Klhl25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klhl25
Entrez Gene ID	207952
Full Name	kelch-like 25
Synonyms	2810402K13Rik,BC027373,Enc-1
General protein information	Preferred Names kelch-like 25 Names kelch-like protein 25 ENC-2 ectoderm-neural cortex protein 2 ectoderm-neural cortex-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122780.1	NP_001116252.1	kelch-like protein 25
NM_182782.2	NP_877583.1	kelch-like protein 25
XM_011250832.2	XP_011249134.1	kelch-like protein 25 isoform X1
XM_011250832.3	XP_011249134.1	kelch-like protein 25 isoform X1
NM_029652.1	NP_083928.1	kelch-like protein 25

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KLHL25	NP_001179615.1
	KLHL25	XP_001086777.1
Homo sapiens (human)	KLHL25	NP_071925.2
Canis lupus familiaris (dog)	KLHL25	XP_005618396.1
Pan troglodytes (chimpanzee)	KLHL25	XP_510571.2
Rattus norvegicus (Norway rat)	Klhl25	NP_001034095.1
Gallus gallus (chicken)	KLHL25	XP_004943913.1
Mus musculus (house mouse)	Klhl25	NP_001116252.1
Xenopus tropicalis (tropical clawed frog)	klhl25	NP_001072541.1

Gene Ontology
Bibliography

Related articles in PubMed

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
Nishimura M, Yokoi N, Miki T, Horikawa Y, Yoshioka H, Takeda J, Ohara O, Seino S
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)

Genomic analysis of mouse retinal development.
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL
PLoS biology2(9)E247(2004 Sep)

Genome-wide, large-scale production of mutant mice by ENU mutagenesis.
Hrab? de Angelis MH, Flaswinkel H, Fuchs H, Rathkolb B, Soewarto D, Marschall S, Heffner S, Pargent W, Wuensch K, Jung M, Reis A, Richter T, Alessandrini F, Jakob T, Fuchs E, Kolb H, Kremmer E, Schaeble K, Rollinski B, Roscher A, Peters C, Meitinger T, Strom T, Steckler T, Holsboer F, Klopstock T, Gekeler F, Schindewolf C, Jung T, Avraham K, Behrendt H, Ring J, Zimmer A, Schughart K, Pfeffer K, Wolf E, Balling R
Nature genetics25(4)444-7(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Klhl25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klhl25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05747	NM_029652.1 Latest version!	Mus musculus kelch-like 25 (Klhl25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05747	NM_001122780.1 Latest version!	Mus musculus kelch-like 25 (Klhl25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05747	NM_182782.2 Latest version!	Mus musculus kelch-like 25 (Klhl25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05747	XM_011250832.3 Latest version!	Mus musculus kelch-like 25 (Klhl25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu05747	XM_011250832.2	Mus musculus kelch-like 25 (Klhl25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klhl25 ( NM_001122780.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001116252.1 Mus musculus kelch-like 25 (Klhl25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05747
Clone ID Related Accession (Same CDS sequence)	NM_001122780.1 , NM_182782.2 , XM_011250832.2 , XM_011250832.3 , NM_029652.1
Accession Version	NM_001122780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	kelch-like protein 25
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY241272.1 and AK143086.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same the protein. ##Evidence-Data-START## Transcript exon combination :: AK143086.1, BC011221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116252.1
CDS	148..1917
Translation	MSVSVHETRKSRSSTGSMNISVFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIVINEENAESLLEAGDMLQFHDVRDAAAEFLEKNLSPSNCLGMMVLSDAHQCRRLYEFSCRMSLVHFETVRQSEDFNSLSRDTLLDLISRDELETEDERVVFEAILQWVKHDLEQRKAHLPLLLRNVRLALLPSDCLKNAVSGEALLMADECTKLILDEAFRCKTKILLNDGVVTSPFARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGIFPASPSVSLKQVEKYDPGDNKWTMVAPMRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCFDPSENRWTIKAECPQPWRYTAAAVLGSQIFIMGGDTEYTAASAYRFDCETNQWTRIGDMTAKRMSCHAVASGNKLYVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA

Target ORF information:

RefSeq Version	NM_001122780.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kelch-like 25 (Klhl25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122780.1

ORF Insert Sequence:

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl25 ( NM_182782.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_877583.1 Mus musculus kelch-like 25 (Klhl25), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu05747
Clone ID Related Accession (Same CDS sequence)	NM_001122780.1 , NM_182782.2 , XM_011250832.2 , XM_011250832.3 , NM_029652.1
Accession Version	NM_182782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	kelch-like protein 25
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY241272.1, BY042513.1, AK143086.1 and BP767188.1. On Mar 22, 2008 this sequence version replaced NM_182782.1. Transcript Variant: This variant (3) differs in the 3' UTR, compared to variant 1. Variants 1-3 encode the same the protein. ##Evidence-Data-START## Transcript exon combination :: BC027373.1, BG923510.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_877583.1
CDS	159..1928
Translation	MSVSVHETRKSRSSTGSMNISVFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIVINEENAESLLEAGDMLQFHDVRDAAAEFLEKNLSPSNCLGMMVLSDAHQCRRLYEFSCRMSLVHFETVRQSEDFNSLSRDTLLDLISRDELETEDERVVFEAILQWVKHDLEQRKAHLPLLLRNVRLALLPSDCLKNAVSGEALLMADECTKLILDEAFRCKTKILLNDGVVTSPFARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGIFPASPSVSLKQVEKYDPGDNKWTMVAPMRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCFDPSENRWTIKAECPQPWRYTAAAVLGSQIFIMGGDTEYTAASAYRFDCETNQWTRIGDMTAKRMSCHAVASGNKLYVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA

Target ORF information:

RefSeq Version	NM_182782.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kelch-like 25 (Klhl25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182782.2

ORF Insert Sequence:

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl25 ( NM_029652.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083928.1 Mus musculus kelch-like 25 (Klhl25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05747
Clone ID Related Accession (Same CDS sequence)	NM_001122780.1 , NM_182782.2 , XM_011250832.2 , XM_011250832.3 , NM_029652.1
Accession Version	NM_029652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	kelch-like protein 25
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY241272.1, BY042513.1 and AK143086.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 encode the same the protein. ##Evidence-Data-START## Transcript exon combination :: BC022930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083928.1
CDS	159..1928
Translation	MSVSVHETRKSRSSTGSMNISVFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIVINEENAESLLEAGDMLQFHDVRDAAAEFLEKNLSPSNCLGMMVLSDAHQCRRLYEFSCRMSLVHFETVRQSEDFNSLSRDTLLDLISRDELETEDERVVFEAILQWVKHDLEQRKAHLPLLLRNVRLALLPSDCLKNAVSGEALLMADECTKLILDEAFRCKTKILLNDGVVTSPFARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGIFPASPSVSLKQVEKYDPGDNKWTMVAPMRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCFDPSENRWTIKAECPQPWRYTAAAVLGSQIFIMGGDTEYTAASAYRFDCETNQWTRIGDMTAKRMSCHAVASGNKLYVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA

Target ORF information:

RefSeq Version	NM_029652.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kelch-like 25 (Klhl25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029652.1

ORF Insert Sequence:

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl25 ( XM_011250832.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011249134.1 Mus musculus kelch-like 25 (Klhl25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05747
Clone ID Related Accession (Same CDS sequence)	NM_001122780.1 , NM_182782.2 , XM_011250832.2 , XM_011250832.3 , NM_029652.1
Accession Version	XM_011250832.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kelch-like protein 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011249134.1
CDS	209..1978
Translation	MSVSVHETRKSRSSTGSMNISVFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIVINEENAESLLEAGDMLQFHDVRDAAAEFLEKNLSPSNCLGMMVLSDAHQCRRLYEFSCRMSLVHFETVRQSEDFNSLSRDTLLDLISRDELETEDERVVFEAILQWVKHDLEQRKAHLPLLLRNVRLALLPSDCLKNAVSGEALLMADECTKLILDEAFRCKTKILLNDGVVTSPFARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGIFPASPSVSLKQVEKYDPGDNKWTMVAPMRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCFDPSENRWTIKAECPQPWRYTAAAVLGSQIFIMGGDTEYTAASAYRFDCETNQWTRIGDMTAKRMSCHAVASGNKLYVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA

Target ORF information:

RefSeq Version	XM_011250832.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kelch-like 25 (Klhl25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250832.2

ORF Insert Sequence:

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klhl25 ( XM_011250832.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011249134.1 Mus musculus kelch-like 25 (Klhl25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05747
Clone ID Related Accession (Same CDS sequence)	NM_001122780.1 , NM_182782.2 , XM_011250832.2 , XM_011250832.3 , NM_029652.1
Accession Version	XM_011250832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kelch-like protein 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011249134.1
CDS	238..2007
Translation	MSVSVHETRKSRSSTGSMNISVFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIVINEENAESLLEAGDMLQFHDVRDAAAEFLEKNLSPSNCLGMMVLSDAHQCRRLYEFSCRMSLVHFETVRQSEDFNSLSRDTLLDLISRDELETEDERVVFEAILQWVKHDLEQRKAHLPLLLRNVRLALLPSDCLKNAVSGEALLMADECTKLILDEAFRCKTKILLNDGVVTSPFARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGIFPASPSVSLKQVEKYDPGDNKWTMVAPMRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCFDPSENRWTIKAECPQPWRYTAAAVLGSQIFIMGGDTEYTAASAYRFDCETNQWTRIGDMTAKRMSCHAVASGNKLYVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA

Target ORF information:

RefSeq Version	XM_011250832.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kelch-like 25 (Klhl25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250832.3

ORF Insert Sequence:

ATGTCAGTCA GTGTTCACGA GACCCGCAAG TCGAGGAGCA GCACGGGCTC CATGAACATC 
TCAGTCTTCC ACAAGGCTTC ACACCCTGAC TGTGTGCTGG CCCACCTCAA CACTCTGCGC 
AAGCACTGCA TGTTCACGGA TGTCACACTC TGGGCTGGTG ACCGAGCCTT TCCTTGCCAT 
CGTGCGGTGC TGGCTGCCTC CAGCCGATAC TTTGAGGCCA TGTTCAGCCA CGGCCTGCGG 
GAAAGCCGGG ATGATACGGT CAACTTCCAG GACAACCTGC ACCCCGAGGT GTTGGAGCTG 
CTGTTGGACT TTGCCTACTC TTCTCGCATC GTCATCAATG AGGAGAACGC GGAGTCACTA 
CTAGAGGCTG GTGACATGCT GCAGTTCCAT GATGTCCGGG ATGCAGCTGC TGAGTTCCTG 
GAGAAGAATC TTTCCCCCTC CAACTGCCTT GGCATGATGG TGCTGTCTGA TGCCCACCAG 
TGCCGACGGC TCTACGAGTT CTCATGCCGA ATGTCCCTGG TGCATTTTGA GACGGTGCGG 
CAGAGCGAGG ACTTCAACAG TCTCTCTAGG GACACGCTGT TGGACCTCAT CTCCAGAGAT 
GAGCTGGAGA CTGAGGATGA GCGCGTGGTC TTTGAGGCCA TCCTGCAGTG GGTGAAGCAT 
GACTTAGAGC AGAGGAAGGC TCACCTGCCC CTGCTCCTGC GTAACGTGCG ACTGGCCCTG 
CTGCCCTCAG ACTGCCTGAA GAATGCCGTA TCTGGAGAGG CCCTCCTCAT GGCGGACGAG 
TGCACCAAGC TCATCTTAGA CGAGGCCTTC CGCTGCAAGA CCAAGATCTT GCTGAATGAT 
GGTGTGGTCA CCAGTCCCTT TGCCCGGCCT CGCAAGGCAG GTCACACACT GCTCATCTTG 
GGAGGCCAGA CCTTCATGTG TGACAAGATC TACCAAGTGG ACCACAAAGC CAAGGAGATT 
ATCCCCAAGG CAGATCTGCC AAGCCCCCGG AAGGAGTTCA GTGCTTCAGC GATTGGTTGC 
AAGGTCTATG TGACTGGAGG CAGGGGCTCT GAGAATGGAG TCTCTAAGGA TGTCTGGGTG 
TATGACACTG TCCATGAGGA ATGGTCTAAG GCCGCCCCTA TGCTGATTGC CCGCTTTGGC 
CATGGCTCAG CCGAGCTGGA GAACTGTCTC TATGTGGTGG GGGGGCATAC ATCCCTAGCA 
GGTATTTTCC CTGCCTCCCC TTCTGTCTCC CTGAAGCAAG TAGAGAAATA TGACCCTGGG 
GATAATAAGT GGACTATGGT GGCCCCCATG AGAGACGGTG TCAGCAATGC TGCAGTGGTG 
AGTGCCAAGC TGAAACTCTT TGTGTTTGGA GGGACCAGCA TCCACCGAGA TATGGTGTCC 
AAAGTCCAGT GTTTTGACCC CTCGGAGAAC CGGTGGACAA TCAAAGCAGA GTGTCCCCAG 
CCCTGGCGAT ACACAGCCGC CGCTGTCCTG GGCAGCCAGA TCTTCATCAT GGGAGGTGAT 
ACAGAGTACA CAGCAGCTTC AGCCTACCGC TTCGACTGTG AGACCAACCA GTGGACGCGG 
ATTGGGGACA TGACAGCCAA ACGCATGTCC TGTCATGCCG TGGCTTCAGG CAACAAGCTG 
TATGTGGTGG GAGGCTACTT TGGGACCCAG AGGTGTAAGA CCCTGGACTG CTATGACCCT 
ACTTCAGACA CATGGAACTG CATCACCACT GTGCCCTACT CTCTCATTCC CACGGCCTTT 
GTTAGCACCT GGAAGCATCT GCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)
Nishimura M,Yokoi N,Miki T,Horikawa Y,Yoshioka H,Takeda J,Ohara O,Seino S

Genomic analysis of mouse retinal development.
PLoS biology2(9)E247(2004 Sep)
Blackshaw S,Harpavat S,Trimarchi J,Cai L,Huang H,Kuo WP,Weber G,Lee K,Fraioli RE,Cho SH,Yung R,Asch E,Ohno-Machado L,Wong WH,Cepko CL

Genome-wide, large-scale production of mutant mice by ENU mutagenesis.
Nature genetics25(4)444-7(2000 Aug)
Hrab? de Angelis MH,Flaswinkel H,Fuchs H,Rathkolb B,Soewarto D,Marschall S,Heffner S,Pargent W,Wuensch K,Jung M,Reis A,Richter T,Alessandrini F,Jakob T,Fuchs E,Kolb H,Kremmer E,Schaeble K,Rollinski B,Roscher A,Peters C,Meitinger T,Strom T,Steckler T,Holsboer F,Klopstock T,Gekeler F,Schindewolf C,Jung T,Avraham K,Behrendt H,Ring J,Zimmer A,Schughart K,Pfeffer K,Wolf E,Balling R

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Klhl25 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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