Ucn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ucn
Entrez Gene ID	22226
Full Name	urocortin
Synonyms	Mpv17,Ucn1
General protein information	Preferred Names urocortin Names urocortin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 B1\|5 17.11 cM
Summary	This gene encodes a member of the corticotropin-releasing hormone peptide family that participates in coordinating autonomic, endocrine, and behavioral responses to stress. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional hormone. Mice lacking the encoded protein exhibit a heightened anxiety-like behaviors and a significantly reduced acoustic startle response. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021290.2	NP_067265.1	urocortin preproprotein
NM_001346010.1	NP_001332939.1	urocortin preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UCN	NP_001027472.1
Mus musculus (house mouse)	Ucn	NP_067265.1
Pan troglodytes (chimpanzee)	UCN	XP_001156027.1
Rattus norvegicus (Norway rat)	Ucn	NP_062023.1
Canis lupus familiaris (dog)	UCN	XP_853760.1
Homo sapiens (human)	UCN	NP_003344.1
	UCN	XP_001092536.1

Gene Ontology
Bibliography

Related articles in PubMed

Control of chronic excessive alcohol drinking by genetic manipulation of the Edinger-Westphal nucleus urocortin-1 neuropeptide system.
Giardino WJ, Rodriguez ED, Smith ML, Ford MM, Galili D, Mitchell SH, Chen A, Ryabinin AE
Translational psychiatry7(1)e1021(2017 01)

Sex- and corticotropin-releasing factor receptor 2- dependent actions of urocortin 1 during inflammation.
Hasdemir B, Mhaske P, Paruthiyil S, Garnett EA, Heyman MB, Matloubian M, Bhargava A
American journal of physiology. Regulatory, integrative and comparative physiology310(11)R1244-57(2016 Jun)

Expression of corticotropin-releasing factor and urocortins in the normal and Schistosoma mansoni-infected mouse ileum.
Buckinx R, Bagyanszki M, Avula LR, Adriaensen D, Van Nassauw L, Timmermans JP
Cell and tissue research359(2)453-463(2015 Feb)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Galectin-1 overexpression in endometriosis and its regulation by neuropeptides (CRH, UCN) indicating its important role in reproduction and inflammation.
Vergetaki A, Jeschke U, Vrekoussis T, Taliouri E, Sabatini L, Papakonstanti EA, Makrigiannakis A
PloS one9(12)e114229(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic deletion of Ucn1 reduced high levels of intake and preference across 2 weeks of continuous access drinking when alcohol concentrations were increased from 10 to 20 to 40%. Ucn1 deletion did not alter low-level drinking when the alcohol concentration remained at 10%.
Title: Control of chronic excessive alcohol drinking by genetic manipulation of the Edinger-Westphal nucleus urocortin-1 neuropeptide system.

Taken together, our data indicate that sex shows significant interaction with genotype and Ucn1 during colitis. Human duodenal and colonic biopsies revealed that sex-specific differences exist in levels of CRF receptors and Ucn1 expression in patients with CD compared with the matched non-IBD subjects. To conclude, Ucn1 mediates sex-specific immune and cellular signaling responses via CRF2, emphasizing the need for inclus
Title: Sex- and corticotropin-releasing factor receptor 2- dependent actions of urocortin 1 during inflammation.

This study aims to investigate the cellular localization of CRF and UCNs in the ileum and to explore whether and how this cellular expression is altered in conditions of intestinal Schistosoma mansoni-induced inflammation.
Title: Expression of corticotropin-releasing factor and urocortins in the normal and Schistosoma mansoni-infected mouse ileum.

it is shown that CRH and UCN upregulate galectin-1 expression in Ishikawa cell line and macrophages and this effect is mediated through CRHR1.
Title: Galectin-1 overexpression in endometriosis and its regulation by neuropeptides (CRH, UCN) indicating its important role in reproduction and inflammation.

CNTF activates hypothalamic urocortin 1-expressing neurons both in vitro and in vivo.
Title: The cytokine ciliary neurotrophic factor (CNTF) activates hypothalamic urocortin-expressing neurons both in vitro and in vivo.

The following Ucn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ucn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09809	NM_021290.2 Latest version!	Mus musculus urocortin (Ucn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu09809	NM_001346010.1 Latest version!	Mus musculus urocortin (Ucn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ucn ( NM_021290.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067265.1 Mus musculus urocortin (Ucn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09809
Clone ID Related Accession (Same CDS sequence)	NM_021290.2 , NM_001346010.1
Accession Version	NM_021290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	urocortin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC109608.14. On Dec 1, 2007 this sequence version replaced NM_021290.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC116948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATACAGA GGGGACGCGC TACGCTCCTG GTGGCGTTGC TGCTCTTGGC ACAGCTTCGC 
CCGGAGAGCA GCCAGTGGAG CCCAGCGGCT GCGGCGGCAA CTGGGGTCCA GGATCCGAAT 
CTGCGATGGA GCCCTGGAGT GCGGAATCAG GGCGGCGGCG TCCGCGCGCT CCTCTTGCTG 
TTAGCGGAGC GCTTCCCGCG CCGCGCAGGA TCTGAGCCTG CGGGCGAGCG GCAGCGACGG 
GACGACCCTC CACTGTCCAT CGACCTCACC TTCCACCTGC TGCGGACCCT GCTGGAGCTA 
GCTCGGACAC AGAGCCAGCG CGAGCGCGCA GAGCAGAACC GCATCATATT CGATTCGGTG 
GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067265.1
CDS	113..481
Translation	MIQRGRATLLVALLLLAQLRPESSQWSPAAAAATGVQDPNLRWSPGVRNQGGGVRALLLLLAERFPRRAGSEPAGERQRRDDPPLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSVGK

Target ORF information:

RefSeq Version	NM_021290.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus urocortin (Ucn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021290.2

ORF Insert Sequence:

ATGATACAGA GGGGACGCGC TACGCTCCTG GTGGCGTTGC TGCTCTTGGC ACAGCTTCGC 
CCGGAGAGCA GCCAGTGGAG CCCAGCGGCT GCGGCGGCAA CTGGGGTCCA GGATCCGAAT 
CTGCGATGGA GCCCTGGAGT GCGGAATCAG GGCGGCGGCG TCCGCGCGCT CCTCTTGCTG 
TTAGCGGAGC GCTTCCCGCG CCGCGCAGGA TCTGAGCCTG CGGGCGAGCG GCAGCGACGG 
GACGACCCTC CACTGTCCAT CGACCTCACC TTCCACCTGC TGCGGACCCT GCTGGAGCTA 
GCTCGGACAC AGAGCCAGCG CGAGCGCGCA GAGCAGAACC GCATCATATT CGATTCGGTG 
GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ucn ( NM_001346010.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001332939.1 Mus musculus urocortin (Ucn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09809
Clone ID Related Accession (Same CDS sequence)	NM_021290.2 , NM_001346010.1
Accession Version	NM_001346010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	urocortin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC109608.14 and BC144891.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144891.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATACAGA GGGGACGCGC TACGCTCCTG GTGGCGTTGC TGCTCTTGGC ACAGCTTCGC 
CCGGAGAGCA GCCAGTGGAG CCCAGCGGCT GCGGCGGCAA CTGGGGTCCA GGATCCGAAT 
CTGCGATGGA GCCCTGGAGT GCGGAATCAG GGCGGCGGCG TCCGCGCGCT CCTCTTGCTG 
TTAGCGGAGC GCTTCCCGCG CCGCGCAGGA TCTGAGCCTG CGGGCGAGCG GCAGCGACGG 
GACGACCCTC CACTGTCCAT CGACCTCACC TTCCACCTGC TGCGGACCCT GCTGGAGCTA 
GCTCGGACAC AGAGCCAGCG CGAGCGCGCA GAGCAGAACC GCATCATATT CGATTCGGTG 
GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001332939.1
CDS	108..476
Translation	MIQRGRATLLVALLLLAQLRPESSQWSPAAAAATGVQDPNLRWSPGVRNQGGGVRALLLLLAERFPRRAGSEPAGERQRRDDPPLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSVGK

Target ORF information:

RefSeq Version	NM_001346010.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus urocortin (Ucn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346010.1

ORF Insert Sequence:

ATGATACAGA GGGGACGCGC TACGCTCCTG GTGGCGTTGC TGCTCTTGGC ACAGCTTCGC 
CCGGAGAGCA GCCAGTGGAG CCCAGCGGCT GCGGCGGCAA CTGGGGTCCA GGATCCGAAT 
CTGCGATGGA GCCCTGGAGT GCGGAATCAG GGCGGCGGCG TCCGCGCGCT CCTCTTGCTG 
TTAGCGGAGC GCTTCCCGCG CCGCGCAGGA TCTGAGCCTG CGGGCGAGCG GCAGCGACGG 
GACGACCCTC CACTGTCCAT CGACCTCACC TTCCACCTGC TGCGGACCCT GCTGGAGCTA 
GCTCGGACAC AGAGCCAGCG CGAGCGCGCA GAGCAGAACC GCATCATATT CGATTCGGTG 
GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Control of chronic excessive alcohol drinking by genetic manipulation of the Edinger-Westphal nucleus urocortin-1 neuropeptide system.
Translational psychiatry7(1)e1021(2017 01)
Giardino WJ,Rodriguez ED,Smith ML,Ford MM,Galili D,Mitchell SH,Chen A,Ryabinin AE

Sex- and corticotropin-releasing factor receptor 2- dependent actions of urocortin 1 during inflammation.
American journal of physiology. Regulatory, integrative and comparative physiology310(11)R1244-57(2016 Jun)
Hasdemir B,Mhaske P,Paruthiyil S,Garnett EA,Heyman MB,Matloubian M,Bhargava A

Expression of corticotropin-releasing factor and urocortins in the normal and Schistosoma mansoni-infected mouse ileum.
Cell and tissue research359(2)453-463(2015 Feb)
Buckinx R,Bagyanszki M,Avula LR,Adriaensen D,Van Nassauw L,Timmermans JP

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Galectin-1 overexpression in endometriosis and its regulation by neuropeptides (CRH, UCN) indicating its important role in reproduction and inflammation.
PloS one9(12)e114229(2014)
Vergetaki A,Jeschke U,Vrekoussis T,Taliouri E,Sabatini L,Papakonstanti EA,Makrigiannakis A