Trim47 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim47
Entrez Gene ID	217333
Full Name	tripartite motif-containing 47
Synonyms	2210023F24Rik
General protein information	Preferred Names tripartite motif-containing 47 Names tripartite motif-containing protein 47 tripartite motif protein 47
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001205081.1	NP_001192010.1	E3 ubiquitin-protein ligase TRIM47 isoform 1
NM_172570.5	NP_766158.3	E3 ubiquitin-protein ligase TRIM47 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Trim47	NP_001103055.1
Gallus gallus (chicken)	TRIM47	XP_003642373.1
Bos taurus (cattle)	TRIM47	NP_001180126.1
Mus musculus (house mouse)	Trim47	NP_001192010.1
	TRIM47	XP_001101126.1
Homo sapiens (human)	TRIM47	NP_258411.2
Pan troglodytes (chimpanzee)	TRIM47	XP_001147664.1
Canis lupus familiaris (dog)	TRIM47	XP_851873.2

Gene Ontology
Bibliography

Related articles in PubMed

The deubiquitinating enzyme cylindromatosis mitigates nonalcoholic steatohepatitis.
Ji YX, Huang Z, Yang X, Wang X, Zhao LP, Wang PX, Zhang XJ, Alves-Bezerra M, Cai L, Zhang P, Lu YX, Bai L, Gao MM, Zhao H, Tian S, Wang Y, Huang ZX, Zhu XY, Zhang Y, Gong J, She ZG, Li F, Cohen DE, Li H
Nature medicine(2018 Jan)

Expression of LIM-homeodomain transcription factors in the developing and mature mouse retina.
Balasubramanian R, Bui A, Ding Q, Gan L
Gene expression patterns : GEP14(1)1-8(2014 Jan)

Elucidation of the transcription network governing mammalian sex determination by exploiting strain-specific susceptibility to sex reversal.
Munger SC, Aylor DL, Syed HA, Magwene PM, Threadgill DW, Capel B
Genes & development23(21)2521-36(2009 Nov)

A multipotent progenitor domain guides pancreatic organogenesis.
Zhou Q, Law AC, Rajagopal J, Anderson WJ, Gray PA, Melton DA
Developmental cell13(1)103-14(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trim47 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim47 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02766	NM_001205081.1 Latest version!	Mus musculus tripartite motif-containing 47 (Trim47), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu03697	NM_172570.5 Latest version!	Mus musculus tripartite motif-containing 47 (Trim47), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$328.30 $469.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim47 ( NM_001205081.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001192010.1 Mus musculus tripartite motif-containing 47 (Trim47), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02766
Clone ID Related Accession (Same CDS sequence)	NM_001205081.1
Accession Version	NM_001205081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM47 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL607108.17, BC145059.1 and BG065693.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC145059.1, BU517374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGGACGGCA GCGGGCCTTT CAGCTGCCCC ATCTGCCTGG AACCGCTCCG GGAGCCGGTG 
ACACTACCCT GCGGCCACAA CTTCTGCCTC GCTTGCCTGG GCGCTCTCTG GCCGCACCGG 
AGCGCGGGCG GCACCGGTGG TTCCGGAGGT CCAGCTCGCT GTCCACTTTG TCAGGAGCCT 
TTCCCCGACG GCCTGCAGCT CCGCAAGAAC CACACGCTGT CCGAGTTGCT GCAGCTCCGC 
CAGGGCTCGG TCCCGGGACC CATGTCCGCC CCGGCTTCAG GATCGACCCG GGGCGCCACG 
CCCGAGCCCT CCGCGCCCAG CGCACCACCT CCGGCTCCGG AACCATCGGC TCCCTGCGCG 
CCGGAGCAGT GGCCCGCGGG TGAGGAGCCG GTGCGCTGCG ACGCGTGCCC CGAGGGCGCC 
GCCCTGCCCG CCGCGCTCTC TTGCCTCTCC TGCCTCGCCT CCTTCTGCTC CGCGCATCTG 
GCCCCCCACG AACGCAGCCC CGCCCTGCGC GGCCACCGCC TGGTGCCCCC GCTGCGCCGA 
CTTGAGGAGA GCCTGTGTCC GCGCCACCTG CGGCCTCTGG AGCGCTACTG TCGCGTGGAG 
CGTGTGTGCC TTTGCGAGGC CTGTGCCACT CAGGACCATC GCGGCCACGA GCTCGTGCCG 
CTGGAGCAGG AGCGCGCGCT TCAGGAGGTG GAGCAGTCCA AAGTCCTGAG CGCTGCGGAG 
GACCGCATGG ATGAACTGGG TGCCGGCATC GCACAGTCCC GCCGTACTGT GGCCCTCATC 
AAGAGTGCAG CTGTGGCAGA GCGGGAGAGG GTGAGCCAGA TGTTTGCCGA GGCCACAGCC 
ACCCTTCAGA GTTTCCAGAA CGAGGTGATG GGGTTCATCG AAGAGGGAGA GGCCACAATG 
TTGGGCCGGT CCCAGGGTGA CCTGCGTAGA CAAGAGGAAC AGCGCAGCCG CCTAAGCAAG 
GCACGGCACA ACCTCGGTCA GGTTCCAGAG GCGGATTCAG TCAGCTTTCT CCAGGAGCTC 
CTGGCACTAC GGCTGGCCCT GGAGGAGGGG TGCGGCCCCG GGCCTGGGCC CCCCAGGGAA 
CTCAGCTTCA CCAAGTCCTC CCAGGTGGTC AAAGCGGTGA GAGACACCCT CATCTCAGCC 
TGTGCCAGCC AGTGGGAGCA GCTGCGGGGG CTGGGCAGCA ATGAGGATGG GCTACAGAAA 
CTCGGCTCAG AAGCAGATGT GGAGTCCCAG GACCCTGACA GTACCAGCCT GCTGGAGAGT 
GAGGCCCCCA GGGATTACTT CCTCAAGTTT GCCTACATCG TGGACCTGGA CAGCGACACC 
GCGGACAAGT TCCTGCAGCT TTTTGGAACT AAAGGTGTCA AGAGAGTACT GTGTCCCATC 
AACTACCCGG AGTCACCCAC CCGCTTCACA CACTGCGAGC AGGTGCTAGG AGAGGGCGCC 
CTGGACCGGG GCACCTACTA CTGGGAGGTG GAGATCATCG AAGGATGGGT CAGTGTGGGA 
GTCATGGCCG AGGGCTTCTC CCCGCAAGAG CCCTATGACC GGGGCCGGCT GGGCCGCAAT 
GCACACTCAT GCTGTCTGCA GTGGAATGGG CGTGGCTTCT CAGTCTGGTT CTGCGGGCTG 
GAGGCCCCGC TACCCCATGC CTTTTCGCCT ACAGTCGGGG TCTGCCTAGA GTATGCAGAC 
CATGCCCTGG CCTTCTATGC TGTCCGAGAC GGAAAGCTGA GCCTTCTTCG GAGGCTCAAA 
GCCTCCCGGC CTCGCCGCAG CGGTGCTCTG GCCTCCCCCA CTGACCCTTT TCAGAGTCGC 
CTGGACAGCC ATTTTTCCGG GCTCTTCAAC CACAGGCTCA AGCCTGCCTT CTTCCTGGAG 
AGTGTGGACG CCCATCTGCA GATTGGACCT CTCAAGAAAT CGTGCATAAC CGTGCTGAAG 
AGGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192010.1
CDS	16..1944
Translation	MDGSGPFSCPICLEPLREPVTLPCGHNFCLACLGALWPHRSAGGTGGSGGPARCPLCQEPFPDGLQLRKNHTLSELLQLRQGSVPGPMSAPASGSTRGATPEPSAPSAPPPAPEPSAPCAPEQWPAGEEPVRCDACPEGAALPAALSCLSCLASFCSAHLAPHERSPALRGHRLVPPLRRLEESLCPRHLRPLERYCRVERVCLCEACATQDHRGHELVPLEQERALQEVEQSKVLSAAEDRMDELGAGIAQSRRTVALIKSAAVAERERVSQMFAEATATLQSFQNEVMGFIEEGEATMLGRSQGDLRRQEEQRSRLSKARHNLGQVPEADSVSFLQELLALRLALEEGCGPGPGPPRELSFTKSSQVVKAVRDTLISACASQWEQLRGLGSNEDGLQKLGSEADVESQDPDSTSLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPESPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSVGVMAEGFSPQEPYDRGRLGRNAHSCCLQWNGRGFSVWFCGLEAPLPHAFSPTVGVCLEYADHALAFYAVRDGKLSLLRRLKASRPRRSGALASPTDPFQSRLDSHFSGLFNHRLKPAFFLESVDAHLQIGPLKKSCITVLKRR

Target ORF information:

RefSeq Version	NM_001205081.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 47 (Trim47), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205081.1

ORF Insert Sequence:

ATGGACGGCA GCGGGCCTTT CAGCTGCCCC ATCTGCCTGG AACCGCTCCG GGAGCCGGTG 
ACACTACCCT GCGGCCACAA CTTCTGCCTC GCTTGCCTGG GCGCTCTCTG GCCGCACCGG 
AGCGCGGGCG GCACCGGTGG TTCCGGAGGT CCAGCTCGCT GTCCACTTTG TCAGGAGCCT 
TTCCCCGACG GCCTGCAGCT CCGCAAGAAC CACACGCTGT CCGAGTTGCT GCAGCTCCGC 
CAGGGCTCGG TCCCGGGACC CATGTCCGCC CCGGCTTCAG GATCGACCCG GGGCGCCACG 
CCCGAGCCCT CCGCGCCCAG CGCACCACCT CCGGCTCCGG AACCATCGGC TCCCTGCGCG 
CCGGAGCAGT GGCCCGCGGG TGAGGAGCCG GTGCGCTGCG ACGCGTGCCC CGAGGGCGCC 
GCCCTGCCCG CCGCGCTCTC TTGCCTCTCC TGCCTCGCCT CCTTCTGCTC CGCGCATCTG 
GCCCCCCACG AACGCAGCCC CGCCCTGCGC GGCCACCGCC TGGTGCCCCC GCTGCGCCGA 
CTTGAGGAGA GCCTGTGTCC GCGCCACCTG CGGCCTCTGG AGCGCTACTG TCGCGTGGAG 
CGTGTGTGCC TTTGCGAGGC CTGTGCCACT CAGGACCATC GCGGCCACGA GCTCGTGCCG 
CTGGAGCAGG AGCGCGCGCT TCAGGAGGTG GAGCAGTCCA AAGTCCTGAG CGCTGCGGAG 
GACCGCATGG ATGAACTGGG TGCCGGCATC GCACAGTCCC GCCGTACTGT GGCCCTCATC 
AAGAGTGCAG CTGTGGCAGA GCGGGAGAGG GTGAGCCAGA TGTTTGCCGA GGCCACAGCC 
ACCCTTCAGA GTTTCCAGAA CGAGGTGATG GGGTTCATCG AAGAGGGAGA GGCCACAATG 
TTGGGCCGGT CCCAGGGTGA CCTGCGTAGA CAAGAGGAAC AGCGCAGCCG CCTAAGCAAG 
GCACGGCACA ACCTCGGTCA GGTTCCAGAG GCGGATTCAG TCAGCTTTCT CCAGGAGCTC 
CTGGCACTAC GGCTGGCCCT GGAGGAGGGG TGCGGCCCCG GGCCTGGGCC CCCCAGGGAA 
CTCAGCTTCA CCAAGTCCTC CCAGGTGGTC AAAGCGGTGA GAGACACCCT CATCTCAGCC 
TGTGCCAGCC AGTGGGAGCA GCTGCGGGGG CTGGGCAGCA ATGAGGATGG GCTACAGAAA 
CTCGGCTCAG AAGCAGATGT GGAGTCCCAG GACCCTGACA GTACCAGCCT GCTGGAGAGT 
GAGGCCCCCA GGGATTACTT CCTCAAGTTT GCCTACATCG TGGACCTGGA CAGCGACACC 
GCGGACAAGT TCCTGCAGCT TTTTGGAACT AAAGGTGTCA AGAGAGTACT GTGTCCCATC 
AACTACCCGG AGTCACCCAC CCGCTTCACA CACTGCGAGC AGGTGCTAGG AGAGGGCGCC 
CTGGACCGGG GCACCTACTA CTGGGAGGTG GAGATCATCG AAGGATGGGT CAGTGTGGGA 
GTCATGGCCG AGGGCTTCTC CCCGCAAGAG CCCTATGACC GGGGCCGGCT GGGCCGCAAT 
GCACACTCAT GCTGTCTGCA GTGGAATGGG CGTGGCTTCT CAGTCTGGTT CTGCGGGCTG 
GAGGCCCCGC TACCCCATGC CTTTTCGCCT ACAGTCGGGG TCTGCCTAGA GTATGCAGAC 
CATGCCCTGG CCTTCTATGC TGTCCGAGAC GGAAAGCTGA GCCTTCTTCG GAGGCTCAAA 
GCCTCCCGGC CTCGCCGCAG CGGTGCTCTG GCCTCCCCCA CTGACCCTTT TCAGAGTCGC 
CTGGACAGCC ATTTTTCCGG GCTCTTCAAC CACAGGCTCA AGCCTGCCTT CTTCCTGGAG 
AGTGTGGACG CCCATCTGCA GATTGGACCT CTCAAGAAAT CGTGCATAAC CGTGCTGAAG 
AGGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim47 ( NM_172570.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766158.3 Mus musculus tripartite motif-containing 47 (Trim47), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu03697
Clone ID Related Accession (Same CDS sequence)	NM_172570.5
Accession Version	NM_172570.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM47 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL607108.17, BC100317.1 and BG065693.2. On Mar 22, 2011 this sequence version replaced NM_172570.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC100317.1, AK031572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGGACGGCA GCGGGCCTTT CAGCTGCCCC ATCTGCCTGG AACCGCTCCG GGAGCCGGTG 
ACACTACCCT GCGGCCACAA CTTCTGCCTC GCTTGCCTGG GCGCTCTCTG GCCGCACCGG 
AGCGCGGGCG GCACCGGTGG TTCCGGAGGT CCAGCTCGCT GTCCACTTTG TCAGGAGCCT 
TTCCCCGACG GCCTGCAGCT CCGCAAGAAC CACACGCTGT CCGAGTTGCT GCAGCTCCGC 
CAGGGCTCGG TCCCGGGACC CATGTCCGCC CCGGCTTCAG GATCGACCCG GGGCGCCACG 
CCCGAGCCCT CCGCGCCCAG CGCACCACCT CCGGCTCCGG AACCATCGGC TCCCTGCGCG 
CCGGAGCAGT GGCCCGCGGG TGAGGAGCCG GTGCGCTGCG ACGCGTGCCC CGAGGGCGCC 
GCCCTGCCCG CCGCGCTCTC TTGCCTCTCC TGCCTCGCCT CCTTCTGCTC CGCGCATCTG 
GCCCCCCACG AACGCAGCCC CGCCCTGCGC GGCCACCGCC TGGTGCCCCC GCTGCGCCGA 
CTTGAGGAGA GCCTGTGTCC GCGCCACCTG CGGCCTCTGG AGCGCTACTG TCGCGTGGAG 
CGTGTGTGCC TTTGCGAGGC CTGTGCCACT CAGGACCATC GCGGCCACGA GCTCGTGCCG 
CTGGAGCAGG AGCGCGCGCT TCAGGAGGTG GAGCAGTCCA AAGTCCTGAG CGCTGCGGAG 
GACCGCATGG ATGAACTGGG TGCCGGCATC GCACAGTCCC GCCGTACTGT GGCCCTCATC 
AAGAGTGCAG CTGTGGCAGA GCGGGAGAGG GTGAGCCAGA TGTTTGCCGA GGCCACAGCC 
ACCCTTCAGA GTTTCCAGAA CGAGGTGATG GGGTTCATCG AAGAGGGAGA GGCCACAATG 
TTGGGCCGGT CCCAGGGTGA CCTGCGTAGA CAAGAGGAAC AGCGCAGCCG CCTAAGCAAG 
GCACGGCACA ACCTCGGTCA GGTTCCAGAG GCGGATTCAG TCAGCTTTCT CCAGGAGCTC 
CTGGCACTAC GGCTGGCCCT GGAGGAGGGG TGCGGCCCCG GGCCTGGGCC CCCCAGGGAA 
CTCAGCTTCA CCAAGTCCTC CCAGGTGGTC AAAGCGGTGA GAGACACCCT CATCTCAGCC 
TGTGCCAGCC AGTGGGAGCA GCTGCGGGGG CTGGGCAGCA ATGAGGATGG GCTACAGAAA 
CTCGGCTCAG AAGATGTGGA GTCCCAGGAC CCTGACAGTA CCAGCCTGCT GGAGAGTGAG 
GCCCCCAGGG ATTACTTCCT CAAGTTTGCC TACATCGTGG ACCTGGACAG CGACACCGCG 
GACAAGTTCC TGCAGCTTTT TGGAACTAAA GGTGTCAAGA GAGTACTGTG TCCCATCAAC 
TACCCGGAGT CACCCACCCG CTTCACACAC TGCGAGCAGG TGCTAGGAGA GGGCGCCCTG 
GACCGGGGCA CCTACTACTG GGAGGTGGAG ATCATCGAAG GATGGGTCAG TGTGGGAGTC 
ATGGCCGAGG GCTTCTCCCC GCAAGAGCCC TATGACCGGG GCCGGCTGGG CCGCAATGCA 
CACTCATGCT GTCTGCAGTG GAATGGGCGT GGCTTCTCAG TCTGGTTCTG CGGGCTGGAG 
GCCCCGCTAC CCCATGCCTT TTCGCCTACA GTCGGGGTCT GCCTAGAGTA TGCAGACCAT 
GCCCTGGCCT TCTATGCTGT CCGAGACGGA AAGCTGAGCC TTCTTCGGAG GCTCAAAGCC 
TCCCGGCCTC GCCGCAGCGG TGCTCTGGCC TCCCCCACTG ACCCTTTTCA GAGTCGCCTG 
GACAGCCATT TTTCCGGGCT CTTCAACCAC AGGCTCAAGC CTGCCTTCTT CCTGGAGAGT 
GTGGACGCCC ATCTGCAGAT TGGACCTCTC AAGAAATCGT GCATAACCGT GCTGAAGAGG 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766158.3
CDS	16..1941
Translation	MDGSGPFSCPICLEPLREPVTLPCGHNFCLACLGALWPHRSAGGTGGSGGPARCPLCQEPFPDGLQLRKNHTLSELLQLRQGSVPGPMSAPASGSTRGATPEPSAPSAPPPAPEPSAPCAPEQWPAGEEPVRCDACPEGAALPAALSCLSCLASFCSAHLAPHERSPALRGHRLVPPLRRLEESLCPRHLRPLERYCRVERVCLCEACATQDHRGHELVPLEQERALQEVEQSKVLSAAEDRMDELGAGIAQSRRTVALIKSAAVAERERVSQMFAEATATLQSFQNEVMGFIEEGEATMLGRSQGDLRRQEEQRSRLSKARHNLGQVPEADSVSFLQELLALRLALEEGCGPGPGPPRELSFTKSSQVVKAVRDTLISACASQWEQLRGLGSNEDGLQKLGSEDVESQDPDSTSLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPESPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSVGVMAEGFSPQEPYDRGRLGRNAHSCCLQWNGRGFSVWFCGLEAPLPHAFSPTVGVCLEYADHALAFYAVRDGKLSLLRRLKASRPRRSGALASPTDPFQSRLDSHFSGLFNHRLKPAFFLESVDAHLQIGPLKKSCITVLKRR

Target ORF information:

RefSeq Version	NM_172570.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 47 (Trim47), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172570.5

ORF Insert Sequence:

ATGGACGGCA GCGGGCCTTT CAGCTGCCCC ATCTGCCTGG AACCGCTCCG GGAGCCGGTG 
ACACTACCCT GCGGCCACAA CTTCTGCCTC GCTTGCCTGG GCGCTCTCTG GCCGCACCGG 
AGCGCGGGCG GCACCGGTGG TTCCGGAGGT CCAGCTCGCT GTCCACTTTG TCAGGAGCCT 
TTCCCCGACG GCCTGCAGCT CCGCAAGAAC CACACGCTGT CCGAGTTGCT GCAGCTCCGC 
CAGGGCTCGG TCCCGGGACC CATGTCCGCC CCGGCTTCAG GATCGACCCG GGGCGCCACG 
CCCGAGCCCT CCGCGCCCAG CGCACCACCT CCGGCTCCGG AACCATCGGC TCCCTGCGCG 
CCGGAGCAGT GGCCCGCGGG TGAGGAGCCG GTGCGCTGCG ACGCGTGCCC CGAGGGCGCC 
GCCCTGCCCG CCGCGCTCTC TTGCCTCTCC TGCCTCGCCT CCTTCTGCTC CGCGCATCTG 
GCCCCCCACG AACGCAGCCC CGCCCTGCGC GGCCACCGCC TGGTGCCCCC GCTGCGCCGA 
CTTGAGGAGA GCCTGTGTCC GCGCCACCTG CGGCCTCTGG AGCGCTACTG TCGCGTGGAG 
CGTGTGTGCC TTTGCGAGGC CTGTGCCACT CAGGACCATC GCGGCCACGA GCTCGTGCCG 
CTGGAGCAGG AGCGCGCGCT TCAGGAGGTG GAGCAGTCCA AAGTCCTGAG CGCTGCGGAG 
GACCGCATGG ATGAACTGGG TGCCGGCATC GCACAGTCCC GCCGTACTGT GGCCCTCATC 
AAGAGTGCAG CTGTGGCAGA GCGGGAGAGG GTGAGCCAGA TGTTTGCCGA GGCCACAGCC 
ACCCTTCAGA GTTTCCAGAA CGAGGTGATG GGGTTCATCG AAGAGGGAGA GGCCACAATG 
TTGGGCCGGT CCCAGGGTGA CCTGCGTAGA CAAGAGGAAC AGCGCAGCCG CCTAAGCAAG 
GCACGGCACA ACCTCGGTCA GGTTCCAGAG GCGGATTCAG TCAGCTTTCT CCAGGAGCTC 
CTGGCACTAC GGCTGGCCCT GGAGGAGGGG TGCGGCCCCG GGCCTGGGCC CCCCAGGGAA 
CTCAGCTTCA CCAAGTCCTC CCAGGTGGTC AAAGCGGTGA GAGACACCCT CATCTCAGCC 
TGTGCCAGCC AGTGGGAGCA GCTGCGGGGG CTGGGCAGCA ATGAGGATGG GCTACAGAAA 
CTCGGCTCAG AAGATGTGGA GTCCCAGGAC CCTGACAGTA CCAGCCTGCT GGAGAGTGAG 
GCCCCCAGGG ATTACTTCCT CAAGTTTGCC TACATCGTGG ACCTGGACAG CGACACCGCG 
GACAAGTTCC TGCAGCTTTT TGGAACTAAA GGTGTCAAGA GAGTACTGTG TCCCATCAAC 
TACCCGGAGT CACCCACCCG CTTCACACAC TGCGAGCAGG TGCTAGGAGA GGGCGCCCTG 
GACCGGGGCA CCTACTACTG GGAGGTGGAG ATCATCGAAG GATGGGTCAG TGTGGGAGTC 
ATGGCCGAGG GCTTCTCCCC GCAAGAGCCC TATGACCGGG GCCGGCTGGG CCGCAATGCA 
CACTCATGCT GTCTGCAGTG GAATGGGCGT GGCTTCTCAG TCTGGTTCTG CGGGCTGGAG 
GCCCCGCTAC CCCATGCCTT TTCGCCTACA GTCGGGGTCT GCCTAGAGTA TGCAGACCAT 
GCCCTGGCCT TCTATGCTGT CCGAGACGGA AAGCTGAGCC TTCTTCGGAG GCTCAAAGCC 
TCCCGGCCTC GCCGCAGCGG TGCTCTGGCC TCCCCCACTG ACCCTTTTCA GAGTCGCCTG 
GACAGCCATT TTTCCGGGCT CTTCAACCAC AGGCTCAAGC CTGCCTTCTT CCTGGAGAGT 
GTGGACGCCC ATCTGCAGAT TGGACCTCTC AAGAAATCGT GCATAACCGT GCTGAAGAGG 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The deubiquitinating enzyme cylindromatosis mitigates nonalcoholic steatohepatitis.
Nature medicine(2018 Jan)
Ji YX,Huang Z,Yang X,Wang X,Zhao LP,Wang PX,Zhang XJ,Alves-Bezerra M,Cai L,Zhang P,Lu YX,Bai L,Gao MM,Zhao H,Tian S,Wang Y,Huang ZX,Zhu XY,Zhang Y,Gong J,She ZG,Li F,Cohen DE,Li H

Expression of LIM-homeodomain transcription factors in the developing and mature mouse retina.
Gene expression patterns : GEP14(1)1-8(2014 Jan)
Balasubramanian R,Bui A,Ding Q,Gan L

Elucidation of the transcription network governing mammalian sex determination by exploiting strain-specific susceptibility to sex reversal.
Genes & development23(21)2521-36(2009 Nov)
Munger SC,Aylor DL,Syed HA,Magwene PM,Threadgill DW,Capel B

A multipotent progenitor domain guides pancreatic organogenesis.
Developmental cell13(1)103-14(2007 Jul)
Zhou Q,Law AC,Rajagopal J,Anderson WJ,Gray PA,Melton DA