Tsc2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017317386.1	XP_017172875.1	tuberin isoform X3
XM_017317387.1	XP_017172876.1	tuberin isoform X4
XM_017317390.1	XP_017172879.1	tuberin isoform X8
XM_017317392.1	XP_017172881.1	tuberin isoform X10
XM_017317384.2	XP_017172873.1	tuberin isoform X12
NM_001286713.1	NP_001273642.1	tuberin isoform 3
XM_017317389.1	XP_017172878.1	tuberin isoform X7
XM_017317391.1	XP_017172880.1	tuberin isoform X8
XM_017317387.2	XP_017172876.1	tuberin isoform X3
XM_017317391.2	XP_017172880.1	tuberin isoform X7
XM_030249648.1	XP_030105508.1	tuberin isoform X7
XM_030249650.1	XP_030105510.1	tuberin isoform X8
NM_001286720.1	NP_001273649.1	tuberin isoform 5
NM_001039363.2	NP_001034452.1	tuberin isoform 2
XM_017317385.1	XP_017172874.1	tuberin isoform X2
XM_006524040.2	XP_006524103.1	tuberin isoform X5
XM_017317388.1	XP_017172877.1	tuberin isoform X6
XM_006524044.2	XP_006524107.1	tuberin isoform X9
XM_017317385.2	XP_017172874.1	tuberin isoform X1
XM_006524040.3	XP_006524103.1	tuberin isoform X4
XM_017317392.2	XP_017172881.1	tuberin isoform X10
NM_001286714.1	NP_001273643.1	tuberin isoform 1
NM_001286718.1	NP_001273647.1	tuberin isoform 4
NM_001286716.1	NP_001273645.1	tuberin isoform 2
XM_017317384.1	XP_017172873.1	tuberin isoform X1
XM_017317388.2	XP_017172877.1	tuberin isoform X5
XM_030249651.1	XP_030105511.1	tuberin isoform X8
XM_017317393.2	XP_017172882.1	tuberin isoform X11
XM_017317393.1	XP_017172882.1	tuberin isoform X11
XM_017317386.2	XP_017172875.1	tuberin isoform X2
XM_030249646.1	XP_030105506.1	tuberin isoform X2
XM_017317389.2	XP_017172878.1	tuberin isoform X6
XM_030249647.1	XP_030105507.1	tuberin isoform X7
XM_017317390.2	XP_017172879.1	tuberin isoform X7
XM_006524044.3	XP_006524107.1	tuberin isoform X9
NM_011647.3	NP_035777.2	tuberin isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23209	NM_001039363.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00
OMu23209	NM_001286716.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00
OMu21891	XM_017317384.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53425	XM_006524040.3 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53428	XM_006524044.3 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79545	XM_017317385.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79546	XM_030249646.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79546	XM_017317386.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79547	XM_017317387.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79548	XM_017317388.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79549	XM_017317389.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79550	XM_017317390.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79550	XM_030249648.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79550	XM_030249647.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79550	XM_017317391.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79551	XM_017317392.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79552	XM_017317393.2 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu87150	XM_030249650.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu87150	XM_030249651.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu18371	NM_001286720.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu21891	NM_001286713.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu23154	NM_011647.3 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu23154	NM_001286714.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu23173	NM_001286718.1 Latest version!	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu79547	XM_017317387.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79550	XM_017317391.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79545	XM_017317385.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53425	XM_006524040.2	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu79551	XM_017317392.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu21891	XM_017317384.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu79548	XM_017317388.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79552	XM_017317393.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$951.30 $1359.00
OMu79546	XM_017317386.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79549	XM_017317389.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79550	XM_017317390.1	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53428	XM_006524044.2	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

Tsc2 ( XM_017317393.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172882.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu79552
Clone ID Related Accession (Same CDS sequence)	XM_017317393.2 , XM_017317393.1
Accession Version	XM_017317393.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG CAGAATACAG ACATCCCTTA CGAGCGCCAG CCTGGGGTCT 
GCGGATGAGA ACTCTATGGC CCAGGCTGAT GACAACTTGA AGAATCTCCA CTTGGAGCTC 
ACAGAAACAT GTCTGGACAT GATGGCCCGA TATGTGTTCT CCAATTTCAC TGCAGTCCCC 
AAGAGGTCCC CTGTGGGAGA GTTCCTCCTG GCAGGGGGTA GGACCAAAAC CTGGCTGGTT 
GGAAACAAGC TTGTCACTGT GACAACAAGT GTGGGAACTG GTACACGGTC GCTGCTGGGC 
CTGGACTCTG GGGACCTGCA GGGTGGCTCG GATTCAAGCT CTGATCCTAG CACACATGTG 
AGGCAGACAA AGGAAGCACC GGCCAAACTG GAGTCCCAGG CTGGGCAGCA GGTGTCCCGT 
GGGGCCCGGG ACCGGGTCCG CTCCATGTCA GGGGGCCATG GCCTTCGAGT TGGTGTCCTG 
GATACTTCAG CTCCCTATTC CCCAGGTGGC TCTGCTTCTC TGGGACCACA GACGGCGGTG 
GCAGCGAAGC CCGAGAAGCC CCCTGCAGGA GCCCAGCTTC CAACAGCAGA GAAGACGAAT 
CTGGCAGCCT ATGTGCCTTT GTTAACCCAG GGCTGGGCAG AAATCTTAGT CCGCAGACCC 
ACAGGAAACA CCAGCTGGCT GATGAGCTTG GAGAACCCGC TCAGCCCCTT CTCCTCAGAC 
ATCAACAACA TGCCCCTGCA AGAGCTGTCC AATGCCCTTA TGGCTGCTGA ACGTTTCAAG 
GAGCACCGGG ACACGGCCCT GTACAAGTCA TTGTCAGTGC CAGCGGCTGG CACCGCCAAG 
CCTCCTACTC TCCCACGCTC TAATACAGAC TCAGCCATGG TCCTTGAGGA GGGAAGTCCA 
GGAGAGACTC AGGTGCCAGT GGAGCCCCCC GAATTGGAGG ATTTCGAGGC GGCGTTAGGT 
ACAGACAGGC ACTGCCAGCG GCCTGACACC TACAGCCGGT CATCCTCAGC ATCTAGCCAG 
GAAGAAAAGT CCCATTTGGA GGAGCTTGCT GCAGGGGGTA TCCCCATTGA GCGGGCCATC 
TCCTCTGAGG GGGCTCGGCC TGCCGTGGAC CTCTCCTTCC AACCCTCACA GCCTTTGAGC 
AAGTCTAGCT CTTCTCCGGA GTTGCAGACC TTACAGGACA TCCTTGGAGA CCTAGGGGAC 
AAGATTGATA TTGGACGGCT GAGTCCTGAG GCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG CAGCTACCTG GTCAGCTACG GGTGAAGAGA GCCGTATCAC AGTCCCACCT 
GAAGGTCCTC TGCCTTCCAG TTCTCCCCGC TCCCCCAGTG GCCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCCGCTCC ATCACGAAGG GGAAAGAGGG TAGAAAGGGA TAACTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAG GTGCCAGGCA TCAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCATTCGCC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTATTGCCC 
AATGAGTCCT TTGAACGGTC AGTACAGCTG CTTGACCAGA TTCCATCCTA TGACACTCAC 
AAGATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAGCA GTGAGCTGGC CATCCTGTCA 
AATGAGCATG GCTCTTACAG GTACACGGAG TTTCTGACAG GCCTGGGTCG GCTTATTGAG 
CTCAAGGACT GCCAACCAGA CAAGGTGTAC TTAGGTGGAC TGGATGTATG TGGCGAGGAT 
GGACAGTTCA CCTACTGCTG GCATGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GACTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAACTGGG CACCATTAAG 
GGCCAGTTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAGTG CAACCTATTG 
ACCCTGCAGT GCAGGAAAGA CATGGAAGGC CTCGTGGACA CCAGTGTGGC CAAGATTGTG 
TCTGACCGCA ACCTGTCCTT TGTGGCCCGA CAGATGGCCC TGCATGCAAA TATGGCCTCA 
CAGGTACACC ACAGCCGATC CAACCCCACT GACATCTATC CCTCCAAGTG GATCGCAAGA 
CTCCGCCACA TTAAGCGTCT CCGCCAGAGG ATCCGTGAAG AGGTGCACTA TTCCAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGCTCAAGC CCCTGAGGCT 
ACACCCACCT ATGAAACAGG CCAGCGGAAG CGCCTCATTT CCTCCGTGGA TGACTTCACA 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172882.1
CDS	229..5280
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317393.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317393.1

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG CAGAATACAG ACATCCCTTA CGAGCGCCAG CCTGGGGTCT 
GCGGATGAGA ACTCTATGGC CCAGGCTGAT GACAACTTGA AGAATCTCCA CTTGGAGCTC 
ACAGAAACAT GTCTGGACAT GATGGCCCGA TATGTGTTCT CCAATTTCAC TGCAGTCCCC 
AAGAGGTCCC CTGTGGGAGA GTTCCTCCTG GCAGGGGGTA GGACCAAAAC CTGGCTGGTT 
GGAAACAAGC TTGTCACTGT GACAACAAGT GTGGGAACTG GTACACGGTC GCTGCTGGGC 
CTGGACTCTG GGGACCTGCA GGGTGGCTCG GATTCAAGCT CTGATCCTAG CACACATGTG 
AGGCAGACAA AGGAAGCACC GGCCAAACTG GAGTCCCAGG CTGGGCAGCA GGTGTCCCGT 
GGGGCCCGGG ACCGGGTCCG CTCCATGTCA GGGGGCCATG GCCTTCGAGT TGGTGTCCTG 
GATACTTCAG CTCCCTATTC CCCAGGTGGC TCTGCTTCTC TGGGACCACA GACGGCGGTG 
GCAGCGAAGC CCGAGAAGCC CCCTGCAGGA GCCCAGCTTC CAACAGCAGA GAAGACGAAT 
CTGGCAGCCT ATGTGCCTTT GTTAACCCAG GGCTGGGCAG AAATCTTAGT CCGCAGACCC 
ACAGGAAACA CCAGCTGGCT GATGAGCTTG GAGAACCCGC TCAGCCCCTT CTCCTCAGAC 
ATCAACAACA TGCCCCTGCA AGAGCTGTCC AATGCCCTTA TGGCTGCTGA ACGTTTCAAG 
GAGCACCGGG ACACGGCCCT GTACAAGTCA TTGTCAGTGC CAGCGGCTGG CACCGCCAAG 
CCTCCTACTC TCCCACGCTC TAATACAGAC TCAGCCATGG TCCTTGAGGA GGGAAGTCCA 
GGAGAGACTC AGGTGCCAGT GGAGCCCCCC GAATTGGAGG ATTTCGAGGC GGCGTTAGGT 
ACAGACAGGC ACTGCCAGCG GCCTGACACC TACAGCCGGT CATCCTCAGC ATCTAGCCAG 
GAAGAAAAGT CCCATTTGGA GGAGCTTGCT GCAGGGGGTA TCCCCATTGA GCGGGCCATC 
TCCTCTGAGG GGGCTCGGCC TGCCGTGGAC CTCTCCTTCC AACCCTCACA GCCTTTGAGC 
AAGTCTAGCT CTTCTCCGGA GTTGCAGACC TTACAGGACA TCCTTGGAGA CCTAGGGGAC 
AAGATTGATA TTGGACGGCT GAGTCCTGAG GCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG CAGCTACCTG GTCAGCTACG GGTGAAGAGA GCCGTATCAC AGTCCCACCT 
GAAGGTCCTC TGCCTTCCAG TTCTCCCCGC TCCCCCAGTG GCCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCCGCTCC ATCACGAAGG GGAAAGAGGG TAGAAAGGGA TAACTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAG GTGCCAGGCA TCAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCATTCGCC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTATTGCCC 
AATGAGTCCT TTGAACGGTC AGTACAGCTG CTTGACCAGA TTCCATCCTA TGACACTCAC 
AAGATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAGCA GTGAGCTGGC CATCCTGTCA 
AATGAGCATG GCTCTTACAG GTACACGGAG TTTCTGACAG GCCTGGGTCG GCTTATTGAG 
CTCAAGGACT GCCAACCAGA CAAGGTGTAC TTAGGTGGAC TGGATGTATG TGGCGAGGAT 
GGACAGTTCA CCTACTGCTG GCATGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GACTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAACTGGG CACCATTAAG 
GGCCAGTTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAGTG CAACCTATTG 
ACCCTGCAGT GCAGGAAAGA CATGGAAGGC CTCGTGGACA CCAGTGTGGC CAAGATTGTG 
TCTGACCGCA ACCTGTCCTT TGTGGCCCGA CAGATGGCCC TGCATGCAAA TATGGCCTCA 
CAGGTACACC ACAGCCGATC CAACCCCACT GACATCTATC CCTCCAAGTG GATCGCAAGA 
CTCCGCCACA TTAAGCGTCT CCGCCAGAGG ATCCGTGAAG AGGTGCACTA TTCCAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGCTCAAGC CCCTGAGGCT 
ACACCCACCT ATGAAACAGG CCAGCGGAAG CGCCTCATTT CCTCCGTGGA TGACTTCACA 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( NM_001039363.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034452.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 2, mRNA.

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CloneID	OMu23209
Clone ID Related Accession (Same CDS sequence)	NM_001039363.2 , NM_001286716.1
Accession Version	NM_001039363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	tuberin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147908.1, AK147874.1, BC052449.1 and AK147302.1. On Nov 14, 2013 this sequence version replaced NM_001039363.1. Transcript Variant: This variant (2) lacks two in-frame exons in the coding region, compared to variant 3. The resulting isoform (2) lacks two internal segments, compared to isoform 3. Variants 2 and 5 encode the same isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170589.1, AK147874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGACTCA GCCATGGTCC TTGAGGAGGG AAGTCCAGGA 
GAGACTCAGG TGCCAGTGGA GCCCCCCGAA TTGGAGGATT TCGAGGCGGC GTTAGGTACA 
GACAGGCACT GCCAGCGGCC TGACACCTAC AGCCGGTCAT CCTCAGCATC TAGCCAGGAA 
GAAAAGTCCC ATTTGGAGGA GCTTGCTGCA GGGGGTATCC CCATTGAGCG GGCCATCTCC 
TCTGAGGGGG CTCGGCCTGC CGTGGACCTC TCCTTCCAAC CCTCACAGCC TTTGAGCAAG 
TCTAGCTCTT CTCCGGAGTT GCAGACCTTA CAGGACATCC TTGGAGACCT AGGGGACAAG 
ATTGATATTG GACGGCTGAG TCCTGAGGCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGCAG CTACCTGGTC AGCTACGGGT GAAGAGAGCC GTATCACAGT CCCACCTGAA 
GGTCCTCTGC CTTCCAGTTC TCCCCGCTCC CCCAGTGGCC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CCGCTCCATC ACGAAGGGGA AAGAGGGTAG AAAGGGATAA CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAGGTG CCAGGCATCA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ATTCGCCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT ATTGCCCAAT 
GAGTCCTTTG AACGGTCAGT ACAGCTGCTT GACCAGATTC CATCCTATGA CACTCACAAG 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAGCAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCATGGCT CTTACAGGTA CACGGAGTTT CTGACAGGCC TGGGTCGGCT TATTGAGCTC 
AAGGACTGCC AACCAGACAA GGTGTACTTA GGTGGACTGG ATGTATGTGG CGAGGATGGA 
CAGTTCACCT ACTGCTGGCA TGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAC 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AACTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAGTGCAA CCTATTGACC 
CTGCAGTGCA GGAAAGACAT GGAAGGCCTC GTGGACACCA GTGTGGCCAA GATTGTGTCT 
GACCGCAACC TGTCCTTTGT GGCCCGACAG ATGGCCCTGC ATGCAAATAT GGCCTCACAG 
GTACACCACA GCCGATCCAA CCCCACTGAC ATCTATCCCT CCAAGTGGAT CGCAAGACTC 
CGCCACATTA AGCGTCTCCG CCAGAGGATC CGTGAAGAGG TGCACTATTC CAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG CTCAAGCCCC TGAGGCTACA 
CCCACCTATG AAACAGGCCA GCGGAAGCGC CTCATTTCCT CCGTGGATGA CTTCACAGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034452.1
CDS	120..5348
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	NM_001039363.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039363.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGACTCA GCCATGGTCC TTGAGGAGGG AAGTCCAGGA 
GAGACTCAGG TGCCAGTGGA GCCCCCCGAA TTGGAGGATT TCGAGGCGGC GTTAGGTACA 
GACAGGCACT GCCAGCGGCC TGACACCTAC AGCCGGTCAT CCTCAGCATC TAGCCAGGAA 
GAAAAGTCCC ATTTGGAGGA GCTTGCTGCA GGGGGTATCC CCATTGAGCG GGCCATCTCC 
TCTGAGGGGG CTCGGCCTGC CGTGGACCTC TCCTTCCAAC CCTCACAGCC TTTGAGCAAG 
TCTAGCTCTT CTCCGGAGTT GCAGACCTTA CAGGACATCC TTGGAGACCT AGGGGACAAG 
ATTGATATTG GACGGCTGAG TCCTGAGGCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGCAG CTACCTGGTC AGCTACGGGT GAAGAGAGCC GTATCACAGT CCCACCTGAA 
GGTCCTCTGC CTTCCAGTTC TCCCCGCTCC CCCAGTGGCC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CCGCTCCATC ACGAAGGGGA AAGAGGGTAG AAAGGGATAA CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAGGTG CCAGGCATCA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ATTCGCCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT ATTGCCCAAT 
GAGTCCTTTG AACGGTCAGT ACAGCTGCTT GACCAGATTC CATCCTATGA CACTCACAAG 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAGCAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCATGGCT CTTACAGGTA CACGGAGTTT CTGACAGGCC TGGGTCGGCT TATTGAGCTC 
AAGGACTGCC AACCAGACAA GGTGTACTTA GGTGGACTGG ATGTATGTGG CGAGGATGGA 
CAGTTCACCT ACTGCTGGCA TGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAC 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AACTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAGTGCAA CCTATTGACC 
CTGCAGTGCA GGAAAGACAT GGAAGGCCTC GTGGACACCA GTGTGGCCAA GATTGTGTCT 
GACCGCAACC TGTCCTTTGT GGCCCGACAG ATGGCCCTGC ATGCAAATAT GGCCTCACAG 
GTACACCACA GCCGATCCAA CCCCACTGAC ATCTATCCCT CCAAGTGGAT CGCAAGACTC 
CGCCACATTA AGCGTCTCCG CCAGAGGATC CGTGAAGAGG TGCACTATTC CAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG CTCAAGCCCC TGAGGCTACA 
CCCACCTATG AAACAGGCCA GCGGAAGCGC CTCATTTCCT CCGTGGATGA CTTCACAGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( NM_001286716.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273645.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 5, mRNA.

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CloneID	OMu23209
Clone ID Related Accession (Same CDS sequence)	NM_001039363.2 , NM_001286716.1
Accession Version	NM_001286716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	tuberin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147908.1, BC052449.1 and AK147302.1. Transcript Variant: This variant (5) has an alternate splice site in the 5' UTR and lacks two in-frame exons in the coding region, compared to variant 3. The resulting isoform (2) lacks two internal segments, compared to isoform 3. Variants 2 and 5 encode the same isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052449.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGACTCA GCCATGGTCC TTGAGGAGGG AAGTCCAGGA 
GAGACTCAGG TGCCAGTGGA GCCCCCCGAA TTGGAGGATT TCGAGGCGGC GTTAGGTACA 
GACAGGCACT GCCAGCGGCC TGACACCTAC AGCCGGTCAT CCTCAGCATC TAGCCAGGAA 
GAAAAGTCCC ATTTGGAGGA GCTTGCTGCA GGGGGTATCC CCATTGAGCG GGCCATCTCC 
TCTGAGGGGG CTCGGCCTGC CGTGGACCTC TCCTTCCAAC CCTCACAGCC TTTGAGCAAG 
TCTAGCTCTT CTCCGGAGTT GCAGACCTTA CAGGACATCC TTGGAGACCT AGGGGACAAG 
ATTGATATTG GACGGCTGAG TCCTGAGGCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGCAG CTACCTGGTC AGCTACGGGT GAAGAGAGCC GTATCACAGT CCCACCTGAA 
GGTCCTCTGC CTTCCAGTTC TCCCCGCTCC CCCAGTGGCC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CCGCTCCATC ACGAAGGGGA AAGAGGGTAG AAAGGGATAA CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAGGTG CCAGGCATCA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ATTCGCCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT ATTGCCCAAT 
GAGTCCTTTG AACGGTCAGT ACAGCTGCTT GACCAGATTC CATCCTATGA CACTCACAAG 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAGCAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCATGGCT CTTACAGGTA CACGGAGTTT CTGACAGGCC TGGGTCGGCT TATTGAGCTC 
AAGGACTGCC AACCAGACAA GGTGTACTTA GGTGGACTGG ATGTATGTGG CGAGGATGGA 
CAGTTCACCT ACTGCTGGCA TGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAC 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AACTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAGTGCAA CCTATTGACC 
CTGCAGTGCA GGAAAGACAT GGAAGGCCTC GTGGACACCA GTGTGGCCAA GATTGTGTCT 
GACCGCAACC TGTCCTTTGT GGCCCGACAG ATGGCCCTGC ATGCAAATAT GGCCTCACAG 
GTACACCACA GCCGATCCAA CCCCACTGAC ATCTATCCCT CCAAGTGGAT CGCAAGACTC 
CGCCACATTA AGCGTCTCCG CCAGAGGATC CGTGAAGAGG TGCACTATTC CAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG CTCAAGCCCC TGAGGCTACA 
CCCACCTATG AAACAGGCCA GCGGAAGCGC CTCATTTCCT CCGTGGATGA CTTCACAGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273645.1
CDS	115..5343
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	NM_001286716.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286716.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGACTCA GCCATGGTCC TTGAGGAGGG AAGTCCAGGA 
GAGACTCAGG TGCCAGTGGA GCCCCCCGAA TTGGAGGATT TCGAGGCGGC GTTAGGTACA 
GACAGGCACT GCCAGCGGCC TGACACCTAC AGCCGGTCAT CCTCAGCATC TAGCCAGGAA 
GAAAAGTCCC ATTTGGAGGA GCTTGCTGCA GGGGGTATCC CCATTGAGCG GGCCATCTCC 
TCTGAGGGGG CTCGGCCTGC CGTGGACCTC TCCTTCCAAC CCTCACAGCC TTTGAGCAAG 
TCTAGCTCTT CTCCGGAGTT GCAGACCTTA CAGGACATCC TTGGAGACCT AGGGGACAAG 
ATTGATATTG GACGGCTGAG TCCTGAGGCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGCAG CTACCTGGTC AGCTACGGGT GAAGAGAGCC GTATCACAGT CCCACCTGAA 
GGTCCTCTGC CTTCCAGTTC TCCCCGCTCC CCCAGTGGCC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CCGCTCCATC ACGAAGGGGA AAGAGGGTAG AAAGGGATAA CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAGGTG CCAGGCATCA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ATTCGCCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT ATTGCCCAAT 
GAGTCCTTTG AACGGTCAGT ACAGCTGCTT GACCAGATTC CATCCTATGA CACTCACAAG 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAGCAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCATGGCT CTTACAGGTA CACGGAGTTT CTGACAGGCC TGGGTCGGCT TATTGAGCTC 
AAGGACTGCC AACCAGACAA GGTGTACTTA GGTGGACTGG ATGTATGTGG CGAGGATGGA 
CAGTTCACCT ACTGCTGGCA TGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAC 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AACTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAGTGCAA CCTATTGACC 
CTGCAGTGCA GGAAAGACAT GGAAGGCCTC GTGGACACCA GTGTGGCCAA GATTGTGTCT 
GACCGCAACC TGTCCTTTGT GGCCCGACAG ATGGCCCTGC ATGCAAATAT GGCCTCACAG 
GTACACCACA GCCGATCCAA CCCCACTGAC ATCTATCCCT CCAAGTGGAT CGCAAGACTC 
CGCCACATTA AGCGTCTCCG CCAGAGGATC CGTGAAGAGG TGCACTATTC CAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG CTCAAGCCCC TGAGGCTACA 
CCCACCTATG AAACAGGCCA GCGGAAGCGC CTCATTTCCT CCGTGGATGA CTTCACAGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317384.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172873.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X17, mRNA.

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CloneID	OMu21891
Clone ID Related Accession (Same CDS sequence)	XM_017317384.2 , NM_001286713.1 , XM_017317384.1
Accession Version	XM_017317384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172873.1
CDS	121..5547
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317384.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317384.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_006524040.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524103.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.

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CloneID	OMu53425
Clone ID Related Accession (Same CDS sequence)	XM_006524040.2 , XM_006524040.3
Accession Version	XM_006524040.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524103.1
CDS	126..5480
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_006524040.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524040.3

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_006524044.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524107.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X14, mRNA.

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CloneID	OMu53428
Clone ID Related Accession (Same CDS sequence)	XM_006524044.2 , XM_006524044.3
Accession Version	XM_006524044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524044.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGCAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGACTCAGCC ATGGTCCTTG AGGAGGGAAG TCCAGGAGAG 
ACTCAGGTGC CAGTGGAGCC CCCCGAATTG GAGGATTTCG AGGCGGCGTT AGGTACAGAC 
AGGCACTGCC AGCGGCCTGA CACCTACAGC CGGTCATCCT CAGCATCTAG CCAGGAAGAA 
AAGTCCCATT TGGAGGAGCT TGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT 
GAGGGGGCTC GGCCTGCCGT GGACCTCTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT 
AGCTCTTCTC CGGAGTTGCA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGATT 
GATATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGGTCCC AGTCAGGGAT CCTGGATGGG 
GAAGCAGCTA CCTGGTCAGC TACGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT 
CCTCTGCCTT CCAGTTCTCC CCGCTCCCCC AGTGGCCTCC GGCCCCGAGG CTATACCATC 
TCTGATTCCG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGATAACTT CAAGAGCAGA 
GCTGCGGCCT CCAGTGCTGA GAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG 
CTCTACCATT CGCCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTATT GCCCAATGAG 
TCCTTTGAAC GGTCAGTACA GCTGCTTGAC CAGATTCCAT CCTATGACAC TCACAAGATT 
GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT GTCAAATGAG 
CATGGCTCTT ACAGGTACAC GGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTCAAG 
GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TATGTGGCGA GGATGGACAG 
TTCACCTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC CACCCTGATG 
CCCACCAAGG ATGTGGACAA GCACCGCTGT GACAAGAAAC GGCACCTGGG CAATGACTTT 
GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAAC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCATGTGAT CATCACACCG CTGGACTACA AGTGCAACCT ATTGACCCTG 
CAGTGCAGGA AAGACATGGA AGGCCTCGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC 
CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA 
CACCACAGCC GATCCAACCC CACTGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC 
CACATTAAGC GTCTCCGCCA GAGGATCCGT GAAGAGGTGC ACTATTCCAA CCCAAGCTTG 
CCTCTGATGC ACCCTCCAGC CCACACCAAA GCCCCAGCTC AAGCCCCTGA GGCTACACCC 
ACCTATGAAA CAGGCCAGCG GAAGCGCCTC ATTTCCTCCG TGGATGACTT CACAGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524107.1
CDS	126..5351
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_006524044.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524044.3

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGCAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGACTCAGCC ATGGTCCTTG AGGAGGGAAG TCCAGGAGAG 
ACTCAGGTGC CAGTGGAGCC CCCCGAATTG GAGGATTTCG AGGCGGCGTT AGGTACAGAC 
AGGCACTGCC AGCGGCCTGA CACCTACAGC CGGTCATCCT CAGCATCTAG CCAGGAAGAA 
AAGTCCCATT TGGAGGAGCT TGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT 
GAGGGGGCTC GGCCTGCCGT GGACCTCTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT 
AGCTCTTCTC CGGAGTTGCA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGATT 
GATATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGGTCCC AGTCAGGGAT CCTGGATGGG 
GAAGCAGCTA CCTGGTCAGC TACGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT 
CCTCTGCCTT CCAGTTCTCC CCGCTCCCCC AGTGGCCTCC GGCCCCGAGG CTATACCATC 
TCTGATTCCG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGATAACTT CAAGAGCAGA 
GCTGCGGCCT CCAGTGCTGA GAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG 
CTCTACCATT CGCCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTATT GCCCAATGAG 
TCCTTTGAAC GGTCAGTACA GCTGCTTGAC CAGATTCCAT CCTATGACAC TCACAAGATT 
GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT GTCAAATGAG 
CATGGCTCTT ACAGGTACAC GGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTCAAG 
GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TATGTGGCGA GGATGGACAG 
TTCACCTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC CACCCTGATG 
CCCACCAAGG ATGTGGACAA GCACCGCTGT GACAAGAAAC GGCACCTGGG CAATGACTTT 
GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAAC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCATGTGAT CATCACACCG CTGGACTACA AGTGCAACCT ATTGACCCTG 
CAGTGCAGGA AAGACATGGA AGGCCTCGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC 
CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA 
CACCACAGCC GATCCAACCC CACTGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC 
CACATTAAGC GTCTCCGCCA GAGGATCCGT GAAGAGGTGC ACTATTCCAA CCCAAGCTTG 
CCTCTGATGC ACCCTCCAGC CCACACCAAA GCCCCAGCTC AAGCCCCTGA GGCTACACCC 
ACCTATGAAA CAGGCCAGCG GAAGCGCCTC ATTTCCTCCG TGGATGACTT CACAGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317385.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172874.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.

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CloneID	OMu79545
Clone ID Related Accession (Same CDS sequence)	XM_017317385.1 , XM_017317385.2
Accession Version	XM_017317385.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172874.1
CDS	117..5540
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317385.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317385.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317385.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172874.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.

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CloneID	OMu79545
Clone ID Related Accession (Same CDS sequence)	XM_017317385.1 , XM_017317385.2
Accession Version	XM_017317385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172874.1
CDS	169..5592
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317385.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317385.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317386.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172875.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.

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CloneID	OMu79546
Clone ID Related Accession (Same CDS sequence)	XM_017317386.1 , XM_017317386.2 , XM_030249646.1
Accession Version	XM_017317386.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172875.1
CDS	114..5537
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317386.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317386.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317386.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172875.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.

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CloneID	OMu79546
Clone ID Related Accession (Same CDS sequence)	XM_017317386.1 , XM_017317386.2 , XM_030249646.1
Accession Version	XM_017317386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172875.1
CDS	126..5549
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317386.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317386.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_030249646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105506.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.

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CloneID	OMu79546
Clone ID Related Accession (Same CDS sequence)	XM_017317386.1 , XM_017317386.2 , XM_030249646.1
Accession Version	XM_030249646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105506.1
CDS	121..5544
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_030249646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249646.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCAGCCAT GGTCCTTGAG 
GAGGGAAGTC CAGGAGAGAC TCAGGTGCCA GTGGAGCCCC CCGAATTGGA GGATTTCGAG 
GCGGCGTTAG GTACAGACAG GCACTGCCAG CGGCCTGACA CCTACAGCCG GTCATCCTCA 
GCATCTAGCC AGGAAGAAAA GTCCCATTTG GAGGAGCTTG CTGCAGGGGG TATCCCCATT 
GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTGCCGTGG ACCTCTCCTT CCAACCCTCA 
CAGCCTTTGA GCAAGTCTAG CTCTTCTCCG GAGTTGCAGA CCTTACAGGA CATCCTTGGA 
GACCTAGGGG ACAAGATTGA TATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGGTCCCAG 
TCAGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTA CGGGTGAAGA GAGCCGTATC 
ACAGTCCCAC CTGAAGGTCC TCTGCCTTCC AGTTCTCCCC GCTCCCCCAG TGGCCTCCGG 
CCCCGAGGCT ATACCATCTC TGATTCCGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG 
GATAACTTCA AGAGCAGAGC TGCGGCCTCC AGTGCTGAGA AGGTGCCAGG CATCAACCCT 
AGCTTTGTGT TCCTACAGCT CTACCATTCG CCCTTTTTTG GTGATGAGTC CAATAAGCCC 
ATCCTATTGC CCAATGAGTC CTTTGAACGG TCAGTACAGC TGCTTGACCA GATTCCATCC 
TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG 
GCCATCCTGT CAAATGAGCA TGGCTCTTAC AGGTACACGG AGTTTCTGAC AGGCCTGGGT 
CGGCTTATTG AGCTCAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTA 
TGTGGCGAGG ATGGACAGTT CACCTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC 
CACATTGCCA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGCTGTGA CAAGAAACGG 
CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAACTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CATGTGATCA TCACACCGCT GGACTACAAG 
TGCAACCTAT TGACCCTGCA GTGCAGGAAA GACATGGAAG GCCTCGTGGA CACCAGTGTG 
GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA 
AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CTGACATCTA TCCCTCCAAG 
TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGCCAGA GGATCCGTGA AGAGGTGCAC 
TATTCCAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ACACCAAAGC CCCAGCTCAA 
GCCCCTGAGG CTACACCCAC CTATGAAACA GGCCAGCGGA AGCGCCTCAT TTCCTCCGTG 
GATGACTTCA CAGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317387.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172876.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.

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CloneID	OMu79547
Clone ID Related Accession (Same CDS sequence)	XM_017317387.1 , XM_017317387.2
Accession Version	XM_017317387.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172876.1
CDS	117..5471
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317387.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317387.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317387.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172876.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.

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CloneID	OMu79547
Clone ID Related Accession (Same CDS sequence)	XM_017317387.1 , XM_017317387.2
Accession Version	XM_017317387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172876.1
CDS	169..5523
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317387.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317387.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317388.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172877.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.

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CloneID	OMu79548
Clone ID Related Accession (Same CDS sequence)	XM_017317388.1 , XM_017317388.2
Accession Version	XM_017317388.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGTGGCT TCTTTCTCCT CCCTGTACCA GCCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172877.1
CDS	117..5414
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317388.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317388.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGTGGCT TCTTTCTCCT CCCTGTACCA GCCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317388.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172877.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.

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CloneID	OMu79548
Clone ID Related Accession (Same CDS sequence)	XM_017317388.1 , XM_017317388.2
Accession Version	XM_017317388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGTGGCT TCTTTCTCCT CCCTGTACCA GCCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172877.1
CDS	169..5466
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317388.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317388.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGCAG AATACAGACA TCCCTTACGA GCGCCAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AACTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ATTTCACTGC AGTCCCCAAG 
AGGTCCCCTG TGGGAGAGTT CCTCCTGGCA GGGGGTAGGA CCAAAACCTG GCTGGTTGGA 
AACAAGCTTG TCACTGTGAC AACAAGTGTG GGAACTGGTA CACGGTCGCT GCTGGGCCTG 
GACTCTGGGG ACCTGCAGGG TGGCTCGGAT TCAAGCTCTG ATCCTAGCAC ACATGTGAGG 
CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGCCATGGCC TTCGAGTTGG TGTCCTGGAT 
ACTTCAGCTC CCTATTCCCC AGGTGGCTCT GCTTCTCTGG GACCACAGAC GGCGGTGGCA 
GCGAAGCCCG AGAAGCCCCC TGCAGGAGCC CAGCTTCCAA CAGCAGAGAA GACGAATCTG 
GCAGCCTATG TGCCTTTGTT AACCCAGGGC TGGGCAGAAA TCTTAGTCCG CAGACCCACA 
GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCCCTTATGG CTGCTGAACG TTTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC CGCCAAGCCT 
CCTACTCTCC CACGCTCTAA TACAGTGGCT TCTTTCTCCT CCCTGTACCA GCCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317389.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172878.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA.

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CloneID	OMu79549
Clone ID Related Accession (Same CDS sequence)	XM_017317389.1 , XM_017317389.2
Accession Version	XM_017317389.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGTGGCTTCT TTCTCCTCCC TGTACCAGCC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172878.1
CDS	117..5411
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317389.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317389.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGTGGCTTCT TTCTCCTCCC TGTACCAGCC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317389.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172878.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.

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CloneID	OMu79549
Clone ID Related Accession (Same CDS sequence)	XM_017317389.1 , XM_017317389.2
Accession Version	XM_017317389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGTGGCTTCT TTCTCCTCCC TGTACCAGCC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172878.1
CDS	169..5463
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317389.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317389.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGTGGCTTCT TTCTCCTCCC TGTACCAGCC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317390.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172879.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA.

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CloneID	OMu79550
Clone ID Related Accession (Same CDS sequence)	XM_017317390.1 , XM_017317391.1 , XM_017317391.2 , XM_030249648.1 , XM_030249647.1 , XM_017317390.2
Accession Version	XM_017317390.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172879.1
CDS	287..5536
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317390.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317390.1

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317391.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172880.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X9, mRNA.

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CloneID	OMu79550
Clone ID Related Accession (Same CDS sequence)	XM_017317390.1 , XM_017317391.1 , XM_017317391.2 , XM_030249648.1 , XM_030249647.1 , XM_017317390.2
Accession Version	XM_017317391.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172880.1
CDS	229..5478
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317391.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317391.1

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317391.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172880.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X10, mRNA.

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CloneID	OMu79550
Clone ID Related Accession (Same CDS sequence)	XM_017317390.1 , XM_017317391.1 , XM_017317391.2 , XM_030249648.1 , XM_030249647.1 , XM_017317390.2
Accession Version	XM_017317391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172880.1
CDS	243..5492
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317391.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317391.2

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_030249648.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105508.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X11, mRNA.

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CloneID	OMu79550
Clone ID Related Accession (Same CDS sequence)	XM_017317390.1 , XM_017317391.1 , XM_017317391.2 , XM_030249648.1 , XM_030249647.1 , XM_017317390.2
Accession Version	XM_030249648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105508.1
CDS	228..5477
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_030249648.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249648.1

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_030249647.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105507.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X8, mRNA.

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CloneID	OMu79550
Clone ID Related Accession (Same CDS sequence)	XM_017317390.1 , XM_017317391.1 , XM_017317391.2 , XM_030249648.1 , XM_030249647.1 , XM_017317390.2
Accession Version	XM_030249647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105507.1
CDS	301..5550
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_030249647.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249647.1

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317390.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172879.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X9, mRNA.

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CloneID	OMu79550
Clone ID Related Accession (Same CDS sequence)	XM_017317390.1 , XM_017317391.1 , XM_017317391.2 , XM_030249648.1 , XM_030249647.1 , XM_017317390.2
Accession Version	XM_017317390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172879.1
CDS	294..5543
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317390.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317390.2

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC 
TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC 
ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCCCTTAC GAGCGCCAGC 
CTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACTTGAA GAATCTCCAC 
TTGGAGCTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAATTTCACT 
GCAGTCCCCA AGAGGTCCCC TGTGGGAGAG TTCCTCCTGG CAGGGGGTAG GACCAAAACC 
TGGCTGGTTG GAAACAAGCT TGTCACTGTG ACAACAAGTG TGGGAACTGG TACACGGTCG 
CTGCTGGGCC TGGACTCTGG GGACCTGCAG GGTGGCTCGG ATTCAAGCTC TGATCCTAGC 
ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG 
GTGTCCCGTG GGGCCCGGGA CCGGGTCCGC TCCATGTCAG GGGGCCATGG CCTTCGAGTT 
GGTGTCCTGG ATACTTCAGC TCCCTATTCC CCAGGTGGCT CTGCTTCTCT GGGACCACAG 
ACGGCGGTGG CAGCGAAGCC CGAGAAGCCC CCTGCAGGAG CCCAGCTTCC AACAGCAGAG 
AAGACGAATC TGGCAGCCTA TGTGCCTTTG TTAACCCAGG GCTGGGCAGA AATCTTAGTC 
CGCAGACCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTC 
TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCCA ATGCCCTTAT GGCTGCTGAA 
CGTTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC 
ACCGCCAAGC CTCCTACTCT CCCACGCTCT AATACAGTGG CTTCTTTCTC CTCCCTGTAC 
CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC AGCCATGGTC 
CTTGAGGAGG GAAGTCCAGG AGAGACTCAG GTGCCAGTGG AGCCCCCCGA ATTGGAGGAT 
TTCGAGGCGG CGTTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACACCTA CAGCCGGTCA 
TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CATTTGGAGG AGCTTGCTGC AGGGGGTATC 
CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTG CCGTGGACCT CTCCTTCCAA 
CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCGGAGT TGCAGACCTT ACAGGACATC 
CTTGGAGACC TAGGGGACAA GATTGATATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGG 
TCCCAGTCAG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTACGGG TGAAGAGAGC 
CGTATCACAG TCCCACCTGA AGGTCCTCTG CCTTCCAGTT CTCCCCGCTC CCCCAGTGGC 
CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCCGCTCCAT CACGAAGGGG AAAGAGGGTA 
GAAAGGGATA ACTTCAAGAG CAGAGCTGCG GCCTCCAGTG CTGAGAAGGT GCCAGGCATC 
AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCGCCCT TTTTTGGTGA TGAGTCCAAT 
AAGCCCATCC TATTGCCCAA TGAGTCCTTT GAACGGTCAG TACAGCTGCT TGACCAGATT 
CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTGGCCA TCCTGTCAAA TGAGCATGGC TCTTACAGGT ACACGGAGTT TCTGACAGGC 
CTGGGTCGGC TTATTGAGCT CAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG 
GATGTATGTG GCGAGGATGG ACAGTTCACC TACTGCTGGC ATGATGACAT CATGCAAGCT 
GTTTTCCACA TTGCCACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG CTGTGACAAG 
AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC 
AAACTGGGCA CCATTAAGGG CCAGTTCAAC TTTGTCCATG TGATCATCAC ACCGCTGGAC 
TACAAGTGCA ACCTATTGAC CCTGCAGTGC AGGAAAGACA TGGAAGGCCT CGTGGACACC 
AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG 
CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACTGA CATCTATCCC 
TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GCCAGAGGAT CCGTGAAGAG 
GTGCACTATT CCAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCACAC CAAAGCCCCA 
GCTCAAGCCC CTGAGGCTAC ACCCACCTAT GAAACAGGCC AGCGGAAGCG CCTCATTTCC 
TCCGTGGATG ACTTCACAGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317392.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172881.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X11, mRNA.

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CloneID	OMu79551
Clone ID Related Accession (Same CDS sequence)	XM_017317392.1 , XM_017317392.2
Accession Version	XM_017317392.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGAATACA GACATCCCTT ACGAGCGCCA GCCTGGGGTC TGCGGATGAG 
AACTCTATGG CCCAGGCTGA TGACAACTTG AAGAATCTCC ACTTGGAGCT CACAGAAACA 
TGTCTGGACA TGATGGCCCG ATATGTGTTC TCCAATTTCA CTGCAGTCCC CAAGAGGTCC 
CCTGTGGGAG AGTTCCTCCT GGCAGGGGGT AGGACCAAAA CCTGGCTGGT TGGAAACAAG 
CTTGTCACTG TGACAACAAG TGTGGGAACT GGTACACGGT CGCTGCTGGG CCTGGACTCT 
GGGGACCTGC AGGGTGGCTC GGATTCAAGC TCTGATCCTA GCACACATGT GAGGCAGACA 
AAGGAAGCAC CGGCCAAACT GGAGTCCCAG GCTGGGCAGC AGGTGTCCCG TGGGGCCCGG 
GACCGGGTCC GCTCCATGTC AGGGGGCCAT GGCCTTCGAG TTGGTGTCCT GGATACTTCA 
GCTCCCTATT CCCCAGGTGG CTCTGCTTCT CTGGGACCAC AGACGGCGGT GGCAGCGAAG 
CCCGAGAAGC CCCCTGCAGG AGCCCAGCTT CCAACAGCAG AGAAGACGAA TCTGGCAGCC 
TATGTGCCTT TGTTAACCCA GGGCTGGGCA GAAATCTTAG TCCGCAGACC CACAGGAAAC 
ACCAGCTGGC TGATGAGCTT GGAGAACCCG CTCAGCCCCT TCTCCTCAGA CATCAACAAC 
ATGCCCCTGC AAGAGCTGTC CAATGCCCTT ATGGCTGCTG AACGTTTCAA GGAGCACCGG 
GACACGGCCC TGTACAAGTC ATTGTCAGTG CCAGCGGCTG GCACCGCCAA GCCTCCTACT 
CTCCCACGCT CTAATACAGA CTCAGCCATG GTCCTTGAGG AGGGAAGTCC AGGAGAGACT 
CAGGTGCCAG TGGAGCCCCC CGAATTGGAG GATTTCGAGG CGGCGTTAGG TACAGACAGG 
CACTGCCAGC GGCCTGACAC CTACAGCCGG TCATCCTCAG CATCTAGCCA GGAAGAAAAG 
TCCCATTTGG AGGAGCTTGC TGCAGGGGGT ATCCCCATTG AGCGGGCCAT CTCCTCTGAG 
GGGGCTCGGC CTGCCGTGGA CCTCTCCTTC CAACCCTCAC AGCCTTTGAG CAAGTCTAGC 
TCTTCTCCGG AGTTGCAGAC CTTACAGGAC ATCCTTGGAG ACCTAGGGGA CAAGATTGAT 
ATTGGACGGC TGAGTCCTGA GGCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GCAGCTACCT GGTCAGCTAC GGGTGAAGAG AGCCGTATCA CAGTCCCACC TGAAGGTCCT 
CTGCCTTCCA GTTCTCCCCG CTCCCCCAGT GGCCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCCGCTC CATCACGAAG GGGAAAGAGG GTAGAAAGGG ATAACTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA GGTGCCAGGC ATCAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCATTCGC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTATTGCC CAATGAGTCC 
TTTGAACGGT CAGTACAGCT GCTTGACCAG ATTCCATCCT ATGACACTCA CAAGATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAGC AGTGAGCTGG CCATCCTGTC AAATGAGCAT 
GGCTCTTACA GGTACACGGA GTTTCTGACA GGCCTGGGTC GGCTTATTGA GCTCAAGGAC 
TGCCAACCAG ACAAGGTGTA CTTAGGTGGA CTGGATGTAT GTGGCGAGGA TGGACAGTTC 
ACCTACTGCT GGCATGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGACTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAACTGG GCACCATTAA GGGCCAGTTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAGT GCAACCTATT GACCCTGCAG 
TGCAGGAAAG ACATGGAAGG CCTCGTGGAC ACCAGTGTGG CCAAGATTGT GTCTGACCGC 
AACCTGTCCT TTGTGGCCCG ACAGATGGCC CTGCATGCAA ATATGGCCTC ACAGGTACAC 
CACAGCCGAT CCAACCCCAC TGACATCTAT CCCTCCAAGT GGATCGCAAG ACTCCGCCAC 
ATTAAGCGTC TCCGCCAGAG GATCCGTGAA GAGGTGCACT ATTCCAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGCTCAAG CCCCTGAGGC TACACCCACC 
TATGAAACAG GCCAGCGGAA GCGCCTCATT TCCTCCGTGG ATGACTTCAC AGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172881.1
CDS	114..5336
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317392.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317392.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGAATACA GACATCCCTT ACGAGCGCCA GCCTGGGGTC TGCGGATGAG 
AACTCTATGG CCCAGGCTGA TGACAACTTG AAGAATCTCC ACTTGGAGCT CACAGAAACA 
TGTCTGGACA TGATGGCCCG ATATGTGTTC TCCAATTTCA CTGCAGTCCC CAAGAGGTCC 
CCTGTGGGAG AGTTCCTCCT GGCAGGGGGT AGGACCAAAA CCTGGCTGGT TGGAAACAAG 
CTTGTCACTG TGACAACAAG TGTGGGAACT GGTACACGGT CGCTGCTGGG CCTGGACTCT 
GGGGACCTGC AGGGTGGCTC GGATTCAAGC TCTGATCCTA GCACACATGT GAGGCAGACA 
AAGGAAGCAC CGGCCAAACT GGAGTCCCAG GCTGGGCAGC AGGTGTCCCG TGGGGCCCGG 
GACCGGGTCC GCTCCATGTC AGGGGGCCAT GGCCTTCGAG TTGGTGTCCT GGATACTTCA 
GCTCCCTATT CCCCAGGTGG CTCTGCTTCT CTGGGACCAC AGACGGCGGT GGCAGCGAAG 
CCCGAGAAGC CCCCTGCAGG AGCCCAGCTT CCAACAGCAG AGAAGACGAA TCTGGCAGCC 
TATGTGCCTT TGTTAACCCA GGGCTGGGCA GAAATCTTAG TCCGCAGACC CACAGGAAAC 
ACCAGCTGGC TGATGAGCTT GGAGAACCCG CTCAGCCCCT TCTCCTCAGA CATCAACAAC 
ATGCCCCTGC AAGAGCTGTC CAATGCCCTT ATGGCTGCTG AACGTTTCAA GGAGCACCGG 
GACACGGCCC TGTACAAGTC ATTGTCAGTG CCAGCGGCTG GCACCGCCAA GCCTCCTACT 
CTCCCACGCT CTAATACAGA CTCAGCCATG GTCCTTGAGG AGGGAAGTCC AGGAGAGACT 
CAGGTGCCAG TGGAGCCCCC CGAATTGGAG GATTTCGAGG CGGCGTTAGG TACAGACAGG 
CACTGCCAGC GGCCTGACAC CTACAGCCGG TCATCCTCAG CATCTAGCCA GGAAGAAAAG 
TCCCATTTGG AGGAGCTTGC TGCAGGGGGT ATCCCCATTG AGCGGGCCAT CTCCTCTGAG 
GGGGCTCGGC CTGCCGTGGA CCTCTCCTTC CAACCCTCAC AGCCTTTGAG CAAGTCTAGC 
TCTTCTCCGG AGTTGCAGAC CTTACAGGAC ATCCTTGGAG ACCTAGGGGA CAAGATTGAT 
ATTGGACGGC TGAGTCCTGA GGCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GCAGCTACCT GGTCAGCTAC GGGTGAAGAG AGCCGTATCA CAGTCCCACC TGAAGGTCCT 
CTGCCTTCCA GTTCTCCCCG CTCCCCCAGT GGCCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCCGCTC CATCACGAAG GGGAAAGAGG GTAGAAAGGG ATAACTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA GGTGCCAGGC ATCAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCATTCGC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTATTGCC CAATGAGTCC 
TTTGAACGGT CAGTACAGCT GCTTGACCAG ATTCCATCCT ATGACACTCA CAAGATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAGC AGTGAGCTGG CCATCCTGTC AAATGAGCAT 
GGCTCTTACA GGTACACGGA GTTTCTGACA GGCCTGGGTC GGCTTATTGA GCTCAAGGAC 
TGCCAACCAG ACAAGGTGTA CTTAGGTGGA CTGGATGTAT GTGGCGAGGA TGGACAGTTC 
ACCTACTGCT GGCATGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGACTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAACTGG GCACCATTAA GGGCCAGTTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAGT GCAACCTATT GACCCTGCAG 
TGCAGGAAAG ACATGGAAGG CCTCGTGGAC ACCAGTGTGG CCAAGATTGT GTCTGACCGC 
AACCTGTCCT TTGTGGCCCG ACAGATGGCC CTGCATGCAA ATATGGCCTC ACAGGTACAC 
CACAGCCGAT CCAACCCCAC TGACATCTAT CCCTCCAAGT GGATCGCAAG ACTCCGCCAC 
ATTAAGCGTC TCCGCCAGAG GATCCGTGAA GAGGTGCACT ATTCCAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGCTCAAG CCCCTGAGGC TACACCCACC 
TATGAAACAG GCCAGCGGAA GCGCCTCATT TCCTCCGTGG ATGACTTCAC AGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317392.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172881.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X15, mRNA.

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CloneID	OMu79551
Clone ID Related Accession (Same CDS sequence)	XM_017317392.1 , XM_017317392.2
Accession Version	XM_017317392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGAATACA GACATCCCTT ACGAGCGCCA GCCTGGGGTC TGCGGATGAG 
AACTCTATGG CCCAGGCTGA TGACAACTTG AAGAATCTCC ACTTGGAGCT CACAGAAACA 
TGTCTGGACA TGATGGCCCG ATATGTGTTC TCCAATTTCA CTGCAGTCCC CAAGAGGTCC 
CCTGTGGGAG AGTTCCTCCT GGCAGGGGGT AGGACCAAAA CCTGGCTGGT TGGAAACAAG 
CTTGTCACTG TGACAACAAG TGTGGGAACT GGTACACGGT CGCTGCTGGG CCTGGACTCT 
GGGGACCTGC AGGGTGGCTC GGATTCAAGC TCTGATCCTA GCACACATGT GAGGCAGACA 
AAGGAAGCAC CGGCCAAACT GGAGTCCCAG GCTGGGCAGC AGGTGTCCCG TGGGGCCCGG 
GACCGGGTCC GCTCCATGTC AGGGGGCCAT GGCCTTCGAG TTGGTGTCCT GGATACTTCA 
GCTCCCTATT CCCCAGGTGG CTCTGCTTCT CTGGGACCAC AGACGGCGGT GGCAGCGAAG 
CCCGAGAAGC CCCCTGCAGG AGCCCAGCTT CCAACAGCAG AGAAGACGAA TCTGGCAGCC 
TATGTGCCTT TGTTAACCCA GGGCTGGGCA GAAATCTTAG TCCGCAGACC CACAGGAAAC 
ACCAGCTGGC TGATGAGCTT GGAGAACCCG CTCAGCCCCT TCTCCTCAGA CATCAACAAC 
ATGCCCCTGC AAGAGCTGTC CAATGCCCTT ATGGCTGCTG AACGTTTCAA GGAGCACCGG 
GACACGGCCC TGTACAAGTC ATTGTCAGTG CCAGCGGCTG GCACCGCCAA GCCTCCTACT 
CTCCCACGCT CTAATACAGA CTCAGCCATG GTCCTTGAGG AGGGAAGTCC AGGAGAGACT 
CAGGTGCCAG TGGAGCCCCC CGAATTGGAG GATTTCGAGG CGGCGTTAGG TACAGACAGG 
CACTGCCAGC GGCCTGACAC CTACAGCCGG TCATCCTCAG CATCTAGCCA GGAAGAAAAG 
TCCCATTTGG AGGAGCTTGC TGCAGGGGGT ATCCCCATTG AGCGGGCCAT CTCCTCTGAG 
GGGGCTCGGC CTGCCGTGGA CCTCTCCTTC CAACCCTCAC AGCCTTTGAG CAAGTCTAGC 
TCTTCTCCGG AGTTGCAGAC CTTACAGGAC ATCCTTGGAG ACCTAGGGGA CAAGATTGAT 
ATTGGACGGC TGAGTCCTGA GGCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GCAGCTACCT GGTCAGCTAC GGGTGAAGAG AGCCGTATCA CAGTCCCACC TGAAGGTCCT 
CTGCCTTCCA GTTCTCCCCG CTCCCCCAGT GGCCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCCGCTC CATCACGAAG GGGAAAGAGG GTAGAAAGGG ATAACTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA GGTGCCAGGC ATCAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCATTCGC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTATTGCC CAATGAGTCC 
TTTGAACGGT CAGTACAGCT GCTTGACCAG ATTCCATCCT ATGACACTCA CAAGATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAGC AGTGAGCTGG CCATCCTGTC AAATGAGCAT 
GGCTCTTACA GGTACACGGA GTTTCTGACA GGCCTGGGTC GGCTTATTGA GCTCAAGGAC 
TGCCAACCAG ACAAGGTGTA CTTAGGTGGA CTGGATGTAT GTGGCGAGGA TGGACAGTTC 
ACCTACTGCT GGCATGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGACTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAACTGG GCACCATTAA GGGCCAGTTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAGT GCAACCTATT GACCCTGCAG 
TGCAGGAAAG ACATGGAAGG CCTCGTGGAC ACCAGTGTGG CCAAGATTGT GTCTGACCGC 
AACCTGTCCT TTGTGGCCCG ACAGATGGCC CTGCATGCAA ATATGGCCTC ACAGGTACAC 
CACAGCCGAT CCAACCCCAC TGACATCTAT CCCTCCAAGT GGATCGCAAG ACTCCGCCAC 
ATTAAGCGTC TCCGCCAGAG GATCCGTGAA GAGGTGCACT ATTCCAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGCTCAAG CCCCTGAGGC TACACCCACC 
TATGAAACAG GCCAGCGGAA GCGCCTCATT TCCTCCGTGG ATGACTTCAC AGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172881.1
CDS	126..5348
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317392.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317392.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGAATACA GACATCCCTT ACGAGCGCCA GCCTGGGGTC TGCGGATGAG 
AACTCTATGG CCCAGGCTGA TGACAACTTG AAGAATCTCC ACTTGGAGCT CACAGAAACA 
TGTCTGGACA TGATGGCCCG ATATGTGTTC TCCAATTTCA CTGCAGTCCC CAAGAGGTCC 
CCTGTGGGAG AGTTCCTCCT GGCAGGGGGT AGGACCAAAA CCTGGCTGGT TGGAAACAAG 
CTTGTCACTG TGACAACAAG TGTGGGAACT GGTACACGGT CGCTGCTGGG CCTGGACTCT 
GGGGACCTGC AGGGTGGCTC GGATTCAAGC TCTGATCCTA GCACACATGT GAGGCAGACA 
AAGGAAGCAC CGGCCAAACT GGAGTCCCAG GCTGGGCAGC AGGTGTCCCG TGGGGCCCGG 
GACCGGGTCC GCTCCATGTC AGGGGGCCAT GGCCTTCGAG TTGGTGTCCT GGATACTTCA 
GCTCCCTATT CCCCAGGTGG CTCTGCTTCT CTGGGACCAC AGACGGCGGT GGCAGCGAAG 
CCCGAGAAGC CCCCTGCAGG AGCCCAGCTT CCAACAGCAG AGAAGACGAA TCTGGCAGCC 
TATGTGCCTT TGTTAACCCA GGGCTGGGCA GAAATCTTAG TCCGCAGACC CACAGGAAAC 
ACCAGCTGGC TGATGAGCTT GGAGAACCCG CTCAGCCCCT TCTCCTCAGA CATCAACAAC 
ATGCCCCTGC AAGAGCTGTC CAATGCCCTT ATGGCTGCTG AACGTTTCAA GGAGCACCGG 
GACACGGCCC TGTACAAGTC ATTGTCAGTG CCAGCGGCTG GCACCGCCAA GCCTCCTACT 
CTCCCACGCT CTAATACAGA CTCAGCCATG GTCCTTGAGG AGGGAAGTCC AGGAGAGACT 
CAGGTGCCAG TGGAGCCCCC CGAATTGGAG GATTTCGAGG CGGCGTTAGG TACAGACAGG 
CACTGCCAGC GGCCTGACAC CTACAGCCGG TCATCCTCAG CATCTAGCCA GGAAGAAAAG 
TCCCATTTGG AGGAGCTTGC TGCAGGGGGT ATCCCCATTG AGCGGGCCAT CTCCTCTGAG 
GGGGCTCGGC CTGCCGTGGA CCTCTCCTTC CAACCCTCAC AGCCTTTGAG CAAGTCTAGC 
TCTTCTCCGG AGTTGCAGAC CTTACAGGAC ATCCTTGGAG ACCTAGGGGA CAAGATTGAT 
ATTGGACGGC TGAGTCCTGA GGCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GCAGCTACCT GGTCAGCTAC GGGTGAAGAG AGCCGTATCA CAGTCCCACC TGAAGGTCCT 
CTGCCTTCCA GTTCTCCCCG CTCCCCCAGT GGCCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCCGCTC CATCACGAAG GGGAAAGAGG GTAGAAAGGG ATAACTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA GGTGCCAGGC ATCAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCATTCGC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTATTGCC CAATGAGTCC 
TTTGAACGGT CAGTACAGCT GCTTGACCAG ATTCCATCCT ATGACACTCA CAAGATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAGC AGTGAGCTGG CCATCCTGTC AAATGAGCAT 
GGCTCTTACA GGTACACGGA GTTTCTGACA GGCCTGGGTC GGCTTATTGA GCTCAAGGAC 
TGCCAACCAG ACAAGGTGTA CTTAGGTGGA CTGGATGTAT GTGGCGAGGA TGGACAGTTC 
ACCTACTGCT GGCATGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGACTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAACTGG GCACCATTAA GGGCCAGTTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAGT GCAACCTATT GACCCTGCAG 
TGCAGGAAAG ACATGGAAGG CCTCGTGGAC ACCAGTGTGG CCAAGATTGT GTCTGACCGC 
AACCTGTCCT TTGTGGCCCG ACAGATGGCC CTGCATGCAA ATATGGCCTC ACAGGTACAC 
CACAGCCGAT CCAACCCCAC TGACATCTAT CCCTCCAAGT GGATCGCAAG ACTCCGCCAC 
ATTAAGCGTC TCCGCCAGAG GATCCGTGAA GAGGTGCACT ATTCCAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGCTCAAG CCCCTGAGGC TACACCCACC 
TATGAAACAG GCCAGCGGAA GCGCCTCATT TCCTCCGTGG ATGACTTCAC AGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317393.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172882.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X16, mRNA.

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CloneID	OMu79552
Clone ID Related Accession (Same CDS sequence)	XM_017317393.2 , XM_017317393.1
Accession Version	XM_017317393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG CAGAATACAG ACATCCCTTA CGAGCGCCAG CCTGGGGTCT 
GCGGATGAGA ACTCTATGGC CCAGGCTGAT GACAACTTGA AGAATCTCCA CTTGGAGCTC 
ACAGAAACAT GTCTGGACAT GATGGCCCGA TATGTGTTCT CCAATTTCAC TGCAGTCCCC 
AAGAGGTCCC CTGTGGGAGA GTTCCTCCTG GCAGGGGGTA GGACCAAAAC CTGGCTGGTT 
GGAAACAAGC TTGTCACTGT GACAACAAGT GTGGGAACTG GTACACGGTC GCTGCTGGGC 
CTGGACTCTG GGGACCTGCA GGGTGGCTCG GATTCAAGCT CTGATCCTAG CACACATGTG 
AGGCAGACAA AGGAAGCACC GGCCAAACTG GAGTCCCAGG CTGGGCAGCA GGTGTCCCGT 
GGGGCCCGGG ACCGGGTCCG CTCCATGTCA GGGGGCCATG GCCTTCGAGT TGGTGTCCTG 
GATACTTCAG CTCCCTATTC CCCAGGTGGC TCTGCTTCTC TGGGACCACA GACGGCGGTG 
GCAGCGAAGC CCGAGAAGCC CCCTGCAGGA GCCCAGCTTC CAACAGCAGA GAAGACGAAT 
CTGGCAGCCT ATGTGCCTTT GTTAACCCAG GGCTGGGCAG AAATCTTAGT CCGCAGACCC 
ACAGGAAACA CCAGCTGGCT GATGAGCTTG GAGAACCCGC TCAGCCCCTT CTCCTCAGAC 
ATCAACAACA TGCCCCTGCA AGAGCTGTCC AATGCCCTTA TGGCTGCTGA ACGTTTCAAG 
GAGCACCGGG ACACGGCCCT GTACAAGTCA TTGTCAGTGC CAGCGGCTGG CACCGCCAAG 
CCTCCTACTC TCCCACGCTC TAATACAGAC TCAGCCATGG TCCTTGAGGA GGGAAGTCCA 
GGAGAGACTC AGGTGCCAGT GGAGCCCCCC GAATTGGAGG ATTTCGAGGC GGCGTTAGGT 
ACAGACAGGC ACTGCCAGCG GCCTGACACC TACAGCCGGT CATCCTCAGC ATCTAGCCAG 
GAAGAAAAGT CCCATTTGGA GGAGCTTGCT GCAGGGGGTA TCCCCATTGA GCGGGCCATC 
TCCTCTGAGG GGGCTCGGCC TGCCGTGGAC CTCTCCTTCC AACCCTCACA GCCTTTGAGC 
AAGTCTAGCT CTTCTCCGGA GTTGCAGACC TTACAGGACA TCCTTGGAGA CCTAGGGGAC 
AAGATTGATA TTGGACGGCT GAGTCCTGAG GCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG CAGCTACCTG GTCAGCTACG GGTGAAGAGA GCCGTATCAC AGTCCCACCT 
GAAGGTCCTC TGCCTTCCAG TTCTCCCCGC TCCCCCAGTG GCCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCCGCTCC ATCACGAAGG GGAAAGAGGG TAGAAAGGGA TAACTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAG GTGCCAGGCA TCAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCATTCGCC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTATTGCCC 
AATGAGTCCT TTGAACGGTC AGTACAGCTG CTTGACCAGA TTCCATCCTA TGACACTCAC 
AAGATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAGCA GTGAGCTGGC CATCCTGTCA 
AATGAGCATG GCTCTTACAG GTACACGGAG TTTCTGACAG GCCTGGGTCG GCTTATTGAG 
CTCAAGGACT GCCAACCAGA CAAGGTGTAC TTAGGTGGAC TGGATGTATG TGGCGAGGAT 
GGACAGTTCA CCTACTGCTG GCATGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GACTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAACTGGG CACCATTAAG 
GGCCAGTTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAGTG CAACCTATTG 
ACCCTGCAGT GCAGGAAAGA CATGGAAGGC CTCGTGGACA CCAGTGTGGC CAAGATTGTG 
TCTGACCGCA ACCTGTCCTT TGTGGCCCGA CAGATGGCCC TGCATGCAAA TATGGCCTCA 
CAGGTACACC ACAGCCGATC CAACCCCACT GACATCTATC CCTCCAAGTG GATCGCAAGA 
CTCCGCCACA TTAAGCGTCT CCGCCAGAGG ATCCGTGAAG AGGTGCACTA TTCCAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGCTCAAGC CCCTGAGGCT 
ACACCCACCT ATGAAACAGG CCAGCGGAAG CGCCTCATTT CCTCCGTGGA TGACTTCACA 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172882.1
CDS	243..5294
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317393.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317393.2

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA 
CTTGTCAACC TGGTCAAGTT CAACAGCTGT TACCTTGACG AATACATTGC ATCAATGGTT 
CACATGATTT GTCTGCTATG CATCCGGACA GTGTCCTCTG TGGACATTGA GGTGTCCTTG 
CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCTTACCAG CCGAGAGCCT GCCTCTGTTC 
ATTATCACCC TGTGCCGCAC CATCAATGTC AAGGAGCTGT GTGAGCCTTG CTGGAAGTTG 
ATGCGTAACC TTCTGGGCAC CCACCTGGGC CACAGCGCCA TCTACAACAT GTGCCGCATC 
ATGGAGGACA GATCCTACAT GGAAGATGCC CCACTGCTGA GAGGAGCTGT GTTCTTTGTA 
GGGATGGCAC TCTGGGGAGC TCACCGGCTC TACTCTCTCA AGAACTCCCC CACATCTGTG 
CTGCCGTCTT TTTATGAGGC TATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT 
CTGTCCATAA CAAGACTCAT CAAGAAGTAT AGGAAGGAGC TCCAGGCTGT GACATGGGAT 
ATTCTGCTGG ACATCATTGA ACGACTACTT CAGCAACTCC AGAACCTGGA CAGCCCGGAA 
CTCAAGACCA TCGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTATGTGA CCAGAACGAG 
TTCCATGGCT CGCAGGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGACCT 
GAATCCTCTC TCTTAAACTT GATATCCTAC AGAGCCCAGT CCATCCACCC TGCCAAGGAT 
GGCTGGATCC AGAACTTGCA GTTGTTGATG GAGAGGTTCT TCAGGAATGA GTGCCGCAGC 
GCCGTGCGCA TCAAGGTGTT GGATGTTCTG TCCTTCGTGC TGCTGATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCGCAGC TCTCCCACAT TCCCGAGGAT 
AAGGACCATC AGGTCCGAAA GCTGGCTACT CAGCTGCTGG TGGACCTGGC AGAGGGGTGC 
CACACCCACC ACTTCAACAG TCTGCTGGAC ATCATTGAAA AGGTGATGGC GCGCTCACTC 
TCTCCACCCC CGGAGCTGGA AGAAAGGGAC CTGGCCATGC ACTCGGCCTC CCTGGAGGAC 
GTGAAGACCG CGGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTCTA CACCTTGCCT 
GCCAGCCACG CCACTCGAGT GTATGAGAGC CTCATTAGTC ACATCCAGCT CCATTACAAG 
CACGGCTACT CCCTGCCCAT TGCTAGCAGC ATCCGACTAC AGGCCTTTGA CTTCCTGCTG 
CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGAGA 
TTCAGCCCTT ACTGCCTCTG TGACTGCATG GAACTGGATA GAGCCTCAGA GAAGAAAGCC 
AGTGGGCCCC TTTCACCTCC AACTGGGCCC CCTAGCCCTG TGCCTATGGG CCCTGCTGTG 
CGGCTCGGCT ACCTACCCTA CTCCCTGCTC TTCCGTGTCC TGTTGCAGTG TTTGAAGCAG 
GAGAGCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAGGC TGCCGGAGTC ACTGCGCTAC 
AAAGTCCTCA TCTTCACCTC CCCCTGCAGT GTCGACCAGC TGTCTTCTGC CCTCTGCTCC 
ATGCTTTCAG CTCCAAAGAC CCTTGAGCGG CTCCGAGGTA CCCCAGAAGG CTTCTCGAGA 
ACTGACCTGC ACTTGGCTGT GGTTCCCGTG CTAACAGCAT TAATATCTTA TCACAACTAT 
TTGGACAAGA CCAGACAGCG TGAGATGGTG TACTGCCTGG AACAAGGCCT TATCTACCGC 
TGCGCCAGCC AGTGCGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC 
ATCAAGGCGC TTCCTGTCCT GGTGGTGAAG CTCACACACA TTTCTGCCAC AGCCAGCATG 
GCCATCCCGC TCCTGGAGTT CCTGTCTACT TTGGCCAGGC TGCCCCACCT CTACAGGAAC 
TTTGCTGCAG AGCAGTATGC CAGCGTGTTT GCCATCTCCT TGCCATACAC CAACCCCTCC 
AAGTTCAACC AGTACATTGT GTGTCTGGCT CACCACGTCA TAGCCATGTG GTTCATCAGG 
TGCCGACTGC CCTTTCGGAA GGATTTCGTC CCTTATATCA CTAAGGGTTT GCGTTCCAAT 
GTCCTCCTGT CTTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT 
CTTAATGAGA GACCCAAAAG CAGAATACAG ACATCCCTTA CGAGCGCCAG CCTGGGGTCT 
GCGGATGAGA ACTCTATGGC CCAGGCTGAT GACAACTTGA AGAATCTCCA CTTGGAGCTC 
ACAGAAACAT GTCTGGACAT GATGGCCCGA TATGTGTTCT CCAATTTCAC TGCAGTCCCC 
AAGAGGTCCC CTGTGGGAGA GTTCCTCCTG GCAGGGGGTA GGACCAAAAC CTGGCTGGTT 
GGAAACAAGC TTGTCACTGT GACAACAAGT GTGGGAACTG GTACACGGTC GCTGCTGGGC 
CTGGACTCTG GGGACCTGCA GGGTGGCTCG GATTCAAGCT CTGATCCTAG CACACATGTG 
AGGCAGACAA AGGAAGCACC GGCCAAACTG GAGTCCCAGG CTGGGCAGCA GGTGTCCCGT 
GGGGCCCGGG ACCGGGTCCG CTCCATGTCA GGGGGCCATG GCCTTCGAGT TGGTGTCCTG 
GATACTTCAG CTCCCTATTC CCCAGGTGGC TCTGCTTCTC TGGGACCACA GACGGCGGTG 
GCAGCGAAGC CCGAGAAGCC CCCTGCAGGA GCCCAGCTTC CAACAGCAGA GAAGACGAAT 
CTGGCAGCCT ATGTGCCTTT GTTAACCCAG GGCTGGGCAG AAATCTTAGT CCGCAGACCC 
ACAGGAAACA CCAGCTGGCT GATGAGCTTG GAGAACCCGC TCAGCCCCTT CTCCTCAGAC 
ATCAACAACA TGCCCCTGCA AGAGCTGTCC AATGCCCTTA TGGCTGCTGA ACGTTTCAAG 
GAGCACCGGG ACACGGCCCT GTACAAGTCA TTGTCAGTGC CAGCGGCTGG CACCGCCAAG 
CCTCCTACTC TCCCACGCTC TAATACAGAC TCAGCCATGG TCCTTGAGGA GGGAAGTCCA 
GGAGAGACTC AGGTGCCAGT GGAGCCCCCC GAATTGGAGG ATTTCGAGGC GGCGTTAGGT 
ACAGACAGGC ACTGCCAGCG GCCTGACACC TACAGCCGGT CATCCTCAGC ATCTAGCCAG 
GAAGAAAAGT CCCATTTGGA GGAGCTTGCT GCAGGGGGTA TCCCCATTGA GCGGGCCATC 
TCCTCTGAGG GGGCTCGGCC TGCCGTGGAC CTCTCCTTCC AACCCTCACA GCCTTTGAGC 
AAGTCTAGCT CTTCTCCGGA GTTGCAGACC TTACAGGACA TCCTTGGAGA CCTAGGGGAC 
AAGATTGATA TTGGACGGCT GAGTCCTGAG GCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG CAGCTACCTG GTCAGCTACG GGTGAAGAGA GCCGTATCAC AGTCCCACCT 
GAAGGTCCTC TGCCTTCCAG TTCTCCCCGC TCCCCCAGTG GCCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCCGCTCC ATCACGAAGG GGAAAGAGGG TAGAAAGGGA TAACTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAG GTGCCAGGCA TCAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCATTCGCC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTATTGCCC 
AATGAGTCCT TTGAACGGTC AGTACAGCTG CTTGACCAGA TTCCATCCTA TGACACTCAC 
AAGATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAGCA GTGAGCTGGC CATCCTGTCA 
AATGAGCATG GCTCTTACAG GTACACGGAG TTTCTGACAG GCCTGGGTCG GCTTATTGAG 
CTCAAGGACT GCCAACCAGA CAAGGTGTAC TTAGGTGGAC TGGATGTATG TGGCGAGGAT 
GGACAGTTCA CCTACTGCTG GCATGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GACTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAACTGGG CACCATTAAG 
GGCCAGTTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAGTG CAACCTATTG 
ACCCTGCAGT GCAGGAAAGA CATGGAAGGC CTCGTGGACA CCAGTGTGGC CAAGATTGTG 
TCTGACCGCA ACCTGTCCTT TGTGGCCCGA CAGATGGCCC TGCATGCAAA TATGGCCTCA 
CAGGTACACC ACAGCCGATC CAACCCCACT GACATCTATC CCTCCAAGTG GATCGCAAGA 
CTCCGCCACA TTAAGCGTCT CCGCCAGAGG ATCCGTGAAG AGGTGCACTA TTCCAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGCTCAAGC CCCTGAGGCT 
ACACCCACCT ATGAAACAGG CCAGCGGAAG CGCCTCATTT CCTCCGTGGA TGACTTCACA 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_030249650.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105510.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X12, mRNA.

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CloneID	OMu87150
Clone ID Related Accession (Same CDS sequence)	XM_030249650.1 , XM_030249651.1
Accession Version	XM_030249650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105510.1
CDS	300..5546
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_030249650.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249650.1

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_030249651.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105511.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X13, mRNA.

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CloneID	OMu87150
Clone ID Related Accession (Same CDS sequence)	XM_030249650.1 , XM_030249651.1
Accession Version	XM_030249651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105511.1
CDS	244..5490
Translation	MIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_030249651.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249651.1

ORF Insert Sequence:

ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAGC TTGAGGAGCA TGCAGTGGAG 
GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCAGA GGCCCGGCAT 
GCAGTTCTCA CCTTATTGAA GGCCATTGTA CAGGGACAGG GTGATCGTTT GGGAGTTCTC 
AGAGCCCTCT TCTTCAAAGT GATCAAGGAC TACCCCTCCA ATGAAGACCT CCATGAAAGG 
CTAGAAGTTT TTAAGGCCCT CACAGACAAT GGGAGGCACA TTACCTATTT GGAAGAAGAA 
CTGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( NM_001286720.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273649.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OMu18371
Clone ID Related Accession (Same CDS sequence)	NM_001286720.1
Accession Version	NM_001286720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	tuberin isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147908.1, AK147487.1, AK163991.1 and AK147302.1. Transcript Variant: This variant (7) has an alternate splice site and lacks a splice junction in the 3' coding region, compared to variant 3. The resulting isoform (5) is longer and has an alternate segment in the C-terminal region, compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGATGGCC CTGCATGCAA ATGTGAGTGG 
TGGCGGCAGC CTGGGGAGAT AGTCGTGTGG GCGCTCCCTG TTGTGATGGA GCTCACTGTC 
ACCATCCTGC TCTGTCACCT CCAGATGGCC TCACAGGTAC ACCACAGCCG ATCCAACCCC 
ACTGACATCT ATCCCTCCAA GTGGATCGCA AGACTCCGCC ACATTAAGCG TCTCCGCCAG 
AGGATCCGTG AAGAGGTGCA CTATTCCAAC CCAAGCTTGC CTCTGATGCA CCCTCCAGCC 
CACACCAAAG CCCCAGCTCA AGCCCCTGAG GCTACACCCA CCTATGAAAC AGGCCAGCGG 
AAGCGCCTCA TTTCCTCCGT GGATGACTTC ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273649.1
CDS	120..5564
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDGPACKCEWWRQPGEIVVWALPVVMELTVTILLCHLQMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	NM_001286720.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286720.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGATGGCC CTGCATGCAA ATGTGAGTGG 
TGGCGGCAGC CTGGGGAGAT AGTCGTGTGG GCGCTCCCTG TTGTGATGGA GCTCACTGTC 
ACCATCCTGC TCTGTCACCT CCAGATGGCC TCACAGGTAC ACCACAGCCG ATCCAACCCC 
ACTGACATCT ATCCCTCCAA GTGGATCGCA AGACTCCGCC ACATTAAGCG TCTCCGCCAG 
AGGATCCGTG AAGAGGTGCA CTATTCCAAC CCAAGCTTGC CTCTGATGCA CCCTCCAGCC 
CACACCAAAG CCCCAGCTCA AGCCCCTGAG GCTACACCCA CCTATGAAAC AGGCCAGCGG 
AAGCGCCTCA TTTCCTCCGT GGATGACTTC ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( NM_001286713.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273642.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 3, mRNA.

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CloneID	OMu21891
Clone ID Related Accession (Same CDS sequence)	XM_017317384.2 , NM_001286713.1 , XM_017317384.1
Accession Version	NM_001286713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	tuberin isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147908.1, AK147487.1 and AK147302.1. Transcript Variant: This variant (3) represents all exons of this gene and encodes a 1808aa isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147487.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273642.1
CDS	120..5546
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	NM_001286713.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286713.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( NM_001286714.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273643.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 4, mRNA.

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CloneID	OMu23154
Clone ID Related Accession (Same CDS sequence)	NM_001286714.1 , NM_011647.3
Accession Version	NM_001286714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	tuberin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147908.1, BC052449.1, AK148062.1 and AK147302.1. Transcript Variant: This variant (4) has an alternate splice site in the 5' UTR and lacks an in-frame exon in the coding region, compared to variant 3. The resulting isoform (1) lacks an internal segment, compared to isoform 3. Variants 1 and 4 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273643.1
CDS	115..5472
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	NM_001286714.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286714.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( NM_011647.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035777.2 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 1, mRNA.

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CloneID	OMu23154
Clone ID Related Accession (Same CDS sequence)	NM_001286714.1 , NM_011647.3
Accession Version	NM_011647.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	tuberin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143799.1, AK147908.1 and AK147302.1. On Nov 14, 2013 this sequence version replaced NM_011647.2. Transcript Variant: This variant (1) lacks an in-frame exon in the coding region, compared to variant 3. The resulting isoform (1) lacks an internal segment, compared to isoform 3. Variants 1 and 4 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147302.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035777.2
CDS	120..5477
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	NM_011647.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011647.3

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGACTCAG CCATGGTCCT TGAGGAGGGA 
AGTCCAGGAG AGACTCAGGT GCCAGTGGAG CCCCCCGAAT TGGAGGATTT CGAGGCGGCG 
TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACACCTACA GCCGGTCATC CTCAGCATCT 
AGCCAGGAAG AAAAGTCCCA TTTGGAGGAG CTTGCTGCAG GGGGTATCCC CATTGAGCGG 
GCCATCTCCT CTGAGGGGGC TCGGCCTGCC GTGGACCTCT CCTTCCAACC CTCACAGCCT 
TTGAGCAAGT CTAGCTCTTC TCCGGAGTTG CAGACCTTAC AGGACATCCT TGGAGACCTA 
GGGGACAAGA TTGATATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTACGGGTG AAGAGAGCCG TATCACAGTC 
CCACCTGAAG GTCCTCTGCC TTCCAGTTCT CCCCGCTCCC CCAGTGGCCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC CGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGATAAC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAGGTGC CAGGCATCAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA TTCGCCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTA 
TTGCCCAATG AGTCCTTTGA ACGGTCAGTA CAGCTGCTTG ACCAGATTCC ATCCTATGAC 
ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC 
CTGTCAAATG AGCATGGCTC TTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGTCGGCTT 
ATTGAGCTCA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTATGTGGC 
GAGGATGGAC AGTTCACCTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA ACTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAGTGCAAC 
CTATTGACCC TGCAGTGCAG GAAAGACATG GAAGGCCTCG TGGACACCAG TGTGGCCAAG 
ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG 
GCCTCACAGG TACACCACAG CCGATCCAAC CCCACTGACA TCTATCCCTC CAAGTGGATC 
GCAAGACTCC GCCACATTAA GCGTCTCCGC CAGAGGATCC GTGAAGAGGT GCACTATTCC 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGC TCAAGCCCCT 
GAGGCTACAC CCACCTATGA AACAGGCCAG CGGAAGCGCC TCATTTCCTC CGTGGATGAC 
TTCACAGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( NM_001286718.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273647.1 Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 6, mRNA.

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CloneID	OMu23173
Clone ID Related Accession (Same CDS sequence)	NM_001286718.1
Accession Version	NM_001286718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	tuberin isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143799.1, BC052449.1 and AK147302.1. Transcript Variant: This variant (6) has an alternate splice site and lacks two in-frame exons in the coding region, compared to variant 3. The resulting isoform (4) lacks two internal segments, compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143799.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGACTCAGCC ATGGTCCTTG AGGAGGGAAG TCCAGGAGAG 
ACTCAGGTGC CAGTGGAGCC CCCCGAATTG GAGGATTTCG AGGCGGCGTT AGGTACAGAC 
AGGCACTGCC AGCGGCCTGA CACCTACAGC CGGTCATCCT CAGCATCTAG CCAGGAAGAA 
AAGTCCCATT TGGAGGAGCT TGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT 
GAGGGGGCTC GGCCTGCCGT GGACCTCTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT 
AGCTCTTCTC CGGAGTTGCA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGATT 
GATATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGGTCCC AGTCAGGGAT CCTGGATGGG 
GAAGCAGCTA CCTGGTCAGC TACGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT 
CCTCTGCCTT CCAGTTCTCC CCGCTCCCCC AGTGGCCTCC GGCCCCGAGG CTATACCATC 
TCTGATTCCG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGATAACTT CAAGAGCAGA 
GCTGCGGCCT CCAGTGCTGA GAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG 
CTCTACCATT CGCCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTATT GCCCAATGAG 
TCCTTTGAAC GGTCAGTACA GCTGCTTGAC CAGATTCCAT CCTATGACAC TCACAAGATT 
GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT GTCAAATGAG 
CATGGCTCTT ACAGGTACAC GGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTCAAG 
GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TATGTGGCGA GGATGGACAG 
TTCACCTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC CACCCTGATG 
CCCACCAAGG ATGTGGACAA GCACCGCTGT GACAAGAAAC GGCACCTGGG CAATGACTTT 
GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAAC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCATGTGAT CATCACACCG CTGGACTACA AGTGCAACCT ATTGACCCTG 
CAGTGCAGGA AAGACATGGA AGGCCTCGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC 
CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA 
CACCACAGCC GATCCAACCC CACTGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC 
CACATTAAGC GTCTCCGCCA GAGGATCCGT GAAGAGGTGC ACTATTCCAA CCCAAGCTTG 
CCTCTGATGC ACCCTCCAGC CCACACCAAA GCCCCAGCTC AAGCCCCTGA GGCTACACCC 
ACCTATGAAA CAGGCCAGCG GAAGCGCCTC ATTTCCTCCG TGGATGACTT CACAGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273647.1
CDS	120..5345
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	NM_001286718.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSC complex subunit 2 (Tsc2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286718.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGACTCAGCC ATGGTCCTTG AGGAGGGAAG TCCAGGAGAG 
ACTCAGGTGC CAGTGGAGCC CCCCGAATTG GAGGATTTCG AGGCGGCGTT AGGTACAGAC 
AGGCACTGCC AGCGGCCTGA CACCTACAGC CGGTCATCCT CAGCATCTAG CCAGGAAGAA 
AAGTCCCATT TGGAGGAGCT TGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT 
GAGGGGGCTC GGCCTGCCGT GGACCTCTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT 
AGCTCTTCTC CGGAGTTGCA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGATT 
GATATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGGTCCC AGTCAGGGAT CCTGGATGGG 
GAAGCAGCTA CCTGGTCAGC TACGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT 
CCTCTGCCTT CCAGTTCTCC CCGCTCCCCC AGTGGCCTCC GGCCCCGAGG CTATACCATC 
TCTGATTCCG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGATAACTT CAAGAGCAGA 
GCTGCGGCCT CCAGTGCTGA GAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG 
CTCTACCATT CGCCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTATT GCCCAATGAG 
TCCTTTGAAC GGTCAGTACA GCTGCTTGAC CAGATTCCAT CCTATGACAC TCACAAGATT 
GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT GTCAAATGAG 
CATGGCTCTT ACAGGTACAC GGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTCAAG 
GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TATGTGGCGA GGATGGACAG 
TTCACCTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC CACCCTGATG 
CCCACCAAGG ATGTGGACAA GCACCGCTGT GACAAGAAAC GGCACCTGGG CAATGACTTT 
GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAAC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCATGTGAT CATCACACCG CTGGACTACA AGTGCAACCT ATTGACCCTG 
CAGTGCAGGA AAGACATGGA AGGCCTCGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC 
CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA 
CACCACAGCC GATCCAACCC CACTGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC 
CACATTAAGC GTCTCCGCCA GAGGATCCGT GAAGAGGTGC ACTATTCCAA CCCAAGCTTG 
CCTCTGATGC ACCCTCCAGC CCACACCAAA GCCCCAGCTC AAGCCCCTGA GGCTACACCC 
ACCTATGAAA CAGGCCAGCG GAAGCGCCTC ATTTCCTCCG TGGATGACTT CACAGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_017317384.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172873.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.

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CloneID	OMu21891
Clone ID Related Accession (Same CDS sequence)	XM_017317384.2 , NM_001286713.1 , XM_017317384.1
Accession Version	XM_017317384.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172873.1
CDS	108..5534
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTVASFSSLYQPSCQGQLHRSVSWADSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_017317384.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317384.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTT 
GTCAACCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTATGCAT CCGGACAGTG TCCTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTGCTGGATG CTGTGGTCTG CTACAACTGC TTACCAGCCG AGAGCCTGCC TCTGTTCATT 
ATCACCCTGT GCCGCACCAT CAATGTCAAG GAGCTGTGTG AGCCTTGCTG GAAGTTGATG 
CGTAACCTTC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCGCATCATG 
GAGGACAGAT CCTACATGGA AGATGCCCCA CTGCTGAGAG GAGCTGTGTT CTTTGTAGGG 
ATGGCACTCT GGGGAGCTCA CCGGCTCTAC TCTCTCAAGA ACTCCCCCAC ATCTGTGCTG 
CCGTCTTTTT ATGAGGCTAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGG AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TCATTGAACG ACTACTTCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC 
AAGACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TATGTGACCA GAACGAGTTC 
CATGGCTCGC AGGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAA 
TCCTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAGTCCA TCCACCCTGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTGATGGAG AGGTTCTTCA GGAATGAGTG CCGCAGCGCC 
GTGCGCATCA AGGTGTTGGA TGTTCTGTCC TTCGTGCTGC TGATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCGCAGCTCT CCCACATTCC CGAGGATAAG 
GACCATCAGG TCCGAAAGCT GGCTACTCAG CTGCTGGTGG ACCTGGCAGA GGGGTGCCAC 
ACCCACCACT TCAACAGTCT GCTGGACATC ATTGAAAAGG TGATGGCGCG CTCACTCTCT 
CCACCCCCGG AGCTGGAAGA AAGGGACCTG GCCATGCACT CGGCCTCCCT GGAGGACGTG 
AAGACCGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTCTACAC CTTGCCTGCC 
AGCCACGCCA CTCGAGTGTA TGAGAGCCTC ATTAGTCACA TCCAGCTCCA TTACAAGCAC 
GGCTACTCCC TGCCCATTGC TAGCAGCATC CGACTACAGG CCTTTGACTT CCTGCTGCTA 
CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GCCTCTGTGA CTGCATGGAA CTGGATAGAG CCTCAGAGAA GAAAGCCAGT 
GGGCCCCTTT CACCTCCAAC TGGGCCCCCT AGCCCTGTGC CTATGGGCCC TGCTGTGCGG 
CTCGGCTACC TACCCTACTC CCTGCTCTTC CGTGTCCTGT TGCAGTGTTT GAAGCAGGAG 
AGCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAGGCTGC CGGAGTCACT GCGCTACAAA 
GTCCTCATCT TCACCTCCCC CTGCAGTGTC GACCAGCTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGCTC CAAAGACCCT TGAGCGGCTC CGAGGTACCC CAGAAGGCTT CTCGAGAACT 
GACCTGCACT TGGCTGTGGT TCCCGTGCTA ACAGCATTAA TATCTTATCA CAACTATTTG 
GACAAGACCA GACAGCGTGA GATGGTGTAC TGCCTGGAAC AAGGCCTTAT CTACCGCTGC 
GCCAGCCAGT GCGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTTC CTGTCCTGGT GGTGAAGCTC ACACACATTT CTGCCACAGC CAGCATGGCC 
ATCCCGCTCC TGGAGTTCCT GTCTACTTTG GCCAGGCTGC CCCACCTCTA CAGGAACTTT 
GCTGCAGAGC AGTATGCCAG CGTGTTTGCC ATCTCCTTGC CATACACCAA CCCCTCCAAG 
TTCAACCAGT ACATTGTGTG TCTGGCTCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGACTGCCCT TTCGGAAGGA TTTCGTCCCT TATATCACTA AGGGTTTGCG TTCCAATGTC 
CTCCTGTCTT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT 
AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CCCTTACGAG CGCCAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA TTTCACTGCA 
GTCCCCAAGA GGTCCCCTGT GGGAGAGTTC CTCCTGGCAG GGGGTAGGAC CAAAACCTGG 
CTGGTTGGAA ACAAGCTTGT CACTGTGACA ACAAGTGTGG GAACTGGTAC ACGGTCGCTG 
CTGGGCCTGG ACTCTGGGGA CCTGCAGGGT GGCTCGGATT CAAGCTCTGA TCCTAGCACA 
CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GCCATGGCCT TCGAGTTGGT 
GTCCTGGATA CTTCAGCTCC CTATTCCCCA GGTGGCTCTG CTTCTCTGGG ACCACAGACG 
GCGGTGGCAG CGAAGCCCGA GAAGCCCCCT GCAGGAGCCC AGCTTCCAAC AGCAGAGAAG 
ACGAATCTGG CAGCCTATGT GCCTTTGTTA ACCCAGGGCT GGGCAGAAAT CTTAGTCCGC 
AGACCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CCCTTATGGC TGCTGAACGT 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACC 
GCCAAGCCTC CTACTCTCCC ACGCTCTAAT ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCAGC CATGGTCCTT 
GAGGAGGGAA GTCCAGGAGA GACTCAGGTG CCAGTGGAGC CCCCCGAATT GGAGGATTTC 
GAGGCGGCGT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACACCTACAG CCGGTCATCC 
TCAGCATCTA GCCAGGAAGA AAAGTCCCAT TTGGAGGAGC TTGCTGCAGG GGGTATCCCC 
ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTGCCG TGGACCTCTC CTTCCAACCC 
TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCGGAGTTGC AGACCTTACA GGACATCCTT 
GGAGACCTAG GGGACAAGAT TGATATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGGTCC 
CAGTCAGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTACGGGTGA AGAGAGCCGT 
ATCACAGTCC CACCTGAAGG TCCTCTGCCT TCCAGTTCTC CCCGCTCCCC CAGTGGCCTC 
CGGCCCCGAG GCTATACCAT CTCTGATTCC GCTCCATCAC GAAGGGGAAA GAGGGTAGAA 
AGGGATAACT TCAAGAGCAG AGCTGCGGCC TCCAGTGCTG AGAAGGTGCC AGGCATCAAC 
CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCGCCCTTTT TTGGTGATGA GTCCAATAAG 
CCCATCCTAT TGCCCAATGA GTCCTTTGAA CGGTCAGTAC AGCTGCTTGA CCAGATTCCA 
TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG 
CTGGCCATCC TGTCAAATGA GCATGGCTCT TACAGGTACA CGGAGTTTCT GACAGGCCTG 
GGTCGGCTTA TTGAGCTCAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT 
GTATGTGGCG AGGATGGACA GTTCACCTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT 
TTCCACATTG CCACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGCTG TGACAAGAAA 
CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAA 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCATGTGA TCATCACACC GCTGGACTAC 
AAGTGCAACC TATTGACCCT GCAGTGCAGG AAAGACATGG AAGGCCTCGT GGACACCAGT 
GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT 
GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACTGACAT CTATCCCTCC 
AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGCC AGAGGATCCG TGAAGAGGTG 
CACTATTCCA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCACACCAA AGCCCCAGCT 
CAAGCCCCTG AGGCTACACC CACCTATGAA ACAGGCCAGC GGAAGCGCCT CATTTCCTCC 
GTGGATGACT TCACAGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_006524040.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524103.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.

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CloneID	OMu53425
Clone ID Related Accession (Same CDS sequence)	XM_006524040.2 , XM_006524040.3
Accession Version	XM_006524040.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524103.1
CDS	114..5468
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_006524040.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524040.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA 
CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT 
GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCCC TTACGAGCGC CAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAATTT CACTGCAGTC 
CCCAAGAGGT CCCCTGTGGG AGAGTTCCTC CTGGCAGGGG GTAGGACCAA AACCTGGCTG 
GTTGGAAACA AGCTTGTCAC TGTGACAACA AGTGTGGGAA CTGGTACACG GTCGCTGCTG 
GGCCTGGACT CTGGGGACCT GCAGGGTGGC TCGGATTCAA GCTCTGATCC TAGCACACAT 
GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGCC ATGGCCTTCG AGTTGGTGTC 
CTGGATACTT CAGCTCCCTA TTCCCCAGGT GGCTCTGCTT CTCTGGGACC ACAGACGGCG 
GTGGCAGCGA AGCCCGAGAA GCCCCCTGCA GGAGCCCAGC TTCCAACAGC AGAGAAGACG 
AATCTGGCAG CCTATGTGCC TTTGTTAACC CAGGGCTGGG CAGAAATCTT AGTCCGCAGA 
CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCCC TTATGGCTGC TGAACGTTTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACCGCC 
AAGCCTCCTA CTCTCCCACG CTCTAATACA GACTCAGCCA TGGTCCTTGA GGAGGGAAGT 
CCAGGAGAGA CTCAGGTGCC AGTGGAGCCC CCCGAATTGG AGGATTTCGA GGCGGCGTTA 
GGTACAGACA GGCACTGCCA GCGGCCTGAC ACCTACAGCC GGTCATCCTC AGCATCTAGC 
CAGGAAGAAA AGTCCCATTT GGAGGAGCTT GCTGCAGGGG GTATCCCCAT TGAGCGGGCC 
ATCTCCTCTG AGGGGGCTCG GCCTGCCGTG GACCTCTCCT TCCAACCCTC ACAGCCTTTG 
AGCAAGTCTA GCTCTTCTCC GGAGTTGCAG ACCTTACAGG ACATCCTTGG AGACCTAGGG 
GACAAGATTG ATATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGGTCCCA GTCAGGGATC 
CTGGATGGGG AAGCAGCTAC CTGGTCAGCT ACGGGTGAAG AGAGCCGTAT CACAGTCCCA 
CCTGAAGGTC CTCTGCCTTC CAGTTCTCCC CGCTCCCCCA GTGGCCTCCG GCCCCGAGGC 
TATACCATCT CTGATTCCGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGATAACTTC 
AAGAGCAGAG CTGCGGCCTC CAGTGCTGAG AAGGTGCCAG GCATCAACCC TAGCTTTGTG 
TTCCTACAGC TCTACCATTC GCCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTATTG 
CCCAATGAGT CCTTTGAACG GTCAGTACAG CTGCTTGACC AGATTCCATC CTATGACACT 
CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTG 
TCAAATGAGC ATGGCTCTTA CAGGTACACG GAGTTTCTGA CAGGCCTGGG TCGGCTTATT 
GAGCTCAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT ATGTGGCGAG 
GATGGACAGT TCACCTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCC 
ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGCTGTG ACAAGAAACG GCACCTGGGC 
AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAACT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCATGTGATC ATCACACCGC TGGACTACAA GTGCAACCTA 
TTGACCCTGC AGTGCAGGAA AGACATGGAA GGCCTCGTGG ACACCAGTGT GGCCAAGATT 
GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC 
TCACAGGTAC ACCACAGCCG ATCCAACCCC ACTGACATCT ATCCCTCCAA GTGGATCGCA 
AGACTCCGCC ACATTAAGCG TCTCCGCCAG AGGATCCGTG AAGAGGTGCA CTATTCCAAC 
CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CACACCAAAG CCCCAGCTCA AGCCCCTGAG 
GCTACACCCA CCTATGAAAC AGGCCAGCGG AAGCGCCTCA TTTCCTCCGT GGATGACTTC 
ACAGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_006524044.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524107.1 Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X10, mRNA.

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CloneID	OMu53428
Clone ID Related Accession (Same CDS sequence)	XM_006524044.2 , XM_006524044.3
Accession Version	XM_006524044.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGCAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGACTCAGCC ATGGTCCTTG AGGAGGGAAG TCCAGGAGAG 
ACTCAGGTGC CAGTGGAGCC CCCCGAATTG GAGGATTTCG AGGCGGCGTT AGGTACAGAC 
AGGCACTGCC AGCGGCCTGA CACCTACAGC CGGTCATCCT CAGCATCTAG CCAGGAAGAA 
AAGTCCCATT TGGAGGAGCT TGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT 
GAGGGGGCTC GGCCTGCCGT GGACCTCTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT 
AGCTCTTCTC CGGAGTTGCA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGATT 
GATATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGGTCCC AGTCAGGGAT CCTGGATGGG 
GAAGCAGCTA CCTGGTCAGC TACGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT 
CCTCTGCCTT CCAGTTCTCC CCGCTCCCCC AGTGGCCTCC GGCCCCGAGG CTATACCATC 
TCTGATTCCG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGATAACTT CAAGAGCAGA 
GCTGCGGCCT CCAGTGCTGA GAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG 
CTCTACCATT CGCCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTATT GCCCAATGAG 
TCCTTTGAAC GGTCAGTACA GCTGCTTGAC CAGATTCCAT CCTATGACAC TCACAAGATT 
GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT GTCAAATGAG 
CATGGCTCTT ACAGGTACAC GGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTCAAG 
GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TATGTGGCGA GGATGGACAG 
TTCACCTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC CACCCTGATG 
CCCACCAAGG ATGTGGACAA GCACCGCTGT GACAAGAAAC GGCACCTGGG CAATGACTTT 
GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAAC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCATGTGAT CATCACACCG CTGGACTACA AGTGCAACCT ATTGACCCTG 
CAGTGCAGGA AAGACATGGA AGGCCTCGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC 
CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA 
CACCACAGCC GATCCAACCC CACTGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC 
CACATTAAGC GTCTCCGCCA GAGGATCCGT GAAGAGGTGC ACTATTCCAA CCCAAGCTTG 
CCTCTGATGC ACCCTCCAGC CCACACCAAA GCCCCAGCTC AAGCCCCTGA GGCTACACCC 
ACCTATGAAA CAGGCCAGCG GAAGCGCCTC ATTTCCTCCG TGGATGACTT CACAGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524107.1
CDS	114..5339
Translation	MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_006524044.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524044.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCCGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
TTGGGAACAT CGAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACTGA GTTTATCATC 
ACATCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG CATCCGAATG 
ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAGCTTG AGGAGCATGC AGTGGAGGCA 
CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCAGAGGC CCGGCATGCA 
GTTCTCACCT TATTGAAGGC CATTGTACAG GGACAGGGTG ATCGTTTGGG AGTTCTCAGA 
GCCCTCTTCT TCAAAGTGAT CAAGGACTAC CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGG AGGCACATTA CCTATTTGGA AGAAGAACTG 
GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTTGTC 
AACCTGGTCA AGTTCAACAG CTGTTACCTT GACGAATACA TTGCATCAAT GGTTCACATG 
ATTTGTCTGC TATGCATCCG GACAGTGTCC TCTGTGGACA TTGAGGTGTC CTTGCAAGTG 
CTGGATGCTG TGGTCTGCTA CAACTGCTTA CCAGCCGAGA GCCTGCCTCT GTTCATTATC 
ACCCTGTGCC GCACCATCAA TGTCAAGGAG CTGTGTGAGC CTTGCTGGAA GTTGATGCGT 
AACCTTCTGG GCACCCACCT GGGCCACAGC GCCATCTACA ACATGTGCCG CATCATGGAG 
GACAGATCCT ACATGGAAGA TGCCCCACTG CTGAGAGGAG CTGTGTTCTT TGTAGGGATG 
GCACTCTGGG GAGCTCACCG GCTCTACTCT CTCAAGAACT CCCCCACATC TGTGCTGCCG 
TCTTTTTATG AGGCTATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC 
ATAACAAGAC TCATCAAGAA GTATAGGAAG GAGCTCCAGG CTGTGACATG GGATATTCTG 
CTGGACATCA TTGAACGACT ACTTCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAAG 
ACCATCGTCC ATGACCTGCT GACCACTGTG GAGGAGCTAT GTGACCAGAA CGAGTTCCAT 
GGCTCGCAGG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG ACCTGAATCC 
TCTCTCTTAA ACTTGATATC CTACAGAGCC CAGTCCATCC ACCCTGCCAA GGATGGCTGG 
ATCCAGAACT TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ATGAGTGCCG CAGCGCCGTG 
CGCATCAAGG TGTTGGATGT TCTGTCCTTC GTGCTGCTGA TCAACAGGCA GTTCTATGAG 
GAGGAGCTGA TTAACTCGGT GGTCATCTCG CAGCTCTCCC ACATTCCCGA GGATAAGGAC 
CATCAGGTCC GAAAGCTGGC TACTCAGCTG CTGGTGGACC TGGCAGAGGG GTGCCACACC 
CACCACTTCA ACAGTCTGCT GGACATCATT GAAAAGGTGA TGGCGCGCTC ACTCTCTCCA 
CCCCCGGAGC TGGAAGAAAG GGACCTGGCC ATGCACTCGG CCTCCCTGGA GGACGTGAAG 
ACCGCGGTCC TGGGGCTCCT GGTCATCCTT CAGACCAAGC TCTACACCTT GCCTGCCAGC 
CACGCCACTC GAGTGTATGA GAGCCTCATT AGTCACATCC AGCTCCATTA CAAGCACGGC 
TACTCCCTGC CCATTGCTAG CAGCATCCGA CTACAGGCCT TTGACTTCCT GCTGCTACTG 
CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GAGATTCAGC 
CCTTACTGCC TCTGTGACTG CATGGAACTG GATAGAGCCT CAGAGAAGAA AGCCAGTGGG 
CCCCTTTCAC CTCCAACTGG GCCCCCTAGC CCTGTGCCTA TGGGCCCTGC TGTGCGGCTC 
GGCTACCTAC CCTACTCCCT GCTCTTCCGT GTCCTGTTGC AGTGTTTGAA GCAGGAGAGC 
GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AGGCTGCCGG AGTCACTGCG CTACAAAGTC 
CTCATCTTCA CCTCCCCCTG CAGTGTCGAC CAGCTGTCTT CTGCCCTCTG CTCCATGCTT 
TCAGCTCCAA AGACCCTTGA GCGGCTCCGA GGTACCCCAG AAGGCTTCTC GAGAACTGAC 
CTGCACTTGG CTGTGGTTCC CGTGCTAACA GCATTAATAT CTTATCACAA CTATTTGGAC 
AAGACCAGAC AGCGTGAGAT GGTGTACTGC CTGGAACAAG GCCTTATCTA CCGCTGCGCC 
AGCCAGTGCG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG 
GCGCTTCCTG TCCTGGTGGT GAAGCTCACA CACATTTCTG CCACAGCCAG CATGGCCATC 
CCGCTCCTGG AGTTCCTGTC TACTTTGGCC AGGCTGCCCC ACCTCTACAG GAACTTTGCT 
GCAGAGCAGT ATGCCAGCGT GTTTGCCATC TCCTTGCCAT ACACCAACCC CTCCAAGTTC 
AACCAGTACA TTGTGTGTCT GGCTCACCAC GTCATAGCCA TGTGGTTCAT CAGGTGCCGA 
CTGCCCTTTC GGAAGGATTT CGTCCCTTAT ATCACTAAGG GTTTGCGTTC CAATGTCCTC 
CTGTCTTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT 
GAGAGACCCA AAAGCAGAAT ACAGACATCC CTTACGAGCG CCAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAAC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAATT TCACTGCAGT CCCCAAGAGG 
TCCCCTGTGG GAGAGTTCCT CCTGGCAGGG GGTAGGACCA AAACCTGGCT GGTTGGAAAC 
AAGCTTGTCA CTGTGACAAC AAGTGTGGGA ACTGGTACAC GGTCGCTGCT GGGCCTGGAC 
TCTGGGGACC TGCAGGGTGG CTCGGATTCA AGCTCTGATC CTAGCACACA TGTGAGGCAG 
ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGC CATGGCCTTC GAGTTGGTGT CCTGGATACT 
TCAGCTCCCT ATTCCCCAGG TGGCTCTGCT TCTCTGGGAC CACAGACGGC GGTGGCAGCG 
AAGCCCGAGA AGCCCCCTGC AGGAGCCCAG CTTCCAACAG CAGAGAAGAC GAATCTGGCA 
GCCTATGTGC CTTTGTTAAC CCAGGGCTGG GCAGAAATCT TAGTCCGCAG ACCCACAGGA 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCC CTTATGGCTG CTGAACGTTT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACCGC CAAGCCTCCT 
ACTCTCCCAC GCTCTAATAC AGACTCAGCC ATGGTCCTTG AGGAGGGAAG TCCAGGAGAG 
ACTCAGGTGC CAGTGGAGCC CCCCGAATTG GAGGATTTCG AGGCGGCGTT AGGTACAGAC 
AGGCACTGCC AGCGGCCTGA CACCTACAGC CGGTCATCCT CAGCATCTAG CCAGGAAGAA 
AAGTCCCATT TGGAGGAGCT TGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT 
GAGGGGGCTC GGCCTGCCGT GGACCTCTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT 
AGCTCTTCTC CGGAGTTGCA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGATT 
GATATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGGTCCC AGTCAGGGAT CCTGGATGGG 
GAAGCAGCTA CCTGGTCAGC TACGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT 
CCTCTGCCTT CCAGTTCTCC CCGCTCCCCC AGTGGCCTCC GGCCCCGAGG CTATACCATC 
TCTGATTCCG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGATAACTT CAAGAGCAGA 
GCTGCGGCCT CCAGTGCTGA GAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG 
CTCTACCATT CGCCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTATT GCCCAATGAG 
TCCTTTGAAC GGTCAGTACA GCTGCTTGAC CAGATTCCAT CCTATGACAC TCACAAGATT 
GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT GTCAAATGAG 
CATGGCTCTT ACAGGTACAC GGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTCAAG 
GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TATGTGGCGA GGATGGACAG 
TTCACCTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC CACCCTGATG 
CCCACCAAGG ATGTGGACAA GCACCGCTGT GACAAGAAAC GGCACCTGGG CAATGACTTT 
GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAAC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCATGTGAT CATCACACCG CTGGACTACA AGTGCAACCT ATTGACCCTG 
CAGTGCAGGA AAGACATGGA AGGCCTCGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC 
CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA 
CACCACAGCC GATCCAACCC CACTGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC 
CACATTAAGC GTCTCCGCCA GAGGATCCGT GAAGAGGTGC ACTATTCCAA CCCAAGCTTG 
CCTCTGATGC ACCCTCCAGC CCACACCAAA GCCCCAGCTC AAGCCCCTGA GGCTACACCC 
ACCTATGAAA CAGGCCAGCG GAAGCGCCTC ATTTCCTCCG TGGATGACTT CACAGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Tsc2
Entrez Gene ID	22084
Full Name	tuberous sclerosis 2
Synonyms	Nafld,Tcs2
General protein information	Preferred Names tuberous sclerosis 2 Names tuberin tuberous sclerosis 2 protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 12.41 cM

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Tsc2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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