Top2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top2a
Entrez Gene ID	21973
Full Name	topoisomerase (DNA) II alpha
Synonyms	Top-2
General protein information	Preferred Names topoisomerase (DNA) II alpha Names DNA topoisomerase 2-alpha DNA Topoisomerase II alpha DNA topoisomerase II, alpha isozyme
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 D\|11 62.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533155.2	XP_006533218.1	DNA topoisomerase 2-alpha isoform X2
XM_006533154.3	XP_006533217.1	DNA topoisomerase 2-alpha isoform X2
XM_006533155.3	XP_006533218.1	DNA topoisomerase 2-alpha isoform X2
NM_011623.2	NP_035753.2	DNA topoisomerase 2-alpha
XM_006533154.2	XP_006533217.1	DNA topoisomerase 2-alpha isoform X2
XM_006533153.3	XP_006533216.1	DNA topoisomerase 2-alpha isoform X1
XM_006533153.2	XP_006533216.1	DNA topoisomerase 2-alpha isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology
Bibliography

Related articles in PubMed

Defects in chondrocyte maturation and secondary ossification in mouse knee joint epiphyses due to Snorc deficiency.
Heinonen J, Zhang FP, Surmann-Schmitt C, Honkala S, Stock M, Poutanen M, S??m?nen AM
Osteoarthritis and cartilage25(7)1132-1142(2017 Jul)

SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.
Hwang G, Sun F, O'Brien M, Eppig JJ, Handel MA, Jordan PW
Development (Cambridge, England)144(9)1648-1660(2017 05)

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Naiman N, Fujioka K, Fujino M, Valerius MT, Potter SS, McMahon AP, Kobayashi A
Developmental cell41(4)349-365.e3(2017 05)

TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin.
Miller EL, Hargreaves DC, Kadoch C, Chang CY, Calarco JP, Hodges C, Buenrostro JD, Cui K, Greenleaf WJ, Zhao K, Crabtree GR
Nature structural & molecular biology24(4)344-352(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polyamide functionalisation at the N1-position offers a design strategy to improve drug-like properties. Dicationic HxIP* 3 increased topo IIalpha expression and chemosensitivity to topo II-targeting agents.
Title: Modulation of topoisomerase II? expression and chemosensitivity through targeted inhibition of NF-Y:DNA binding by a diamino p-anisyl-benzimidazole (Hx) polyamide.

TOP2 and BAF cooperate to recruit pluripotency factors, which explains some of the instructive roles of BAF complexes.
Title: TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin.

Topo IIalpha plays an important role in adipogenesis.
Title: DNA Topoisomerase II? contributes to the early steps of adipogenesis in 3T3-L1 cells.

Deletion or deficiency of PTEN leads to down regulation of TOP2A, dysfunction of the decatenation checkpoint and incomplete DNA decatenation in G2 and M phases.
Title: PTEN stabilizes TOP2A and regulates the DNA decatenation.

Inhibition of DNA topoisomerase II selectively reduces the threat of tumorigenicity
Title: Inhibition of DNA topoisomerase II selectively reduces the threat of tumorigenicity following induced pluripotent stem cell-based myocardial therapy.

The following Top2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20097	XM_006533153.3 Latest version!	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu46211	XM_006533154.3 Latest version!	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu46211	XM_006533155.3 Latest version!	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu20097	NM_011623.2 Latest version!	Mus musculus topoisomerase (DNA) II alpha (Top2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu46211	XM_006533155.2	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu46211	XM_006533154.2	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu20097	XM_006533153.2	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top2a ( XM_006533153.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533216.1 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20097
Clone ID Related Accession (Same CDS sequence)	NM_011623.2 , XM_006533153.3 , XM_006533153.2
Accession Version	XM_006533153.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533153.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT 
GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT 
GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG 
TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT 
TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA 
GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC 
ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG 
TCCGATGACG ATGACGACCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533216.1
CDS	134..4720
Translation	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Target ORF information:

RefSeq Version	XM_006533153.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533153.3

ORF Insert Sequence:

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT 
GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT 
GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG 
TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT 
TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA 
GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC 
ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG 
TCCGATGACG ATGACGACCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top2a ( XM_006533154.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533217.1 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46211
Clone ID Related Accession (Same CDS sequence)	XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
Accession Version	XM_006533154.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533154.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533217.1
CDS	134..4717
Translation	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Target ORF information:

RefSeq Version	XM_006533154.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533154.3

ORF Insert Sequence:

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top2a ( XM_006533155.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533218.1 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu46211
Clone ID Related Accession (Same CDS sequence)	XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
Accession Version	XM_006533155.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533155.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533218.1
CDS	134..4717
Translation	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Target ORF information:

RefSeq Version	XM_006533155.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533155.3

ORF Insert Sequence:

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top2a ( NM_011623.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035753.2 Mus musculus topoisomerase (DNA) II alpha (Top2a), mRNA.

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CloneID	OMu20097
Clone ID Related Accession (Same CDS sequence)	NM_011623.2 , XM_006533153.3 , XM_006533153.2
Accession Version	NM_011623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591067.35. On Jul 26, 2007 this sequence version replaced NM_011623.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12513.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT 
GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT 
GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG 
TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT 
TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA 
GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC 
ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG 
TCCGATGACG ATGACGACCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035753.2
CDS	124..4710
Translation	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Target ORF information:

RefSeq Version	NM_011623.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II alpha (Top2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011623.2

ORF Insert Sequence:

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT 
GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT 
GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG 
TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT 
TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA 
GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC 
ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG 
TCCGATGACG ATGACGACCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top2a ( XM_006533153.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533216.1 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.

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CloneID	OMu20097
Clone ID Related Accession (Same CDS sequence)	NM_011623.2 , XM_006533153.3 , XM_006533153.2
Accession Version	XM_006533153.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT 
GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT 
GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG 
TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT 
TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA 
GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC 
ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG 
TCCGATGACG ATGACGACCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533216.1
CDS	140..4726
Translation	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Target ORF information:

RefSeq Version	XM_006533153.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533153.2

ORF Insert Sequence:

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT 
GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT 
GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG 
TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT 
TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA 
GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC 
ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG 
TCCGATGACG ATGACGACCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top2a ( XM_006533155.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533218.1 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu46211
Clone ID Related Accession (Same CDS sequence)	XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
Accession Version	XM_006533155.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533218.1
CDS	140..4723
Translation	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Target ORF information:

RefSeq Version	XM_006533155.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533155.2

ORF Insert Sequence:

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top2a ( XM_006533154.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533217.1 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46211
Clone ID Related Accession (Same CDS sequence)	XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
Accession Version	XM_006533154.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533217.1
CDS	140..4723
Translation	MELSPLQPVNENMLMNKKKNEDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVKKGRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDLDSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVDLESDVKDSVPASPGVPAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKAKNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEESDDDDDLF

Target ORF information:

RefSeq Version	XM_006533154.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533154.2

ORF Insert Sequence:

ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT 
GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA 
TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT 
CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA 
GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT 
GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT 
GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA 
AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT 
CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA 
ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA 
ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG 
GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG 
AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG 
ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG 
ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT 
GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC 
CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT 
GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG 
GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT 
GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC 
TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT 
CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG 
CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC 
AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG 
ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA 
AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT 
GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA 
AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT 
GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG 
GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT 
TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA 
GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG 
ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG 
AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT 
AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA 
GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC 
ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC 
TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT 
GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA 
CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT 
GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA 
CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT 
ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG 
AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG 
CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC 
TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT 
GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC 
AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC 
TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA 
GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG 
ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG 
CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA 
TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC 
AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG 
AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA 
GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA 
AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA 
GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT 
GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT 
GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC 
AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG 
GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT 
AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA 
CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT 
TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA 
GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA 
AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT 
GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG 
ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC 
GATGACGATG ACGACCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defects in chondrocyte maturation and secondary ossification in mouse knee joint epiphyses due to Snorc deficiency.
Osteoarthritis and cartilage25(7)1132-1142(2017 Jul)
Heinonen J,Zhang FP,Surmann-Schmitt C,Honkala S,Stock M,Poutanen M,S??m?nen AM

SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.
Development (Cambridge, England)144(9)1648-1660(2017 05)
Hwang G,Sun F,O'Brien M,Eppig JJ,Handel MA,Jordan PW

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Developmental cell41(4)349-365.e3(2017 05)
Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin.
Nature structural & molecular biology24(4)344-352(2017 Apr)
Miller EL,Hargreaves DC,Kadoch C,Chang CY,Calarco JP,Hodges C,Buenrostro JD,Cui K,Greenleaf WJ,Zhao K,Crabtree GR

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Top2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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