Ube2o cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2o
Entrez Gene ID	217342
Full Name	ubiquitin-conjugating enzyme E2O
Synonyms	9630022H21,B230113M03Rik,E2-230K,mKIAA1734
General protein information	Preferred Names ubiquitin-conjugating enzyme E2O Names (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O E2/E3 hybrid ubiquitin-protein ligase UBE2O ubiquitin carrier protein O ubiquitin-conjugating enzyme E2 of 230 kDa ubiquitin-conjugating enzyme E2-230K ubiquitin-protein ligase O ubiquitinating enzyme E2-230 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533106.2	XP_006533169.1	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform X1
XM_006533106.1	XP_006533169.1	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform X1
XM_011248960.1	XP_011247262.1	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform X2
NM_173755.3	NP_776116.2	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2O	XP_001139963.2
Bos taurus (cattle)	UBE2O	XP_002696213.1
Gallus gallus (chicken)	UBE2O	XP_415619.4
Homo sapiens (human)	UBE2O	NP_071349.3
Rattus norvegicus (Norway rat)	Ube2o	XP_221132.5
	UBE2O	XP_001099166.2
Canis lupus familiaris (dog)	UBE2O	XP_540449.3
Mus musculus (house mouse)	Ube2o	NP_776116.2
Xenopus tropicalis (tropical clawed frog)	ube2o	XP_002936000.2

Gene Ontology
Bibliography

Related articles in PubMed

UBE2O remodels the proteome during terminal erythroid differentiation.
Nguyen AT, Prado MA, Schmidt PJ, Sendamarai AK, Wilson-Grady JT, Min M, Campagna DR, Tian G, Shi Y, Dederer V, Kawan M, Kuehnle N, Paulo JA, Yao Y, Weiss MJ, Justice MJ, Gygi SP, Fleming MD, Finley D
Science (New York, N.Y.)357(6350)(2017 08)

A UBE2O-AMPK?2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.
Vila IK, Yao Y, Kim G, Xia W, Kim H, Kim SJ, Park MK, Hwang JP, Gonz?lez-Billalabeitia E, Hung MC, Song SJ, Song MS
Cancer cell31(2)208-224(2017 Feb)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UBE2O recognized ribosomal proteins and other substrates directly, targeting them to proteasomes for degradation.
Title: UBE2O remodels the proteome during terminal erythroid differentiation.

monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis
Title: Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6.

The following Ube2o gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2o cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73017	XM_011248960.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu46179	XM_006533106.2 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu21930	NM_173755.3 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$762.30 $1089.00
View Old Accession Versions >>
OMu46179	XM_006533106.1	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2o ( XM_011248960.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247262.1 Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu73017
Clone ID Related Accession (Same CDS sequence)	XM_011248960.1
Accession Version	XM_011248960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT GGAATGCAAC ATCCGCTCCA ACGACCTTTT CCCTGTGCAC CACCTGGACA 
ACAATGAGTT CTGCCCTGGG GACTTCGTGG TGGACAAGCG AGAGCTGCCC AGACCCTGCT 
GTCTATGGTG TGGTGCAGTC TGGGGATCAT GTTGGCCGTA CCTGCATGGT GAAGTGGTTC 
AAGCTGCGAC CAAGTGGGGA TGACGTGGAG CTCATTGGAG AAGAGGAGGA CGTGAGCGTC 
TATGACATTG CTGACCACCC TGACTTTCGC TTCCGCACAA CTGACATTGT CATCCGCATT 
GGTAACACGG AGGATGGGGC TCTACCCAAG GAGGATGAGC CCTCAGTGGG CCAGGTGGCC 
CGAGTGGATG TCAGCAGCAA AGTGGAAGTG GTGTGGGCTG ACAACTCGAA AACCATCATC 
CTGCCACAGC ACTTATACAA CATCGAGTCT GAGATTGAGG AGTCTGACTA CGACTCCGTG 
GAAGGCAGCT CCAGTGGGGC CTCCTCGGAT GAGTGGGAGG ACGACAGCGA CAGCTGGGAG 
ACCGACAACG GGCTCGTGGA CGATGAGCAC CCCAAGATAG AGGAGCTGGC CGCCATCTTG 
CCTGCCGAGC AGCCCACTGC CCCCGAGGAG GACAAGGGCG TGGTCATCAG TGAGGAGGCG 
GCCACGGCTG CCATCCAGGG CGCCGTGGCC ATGGCTGCCC CCGTGGCAGG GCTGATGGAG 
AAAGCTGGGA AGGACGGGCC TCCCAAGAGT TTCCGGGAGC TGAAGGAGGC CATCAAGATC 
CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTCA CAGGCTCGCC CACCTCGCCC 
ACCGTGGAGC CTGAGAAGCC AACCCGAGAG AAGAAGTTTT TGGACGATAT CAAGAAGCTA 
CAGGAGAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG CTGAGGAGGA GAAGATGGAG 
GCAGTACCCG ACACAGAGCG CAAGGAGGAG AAGCCTGAGG TGCAGTCCCC AGTGAAAGCC 
GAGTGGCCCA GTGAGACACC TGTGCTCTGT CAGCAGTGCG GTGGCAGGCC TGGCGTCACC 
TTCACCAGCG CCAAGGGCGA GGTCTTCTCT GTGCTGGAGT TTGCACCCTC AAACCACTCT 
TTCAAGAAGA TTGAGTTCCA GCCCCCGGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG 
GAGATGGCTC TGCTGGCCAC CTCGCTGCCC GATGGCATCA TGGTCAAGAC TTTTGAGGAC 
AGAATGGACC TCTTCTCTGC ACTGATCAAG GGCCCCACTC GGACTCCCTA TGAAGACGGC 
CTCTACCTAT TTGACATCCA GCTTCCCAAC ATCTACCCTG CGGTGCCCCC CCACTTTTGC 
TACCTCTCCC AGTGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAAGTGTGT 
GTCAGCCTCC TAGGTACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCTAGC 
CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TGAATGAACC ATACTACAAT 
GAAGCTGGCT TTGACAGTGA CCGGGGCCTG CAGGAAGGCT ATGAGAACAG TCGCTGCTAC 
AACGAGATGG CATTAATCCG TGTGGTGCAG TCCATGACCC AGTTGGTGCG ACGACCTCCT 
GAGGTCTTCG AACAGGAGAT TCGGCAGCAC TTCAGTGTGG GTGGCTGGCG GCTGGTGAAC 
CGAATTGAGT CCTGGCTGGA AACCCATGCC ATGCAGGAGA GGGCCCAGGT GATGCCCAAT 
GGGGCCCTTA AAGACAGCAG CTCTCTGGAG CCCATGGCTG CAGCTGAACT CTCAGACTCC 
GGCCGTGAAG AGCCTGAAGA TGTTGGGATG GCCCCTGGGG AGGCGTCCCA GGGCTCAGAC 
TCAGAGGGTG GAGCCCAGGG CCCTGCCTCA GCCAGCAGAG ACCACACAGA GCAGACTGAG 
ACAGCCCCTG ATGCATCCGC ACCTCCCAGC GTTAGACCAA AGAGGAGGAG GAAGAGCTAC 
CGCAGCTTCC TGCCTGAGAA GAGCGGTTAC CCTGACATCG GCTTCCCCCT CTTCCCACTC 
TCCAAGGGTT TCATCAAGAG CATCCGGGGG GTCCTGACAC AGTTCCGTGC TGCCCTGCTG 
GAGGCGGGCA TGCCCGAAAG CACAGAGGAC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247262.1
CDS	51..2246
Translation	MALWNATSAPTTFSLCTTWTTMSSALGTSWWTSESCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPGEASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTEDK

Target ORF information:

RefSeq Version	XM_011248960.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248960.1

ORF Insert Sequence:

ATGGCTCTGT GGAATGCAAC ATCCGCTCCA ACGACCTTTT CCCTGTGCAC CACCTGGACA 
ACAATGAGTT CTGCCCTGGG GACTTCGTGG TGGACAAGCG AGAGCTGCCC AGACCCTGCT 
GTCTATGGTG TGGTGCAGTC TGGGGATCAT GTTGGCCGTA CCTGCATGGT GAAGTGGTTC 
AAGCTGCGAC CAAGTGGGGA TGACGTGGAG CTCATTGGAG AAGAGGAGGA CGTGAGCGTC 
TATGACATTG CTGACCACCC TGACTTTCGC TTCCGCACAA CTGACATTGT CATCCGCATT 
GGTAACACGG AGGATGGGGC TCTACCCAAG GAGGATGAGC CCTCAGTGGG CCAGGTGGCC 
CGAGTGGATG TCAGCAGCAA AGTGGAAGTG GTGTGGGCTG ACAACTCGAA AACCATCATC 
CTGCCACAGC ACTTATACAA CATCGAGTCT GAGATTGAGG AGTCTGACTA CGACTCCGTG 
GAAGGCAGCT CCAGTGGGGC CTCCTCGGAT GAGTGGGAGG ACGACAGCGA CAGCTGGGAG 
ACCGACAACG GGCTCGTGGA CGATGAGCAC CCCAAGATAG AGGAGCTGGC CGCCATCTTG 
CCTGCCGAGC AGCCCACTGC CCCCGAGGAG GACAAGGGCG TGGTCATCAG TGAGGAGGCG 
GCCACGGCTG CCATCCAGGG CGCCGTGGCC ATGGCTGCCC CCGTGGCAGG GCTGATGGAG 
AAAGCTGGGA AGGACGGGCC TCCCAAGAGT TTCCGGGAGC TGAAGGAGGC CATCAAGATC 
CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTCA CAGGCTCGCC CACCTCGCCC 
ACCGTGGAGC CTGAGAAGCC AACCCGAGAG AAGAAGTTTT TGGACGATAT CAAGAAGCTA 
CAGGAGAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG CTGAGGAGGA GAAGATGGAG 
GCAGTACCCG ACACAGAGCG CAAGGAGGAG AAGCCTGAGG TGCAGTCCCC AGTGAAAGCC 
GAGTGGCCCA GTGAGACACC TGTGCTCTGT CAGCAGTGCG GTGGCAGGCC TGGCGTCACC 
TTCACCAGCG CCAAGGGCGA GGTCTTCTCT GTGCTGGAGT TTGCACCCTC AAACCACTCT 
TTCAAGAAGA TTGAGTTCCA GCCCCCGGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG 
GAGATGGCTC TGCTGGCCAC CTCGCTGCCC GATGGCATCA TGGTCAAGAC TTTTGAGGAC 
AGAATGGACC TCTTCTCTGC ACTGATCAAG GGCCCCACTC GGACTCCCTA TGAAGACGGC 
CTCTACCTAT TTGACATCCA GCTTCCCAAC ATCTACCCTG CGGTGCCCCC CCACTTTTGC 
TACCTCTCCC AGTGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAAGTGTGT 
GTCAGCCTCC TAGGTACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCTAGC 
CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TGAATGAACC ATACTACAAT 
GAAGCTGGCT TTGACAGTGA CCGGGGCCTG CAGGAAGGCT ATGAGAACAG TCGCTGCTAC 
AACGAGATGG CATTAATCCG TGTGGTGCAG TCCATGACCC AGTTGGTGCG ACGACCTCCT 
GAGGTCTTCG AACAGGAGAT TCGGCAGCAC TTCAGTGTGG GTGGCTGGCG GCTGGTGAAC 
CGAATTGAGT CCTGGCTGGA AACCCATGCC ATGCAGGAGA GGGCCCAGGT GATGCCCAAT 
GGGGCCCTTA AAGACAGCAG CTCTCTGGAG CCCATGGCTG CAGCTGAACT CTCAGACTCC 
GGCCGTGAAG AGCCTGAAGA TGTTGGGATG GCCCCTGGGG AGGCGTCCCA GGGCTCAGAC 
TCAGAGGGTG GAGCCCAGGG CCCTGCCTCA GCCAGCAGAG ACCACACAGA GCAGACTGAG 
ACAGCCCCTG ATGCATCCGC ACCTCCCAGC GTTAGACCAA AGAGGAGGAG GAAGAGCTAC 
CGCAGCTTCC TGCCTGAGAA GAGCGGTTAC CCTGACATCG GCTTCCCCCT CTTCCCACTC 
TCCAAGGGTT TCATCAAGAG CATCCGGGGG GTCCTGACAC AGTTCCGTGC TGCCCTGCTG 
GAGGCGGGCA TGCCCGAAAG CACAGAGGAC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2o ( XM_006533106.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533169.1 Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46179
Clone ID Related Accession (Same CDS sequence)	XM_006533106.2 , XM_006533106.1
Accession Version	XM_006533106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGCC CGCACCCGCC CAGGCCCAGG CCGCCGCCGC CCCGACCCCG 
GCCGCAGCCC CCGCCGCCGC CGCCCCGCCG CCCGCGCCCG CTACGGACTC GGCCTCAGGG 
CCGTCCTCCG ACTCCGGCCC CGAAGCAGGC TCACAGCGCC TGCTGTTCTC CCACGACCTG 
GTGTCCGGCC GTTACCGCGG CTCGGTGCAC TTCGGGCTGG TACGCCTCAT CCACGGCGAG 
GACTCGGACT CGGAGGGCGA CGATGACGGC CGCGGCAGCT CGGGCTGCTC CGAGGCCGGG 
GGCGCGGGCC ACGAGGAGGG CCGGGCCAGC CCTCTGCGGC GCGGCTACGT GCGCGTCCAG 
TGGTACCCGG AGGGCGTCAA GCAGCATGTG AAGGAGACCA AGCTGAAACT GGAGGACCGC 
TCTGTGGTGC CCCGGGATGT GGTTCGACAC ATGCGCTCAA CTGACAGCCA GTGTGGCACG 
GTGATAGATG TCAACATTGA CTGTGCCGTC AAGCTCATCG GCACCAACTG CATCATCTAC 
CCTGTCAACA GCAAGGACCT CCAGCACATC TGGCCCTTCA TGTATGGAGA CTATATCGCC 
TATGACTGCT GGCTGGGGAA GGTCTATGAC TTGAAGAACC AGATCATCCT CAAGTTGTCT 
AATGGTGCCA GGTGCTCCAT GAACACAGAA GATGGTGCCA AACTCTACGA TGTCTGCCCT 
CACGTCAGCG ACTCGGGTCT CTTCTTTGAT GACTCTTATG GCTTCTACCC AGGCCAAGTC 
CTTATTGGCC CAGCCAAGAT CTTCTCCAGT GTCCAGTGGC TCTCTGGGGT CAAGCCTGTA 
CTCAGCACCA AGAGCAAGTT CCGTGTGGTG GTGGAGGAGG TGCAGGTTGT AGAACTGAAA 
GTCACGTGGA TTACCAAGAG CTTCTGCCCC GGCGGCACGG ACAGCGTTAG CCCCCCACCT 
TCCATCATCA CTCAGGAAAA CCTAGGCAGG GTGAAGCGTC TCGGATGCTT TGACCATGCT 
CAGCGGCAGC TTGGGGAACG CTGTCTGTAT GTCTTCCCAG CCAAGGTGAA GCGTCTGTTG 
AAGAAGCAAG TTGTGAGAAT CATGTCCTGC ACCCCAGATA CCCAGTGTCC TAGGGACCAT 
TCCATGGAAG ACCCAGACAA GAAAGGGGAA GCCAGAGCCG GGAGTGAAAT AGGGTCTGCT 
AGCCCGGAGG AGCAACCTGA TGGATCAGCC AGCCCCGTGG AGATGCAGGA TGAGGGGTCG 
GAGGAGCTTC AGGAGACATG CGAGCCACTG CCCCCCTTCC TCTTGAAGGA GGGTGGAGAT 
GACGGGCTGC ACTCGGCAGA GCAGGACGCA GATGATGAGG CGGCTGACGA TACAGATGAC 
ACCAGCTCTG TGACCTCCTC CGCCAGCTCT ACTACCTCCT CCCAGAGTGG CAGTGGCACT 
GGTCGTAAAA AGAGCATTCC CTTGTCCATC AAAAACTTGA AGCGAAAACA CAAGAGGAAG 
AAGAATAAGG TCACCCGTGA CTTCAAGCCT GGAGACAGGG TGGCAGTGGA GGTTGTGACC 
ACGATGACCT CAGCTGATGT GATGTGGCAA GATGGCTCTG TGGAATGCAA CATCCGCTCC 
AACGACCTTT TCCCTGTGCA CCACCTGGAC AACAATGAGT TCTGCCCTGG GGACTTCGTG 
GTGGACAAGC GAGTCCAGAG CTGCCCAGAC CCTGCTGTCT ATGGTGTGGT GCAGTCTGGG 
GATCATGTTG GCCGTACCTG CATGGTGAAG TGGTTCAAGC TGCGACCAAG TGGGGATGAC 
GTGGAGCTCA TTGGAGAAGA GGAGGACGTG AGCGTCTATG ACATTGCTGA CCACCCTGAC 
TTTCGCTTCC GCACAACTGA CATTGTCATC CGCATTGGTA ACACGGAGGA TGGGGCTCTA 
CCCAAGGAGG ATGAGCCCTC AGTGGGCCAG GTGGCCCGAG TGGATGTCAG CAGCAAAGTG 
GAAGTGGTGT GGGCTGACAA CTCGAAAACC ATCATCCTGC CACAGCACTT ATACAACATC 
GAGTCTGAGA TTGAGGAGTC TGACTACGAC TCCGTGGAAG GCAGCTCCAG TGGGGCCTCC 
TCGGATGAGT GGGAGGACGA CAGCGACAGC TGGGAGACCG ACAACGGGCT CGTGGACGAT 
GAGCACCCCA AGATAGAGGA GCTGGCCGCC ATCTTGCCTG CCGAGCAGCC CACTGCCCCC 
GAGGAGGACA AGGGCGTGGT CATCAGTGAG GAGGCGGCCA CGGCTGCCAT CCAGGGCGCC 
GTGGCCATGG CTGCCCCCGT GGCAGGGCTG ATGGAGAAAG CTGGGAAGGA CGGGCCTCCC 
AAGAGTTTCC GGGAGCTGAA GGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT 
GTGGAGCAGC TGCTCACAGG CTCGCCCACC TCGCCCACCG TGGAGCCTGA GAAGCCAACC 
CGAGAGAAGA AGTTTTTGGA CGATATCAAG AAGCTACAGG AGAACCTCAA GAAGACCCTG 
GACAATGTGG CCATTGCTGA GGAGGAGAAG ATGGAGGCAG TACCCGACAC AGAGCGCAAG 
GAGGAGAAGC CTGAGGTGCA GTCCCCAGTG AAAGCCGAGT GGCCCAGTGA GACACCTGTG 
CTCTGTCAGC AGTGCGGTGG CAGGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC 
TTCTCTGTGC TGGAGTTTGC ACCCTCAAAC CACTCTTTCA AGAAGATTGA GTTCCAGCCC 
CCGGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCTCTGCT GGCCACCTCG 
CTGCCCGATG GCATCATGGT CAAGACTTTT GAGGACAGAA TGGACCTCTT CTCTGCACTG 
ATCAAGGGCC CCACTCGGAC TCCCTATGAA GACGGCCTCT ACCTATTTGA CATCCAGCTT 
CCCAACATCT ACCCTGCGGT GCCCCCCCAC TTTTGCTACC TCTCCCAGTG CAGTGGCCGC 
CTGAACCCCA ACCTGTATGA CAATGGGAAA GTGTGTGTCA GCCTCCTAGG TACCTGGATT 
GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCTAGCCTTC TCCAGGTGCT CATCTCCATC 
CAAGGTCTGA TCCTGGTGAA TGAACCATAC TACAATGAAG CTGGCTTTGA CAGTGACCGG 
GGCCTGCAGG AAGGCTATGA GAACAGTCGC TGCTACAACG AGATGGCATT AATCCGTGTG 
GTGCAGTCCA TGACCCAGTT GGTGCGACGA CCTCCTGAGG TCTTCGAACA GGAGATTCGG 
CAGCACTTCA GTGTGGGTGG CTGGCGGCTG GTGAACCGAA TTGAGTCCTG GCTGGAAACC 
CATGCCATGC AGGAGAGGGC CCAGGTGATG CCCAATGGGG CCCTTAAAGA CAGCAGCTCT 
CTGGAGCCCA TGGCTGCAGC TGAACTCTCA GACTCCGGCC GTGAAGAGCC TGAAGATGTT 
GGGATGGCCC CTGGGGAGGC GTCCCAGGGC TCAGACTCAG AGGGTGGAGC CCAGGGCCCT 
GCCTCAGCCA GCAGAGACCA CACAGAGCAG ACTGAGACAG CCCCTGATGC ATCCGCACCT 
CCCAGCGTTA GACCAAAGAG GAGGAGGAAG AGCTACCGCA GCTTCCTGCC TGAGAAGAGC 
GGTTACCCTG ACATCGGCTT CCCCCTCTTC CCACTCTCCA AGGGTTTCAT CAAGAGCATC 
CGGGGGGTCC TGACACAGTT CCGTGCTGCC CTGCTGGAGG CGGGCATGCC CGAAAGCACA 
GAGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533169.1
CDS	91..3882
Translation	MADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPGEASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTEDK

Target ORF information:

RefSeq Version	XM_006533106.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533106.2

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGCC CGCACCCGCC CAGGCCCAGG CCGCCGCCGC CCCGACCCCG 
GCCGCAGCCC CCGCCGCCGC CGCCCCGCCG CCCGCGCCCG CTACGGACTC GGCCTCAGGG 
CCGTCCTCCG ACTCCGGCCC CGAAGCAGGC TCACAGCGCC TGCTGTTCTC CCACGACCTG 
GTGTCCGGCC GTTACCGCGG CTCGGTGCAC TTCGGGCTGG TACGCCTCAT CCACGGCGAG 
GACTCGGACT CGGAGGGCGA CGATGACGGC CGCGGCAGCT CGGGCTGCTC CGAGGCCGGG 
GGCGCGGGCC ACGAGGAGGG CCGGGCCAGC CCTCTGCGGC GCGGCTACGT GCGCGTCCAG 
TGGTACCCGG AGGGCGTCAA GCAGCATGTG AAGGAGACCA AGCTGAAACT GGAGGACCGC 
TCTGTGGTGC CCCGGGATGT GGTTCGACAC ATGCGCTCAA CTGACAGCCA GTGTGGCACG 
GTGATAGATG TCAACATTGA CTGTGCCGTC AAGCTCATCG GCACCAACTG CATCATCTAC 
CCTGTCAACA GCAAGGACCT CCAGCACATC TGGCCCTTCA TGTATGGAGA CTATATCGCC 
TATGACTGCT GGCTGGGGAA GGTCTATGAC TTGAAGAACC AGATCATCCT CAAGTTGTCT 
AATGGTGCCA GGTGCTCCAT GAACACAGAA GATGGTGCCA AACTCTACGA TGTCTGCCCT 
CACGTCAGCG ACTCGGGTCT CTTCTTTGAT GACTCTTATG GCTTCTACCC AGGCCAAGTC 
CTTATTGGCC CAGCCAAGAT CTTCTCCAGT GTCCAGTGGC TCTCTGGGGT CAAGCCTGTA 
CTCAGCACCA AGAGCAAGTT CCGTGTGGTG GTGGAGGAGG TGCAGGTTGT AGAACTGAAA 
GTCACGTGGA TTACCAAGAG CTTCTGCCCC GGCGGCACGG ACAGCGTTAG CCCCCCACCT 
TCCATCATCA CTCAGGAAAA CCTAGGCAGG GTGAAGCGTC TCGGATGCTT TGACCATGCT 
CAGCGGCAGC TTGGGGAACG CTGTCTGTAT GTCTTCCCAG CCAAGGTGAA GCGTCTGTTG 
AAGAAGCAAG TTGTGAGAAT CATGTCCTGC ACCCCAGATA CCCAGTGTCC TAGGGACCAT 
TCCATGGAAG ACCCAGACAA GAAAGGGGAA GCCAGAGCCG GGAGTGAAAT AGGGTCTGCT 
AGCCCGGAGG AGCAACCTGA TGGATCAGCC AGCCCCGTGG AGATGCAGGA TGAGGGGTCG 
GAGGAGCTTC AGGAGACATG CGAGCCACTG CCCCCCTTCC TCTTGAAGGA GGGTGGAGAT 
GACGGGCTGC ACTCGGCAGA GCAGGACGCA GATGATGAGG CGGCTGACGA TACAGATGAC 
ACCAGCTCTG TGACCTCCTC CGCCAGCTCT ACTACCTCCT CCCAGAGTGG CAGTGGCACT 
GGTCGTAAAA AGAGCATTCC CTTGTCCATC AAAAACTTGA AGCGAAAACA CAAGAGGAAG 
AAGAATAAGG TCACCCGTGA CTTCAAGCCT GGAGACAGGG TGGCAGTGGA GGTTGTGACC 
ACGATGACCT CAGCTGATGT GATGTGGCAA GATGGCTCTG TGGAATGCAA CATCCGCTCC 
AACGACCTTT TCCCTGTGCA CCACCTGGAC AACAATGAGT TCTGCCCTGG GGACTTCGTG 
GTGGACAAGC GAGTCCAGAG CTGCCCAGAC CCTGCTGTCT ATGGTGTGGT GCAGTCTGGG 
GATCATGTTG GCCGTACCTG CATGGTGAAG TGGTTCAAGC TGCGACCAAG TGGGGATGAC 
GTGGAGCTCA TTGGAGAAGA GGAGGACGTG AGCGTCTATG ACATTGCTGA CCACCCTGAC 
TTTCGCTTCC GCACAACTGA CATTGTCATC CGCATTGGTA ACACGGAGGA TGGGGCTCTA 
CCCAAGGAGG ATGAGCCCTC AGTGGGCCAG GTGGCCCGAG TGGATGTCAG CAGCAAAGTG 
GAAGTGGTGT GGGCTGACAA CTCGAAAACC ATCATCCTGC CACAGCACTT ATACAACATC 
GAGTCTGAGA TTGAGGAGTC TGACTACGAC TCCGTGGAAG GCAGCTCCAG TGGGGCCTCC 
TCGGATGAGT GGGAGGACGA CAGCGACAGC TGGGAGACCG ACAACGGGCT CGTGGACGAT 
GAGCACCCCA AGATAGAGGA GCTGGCCGCC ATCTTGCCTG CCGAGCAGCC CACTGCCCCC 
GAGGAGGACA AGGGCGTGGT CATCAGTGAG GAGGCGGCCA CGGCTGCCAT CCAGGGCGCC 
GTGGCCATGG CTGCCCCCGT GGCAGGGCTG ATGGAGAAAG CTGGGAAGGA CGGGCCTCCC 
AAGAGTTTCC GGGAGCTGAA GGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT 
GTGGAGCAGC TGCTCACAGG CTCGCCCACC TCGCCCACCG TGGAGCCTGA GAAGCCAACC 
CGAGAGAAGA AGTTTTTGGA CGATATCAAG AAGCTACAGG AGAACCTCAA GAAGACCCTG 
GACAATGTGG CCATTGCTGA GGAGGAGAAG ATGGAGGCAG TACCCGACAC AGAGCGCAAG 
GAGGAGAAGC CTGAGGTGCA GTCCCCAGTG AAAGCCGAGT GGCCCAGTGA GACACCTGTG 
CTCTGTCAGC AGTGCGGTGG CAGGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC 
TTCTCTGTGC TGGAGTTTGC ACCCTCAAAC CACTCTTTCA AGAAGATTGA GTTCCAGCCC 
CCGGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCTCTGCT GGCCACCTCG 
CTGCCCGATG GCATCATGGT CAAGACTTTT GAGGACAGAA TGGACCTCTT CTCTGCACTG 
ATCAAGGGCC CCACTCGGAC TCCCTATGAA GACGGCCTCT ACCTATTTGA CATCCAGCTT 
CCCAACATCT ACCCTGCGGT GCCCCCCCAC TTTTGCTACC TCTCCCAGTG CAGTGGCCGC 
CTGAACCCCA ACCTGTATGA CAATGGGAAA GTGTGTGTCA GCCTCCTAGG TACCTGGATT 
GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCTAGCCTTC TCCAGGTGCT CATCTCCATC 
CAAGGTCTGA TCCTGGTGAA TGAACCATAC TACAATGAAG CTGGCTTTGA CAGTGACCGG 
GGCCTGCAGG AAGGCTATGA GAACAGTCGC TGCTACAACG AGATGGCATT AATCCGTGTG 
GTGCAGTCCA TGACCCAGTT GGTGCGACGA CCTCCTGAGG TCTTCGAACA GGAGATTCGG 
CAGCACTTCA GTGTGGGTGG CTGGCGGCTG GTGAACCGAA TTGAGTCCTG GCTGGAAACC 
CATGCCATGC AGGAGAGGGC CCAGGTGATG CCCAATGGGG CCCTTAAAGA CAGCAGCTCT 
CTGGAGCCCA TGGCTGCAGC TGAACTCTCA GACTCCGGCC GTGAAGAGCC TGAAGATGTT 
GGGATGGCCC CTGGGGAGGC GTCCCAGGGC TCAGACTCAG AGGGTGGAGC CCAGGGCCCT 
GCCTCAGCCA GCAGAGACCA CACAGAGCAG ACTGAGACAG CCCCTGATGC ATCCGCACCT 
CCCAGCGTTA GACCAAAGAG GAGGAGGAAG AGCTACCGCA GCTTCCTGCC TGAGAAGAGC 
GGTTACCCTG ACATCGGCTT CCCCCTCTTC CCACTCTCCA AGGGTTTCAT CAAGAGCATC 
CGGGGGGTCC TGACACAGTT CCGTGCTGCC CTGCTGGAGG CGGGCATGCC CGAAAGCACA 
GAGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2o ( NM_173755.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_776116.2 Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), mRNA.

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CloneID	OMu21930
Clone ID Related Accession (Same CDS sequence)	NM_173755.3
Accession Version	NM_173755.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Mus musculus(house mouse)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK129431.1, U20780.1 and BC051108.1. On or before Sep 3, 2004 this sequence version replaced XM_109923.5, NM_173755.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATC CCGCAGCGCC CGCACCCGCC CAGGCCCAGG CCGCCGCCGC CCCGACCCCG 
GCCGCAGCCC CCGCCGCCGC CGCCCCGCCG CCCGCGCCCG CTACGGACTC GGCCTCAGGG 
CCGTCCTCCG ACTCCGGCCC CGAAGCAGGC TCACAGCGCC TGCTGTTCTC CCACGACCTG 
GTGTCCGGCC GTTACCGCGG CTCGGTGCAC TTCGGGCTGG TACGCCTCAT CCACGGCGAG 
GACTCGGACT CGGAGGGCGA CGATGACGGC CGCGGCAGCT CGGGCTGCTC CGAGGCCGGG 
GGCGCGGGCC ACGAGGAGGG CCGGGCCAGC CCTCTGCGGC GCGGCTACGT GCGCGTCCAG 
TGGTACCCGG AGGGCGTCAA GCAGCATGTG AAGGAGACCA AGCTGAAACT GGAGGACCGC 
TCTGTGGTGC CCCGGGATGT GGTTCGACAC ATGCGCTCAA CTGACAGCCA GTGTGGCACG 
GTGATAGATG TCAACATTGA CTGTGCCGTC AAGCTCATCG GCACCAACTG CATCATCTAC 
CCTGTCAACA GCAAGGACCT CCAGCACATC TGGCCCTTCA TGTATGGAGA CTATATCGCC 
TATGACTGCT GGCTGGGGAA GGTCTATGAC TTGAAGAACC AGATCATCCT CAAGTTGTCT 
AATGGTGCCA GGTGCTCCAT GAACACAGAA GATGGTGCCA AACTCTACGA TGTCTGCCCT 
CACGTCAGCG ACTCGGGTCT CTTCTTTGAT GACTCTTATG GCTTCTACCC AGGCCAAGTC 
CTTATTGGCC CAGCCAAGAT CTTCTCCAGT GTCCAGTGGC TCTCTGGGGT CAAGCCTGTA 
CTCAGCACCA AGAGCAAGTT CCGTGTGGTG GTGGAGGAGG TGCAGGTTGT AGAACTGAAA 
GTCACGTGGA TTACCAAGAG CTTCTGCCCC GGCGGCACGG ACAGCGTTAG CCCCCCACCT 
TCCATCATCA CTCAGGAAAA CCTAGGCAGG GTGAAGCGTC TCGGATGCTT TGACCATGCT 
CAGCGGCAGC TTGGGGAACG CTGTCTGTAT GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG 
ATTGCCTGGG AATGTCCAGA AAAAAACTGC GCCCAGGGGG AGGGCTCTAT GGCCAAGAAG 
GTGAAGCGTC TGTTGAAGAA GCAAGTTGTG AGAATCATGT CCTGCACCCC AGATACCCAG 
TGTCCTAGGG ACCATTCCAT GGAAGACCCA GACAAGAAAG GGGAAGCCAG AGCCGGGAGT 
GAAATAGGGT CTGCTAGCCC GGAGGAGCAA CCTGATGGAT CAGCCAGCCC CGTGGAGATG 
CAGGATGAGG GGTCGGAGGA GCTTCAGGAG ACATGCGAGC CACTGCCCCC CTTCCTCTTG 
AAGGAGGGTG GAGATGACGG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCGGCT 
GACGATACAG ATGACACCAG CTCTGTGACC TCCTCCGCCA GCTCTACTAC CTCCTCCCAG 
AGTGGCAGTG GCACTGGTCG TAAAAAGAGC ATTCCCTTGT CCATCAAAAA CTTGAAGCGA 
AAACACAAGA GGAAGAAGAA TAAGGTCACC CGTGACTTCA AGCCTGGAGA CAGGGTGGCA 
GTGGAGGTTG TGACCACGAT GACCTCAGCT GATGTGATGT GGCAAGATGG CTCTGTGGAA 
TGCAACATCC GCTCCAACGA CCTTTTCCCT GTGCACCACC TGGACAACAA TGAGTTCTGC 
CCTGGGGACT TCGTGGTGGA CAAGCGAGTC CAGAGCTGCC CAGACCCTGC TGTCTATGGT 
GTGGTGCAGT CTGGGGATCA TGTTGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGCGA 
CCAAGTGGGG ATGACGTGGA GCTCATTGGA GAAGAGGAGG ACGTGAGCGT CTATGACATT 
GCTGACCACC CTGACTTTCG CTTCCGCACA ACTGACATTG TCATCCGCAT TGGTAACACG 
GAGGATGGGG CTCTACCCAA GGAGGATGAG CCCTCAGTGG GCCAGGTGGC CCGAGTGGAT 
GTCAGCAGCA AAGTGGAAGT GGTGTGGGCT GACAACTCGA AAACCATCAT CCTGCCACAG 
CACTTATACA ACATCGAGTC TGAGATTGAG GAGTCTGACT ACGACTCCGT GGAAGGCAGC 
TCCAGTGGGG CCTCCTCGGA TGAGTGGGAG GACGACAGCG ACAGCTGGGA GACCGACAAC 
GGGCTCGTGG ACGATGAGCA CCCCAAGATA GAGGAGCTGG CCGCCATCTT GCCTGCCGAG 
CAGCCCACTG CCCCCGAGGA GGACAAGGGC GTGGTCATCA GTGAGGAGGC GGCCACGGCT 
GCCATCCAGG GCGCCGTGGC CATGGCTGCC CCCGTGGCAG GGCTGATGGA GAAAGCTGGG 
AAGGACGGGC CTCCCAAGAG TTTCCGGGAG CTGAAGGAGG CCATCAAGAT CCTGGAGAGC 
CTCAAGAACA TGACTGTGGA GCAGCTGCTC ACAGGCTCGC CCACCTCGCC CACCGTGGAG 
CCTGAGAAGC CAACCCGAGA GAAGAAGTTT TTGGACGATA TCAAGAAGCT ACAGGAGAAC 
CTCAAGAAGA CCCTGGACAA TGTGGCCATT GCTGAGGAGG AGAAGATGGA GGCAGTACCC 
GACACAGAGC GCAAGGAGGA GAAGCCTGAG GTGCAGTCCC CAGTGAAAGC CGAGTGGCCC 
AGTGAGACAC CTGTGCTCTG TCAGCAGTGC GGTGGCAGGC CTGGCGTCAC CTTCACCAGC 
GCCAAGGGCG AGGTCTTCTC TGTGCTGGAG TTTGCACCCT CAAACCACTC TTTCAAGAAG 
ATTGAGTTCC AGCCCCCGGA AGCCAAGAAG TTCTTCAGCA CAGTGCGGAA GGAGATGGCT 
CTGCTGGCCA CCTCGCTGCC CGATGGCATC ATGGTCAAGA CTTTTGAGGA CAGAATGGAC 
CTCTTCTCTG CACTGATCAA GGGCCCCACT CGGACTCCCT ATGAAGACGG CCTCTACCTA 
TTTGACATCC AGCTTCCCAA CATCTACCCT GCGGTGCCCC CCCACTTTTG CTACCTCTCC 
CAGTGCAGTG GCCGCCTGAA CCCCAACCTG TATGACAATG GGAAAGTGTG TGTCAGCCTC 
CTAGGTACCT GGATTGGAAA GGGGACAGAG AGGTGGACAA GCAAGTCTAG CCTTCTCCAG 
GTGCTCATCT CCATCCAAGG TCTGATCCTG GTGAATGAAC CATACTACAA TGAAGCTGGC 
TTTGACAGTG ACCGGGGCCT GCAGGAAGGC TATGAGAACA GTCGCTGCTA CAACGAGATG 
GCATTAATCC GTGTGGTGCA GTCCATGACC CAGTTGGTGC GACGACCTCC TGAGGTCTTC 
GAACAGGAGA TTCGGCAGCA CTTCAGTGTG GGTGGCTGGC GGCTGGTGAA CCGAATTGAG 
TCCTGGCTGG AAACCCATGC CATGCAGGAG AGGGCCCAGG TGATGCCCAA TGGGGCCCTT 
AAAGACAGCA GCTCTCTGGA GCCCATGGCT GCAGCTGAAC TCTCAGACTC CGGCCGTGAA 
GAGCCTGAAG ATGTTGGGAT GGCCCCTGGG GAGGCGTCCC AGGGCTCAGA CTCAGAGGGT 
GGAGCCCAGG GCCCTGCCTC AGCCAGCAGA GACCACACAG AGCAGACTGA GACAGCCCCT 
GATGCATCCG CACCTCCCAG CGTTAGACCA AAGAGGAGGA GGAAGAGCTA CCGCAGCTTC 
CTGCCTGAGA AGAGCGGTTA CCCTGACATC GGCTTCCCCC TCTTCCCACT CTCCAAGGGT 
TTCATCAAGA GCATCCGGGG GGTCCTGACA CAGTTCCGTG CTGCCCTGCT GGAGGCGGGC 
ATGCCCGAAA GCACAGAGGA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776116.2
CDS	35..3901
Translation	MADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPGEASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTEDK

Target ORF information:

RefSeq Version	NM_173755.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173755.3

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGCC CGCACCCGCC CAGGCCCAGG CCGCCGCCGC CCCGACCCCG 
GCCGCAGCCC CCGCCGCCGC CGCCCCGCCG CCCGCGCCCG CTACGGACTC GGCCTCAGGG 
CCGTCCTCCG ACTCCGGCCC CGAAGCAGGC TCACAGCGCC TGCTGTTCTC CCACGACCTG 
GTGTCCGGCC GTTACCGCGG CTCGGTGCAC TTCGGGCTGG TACGCCTCAT CCACGGCGAG 
GACTCGGACT CGGAGGGCGA CGATGACGGC CGCGGCAGCT CGGGCTGCTC CGAGGCCGGG 
GGCGCGGGCC ACGAGGAGGG CCGGGCCAGC CCTCTGCGGC GCGGCTACGT GCGCGTCCAG 
TGGTACCCGG AGGGCGTCAA GCAGCATGTG AAGGAGACCA AGCTGAAACT GGAGGACCGC 
TCTGTGGTGC CCCGGGATGT GGTTCGACAC ATGCGCTCAA CTGACAGCCA GTGTGGCACG 
GTGATAGATG TCAACATTGA CTGTGCCGTC AAGCTCATCG GCACCAACTG CATCATCTAC 
CCTGTCAACA GCAAGGACCT CCAGCACATC TGGCCCTTCA TGTATGGAGA CTATATCGCC 
TATGACTGCT GGCTGGGGAA GGTCTATGAC TTGAAGAACC AGATCATCCT CAAGTTGTCT 
AATGGTGCCA GGTGCTCCAT GAACACAGAA GATGGTGCCA AACTCTACGA TGTCTGCCCT 
CACGTCAGCG ACTCGGGTCT CTTCTTTGAT GACTCTTATG GCTTCTACCC AGGCCAAGTC 
CTTATTGGCC CAGCCAAGAT CTTCTCCAGT GTCCAGTGGC TCTCTGGGGT CAAGCCTGTA 
CTCAGCACCA AGAGCAAGTT CCGTGTGGTG GTGGAGGAGG TGCAGGTTGT AGAACTGAAA 
GTCACGTGGA TTACCAAGAG CTTCTGCCCC GGCGGCACGG ACAGCGTTAG CCCCCCACCT 
TCCATCATCA CTCAGGAAAA CCTAGGCAGG GTGAAGCGTC TCGGATGCTT TGACCATGCT 
CAGCGGCAGC TTGGGGAACG CTGTCTGTAT GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG 
ATTGCCTGGG AATGTCCAGA AAAAAACTGC GCCCAGGGGG AGGGCTCTAT GGCCAAGAAG 
GTGAAGCGTC TGTTGAAGAA GCAAGTTGTG AGAATCATGT CCTGCACCCC AGATACCCAG 
TGTCCTAGGG ACCATTCCAT GGAAGACCCA GACAAGAAAG GGGAAGCCAG AGCCGGGAGT 
GAAATAGGGT CTGCTAGCCC GGAGGAGCAA CCTGATGGAT CAGCCAGCCC CGTGGAGATG 
CAGGATGAGG GGTCGGAGGA GCTTCAGGAG ACATGCGAGC CACTGCCCCC CTTCCTCTTG 
AAGGAGGGTG GAGATGACGG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCGGCT 
GACGATACAG ATGACACCAG CTCTGTGACC TCCTCCGCCA GCTCTACTAC CTCCTCCCAG 
AGTGGCAGTG GCACTGGTCG TAAAAAGAGC ATTCCCTTGT CCATCAAAAA CTTGAAGCGA 
AAACACAAGA GGAAGAAGAA TAAGGTCACC CGTGACTTCA AGCCTGGAGA CAGGGTGGCA 
GTGGAGGTTG TGACCACGAT GACCTCAGCT GATGTGATGT GGCAAGATGG CTCTGTGGAA 
TGCAACATCC GCTCCAACGA CCTTTTCCCT GTGCACCACC TGGACAACAA TGAGTTCTGC 
CCTGGGGACT TCGTGGTGGA CAAGCGAGTC CAGAGCTGCC CAGACCCTGC TGTCTATGGT 
GTGGTGCAGT CTGGGGATCA TGTTGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGCGA 
CCAAGTGGGG ATGACGTGGA GCTCATTGGA GAAGAGGAGG ACGTGAGCGT CTATGACATT 
GCTGACCACC CTGACTTTCG CTTCCGCACA ACTGACATTG TCATCCGCAT TGGTAACACG 
GAGGATGGGG CTCTACCCAA GGAGGATGAG CCCTCAGTGG GCCAGGTGGC CCGAGTGGAT 
GTCAGCAGCA AAGTGGAAGT GGTGTGGGCT GACAACTCGA AAACCATCAT CCTGCCACAG 
CACTTATACA ACATCGAGTC TGAGATTGAG GAGTCTGACT ACGACTCCGT GGAAGGCAGC 
TCCAGTGGGG CCTCCTCGGA TGAGTGGGAG GACGACAGCG ACAGCTGGGA GACCGACAAC 
GGGCTCGTGG ACGATGAGCA CCCCAAGATA GAGGAGCTGG CCGCCATCTT GCCTGCCGAG 
CAGCCCACTG CCCCCGAGGA GGACAAGGGC GTGGTCATCA GTGAGGAGGC GGCCACGGCT 
GCCATCCAGG GCGCCGTGGC CATGGCTGCC CCCGTGGCAG GGCTGATGGA GAAAGCTGGG 
AAGGACGGGC CTCCCAAGAG TTTCCGGGAG CTGAAGGAGG CCATCAAGAT CCTGGAGAGC 
CTCAAGAACA TGACTGTGGA GCAGCTGCTC ACAGGCTCGC CCACCTCGCC CACCGTGGAG 
CCTGAGAAGC CAACCCGAGA GAAGAAGTTT TTGGACGATA TCAAGAAGCT ACAGGAGAAC 
CTCAAGAAGA CCCTGGACAA TGTGGCCATT GCTGAGGAGG AGAAGATGGA GGCAGTACCC 
GACACAGAGC GCAAGGAGGA GAAGCCTGAG GTGCAGTCCC CAGTGAAAGC CGAGTGGCCC 
AGTGAGACAC CTGTGCTCTG TCAGCAGTGC GGTGGCAGGC CTGGCGTCAC CTTCACCAGC 
GCCAAGGGCG AGGTCTTCTC TGTGCTGGAG TTTGCACCCT CAAACCACTC TTTCAAGAAG 
ATTGAGTTCC AGCCCCCGGA AGCCAAGAAG TTCTTCAGCA CAGTGCGGAA GGAGATGGCT 
CTGCTGGCCA CCTCGCTGCC CGATGGCATC ATGGTCAAGA CTTTTGAGGA CAGAATGGAC 
CTCTTCTCTG CACTGATCAA GGGCCCCACT CGGACTCCCT ATGAAGACGG CCTCTACCTA 
TTTGACATCC AGCTTCCCAA CATCTACCCT GCGGTGCCCC CCCACTTTTG CTACCTCTCC 
CAGTGCAGTG GCCGCCTGAA CCCCAACCTG TATGACAATG GGAAAGTGTG TGTCAGCCTC 
CTAGGTACCT GGATTGGAAA GGGGACAGAG AGGTGGACAA GCAAGTCTAG CCTTCTCCAG 
GTGCTCATCT CCATCCAAGG TCTGATCCTG GTGAATGAAC CATACTACAA TGAAGCTGGC 
TTTGACAGTG ACCGGGGCCT GCAGGAAGGC TATGAGAACA GTCGCTGCTA CAACGAGATG 
GCATTAATCC GTGTGGTGCA GTCCATGACC CAGTTGGTGC GACGACCTCC TGAGGTCTTC 
GAACAGGAGA TTCGGCAGCA CTTCAGTGTG GGTGGCTGGC GGCTGGTGAA CCGAATTGAG 
TCCTGGCTGG AAACCCATGC CATGCAGGAG AGGGCCCAGG TGATGCCCAA TGGGGCCCTT 
AAAGACAGCA GCTCTCTGGA GCCCATGGCT GCAGCTGAAC TCTCAGACTC CGGCCGTGAA 
GAGCCTGAAG ATGTTGGGAT GGCCCCTGGG GAGGCGTCCC AGGGCTCAGA CTCAGAGGGT 
GGAGCCCAGG GCCCTGCCTC AGCCAGCAGA GACCACACAG AGCAGACTGA GACAGCCCCT 
GATGCATCCG CACCTCCCAG CGTTAGACCA AAGAGGAGGA GGAAGAGCTA CCGCAGCTTC 
CTGCCTGAGA AGAGCGGTTA CCCTGACATC GGCTTCCCCC TCTTCCCACT CTCCAAGGGT 
TTCATCAAGA GCATCCGGGG GGTCCTGACA CAGTTCCGTG CTGCCCTGCT GGAGGCGGGC 
ATGCCCGAAA GCACAGAGGA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2o ( XM_006533106.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533169.1 Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.

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CloneID	OMu46179
Clone ID Related Accession (Same CDS sequence)	XM_006533106.2 , XM_006533106.1
Accession Version	XM_006533106.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGCC CGCACCCGCC CAGGCCCAGG CCGCCGCCGC CCCGACCCCG 
GCCGCAGCCC CCGCCGCCGC CGCCCCGCCG CCCGCGCCCG CTACGGACTC GGCCTCAGGG 
CCGTCCTCCG ACTCCGGCCC CGAAGCAGGC TCACAGCGCC TGCTGTTCTC CCACGACCTG 
GTGTCCGGCC GTTACCGCGG CTCGGTGCAC TTCGGGCTGG TACGCCTCAT CCACGGCGAG 
GACTCGGACT CGGAGGGCGA CGATGACGGC CGCGGCAGCT CGGGCTGCTC CGAGGCCGGG 
GGCGCGGGCC ACGAGGAGGG CCGGGCCAGC CCTCTGCGGC GCGGCTACGT GCGCGTCCAG 
TGGTACCCGG AGGGCGTCAA GCAGCATGTG AAGGAGACCA AGCTGAAACT GGAGGACCGC 
TCTGTGGTGC CCCGGGATGT GGTTCGACAC ATGCGCTCAA CTGACAGCCA GTGTGGCACG 
GTGATAGATG TCAACATTGA CTGTGCCGTC AAGCTCATCG GCACCAACTG CATCATCTAC 
CCTGTCAACA GCAAGGACCT CCAGCACATC TGGCCCTTCA TGTATGGAGA CTATATCGCC 
TATGACTGCT GGCTGGGGAA GGTCTATGAC TTGAAGAACC AGATCATCCT CAAGTTGTCT 
AATGGTGCCA GGTGCTCCAT GAACACAGAA GATGGTGCCA AACTCTACGA TGTCTGCCCT 
CACGTCAGCG ACTCGGGTCT CTTCTTTGAT GACTCTTATG GCTTCTACCC AGGCCAAGTC 
CTTATTGGCC CAGCCAAGAT CTTCTCCAGT GTCCAGTGGC TCTCTGGGGT CAAGCCTGTA 
CTCAGCACCA AGAGCAAGTT CCGTGTGGTG GTGGAGGAGG TGCAGGTTGT AGAACTGAAA 
GTCACGTGGA TTACCAAGAG CTTCTGCCCC GGCGGCACGG ACAGCGTTAG CCCCCCACCT 
TCCATCATCA CTCAGGAAAA CCTAGGCAGG GTGAAGCGTC TCGGATGCTT TGACCATGCT 
CAGCGGCAGC TTGGGGAACG CTGTCTGTAT GTCTTCCCAG CCAAGGTGAA GCGTCTGTTG 
AAGAAGCAAG TTGTGAGAAT CATGTCCTGC ACCCCAGATA CCCAGTGTCC TAGGGACCAT 
TCCATGGAAG ACCCAGACAA GAAAGGGGAA GCCAGAGCCG GGAGTGAAAT AGGGTCTGCT 
AGCCCGGAGG AGCAACCTGA TGGATCAGCC AGCCCCGTGG AGATGCAGGA TGAGGGGTCG 
GAGGAGCTTC AGGAGACATG CGAGCCACTG CCCCCCTTCC TCTTGAAGGA GGGTGGAGAT 
GACGGGCTGC ACTCGGCAGA GCAGGACGCA GATGATGAGG CGGCTGACGA TACAGATGAC 
ACCAGCTCTG TGACCTCCTC CGCCAGCTCT ACTACCTCCT CCCAGAGTGG CAGTGGCACT 
GGTCGTAAAA AGAGCATTCC CTTGTCCATC AAAAACTTGA AGCGAAAACA CAAGAGGAAG 
AAGAATAAGG TCACCCGTGA CTTCAAGCCT GGAGACAGGG TGGCAGTGGA GGTTGTGACC 
ACGATGACCT CAGCTGATGT GATGTGGCAA GATGGCTCTG TGGAATGCAA CATCCGCTCC 
AACGACCTTT TCCCTGTGCA CCACCTGGAC AACAATGAGT TCTGCCCTGG GGACTTCGTG 
GTGGACAAGC GAGTCCAGAG CTGCCCAGAC CCTGCTGTCT ATGGTGTGGT GCAGTCTGGG 
GATCATGTTG GCCGTACCTG CATGGTGAAG TGGTTCAAGC TGCGACCAAG TGGGGATGAC 
GTGGAGCTCA TTGGAGAAGA GGAGGACGTG AGCGTCTATG ACATTGCTGA CCACCCTGAC 
TTTCGCTTCC GCACAACTGA CATTGTCATC CGCATTGGTA ACACGGAGGA TGGGGCTCTA 
CCCAAGGAGG ATGAGCCCTC AGTGGGCCAG GTGGCCCGAG TGGATGTCAG CAGCAAAGTG 
GAAGTGGTGT GGGCTGACAA CTCGAAAACC ATCATCCTGC CACAGCACTT ATACAACATC 
GAGTCTGAGA TTGAGGAGTC TGACTACGAC TCCGTGGAAG GCAGCTCCAG TGGGGCCTCC 
TCGGATGAGT GGGAGGACGA CAGCGACAGC TGGGAGACCG ACAACGGGCT CGTGGACGAT 
GAGCACCCCA AGATAGAGGA GCTGGCCGCC ATCTTGCCTG CCGAGCAGCC CACTGCCCCC 
GAGGAGGACA AGGGCGTGGT CATCAGTGAG GAGGCGGCCA CGGCTGCCAT CCAGGGCGCC 
GTGGCCATGG CTGCCCCCGT GGCAGGGCTG ATGGAGAAAG CTGGGAAGGA CGGGCCTCCC 
AAGAGTTTCC GGGAGCTGAA GGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT 
GTGGAGCAGC TGCTCACAGG CTCGCCCACC TCGCCCACCG TGGAGCCTGA GAAGCCAACC 
CGAGAGAAGA AGTTTTTGGA CGATATCAAG AAGCTACAGG AGAACCTCAA GAAGACCCTG 
GACAATGTGG CCATTGCTGA GGAGGAGAAG ATGGAGGCAG TACCCGACAC AGAGCGCAAG 
GAGGAGAAGC CTGAGGTGCA GTCCCCAGTG AAAGCCGAGT GGCCCAGTGA GACACCTGTG 
CTCTGTCAGC AGTGCGGTGG CAGGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC 
TTCTCTGTGC TGGAGTTTGC ACCCTCAAAC CACTCTTTCA AGAAGATTGA GTTCCAGCCC 
CCGGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCTCTGCT GGCCACCTCG 
CTGCCCGATG GCATCATGGT CAAGACTTTT GAGGACAGAA TGGACCTCTT CTCTGCACTG 
ATCAAGGGCC CCACTCGGAC TCCCTATGAA GACGGCCTCT ACCTATTTGA CATCCAGCTT 
CCCAACATCT ACCCTGCGGT GCCCCCCCAC TTTTGCTACC TCTCCCAGTG CAGTGGCCGC 
CTGAACCCCA ACCTGTATGA CAATGGGAAA GTGTGTGTCA GCCTCCTAGG TACCTGGATT 
GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCTAGCCTTC TCCAGGTGCT CATCTCCATC 
CAAGGTCTGA TCCTGGTGAA TGAACCATAC TACAATGAAG CTGGCTTTGA CAGTGACCGG 
GGCCTGCAGG AAGGCTATGA GAACAGTCGC TGCTACAACG AGATGGCATT AATCCGTGTG 
GTGCAGTCCA TGACCCAGTT GGTGCGACGA CCTCCTGAGG TCTTCGAACA GGAGATTCGG 
CAGCACTTCA GTGTGGGTGG CTGGCGGCTG GTGAACCGAA TTGAGTCCTG GCTGGAAACC 
CATGCCATGC AGGAGAGGGC CCAGGTGATG CCCAATGGGG CCCTTAAAGA CAGCAGCTCT 
CTGGAGCCCA TGGCTGCAGC TGAACTCTCA GACTCCGGCC GTGAAGAGCC TGAAGATGTT 
GGGATGGCCC CTGGGGAGGC GTCCCAGGGC TCAGACTCAG AGGGTGGAGC CCAGGGCCCT 
GCCTCAGCCA GCAGAGACCA CACAGAGCAG ACTGAGACAG CCCCTGATGC ATCCGCACCT 
CCCAGCGTTA GACCAAAGAG GAGGAGGAAG AGCTACCGCA GCTTCCTGCC TGAGAAGAGC 
GGTTACCCTG ACATCGGCTT CCCCCTCTTC CCACTCTCCA AGGGTTTCAT CAAGAGCATC 
CGGGGGGTCC TGACACAGTT CCGTGCTGCC CTGCTGGAGG CGGGCATGCC CGAAAGCACA 
GAGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533169.1
CDS	64..3855
Translation	MADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPGEASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTEDK

Target ORF information:

RefSeq Version	XM_006533106.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533106.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGCC CGCACCCGCC CAGGCCCAGG CCGCCGCCGC CCCGACCCCG 
GCCGCAGCCC CCGCCGCCGC CGCCCCGCCG CCCGCGCCCG CTACGGACTC GGCCTCAGGG 
CCGTCCTCCG ACTCCGGCCC CGAAGCAGGC TCACAGCGCC TGCTGTTCTC CCACGACCTG 
GTGTCCGGCC GTTACCGCGG CTCGGTGCAC TTCGGGCTGG TACGCCTCAT CCACGGCGAG 
GACTCGGACT CGGAGGGCGA CGATGACGGC CGCGGCAGCT CGGGCTGCTC CGAGGCCGGG 
GGCGCGGGCC ACGAGGAGGG CCGGGCCAGC CCTCTGCGGC GCGGCTACGT GCGCGTCCAG 
TGGTACCCGG AGGGCGTCAA GCAGCATGTG AAGGAGACCA AGCTGAAACT GGAGGACCGC 
TCTGTGGTGC CCCGGGATGT GGTTCGACAC ATGCGCTCAA CTGACAGCCA GTGTGGCACG 
GTGATAGATG TCAACATTGA CTGTGCCGTC AAGCTCATCG GCACCAACTG CATCATCTAC 
CCTGTCAACA GCAAGGACCT CCAGCACATC TGGCCCTTCA TGTATGGAGA CTATATCGCC 
TATGACTGCT GGCTGGGGAA GGTCTATGAC TTGAAGAACC AGATCATCCT CAAGTTGTCT 
AATGGTGCCA GGTGCTCCAT GAACACAGAA GATGGTGCCA AACTCTACGA TGTCTGCCCT 
CACGTCAGCG ACTCGGGTCT CTTCTTTGAT GACTCTTATG GCTTCTACCC AGGCCAAGTC 
CTTATTGGCC CAGCCAAGAT CTTCTCCAGT GTCCAGTGGC TCTCTGGGGT CAAGCCTGTA 
CTCAGCACCA AGAGCAAGTT CCGTGTGGTG GTGGAGGAGG TGCAGGTTGT AGAACTGAAA 
GTCACGTGGA TTACCAAGAG CTTCTGCCCC GGCGGCACGG ACAGCGTTAG CCCCCCACCT 
TCCATCATCA CTCAGGAAAA CCTAGGCAGG GTGAAGCGTC TCGGATGCTT TGACCATGCT 
CAGCGGCAGC TTGGGGAACG CTGTCTGTAT GTCTTCCCAG CCAAGGTGAA GCGTCTGTTG 
AAGAAGCAAG TTGTGAGAAT CATGTCCTGC ACCCCAGATA CCCAGTGTCC TAGGGACCAT 
TCCATGGAAG ACCCAGACAA GAAAGGGGAA GCCAGAGCCG GGAGTGAAAT AGGGTCTGCT 
AGCCCGGAGG AGCAACCTGA TGGATCAGCC AGCCCCGTGG AGATGCAGGA TGAGGGGTCG 
GAGGAGCTTC AGGAGACATG CGAGCCACTG CCCCCCTTCC TCTTGAAGGA GGGTGGAGAT 
GACGGGCTGC ACTCGGCAGA GCAGGACGCA GATGATGAGG CGGCTGACGA TACAGATGAC 
ACCAGCTCTG TGACCTCCTC CGCCAGCTCT ACTACCTCCT CCCAGAGTGG CAGTGGCACT 
GGTCGTAAAA AGAGCATTCC CTTGTCCATC AAAAACTTGA AGCGAAAACA CAAGAGGAAG 
AAGAATAAGG TCACCCGTGA CTTCAAGCCT GGAGACAGGG TGGCAGTGGA GGTTGTGACC 
ACGATGACCT CAGCTGATGT GATGTGGCAA GATGGCTCTG TGGAATGCAA CATCCGCTCC 
AACGACCTTT TCCCTGTGCA CCACCTGGAC AACAATGAGT TCTGCCCTGG GGACTTCGTG 
GTGGACAAGC GAGTCCAGAG CTGCCCAGAC CCTGCTGTCT ATGGTGTGGT GCAGTCTGGG 
GATCATGTTG GCCGTACCTG CATGGTGAAG TGGTTCAAGC TGCGACCAAG TGGGGATGAC 
GTGGAGCTCA TTGGAGAAGA GGAGGACGTG AGCGTCTATG ACATTGCTGA CCACCCTGAC 
TTTCGCTTCC GCACAACTGA CATTGTCATC CGCATTGGTA ACACGGAGGA TGGGGCTCTA 
CCCAAGGAGG ATGAGCCCTC AGTGGGCCAG GTGGCCCGAG TGGATGTCAG CAGCAAAGTG 
GAAGTGGTGT GGGCTGACAA CTCGAAAACC ATCATCCTGC CACAGCACTT ATACAACATC 
GAGTCTGAGA TTGAGGAGTC TGACTACGAC TCCGTGGAAG GCAGCTCCAG TGGGGCCTCC 
TCGGATGAGT GGGAGGACGA CAGCGACAGC TGGGAGACCG ACAACGGGCT CGTGGACGAT 
GAGCACCCCA AGATAGAGGA GCTGGCCGCC ATCTTGCCTG CCGAGCAGCC CACTGCCCCC 
GAGGAGGACA AGGGCGTGGT CATCAGTGAG GAGGCGGCCA CGGCTGCCAT CCAGGGCGCC 
GTGGCCATGG CTGCCCCCGT GGCAGGGCTG ATGGAGAAAG CTGGGAAGGA CGGGCCTCCC 
AAGAGTTTCC GGGAGCTGAA GGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT 
GTGGAGCAGC TGCTCACAGG CTCGCCCACC TCGCCCACCG TGGAGCCTGA GAAGCCAACC 
CGAGAGAAGA AGTTTTTGGA CGATATCAAG AAGCTACAGG AGAACCTCAA GAAGACCCTG 
GACAATGTGG CCATTGCTGA GGAGGAGAAG ATGGAGGCAG TACCCGACAC AGAGCGCAAG 
GAGGAGAAGC CTGAGGTGCA GTCCCCAGTG AAAGCCGAGT GGCCCAGTGA GACACCTGTG 
CTCTGTCAGC AGTGCGGTGG CAGGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC 
TTCTCTGTGC TGGAGTTTGC ACCCTCAAAC CACTCTTTCA AGAAGATTGA GTTCCAGCCC 
CCGGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCTCTGCT GGCCACCTCG 
CTGCCCGATG GCATCATGGT CAAGACTTTT GAGGACAGAA TGGACCTCTT CTCTGCACTG 
ATCAAGGGCC CCACTCGGAC TCCCTATGAA GACGGCCTCT ACCTATTTGA CATCCAGCTT 
CCCAACATCT ACCCTGCGGT GCCCCCCCAC TTTTGCTACC TCTCCCAGTG CAGTGGCCGC 
CTGAACCCCA ACCTGTATGA CAATGGGAAA GTGTGTGTCA GCCTCCTAGG TACCTGGATT 
GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCTAGCCTTC TCCAGGTGCT CATCTCCATC 
CAAGGTCTGA TCCTGGTGAA TGAACCATAC TACAATGAAG CTGGCTTTGA CAGTGACCGG 
GGCCTGCAGG AAGGCTATGA GAACAGTCGC TGCTACAACG AGATGGCATT AATCCGTGTG 
GTGCAGTCCA TGACCCAGTT GGTGCGACGA CCTCCTGAGG TCTTCGAACA GGAGATTCGG 
CAGCACTTCA GTGTGGGTGG CTGGCGGCTG GTGAACCGAA TTGAGTCCTG GCTGGAAACC 
CATGCCATGC AGGAGAGGGC CCAGGTGATG CCCAATGGGG CCCTTAAAGA CAGCAGCTCT 
CTGGAGCCCA TGGCTGCAGC TGAACTCTCA GACTCCGGCC GTGAAGAGCC TGAAGATGTT 
GGGATGGCCC CTGGGGAGGC GTCCCAGGGC TCAGACTCAG AGGGTGGAGC CCAGGGCCCT 
GCCTCAGCCA GCAGAGACCA CACAGAGCAG ACTGAGACAG CCCCTGATGC ATCCGCACCT 
CCCAGCGTTA GACCAAAGAG GAGGAGGAAG AGCTACCGCA GCTTCCTGCC TGAGAAGAGC 
GGTTACCCTG ACATCGGCTT CCCCCTCTTC CCACTCTCCA AGGGTTTCAT CAAGAGCATC 
CGGGGGGTCC TGACACAGTT CCGTGCTGCC CTGCTGGAGG CGGGCATGCC CGAAAGCACA 
GAGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UBE2O remodels the proteome during terminal erythroid differentiation.
Science (New York, N.Y.)357(6350)(2017 08)
Nguyen AT,Prado MA,Schmidt PJ,Sendamarai AK,Wilson-Grady JT,Min M,Campagna DR,Tian G,Shi Y,Dederer V,Kawan M,Kuehnle N,Paulo JA,Yao Y,Weiss MJ,Justice MJ,Gygi SP,Fleming MD,Finley D

A UBE2O-AMPK?2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.
Cancer cell31(2)208-224(2017 Feb)
Vila IK,Yao Y,Kim G,Xia W,Kim H,Kim SJ,Park MK,Hwang JP,Gonz?lez-Billalabeitia E,Hung MC,Song SJ,Song MS

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Ube2o cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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