Ptrh2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptrh2
Entrez Gene ID	217057
Full Name	peptidyl-tRNA hydrolase 2
Synonyms	A230072I16Rik,Bit1,CGI-147
General protein information	Preferred Names peptidyl-tRNA hydrolase 2 Names peptidyl-tRNA hydrolase 2, mitochondrial PTH 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_175004.2	NP_778169.1	peptidyl-tRNA hydrolase 2, mitochondrial isoform a
NM_175004.3	NP_778169.1	peptidyl-tRNA hydrolase 2, mitochondrial isoform a
NM_001098810.2	NP_001092280.1	peptidyl-tRNA hydrolase 2, mitochondrial isoform b
NM_001098810.3	NP_001092280.1	peptidyl-tRNA hydrolase 2, mitochondrial isoform b

Pathways from BioSystems

Homologies

	PTRH2	XP_001109316.2
Rattus norvegicus (Norway rat)	Ptrh2	NP_001013882.1
Arabidopsis thaliana (thale cress)	AT3G03010	NP_001030627.1
Pan troglodytes (chimpanzee)	PTRH2	XP_003953200.1
Gallus gallus (chicken)	PTRH2	NP_001035503.1
Canis lupus familiaris (dog)	PTRH2	XP_003435333.1
Danio rerio (zebrafish)	ptrh2	NP_001018371.2
Magnaporthe oryzae (rice blast fungus)	MGG_01099	XP_003717835.1
Neurospora crassa	NCU01501	XP_956950.2
Homo sapiens (human)	PTRH2	NP_057161.1
Drosophila melanogaster (fruit fly)	CG1307	NP_649675.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003883	XP_308109.5
Xenopus tropicalis (tropical clawed frog)	ptrh2	NP_001016657.1
Bos taurus (cattle)	PTRH2	NP_001029691.1
Mus musculus (house mouse)	Ptrh2	NP_778169.1
Arabidopsis thaliana (thale cress)	AT5G16870	NP_568340.1
Oryza sativa (rice)	Os04g0652900	NP_001054102.1

Gene Ontology
Bibliography

Related articles in PubMed

PTRH2 gene mutation causes progressive congenital skeletal muscle pathology.
Doe J, Kaindl AM, Jijiwa M, de la Vega M, Hu H, Griffiths GS, Fontelonga TM, Barraza P, Cruz V, Van Ry P, Ramos JW, Burkin DJ, Matter ML
Human molecular genetics26(8)1458-1464(2017 04)

Bit-1 is an essential regulator of myogenic differentiation.
Griffiths GS, Doe J, Jijiwa M, Van Ry P, Cruz V, de la Vega M, Ramos JW, Burkin DJ, Matter ML
Journal of cell science128(9)1707-17(2015 May)

Mutations in PTRH2 cause novel infantile-onset multisystem disease with intellectual disability, microcephaly, progressive ataxia, and muscle weakness.
Hu H, Matter ML, Issa-Jahns L, Jijiwa M, Kraemer N, Musante L, de la Vega M, Ninnemann O, Schindler D, Damatova N, Eirich K, Sifringer M, Schr?tter S, Eickholt BJ, van den Heuvel L, Casamina C, Stoltenburg-Didinger G, Ropers HH, Wienker TF, H?bner C, Kaindl AM
Annals of clinical and translational neurology1(12)1024-35(2014 Dec)

Bit-1 mediates integrin-dependent cell survival through activation of the NFkappaB pathway.
Griffiths GS, Grundl M, Leychenko A, Reiter S, Young-Robbins SS, Sulzmaier FJ, Caliva MJ, Ramos JW, Matter ML
The Journal of biological chemistry286(16)14713-23(2011 Apr)

Metastasis of tumor cells is enhanced by downregulation of Bit1.
Karmali PP, Brunquell C, Tram H, Ireland SK, Ruoslahti E, Biliran H
PloS one6(8)e23840(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ptrh2 levels were up-regulated in dystrophin deficient mdx muscle, which correlates with the elevated levels of the a7b1 integrin observed in mdx muscle and Duchenne muscular dystrophy patients. Similar to the a7 integrin, Ptrh2 expression was decreased in laminin-a2 null gastrocnemius muscle
Title: PTRH2 gene mutation causes progressive congenital skeletal muscle pathology.

These results support an unanticipated yet essential role for Bit-1 in controlling myogenesis through regulation of Bcl-2.
Title: Bit-1 is an essential regulator of myogenic differentiation.

Bit-1 mediates integrin-dependent cell survival through activation of the NFkappaB pathway
Title: Bit-1 mediates integrin-dependent cell survival through activation of the NFkappaB pathway.

downregulation of Bit1 specifically potentiated tumor metastasis in vivo
Title: Metastasis of tumor cells is enhanced by downregulation of Bit1.

These studies establish the physiological significance of Bit1 activity and begin to delineate a Bit1 signaling pathway that acts through Erk regulation.
Title: Anoikis effector Bit1 negatively regulates Erk activity.

The following Ptrh2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptrh2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12994	NM_001098810.3 Latest version!	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13599	NM_175004.3 Latest version!	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu13599	NM_175004.2	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu12994	NM_001098810.2	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptrh2 ( NM_001098810.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001092280.1 Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12994
Clone ID Related Accession (Same CDS sequence)	NM_001098810.2 , NM_001098810.3
Accession Version	NM_001098810.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Mus musculus(house mouse)
Product	peptidyl-tRNA hydrolase 2, mitochondrial isoform b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK031620.1. On Jul 4, 2007 this sequence version replaced NM_001098810.1. ##Evidence-Data-START## Transcript exon combination :: AK031620.1, AK038888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCTCTCCA AGTTCTTGAC TATGGAGTAT TTGGTTCATC CTGGTACACT CAGCTTGGCT 
GCTGGAGTTG CTTGTGGCAT GTGCCTGGGC TGGGGCCTCC GGAGCCACCT TGGGATGTTC 
CCCCAGAACT CAACAAGTGA GGCAAATAGA GACACAGAGA CTGGAACTGA AGCAAGCATC 
TTGGGAGAGA GTGGGGAATA CAAAATGATT CTTGTGGTTC GAACTGACCT TAAAATGGGG 
AAAGGGAAGG TTGCTGCCCA GTGTTCTCAT GCTGCCGTGT CTGCCTACAA GCAGACTCAA 
AGGAGAAGCC CTCAAGTGCT CAAAGAGTGG GAGTACTGTG GCCAGCCCAA AGTGGTGGTC 
AAAGCTCCAG ATGAAGACAC CCTCATTCAA TTACTCACCC ATGCAAAAAC CTTGGGACTG 
ACTGTAAGTT TAATCCAAGA TGCTGGACGT ACTCAGATTG AACCAGGTTC TCGAACTGTC 
CTGGGAATTG GGCCAGGACC AGTAGAACTA ATTGATGAAG TTACTGGCCA CCTAAAACTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092280.1
CDS	44..589
Translation	MLSKFLTMEYLVHPGTLSLAAGVACGMCLGWGLRSHLGMFPQNSTSEANRDTETGTEASILGESGEYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDEDTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKLY

Target ORF information:

RefSeq Version	NM_001098810.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098810.2

ORF Insert Sequence:

ATGCTCTCCA AGTTCTTGAC TATGGAGTAT TTGGTTCATC CTGGTACACT CAGCTTGGCT 
GCTGGAGTTG CTTGTGGCAT GTGCCTGGGC TGGGGCCTCC GGAGCCACCT TGGGATGTTC 
CCCCAGAACT CAACAAGTGA GGCAAATAGA GACACAGAGA CTGGAACTGA AGCAAGCATC 
TTGGGAGAGA GTGGGGAATA CAAAATGATT CTTGTGGTTC GAACTGACCT TAAAATGGGG 
AAAGGGAAGG TTGCTGCCCA GTGTTCTCAT GCTGCCGTGT CTGCCTACAA GCAGACTCAA 
AGGAGAAGCC CTCAAGTGCT CAAAGAGTGG GAGTACTGTG GCCAGCCCAA AGTGGTGGTC 
AAAGCTCCAG ATGAAGACAC CCTCATTCAA TTACTCACCC ATGCAAAAAC CTTGGGACTG 
ACTGTAAGTT TAATCCAAGA TGCTGGACGT ACTCAGATTG AACCAGGTTC TCGAACTGTC 
CTGGGAATTG GGCCAGGACC AGTAGAACTA ATTGATGAAG TTACTGGCCA CCTAAAACTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptrh2 ( NM_001098810.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001092280.1 Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12994
Clone ID Related Accession (Same CDS sequence)	NM_001098810.2 , NM_001098810.3
Accession Version	NM_001098810.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	peptidyl-tRNA hydrolase 2, mitochondrial isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA271949.1, AK031620.1 and AK038888.1. On Jul 6, 2018 this sequence version replaced NM_001098810.2. ##Evidence-Data-START## Transcript exon combination :: AK031620.1, AK038888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCTCCA AGTTCTTGAC TATGGAGTAT TTGGTTCATC CTGGTACACT CAGCTTGGCT 
GCTGGAGTTG CTTGTGGCAT GTGCCTGGGC TGGGGCCTCC GGAGCCACCT TGGGATGTTC 
CCCCAGAACT CAACAAGTGA GGCAAATAGA GACACAGAGA CTGGAACTGA AGCAAGCATC 
TTGGGAGAGA GTGGGGAATA CAAAATGATT CTTGTGGTTC GAACTGACCT TAAAATGGGG 
AAAGGGAAGG TTGCTGCCCA GTGTTCTCAT GCTGCCGTGT CTGCCTACAA GCAGACTCAA 
AGGAGAAGCC CTCAAGTGCT CAAAGAGTGG GAGTACTGTG GCCAGCCCAA AGTGGTGGTC 
AAAGCTCCAG ATGAAGACAC CCTCATTCAA TTACTCACCC ATGCAAAAAC CTTGGGACTG 
ACTGTAAGTT TAATCCAAGA TGCTGGACGT ACTCAGATTG AACCAGGTTC TCGAACTGTC 
CTGGGAATTG GGCCAGGACC AGTAGAACTA ATTGATGAAG TTACTGGCCA CCTAAAACTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092280.1
CDS	51..596
Translation	MLSKFLTMEYLVHPGTLSLAAGVACGMCLGWGLRSHLGMFPQNSTSEANRDTETGTEASILGESGEYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDEDTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKLY

Target ORF information:

RefSeq Version	NM_001098810.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098810.3

ORF Insert Sequence:

ATGCTCTCCA AGTTCTTGAC TATGGAGTAT TTGGTTCATC CTGGTACACT CAGCTTGGCT 
GCTGGAGTTG CTTGTGGCAT GTGCCTGGGC TGGGGCCTCC GGAGCCACCT TGGGATGTTC 
CCCCAGAACT CAACAAGTGA GGCAAATAGA GACACAGAGA CTGGAACTGA AGCAAGCATC 
TTGGGAGAGA GTGGGGAATA CAAAATGATT CTTGTGGTTC GAACTGACCT TAAAATGGGG 
AAAGGGAAGG TTGCTGCCCA GTGTTCTCAT GCTGCCGTGT CTGCCTACAA GCAGACTCAA 
AGGAGAAGCC CTCAAGTGCT CAAAGAGTGG GAGTACTGTG GCCAGCCCAA AGTGGTGGTC 
AAAGCTCCAG ATGAAGACAC CCTCATTCAA TTACTCACCC ATGCAAAAAC CTTGGGACTG 
ACTGTAAGTT TAATCCAAGA TGCTGGACGT ACTCAGATTG AACCAGGTTC TCGAACTGTC 
CTGGGAATTG GGCCAGGACC AGTAGAACTA ATTGATGAAG TTACTGGCCA CCTAAAACTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptrh2 ( NM_175004.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_778169.1 Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13599
Clone ID Related Accession (Same CDS sequence)	NM_175004.2 , NM_175004.3
Accession Version	NM_175004.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Mus musculus(house mouse)
Product	peptidyl-tRNA hydrolase 2, mitochondrial isoform a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL592222.11. On Nov 17, 2006 this sequence version replaced NM_175004.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK028555.1, AK086996.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGATGCTCT CCAAGTTCTT GACTATGGAG TATTTGGTTC ATCCTGGTAC ACTCAGCTTG 
GCTGCTGGAG TTGCTTGTGG CATGTGCCTG GGCTGGGGCC TCCGGAGCCA CCTTGGGATG 
TTCCCCCAGA ACTCAACAAG TGAGGCAAAT AGAGACACAG AGACTGGAAC TGAAGCAAGC 
ATCTTGGGAG AGAGTGGGGA ATACAAAATG ATTCTTGTGG TTCGAACTGA CCTTAAAATG 
GGGAAAGGGA AGGTTGCTGC CCAGTGTTCT CATGCTGCCG TGTCTGCCTA CAAGCAGACT 
CAAAGGAGAA GCCCTCAAGT GCTCAAAGAG TGGGAGTACT GTGGCCAGCC CAAAGTGGTG 
GTCAAAGCTC CAGATGAAGA CACCCTCATT CAATTACTCA CCCATGCAAA AACCTTGGGA 
CTGACTGTAA GTTTAATCCA AGATGCTGGA CGTACTCAGA TTGAACCAGG TTCTCGAACT 
GTCCTGGGAA TTGGGCCAGG ACCAGTAGAA CTAATTGATG AAGTTACTGG CCACCTAAAA 
CTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778169.1
CDS	232..780
Translation	MMLSKFLTMEYLVHPGTLSLAAGVACGMCLGWGLRSHLGMFPQNSTSEANRDTETGTEASILGESGEYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDEDTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKLY

Target ORF information:

RefSeq Version	NM_175004.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175004.2

ORF Insert Sequence:

ATGATGCTCT CCAAGTTCTT GACTATGGAG TATTTGGTTC ATCCTGGTAC ACTCAGCTTG 
GCTGCTGGAG TTGCTTGTGG CATGTGCCTG GGCTGGGGCC TCCGGAGCCA CCTTGGGATG 
TTCCCCCAGA ACTCAACAAG TGAGGCAAAT AGAGACACAG AGACTGGAAC TGAAGCAAGC 
ATCTTGGGAG AGAGTGGGGA ATACAAAATG ATTCTTGTGG TTCGAACTGA CCTTAAAATG 
GGGAAAGGGA AGGTTGCTGC CCAGTGTTCT CATGCTGCCG TGTCTGCCTA CAAGCAGACT 
CAAAGGAGAA GCCCTCAAGT GCTCAAAGAG TGGGAGTACT GTGGCCAGCC CAAAGTGGTG 
GTCAAAGCTC CAGATGAAGA CACCCTCATT CAATTACTCA CCCATGCAAA AACCTTGGGA 
CTGACTGTAA GTTTAATCCA AGATGCTGGA CGTACTCAGA TTGAACCAGG TTCTCGAACT 
GTCCTGGGAA TTGGGCCAGG ACCAGTAGAA CTAATTGATG AAGTTACTGG CCACCTAAAA 
CTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptrh2 ( NM_175004.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_778169.1 Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13599
Clone ID Related Accession (Same CDS sequence)	NM_175004.2 , NM_175004.3
Accession Version	NM_175004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Mus musculus(house mouse)
Product	peptidyl-tRNA hydrolase 2, mitochondrial isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592222.11. On Jul 7, 2018 this sequence version replaced NM_175004.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK086996.1, AK028555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGCTCT CCAAGTTCTT GACTATGGAG TATTTGGTTC ATCCTGGTAC ACTCAGCTTG 
GCTGCTGGAG TTGCTTGTGG CATGTGCCTG GGCTGGGGCC TCCGGAGCCA CCTTGGGATG 
TTCCCCCAGA ACTCAACAAG TGAGGCAAAT AGAGACACAG AGACTGGAAC TGAAGCAAGC 
ATCTTGGGAG AGAGTGGGGA ATACAAAATG ATTCTTGTGG TTCGAACTGA CCTTAAAATG 
GGGAAAGGGA AGGTTGCTGC CCAGTGTTCT CATGCTGCCG TGTCTGCCTA CAAGCAGACT 
CAAAGGAGAA GCCCTCAAGT GCTCAAAGAG TGGGAGTACT GTGGCCAGCC CAAAGTGGTG 
GTCAAAGCTC CAGATGAAGA CACCCTCATT CAATTACTCA CCCATGCAAA AACCTTGGGA 
CTGACTGTAA GTTTAATCCA AGATGCTGGA CGTACTCAGA TTGAACCAGG TTCTCGAACT 
GTCCTGGGAA TTGGGCCAGG ACCAGTAGAA CTAATTGATG AAGTTACTGG CCACCTAAAA 
CTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778169.1
CDS	141..689
Translation	MMLSKFLTMEYLVHPGTLSLAAGVACGMCLGWGLRSHLGMFPQNSTSEANRDTETGTEASILGESGEYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDEDTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKLY

Target ORF information:

RefSeq Version	NM_175004.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus peptidyl-tRNA hydrolase 2 (Ptrh2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175004.3

ORF Insert Sequence:

ATGATGCTCT CCAAGTTCTT GACTATGGAG TATTTGGTTC ATCCTGGTAC ACTCAGCTTG 
GCTGCTGGAG TTGCTTGTGG CATGTGCCTG GGCTGGGGCC TCCGGAGCCA CCTTGGGATG 
TTCCCCCAGA ACTCAACAAG TGAGGCAAAT AGAGACACAG AGACTGGAAC TGAAGCAAGC 
ATCTTGGGAG AGAGTGGGGA ATACAAAATG ATTCTTGTGG TTCGAACTGA CCTTAAAATG 
GGGAAAGGGA AGGTTGCTGC CCAGTGTTCT CATGCTGCCG TGTCTGCCTA CAAGCAGACT 
CAAAGGAGAA GCCCTCAAGT GCTCAAAGAG TGGGAGTACT GTGGCCAGCC CAAAGTGGTG 
GTCAAAGCTC CAGATGAAGA CACCCTCATT CAATTACTCA CCCATGCAAA AACCTTGGGA 
CTGACTGTAA GTTTAATCCA AGATGCTGGA CGTACTCAGA TTGAACCAGG TTCTCGAACT 
GTCCTGGGAA TTGGGCCAGG ACCAGTAGAA CTAATTGATG AAGTTACTGG CCACCTAAAA 
CTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PTRH2 gene mutation causes progressive congenital skeletal muscle pathology.
Human molecular genetics26(8)1458-1464(2017 04)
Doe J,Kaindl AM,Jijiwa M,de la Vega M,Hu H,Griffiths GS,Fontelonga TM,Barraza P,Cruz V,Van Ry P,Ramos JW,Burkin DJ,Matter ML

Bit-1 is an essential regulator of myogenic differentiation.
Journal of cell science128(9)1707-17(2015 May)
Griffiths GS,Doe J,Jijiwa M,Van Ry P,Cruz V,de la Vega M,Ramos JW,Burkin DJ,Matter ML

Mutations in PTRH2 cause novel infantile-onset multisystem disease with intellectual disability, microcephaly, progressive ataxia, and muscle weakness.
Annals of clinical and translational neurology1(12)1024-35(2014 Dec)
Hu H,Matter ML,Issa-Jahns L,Jijiwa M,Kraemer N,Musante L,de la Vega M,Ninnemann O,Schindler D,Damatova N,Eirich K,Sifringer M,Schr?tter S,Eickholt BJ,van den Heuvel L,Casamina C,Stoltenburg-Didinger G,Ropers HH,Wienker TF,H?bner C,Kaindl AM

Bit-1 mediates integrin-dependent cell survival through activation of the NFkappaB pathway.
The Journal of biological chemistry286(16)14713-23(2011 Apr)
Griffiths GS,Grundl M,Leychenko A,Reiter S,Young-Robbins SS,Sulzmaier FJ,Caliva MJ,Ramos JW,Matter ML

Metastasis of tumor cells is enhanced by downregulation of Bit1.
PloS one6(8)e23840(2011)
Karmali PP,Brunquell C,Tram H,Ireland SK,Ruoslahti E,Biliran H

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Ptrh2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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