Nploc4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nploc4
Entrez Gene ID	217365
Full Name	NPL4 homolog, ubiquitin recognition factor
Synonyms	AK129375,Npl4,mKIAA1499
General protein information	Preferred Names NPL4 homolog, ubiquitin recognition factor Names nuclear protein localization protein 4 homolog nuclear protein localization 4 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195023.1	NP_001181952.1	nuclear protein localization protein 4 homolog isoform A
NM_199469.2	NP_955763.1	nuclear protein localization protein 4 homolog isoform B
XM_006533134.2	XP_006533197.1	nuclear protein localization protein 4 homolog isoform X1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	NPL4	NP_009729.1
Kluyveromyces lactis	KLLA0E24575g	XP_455058.1
Eremothecium gossypii	AGOS_AGR211W	NP_986877.1
Magnaporthe oryzae (rice blast fungus)	MGG_04413	XP_003713511.1
	LOC713927	XP_001111288.1
Rattus norvegicus (Norway rat)	Nploc4	NP_542144.1
Danio rerio (zebrafish)	nploc4	NP_956101.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001986	XP_550827.4
Xenopus tropicalis (tropical clawed frog)	nploc4	NP_001135712.1
Pan troglodytes (chimpanzee)	NPLOC4	XP_003315818.1
Canis lupus familiaris (dog)	NPLOC4	XP_540482.2
Drosophila melanogaster (fruit fly)	Npl4	NP_001163731.1
Homo sapiens (human)	NPLOC4	NP_060391.2
Mus musculus (house mouse)	Nploc4	NP_001181952.1
Gallus gallus (chicken)	NPLOC4	XP_415704.3
Caenorhabditis elegans (roundworm)	npl-4.2	NP_495093.1
Caenorhabditis elegans (roundworm)	npl-4.1	NP_495096.1
Bos taurus (cattle)	NPLOC4	NP_001179118.1
Schizosaccharomyces pombe (fission yeast)	npl4	NP_595883.2
Neurospora crassa	NCU02680	XP_965453.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential Involvement of the Npl4 Zinc Finger Domains of SHARPIN and HOIL-1L in Linear Ubiquitin Chain Assembly Complex-Mediated Cell Death Protection.
Shimizu S, Fujita H, Sasaki Y, Tsuruyama T, Fukuda K, Iwai K
Molecular and cellular biology36(10)1569-83(2016 05)

The Transitional Endoplasmic Reticulum ATPase p97 Regulates the Alternative Nuclear Factor NF-?B Signaling via Partial Degradation of the NF-?B Subunit p100.
Zhang Z, Wang Y, Li C, Shi Z, Hao Q, Wang W, Song X, Zhao Y, Jiao S, Zhou Z
The Journal of biological chemistry290(32)19558-68(2015 Aug)

Detailed structural insights into the p97-Npl4-Ufd1 interface.
Isaacson RL, Pye VE, Simpson P, Meyer HH, Zhang X, Freemont PS, Matthews S
The Journal of biological chemistry282(29)21361-9(2007 Jul)

Involvement of the p97-Ufd1-Npl4 complex in the regulated endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors.
Alzayady KJ, Panning MM, Kelley GG, Wojcikiewicz RJ
The Journal of biological chemistry280(41)34530-7(2005 Oct)

A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.
Meyer HH, Shorter JG, Seemann J, Pappin D, Warren G
The EMBO journal19(10)2181-92(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study revealed a regulatory role of the p97-Npl4-Ufd1 complex in regulating a partial degradation of the NF-kappaB subunit p100.
Title: The Transitional Endoplasmic Reticulum ATPase p97 Regulates the Alternative Nuclear Factor NF-?B Signaling via Partial Degradation of the NF-?B Subunit p100.

structural analysis of the p97-Npl4-Ufd1 interface
Title: Detailed structural insights into the p97-Npl4-Ufd1 interface.

the p97-Ufd1-Npl4 complex couples ubiquitinated IP(3) receptors to proteasomal degradation and, thus, plays a key role in IP(3) receptor processing
Title: Involvement of the p97-Ufd1-Npl4 complex in the regulated endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors.

The following Nploc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nploc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16206	NM_001195023.1 Latest version!	Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu46199	XM_006533134.2 Latest version!	Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu16093	NM_199469.2 Latest version!	Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nploc4 ( NM_001195023.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001181952.1 Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OMu16206
Clone ID Related Accession (Same CDS sequence)	NM_001195023.1
Accession Version	NM_001195023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	nuclear protein localization protein 4 homolog isoform A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL669855.20 and AK149912.1. Transcript Variant: This variant (A) represents the longer transcript and encodes the longer isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK149823.1, SRR1660815.118554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGA GCATCATAAT CCGTGTCCAG TCCCCTGATG GCGTGAAGAG GATCACAGCT 
ACGAAGAGAG AGACGGCTGC CACCTTCCTG AAAAAGGTTG CAAAGGAGTT TGGCTTCCAA 
AACAACGGCT TCTCAGTTTA CATCAATAGA AACAAGACTG GAGAGATAAC AGCATCGTCC 
AGCAAATCCC TCCATTTGCT AAAAATCAAG CATGGCGATT TGTTGTTCCT GTTTCCCTCA 
AGCCTTGCTG GACCCTCGTC TGAAATGGAA ACATCGACCT CGGTAGGGTT AAAGGCCTTT 
GGTGCTCCTA ACGTGGTTGA AGATGAGATC GATCAATACC TCAGCAAGCA GGATGGGAAG 
ATTTACCGGA GCCGAGACCC ACAGCTATGC CGCCATGGTC CCTTGGGCAA GTGTGTGCAT 
TGTGTCCCAC TGGAGCCATT TGATGAGGAC TATCTGAACC ACTTGGAGCC TCCTGTGAAG 
CACATGTCCT TCCATGCCTA CATCCGGAAG CTGACTGGAG GGGCTGACAA GGGTAAGTTT 
GTTGCCCTGG AGAACATCAG CTGCAAGATT AAGTCAGGGT GTGAGGGACA CCTCCCATGG 
CCAAATGGTA TCTGCACCAA ATGCCAGCCC AGTGCCATCA CGCTGAACAG ACAGAAATAC 
CGACACGTGG ACAACATCAT GTTTGAGAAT CATACTGTTG CTGACCGCTT CCTTGACTTC 
TGGAGAAAGA CCGGAAACCA GCATTTTGGG TACTTATACG GACGGTACAC AGAGCACAAG 
GACATTCCCC TCGGCATCAG GGCTGAAGTG GCTGCAATTT ACGAGCCTCC TCAGATTGGT 
ACACAGAACA GTCTGGAGCT TCTGGAAGAC CCGAAAGCTG AGGTGGTTGA CGAGATTGCC 
GCCAAACTTG GCCTGAGGAA GGTTGGATGG ATATTCACGG ACCTTGTCTC AGAAGACACC 
CGAAAGGGTA CAGTCCGATA CAGTCGGAAT AAGGACACCT ATTTCCTGAG TTCAGAAGAA 
TGCATCACTG CAGGAGACTT TCAAAACAAG CACCCCAACA TTTGCCGGCT CTCTCCCGAT 
GGTCATTTTG GATCCAAGTT TGTTACTGCA GTGGCCACAG GTGGTCCTGA CAACCAGGTT 
CACTTTGAAG GGTACCAGGT ATCCAATCAG TGCATGGCAC TAGTCCGAGA TGAGTGTCTT 
CTACCTTGTA AGGATGCCCC TGAGCTTGGC TATGCTAAGG AGTCCAGCAG TGAGCAGTAT 
GTTCCTGATG TGTTTTATAA GGACATAGAC AAGTTTGGCA ATGAGATCAC CCAGCTGGCC 
CGTCCCCTGC CTGTGGAGTA TCTCATCATA GATATCACAA CCACCTTCCC CAAGGATCCA 
GTTTACACTT TTTCTATTTC TCAAAATCCG TTTCCTATCG AAAACCGGGA TGTACTGGGT 
GAAACACAGG ACTTCCATAG TTTGGCCACC TACTTGTCCC AGAATACCTC ATCCGTGTTC 
TTGGATACCA TTTCCGACTT CCATCTCCTG CTGTTTCTGG TCACCAATGA AGTCATGCCG 
CTGCAGGACA GTATCAGCCT GCTGCTAGAA GCCGTGAGGA CCAGAAATGA GGAGCTAGCA 
CAGACATGGA AAAAGTCTGA ACAGTGGGCC ACCATTGAGC AGTTATGCAG CACCGTTGGT 
GTGCAGCTCC CAGGCCTCCA CGAGTTTGGT GCAGTCGGGG GCTCTGCACG CGCTGCTACC 
TCTGCCATGT GGGCCTGTCA GCACTGCACC TTCATGAACC AACCTGGCAC AGGCCACTGC 
GAGATGTGCA GCCTCCCCCG GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181952.1
CDS	188..2014
Translation	MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSSKSLHLLKIKHGDLLFLFPSSLAGPSSEMETSTSVGLKAFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNICRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDIDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKKSEQWATIEQLCSTVGVQLPGLHEFGAVGGSARAATSAMWACQHCTFMNQPGTGHCEMCSLPRT

Target ORF information:

RefSeq Version	NM_001195023.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195023.1

ORF Insert Sequence:

ATGGCCGAGA GCATCATAAT CCGTGTCCAG TCCCCTGATG GCGTGAAGAG GATCACAGCT 
ACGAAGAGAG AGACGGCTGC CACCTTCCTG AAAAAGGTTG CAAAGGAGTT TGGCTTCCAA 
AACAACGGCT TCTCAGTTTA CATCAATAGA AACAAGACTG GAGAGATAAC AGCATCGTCC 
AGCAAATCCC TCCATTTGCT AAAAATCAAG CATGGCGATT TGTTGTTCCT GTTTCCCTCA 
AGCCTTGCTG GACCCTCGTC TGAAATGGAA ACATCGACCT CGGTAGGGTT AAAGGCCTTT 
GGTGCTCCTA ACGTGGTTGA AGATGAGATC GATCAATACC TCAGCAAGCA GGATGGGAAG 
ATTTACCGGA GCCGAGACCC ACAGCTATGC CGCCATGGTC CCTTGGGCAA GTGTGTGCAT 
TGTGTCCCAC TGGAGCCATT TGATGAGGAC TATCTGAACC ACTTGGAGCC TCCTGTGAAG 
CACATGTCCT TCCATGCCTA CATCCGGAAG CTGACTGGAG GGGCTGACAA GGGTAAGTTT 
GTTGCCCTGG AGAACATCAG CTGCAAGATT AAGTCAGGGT GTGAGGGACA CCTCCCATGG 
CCAAATGGTA TCTGCACCAA ATGCCAGCCC AGTGCCATCA CGCTGAACAG ACAGAAATAC 
CGACACGTGG ACAACATCAT GTTTGAGAAT CATACTGTTG CTGACCGCTT CCTTGACTTC 
TGGAGAAAGA CCGGAAACCA GCATTTTGGG TACTTATACG GACGGTACAC AGAGCACAAG 
GACATTCCCC TCGGCATCAG GGCTGAAGTG GCTGCAATTT ACGAGCCTCC TCAGATTGGT 
ACACAGAACA GTCTGGAGCT TCTGGAAGAC CCGAAAGCTG AGGTGGTTGA CGAGATTGCC 
GCCAAACTTG GCCTGAGGAA GGTTGGATGG ATATTCACGG ACCTTGTCTC AGAAGACACC 
CGAAAGGGTA CAGTCCGATA CAGTCGGAAT AAGGACACCT ATTTCCTGAG TTCAGAAGAA 
TGCATCACTG CAGGAGACTT TCAAAACAAG CACCCCAACA TTTGCCGGCT CTCTCCCGAT 
GGTCATTTTG GATCCAAGTT TGTTACTGCA GTGGCCACAG GTGGTCCTGA CAACCAGGTT 
CACTTTGAAG GGTACCAGGT ATCCAATCAG TGCATGGCAC TAGTCCGAGA TGAGTGTCTT 
CTACCTTGTA AGGATGCCCC TGAGCTTGGC TATGCTAAGG AGTCCAGCAG TGAGCAGTAT 
GTTCCTGATG TGTTTTATAA GGACATAGAC AAGTTTGGCA ATGAGATCAC CCAGCTGGCC 
CGTCCCCTGC CTGTGGAGTA TCTCATCATA GATATCACAA CCACCTTCCC CAAGGATCCA 
GTTTACACTT TTTCTATTTC TCAAAATCCG TTTCCTATCG AAAACCGGGA TGTACTGGGT 
GAAACACAGG ACTTCCATAG TTTGGCCACC TACTTGTCCC AGAATACCTC ATCCGTGTTC 
TTGGATACCA TTTCCGACTT CCATCTCCTG CTGTTTCTGG TCACCAATGA AGTCATGCCG 
CTGCAGGACA GTATCAGCCT GCTGCTAGAA GCCGTGAGGA CCAGAAATGA GGAGCTAGCA 
CAGACATGGA AAAAGTCTGA ACAGTGGGCC ACCATTGAGC AGTTATGCAG CACCGTTGGT 
GTGCAGCTCC CAGGCCTCCA CGAGTTTGGT GCAGTCGGGG GCTCTGCACG CGCTGCTACC 
TCTGCCATGT GGGCCTGTCA GCACTGCACC TTCATGAACC AACCTGGCAC AGGCCACTGC 
GAGATGTGCA GCCTCCCCCG GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nploc4 ( XM_006533134.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533197.1 Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46199
Clone ID Related Accession (Same CDS sequence)	XM_006533134.2
Accession Version	XM_006533134.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear protein localization protein 4 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533134.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAT CGACCTCGGT AGGGTTAAAG GCCTTTGGTG CTCCTAACGT GGTTGAAGAT 
GAGATCGATC AATACCTCAG CAAGCAGGAT GGGAAGATTT ACCGGAGCCG AGACCCACAG 
CTATGCCGCC ATGGTCCCTT GGGCAAGTGT GTGCATTGTG TCCCACTGGA GCCATTTGAT 
GAGGACTATC TGAACCACTT GGAGCCTCCT GTGAAGCACA TGTCCTTCCA TGCCTACATC 
CGGAAGCTGA CTGGAGGGGC TGACAAGGGT AAGTTTGTTG CCCTGGAGAA CATCAGCTGC 
AAGATTAAGT CAGGGTGTGA GGGACACCTC CCATGGCCAA ATGGTATCTG CACCAAATGC 
CAGCCCAGTG CCATCACGCT GAACAGACAG AAATACCGAC ACGTGGACAA CATCATGTTT 
GAGAATCATA CTGTTGCTGA CCGCTTCCTT GACTTCTGGA GAAAGACCGG AAACCAGCAT 
TTTGGGTACT TATACGGACG GTACACAGAG CACAAGGACA TTCCCCTCGG CATCAGGGCT 
GAAGTGGCTG CAATTTACGA GCCTCCTCAG ATTGGTACAC AGAACAGTCT GGAGCTTCTG 
GAAGACCCGA AAGCTGAGGT GGTTGACGAG ATTGCCGCCA AACTTGGCCT GAGGAAGGTT 
GGATGGATAT TCACGGACCT TGTCTCAGAA GACACCCGAA AGGGTACAGT CCGATACAGT 
CGGAATAAGG ACACCTATTT CCTGAGTTCA GAAGAATGCA TCACTGCAGG AGACTTTCAA 
AACAAGCACC CCAACATTTG CCGGCTCTCT CCCGATGGTC ATTTTGGATC CAAGTTTGTT 
ACTGCAGTGG CCACAGGTGG TCCTGACAAC CAGGTTCACT TTGAAGGGTA CCAGGTATCC 
AATCAGTGCA TGGCACTAGT CCGAGATGAG TGTCTTCTAC CTTGTAAGGA TGCCCCTGAG 
CTTGGCTATG CTAAGGAGTC CAGCAGTGAG CAGTATGTTC CTGATGTGTT TTATAAGGAC 
ATAGACAAGT TTGGCAATGA GATCACCCAG CTGGCCCGTC CCCTGCCTGT GGAGTATCTC 
ATCATAGATA TCACAACCAC CTTCCCCAAG GATCCAGTTT ACACTTTTTC TATTTCTCAA 
AATCCGTTTC CTATCGAAAA CCGGGATGTA CTGGGTGAAA CACAGGACTT CCATAGTTTG 
GCCACCTACT TGTCCCAGAA TACCTCATCC GTGTTCTTGG ATACCATTTC CGACTTCCAT 
CTCCTGCTGT TTCTGGTCAC CAATGAAGTC ATGCCGCTGC AGGACAGTAT CAGCCTGCTG 
CTAGAAGCCG TGAGGACCAG AAATGAGGAG CTAGCACAGA CATGGAAAAA GTCTGAACAG 
TGGGCCACCA TTGAGCAGTT ATGCAGCACC GTTGGTGTGC AGCTCCCAGG CCTCCACGAG 
TTTGGTGCAG TCGGGGGCTC TGCACGCGCT GCTACCTCTG CCATGTGGGC CTGTCAGCAC 
TGCACCTTCA TGAACCAACC TGGCACAGGC CACTGCGAGA TGTGCAGCCT CCCCCGGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533197.1
CDS	153..1715
Translation	METSTSVGLKAFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNICRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDIDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKKSEQWATIEQLCSTVGVQLPGLHEFGAVGGSARAATSAMWACQHCTFMNQPGTGHCEMCSLPRT

Target ORF information:

RefSeq Version	XM_006533134.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533134.2

ORF Insert Sequence:

ATGGAAACAT CGACCTCGGT AGGGTTAAAG GCCTTTGGTG CTCCTAACGT GGTTGAAGAT 
GAGATCGATC AATACCTCAG CAAGCAGGAT GGGAAGATTT ACCGGAGCCG AGACCCACAG 
CTATGCCGCC ATGGTCCCTT GGGCAAGTGT GTGCATTGTG TCCCACTGGA GCCATTTGAT 
GAGGACTATC TGAACCACTT GGAGCCTCCT GTGAAGCACA TGTCCTTCCA TGCCTACATC 
CGGAAGCTGA CTGGAGGGGC TGACAAGGGT AAGTTTGTTG CCCTGGAGAA CATCAGCTGC 
AAGATTAAGT CAGGGTGTGA GGGACACCTC CCATGGCCAA ATGGTATCTG CACCAAATGC 
CAGCCCAGTG CCATCACGCT GAACAGACAG AAATACCGAC ACGTGGACAA CATCATGTTT 
GAGAATCATA CTGTTGCTGA CCGCTTCCTT GACTTCTGGA GAAAGACCGG AAACCAGCAT 
TTTGGGTACT TATACGGACG GTACACAGAG CACAAGGACA TTCCCCTCGG CATCAGGGCT 
GAAGTGGCTG CAATTTACGA GCCTCCTCAG ATTGGTACAC AGAACAGTCT GGAGCTTCTG 
GAAGACCCGA AAGCTGAGGT GGTTGACGAG ATTGCCGCCA AACTTGGCCT GAGGAAGGTT 
GGATGGATAT TCACGGACCT TGTCTCAGAA GACACCCGAA AGGGTACAGT CCGATACAGT 
CGGAATAAGG ACACCTATTT CCTGAGTTCA GAAGAATGCA TCACTGCAGG AGACTTTCAA 
AACAAGCACC CCAACATTTG CCGGCTCTCT CCCGATGGTC ATTTTGGATC CAAGTTTGTT 
ACTGCAGTGG CCACAGGTGG TCCTGACAAC CAGGTTCACT TTGAAGGGTA CCAGGTATCC 
AATCAGTGCA TGGCACTAGT CCGAGATGAG TGTCTTCTAC CTTGTAAGGA TGCCCCTGAG 
CTTGGCTATG CTAAGGAGTC CAGCAGTGAG CAGTATGTTC CTGATGTGTT TTATAAGGAC 
ATAGACAAGT TTGGCAATGA GATCACCCAG CTGGCCCGTC CCCTGCCTGT GGAGTATCTC 
ATCATAGATA TCACAACCAC CTTCCCCAAG GATCCAGTTT ACACTTTTTC TATTTCTCAA 
AATCCGTTTC CTATCGAAAA CCGGGATGTA CTGGGTGAAA CACAGGACTT CCATAGTTTG 
GCCACCTACT TGTCCCAGAA TACCTCATCC GTGTTCTTGG ATACCATTTC CGACTTCCAT 
CTCCTGCTGT TTCTGGTCAC CAATGAAGTC ATGCCGCTGC AGGACAGTAT CAGCCTGCTG 
CTAGAAGCCG TGAGGACCAG AAATGAGGAG CTAGCACAGA CATGGAAAAA GTCTGAACAG 
TGGGCCACCA TTGAGCAGTT ATGCAGCACC GTTGGTGTGC AGCTCCCAGG CCTCCACGAG 
TTTGGTGCAG TCGGGGGCTC TGCACGCGCT GCTACCTCTG CCATGTGGGC CTGTCAGCAC 
TGCACCTTCA TGAACCAACC TGGCACAGGC CACTGCGAGA TGTGCAGCCT CCCCCGGACC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nploc4 ( NM_199469.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_955763.1 Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant B, mRNA.

Price & Availability↑

CloneID	OMu16093
Clone ID Related Accession (Same CDS sequence)	NM_199469.2
Accession Version	NM_199469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	nuclear protein localization protein 4 homolog isoform B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL669855.20 and BC065156.1. On Aug 18, 2010 this sequence version replaced NM_199469.1. Transcript Variant: This variant (B) lacks an in-frame exon in the coding region, compared to variant A. This results in a shorter protein (isoform B), compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC065156.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGA GCATCATAAT CCGTGTCCAG TCCCCTGATG GCGTGAAGAG GATCACAGCT 
ACGAAGAGAG AGACGGCTGC CACCTTCCTG AAAAAGGTTG CAAAGGAGTT TGGCTTCCAA 
AACAACGGCT TCTCAGTTTA CATCAATAGA AACAAGACTG GAGAGATAAC AGCATCGTCC 
AGCAAATCCC TCCATTTGCT AAAAATCAAG CATGGCGATT TGTTGTTCCT GTTTCCCTCA 
AGCCTTGCTG GACCCTCGTC TGAAATGGAA ACATCGACCT CGGTAGGGTT AAAGGCCTTT 
GGTGCTCCTA ACGTGGTTGA AGATGAGATC GATCAATACC TCAGCAAGCA GGATGGGAAG 
ATTTACCGGA GCCGAGACCC ACAGCTATGC CGCCATGGTC CCTTGGGCAA GTGTGTGCAT 
TGTGTCCCAC TGGAGCCATT TGATGAGGAC TATCTGAACC ACTTGGAGCC TCCTGTGAAG 
CACATGTCCT TCCATGCCTA CATCCGGAAG CTGACTGGAG GGGCTGACAA GGGTAAGTTT 
GTTGCCCTGG AGAACATCAG CTGCAAGATT AAGTCAGGGT GTGAGGGACA CCTCCCATGG 
CCAAATGGTA TCTGCACCAA ATGCCAGCCC AGTGCCATCA CGCTGAACAG ACAGAAATAC 
CGACACGTGG ACAACATCAT GTTTGAGAAT CATACTGTTG CTGACCGCTT CCTTGACTTC 
TGGAGAAAGA CCGGAAACCA GCATTTTGGG TACTTATACG GACGGTACAC AGAGCACAAG 
GACATTCCCC TCGGCATCAG GGCTGAAGTG GCTGCAATTT ACGAGCCTCC TCAGATTGGT 
ACACAGAACA GTCTGGAGCT TCTGGAAGAC CCGAAAGCTG AGGTGGTTGA CGAGATTGCC 
GCCAAACTTG GCCTGAGGAA GGTTGGATGG ATATTCACGG ACCTTGTCTC AGAAGACACC 
CGAAAGGGTA CAGTCCGATA CAGTCGGAAT AAGGACACCT ATTTCCTGAG TTCAGAAGAA 
TGCATCACTG CAGGAGACTT TCAAAACAAG CACCCCAACA TTTGCCGGCT CTCTCCCGAT 
GGTCATTTTG GATCCAAGTT TGTTACTGCA GTGGCCACAG GTGGTCCTGA CAACCAGGTT 
CACTTTGAAG GGTACCAGGT ATCCAATCAG TGCATGGCAC TAGTCCGAGA TGAGTGTCTT 
CTACCTTGTA AGGATGCCCC TGAGCTTGGC TATGCTAAGG AGTCCAGCAG TGAGCAGTAT 
GTTCCTGATG TGTTTTATAA GGACATAGAC AAGTTTGGCA ATGAGATCAC CCAGCTGGCC 
CGTCCCCTGC CTGTGGAGTA TCTCATCATA GATGACTTCC ATAGTTTGGC CACCTACTTG 
TCCCAGAATA CCTCATCCGT GTTCTTGGAT ACCATTTCCG ACTTCCATCT CCTGCTGTTT 
CTGGTCACCA ATGAAGTCAT GCCGCTGCAG GACAGTATCA GCCTGCTGCT AGAAGCCGTG 
AGGACCAGAA ATGAGGAGCT AGCACAGACA TGGAAAAAGT CTGAACAGTG GGCCACCATT 
GAGCAGTTAT GCAGCACCGT TGGTGTGCAG CTCCCAGGCC TCCACGAGTT TGGTGCAGTC 
GGGGGCTCTG CACGCGCTGC TACCTCTGCC ATGTGGGCCT GTCAGCACTG CACCTTCATG 
AACCAACCTG GCACAGGCCA CTGCGAGATG TGCAGCCTCC CCCGGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955763.1
CDS	188..1918
Translation	MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSSKSLHLLKIKHGDLLFLFPSSLAGPSSEMETSTSVGLKAFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNICRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDIDKFGNEITQLARPLPVEYLIIDDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKKSEQWATIEQLCSTVGVQLPGLHEFGAVGGSARAATSAMWACQHCTFMNQPGTGHCEMCSLPRT

Target ORF information:

RefSeq Version	NM_199469.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199469.2

ORF Insert Sequence:

ATGGCCGAGA GCATCATAAT CCGTGTCCAG TCCCCTGATG GCGTGAAGAG GATCACAGCT 
ACGAAGAGAG AGACGGCTGC CACCTTCCTG AAAAAGGTTG CAAAGGAGTT TGGCTTCCAA 
AACAACGGCT TCTCAGTTTA CATCAATAGA AACAAGACTG GAGAGATAAC AGCATCGTCC 
AGCAAATCCC TCCATTTGCT AAAAATCAAG CATGGCGATT TGTTGTTCCT GTTTCCCTCA 
AGCCTTGCTG GACCCTCGTC TGAAATGGAA ACATCGACCT CGGTAGGGTT AAAGGCCTTT 
GGTGCTCCTA ACGTGGTTGA AGATGAGATC GATCAATACC TCAGCAAGCA GGATGGGAAG 
ATTTACCGGA GCCGAGACCC ACAGCTATGC CGCCATGGTC CCTTGGGCAA GTGTGTGCAT 
TGTGTCCCAC TGGAGCCATT TGATGAGGAC TATCTGAACC ACTTGGAGCC TCCTGTGAAG 
CACATGTCCT TCCATGCCTA CATCCGGAAG CTGACTGGAG GGGCTGACAA GGGTAAGTTT 
GTTGCCCTGG AGAACATCAG CTGCAAGATT AAGTCAGGGT GTGAGGGACA CCTCCCATGG 
CCAAATGGTA TCTGCACCAA ATGCCAGCCC AGTGCCATCA CGCTGAACAG ACAGAAATAC 
CGACACGTGG ACAACATCAT GTTTGAGAAT CATACTGTTG CTGACCGCTT CCTTGACTTC 
TGGAGAAAGA CCGGAAACCA GCATTTTGGG TACTTATACG GACGGTACAC AGAGCACAAG 
GACATTCCCC TCGGCATCAG GGCTGAAGTG GCTGCAATTT ACGAGCCTCC TCAGATTGGT 
ACACAGAACA GTCTGGAGCT TCTGGAAGAC CCGAAAGCTG AGGTGGTTGA CGAGATTGCC 
GCCAAACTTG GCCTGAGGAA GGTTGGATGG ATATTCACGG ACCTTGTCTC AGAAGACACC 
CGAAAGGGTA CAGTCCGATA CAGTCGGAAT AAGGACACCT ATTTCCTGAG TTCAGAAGAA 
TGCATCACTG CAGGAGACTT TCAAAACAAG CACCCCAACA TTTGCCGGCT CTCTCCCGAT 
GGTCATTTTG GATCCAAGTT TGTTACTGCA GTGGCCACAG GTGGTCCTGA CAACCAGGTT 
CACTTTGAAG GGTACCAGGT ATCCAATCAG TGCATGGCAC TAGTCCGAGA TGAGTGTCTT 
CTACCTTGTA AGGATGCCCC TGAGCTTGGC TATGCTAAGG AGTCCAGCAG TGAGCAGTAT 
GTTCCTGATG TGTTTTATAA GGACATAGAC AAGTTTGGCA ATGAGATCAC CCAGCTGGCC 
CGTCCCCTGC CTGTGGAGTA TCTCATCATA GATGACTTCC ATAGTTTGGC CACCTACTTG 
TCCCAGAATA CCTCATCCGT GTTCTTGGAT ACCATTTCCG ACTTCCATCT CCTGCTGTTT 
CTGGTCACCA ATGAAGTCAT GCCGCTGCAG GACAGTATCA GCCTGCTGCT AGAAGCCGTG 
AGGACCAGAA ATGAGGAGCT AGCACAGACA TGGAAAAAGT CTGAACAGTG GGCCACCATT 
GAGCAGTTAT GCAGCACCGT TGGTGTGCAG CTCCCAGGCC TCCACGAGTT TGGTGCAGTC 
GGGGGCTCTG CACGCGCTGC TACCTCTGCC ATGTGGGCCT GTCAGCACTG CACCTTCATG 
AACCAACCTG GCACAGGCCA CTGCGAGATG TGCAGCCTCC CCCGGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential Involvement of the Npl4 Zinc Finger Domains of SHARPIN and HOIL-1L in Linear Ubiquitin Chain Assembly Complex-Mediated Cell Death Protection.
Molecular and cellular biology36(10)1569-83(2016 05)
Shimizu S,Fujita H,Sasaki Y,Tsuruyama T,Fukuda K,Iwai K

The Transitional Endoplasmic Reticulum ATPase p97 Regulates the Alternative Nuclear Factor NF-?B Signaling via Partial Degradation of the NF-?B Subunit p100.
The Journal of biological chemistry290(32)19558-68(2015 Aug)
Zhang Z,Wang Y,Li C,Shi Z,Hao Q,Wang W,Song X,Zhao Y,Jiao S,Zhou Z

Detailed structural insights into the p97-Npl4-Ufd1 interface.
The Journal of biological chemistry282(29)21361-9(2007 Jul)
Isaacson RL,Pye VE,Simpson P,Meyer HH,Zhang X,Freemont PS,Matthews S

Involvement of the p97-Ufd1-Npl4 complex in the regulated endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors.
The Journal of biological chemistry280(41)34530-7(2005 Oct)
Alzayady KJ,Panning MM,Kelley GG,Wojcikiewicz RJ

A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.
The EMBO journal19(10)2181-92(2000 May)
Meyer HH,Shorter JG,Seemann J,Pappin D,Warren G