Lct cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lct
Entrez Gene ID	226413
Full Name	lactase
Synonyms	Gm100,LAC,LPH,Lphl
General protein information	Preferred Names lactase Names lactase-phlorizin hydrolase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E3\|1 55.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081078.2	NP_001074547.1	lactase-phlorizin hydrolase preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LCT	XP_003954409.1
	LCT	XP_001096426.2
Canis lupus familiaris (dog)	LCT	XP_005632060.1
Mus musculus (house mouse)	Lct	NP_001074547.1
Danio rerio (zebrafish)	lct	XP_001336765.5
Xenopus tropicalis (tropical clawed frog)	LOC101732850	XP_004917683.1
Rattus norvegicus (Norway rat)	Lct	NP_446293.1
Gallus gallus (chicken)	LCT	NP_001104816.1
Xenopus tropicalis (tropical clawed frog)	lct.2	XP_002934014.2
Homo sapiens (human)	LCT	NP_002290.2
Bos taurus (cattle)	LCT	NP_001192716.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Transcriptional heterogeneity in the lactase gene within cell-type is linked to the epigenome.
Oh E, Jeremian R, Oh G, Groot D, Susic M, Lee K, Foy K, Laird PW, Petronis A, Labrie V
Scientific reports741843(2017 Jan)

Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging.
Labrie V, Buske OJ, Oh E, Jeremian R, Ptak C, Gasi?nas G, Maleckas A, Petereit R, ?virbliene A, Adamonis K, Kriukien? E, Koncevi?ius K, Gordevi?ius J, Nair A, Zhang A, Ebrahimi S, Oh G, ?ik?nys V, Kup?inskas L, Brudno M, Petronis A
Nature structural & molecular biology23(6)566-73(2016 06)

GATA4 and GATA6 regulate intestinal epithelial cytodifferentiation during development.
Walker EM, Thompson CA, Battle MA
Developmental biology392(2)283-94(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic factors contribute to epigenetic changes occurring with age at the regulatory elements, because lactase-persistence and lactase-nonpersistence DNA haplotypes demonstrated markedly different epigenetic aging.
Title: Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging.

Data found transport defects of the intestinal brush border hydrolases lactase-phlorizin hydrolase (LPH) in galectin-3-null mutant mice, showing that it directly interact with galectin-3 and transit through non-raft-dependent apical transport platforms.
Title: Loss of galectin-3 impairs membrane polarisation of mouse enterocytes in vivo.

Inactivation of Gata4 had no effect on LPH gene expression, whereas inactivation of Hnf1alpha resulted in a 50% reduction in LPH gene expression during either cytodifferentiation or suckling.
Title: Gata4 and Hnf1alpha are partially required for the expression of specific intestinal genes during development.

Lactase gene promoter fragments mediate differential spatial and temporal expression patterns in transgenic mice.
Title: Lactase gene promoter fragments mediate differential spatial and temporal expression patterns in transgenic mice.

The following Lct gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lct cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01662	NM_001081078.2 Latest version!	Mus musculus lactase (Lct), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1420.30 $2029.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lct ( NM_001081078.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074547.1 Mus musculus lactase (Lct), mRNA.

Price & Availability↑

CloneID	OMu01662
Clone ID Related Accession (Same CDS sequence)	NM_001081078.2
Accession Version	NM_001081078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	lactase-phlorizin hydrolase preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161809.3. On Sep 1, 2015 this sequence version replaced NM_001081078.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGC CTTGGACAGC ACTCTTCCTC AGTACCTTCC TCCTGGGTCT TTCATGCCAG 
GGGAATGACT GGGAATCTGA CAGAAATTTT ATTTCAGCTG CTGGTCCTCT AACAAATGAC 
TTAGTGCACA ATCTGAACCA TCCGCTGGGA AAGCAGGGCT CTGATTTGGT AGCTGGTGAT 
ACTGACATCT ATGTCTGCCA GCAGCCACTG CCCTCCTTCT TGCCTCAGTA CTTCAGCAGT 
CTTCGGGCCA GCCAGGTTAC CCATTACAAA GTACTTCTGT CATGGGCACA ACTTCTCCCA 
AAAGGAAGCT CCAAGAATCC AGACCAGGAA GCAGTGCAGT GCTACCGACA ACTCCTCCAG 
TCCCTGAAGG ATGCCCAGCT TCAGCCCATG GTGGTCCTGT TCCACCAGAT GCCACCTACC 
AGCACCATCC AGAGAGACGG GGGCTTTGCT GACCTCTTTG CTGATTATGC CACATTGGCT 
TTTCAGTCCT TTGGGGACCT TGTTGAGATC TGGTTCACCT TCAGTGACTT GGAGAAAGTG 
ATCATGGGTC TTCCCCACCA GCATTTGAAA GCTTCTGGTC TCCAAACCCT CAGCGATGCC 
CACAGGAAAG CCTTTGACGT TTACCACAGA AAGTACTCTT CTCAGGGAGG AAAGCTGTCT 
GTGGTCCTGA AGGCTGAAGA CCTCCCCAAG CTTCTGCCAG ACCCATCCTC AGCGGCTCTC 
GTCAAGGGCT CCGTGGATTT CCTCTCTCTT GATTTGTCTT ATGACTGCCA AAGTATATCG 
ACTCTACCAC AGAAGCTGAG TGAATTGCAG AACCTTGAGC CCAAAGTGAA AGTTTTCGTC 
TACACCCTAA AACCTCAGGA CTGCCCATCG ACTGGAATGA GCCCCGCCTC ACTGCTCTTC 
AGACTCCTGG AAGCCATAAA TAAAGACCAA GTCCAGACTG TTGGGTTTGA TGTCAGTGCC 
TTCCTGAGCT GTACGTCCAG TTCTGAGGAG AGCCCATCTT GTTCTTTAAC CTACAGTAGC 
CTGGCCCTTC AGAGCGAGCA GCAGCATGAG ACAGTTGTGT CCTCATTCCC TGGGTCGGCC 
TACCAGAGAG TCTGGGCAGC CTTGGCTAAC CAGTCTAGGG AGGAAAGGGA CGCTTTCCTG 
CAAGATGTAT TTCCAGAAGG GTTCCTCTGG GGCGTATCCA CGGGAGCCTT TAACGTGGAA 
GGAGGCTGGG CCGAAGGTGG CAGAGGGCCA AGCATCTGGG ACCACTACAG TAACCTGAAT 
GCTGCGGAGA GCCAAGCGAC AGCAAAGGTG GCCAGTGACA GTTACCACAA ACCAGTTTCT 
GATGTGGCCC TCCTCCGAGG CCTCAGGGCT GACGTGTACA AATTCTCCAT CTCCTGGTCC 
CGCATCTTCC CTTTCGGGCA GAGGACCAGC CCCAACCTCC AAGGTGTTGC CTACTACAAC 
AAACTGATTG ACAGCCTGCT GGACTCCCAG GTGGAGCCCA TGGCTACGCT GTTCCACTGG 
GACCTCCCCC AGGCCCTGCA GGAGCAAGGC GGGTGGCAGA ACGAGAGTGT GGTGGATGCC 
TTCCTGGACT ACGCCGCCTT CTGCTTCTCC ACCTTTGGGG ACCGTGTGAA GCTGTGGGTG 
ACCTTCCATG AGCCCTGGGT GATAAGCTAT GCAGGCTACG GCACCGGGCA GCATGCGCCA 
GCCATCTCTG ACCCAGGAGT GGCTTCCTTT AAGGTGGCTC ACCTGATCCT CAAGGCGCAT 
GCCAGAACTT GGCATCACTA CAACTATCAC CATCGCCAAA AGCAGCAGGG ACGCGTGGGC 
ATTGTGCTCA ATTCAGACTG GGCGGAGCCC CTGGACGGGA AGAGCCCGCA GGACCTTGCT 
GCTGCCGAGC GCTACCTGCA CTTCATGCTA GGCTGGTTTG CACACCCCAT CTTCATAGAT 
GGGGACTACC CTGCTGCCCT TCGGGCCCAG ATCCAGCACA CAAACCAGCA GTGTGGCCGT 
CCTCTGGCCC AACTCCCAGA GTTCACTGAA GCAGAGAAGC GGCTCCTGAA AGGCTCTGCG 
GATTTCCTAG GTCTGTCTCA TTACACATCC CGCCTCATCA GCAAGGCCGG GCAGCAGACC 
TGCATCCCCA GCTATGATAA CATCGGAGGC TTCTCCCAGC ACGTAGATCC CAAGTGGCCC 
CAGACCGCAT CGCCCTGGAT CCGAGTGGTG CCCTGGGGCA TAAGGAGGCT GCTTCGGTTC 
GCTTCCCTGG AATACACGAA AGGAAAACTT CCAATCTTCT TGGCTGGAAA TGGCATGCCA 
ATAGGGGAAG GGTCGGATCT GTTTGATGAC TCAATGAGAG TAAACTACTT AAACTTGTAC 
ATCAACGAGG TGCTCAAGGC TGTCAAAGAG GATTCAGTGG ATGTCCGTTC ATACATTGCT 
CGTTCCCTCA TTGATGGCTA TGAAGGACCC CTGGGCTATA GCCAGAGGTT TGGGCTATAC 
CATGTCAACT TCAATGACAG CAGCAGGCCA CGAACTCCCA GGAAATCTGC CTACTTCTTC 
ACCAGCATCA TTGAAAAGAA CGGTTTTCCT GCCAAGAAAG TCAAAAGAAA CCCACTACCC 
TTGAAAGCAG ACTTCAGCTC CAGAGCCAGG GCTGCCTTCA GCTTTCCATC GGAGGTGCCC 
TCCAAGGCTA AAGTAGTGTG GGAGAAGTTC TCCAGGCAGC CCAAGTTTGA AAGAGACTTA 
TTCTATCATG GCACATTCCG GGATGACTTC CTGTGGGGCG TATCCTCTTC AGCCTATCAA 
ATCGAAGGAG GTTGGGATGC TGATGGCAAA GGCCCCAGCA TCTGGGATAA CTTCACGCAC 
ACCCCAGGGA ACGGTGTGAA AGACAATGCC ACGGGAGACA TAGCGTGTGA CAGCTATCAT 
CAACTGGACG CTGACATAAA CATCCTTCGA ACTCTGAAGG TCAAGTCCTA TCGATTTTCC 
ATCTCCTGGC CTCGAATTTT CCCAACGGGA AGAAACAGTT CCATCAACAA ACAAGGTGTT 
GATTATTACA ACAAACTGAT TGACAGATTG TTGGAGAGCA ACATCTTCCC CATGGTGACA 
CTCTTTCACT GGGACCTGCC CCAGGCCCTC CAGGATATTG GAGGTTGGGA GAACCCTTCT 
TTGATTGAGT TGTTCGATAG CTATGCGGAC TTCTGTTTTA AGACCTTTGG TGACAGAGTC 
AAGTTCTGGA TGACCTTTAA TGAGCCTTGG TGTTCTGCAG TGTTAGGCTA CAGTTCAGGG 
ATATTCCCCC CAAATGTGCA GGACCCGGGC TCACTATCTT ACAAGGTTAG CCATGTAATC 
ATTAAGGCAC ACGCCAGAGT CTACCATACT TATGATGAGA AGTACAGGCA GGAGCAGAAG 
GGGGTCATCT CTCTGAGCCT CAACACTCAC TGGGTCGAAC CCAAGGACCC GGGGGTCCAA 
AGGGACGTGG AAGCCGCTGA CCGGATGCTG CAGTTTAACC TGGGCTGGTT TGCCCACCCT 
ATTTTTAAAA ACGGGGACTA TCCAGATGTC ATGAAATGGA ATGTGGGGAA CAGGAGTGAG 
CTGCAGCACC TGGCCAGCTC CCGCCTGCCC AGCTTCACAG AAGAGGAGAA GAATTACATC 
AGAGGCACCG CCGATGTCTT CTGCATCAAC ACCTACACCT CTGTATTTGC ACAGCATGTC 
ACTCCTAGAC TGAACCCACC CAGCTATGAT AATGACATGG AGTTAAAGGC CAGTGACATG 
AACTCTTCAG CCTTGATCTC CATGATGCAC CAAGACGTGC CCTGGGGGAT GAGGAGGTTG 
CTGAACTGGA TCAAGGAGGA GTATGGCAAC ATTCCCATTT ACATCACTGA GAATGGACAG 
GGGCTGACAA ACCCCACGCT GGATGACACG GAAAGGATAT TCTACCACAA AACCTATATT 
AATGAGGCTC TGAAAGCCTA CAGGCTGGAT GGTGTTGACC TTCGAGGCTA CTCTGCCTGG 
GCGCTGATGG ACAACTTCGA GTGGCTGCAC GGCTACACCA TGAGATTTGG ATTGTATCAC 
GTCGACTTTG ATCATGTGAA CAGACCTCGA ACAGCAAGAG CCTCAGCCAG ATACTATACA 
GAGGTCATTA CCAACAATGG CATGCCCCTA GCCAAGGAGG ATGAGTTCCT GTATGGAGAG 
TTTCCCAAGG GCTTCATCTG GAGTGCAGCG TCTGCTTCCT ATCAGGTTGA AGGTGCGTGG 
AGAGCAGATG GCAAAGGACT TAGTATCTGG GACACATTTT CCCACACTCC ACTGAAGATT 
GGGAATGACG ACAACGGGGA CGTGGCATGT GACAGTTACC ACAAGATTGC TGAGGATGTG 
GTGGCCCTTC AGAACCTGGG CGTGTCCCAC TACCGCTTCT CCATCTCCTG GCCTAGAATC 
CTCCCCGACG GAACCACCAA GTTCATCAAC GAAGCAGGCC TCAACTACTA TGTTCGATTC 
ATAGATGCGC TGCTGGCTGC TGGCATCACA CCACAGGTGA CCATGTATCA CTGGGACCTG 
CCCCAGGCAC TGCAGGACGT TGGGGGCTGG GAGAACGAGA CAGTTGTACA GCGGTTTAAG 
GACTATGCCG ATGTGCTCTT CCGGAGGTTG GGGGACAAGG TCAAGTTCTG GATCACACTG 
AATGAGCCCT TTGTCATTGC GGCCCACGGC TACGGCAGTG GGGTGTCTGC TCCAGGGATC 
TCTTTCAGAC CTGGCACAGC TCCGTACACT GCTGGACACA ACCTAATAAA GGCTCACGCT 
GAGGCTTGGC ATCTCTACAA CAGCACATAC CGTAACAGCC AGGGAGGTGT GATCTCTATC 
ACCATCAGCA GCGACTGGGC CGAGCCCAGA GATCCTTCCA ACCAGGAGGA TGTGGAGGCG 
GCCAGGAGAT ACGTACAGTT TATGGGGGGC TGGTTTGCAC ACCCCATCTT CAAAAACGGA 
GACTACCCTG AGGTGATGAA GACACGGATT CGTGACAGAA GCCTGGCGGC AGGCCTCAAC 
AAGTCCAGGC TCCCTGAATT TACAGAGAGC GAGAAGAAGA AGATCCAGGG CACCTTTGAC 
TTTTTTGGGT TCAACCACTA CACCACTGTC CTAGCCTACA ACCTCAACTA CGCCGCTGCT 
GTTTCGTCTT TTGATGCCGA TAGGGGAGTT GCTTCCATTA CAGACCGCTC CTGGCCAGAC 
TCTGGCTCCT TCTGGCTGAA GATGACCCCT TTTGGCTTCC GGAGGATCTT GAACTGGTTG 
AAGGAGGAGT ACAACAACCC TCTAATTTAT GTCACAGAGA ATGGAGTGTC CCGACGAGGA 
GACCCAGAAC TCAATGACAC CGACAGGATC TACTACCTCC GCAGCTACAT TAATGAAGCC 
CTCAAAGCTG TACGAGATAA GGTGGACCTT CGAGGGTACA CGGTCTGGAG CATCATGGAC 
AACTTTGAAT GGGCCACAGG CTTCGCAGAG AGGTTCGGCG TGCACTTTGT GAACCGCTCT 
GACCCTTCTT TGCCAAGGAT CCCCAAGGCG TCAGCCAAGG TCTACGCCTC CATAGTCCGC 
TGCAATGGCT TTCCTGACCC TGCACAAGGA CCTCATCCTT GTCTCCAGCA ACCAGAAGAT 
GCTGGGCCCA CTGCCAGCCC TGTGAAGTCA GAGGTGCCCT TCCTGGGGCT GATGCTGGGC 
ACGGCAGAAG CACAGACGGC TCTGTATGTC CTCTTCGCTC TTGTGCTGCT TGGAGTTTGC 
AGCGTGGCAT TTCTGTTATA CAAATACTGC AAGCGCTCCA AGCAAGGGAC AACACAGCCA 
GGCCACCACG GTCTGAGTCA AATTTCTTCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074547.1
CDS	16..5811
Translation	MELPWTALFLSTFLLGLSCQGNDWESDRNFISAAGPLTNDLVHNLNHPLGKQGSDLVAGDTDIYVCQQPLPSFLPQYFSSLRASQVTHYKVLLSWAQLLPKGSSKNPDQEAVQCYRQLLQSLKDAQLQPMVVLFHQMPPTSTIQRDGGFADLFADYATLAFQSFGDLVEIWFTFSDLEKVIMGLPHQHLKASGLQTLSDAHRKAFDVYHRKYSSQGGKLSVVLKAEDLPKLLPDPSSAALVKGSVDFLSLDLSYDCQSISTLPQKLSELQNLEPKVKVFVYTLKPQDCPSTGMSPASLLFRLLEAINKDQVQTVGFDVSAFLSCTSSSEESPSCSLTYSSLALQSEQQHETVVSSFPGSAYQRVWAALANQSREERDAFLQDVFPEGFLWGVSTGAFNVEGGWAEGGRGPSIWDHYSNLNAAESQATAKVASDSYHKPVSDVALLRGLRADVYKFSISWSRIFPFGQRTSPNLQGVAYYNKLIDSLLDSQVEPMATLFHWDLPQALQEQGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVISYAGYGTGQHAPAISDPGVASFKVAHLILKAHARTWHHYNYHHRQKQQGRVGIVLNSDWAEPLDGKSPQDLAAAERYLHFMLGWFAHPIFIDGDYPAALRAQIQHTNQQCGRPLAQLPEFTEAEKRLLKGSADFLGLSHYTSRLISKAGQQTCIPSYDNIGGFSQHVDPKWPQTASPWIRVVPWGIRRLLRFASLEYTKGKLPIFLAGNGMPIGEGSDLFDDSMRVNYLNLYINEVLKAVKEDSVDVRSYIARSLIDGYEGPLGYSQRFGLYHVNFNDSSRPRTPRKSAYFFTSIIEKNGFPAKKVKRNPLPLKADFSSRARAAFSFPSEVPSKAKVVWEKFSRQPKFERDLFYHGTFRDDFLWGVSSSAYQIEGGWDADGKGPSIWDNFTHTPGNGVKDNATGDIACDSYHQLDADINILRTLKVKSYRFSISWPRIFPTGRNSSINKQGVDYYNKLIDRLLESNIFPMVTLFHWDLPQALQDIGGWENPSLIELFDSYADFCFKTFGDRVKFWMTFNEPWCSAVLGYSSGIFPPNVQDPGSLSYKVSHVIIKAHARVYHTYDEKYRQEQKGVISLSLNTHWVEPKDPGVQRDVEAADRMLQFNLGWFAHPIFKNGDYPDVMKWNVGNRSELQHLASSRLPSFTEEEKNYIRGTADVFCINTYTSVFAQHVTPRLNPPSYDNDMELKASDMNSSALISMMHQDVPWGMRRLLNWIKEEYGNIPIYITENGQGLTNPTLDDTERIFYHKTYINEALKAYRLDGVDLRGYSAWALMDNFEWLHGYTMRFGLYHVDFDHVNRPRTARASARYYTEVITNNGMPLAKEDEFLYGEFPKGFIWSAASASYQVEGAWRADGKGLSIWDTFSHTPLKIGNDDNGDVACDSYHKIAEDVVALQNLGVSHYRFSISWPRILPDGTTKFINEAGLNYYVRFIDALLAAGITPQVTMYHWDLPQALQDVGGWENETVVQRFKDYADVLFRRLGDKVKFWITLNEPFVIAAHGYGSGVSAPGISFRPGTAPYTAGHNLIKAHAEAWHLYNSTYRNSQGGVISITISSDWAEPRDPSNQEDVEAARRYVQFMGGWFAHPIFKNGDYPEVMKTRIRDRSLAAGLNKSRLPEFTESEKKKIQGTFDFFGFNHYTTVLAYNLNYAAAVSSFDADRGVASITDRSWPDSGSFWLKMTPFGFRRILNWLKEEYNNPLIYVTENGVSRRGDPELNDTDRIYYLRSYINEALKAVRDKVDLRGYTVWSIMDNFEWATGFAERFGVHFVNRSDPSLPRIPKASAKVYASIVRCNGFPDPAQGPHPCLQQPEDAGPTASPVKSEVPFLGLMLGTAEAQTALYVLFALVLLGVCSVAFLLYKYCKRSKQGTTQPGHHGLSQISSF

Target ORF information:

RefSeq Version	NM_001081078.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lactase (Lct), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081078.2

ORF Insert Sequence:

ATGGAGCTGC CTTGGACAGC ACTCTTCCTC AGTACCTTCC TCCTGGGTCT TTCATGCCAG 
GGGAATGACT GGGAATCTGA CAGAAATTTT ATTTCAGCTG CTGGTCCTCT AACAAATGAC 
TTAGTGCACA ATCTGAACCA TCCGCTGGGA AAGCAGGGCT CTGATTTGGT AGCTGGTGAT 
ACTGACATCT ATGTCTGCCA GCAGCCACTG CCCTCCTTCT TGCCTCAGTA CTTCAGCAGT 
CTTCGGGCCA GCCAGGTTAC CCATTACAAA GTACTTCTGT CATGGGCACA ACTTCTCCCA 
AAAGGAAGCT CCAAGAATCC AGACCAGGAA GCAGTGCAGT GCTACCGACA ACTCCTCCAG 
TCCCTGAAGG ATGCCCAGCT TCAGCCCATG GTGGTCCTGT TCCACCAGAT GCCACCTACC 
AGCACCATCC AGAGAGACGG GGGCTTTGCT GACCTCTTTG CTGATTATGC CACATTGGCT 
TTTCAGTCCT TTGGGGACCT TGTTGAGATC TGGTTCACCT TCAGTGACTT GGAGAAAGTG 
ATCATGGGTC TTCCCCACCA GCATTTGAAA GCTTCTGGTC TCCAAACCCT CAGCGATGCC 
CACAGGAAAG CCTTTGACGT TTACCACAGA AAGTACTCTT CTCAGGGAGG AAAGCTGTCT 
GTGGTCCTGA AGGCTGAAGA CCTCCCCAAG CTTCTGCCAG ACCCATCCTC AGCGGCTCTC 
GTCAAGGGCT CCGTGGATTT CCTCTCTCTT GATTTGTCTT ATGACTGCCA AAGTATATCG 
ACTCTACCAC AGAAGCTGAG TGAATTGCAG AACCTTGAGC CCAAAGTGAA AGTTTTCGTC 
TACACCCTAA AACCTCAGGA CTGCCCATCG ACTGGAATGA GCCCCGCCTC ACTGCTCTTC 
AGACTCCTGG AAGCCATAAA TAAAGACCAA GTCCAGACTG TTGGGTTTGA TGTCAGTGCC 
TTCCTGAGCT GTACGTCCAG TTCTGAGGAG AGCCCATCTT GTTCTTTAAC CTACAGTAGC 
CTGGCCCTTC AGAGCGAGCA GCAGCATGAG ACAGTTGTGT CCTCATTCCC TGGGTCGGCC 
TACCAGAGAG TCTGGGCAGC CTTGGCTAAC CAGTCTAGGG AGGAAAGGGA CGCTTTCCTG 
CAAGATGTAT TTCCAGAAGG GTTCCTCTGG GGCGTATCCA CGGGAGCCTT TAACGTGGAA 
GGAGGCTGGG CCGAAGGTGG CAGAGGGCCA AGCATCTGGG ACCACTACAG TAACCTGAAT 
GCTGCGGAGA GCCAAGCGAC AGCAAAGGTG GCCAGTGACA GTTACCACAA ACCAGTTTCT 
GATGTGGCCC TCCTCCGAGG CCTCAGGGCT GACGTGTACA AATTCTCCAT CTCCTGGTCC 
CGCATCTTCC CTTTCGGGCA GAGGACCAGC CCCAACCTCC AAGGTGTTGC CTACTACAAC 
AAACTGATTG ACAGCCTGCT GGACTCCCAG GTGGAGCCCA TGGCTACGCT GTTCCACTGG 
GACCTCCCCC AGGCCCTGCA GGAGCAAGGC GGGTGGCAGA ACGAGAGTGT GGTGGATGCC 
TTCCTGGACT ACGCCGCCTT CTGCTTCTCC ACCTTTGGGG ACCGTGTGAA GCTGTGGGTG 
ACCTTCCATG AGCCCTGGGT GATAAGCTAT GCAGGCTACG GCACCGGGCA GCATGCGCCA 
GCCATCTCTG ACCCAGGAGT GGCTTCCTTT AAGGTGGCTC ACCTGATCCT CAAGGCGCAT 
GCCAGAACTT GGCATCACTA CAACTATCAC CATCGCCAAA AGCAGCAGGG ACGCGTGGGC 
ATTGTGCTCA ATTCAGACTG GGCGGAGCCC CTGGACGGGA AGAGCCCGCA GGACCTTGCT 
GCTGCCGAGC GCTACCTGCA CTTCATGCTA GGCTGGTTTG CACACCCCAT CTTCATAGAT 
GGGGACTACC CTGCTGCCCT TCGGGCCCAG ATCCAGCACA CAAACCAGCA GTGTGGCCGT 
CCTCTGGCCC AACTCCCAGA GTTCACTGAA GCAGAGAAGC GGCTCCTGAA AGGCTCTGCG 
GATTTCCTAG GTCTGTCTCA TTACACATCC CGCCTCATCA GCAAGGCCGG GCAGCAGACC 
TGCATCCCCA GCTATGATAA CATCGGAGGC TTCTCCCAGC ACGTAGATCC CAAGTGGCCC 
CAGACCGCAT CGCCCTGGAT CCGAGTGGTG CCCTGGGGCA TAAGGAGGCT GCTTCGGTTC 
GCTTCCCTGG AATACACGAA AGGAAAACTT CCAATCTTCT TGGCTGGAAA TGGCATGCCA 
ATAGGGGAAG GGTCGGATCT GTTTGATGAC TCAATGAGAG TAAACTACTT AAACTTGTAC 
ATCAACGAGG TGCTCAAGGC TGTCAAAGAG GATTCAGTGG ATGTCCGTTC ATACATTGCT 
CGTTCCCTCA TTGATGGCTA TGAAGGACCC CTGGGCTATA GCCAGAGGTT TGGGCTATAC 
CATGTCAACT TCAATGACAG CAGCAGGCCA CGAACTCCCA GGAAATCTGC CTACTTCTTC 
ACCAGCATCA TTGAAAAGAA CGGTTTTCCT GCCAAGAAAG TCAAAAGAAA CCCACTACCC 
TTGAAAGCAG ACTTCAGCTC CAGAGCCAGG GCTGCCTTCA GCTTTCCATC GGAGGTGCCC 
TCCAAGGCTA AAGTAGTGTG GGAGAAGTTC TCCAGGCAGC CCAAGTTTGA AAGAGACTTA 
TTCTATCATG GCACATTCCG GGATGACTTC CTGTGGGGCG TATCCTCTTC AGCCTATCAA 
ATCGAAGGAG GTTGGGATGC TGATGGCAAA GGCCCCAGCA TCTGGGATAA CTTCACGCAC 
ACCCCAGGGA ACGGTGTGAA AGACAATGCC ACGGGAGACA TAGCGTGTGA CAGCTATCAT 
CAACTGGACG CTGACATAAA CATCCTTCGA ACTCTGAAGG TCAAGTCCTA TCGATTTTCC 
ATCTCCTGGC CTCGAATTTT CCCAACGGGA AGAAACAGTT CCATCAACAA ACAAGGTGTT 
GATTATTACA ACAAACTGAT TGACAGATTG TTGGAGAGCA ACATCTTCCC CATGGTGACA 
CTCTTTCACT GGGACCTGCC CCAGGCCCTC CAGGATATTG GAGGTTGGGA GAACCCTTCT 
TTGATTGAGT TGTTCGATAG CTATGCGGAC TTCTGTTTTA AGACCTTTGG TGACAGAGTC 
AAGTTCTGGA TGACCTTTAA TGAGCCTTGG TGTTCTGCAG TGTTAGGCTA CAGTTCAGGG 
ATATTCCCCC CAAATGTGCA GGACCCGGGC TCACTATCTT ACAAGGTTAG CCATGTAATC 
ATTAAGGCAC ACGCCAGAGT CTACCATACT TATGATGAGA AGTACAGGCA GGAGCAGAAG 
GGGGTCATCT CTCTGAGCCT CAACACTCAC TGGGTCGAAC CCAAGGACCC GGGGGTCCAA 
AGGGACGTGG AAGCCGCTGA CCGGATGCTG CAGTTTAACC TGGGCTGGTT TGCCCACCCT 
ATTTTTAAAA ACGGGGACTA TCCAGATGTC ATGAAATGGA ATGTGGGGAA CAGGAGTGAG 
CTGCAGCACC TGGCCAGCTC CCGCCTGCCC AGCTTCACAG AAGAGGAGAA GAATTACATC 
AGAGGCACCG CCGATGTCTT CTGCATCAAC ACCTACACCT CTGTATTTGC ACAGCATGTC 
ACTCCTAGAC TGAACCCACC CAGCTATGAT AATGACATGG AGTTAAAGGC CAGTGACATG 
AACTCTTCAG CCTTGATCTC CATGATGCAC CAAGACGTGC CCTGGGGGAT GAGGAGGTTG 
CTGAACTGGA TCAAGGAGGA GTATGGCAAC ATTCCCATTT ACATCACTGA GAATGGACAG 
GGGCTGACAA ACCCCACGCT GGATGACACG GAAAGGATAT TCTACCACAA AACCTATATT 
AATGAGGCTC TGAAAGCCTA CAGGCTGGAT GGTGTTGACC TTCGAGGCTA CTCTGCCTGG 
GCGCTGATGG ACAACTTCGA GTGGCTGCAC GGCTACACCA TGAGATTTGG ATTGTATCAC 
GTCGACTTTG ATCATGTGAA CAGACCTCGA ACAGCAAGAG CCTCAGCCAG ATACTATACA 
GAGGTCATTA CCAACAATGG CATGCCCCTA GCCAAGGAGG ATGAGTTCCT GTATGGAGAG 
TTTCCCAAGG GCTTCATCTG GAGTGCAGCG TCTGCTTCCT ATCAGGTTGA AGGTGCGTGG 
AGAGCAGATG GCAAAGGACT TAGTATCTGG GACACATTTT CCCACACTCC ACTGAAGATT 
GGGAATGACG ACAACGGGGA CGTGGCATGT GACAGTTACC ACAAGATTGC TGAGGATGTG 
GTGGCCCTTC AGAACCTGGG CGTGTCCCAC TACCGCTTCT CCATCTCCTG GCCTAGAATC 
CTCCCCGACG GAACCACCAA GTTCATCAAC GAAGCAGGCC TCAACTACTA TGTTCGATTC 
ATAGATGCGC TGCTGGCTGC TGGCATCACA CCACAGGTGA CCATGTATCA CTGGGACCTG 
CCCCAGGCAC TGCAGGACGT TGGGGGCTGG GAGAACGAGA CAGTTGTACA GCGGTTTAAG 
GACTATGCCG ATGTGCTCTT CCGGAGGTTG GGGGACAAGG TCAAGTTCTG GATCACACTG 
AATGAGCCCT TTGTCATTGC GGCCCACGGC TACGGCAGTG GGGTGTCTGC TCCAGGGATC 
TCTTTCAGAC CTGGCACAGC TCCGTACACT GCTGGACACA ACCTAATAAA GGCTCACGCT 
GAGGCTTGGC ATCTCTACAA CAGCACATAC CGTAACAGCC AGGGAGGTGT GATCTCTATC 
ACCATCAGCA GCGACTGGGC CGAGCCCAGA GATCCTTCCA ACCAGGAGGA TGTGGAGGCG 
GCCAGGAGAT ACGTACAGTT TATGGGGGGC TGGTTTGCAC ACCCCATCTT CAAAAACGGA 
GACTACCCTG AGGTGATGAA GACACGGATT CGTGACAGAA GCCTGGCGGC AGGCCTCAAC 
AAGTCCAGGC TCCCTGAATT TACAGAGAGC GAGAAGAAGA AGATCCAGGG CACCTTTGAC 
TTTTTTGGGT TCAACCACTA CACCACTGTC CTAGCCTACA ACCTCAACTA CGCCGCTGCT 
GTTTCGTCTT TTGATGCCGA TAGGGGAGTT GCTTCCATTA CAGACCGCTC CTGGCCAGAC 
TCTGGCTCCT TCTGGCTGAA GATGACCCCT TTTGGCTTCC GGAGGATCTT GAACTGGTTG 
AAGGAGGAGT ACAACAACCC TCTAATTTAT GTCACAGAGA ATGGAGTGTC CCGACGAGGA 
GACCCAGAAC TCAATGACAC CGACAGGATC TACTACCTCC GCAGCTACAT TAATGAAGCC 
CTCAAAGCTG TACGAGATAA GGTGGACCTT CGAGGGTACA CGGTCTGGAG CATCATGGAC 
AACTTTGAAT GGGCCACAGG CTTCGCAGAG AGGTTCGGCG TGCACTTTGT GAACCGCTCT 
GACCCTTCTT TGCCAAGGAT CCCCAAGGCG TCAGCCAAGG TCTACGCCTC CATAGTCCGC 
TGCAATGGCT TTCCTGACCC TGCACAAGGA CCTCATCCTT GTCTCCAGCA ACCAGAAGAT 
GCTGGGCCCA CTGCCAGCCC TGTGAAGTCA GAGGTGCCCT TCCTGGGGCT GATGCTGGGC 
ACGGCAGAAG CACAGACGGC TCTGTATGTC CTCTTCGCTC TTGTGCTGCT TGGAGTTTGC 
AGCGTGGCAT TTCTGTTATA CAAATACTGC AAGCGCTCCA AGCAAGGGAC AACACAGCCA 
GGCCACCACG GTCTGAGTCA AATTTCTTCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Transcriptional heterogeneity in the lactase gene within cell-type is linked to the epigenome.
Scientific reports741843(2017 Jan)
Oh E,Jeremian R,Oh G,Groot D,Susic M,Lee K,Foy K,Laird PW,Petronis A,Labrie V

Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging.
Nature structural & molecular biology23(6)566-73(2016 06)
Labrie V,Buske OJ,Oh E,Jeremian R,Ptak C,Gasi?nas G,Maleckas A,Petereit R,?virbliene A,Adamonis K,Kriukien? E,Koncevi?ius K,Gordevi?ius J,Nair A,Zhang A,Ebrahimi S,Oh G,?ik?nys V,Kup?inskas L,Brudno M,Petronis A

GATA4 and GATA6 regulate intestinal epithelial cytodifferentiation during development.
Developmental biology392(2)283-94(2014 Aug)
Walker EM,Thompson CA,Battle MA