Pde6a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde6a
Entrez Gene ID	225600
Full Name	phosphodiesterase 6A, cGMP-specific, rod, alpha
Synonyms	Pdea,nmf282
General protein information	Preferred Names phosphodiesterase 6A, cGMP-specific, rod, alpha Names rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha GMP-PDE alpha cGMP phosphodiesterase alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 E1\|18 34.41 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_146086.2	NP_666198.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
XM_006525880.4	XP_006525943.2	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
XM_006525880.3	XP_006525943.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
NM_146086.3	NP_666198.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE6A	NP_000431.2
Pan troglodytes (chimpanzee)	PDE6A	XP_518030.2
Mus musculus (house mouse)	Pde6a	NP_666198.1
Rattus norvegicus (Norway rat)	Pde6a	NP_001100856.1
Xenopus tropicalis (tropical clawed frog)	pde6a	XP_002936750.2
Canis lupus familiaris (dog)	PDE6A	NP_001003073.1
Danio rerio (zebrafish)	pde6a	NP_001007161.1
Bos taurus (cattle)	PDE6A	NP_001001526.2
	PDE6A	XP_001107349.1

Gene Ontology
Bibliography

Related articles in PubMed

Ciliopathy-associated IQCB1/NPHP5 protein is required for mouse photoreceptor outer segment formation.
Ronquillo CC, Hanke-Gogokhia C, Revelo MP, Frederick JM, Jiang L, Baehr W
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(10)3400-3412(2016 10)

Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.
Wong BH, Chan JP, Cazenave-Gassiot A, Poh RW, Foo JC, Galam DL, Ghosh S, Nguyen LN, Barathi VA, Yeo SW, Luu CD, Wenk MR, Silver DL
The Journal of biological chemistry291(20)10501-14(2016 May)

Efficacy of PARP inhibition in Pde6a mutant mouse models for retinitis pigmentosa depends on the quality and composition of individual human mutations.
Jiao K, Sahaboglu A, Zrenner E, Ueffing M, Ekstr?m PA, Paquet-Durand F
Cell death discovery216040(2016)

Retinitis pigmentosa: impact of different Pde6a point mutations on the disease phenotype.
Sothilingam V, Garcia Garrido M, Jiao K, Buena-Atienza E, Sahaboglu A, Trifunovi? D, Balendran S, Koepfli T, M?hlfriedel R, Sch?n C, Biel M, Heckmann A, Beck SC, Michalakis S, Wissinger B, Seeliger MW, Paquet-Durand F
Human molecular genetics24(19)5486-99(2015 Oct)

Alternative Splicing Shapes the Phenotype of a Mutation in BBS8 To Cause Nonsyndromic Retinitis Pigmentosa.
Murphy D, Singh R, Kolandaivelu S, Ramamurthy V, Stoilov P
Molecular and cellular biology35(10)1860-70(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study confirms the severity of different Pde6a mutations and indicates that compound heterozygous mutants behave like intermediates of the respective homozygous situations homologous to a case of human retinitis pigmentosa.
Title: Retinitis pigmentosa: impact of different Pde6a point mutations on the disease phenotype.

cone PDE6alpha' can functionally substitute for rod PDEalphabeta in vivo, conferring treated rods with distinct physiological properties.
Title: Cone phosphodiesterase-6?' restores rod function and confers distinct physiological properties in the rod phosphodiesterase-6?-deficient rd10 mouse.

A new mutation in Pde6a was identifies in a mouse model of retinal degeneration.
Title: Translational vision research models program.

Either rod or cone PDE6 can effectively couple to the cone phototransduction pathway to mediate visual signaling.
Title: Rod phosphodiesterase-6 (PDE6) catalytic subunits restore cone function in a mouse model lacking cone PDE6 catalytic subunit.

Phosphodiesterase 6 is involved in the recovery-accelerating effect of red light on the disrupted epidermal permeability barrier.
Title: Phosphodiesterase inhibitors block the acceleration of skin permeability barrier repair by red light.

The following Pde6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14480	NM_146086.3 Latest version!	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00
OMu54548	XM_006525880.3 Latest version!	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu87385	XM_006525880.4 Latest version!	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu14480	NM_146086.2	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde6a ( NM_146086.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666198.1 Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), mRNA.

Price & Availability↑

CloneID	OMu14480
Clone ID Related Accession (Same CDS sequence)	NM_146086.2 , NM_146086.3
Accession Version	NM_146086.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Mus musculus(house mouse)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044892.1. On or before May 4, 2006 this sequence version replaced XM_001005843.1, NM_146086.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044892.1, X60664.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849376 [ECO:0000350] ##Evidence-Data-END##

ATGGGTGAGG TGACAGCAGA GGAGGTAGAA AAGTTCCTGG ATTCAAATAT TGGCTTTGCC 
AAACAGTACT ATAACTTTCA CTACCGGGGG AAGGTCATCT CAGACCTCCT CGGGGCCAAG 
GAGGCAGCCG TGGACTTCAG CAACTACCAC GATGTGAACA GCGTAGAGGA GAGTGAGATC 
ATCTTTGACC TCCTGCGGGA CGTTCAGGAG AACTTACAGG CTGAGAAATG CACATTCAAT 
GTCATGAAGA AGCTCTGCTT CCTCCTGCGG GCTGACCGCA TGAGCCTGTT CATGTACAGG 
ACCCGCAACG GGATCGCCGA GTTGGCCACT AGGCTCTTCA ATGTCCACAA GGATGCCGTC 
CTAGAGGACT GCTTGGTGAT GCCCGACTCC GAGATTGTCT TCCCTCTGGA CATGGGTGTC 
GTGGGCCACG TCGCACACTC CAAAAAGATT GCCAATGTCC CCAACACAGA AGAGGATGAG 
CATTTCTGTG ACTTCGTGGA CAATCTCACA GAATATCAGA CCAAAAACAT CCTGGCTTCC 
CCCATCATGA ATGGGAAGGA TGTGGTAGCC ATAATCATGG CTGTGAATAA AATAGATGAA 
CCCCACTTCA CCAAGAGAGA TGAAGAGATT CTTCTCAAGT ACCTCAACTT TGTGAACCTG 
ATCATGAAGG TATTCCACCT GAGCTACCTG CACAACTGTG AGACTCGTCG CGGCCAGATA 
TTGCTGTGGT CTGGGAGCAA GGTCTTTGAG GAGCTCACGG ATATAGAGAG GCAGTTCCAC 
AAGGCCCTGT ACACGGTCCG GGCTTTCCTC AACTGTGACA GATACTCCGT AGGACTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TTCTGATGGG CGAGGCTCCA 
GCTTACTCTG GTCCCAGGAC TCCAGACGGA AGGGAAATTA ACTTCTACAA GGTCATTGAC 
TACATCCTGC ACGGCAAAGA AGACATCAAA GTCATCCCGA ACCCACCCGC TGACCACTGG 
GCTCTAGTGA GTGGTCTCCC CACTTACGTG GCTCAAAATG GTCTGATCTG CAATATAATG 
AATGCGCCTG CAGAGGACTT TTTTGAATTC CAGAAAGAGC CTCTGGATGA GTCTGGGTGG 
ATGATTAAAA ATGTACTCTC CATGCCCATC GTCAACAAGA AGGAAGAGAT CGTCGGCGTG 
GCCACATTTT ACAACCGCAA AGATGGGAAG CCCTTCGACG ATATGGACGA GACCCTCATG 
GAGTCTTTGA CTCAGTTTCT GGGATGGTCA GTCTTAAACC CTGACACCTA CGAGTCCATG 
AACAAGCTCG AGAACAGGAA GGATATCTTC CAGGACATCG TGAAATATCA CGTGAAGTGT 
GATAACGAAG AAATCCAGAA GATCTTGAAA ACCAGAGAGG TGTACGGCAA AGAGCCGTGG 
GAATGCGAGG AGGAGGAGCT GGCTGAGATC CTGCAAGGAG AACTTCCAGA CGCGGAGTCA 
TACGAAATCA ACAAGTTCCA CTTCAGCGAC CTGCCACTCA CGGAGCTGGA GCTGGTGAAG 
TGCGGCATCC AGATGTACTA CGAGCTCAGA GTGGTGGACA AGTTCCACAT CCCGCAAGAG 
GCCCTGGTGC GCTTCATGTA TTCGCTAAGC AAAGGCTACC GGAGAATCAC TTACCACAAC 
TGGCGGCATG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC AGGAAAGCTG 
AAACGGTACT TCACTGATCT AGAGGCCTTG GCCATGGTCA CTGCTGCCTT CTGTCATGAC 
ATCGACCACA GAGGCACGAA CAACCTCTAC CAGATGAAAT CACAGAACCC CCTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGGCAT CATTTGGAGT TTGGCAAAAC ACTCCTGAGA 
GATGAGAGCC TGAATATCTT CCAGAACCTG AATCGCCGGC AGCATGAGCA CGCGATCCAC 
ATGATGGACA TCGCGATCAT TGCCACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACCATG 
TTCCAGAAGA TTGTGGATCA GTCAAAGACA TATGAGAGTA CCCAGGAGTG GACCCAGTAC 
ATGATGCTGG AGCAGACACG GAAGGAAATT GTGATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAACC CTGGGAGGTA CAGAGCAAGG TGGCTCTGCT GGTGGCTGCT 
GAATTCTGGG AGCAAGGTGA CCTGGAGCGC ACAGTGCTGC AGCAGAATCC CATTCCCATG 
ATGGACAGAA ACAAGGCGGA TGAGCTCCCC AAGCTTCAAG TCGGCTTCAT CGACTTTGTG 
TGCACTTTTG TCTATAAGGA GTTCTCCCGA TTTCATGAGG AGATTACACC CATGCTGGAT 
GGGATCACTA ACAACCGCAA GGAATGGAAG GCGCTGGCTG ATGAGTACGA AGCCAAGATG 
AAGGCCCTGG AGGAGGAGAA GCAGAAGCAG CAGGCAGCCA AGCAAGCTGC TTCCGGGAAC 
CAGCCAGGAG GGAACCCCAC TCCAGGGGGT GCACCTGCAT CTAAGTCCTG TTGCATCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666198.1
CDS	107..2689
Translation	MGEVTAEEVEKFLDSNIGFAKQYYNFHYRGKVISDLLGAKEAAVDFSNYHDVNSVEESEIIFDLLRDVQENLQAEKCTFNVMKKLCFLLRADRMSLFMYRTRNGIAELATRLFNVHKDAVLEDCLVMPDSEIVFPLDMGVVGHVAHSKKIANVPNTEEDEHFCDFVDNLTEYQTKNILASPIMNGKDVVAIIMAVNKIDEPHFTKRDEEILLKYLNFVNLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPAYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYVAQNGLICNIMNAPAEDFFEFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDDMDETLMESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQGELPDAESYEINKFHFSDLPLTELELVKCGIQMYYELRVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESTQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYEAKMKALEEEKQKQQAAKQAASGNQPGGNPTPGGAPASKSCCIQ

Target ORF information:

RefSeq Version	NM_146086.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146086.2

ORF Insert Sequence:

ATGGGTGAGG TGACAGCAGA GGAGGTAGAA AAGTTCCTGG ATTCAAATAT TGGCTTTGCC 
AAACAGTACT ATAACTTTCA CTACCGGGGG AAGGTCATCT CAGACCTCCT CGGGGCCAAG 
GAGGCAGCCG TGGACTTCAG CAACTACCAC GATGTGAACA GCGTAGAGGA GAGTGAGATC 
ATCTTTGACC TCCTGCGGGA CGTTCAGGAG AACTTACAGG CTGAGAAATG CACATTCAAT 
GTCATGAAGA AGCTCTGCTT CCTCCTGCGG GCTGACCGCA TGAGCCTGTT CATGTACAGG 
ACCCGCAACG GGATCGCCGA GTTGGCCACT AGGCTCTTCA ATGTCCACAA GGATGCCGTC 
CTAGAGGACT GCTTGGTGAT GCCCGACTCC GAGATTGTCT TCCCTCTGGA CATGGGTGTC 
GTGGGCCACG TCGCACACTC CAAAAAGATT GCCAATGTCC CCAACACAGA AGAGGATGAG 
CATTTCTGTG ACTTCGTGGA CAATCTCACA GAATATCAGA CCAAAAACAT CCTGGCTTCC 
CCCATCATGA ATGGGAAGGA TGTGGTAGCC ATAATCATGG CTGTGAATAA AATAGATGAA 
CCCCACTTCA CCAAGAGAGA TGAAGAGATT CTTCTCAAGT ACCTCAACTT TGTGAACCTG 
ATCATGAAGG TATTCCACCT GAGCTACCTG CACAACTGTG AGACTCGTCG CGGCCAGATA 
TTGCTGTGGT CTGGGAGCAA GGTCTTTGAG GAGCTCACGG ATATAGAGAG GCAGTTCCAC 
AAGGCCCTGT ACACGGTCCG GGCTTTCCTC AACTGTGACA GATACTCCGT AGGACTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TTCTGATGGG CGAGGCTCCA 
GCTTACTCTG GTCCCAGGAC TCCAGACGGA AGGGAAATTA ACTTCTACAA GGTCATTGAC 
TACATCCTGC ACGGCAAAGA AGACATCAAA GTCATCCCGA ACCCACCCGC TGACCACTGG 
GCTCTAGTGA GTGGTCTCCC CACTTACGTG GCTCAAAATG GTCTGATCTG CAATATAATG 
AATGCGCCTG CAGAGGACTT TTTTGAATTC CAGAAAGAGC CTCTGGATGA GTCTGGGTGG 
ATGATTAAAA ATGTACTCTC CATGCCCATC GTCAACAAGA AGGAAGAGAT CGTCGGCGTG 
GCCACATTTT ACAACCGCAA AGATGGGAAG CCCTTCGACG ATATGGACGA GACCCTCATG 
GAGTCTTTGA CTCAGTTTCT GGGATGGTCA GTCTTAAACC CTGACACCTA CGAGTCCATG 
AACAAGCTCG AGAACAGGAA GGATATCTTC CAGGACATCG TGAAATATCA CGTGAAGTGT 
GATAACGAAG AAATCCAGAA GATCTTGAAA ACCAGAGAGG TGTACGGCAA AGAGCCGTGG 
GAATGCGAGG AGGAGGAGCT GGCTGAGATC CTGCAAGGAG AACTTCCAGA CGCGGAGTCA 
TACGAAATCA ACAAGTTCCA CTTCAGCGAC CTGCCACTCA CGGAGCTGGA GCTGGTGAAG 
TGCGGCATCC AGATGTACTA CGAGCTCAGA GTGGTGGACA AGTTCCACAT CCCGCAAGAG 
GCCCTGGTGC GCTTCATGTA TTCGCTAAGC AAAGGCTACC GGAGAATCAC TTACCACAAC 
TGGCGGCATG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC AGGAAAGCTG 
AAACGGTACT TCACTGATCT AGAGGCCTTG GCCATGGTCA CTGCTGCCTT CTGTCATGAC 
ATCGACCACA GAGGCACGAA CAACCTCTAC CAGATGAAAT CACAGAACCC CCTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGGCAT CATTTGGAGT TTGGCAAAAC ACTCCTGAGA 
GATGAGAGCC TGAATATCTT CCAGAACCTG AATCGCCGGC AGCATGAGCA CGCGATCCAC 
ATGATGGACA TCGCGATCAT TGCCACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACCATG 
TTCCAGAAGA TTGTGGATCA GTCAAAGACA TATGAGAGTA CCCAGGAGTG GACCCAGTAC 
ATGATGCTGG AGCAGACACG GAAGGAAATT GTGATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAACC CTGGGAGGTA CAGAGCAAGG TGGCTCTGCT GGTGGCTGCT 
GAATTCTGGG AGCAAGGTGA CCTGGAGCGC ACAGTGCTGC AGCAGAATCC CATTCCCATG 
ATGGACAGAA ACAAGGCGGA TGAGCTCCCC AAGCTTCAAG TCGGCTTCAT CGACTTTGTG 
TGCACTTTTG TCTATAAGGA GTTCTCCCGA TTTCATGAGG AGATTACACC CATGCTGGAT 
GGGATCACTA ACAACCGCAA GGAATGGAAG GCGCTGGCTG ATGAGTACGA AGCCAAGATG 
AAGGCCCTGG AGGAGGAGAA GCAGAAGCAG CAGGCAGCCA AGCAAGCTGC TTCCGGGAAC 
CAGCCAGGAG GGAACCCCAC TCCAGGGGGT GCACCTGCAT CTAAGTCCTG TTGCATCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6a ( NM_146086.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666198.1 Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), mRNA.

Price & Availability↑

CloneID	OMu14480
Clone ID Related Accession (Same CDS sequence)	NM_146086.2 , NM_146086.3
Accession Version	NM_146086.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC148012.5 and BC044892.1. On Jul 20, 2018 this sequence version replaced NM_146086.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044892.1, X60664.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849376 [ECO:0000350] ##Evidence-Data-END##

ATGGGTGAGG TGACAGCAGA GGAGGTAGAA AAGTTCCTGG ATTCAAATAT TGGCTTTGCC 
AAACAGTACT ATAACTTTCA CTACCGGGGG AAGGTCATCT CAGACCTCCT CGGGGCCAAG 
GAGGCAGCCG TGGACTTCAG CAACTACCAC GATGTGAACA GCGTAGAGGA GAGTGAGATC 
ATCTTTGACC TCCTGCGGGA CGTTCAGGAG AACTTACAGG CTGAGAAATG CACATTCAAT 
GTCATGAAGA AGCTCTGCTT CCTCCTGCGG GCTGACCGCA TGAGCCTGTT CATGTACAGG 
ACCCGCAACG GGATCGCCGA GTTGGCCACT AGGCTCTTCA ATGTCCACAA GGATGCCGTC 
CTAGAGGACT GCTTGGTGAT GCCCGACTCC GAGATTGTCT TCCCTCTGGA CATGGGTGTC 
GTGGGCCACG TCGCACACTC CAAAAAGATT GCCAATGTCC CCAACACAGA AGAGGATGAG 
CATTTCTGTG ACTTCGTGGA CAATCTCACA GAATATCAGA CCAAAAACAT CCTGGCTTCC 
CCCATCATGA ATGGGAAGGA TGTGGTAGCC ATAATCATGG CTGTGAATAA AATAGATGAA 
CCCCACTTCA CCAAGAGAGA TGAAGAGATT CTTCTCAAGT ACCTCAACTT TGTGAACCTG 
ATCATGAAGG TATTCCACCT GAGCTACCTG CACAACTGTG AGACTCGTCG CGGCCAGATA 
TTGCTGTGGT CTGGGAGCAA GGTCTTTGAG GAGCTCACGG ATATAGAGAG GCAGTTCCAC 
AAGGCCCTGT ACACGGTCCG GGCTTTCCTC AACTGTGACA GATACTCCGT AGGACTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TTCTGATGGG CGAGGCTCCA 
GCTTACTCTG GTCCCAGGAC TCCAGACGGA AGGGAAATTA ACTTCTACAA GGTCATTGAC 
TACATCCTGC ACGGCAAAGA AGACATCAAA GTCATCCCGA ACCCACCCGC TGACCACTGG 
GCTCTAGTGA GTGGTCTCCC CACTTACGTG GCTCAAAATG GTCTGATCTG CAATATAATG 
AATGCGCCTG CAGAGGACTT TTTTGAATTC CAGAAAGAGC CTCTGGATGA GTCTGGGTGG 
ATGATTAAAA ATGTACTCTC CATGCCCATC GTCAACAAGA AGGAAGAGAT CGTCGGCGTG 
GCCACATTTT ACAACCGCAA AGATGGGAAG CCCTTCGACG ATATGGACGA GACCCTCATG 
GAGTCTTTGA CTCAGTTTCT GGGATGGTCA GTCTTAAACC CTGACACCTA CGAGTCCATG 
AACAAGCTCG AGAACAGGAA GGATATCTTC CAGGACATCG TGAAATATCA CGTGAAGTGT 
GATAACGAAG AAATCCAGAA GATCTTGAAA ACCAGAGAGG TGTACGGCAA AGAGCCGTGG 
GAATGCGAGG AGGAGGAGCT GGCTGAGATC CTGCAAGGAG AACTTCCAGA CGCGGAGTCA 
TACGAAATCA ACAAGTTCCA CTTCAGCGAC CTGCCACTCA CGGAGCTGGA GCTGGTGAAG 
TGCGGCATCC AGATGTACTA CGAGCTCAGA GTGGTGGACA AGTTCCACAT CCCGCAAGAG 
GCCCTGGTGC GCTTCATGTA TTCGCTAAGC AAAGGCTACC GGAGAATCAC TTACCACAAC 
TGGCGGCATG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC AGGAAAGCTG 
AAACGGTACT TCACTGATCT AGAGGCCTTG GCCATGGTCA CTGCTGCCTT CTGTCATGAC 
ATCGACCACA GAGGCACGAA CAACCTCTAC CAGATGAAAT CACAGAACCC CCTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGGCAT CATTTGGAGT TTGGCAAAAC ACTCCTGAGA 
GATGAGAGCC TGAATATCTT CCAGAACCTG AATCGCCGGC AGCATGAGCA CGCGATCCAC 
ATGATGGACA TCGCGATCAT TGCCACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACCATG 
TTCCAGAAGA TTGTGGATCA GTCAAAGACA TATGAGAGTA CCCAGGAGTG GACCCAGTAC 
ATGATGCTGG AGCAGACACG GAAGGAAATT GTGATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAACC CTGGGAGGTA CAGAGCAAGG TGGCTCTGCT GGTGGCTGCT 
GAATTCTGGG AGCAAGGTGA CCTGGAGCGC ACAGTGCTGC AGCAGAATCC CATTCCCATG 
ATGGACAGAA ACAAGGCGGA TGAGCTCCCC AAGCTTCAAG TCGGCTTCAT CGACTTTGTG 
TGCACTTTTG TCTATAAGGA GTTCTCCCGA TTTCATGAGG AGATTACACC CATGCTGGAT 
GGGATCACTA ACAACCGCAA GGAATGGAAG GCGCTGGCTG ATGAGTACGA AGCCAAGATG 
AAGGCCCTGG AGGAGGAGAA GCAGAAGCAG CAGGCAGCCA AGCAAGCTGC TTCCGGGAAC 
CAGCCAGGAG GGAACCCCAC TCCAGGGGGT GCACCTGCAT CTAAGTCCTG TTGCATCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666198.1
CDS	132..2714
Translation	MGEVTAEEVEKFLDSNIGFAKQYYNFHYRGKVISDLLGAKEAAVDFSNYHDVNSVEESEIIFDLLRDVQENLQAEKCTFNVMKKLCFLLRADRMSLFMYRTRNGIAELATRLFNVHKDAVLEDCLVMPDSEIVFPLDMGVVGHVAHSKKIANVPNTEEDEHFCDFVDNLTEYQTKNILASPIMNGKDVVAIIMAVNKIDEPHFTKRDEEILLKYLNFVNLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPAYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYVAQNGLICNIMNAPAEDFFEFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDDMDETLMESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQGELPDAESYEINKFHFSDLPLTELELVKCGIQMYYELRVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESTQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYEAKMKALEEEKQKQQAAKQAASGNQPGGNPTPGGAPASKSCCIQ

Target ORF information:

RefSeq Version	NM_146086.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146086.3

ORF Insert Sequence:

ATGGGTGAGG TGACAGCAGA GGAGGTAGAA AAGTTCCTGG ATTCAAATAT TGGCTTTGCC 
AAACAGTACT ATAACTTTCA CTACCGGGGG AAGGTCATCT CAGACCTCCT CGGGGCCAAG 
GAGGCAGCCG TGGACTTCAG CAACTACCAC GATGTGAACA GCGTAGAGGA GAGTGAGATC 
ATCTTTGACC TCCTGCGGGA CGTTCAGGAG AACTTACAGG CTGAGAAATG CACATTCAAT 
GTCATGAAGA AGCTCTGCTT CCTCCTGCGG GCTGACCGCA TGAGCCTGTT CATGTACAGG 
ACCCGCAACG GGATCGCCGA GTTGGCCACT AGGCTCTTCA ATGTCCACAA GGATGCCGTC 
CTAGAGGACT GCTTGGTGAT GCCCGACTCC GAGATTGTCT TCCCTCTGGA CATGGGTGTC 
GTGGGCCACG TCGCACACTC CAAAAAGATT GCCAATGTCC CCAACACAGA AGAGGATGAG 
CATTTCTGTG ACTTCGTGGA CAATCTCACA GAATATCAGA CCAAAAACAT CCTGGCTTCC 
CCCATCATGA ATGGGAAGGA TGTGGTAGCC ATAATCATGG CTGTGAATAA AATAGATGAA 
CCCCACTTCA CCAAGAGAGA TGAAGAGATT CTTCTCAAGT ACCTCAACTT TGTGAACCTG 
ATCATGAAGG TATTCCACCT GAGCTACCTG CACAACTGTG AGACTCGTCG CGGCCAGATA 
TTGCTGTGGT CTGGGAGCAA GGTCTTTGAG GAGCTCACGG ATATAGAGAG GCAGTTCCAC 
AAGGCCCTGT ACACGGTCCG GGCTTTCCTC AACTGTGACA GATACTCCGT AGGACTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TTCTGATGGG CGAGGCTCCA 
GCTTACTCTG GTCCCAGGAC TCCAGACGGA AGGGAAATTA ACTTCTACAA GGTCATTGAC 
TACATCCTGC ACGGCAAAGA AGACATCAAA GTCATCCCGA ACCCACCCGC TGACCACTGG 
GCTCTAGTGA GTGGTCTCCC CACTTACGTG GCTCAAAATG GTCTGATCTG CAATATAATG 
AATGCGCCTG CAGAGGACTT TTTTGAATTC CAGAAAGAGC CTCTGGATGA GTCTGGGTGG 
ATGATTAAAA ATGTACTCTC CATGCCCATC GTCAACAAGA AGGAAGAGAT CGTCGGCGTG 
GCCACATTTT ACAACCGCAA AGATGGGAAG CCCTTCGACG ATATGGACGA GACCCTCATG 
GAGTCTTTGA CTCAGTTTCT GGGATGGTCA GTCTTAAACC CTGACACCTA CGAGTCCATG 
AACAAGCTCG AGAACAGGAA GGATATCTTC CAGGACATCG TGAAATATCA CGTGAAGTGT 
GATAACGAAG AAATCCAGAA GATCTTGAAA ACCAGAGAGG TGTACGGCAA AGAGCCGTGG 
GAATGCGAGG AGGAGGAGCT GGCTGAGATC CTGCAAGGAG AACTTCCAGA CGCGGAGTCA 
TACGAAATCA ACAAGTTCCA CTTCAGCGAC CTGCCACTCA CGGAGCTGGA GCTGGTGAAG 
TGCGGCATCC AGATGTACTA CGAGCTCAGA GTGGTGGACA AGTTCCACAT CCCGCAAGAG 
GCCCTGGTGC GCTTCATGTA TTCGCTAAGC AAAGGCTACC GGAGAATCAC TTACCACAAC 
TGGCGGCATG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC AGGAAAGCTG 
AAACGGTACT TCACTGATCT AGAGGCCTTG GCCATGGTCA CTGCTGCCTT CTGTCATGAC 
ATCGACCACA GAGGCACGAA CAACCTCTAC CAGATGAAAT CACAGAACCC CCTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGGCAT CATTTGGAGT TTGGCAAAAC ACTCCTGAGA 
GATGAGAGCC TGAATATCTT CCAGAACCTG AATCGCCGGC AGCATGAGCA CGCGATCCAC 
ATGATGGACA TCGCGATCAT TGCCACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACCATG 
TTCCAGAAGA TTGTGGATCA GTCAAAGACA TATGAGAGTA CCCAGGAGTG GACCCAGTAC 
ATGATGCTGG AGCAGACACG GAAGGAAATT GTGATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAACC CTGGGAGGTA CAGAGCAAGG TGGCTCTGCT GGTGGCTGCT 
GAATTCTGGG AGCAAGGTGA CCTGGAGCGC ACAGTGCTGC AGCAGAATCC CATTCCCATG 
ATGGACAGAA ACAAGGCGGA TGAGCTCCCC AAGCTTCAAG TCGGCTTCAT CGACTTTGTG 
TGCACTTTTG TCTATAAGGA GTTCTCCCGA TTTCATGAGG AGATTACACC CATGCTGGAT 
GGGATCACTA ACAACCGCAA GGAATGGAAG GCGCTGGCTG ATGAGTACGA AGCCAAGATG 
AAGGCCCTGG AGGAGGAGAA GCAGAAGCAG CAGGCAGCCA AGCAAGCTGC TTCCGGGAAC 
CAGCCAGGAG GGAACCCCAC TCCAGGGGGT GCACCTGCAT CTAAGTCCTG TTGCATCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6a ( XM_006525880.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525943.1 Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), transcript variant X1, mRNA.

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CloneID	OMu54548
Clone ID Related Accession (Same CDS sequence)	XM_006525880.3
Accession Version	XM_006525880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTTT TTGATGTGTG GCCAGTTCTG ATGGGCGAGG CTCCAGCTTA CTCTGGTCCC 
AGGACTCCAG ACGGAAGGGA AATTAACTTC TACAAGGTCA TTGACTACAT CCTGCACGGC 
AAAGAAGACA TCAAAGTCAT CCCGAACCCA CCCGCTGACC ACTGGGCTCT AGTGAGTGGT 
CTCCCCACTT ACGTGGCTCA AAATGGTCTG ATCTGCAATA TAATGAATGC GCCTGCAGAG 
GACTTTTTTG AATTCCAGAA AGAGCCTCTG GATGAGTCTG GGTGGATGAT TAAAAATGTA 
CTCTCCATGC CCATCGTCAA CAAGAAGGAA GAGATCGTCG GCGTGGCCAC ATTTTACAAC 
CGCAAAGATG GGAAGCCCTT CGACGATATG GACGAGACCC TCATGGAGTC TTTGACTCAG 
TTTCTGGGAT GGTCAGTCTT AAACCCTGAC ACCTACGAGT CCATGAACAA GCTCGAGAAC 
AGGAAGGATA TCTTCCAGGA CATCGTGAAA TATCACGTGA AGTGTGATAA CGAAGAAATC 
CAGAAGATCT TGAAAACCAG AGAGGTGTAC GGCAAAGAGC CGTGGGAATG CGAGGAGGAG 
GAGCTGGCTG AGATCCTGCA AGGAGAACTT CCAGACGCGG AGTCATACGA AATCAACAAG 
TTCCACTTCA GCGACCTGCC ACTCACGGAG CTGGAGCTGG TGAAGTGCGG CATCCAGATG 
TACTACGAGC TCAGAGTGGT GGACAAGTTC CACATCCCGC AAGAGGCCCT GGTGCGCTTC 
ATGTATTCGC TAAGCAAAGG CTACCGGAGA ATCACTTACC ACAACTGGCG GCATGGCTTC 
AACGTGGGGC AGACCATGTT CTCCTTGCTG GTGACAGGAA AGCTGAAACG GTACTTCACT 
GATCTAGAGG CCTTGGCCAT GGTCACTGCT GCCTTCTGTC ATGACATCGA CCACAGAGGC 
ACGAACAACC TCTACCAGAT GAAATCACAG AACCCCCTGG CCAAGCTCCA TGGGTCCTCC 
ATCTTGGAAA GGCATCATTT GGAGTTTGGC AAAACACTCC TGAGAGATGA GAGCCTGAAT 
ATCTTCCAGA ACCTGAATCG CCGGCAGCAT GAGCACGCGA TCCACATGAT GGACATCGCG 
ATCATTGCCA CAGACCTTGC CTTGTATTTC AAGAAAAGGA CCATGTTCCA GAAGATTGTG 
GATCAGTCAA AGACATATGA GAGTACCCAG GAGTGGACCC AGTACATGAT GCTGGAGCAG 
ACACGGAAGG AAATTGTGAT GGCCATGATG ATGACCGCCT GTGATCTCTC AGCCATCACC 
AAACCCTGGG AGGTACAGAG CAAGGTGGCT CTGCTGGTGG CTGCTGAATT CTGGGAGCAA 
GGTGACCTGG AGCGCACAGT GCTGCAGCAG AATCCCATTC CCATGATGGA CAGAAACAAG 
GCGGATGAGC TCCCCAAGCT TCAAGTCGGC TTCATCGACT TTGTGTGCAC TTTTGTCTAT 
AAGGAGTTCT CCCGATTTCA TGAGGAGATT ACACCCATGC TGGATGGGAT CACTAACAAC 
CGCAAGGAAT GGAAGGCGCT GGCTGATGAG TACGAAGCCA AGATGAAGGC CCTGGAGGAG 
GAGAAGCAGA AGCAGCAGGC AGCCAAGCAA GGTAGGTGGG TGGAGTCTTG GCCAGGTACA 
CTTTCAGCAC GCAGGACATG CGGGCTTGAA CTGTGGTCGC CATTCTGTGA GGAAGGCCCT 
GAGCCTGCTG AAGGTGAAGT ATTGTGGCCA AGCCATGCAG TCAGTATGTG GCAGAATCTG 
GTCCCTAGAG AGAGGTTGAC AGATGCTCTA GGGTCCTGTA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525943.1
CDS	47..1891
Translation	MEFFDVWPVLMGEAPAYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYVAQNGLICNIMNAPAEDFFEFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDDMDETLMESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQGELPDAESYEINKFHFSDLPLTELELVKCGIQMYYELRVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESTQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYEAKMKALEEEKQKQQAAKQGRWVESWPGTLSARRTCGLELWSPFCEEGPEPAEGEVLWPSHAVSMWQNLVPRERLTDALGSCS

Target ORF information:

RefSeq Version	XM_006525880.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525880.3

ORF Insert Sequence:

ATGGAATTTT TTGATGTGTG GCCAGTTCTG ATGGGCGAGG CTCCAGCTTA CTCTGGTCCC 
AGGACTCCAG ACGGAAGGGA AATTAACTTC TACAAGGTCA TTGACTACAT CCTGCACGGC 
AAAGAAGACA TCAAAGTCAT CCCGAACCCA CCCGCTGACC ACTGGGCTCT AGTGAGTGGT 
CTCCCCACTT ACGTGGCTCA AAATGGTCTG ATCTGCAATA TAATGAATGC GCCTGCAGAG 
GACTTTTTTG AATTCCAGAA AGAGCCTCTG GATGAGTCTG GGTGGATGAT TAAAAATGTA 
CTCTCCATGC CCATCGTCAA CAAGAAGGAA GAGATCGTCG GCGTGGCCAC ATTTTACAAC 
CGCAAAGATG GGAAGCCCTT CGACGATATG GACGAGACCC TCATGGAGTC TTTGACTCAG 
TTTCTGGGAT GGTCAGTCTT AAACCCTGAC ACCTACGAGT CCATGAACAA GCTCGAGAAC 
AGGAAGGATA TCTTCCAGGA CATCGTGAAA TATCACGTGA AGTGTGATAA CGAAGAAATC 
CAGAAGATCT TGAAAACCAG AGAGGTGTAC GGCAAAGAGC CGTGGGAATG CGAGGAGGAG 
GAGCTGGCTG AGATCCTGCA AGGAGAACTT CCAGACGCGG AGTCATACGA AATCAACAAG 
TTCCACTTCA GCGACCTGCC ACTCACGGAG CTGGAGCTGG TGAAGTGCGG CATCCAGATG 
TACTACGAGC TCAGAGTGGT GGACAAGTTC CACATCCCGC AAGAGGCCCT GGTGCGCTTC 
ATGTATTCGC TAAGCAAAGG CTACCGGAGA ATCACTTACC ACAACTGGCG GCATGGCTTC 
AACGTGGGGC AGACCATGTT CTCCTTGCTG GTGACAGGAA AGCTGAAACG GTACTTCACT 
GATCTAGAGG CCTTGGCCAT GGTCACTGCT GCCTTCTGTC ATGACATCGA CCACAGAGGC 
ACGAACAACC TCTACCAGAT GAAATCACAG AACCCCCTGG CCAAGCTCCA TGGGTCCTCC 
ATCTTGGAAA GGCATCATTT GGAGTTTGGC AAAACACTCC TGAGAGATGA GAGCCTGAAT 
ATCTTCCAGA ACCTGAATCG CCGGCAGCAT GAGCACGCGA TCCACATGAT GGACATCGCG 
ATCATTGCCA CAGACCTTGC CTTGTATTTC AAGAAAAGGA CCATGTTCCA GAAGATTGTG 
GATCAGTCAA AGACATATGA GAGTACCCAG GAGTGGACCC AGTACATGAT GCTGGAGCAG 
ACACGGAAGG AAATTGTGAT GGCCATGATG ATGACCGCCT GTGATCTCTC AGCCATCACC 
AAACCCTGGG AGGTACAGAG CAAGGTGGCT CTGCTGGTGG CTGCTGAATT CTGGGAGCAA 
GGTGACCTGG AGCGCACAGT GCTGCAGCAG AATCCCATTC CCATGATGGA CAGAAACAAG 
GCGGATGAGC TCCCCAAGCT TCAAGTCGGC TTCATCGACT TTGTGTGCAC TTTTGTCTAT 
AAGGAGTTCT CCCGATTTCA TGAGGAGATT ACACCCATGC TGGATGGGAT CACTAACAAC 
CGCAAGGAAT GGAAGGCGCT GGCTGATGAG TACGAAGCCA AGATGAAGGC CCTGGAGGAG 
GAGAAGCAGA AGCAGCAGGC AGCCAAGCAA GGTAGGTGGG TGGAGTCTTG GCCAGGTACA 
CTTTCAGCAC GCAGGACATG CGGGCTTGAA CTGTGGTCGC CATTCTGTGA GGAAGGCCCT 
GAGCCTGCTG AAGGTGAAGT ATTGTGGCCA AGCCATGCAG TCAGTATGTG GCAGAATCTG 
GTCCCTAGAG AGAGGTTGAC AGATGCTCTA GGGTCCTGTA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6a ( XM_006525880.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525943.2 Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), transcript variant X1, mRNA.

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CloneID	OMu87385
Clone ID Related Accession (Same CDS sequence)	XM_006525880.4
Accession Version	XM_006525880.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTTT TTGATGTGTG GCCAGTTCTG ATGGGCGAGG CTCCAGCTTA CTCTGGTCCC 
AGGACTCCAG ACGGAAGGGA AATTAACTTC TACAAGGTCA TTGACTACAT CCTGCACGGC 
AAAGAAGACA TCAAAGTCAT CCCGAACCCA CCCGCTGACC ACTGGGCTCT AGTGAGTGGT 
CTCCCCACTT ACGTGGCTCA AAATGGTCTG ATCTGCAATA TAATGAATGC GCCTGCAGAG 
GACTTTTTTG AATTCCAGAA AGAGCCTCTG GATGAGTCTG GGTGGATGAT TAAAAATGTA 
CTCTCCATGC CCATCGTCAA CAAGAAGGAA GAGATCGTCG GCGTGGCCAC ATTTTACAAC 
CGCAAAGATG GGAAGCCCTT CGACGATATG GACGAGACCC TCATGGAGTC TTTGACTCAG 
TTTCTGGGAT GGTCAGTCTT AAACCCTGAC ACCTACGAGT CCATGAACAA GCTCGAGAAC 
AGGAAGGATA TCTTCCAGGA CATCGTGAAA TATCACGTGA AGTGTGATAA CGAAGAAATC 
CAGAAGATCT TGAAAACCAG AGAGGTGTAC GGCAAAGAGC CGTGGGAATG CGAGGAGGAG 
GAGCTGGCTG AGATCCTGCA AGGAGAACTT CCAGACGCGG AGTCATACGA AATCAACAAG 
TTCCACTTCA GCGACCTGCC ACTCACGGAG CTGGAGCTGG TGAAGTGCGG CATCCAGATG 
TACTACGAGC TCAGAGTGGT GGACAAGTTC CACATCCCGC AAGAGGCCCT GGTGCGCTTC 
ATGTATTCGC TAAGCAAAGG CTACCGGAGA ATCACTTACC ACAACTGGCG GCATGGCTTC 
AACGTGGGGC AGACCATGTT CTCCTTGCTG GTGACAGGAA AGCTGAAACG GTACTTCACT 
GATCTAGAGG CCTTGGCCAT GGTCACTGCT GCCTTCTGTC ATGACATCGA CCACAGAGGC 
ACGAACAACC TCTACCAGAT GAAATCACAG AACCCCCTGG CCAAGCTCCA TGGGTCCTCC 
ATCTTGGAAA GGCATCATTT GGAGTTTGGC AAAACACTCC TGAGAGATGA GAGCCTGAAT 
ATCTTCCAGA ACCTGAATCG CCGGCAGCAT GAGCACGCGA TCCACATGAT GGACATCGCG 
ATCATTGCCA CAGACCTTGC CTTGTATTTC AAGAAAAGGA CCATGTTCCA GAAGATTGTG 
GATCAGTCAA AGACATATGA GAGTACCCAG GAGTGGACCC AGTACATGAT GCTGGAGCAG 
ACACGGAAGG AAATTGTGAT GGCCATGATG ATGACCGCCT GTGATCTCTC AGCCATCACC 
AAACCCTGGG AGGTACAGAG CAAGGTGGCT CTGCTGGTGG CTGCTGAATT CTGGGAGCAA 
GGTGACCTGG AGCGCACAGT GCTGCAGCAG AATCCCATTC CCATGATGGA CAGAAACAAG 
GCGGATGAGC TCCCCAAGCT TCAAGTCGGC TTCATCGACT TTGTGTGCAC TTTTGTCTAT 
AAGGAGTTCT CCCGATTTCA TGAGGAGATT ACACCCATGC TGGATGGGAT CACTAACAAC 
CGCAAGGAAT GGAAGGCGCT GGCTGATGAG TACGAAGCCA AGATGAAGGC CCTGGAGGAG 
GAGAAGCAGA AGCAGCAGGC AGCCAAGCAA GCTGCTTCCG GGAACCAGCC AGGAGGGAAC 
CCCACTCCAG GGGGTGCACC TGCATCTAAG TCCTGTTGCA TCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525943.2
CDS	47..1774
Translation	MEFFDVWPVLMGEAPAYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYVAQNGLICNIMNAPAEDFFEFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDDMDETLMESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQGELPDAESYEINKFHFSDLPLTELELVKCGIQMYYELRVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESTQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYEAKMKALEEEKQKQQAAKQAASGNQPGGNPTPGGAPASKSCCIQ

Target ORF information:

RefSeq Version	XM_006525880.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525880.4

ORF Insert Sequence:

ATGGAATTTT TTGATGTGTG GCCAGTTCTG ATGGGCGAGG CTCCAGCTTA CTCTGGTCCC 
AGGACTCCAG ACGGAAGGGA AATTAACTTC TACAAGGTCA TTGACTACAT CCTGCACGGC 
AAAGAAGACA TCAAAGTCAT CCCGAACCCA CCCGCTGACC ACTGGGCTCT AGTGAGTGGT 
CTCCCCACTT ACGTGGCTCA AAATGGTCTG ATCTGCAATA TAATGAATGC GCCTGCAGAG 
GACTTTTTTG AATTCCAGAA AGAGCCTCTG GATGAGTCTG GGTGGATGAT TAAAAATGTA 
CTCTCCATGC CCATCGTCAA CAAGAAGGAA GAGATCGTCG GCGTGGCCAC ATTTTACAAC 
CGCAAAGATG GGAAGCCCTT CGACGATATG GACGAGACCC TCATGGAGTC TTTGACTCAG 
TTTCTGGGAT GGTCAGTCTT AAACCCTGAC ACCTACGAGT CCATGAACAA GCTCGAGAAC 
AGGAAGGATA TCTTCCAGGA CATCGTGAAA TATCACGTGA AGTGTGATAA CGAAGAAATC 
CAGAAGATCT TGAAAACCAG AGAGGTGTAC GGCAAAGAGC CGTGGGAATG CGAGGAGGAG 
GAGCTGGCTG AGATCCTGCA AGGAGAACTT CCAGACGCGG AGTCATACGA AATCAACAAG 
TTCCACTTCA GCGACCTGCC ACTCACGGAG CTGGAGCTGG TGAAGTGCGG CATCCAGATG 
TACTACGAGC TCAGAGTGGT GGACAAGTTC CACATCCCGC AAGAGGCCCT GGTGCGCTTC 
ATGTATTCGC TAAGCAAAGG CTACCGGAGA ATCACTTACC ACAACTGGCG GCATGGCTTC 
AACGTGGGGC AGACCATGTT CTCCTTGCTG GTGACAGGAA AGCTGAAACG GTACTTCACT 
GATCTAGAGG CCTTGGCCAT GGTCACTGCT GCCTTCTGTC ATGACATCGA CCACAGAGGC 
ACGAACAACC TCTACCAGAT GAAATCACAG AACCCCCTGG CCAAGCTCCA TGGGTCCTCC 
ATCTTGGAAA GGCATCATTT GGAGTTTGGC AAAACACTCC TGAGAGATGA GAGCCTGAAT 
ATCTTCCAGA ACCTGAATCG CCGGCAGCAT GAGCACGCGA TCCACATGAT GGACATCGCG 
ATCATTGCCA CAGACCTTGC CTTGTATTTC AAGAAAAGGA CCATGTTCCA GAAGATTGTG 
GATCAGTCAA AGACATATGA GAGTACCCAG GAGTGGACCC AGTACATGAT GCTGGAGCAG 
ACACGGAAGG AAATTGTGAT GGCCATGATG ATGACCGCCT GTGATCTCTC AGCCATCACC 
AAACCCTGGG AGGTACAGAG CAAGGTGGCT CTGCTGGTGG CTGCTGAATT CTGGGAGCAA 
GGTGACCTGG AGCGCACAGT GCTGCAGCAG AATCCCATTC CCATGATGGA CAGAAACAAG 
GCGGATGAGC TCCCCAAGCT TCAAGTCGGC TTCATCGACT TTGTGTGCAC TTTTGTCTAT 
AAGGAGTTCT CCCGATTTCA TGAGGAGATT ACACCCATGC TGGATGGGAT CACTAACAAC 
CGCAAGGAAT GGAAGGCGCT GGCTGATGAG TACGAAGCCA AGATGAAGGC CCTGGAGGAG 
GAGAAGCAGA AGCAGCAGGC AGCCAAGCAA GCTGCTTCCG GGAACCAGCC AGGAGGGAAC 
CCCACTCCAG GGGGTGCACC TGCATCTAAG TCCTGTTGCA TCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ciliopathy-associated IQCB1/NPHP5 protein is required for mouse photoreceptor outer segment formation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(10)3400-3412(2016 10)
Ronquillo CC,Hanke-Gogokhia C,Revelo MP,Frederick JM,Jiang L,Baehr W

Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.
The Journal of biological chemistry291(20)10501-14(2016 May)
Wong BH,Chan JP,Cazenave-Gassiot A,Poh RW,Foo JC,Galam DL,Ghosh S,Nguyen LN,Barathi VA,Yeo SW,Luu CD,Wenk MR,Silver DL

Efficacy of PARP inhibition in Pde6a mutant mouse models for retinitis pigmentosa depends on the quality and composition of individual human mutations.
Cell death discovery216040(2016)
Jiao K,Sahaboglu A,Zrenner E,Ueffing M,Ekstr?m PA,Paquet-Durand F

Retinitis pigmentosa: impact of different Pde6a point mutations on the disease phenotype.
Human molecular genetics24(19)5486-99(2015 Oct)
Sothilingam V,Garcia Garrido M,Jiao K,Buena-Atienza E,Sahaboglu A,Trifunovi? D,Balendran S,Koepfli T,M?hlfriedel R,Sch?n C,Biel M,Heckmann A,Beck SC,Michalakis S,Wissinger B,Seeliger MW,Paquet-Durand F

Alternative Splicing Shapes the Phenotype of a Mutation in BBS8 To Cause Nonsyndromic Retinitis Pigmentosa.
Molecular and cellular biology35(10)1860-70(2015 May)
Murphy D,Singh R,Kolandaivelu S,Ramamurthy V,Stoilov P

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Pde6a cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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