGACG 
CTTCCCGGGG CCAAGGAGGG GATCCTCTTC TACATCACAC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG GCTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATCGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGATTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CTGATGTGGC AGCCTCAGGC 
CCGGGGCTGG CATTCTTGGC GTACCCTGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAC GAGTACCCCA GACTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTGTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTT GATTATTACT CTGCCAGCGG CATGAGCTTG 
CTGTTCCTGG TTTTCTTCGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA TGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTGAAG 
GGCTCCCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT TGTGCGCCCT 
GAGAATGGCC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848818.1
CDS	380..2179
Translation	MATDNSKVADGQISTEVSEAPVASDKPKTLVVKVQKKAGDLPDRDTWKGRFDFLMSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLECSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIISWAIYYLYNSFTTTLPWKQCDNPWNTDRCFSNYSLVNTTNMTSAVVEFWERNMHQMTDGLDKPGQIRWPLAITLAIAWVLVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNFRKLSDSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFAGFVIFSIVGFMAHVTKRSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEYPRLLRNRRELFIAAVCIVSYLIGLSNITQGGIYVFKLFDYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGSRPCIWWKLCWSFFTPIIVAGVFLFSAVQMTPLTMGSYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLKGSLKQRLQVMIQPSEDIVRPENGPEQPQAGSSASKEAYI

Target ORF information:

RefSeq Version	NM_178703.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (Slc6a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178703.4

ORF Insert Sequence:

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACTGAGGT CAGCGAGGCC 
CCTGTGGCCA GCGACAAGCC CAAAACCCTG GTAGTCAAGG TGCAGAAGAA GGCCGGGGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG CGTGGGCTAT 
GCCATCGGCC TGGGCAATGT GTGGAGGTTC CCTTACCTCT GTGGGAAAAA CGGTGGCGGG 
GCCTTCCTAA TCCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCGCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATCTACTAC 
ATCGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACCCTGCCA 
TGGAAACAGT GTGACAACCC GTGGAACACT GACCGCTGCT TCTCCAACTA CAGCCTGGTC 
AATACCACCA ACATGACCAG CGCCGTGGTG GAGTTCTGGG AGCGCAACAT GCACCAGATG 
ACAGATGGAC TGGACAAGCC AGGACAGATC CGCTGGCCTC TGGCCATCAC ACTGGCCATT 
GCCTGGGTGC TCGTGTATTT CTGCATCTGG AAGGGTGTTG GTTGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACGTACCC CTACATCATG CTTATCATCC TGTTCTTCCG TGGAGTGACG 
CTTCCCGGGG CCAAGGAGGG GATCCTCTTC TACATCACAC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG GCTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATCGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGATTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CTGATGTGGC AGCCTCAGGC 
CCGGGGCTGG CATTCTTGGC GTACCCTGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAC GAGTACCCCA GACTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTGTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTT GATTATTACT CTGCCAGCGG CATGAGCTTG 
CTGTTCCTGG TTTTCTTCGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA TGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTGAAG 
GGCTCCCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT TGTGCGCCCT 
GAGAATGGCC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defects During Mecp2 Null Embryonic Cortex Development Precede the Onset of Overt Neurological Symptoms.
Cerebral cortex (New York, N.Y. : 1991)26(6)2517-2529(2016 06)
Bedogni F,Cobolli Gigli C,Pozzi D,Rossi RL,Scaramuzza L,Rossetti G,Pagani M,Kilstrup-Nielsen C,Matteoli M,Landsberger N

Hyperactivity and impaired attention in Gamma aminobutyric acid transporter subtype 1 gene knockout mice.
Acta neuropsychiatrica27(6)368-74(2015 Dec)
Chen L,Yang X,Zhou X,Wang C,Gong X,Chen B,Chen Y

Exocytosis regulates trafficking of GABA and glycine heterotransporters in spinal cord glutamatergic synapses: a mechanism for the excessive heterotransporter-induced release of glutamate in experimental amyotrophic lateral sclerosis.
Neurobiology of disease74314-24(2015 Feb)
Milanese M,Bonifacino T,Fedele E,Rebosio C,Cattaneo L,Benfenati F,Usai C,Bonanno G

Comprehensive analysis of the GABAergic system gene expression profile in the anterior cingulate cortex of mice with Paclitaxel-induced neuropathic pain.
Gene expression16(3)145-53(2015)
Masocha W