Dynlt1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dynlt1b
Entrez Gene ID	21648
Full Name	dynein light chain Tctex-type 1B
Synonyms	AGS2,Dynlt1,Tctex-1,Tctex1
General protein information	Preferred Names dynein light chain Tctex-type 1B Names dynein light chain Tctex-type 1 T-complex testis-specific protein 1 activator of G-protein signaling 2 dynein light chain Tctex-type 1D t-complex testis expressed 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A1\|17 3.97 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009342.2	NP_033368.1	dynein light chain Tctex-type 1
XM_030249643.1	XP_030105503.1	dynein light chain Tctex-type 1 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Dlc90F	NP_477356.1
Homo sapiens (human)	DYNLT1	NP_006510.1
Bos taurus (cattle)	DYNLT1	NP_777045.1
Mus musculus (house mouse)	Dynlt1f	NP_001160099.1
Mus musculus (house mouse)	Dynlt1a	NP_001160101.1
Danio rerio (zebrafish)	dynlt1	NP_001129255.1
Pan troglodytes (chimpanzee)	DYNLT1	XP_001147088.1
Gallus gallus (chicken)	DYNLT1	XP_001232357.1
Mus musculus (house mouse)	Dynlt1c	NP_001160102.1
Xenopus tropicalis (tropical clawed frog)	dynlt1	NP_001011428.1
Canis lupus familiaris (dog)	DYNLT1	XP_003638830.1
Mus musculus (house mouse)	Dynlt1b	NP_033368.1
Rattus norvegicus (Norway rat)	Dynlt1	NP_112608.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001229	XP_321929.3

Gene Ontology
Bibliography

Related articles in PubMed

MAP6 interacts with Tctex1 and Ca
Brocard J, Dufour F, Gory-Faur? S, Arnoult C, Bosc C, Denarier E, Peris L, Saoudi Y, De Waard M, Andrieux A
The European journal of neuroscience46(11)2754-2767(2017 Dec)

Molecular Basis for the Protein Recognition Specificity of the Dynein Light Chain DYNLT1/Tctex1: CHARACTERIZATION OF THE INTERACTION WITH ACTIVIN RECEPTOR IIB.
Merino-Gracia J, Zamora-Carreras H, Bruix M, Rodr?guez-Crespo I
The Journal of biological chemistry291(40)20962-20975(2016 Sep)

Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption.
Pavlos NJ, Cheng TS, Qin A, Ng PY, Feng HT, Ang ES, Carrello A, Sung CH, Jahn R, Zheng MH, Xu J
Molecular and cellular biology31(7)1551-64(2011 Apr)

Dynein light chain Tctex-type 1 modulates orexin signaling through its interaction with orexin 1 receptor.
Duguay D, B?langer-Nelson E, Mongrain V, Beben A, Khatchadourian A, Cermakian N
PloS one6(10)e26430(2011)

Identification of the Tctex-1 regulatory element that directs expression to neural stem/progenitor cells in developing and adult brain.
Tseng YY, Gruzdeva N, Li A, Chuang JZ, Sung CH
The Journal of comparative neurology518(16)3327-42(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

activin receptor IIB as a novel DYNLT1 ligand and suggest that DYNLT1 functions as a molecular dimerization engine bringing together two receptor monomers in the cytoplasmic side of the membrane.
Title: Molecular Basis for the Protein Recognition Specificity of the Dynein Light Chain DYNLT1/Tctex1: CHARACTERIZATION OF THE INTERACTION WITH ACTIVIN RECEPTOR IIB.

Targeted disruption of Tctex-1 by RNA interference significantly impairs bone resorption capacity and mislocalizes Rab3D vesicles in osteoclasts.
Title: Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption.

Dynlt1 modulates orexin signaling by regulating OX1R
Title: Dynein light chain Tctex-type 1 modulates orexin signaling through its interaction with orexin 1 receptor.

Results identify a regulatory region of the Tctex-1 gene that is capable of directing transgenic expression of green fluorescent protein (GFP) reporter that recapitulates the spatial and temporal expression pattern of endogenous Tctex-1.
Title: Identification of the Tctex-1 regulatory element that directs expression to neural stem/progenitor cells in developing and adult brain.

Thus, Lfc and Tctex-1 interact to regulate cortical neurogenesis, potentially by regulating mitotic spindle orientation.
Title: Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells.

The following Dynlt1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dynlt1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03972	NM_009342.2 Latest version!	Mus musculus dynein light chain Tctex-type 1B (Dynlt1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu03972	XM_030249643.1 Latest version!	Mus musculus dynein light chain Tctex-type 1B (Dynlt1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dynlt1b ( NM_009342.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033368.1 Mus musculus dynein light chain Tctex-type 1B (Dynlt1b), mRNA.

Price & Availability↑

CloneID	OMu03972
Clone ID Related Accession (Same CDS sequence)	NM_009342.2 , XM_030249643.1
Accession Version	NM_009342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	dynein light chain Tctex-type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC043018.1. On or before May 4, 2006 this sequence version replaced XM_001006801.1, NM_009342.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043018.1, CB572221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGACT TCCAGGCCTC AGAGGAGACT GCATTTGTTG TTGATGAAGT GAGCAGCATT 
GTAAAGGAGG CTATAGAAAG CGCCATCGGT GGTAATGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTCCT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAGACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAACG GTGCTGGGTT ACACTCCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACAGAC GGAAGCTGCA CAGTCCGATG GGAGAACAAG 
ACCATGTACT GCATCGTCAG TACCTTCGGA CTGTCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033368.1
CDS	101..442
Translation	MEDFQASEETAFVVDEVSSIVKEAIESAIGGNAYQHSKVNQWTTNVLEQTLSQLTKLGRPFKYIVTCVIMQKNGAGLHSASSCFWDSSTDGSCTVRWENKTMYCIVSTFGLSI

Target ORF information:

RefSeq Version	NM_009342.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein light chain Tctex-type 1B (Dynlt1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009342.2

ORF Insert Sequence:

ATGGAAGACT TCCAGGCCTC AGAGGAGACT GCATTTGTTG TTGATGAAGT GAGCAGCATT 
GTAAAGGAGG CTATAGAAAG CGCCATCGGT GGTAATGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTCCT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAGACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAACG GTGCTGGGTT ACACTCCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACAGAC GGAAGCTGCA CAGTCCGATG GGAGAACAAG 
ACCATGTACT GCATCGTCAG TACCTTCGGA CTGTCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dynlt1b ( XM_030249643.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105503.1 Mus musculus dynein light chain Tctex-type 1B (Dynlt1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03972
Clone ID Related Accession (Same CDS sequence)	NM_009342.2 , XM_030249643.1
Accession Version	XM_030249643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dynein light chain Tctex-type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACT TCCAGGCCTC AGAGGAGACT GCATTTGTTG TTGATGAAGT GAGCAGCATT 
GTAAAGGAGG CTATAGAAAG CGCCATCGGT GGTAATGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTCCT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAGACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAACG GTGCTGGGTT ACACTCCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACAGAC GGAAGCTGCA CAGTCCGATG GGAGAACAAG 
ACCATGTACT GCATCGTCAG TACCTTCGGA CTGTCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105503.1
CDS	683..1024
Translation	MEDFQASEETAFVVDEVSSIVKEAIESAIGGNAYQHSKVNQWTTNVLEQTLSQLTKLGRPFKYIVTCVIMQKNGAGLHSASSCFWDSSTDGSCTVRWENKTMYCIVSTFGLSI

Target ORF information:

RefSeq Version	XM_030249643.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein light chain Tctex-type 1B (Dynlt1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249643.1

ORF Insert Sequence:

ATGGAAGACT TCCAGGCCTC AGAGGAGACT GCATTTGTTG TTGATGAAGT GAGCAGCATT 
GTAAAGGAGG CTATAGAAAG CGCCATCGGT GGTAATGCCT ACCAGCACAG CAAAGTCAAC 
CAGTGGACCA CTAATGTCCT AGAACAGACT TTGAGCCAAC TCACCAAACT GGGGAGACCA 
TTTAAATACA TTGTGACCTG TGTGATCATG CAGAAGAACG GTGCTGGGTT ACACTCCGCA 
AGTTCCTGCT TCTGGGACAG CTCCACAGAC GGAAGCTGCA CAGTCCGATG GGAGAACAAG 
ACCATGTACT GCATCGTCAG TACCTTCGGA CTGTCCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MAP6 interacts with Tctex1 and Ca
The European journal of neuroscience46(11)2754-2767(2017 Dec)
Brocard J,Dufour F,Gory-Faur? S,Arnoult C,Bosc C,Denarier E,Peris L,Saoudi Y,De Waard M,Andrieux A

Molecular Basis for the Protein Recognition Specificity of the Dynein Light Chain DYNLT1/Tctex1: CHARACTERIZATION OF THE INTERACTION WITH ACTIVIN RECEPTOR IIB.
The Journal of biological chemistry291(40)20962-20975(2016 Sep)
Merino-Gracia J,Zamora-Carreras H,Bruix M,Rodr?guez-Crespo I

Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption.
Molecular and cellular biology31(7)1551-64(2011 Apr)
Pavlos NJ,Cheng TS,Qin A,Ng PY,Feng HT,Ang ES,Carrello A,Sung CH,Jahn R,Zheng MH,Xu J

Dynein light chain Tctex-type 1 modulates orexin signaling through its interaction with orexin 1 receptor.
PloS one6(10)e26430(2011)
Duguay D,B?langer-Nelson E,Mongrain V,Beben A,Khatchadourian A,Cermakian N

Identification of the Tctex-1 regulatory element that directs expression to neural stem/progenitor cells in developing and adult brain.
The Journal of comparative neurology518(16)3327-42(2010 Aug)
Tseng YY,Gruzdeva N,Li A,Chuang JZ,Sung CH