Tie1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tie1
Entrez Gene ID	21846
Full Name	tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Synonyms	D430008P04Rik,TIE,tie-1
General protein information	Preferred Names tyrosine kinase with immunoglobulin-like and EGF-like domains 1 Names tyrosine-protein kinase receptor Tie-1 tyrosine kinase receptor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D2.1\|4 54.67 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011587.2	NP_035717.2	tyrosine-protein kinase receptor Tie-1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TIE1	NP_776390.1
Rattus norvegicus (Norway rat)	Tie1	NP_445997.1
Gallus gallus (chicken)	TIE1	XP_004936746.1
Danio rerio (zebrafish)	tie1	XP_001334673.5
Pan troglodytes (chimpanzee)	TIE1	XP_001173341.1
Canis lupus familiaris (dog)	TIE1	XP_539652.3
Mus musculus (house mouse)	Tie1	NP_035717.2
Xenopus tropicalis (tropical clawed frog)	tie1	XP_002931650.2
Homo sapiens (human)	TIE1	NP_005415.1

Gene Ontology
Bibliography

Related articles in PubMed

Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis.
La Porta S, Roth L, Singhal M, Mogler C, Spegg C, Schieb B, Qu X, Adams RH, Baldwin HS, Savant S, Augustin HG
The Journal of clinical investigation(2018 Jan)

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Wang Y, Wu B, Lu P, Zhang D, Wu B, Varshney S, Del Monte-Nieto G, Zhuang Z, Charafeddine R, Kramer AH, Sibinga NE, Frangogiannis NG, Kitsis RN, Adams RH, Alitalo K, Sharp DJ, Harvey RP, Stanley P, Zhou B
Nature communications8(1)578(2017 09)

Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.
Sleigh JN, G?mez-Mart?n A, Wei N, Bai G, Yang XL, Schiavo G
Scientific reports7(1)9216(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data support an interactive model of Tie1 and Tie2 function, in which dynamically regulated Tie1 versus Tie2 expression determines the net positive or negative effect of Tie1 on Tie2 signaling.
Title: The Orphan Receptor Tie1 Controls Angiogenesis and Vascular Remodeling by Differentially Regulating Tie2 in Tip and Stalk Cells.

Data indicate that receptor tyrosine kinase Tie1 deletion resulted in lack of lymphatic valves and collecting vessel defects.
Title: Tie1 is required for lymphatic valve and collecting vessel development.

In contrast to the important role of Tie2 in the regulation of blood vascular development, Tie1 is crucial in the process of lymphatic remodeling and maturation, which is independent of Tie2.
Title: Genetic dissection of tie pathway in mouse lymphatic maturation and valve development.

Tie1 regulates tumor angiogenesis, postnatal sprouting angiogenesis, and endothelial cell survival, which are controlled by VEGF, Angpt, and Notch signals
Title: Tie1 deletion inhibits tumor growth and improves angiopoietin antagonist therapy.

Shear stress conditions that modulate atherogenic events also regulate Tie1 expression. Therefore, Tie1 may play a novel proinflammatory role in atherosclerosis.
Title: Tie1 attenuation reduces murine atherosclerosis in a dose-dependent and shear stress-specific manner.

The following Tie1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tie1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17791	NM_011587.2 Latest version!	Mus musculus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$664.30 $949.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tie1 ( NM_011587.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035717.2 Mus musculus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), mRNA.

Price & Availability↑

CloneID	OMu17791
Clone ID Related Accession (Same CDS sequence)	NM_011587.2
Accession Version	NM_011587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase receptor Tie-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB841681.1, AK052413.1, AK052192.1 and CR518034.1. On Apr 10, 2007 this sequence version replaced NM_011587.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X80764.1, SRR1660821.62762.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTCTGGT GGGGATCCTC TTTGCTGCTC CCCACTCTTT TCTTGGCCTC TCATGTTGGT 
GCGTCTGTTG ACTTAACATT GCTGGCCAAC CTGCGCATCA CAGACCCCCA GCGTTTCTTC 
CTGACCTGCG TGTCTGGTGA GGCCGGAGCA GGGAGGAGCT CGGACCCGCC CCTGCTGCTG 
GAGAAGGATG ATCGCATCGT GCGCACCTTC CCGCCTGGGC AGCCCCTGTA CCTGGCACGC 
AACGGTTCTC ACCAGGTCAC GCTGAGGGGC TTCTCCAAGC CCTCGGATTT GGTAGGCGTC 
TTCTCCTGTG TGGGTGGTGC TGGCGCGCGG CGCACTCGAG TTCTCTATGT GCACAACAGC 
CCAGGGGCAC ACCTGTTTCC AGACAAGGTC ACACACACGG TGAACAAAGG TGACACTGCT 
GTGCTTTCTG CCCATGTGCA CAAGGAAAAG CAGACTGACG TGATCTGGAA GAACAACGGA 
TCCTACTTCA ATACCCTAGA CTGGCAAGAG GCCGATGACG GGCGTTTTCA ACTGCAGCTC 
CAAAATGTAC AGCCACCATC AAGTGGCATC TACAGTGCCA CCTACCTAGA GGCCAGCCCC 
CTGGGCAGTG CCTTCTTTCG GCTCATCGTG CGAGGCTGTG GGGCTGGACG CTGGGGGCCA 
GGATGTGTCA AGGATTGCCC AGGCTGCCTG CATGGAGGTG TCTGTCATGA CCATGATGGC 
GAATGTGTGT GTCCCCCAGG ATTCACTGGC ACCCGCTGTG AGCAGGCCTG CAGGGAGGGT 
CGTTTTGGAC AGAGCTGTCA GGAACAGTGC CCAGGCACAG CAGGTTGTAG AGGTCTCACC 
TTCTGCCTCC CGGATCCCTA TGGCTGTTCC TGTGGATCTG GCTGGAGAGG AAGCCAGTGC 
CAGGAAGCAT GTGCACCTGG TCATTTTGGG GCTGATTGTC GCCTCCAGTG CCAGTGTCAA 
AATGGTGGCA CTTGTGACCG GTTCAGCGGC TGCGTCTGCC CCTCTGGGTG GCATGGAGTC 
CACTGTGAAA AGTCAGACCG GATCCCCCAG ATCCTCAGTA TGGCCACAGA GGTGGAGTTC 
AACATAGGGA CGATGCCCCG GATCAACTGT GCAGCTGCCG GGAATCCCTT CCCAGTGCGG 
GGCAGCATGA AACTTCGCAA GCCAGATGGC ACCATGCTTT TGTCTACCAA AGTCATTGTG 
GAGCCAGACA GGACCACAGC AGAGTTTGAA GTGCCCAGTT TGACTCTTGG GGACAGTGGG 
TTCTGGGAAT GCCGCGTATC GACTTCTGGT GGCCAAGACA GCCGGCGCTT CAAGGTCAAT 
GTCAAAGTAC CCCCAGTGCC TTTGACTGCA CCTCGACTCC TGGCCAAGCA GAGCCGTCAG 
CTTGTGGTCT CCCCACTGGT CTCCTTTAGT GGGGATGGAC CCATCTCCTC TGTCCGCCTG 
CACTACCGGC CCCAGGACAG CACGATTGCC TGGTCTGCCA TTGTGGTGGA TCCCAGTGAG 
AATGTGACAT TAATGAACCT GAAGCCAAAG ACAGGATACA ATGTCCGAGT GCAGCTGAGC 
CGGCCAGGGG AAGGAGGAGA AGGAGGCTGG GGACCTTCTG CCCTTATGAC TACTGACTGT 
CCTGAGCCTT TGCTGCAGCC CTGGCTAGAG AGCTGGCACG TGGAGGGTCC TGACAGGCTC 
CGTGTGAGCT GGTCTCTACC CTCGGTGCCA CTGTCCGGTG ATGGTTTCCT GCTGCGCCTG 
TGGGATGGGG CCCGGGGACA GGAGAGGCGG GAGAACATCT CATTCCCCCA GGCCCGCACT 
GCCCTCCTGA CTGGACTCAC GCCGGGCACC CACTACCAGC TGGATGTGCG ACTGTATCAC 
TGTACCCTCC TGGGCCCTGC CTCACCCCCT GCGCATGTGC ACCTGCCCCC CAGCGGGCCT 
CCAGCTCCCC GCCACCTCCA TGCCCAGGCC CTCTCAGACT CTGAGATTCA ACTCATGTGG 
CAGCATCCCG AGGCTCCGTC TGGGCCTATA TCCAAGTACA TCGTGGAGAT TCAGGTGGCC 
GGGGGCTCAG GAGACCCACA GTGGATGGAT GTAGACAGGC CCGAGGAGAC AAGCATCATT 
GTTCGTGGCC TCAATGCTAG CACACGATAT CTTTTCCGTG TGCGGGCCAG TGTGCAGGGT 
CTTGGCGACT GGAGCAATAC AGTAGAAGAG GCCACTCTGG GCAATGGGCT GCAGAGTGAG 
GGCCCAGTCC GAGAAAGCCG GGCAGCTGAA GAAGGCCTGG ATCAGCAGCT GGTCCTGGCT 
GTGGTAGGTT CCGTCTCTGC CACCTGCCTC ACCATCCTGG CTGCCCTTTT AGCCTTGGTG 
TGTATCCGAA GAAGCTGCCT ACATCGGAGA CGCACCTTCA CTTACCAGTC AGGATCGGGT 
GAAGAGACCA TCCTGCAGTT CAGCTCAGGG ACCTTGACCT TGACCCGGCG ACCGAAGCCA 
CAGCCTGAGC CCTTGAGTTA CCCCGTGCTG GAGTGGGAGG ACATCACCTT TGAGGACCTC 
ATAGGGGAGG GCAACTTTGG CCAAGTGATC CGGGCCATGA TCAAGAAGGA CGGGCTCAAG 
ATGAACGCAG CCATCAAGAT GCTAAAAGAG TATGCGTCTG AAAATGACCA TCGAGACTTT 
GCAGGTGAAC TAGAAGTTCT GTGCAAACTA GGACACCACC CCAATATTAT CAACCTCTTG 
GGGGCCTGTG AGAACCGAGG CTACTTGTAT ATTGCTATCG AATACGCCCC CTATGGAAAC 
CTACTGGACT TCCTGAGAAA GAGCAGGGTC CTGGAGACTG ATCCAGCTTT TGCTCGAGAG 
CATGGGACGG CCTCCACACT CAGCTCTCGG CAGCTGCTGC GCTTTGCCAG TGATGCAGCC 
AACGGCATGC AGTACCTTAG TGAGAAGCAG TTCATTCACA GGGACCTGGC TGCCCGAAAT 
GTGCTGGTCG GAGAGAACCT GGCCTCCAAG ATTGCAGACT TTGGCCTTTC TCGAGGGGAG 
GAGGTGTATG TGAAGAAGAC AATGGGCCGT CTCCCTGTGC GTTGGATGGC CATTGAGTCC 
CTCAACTACA GCGTCTATAC CACCAAGAGT GATGTCTGGT CCTTTGGGGT CCTCCTCTGG 
GAGATAGTGA GCCTTGGAGG CACGCCCTAC TGTGGCATGA CCTGTGCTGA GCTCTACGAG 
AAGCTGCCTC AAGGCTATCG CATGGAGCAG CCTCGAAACT GCGATGACGA AGTGTACGAG 
CTGATGCGGC AGTGCTGGCG GGATCGGCCC TATGAGCGCC CCCCCTTTGC TCAGATCGCA 
CTACAGTTGG GCCGCATGCT GGAAGCCAGG AAGGCCTACG TGAACATGTC GCTGTTTGAG 
AACTTCACCT ATGCGGGCAT CGATGCCACA GCTGAGGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035717.2
CDS	145..3549
Translation	MVWWGSSLLLPTLFLASHVGASVDLTLLANLRITDPQRFFLTCVSGEAGAGRSSDPPLLLEKDDRIVRTFPPGQPLYLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVLYVHNSPGAHLFPDKVTHTVNKGDTAVLSAHVHKEKQTDVIWKNNGSYFNTLDWQEADDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILSMATEVEFNIGTMPRINCAAAGNPFPVRGSMKLRKPDGTMLLSTKVIVEPDRTTAEFEVPSLTLGDSGFWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFSGDGPISSVRLHYRPQDSTIAWSAIVVDPSENVTLMNLKPKTGYNVRVQLSRPGEGGEGGWGPSALMTTDCPEPLLQPWLESWHVEGPDRLRVSWSLPSVPLSGDGFLLRLWDGARGQERRENISFPQARTALLTGLTPGTHYQLDVRLYHCTLLGPASPPAHVHLPPSGPPAPRHLHAQALSDSEIQLMWQHPEAPSGPISKYIVEIQVAGGSGDPQWMDVDRPEETSIIVRGLNASTRYLFRVRASVQGLGDWSNTVEEATLGNGLQSEGPVRESRAAEEGLDQQLVLAVVGSVSATCLTILAALLALVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	NM_011587.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011587.2

ORF Insert Sequence:

ATGGTCTGGT GGGGATCCTC TTTGCTGCTC CCCACTCTTT TCTTGGCCTC TCATGTTGGT 
GCGTCTGTTG ACTTAACATT GCTGGCCAAC CTGCGCATCA CAGACCCCCA GCGTTTCTTC 
CTGACCTGCG TGTCTGGTGA GGCCGGAGCA GGGAGGAGCT CGGACCCGCC CCTGCTGCTG 
GAGAAGGATG ATCGCATCGT GCGCACCTTC CCGCCTGGGC AGCCCCTGTA CCTGGCACGC 
AACGGTTCTC ACCAGGTCAC GCTGAGGGGC TTCTCCAAGC CCTCGGATTT GGTAGGCGTC 
TTCTCCTGTG TGGGTGGTGC TGGCGCGCGG CGCACTCGAG TTCTCTATGT GCACAACAGC 
CCAGGGGCAC ACCTGTTTCC AGACAAGGTC ACACACACGG TGAACAAAGG TGACACTGCT 
GTGCTTTCTG CCCATGTGCA CAAGGAAAAG CAGACTGACG TGATCTGGAA GAACAACGGA 
TCCTACTTCA ATACCCTAGA CTGGCAAGAG GCCGATGACG GGCGTTTTCA ACTGCAGCTC 
CAAAATGTAC AGCCACCATC AAGTGGCATC TACAGTGCCA CCTACCTAGA GGCCAGCCCC 
CTGGGCAGTG CCTTCTTTCG GCTCATCGTG CGAGGCTGTG GGGCTGGACG CTGGGGGCCA 
GGATGTGTCA AGGATTGCCC AGGCTGCCTG CATGGAGGTG TCTGTCATGA CCATGATGGC 
GAATGTGTGT GTCCCCCAGG ATTCACTGGC ACCCGCTGTG AGCAGGCCTG CAGGGAGGGT 
CGTTTTGGAC AGAGCTGTCA GGAACAGTGC CCAGGCACAG CAGGTTGTAG AGGTCTCACC 
TTCTGCCTCC CGGATCCCTA TGGCTGTTCC TGTGGATCTG GCTGGAGAGG AAGCCAGTGC 
CAGGAAGCAT GTGCACCTGG TCATTTTGGG GCTGATTGTC GCCTCCAGTG CCAGTGTCAA 
AATGGTGGCA CTTGTGACCG GTTCAGCGGC TGCGTCTGCC CCTCTGGGTG GCATGGAGTC 
CACTGTGAAA AGTCAGACCG GATCCCCCAG ATCCTCAGTA TGGCCACAGA GGTGGAGTTC 
AACATAGGGA CGATGCCCCG GATCAACTGT GCAGCTGCCG GGAATCCCTT CCCAGTGCGG 
GGCAGCATGA AACTTCGCAA GCCAGATGGC ACCATGCTTT TGTCTACCAA AGTCATTGTG 
GAGCCAGACA GGACCACAGC AGAGTTTGAA GTGCCCAGTT TGACTCTTGG GGACAGTGGG 
TTCTGGGAAT GCCGCGTATC GACTTCTGGT GGCCAAGACA GCCGGCGCTT CAAGGTCAAT 
GTCAAAGTAC CCCCAGTGCC TTTGACTGCA CCTCGACTCC TGGCCAAGCA GAGCCGTCAG 
CTTGTGGTCT CCCCACTGGT CTCCTTTAGT GGGGATGGAC CCATCTCCTC TGTCCGCCTG 
CACTACCGGC CCCAGGACAG CACGATTGCC TGGTCTGCCA TTGTGGTGGA TCCCAGTGAG 
AATGTGACAT TAATGAACCT GAAGCCAAAG ACAGGATACA ATGTCCGAGT GCAGCTGAGC 
CGGCCAGGGG AAGGAGGAGA AGGAGGCTGG GGACCTTCTG CCCTTATGAC TACTGACTGT 
CCTGAGCCTT TGCTGCAGCC CTGGCTAGAG AGCTGGCACG TGGAGGGTCC TGACAGGCTC 
CGTGTGAGCT GGTCTCTACC CTCGGTGCCA CTGTCCGGTG ATGGTTTCCT GCTGCGCCTG 
TGGGATGGGG CCCGGGGACA GGAGAGGCGG GAGAACATCT CATTCCCCCA GGCCCGCACT 
GCCCTCCTGA CTGGACTCAC GCCGGGCACC CACTACCAGC TGGATGTGCG ACTGTATCAC 
TGTACCCTCC TGGGCCCTGC CTCACCCCCT GCGCATGTGC ACCTGCCCCC CAGCGGGCCT 
CCAGCTCCCC GCCACCTCCA TGCCCAGGCC CTCTCAGACT CTGAGATTCA ACTCATGTGG 
CAGCATCCCG AGGCTCCGTC TGGGCCTATA TCCAAGTACA TCGTGGAGAT TCAGGTGGCC 
GGGGGCTCAG GAGACCCACA GTGGATGGAT GTAGACAGGC CCGAGGAGAC AAGCATCATT 
GTTCGTGGCC TCAATGCTAG CACACGATAT CTTTTCCGTG TGCGGGCCAG TGTGCAGGGT 
CTTGGCGACT GGAGCAATAC AGTAGAAGAG GCCACTCTGG GCAATGGGCT GCAGAGTGAG 
GGCCCAGTCC GAGAAAGCCG GGCAGCTGAA GAAGGCCTGG ATCAGCAGCT GGTCCTGGCT 
GTGGTAGGTT CCGTCTCTGC CACCTGCCTC ACCATCCTGG CTGCCCTTTT AGCCTTGGTG 
TGTATCCGAA GAAGCTGCCT ACATCGGAGA CGCACCTTCA CTTACCAGTC AGGATCGGGT 
GAAGAGACCA TCCTGCAGTT CAGCTCAGGG ACCTTGACCT TGACCCGGCG ACCGAAGCCA 
CAGCCTGAGC CCTTGAGTTA CCCCGTGCTG GAGTGGGAGG ACATCACCTT TGAGGACCTC 
ATAGGGGAGG GCAACTTTGG CCAAGTGATC CGGGCCATGA TCAAGAAGGA CGGGCTCAAG 
ATGAACGCAG CCATCAAGAT GCTAAAAGAG TATGCGTCTG AAAATGACCA TCGAGACTTT 
GCAGGTGAAC TAGAAGTTCT GTGCAAACTA GGACACCACC CCAATATTAT CAACCTCTTG 
GGGGCCTGTG AGAACCGAGG CTACTTGTAT ATTGCTATCG AATACGCCCC CTATGGAAAC 
CTACTGGACT TCCTGAGAAA GAGCAGGGTC CTGGAGACTG ATCCAGCTTT TGCTCGAGAG 
CATGGGACGG CCTCCACACT CAGCTCTCGG CAGCTGCTGC GCTTTGCCAG TGATGCAGCC 
AACGGCATGC AGTACCTTAG TGAGAAGCAG TTCATTCACA GGGACCTGGC TGCCCGAAAT 
GTGCTGGTCG GAGAGAACCT GGCCTCCAAG ATTGCAGACT TTGGCCTTTC TCGAGGGGAG 
GAGGTGTATG TGAAGAAGAC AATGGGCCGT CTCCCTGTGC GTTGGATGGC CATTGAGTCC 
CTCAACTACA GCGTCTATAC CACCAAGAGT GATGTCTGGT CCTTTGGGGT CCTCCTCTGG 
GAGATAGTGA GCCTTGGAGG CACGCCCTAC TGTGGCATGA CCTGTGCTGA GCTCTACGAG 
AAGCTGCCTC AAGGCTATCG CATGGAGCAG CCTCGAAACT GCGATGACGA AGTGTACGAG 
CTGATGCGGC AGTGCTGGCG GGATCGGCCC TATGAGCGCC CCCCCTTTGC TCAGATCGCA 
CTACAGTTGG GCCGCATGCT GGAAGCCAGG AAGGCCTACG TGAACATGTC GCTGTTTGAG 
AACTTCACCT ATGCGGGCAT CGATGCCACA GCTGAGGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis.
The Journal of clinical investigation(2018 Jan)
La Porta S,Roth L,Singhal M,Mogler C,Spegg C,Schieb B,Qu X,Adams RH,Baldwin HS,Savant S,Augustin HG

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Nature communications8(1)578(2017 09)
Wang Y,Wu B,Lu P,Zhang D,Wu B,Varshney S,Del Monte-Nieto G,Zhuang Z,Charafeddine R,Kramer AH,Sibinga NE,Frangogiannis NG,Kitsis RN,Adams RH,Alitalo K,Sharp DJ,Harvey RP,Stanley P,Zhou B

Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.
Scientific reports7(1)9216(2017 Aug)
Sleigh JN,G?mez-Mart?n A,Wei N,Bai G,Yang XL,Schiavo G