Rs1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rs1
Entrez Gene ID	20147
Full Name	retinoschisis (X-linked, juvenile) 1 (human)
Synonyms	Rs1h,Xlrs1,tmgc1
General protein information	Preferred Names retinoschisis (X-linked, juvenile) 1 (human) Names retinoschisin X-linked juvenile retinoschisis protein homolog retinoschisis 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F4\|X 73.95 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030251270.1	XP_030107130.1	retinoschisin isoform X1
NM_011302.3	NP_035432.3	retinoschisin precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RS1	XP_548882.2
Rattus norvegicus (Norway rat)	Rs1	NP_001098113.1
Gallus gallus (chicken)	RS1	NP_001128447.1
Mus musculus (house mouse)	Rs1	NP_035432.3
Homo sapiens (human)	RS1	NP_000321.1
	RS1	XP_002806207.1
Xenopus tropicalis (tropical clawed frog)	rs1	NP_001096319.1
Danio rerio (zebrafish)	rs1a	NP_001003438.1

Gene Ontology
Bibliography

Related articles in PubMed

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.
Pl?ssl K, Royer M, Bernklau S, Tavraz NN, Friedrich T, Wild J, Weber BHF, Friedrich U
Molecular biology of the cell28(16)2178-2189(2017 Aug)

The X-linked juvenile retinoschisis protein retinoschisin is a novel regulator of mitogen-activated protein kinase signalling and apoptosis in the retina.
Pl?ssl K, Weber BH, Friedrich U
Journal of cellular and molecular medicine21(4)768-780(2017 04)

Rearing Light Intensity Affects Inner Retinal Pathology in a Mouse Model of X-Linked Retinoschisis but Does Not Alter Gene Therapy Outcome.
Marangoni D, Yong Z, Kjellstr?m S, Vijayasarathy C, A Sieving P, Bush RA
Investigative ophthalmology & visual science58(3)1656-1664(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest a regulatory effect of retinoschisin on Na/K-ATPase signaling and localization, whereas Na/K-ATPase-dysregulation caused by retinoschisin deficiency could represent an initial step in XLRS pathogenesis.
Title: Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

retinoschisin is a novel regulator of MAP kinase signalling and exerts an anti-apoptotic effect on retinal cells.
Title: The X-linked juvenile retinoschisis protein retinoschisin is a novel regulator of mitogen-activated protein kinase signalling and apoptosis in the retina.

Based on this structure, we propose that RS1 couples neighboring membranes together through octamer-octamer contacts, perhaps modulated by interactions with other membrane components.
Title: Paired octamer rings of retinoschisin suggest a junctional model for cell-cell adhesion in the retina.

Changes in Rs1-knockout mice were associated with age related alterations in photoreceptor morphology and transcription factor expression that suggest delayed photoreceptor maturation.
Title: Photoreceptor pathology in the X-linked retinoschisis (XLRS) mouse results in delayed rod maturation and impaired light driven transducin translocation.

The results of this study demonstrated that loss of Rs1 gene function has a significant impact on the expression of photoreceptor transcription factor network genes, and morphological and functional defects in young (P21) Rs1-KO mice.
Title: Loss of retinoschisin (RS1) cell surface protein in maturing mouse rod photoreceptors elevates the luminance threshold for light-driven translocation of transducin but not arrestin.

The following Rs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14464	NM_011302.3 Latest version!	Mus musculus retinoschisis (X-linked, juvenile) 1 (human) (Rs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu90371	XM_030251270.1 Latest version!	Mus musculus retinoschisis (X-linked, juvenile) 1 (human) (Rs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rs1 ( NM_011302.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035432.3 Mus musculus retinoschisis (X-linked, juvenile) 1 (human) (Rs1), mRNA.

Price & Availability↑

CloneID	OMu14464
Clone ID Related Accession (Same CDS sequence)	NM_011302.3
Accession Version	NM_011302.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	retinoschisin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC046422.1. On Feb 9, 2004 this sequence version replaced NM_011302.2. Sequence Note: An in-frame AUG is located 26 codons upstream of the annotated translation start site but is not being annotated as a start site since it would disrupt the signal peptide on NP_035432.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046422.1, AF084561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849390, SAMN01164131 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCACACA AGATTGAAGG CTTCTTCTTG TTACTTCTCT TTGGCTATGA AGCCACATTG 
GGATTGTCAT CGACAGAGGA TGAGGGTGAG GACCCCTGGT ACCAGAAAGC ATGCAAGTGT 
GATTGCCAGG TAGGAGCCAA TGCTCTGTGG TCTGCTGGAG CTACCTCCTT AGACTGTATT 
CCAGAATGCC CATATCACAA GCCCCTGGGT TTCGAGTCAG GGGAGGTCAC GCCAGATCAG 
ATCACTTGCT CCAACCCAGA GCAGTATGTG GGCTGGTATT CCTCATGGAC AGCAAACAAG 
GCCCGACTCA ACAGTCAAGG TTTTGGGTGT GCTTGGCTTT CCAAGTATCA GGACAGCAGC 
CAGTGGTTAC AGATAGATTT GAAGGAGATC AAGGTGATTT CGGGGATCCT GACCCAAGGA 
CGCTGTGACA TAGACGAGTG GGTGACCAAG TACAGTGTGC AGTATAGGAC TGATGAGCGC 
CTGAACTGGA TCTACTATAA GGATCAGACC GGAAACAATC GGGTCTTCTA TGGAAACTCA 
GACCGGAGTT CTACAGTTCA GAACTTACTC AGGCCCCCCA TCATTTCCCG CTTCATCCGA 
CTGATCCCTC TAGGCTGGCA TGTCCGAATT GCCATCCGGA TGGAGCTGCT TGAGTGTGCC 
AGCAAGTGTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035432.3
CDS	174..848
Translation	MPHKIEGFFLLLLFGYEATLGLSSTEDEGEDPWYQKACKCDCQVGANALWSAGATSLDCIPECPYHKPLGFESGEVTPDQITCSNPEQYVGWYSSWTANKARLNSQGFGCAWLSKYQDSSQWLQIDLKEIKVISGILTQGRCDIDEWVTKYSVQYRTDERLNWIYYKDQTGNNRVFYGNSDRSSTVQNLLRPPIISRFIRLIPLGWHVRIAIRMELLECASKCA

Target ORF information:

RefSeq Version	NM_011302.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus retinoschisis (X-linked, juvenile) 1 (human) (Rs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011302.3

ORF Insert Sequence:

ATGCCACACA AGATTGAAGG CTTCTTCTTG TTACTTCTCT TTGGCTATGA AGCCACATTG 
GGATTGTCAT CGACAGAGGA TGAGGGTGAG GACCCCTGGT ACCAGAAAGC ATGCAAGTGT 
GATTGCCAGG TAGGAGCCAA TGCTCTGTGG TCTGCTGGAG CTACCTCCTT AGACTGTATT 
CCAGAATGCC CATATCACAA GCCCCTGGGT TTCGAGTCAG GGGAGGTCAC GCCAGATCAG 
ATCACTTGCT CCAACCCAGA GCAGTATGTG GGCTGGTATT CCTCATGGAC AGCAAACAAG 
GCCCGACTCA ACAGTCAAGG TTTTGGGTGT GCTTGGCTTT CCAAGTATCA GGACAGCAGC 
CAGTGGTTAC AGATAGATTT GAAGGAGATC AAGGTGATTT CGGGGATCCT GACCCAAGGA 
CGCTGTGACA TAGACGAGTG GGTGACCAAG TACAGTGTGC AGTATAGGAC TGATGAGCGC 
CTGAACTGGA TCTACTATAA GGATCAGACC GGAAACAATC GGGTCTTCTA TGGAAACTCA 
GACCGGAGTT CTACAGTTCA GAACTTACTC AGGCCCCCCA TCATTTCCCG CTTCATCCGA 
CTGATCCCTC TAGGCTGGCA TGTCCGAATT GCCATCCGGA TGGAGCTGCT TGAGTGTGCC 
AGCAAGTGTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rs1 ( XM_030251270.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107130.1 Mus musculus retinoschisis (X-linked, juvenile) 1 (human) (Rs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu90371
Clone ID Related Accession (Same CDS sequence)	XM_030251270.1
Accession Version	XM_030251270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	retinoschisin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 29% of CDS bases ##RefSeq-Attributes-END##

ATGTGCCAGA GGCAGCGGTT AAACTGTAAC CGGCCTGGGG TAAACTCTAA CGGCCTGGCC 
GCTGCTGGGG GAAAGTTTGG AGTCACGAAC TTAGAGGGAC CCGGGCACCG GCTCAACCAC 
GGCTTCGGCT CTCCACTTCA CTTAGATCTT GCTGTGACCA AGGACAAGGA GAAAATGCCA 
CACAAGATTG AAGGCTTCTT CTTGTTACTT CTCTTTGGCT ATGAAGCCTT TGACTACGAC 
TTTTGTGACT TGTGCTCCTC TGGATGCTTG CAGGATGAGG GTGAGGACCC CTGGTACCAG 
AAAGCATGCA AGTGTGATTG CCAGGTAGGA GCCAATGCTC TGTGGTCTGC TGGAGCTACC 
TCCTTAGACT GTATTCCAGA ATGCCCATAT CACAAGCCCC TGGGTTTCGA GTCAGGGGAG 
GTCACGCCAG ATCAGATCAC TTGCTCCAAC CCAGAGCAGT ATGTGGGCTG GTATTCCTCA 
TGGACAGCAA ACAAGGCCCG ACTCAACAGT CAAGGTTTTG GGTGTGCTTG GCTTTCCAAG 
TATCAGGACA GCAGCCAGTG GTTACAGATA GATTTGAAGG AGATCAAGGT GATTTCGGGG 
ATCCTGACCC AAGGACGCTG TGACATAGAC GAGTGGGTGA CCAAGTACAG TGTGCAGTAT 
AGGACTGATG AGCGCCTGAA CTGGATCTAC TATAAGGATC AGACCGGAAA CAATCGGGTC 
TTCTATGGAA ACTCAGACCG GAGTTCTACA GTTCAGAACT TACTCAGGCC CCCCATCATT 
TCCCGCTTCA TCCGACTGAT CCCTCTAGGC TGGCATGTCC GAATTGCCAT CCGGATGGAG 
CTGCTTGAGT GTGCCAGCAA GTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107130.1
CDS	1..870
Translation	MCQRQRLNCNRPGVNSNGLAAAGGKFGVTNLEGPGHRLNHGFGSPLHLDLAVTKDKEKMPHKIEGFFLLLLFGYEAFDYDFCDLCSSGCLQDEGEDPWYQKACKCDCQVGANALWSAGATSLDCIPECPYHKPLGFESGEVTPDQITCSNPEQYVGWYSSWTANKARLNSQGFGCAWLSKYQDSSQWLQIDLKEIKVISGILTQGRCDIDEWVTKYSVQYRTDERLNWIYYKDQTGNNRVFYGNSDRSSTVQNLLRPPIISRFIRLIPLGWHVRIAIRMELLECASKCA

Target ORF information:

RefSeq Version	XM_030251270.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus retinoschisis (X-linked, juvenile) 1 (human) (Rs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251270.1

ORF Insert Sequence:

ATGTGCCAGA GGCAGCGGTT AAACTGTAAC CGGCCTGGGG TAAACTCTAA CGGCCTGGCC 
GCTGCTGGGG GAAAGTTTGG AGTCACGAAC TTAGAGGGAC CCGGGCACCG GCTCAACCAC 
GGCTTCGGCT CTCCACTTCA CTTAGATCTT GCTGTGACCA AGGACAAGGA GAAAATGCCA 
CACAAGATTG AAGGCTTCTT CTTGTTACTT CTCTTTGGCT ATGAAGCCTT TGACTACGAC 
TTTTGTGACT TGTGCTCCTC TGGATGCTTG CAGGATGAGG GTGAGGACCC CTGGTACCAG 
AAAGCATGCA AGTGTGATTG CCAGGTAGGA GCCAATGCTC TGTGGTCTGC TGGAGCTACC 
TCCTTAGACT GTATTCCAGA ATGCCCATAT CACAAGCCCC TGGGTTTCGA GTCAGGGGAG 
GTCACGCCAG ATCAGATCAC TTGCTCCAAC CCAGAGCAGT ATGTGGGCTG GTATTCCTCA 
TGGACAGCAA ACAAGGCCCG ACTCAACAGT CAAGGTTTTG GGTGTGCTTG GCTTTCCAAG 
TATCAGGACA GCAGCCAGTG GTTACAGATA GATTTGAAGG AGATCAAGGT GATTTCGGGG 
ATCCTGACCC AAGGACGCTG TGACATAGAC GAGTGGGTGA CCAAGTACAG TGTGCAGTAT 
AGGACTGATG AGCGCCTGAA CTGGATCTAC TATAAGGATC AGACCGGAAA CAATCGGGTC 
TTCTATGGAA ACTCAGACCG GAGTTCTACA GTTCAGAACT TACTCAGGCC CCCCATCATT 
TCCCGCTTCA TCCGACTGAT CCCTCTAGGC TGGCATGTCC GAATTGCCAT CCGGATGGAG 
CTGCTTGAGT GTGCCAGCAA GTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.
Molecular biology of the cell28(16)2178-2189(2017 Aug)
Pl?ssl K,Royer M,Bernklau S,Tavraz NN,Friedrich T,Wild J,Weber BHF,Friedrich U

The X-linked juvenile retinoschisis protein retinoschisin is a novel regulator of mitogen-activated protein kinase signalling and apoptosis in the retina.
Journal of cellular and molecular medicine21(4)768-780(2017 04)
Pl?ssl K,Weber BH,Friedrich U

Rearing Light Intensity Affects Inner Retinal Pathology in a Mouse Model of X-Linked Retinoschisis but Does Not Alter Gene Therapy Outcome.
Investigative ophthalmology & visual science58(3)1656-1664(2017 03)
Marangoni D,Yong Z,Kjellstr?m S,Vijayasarathy C,A Sieving P,Bush RA