Tpst1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpst1
Entrez Gene ID	22021
Full Name	protein-tyrosine sulfotransferase 1
Synonyms	R75054,Tango13a
General protein information	Preferred Names protein-tyrosine sulfotransferase 1 Names protein-tyrosine sulfotransferase 1 TPST-1 tyrosylprotein sulfotransferase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_013837.2	NP_038865.1	protein-tyrosine sulfotransferase 1
XM_006504418.2	XP_006504481.1	protein-tyrosine sulfotransferase 1 isoform X1
NM_001130476.2	NP_001123948.1	protein-tyrosine sulfotransferase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TPST1	NP_003587.1
Pan troglodytes (chimpanzee)	TPST1	NP_001233396.1
Gallus gallus (chicken)	TPST1	XP_003642442.1
Caenorhabditis elegans (roundworm)	tpst-1	NP_499646.3
Xenopus tropicalis (tropical clawed frog)	tpst1	NP_001025640.1
	TPST1	XP_001086632.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000900	XP_316879.5
Mus musculus (house mouse)	Tpst1	NP_001123948.1
Rattus norvegicus (Norway rat)	Tpst1	NP_001011903.2
Drosophila melanogaster (fruit fly)	Tango13	NP_727717.1
Canis lupus familiaris (dog)	TPST1	XP_546906.2
Bos taurus (cattle)	TPST1	NP_001071380.1
Danio rerio (zebrafish)	tpst1	NP_571478.1

Gene Ontology
Bibliography

Related articles in PubMed

Salivary gland hypofunction in tyrosylprotein sulfotransferase-2 knockout mice is due to primary hypothyroidism.
Westmuckett AD, Siefert JC, Tesiram YA, Pinson DM, Moore KL
PloS one8(8)e71822(2013)

Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2.
Sherry DM, Kanan Y, Hamilton R, Hoffhines A, Arbogast KL, Fliesler SJ, Naash MI, Moore KL, Al-Ubaidi MR
PloS one7(6)e39702(2012)

Identification of novel innate immune genes by transcriptional profiling of macrophages stimulated with TLR ligands.
Yang IV, Jiang W, Rutledge HR, Lackford B, Warg LA, De Arras L, Alper S, Schwartz DA, Pisetsky DS
Molecular immunology48(15-16)1886-95(2011 Sep)

Tyrosine sulfation of native mouse Psgl-1 is required for optimal leukocyte rolling on P-selectin in vivo.
Westmuckett AD, Thacker KM, Moore KL
PloS one6(5)e20406(2011)

Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy.
Sherry DM, Murray AR, Kanan Y, Arbogast KL, Hamilton RA, Fliesler SJ, Burns ME, Moore KL, Al-Ubaidi MR
The European journal of neuroscience32(9)1461-72(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate the importance of protein-tyrosine sulfation for proper development of the retina and suggest different phenotypes resulting from elimination of TPST-1 or -2 may reflect differential expression patterns or levels of the enzymes.
Title: Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2.

two genes identified in this analysis, PLEC1 and TPST1, reduced IL-6 production by macrophages
Title: Identification of novel innate immune genes by transcriptional profiling of macrophages stimulated with TLR ligands.

Data show that Tpst1/Tpst2 DKO leukocytes bound less P-selectin than wild type leukocytes despite equivalent surface expression of Psgl-1.
Title: Tyrosine sulfation of native mouse Psgl-1 is required for optimal leukocyte rolling on P-selectin in vivo.

These results indicate that protein-tyrosine sulfation by Tpst1/2 is essential for proper outer segment morphogenesis and synaptic function, but is not critical for overall retinal structure or synapse formation.
Title: Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy.

Tpst1 and Tpst2 are the only Tpst genes in mice, tyrosine sulfation is required for normal pulmonary function at birth, and TPST-2 is required for normal thyroid gland function.
Title: Early postnatal pulmonary failure and primary hypothyroidism in mice with combined TPST-1 and TPST-2 deficiency.

The following Tpst1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpst1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu36142	XM_006504418.2 Latest version!	Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu18281	NM_013837.2 Latest version!	Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu18281	NM_001130476.2 Latest version!	Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpst1 ( XM_006504418.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504481.1 Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu36142
Clone ID Related Accession (Same CDS sequence)	XM_006504418.2
Accession Version	XM_006504418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein-tyrosine sulfotransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006504418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGGGA AGCTGAAGCA GAACTTACTC TTGGCGTGTC TGGTGATTAG TTCTGTGACC 
GTGTTTTACC TGGGCCAGCA TGCCATGGAG TGCCATCACC GAATAGAGGA ACGTAGCCAG 
CCAGCCCGAC TGGAGAACCC CAAGGCGACT GTGCGAGCTG GCCTCGACAT CAAAGCCAAC 
AAAACATTCA CCTATCACAA AGATATGCCT TTAATATTCA TCGGGGGTGT GCCTCGGAGC 
GGCACCACAC TCATGAGGGC TATGCTGGAC GCACATCCTG ACATCCGCTG TGGAGAGGAA 
ACCAGGGTCA TCCCTCGAAT CCTGGCCCTG AAGCAGATGT GGTCCCGGTC CAGTAAAGAG 
AAGATCCGCT TGGATGAGGC GGGTGTCACA GATGAAGTGC TAGATTCTGC CATGCAAGCC 
TTCCTTCTGG AGGTCATTGT TAAACATGGG GAGCCGGCAC CTTATTTATG TAACAAAGAT 
CCGTTTGCCC TGAAATCCTT GACTTACCTT GCTAGGTTAT TTCCCAATGC CAAATTTCTC 
CTGATGGTCC GAGATGGCCG GGCGTCAGTA CATTCAATGA TTTCTCGGAA AGTTACTATA 
GCTGGCTTTG ACCTGAACAG CTACCGGGAC TGTCTGACCA AGTGGAACCG GGCCATAGAA 
ACCATGTACA ACCAGTGTAT GGAAGTTGGT TATAAGAAAT GCATGTTGGT TCACTATGAA 
CAGCTCGTCT TACACCCTGA ACGGTGGATG AGAACGCTCT TAAAGTTCCT CCATATTCCA 
TGGAACCATT CCGTTTTGCA CCATGAAGAA ATGATCGGGA AAGCTGGGGG AGTTTCTCTG 
TCAAAGGTGG AAAGATCAAC AGACCAAGTC ATCAAACCCG TCAACGTGGG GGCGCTATCG 
AAGTGGGTTG GGAAGATACC CCCGGACGTC TTACAAGACA TGGCCGTGAT TGCACCCATG 
CTCGCCAAGC TTGGATATGA CCCATACGCC AATCCTCCTA ACTACGGAAA ACCTGACCCC 
AAGATCCTTG AAAACACCAG GAGGACGGAG CAAGTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504481.1
CDS	389..1450
Translation	MVGKLKQNLLLACLVISSVTVFYLGQHAMECHHRIEERSQPARLENPKATVRAGLDIKANKTFTYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEVIVKHGEPAPYLCNKDPFALKSLTYLARLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLHIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKILENTRRTEQVE

Target ORF information:

RefSeq Version	XM_006504418.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504418.2

ORF Insert Sequence:

ATGGTTGGGA AGCTGAAGCA GAACTTACTC TTGGCGTGTC TGGTGATTAG TTCTGTGACC 
GTGTTTTACC TGGGCCAGCA TGCCATGGAG TGCCATCACC GAATAGAGGA ACGTAGCCAG 
CCAGCCCGAC TGGAGAACCC CAAGGCGACT GTGCGAGCTG GCCTCGACAT CAAAGCCAAC 
AAAACATTCA CCTATCACAA AGATATGCCT TTAATATTCA TCGGGGGTGT GCCTCGGAGC 
GGCACCACAC TCATGAGGGC TATGCTGGAC GCACATCCTG ACATCCGCTG TGGAGAGGAA 
ACCAGGGTCA TCCCTCGAAT CCTGGCCCTG AAGCAGATGT GGTCCCGGTC CAGTAAAGAG 
AAGATCCGCT TGGATGAGGC GGGTGTCACA GATGAAGTGC TAGATTCTGC CATGCAAGCC 
TTCCTTCTGG AGGTCATTGT TAAACATGGG GAGCCGGCAC CTTATTTATG TAACAAAGAT 
CCGTTTGCCC TGAAATCCTT GACTTACCTT GCTAGGTTAT TTCCCAATGC CAAATTTCTC 
CTGATGGTCC GAGATGGCCG GGCGTCAGTA CATTCAATGA TTTCTCGGAA AGTTACTATA 
GCTGGCTTTG ACCTGAACAG CTACCGGGAC TGTCTGACCA AGTGGAACCG GGCCATAGAA 
ACCATGTACA ACCAGTGTAT GGAAGTTGGT TATAAGAAAT GCATGTTGGT TCACTATGAA 
CAGCTCGTCT TACACCCTGA ACGGTGGATG AGAACGCTCT TAAAGTTCCT CCATATTCCA 
TGGAACCATT CCGTTTTGCA CCATGAAGAA ATGATCGGGA AAGCTGGGGG AGTTTCTCTG 
TCAAAGGTGG AAAGATCAAC AGACCAAGTC ATCAAACCCG TCAACGTGGG GGCGCTATCG 
AAGTGGGTTG GGAAGATACC CCCGGACGTC TTACAAGACA TGGCCGTGAT TGCACCCATG 
CTCGCCAAGC TTGGATATGA CCCATACGCC AATCCTCCTA ACTACGGAAA ACCTGACCCC 
AAGATCCTTG AAAACACCAG GAGGACGGAG CAAGTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpst1 ( NM_013837.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038865.1 Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18281
Clone ID Related Accession (Same CDS sequence)	NM_013837.2 , NM_001130476.2
Accession Version	NM_013837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	protein-tyrosine sulfotransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF038008.1 and AI183198.1. On Oct 2, 2013 this sequence version replaced NM_013837.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF038008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTGGGA AGCTGAAGCA GAACTTACTC TTGGCGTGTC TGGTGATTAG TTCTGTGACC 
GTGTTTTACC TGGGCCAGCA TGCCATGGAG TGCCATCACC GAATAGAGGA ACGTAGCCAG 
CCAGCCCGAC TGGAGAACCC CAAGGCGACT GTGCGAGCTG GCCTCGACAT CAAAGCCAAC 
AAAACATTCA CCTATCACAA AGATATGCCT TTAATATTCA TCGGGGGTGT GCCTCGGAGC 
GGCACCACAC TCATGAGGGC TATGCTGGAC GCACATCCTG ACATCCGCTG TGGAGAGGAA 
ACCAGGGTCA TCCCTCGAAT CCTGGCCCTG AAGCAGATGT GGTCCCGGTC CAGTAAAGAG 
AAGATCCGCT TGGATGAGGC GGGTGTCACA GATGAAGTGC TAGATTCTGC CATGCAAGCC 
TTCCTTCTGG AGGTCATTGT TAAACATGGG GAGCCGGCAC CTTATTTATG TAACAAAGAT 
CCGTTTGCCC TGAAATCCTT GACTTACCTT GCTAGGTTAT TTCCCAATGC CAAATTTCTC 
CTGATGGTCC GAGATGGCCG GGCGTCAGTA CATTCAATGA TTTCTCGGAA AGTTACTATA 
GCTGGCTTTG ACCTGAACAG CTACCGGGAC TGTCTGACCA AGTGGAACCG GGCCATAGAA 
ACCATGTACA ACCAGTGTAT GGAAGTTGGT TATAAGAAAT GCATGTTGGT TCACTATGAA 
CAGCTCGTCT TACACCCTGA ACGGTGGATG AGAACGCTCT TAAAGTTCCT CCATATTCCA 
TGGAACCATT CCGTTTTGCA CCATGAAGAA ATGATCGGGA AAGCTGGGGG AGTTTCTCTG 
TCAAAGGTGG AAAGATCAAC AGACCAAGTC ATCAAACCCG TCAACGTGGG GGCGCTATCG 
AAGTGGGTTG GGAAGATACC CCCGGACGTC TTACAAGACA TGGCCGTGAT TGCACCCATG 
CTCGCCAAGC TTGGATATGA CCCATACGCC AATCCTCCTA ACTACGGAAA ACCTGACCCC 
AAGATCCTTG AAAACACCAG GAGGGTCTAT AAAGGAGAAT TTCAGCTCCC TGACTTTCTG 
AAAGAAAAAC CCCAGACGGA GCAAGTGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038865.1
CDS	211..1323
Translation	MVGKLKQNLLLACLVISSVTVFYLGQHAMECHHRIEERSQPARLENPKATVRAGLDIKANKTFTYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEVIVKHGEPAPYLCNKDPFALKSLTYLARLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLHIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKILENTRRVYKGEFQLPDFLKEKPQTEQVE

Target ORF information:

RefSeq Version	NM_013837.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013837.2

ORF Insert Sequence:

ATGGTTGGGA AGCTGAAGCA GAACTTACTC TTGGCGTGTC TGGTGATTAG TTCTGTGACC 
GTGTTTTACC TGGGCCAGCA TGCCATGGAG TGCCATCACC GAATAGAGGA ACGTAGCCAG 
CCAGCCCGAC TGGAGAACCC CAAGGCGACT GTGCGAGCTG GCCTCGACAT CAAAGCCAAC 
AAAACATTCA CCTATCACAA AGATATGCCT TTAATATTCA TCGGGGGTGT GCCTCGGAGC 
GGCACCACAC TCATGAGGGC TATGCTGGAC GCACATCCTG ACATCCGCTG TGGAGAGGAA 
ACCAGGGTCA TCCCTCGAAT CCTGGCCCTG AAGCAGATGT GGTCCCGGTC CAGTAAAGAG 
AAGATCCGCT TGGATGAGGC GGGTGTCACA GATGAAGTGC TAGATTCTGC CATGCAAGCC 
TTCCTTCTGG AGGTCATTGT TAAACATGGG GAGCCGGCAC CTTATTTATG TAACAAAGAT 
CCGTTTGCCC TGAAATCCTT GACTTACCTT GCTAGGTTAT TTCCCAATGC CAAATTTCTC 
CTGATGGTCC GAGATGGCCG GGCGTCAGTA CATTCAATGA TTTCTCGGAA AGTTACTATA 
GCTGGCTTTG ACCTGAACAG CTACCGGGAC TGTCTGACCA AGTGGAACCG GGCCATAGAA 
ACCATGTACA ACCAGTGTAT GGAAGTTGGT TATAAGAAAT GCATGTTGGT TCACTATGAA 
CAGCTCGTCT TACACCCTGA ACGGTGGATG AGAACGCTCT TAAAGTTCCT CCATATTCCA 
TGGAACCATT CCGTTTTGCA CCATGAAGAA ATGATCGGGA AAGCTGGGGG AGTTTCTCTG 
TCAAAGGTGG AAAGATCAAC AGACCAAGTC ATCAAACCCG TCAACGTGGG GGCGCTATCG 
AAGTGGGTTG GGAAGATACC CCCGGACGTC TTACAAGACA TGGCCGTGAT TGCACCCATG 
CTCGCCAAGC TTGGATATGA CCCATACGCC AATCCTCCTA ACTACGGAAA ACCTGACCCC 
AAGATCCTTG AAAACACCAG GAGGGTCTAT AAAGGAGAAT TTCAGCTCCC TGACTTTCTG 
AAAGAAAAAC CCCAGACGGA GCAAGTGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpst1 ( NM_001130476.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001123948.1 Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18281
Clone ID Related Accession (Same CDS sequence)	NM_013837.2 , NM_001130476.2
Accession Version	NM_001130476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	protein-tyrosine sulfotransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ046784.1, AK150753.1 and AI183198.1. On Oct 2, 2013 this sequence version replaced NM_001130476.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK150753.1, SRR1660815.216619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTGGGA AGCTGAAGCA GAACTTACTC TTGGCGTGTC TGGTGATTAG TTCTGTGACC 
GTGTTTTACC TGGGCCAGCA TGCCATGGAG TGCCATCACC GAATAGAGGA ACGTAGCCAG 
CCAGCCCGAC TGGAGAACCC CAAGGCGACT GTGCGAGCTG GCCTCGACAT CAAAGCCAAC 
AAAACATTCA CCTATCACAA AGATATGCCT TTAATATTCA TCGGGGGTGT GCCTCGGAGC 
GGCACCACAC TCATGAGGGC TATGCTGGAC GCACATCCTG ACATCCGCTG TGGAGAGGAA 
ACCAGGGTCA TCCCTCGAAT CCTGGCCCTG AAGCAGATGT GGTCCCGGTC CAGTAAAGAG 
AAGATCCGCT TGGATGAGGC GGGTGTCACA GATGAAGTGC TAGATTCTGC CATGCAAGCC 
TTCCTTCTGG AGGTCATTGT TAAACATGGG GAGCCGGCAC CTTATTTATG TAACAAAGAT 
CCGTTTGCCC TGAAATCCTT GACTTACCTT GCTAGGTTAT TTCCCAATGC CAAATTTCTC 
CTGATGGTCC GAGATGGCCG GGCGTCAGTA CATTCAATGA TTTCTCGGAA AGTTACTATA 
GCTGGCTTTG ACCTGAACAG CTACCGGGAC TGTCTGACCA AGTGGAACCG GGCCATAGAA 
ACCATGTACA ACCAGTGTAT GGAAGTTGGT TATAAGAAAT GCATGTTGGT TCACTATGAA 
CAGCTCGTCT TACACCCTGA ACGGTGGATG AGAACGCTCT TAAAGTTCCT CCATATTCCA 
TGGAACCATT CCGTTTTGCA CCATGAAGAA ATGATCGGGA AAGCTGGGGG AGTTTCTCTG 
TCAAAGGTGG AAAGATCAAC AGACCAAGTC ATCAAACCCG TCAACGTGGG GGCGCTATCG 
AAGTGGGTTG GGAAGATACC CCCGGACGTC TTACAAGACA TGGCCGTGAT TGCACCCATG 
CTCGCCAAGC TTGGATATGA CCCATACGCC AATCCTCCTA ACTACGGAAA ACCTGACCCC 
AAGATCCTTG AAAACACCAG GAGGGTCTAT AAAGGAGAAT TTCAGCTCCC TGACTTTCTG 
AAAGAAAAAC CCCAGACGGA GCAAGTGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123948.1
CDS	394..1506
Translation	MVGKLKQNLLLACLVISSVTVFYLGQHAMECHHRIEERSQPARLENPKATVRAGLDIKANKTFTYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEVIVKHGEPAPYLCNKDPFALKSLTYLARLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLHIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKILENTRRVYKGEFQLPDFLKEKPQTEQVE

Target ORF information:

RefSeq Version	NM_001130476.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein-tyrosine sulfotransferase 1 (Tpst1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130476.2

ORF Insert Sequence:

ATGGTTGGGA AGCTGAAGCA GAACTTACTC TTGGCGTGTC TGGTGATTAG TTCTGTGACC 
GTGTTTTACC TGGGCCAGCA TGCCATGGAG TGCCATCACC GAATAGAGGA ACGTAGCCAG 
CCAGCCCGAC TGGAGAACCC CAAGGCGACT GTGCGAGCTG GCCTCGACAT CAAAGCCAAC 
AAAACATTCA CCTATCACAA AGATATGCCT TTAATATTCA TCGGGGGTGT GCCTCGGAGC 
GGCACCACAC TCATGAGGGC TATGCTGGAC GCACATCCTG ACATCCGCTG TGGAGAGGAA 
ACCAGGGTCA TCCCTCGAAT CCTGGCCCTG AAGCAGATGT GGTCCCGGTC CAGTAAAGAG 
AAGATCCGCT TGGATGAGGC GGGTGTCACA GATGAAGTGC TAGATTCTGC CATGCAAGCC 
TTCCTTCTGG AGGTCATTGT TAAACATGGG GAGCCGGCAC CTTATTTATG TAACAAAGAT 
CCGTTTGCCC TGAAATCCTT GACTTACCTT GCTAGGTTAT TTCCCAATGC CAAATTTCTC 
CTGATGGTCC GAGATGGCCG GGCGTCAGTA CATTCAATGA TTTCTCGGAA AGTTACTATA 
GCTGGCTTTG ACCTGAACAG CTACCGGGAC TGTCTGACCA AGTGGAACCG GGCCATAGAA 
ACCATGTACA ACCAGTGTAT GGAAGTTGGT TATAAGAAAT GCATGTTGGT TCACTATGAA 
CAGCTCGTCT TACACCCTGA ACGGTGGATG AGAACGCTCT TAAAGTTCCT CCATATTCCA 
TGGAACCATT CCGTTTTGCA CCATGAAGAA ATGATCGGGA AAGCTGGGGG AGTTTCTCTG 
TCAAAGGTGG AAAGATCAAC AGACCAAGTC ATCAAACCCG TCAACGTGGG GGCGCTATCG 
AAGTGGGTTG GGAAGATACC CCCGGACGTC TTACAAGACA TGGCCGTGAT TGCACCCATG 
CTCGCCAAGC TTGGATATGA CCCATACGCC AATCCTCCTA ACTACGGAAA ACCTGACCCC 
AAGATCCTTG AAAACACCAG GAGGGTCTAT AAAGGAGAAT TTCAGCTCCC TGACTTTCTG 
AAAGAAAAAC CCCAGACGGA GCAAGTGGAG TAA