Ret cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ret
Entrez Gene ID	19713
Full Name	ret proto-oncogene
Synonyms	PTC,RET51,RET9,c-Ret
General protein information	Preferred Names ret proto-oncogene Names proto-oncogene tyrosine-protein kinase receptor Ret proto-oncogene c-Ret
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F1\|6 55.86 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009050.2	NP_033076.2	proto-oncogene tyrosine-protein kinase receptor Ret isoform a precursor
NM_001080780.1	NP_001074249.1	proto-oncogene tyrosine-protein kinase receptor Ret isoform c precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RET	NP_001184028.1
Bos taurus (cattle)	RET	NP_001178412.1
Homo sapiens (human)	RET	NP_066124.1
	RET	XP_002805672.1
Rattus norvegicus (Norway rat)	Ret	NP_036775.2
Danio rerio (zebrafish)	ret	NP_858048.2
Pan troglodytes (chimpanzee)	RET	XP_003312612.1
Mus musculus (house mouse)	Ret	NP_033076.2
Xenopus tropicalis (tropical clawed frog)	ret	XP_002933941.2
Gallus gallus (chicken)	RET	NP_990521.1

Gene Ontology

The following Ret gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ret cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22240	NM_001080780.1 Latest version!	Mus musculus ret proto-oncogene (Ret), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu22368	NM_009050.2 Latest version!	Mus musculus ret proto-oncogene (Ret), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$657.30 $939.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ret ( NM_001080780.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074249.1 Mus musculus ret proto-oncogene (Ret), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu22240
Clone ID Related Accession (Same CDS sequence)	NM_001080780.1
Accession Version	NM_001080780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	proto-oncogene tyrosine-protein kinase receptor Ret isoform c precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC156396.8, BC059012.1 and AC135861.5. Transcript Variant: This variant (4) represents the longer transcript but encodes the shorter isoform (c). This isoform is also known as Ret9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC059012.1, AK084977.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGAAAG CGACGTCCGG CGCCGCAGGG CTGGGGCTGA AGCTGATTTT GCTCCTGCCG 
CTGCTAGGAG AAGCCCCACT GGGCCTCTAT TTCTCAAGGG ATGCTTACTG GGAGAGGCTG 
TATGTAGACC AGCCAGCTGG CACACCTCTG CTCTATGTCC ATGCCCTACG GGATGCCCCT 
GGAGAAGTGC CGAGCTTCCG CCTGGGCCAG CATCTCTATG GCGTCTACCG TACACGGCTG 
CATGAGAATG ACTGGATCCG CATCAATGAG ACTACTGGCC TTCTCTACCT CAATCAGAGC 
CTGGACCACA GTTCCTGGGA ACAGCTCAGC ATCCGCAATG GTGGTTTCCC CCTGCTCACC 
ATCTTCCTCC AGGTCTTTCT GGGGTCCACA GCCCAGAGAG AGGGAGAATG CCATTGGCCA 
GGCTGTACCC GTGTGTACTT CTCCTTCATC AACGACACCT TCCCAAATTG TAGCTCCTTC 
AAAGCCCAGG ATCTCTGCAT CCCAGAGACA GCCGTGTCCT TCCGAGTCAG GGAGAACAGG 
CCTCCTGGCA CCTTCTACCA CTTCCACATG TTACCCGTGC AGTTCCTTTG TCCCAACATC 
AGTGTGAAGT ACAGTCTCTT AGGAGGGGAT AGTCTGCCCT TCCGTTGTGA CCCAGACTGC 
CTGGAGGTGA GCACTCGCTG GGCCCTGGAT CGAGAGCTCC GGGAGAAGTA TGTGCTGGAG 
GCTTTGTGCA TAGTGGCAGG CCCTGGTGCC AACAAAGAGA CGGTGACTCT GTCCTTCCCA 
GTGACAGTGT ATGATGAGGA CGACTCGGCG CCCACCTTCT CTGGAGGTGT GGGCACTGCC 
AGCGCGGTGG TGGAGTTTAA GCGGAAGGAG GGCACTGTGG TGGCCACCCT GCAGGTGTTC 
GATGCAGATG TGGTGCCAGC GTCTGGGGAG CTGGTGAGAC GGTACACAAA CACACTCCTC 
TCAGGGGACT CCTGGGCCCA GCAGACCTTC CGGGTGGAGC ATTCGCCCAT CGAGACCTTG 
GTCCAGGTCA ACAACAACTC CGTTCGGGCA ACCATGCACA ATTACAAGCT GATTCTCAAC 
AGGAGCCTGT CTATCTCAGA GAGCCGAGTC CTGCAGCTCG CGGTCCTGGT CAACGACTCA 
GACTTCCAGG GGCCTGGGGC AGGTGGTATC CTCGTCCTCC ATTTCAACGT GTCTGTACTG 
CCCGTCACCC TGAACCTACC CAGGGCCTAC TCCTTCCCAG TGAATAAGAG GGCCCGCCGC 
TATGCCCAGA TCGGGAAAGT CTGTGTGGAA AACTGCCAGG AGTTCAGCGG TGTCTCCATC 
CAGTACAAGC TGCAGCCTTC CAGCATCAAC TGCACTGCCC TAGGTGTGGT CACCTCACCC 
GAGGACACCT CGGGGACCCT ATTTGTAAAT GACACAGAGG CCCTGCGGCG ACCTGAGTGC 
ACCAAGCTTC AGTACACGGT GGTAGCCACT GACCGGCAGA CCCGCAGACA GACCCAGGCT 
TCGCTAGTGG TCACTGTGGA GGGGACATCC ATTACTGAAG AAGTAGGCTG CCCCAAGTCC 
TGTGCAGTAA ACAAGAGGCG CCCCGAGTGT GAGGAATGTG GTGGCCTGGG TTCTCCAACT 
GGCAGGTGCG AGTGGCGCCA GGGAGATGGT AAAGGGATCA CCAGGAACTT CTCCACCTGC 
TCCCCCAGTA CCAGGACCTG CCCCGACGGC CACTGTGATG CTGTGGAGAG CCGGGATGCC 
AACATTTGCC CCCAGGACTG TCTCCGTGCC GACATTGTTG GAGGACACGA GCGAGGGGAG 
CGCCAGGGCA TTAAAGCAGG CTACGGCATC TGCAACTGTT TCCCTGATGA GAAGAAATGC 
TTCTGCGAGC CAGAGGACAG CCAGGGCCCA CTGTGTGATG CGCTGTGCCG CACGATCATC 
ACAGCTGCCC TCTTCTCCCT TATCATCTCC ATCCTGCTGT CCATCTTCTG TGTCTGCCAC 
CACCACAAGC ATGGGCACAA GCCGCCCATT GCATCAGCGG AAATGACCTT CTGCCGGCCG 
GCCCAGGGCT TCCCAATCAG TTATTCCTCC TCAGGCACCC GCCGGCCCTC ACTGGATTCC 
ACGGAGAACC AGGTTCCTGT GGACTCTTTC AAGATCCCGG AGGATCCAAA GTGGGAATTT 
CCTCGGAAGA ACTTAGTTCT TGGGAAAACT CTGGGAGAAG GCGAGTTTGG AAAAGTTGTC 
AAGGCCACAG CCTTCCGTCT GAAAGGCCGG GCAGGATACA CCACAGTGGC TGTGAAAATG 
CTGAAAGAAA ACGCCTCCCA GAGTGAGTTA CGAGACCTGC TGTCTGAGTT CAACCTTCTG 
AAACAAGTCA ACCATCCACA TGTCATCAAG TTGTATGGGG CCTGCAGCCA GGATGGGCCA 
CTTCTTCTCA TTGTGGAGTA TGCCAAGTAT GGCTCCCTGC GGGGATTCCT CCGTGACAGC 
CGCAAGATTG GGCCTGCCTA TGTGAGCGGT GGAGGCAGCC GCAACTCCAG CTCGCTGGAC 
CACCCAGATG AAAGGGTACT GACCATGGGT GACCTCATCT CCTTCGCCTG GCAGATCTCG 
AGGGGTATGC AGTACTTGGC AGAAATGAAG CTTGTACATC GGGACTTAGC TGCCAGGAAC 
ATCTTGGTGG CTGAGGGACG GAAGATGAAG ATTTCCGACT TTGGGCTGTC CCGAGATGTT 
TATGAGGAAG ATTCCTATGT GAAGAAAAGC AAGGGCCGGA TTCCCGTCAA GTGGATGGCA 
ATTGAGTCCC TTTTCGATCA CATCTATACT ACTCAAAGTG ATGTGTGGTC CTTTGGAGTG 
CTGCTCTGGG AGATTGTGAC CCTGGGAGGC AACCCCTACC CTGGAATTCC TCCTGAACGA 
CTCTTCAACC TTCTGAAGAC AGGCCACAGG ATGGAGAGGC CAGACAACTG CAGCGAGGAA 
ATGTACCGTC TGATGCTGCA GTGCTGGAAG CAGGAGCCAG ACAAGAGGCC AGTGTTTGCT 
GACATCAGCA AGGATCTGGA GAAGATGATG GTCAAGAGCA GAGACTACTT GGACCTGGCT 
GCATCCACAC CTTCGGACTC ACTGCTGTAT GACGATGGGC TCTCAGAAGA GGAGACACCC 
CTGGTGGACT GTAACAATGC TCCCCTCCCG CGCTCCCTCC CTTCCACATG GATTGAAAAC 
AAACTCTATG GTAGAATTTC ACATGCATTT ACTAGATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074249.1
CDS	612..3833
Translation	MAKATSGAAGLGLKLILLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQHLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWPGCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSFRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGDSLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTASAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRATMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARRYAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVATDRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPLCDALCRTIITAALFSLIISILLSIFCVCHHHKHGHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDSTENQVPVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSGGGSRNSSSLDHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLPRSLPSTWIENKLYGRISHAFTRF

Target ORF information:

RefSeq Version	NM_001080780.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ret proto-oncogene (Ret), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080780.1

ORF Insert Sequence:

ATGGCGAAAG CGACGTCCGG CGCCGCAGGG CTGGGGCTGA AGCTGATTTT GCTCCTGCCG 
CTGCTAGGAG AAGCCCCACT GGGCCTCTAT TTCTCAAGGG ATGCTTACTG GGAGAGGCTG 
TATGTAGACC AGCCAGCTGG CACACCTCTG CTCTATGTCC ATGCCCTACG GGATGCCCCT 
GGAGAAGTGC CGAGCTTCCG CCTGGGCCAG CATCTCTATG GCGTCTACCG TACACGGCTG 
CATGAGAATG ACTGGATCCG CATCAATGAG ACTACTGGCC TTCTCTACCT CAATCAGAGC 
CTGGACCACA GTTCCTGGGA ACAGCTCAGC ATCCGCAATG GTGGTTTCCC CCTGCTCACC 
ATCTTCCTCC AGGTCTTTCT GGGGTCCACA GCCCAGAGAG AGGGAGAATG CCATTGGCCA 
GGCTGTACCC GTGTGTACTT CTCCTTCATC AACGACACCT TCCCAAATTG TAGCTCCTTC 
AAAGCCCAGG ATCTCTGCAT CCCAGAGACA GCCGTGTCCT TCCGAGTCAG GGAGAACAGG 
CCTCCTGGCA CCTTCTACCA CTTCCACATG TTACCCGTGC AGTTCCTTTG TCCCAACATC 
AGTGTGAAGT ACAGTCTCTT AGGAGGGGAT AGTCTGCCCT TCCGTTGTGA CCCAGACTGC 
CTGGAGGTGA GCACTCGCTG GGCCCTGGAT CGAGAGCTCC GGGAGAAGTA TGTGCTGGAG 
GCTTTGTGCA TAGTGGCAGG CCCTGGTGCC AACAAAGAGA CGGTGACTCT GTCCTTCCCA 
GTGACAGTGT ATGATGAGGA CGACTCGGCG CCCACCTTCT CTGGAGGTGT GGGCACTGCC 
AGCGCGGTGG TGGAGTTTAA GCGGAAGGAG GGCACTGTGG TGGCCACCCT GCAGGTGTTC 
GATGCAGATG TGGTGCCAGC GTCTGGGGAG CTGGTGAGAC GGTACACAAA CACACTCCTC 
TCAGGGGACT CCTGGGCCCA GCAGACCTTC CGGGTGGAGC ATTCGCCCAT CGAGACCTTG 
GTCCAGGTCA ACAACAACTC CGTTCGGGCA ACCATGCACA ATTACAAGCT GATTCTCAAC 
AGGAGCCTGT CTATCTCAGA GAGCCGAGTC CTGCAGCTCG CGGTCCTGGT CAACGACTCA 
GACTTCCAGG GGCCTGGGGC AGGTGGTATC CTCGTCCTCC ATTTCAACGT GTCTGTACTG 
CCCGTCACCC TGAACCTACC CAGGGCCTAC TCCTTCCCAG TGAATAAGAG GGCCCGCCGC 
TATGCCCAGA TCGGGAAAGT CTGTGTGGAA AACTGCCAGG AGTTCAGCGG TGTCTCCATC 
CAGTACAAGC TGCAGCCTTC CAGCATCAAC TGCACTGCCC TAGGTGTGGT CACCTCACCC 
GAGGACACCT CGGGGACCCT ATTTGTAAAT GACACAGAGG CCCTGCGGCG ACCTGAGTGC 
ACCAAGCTTC AGTACACGGT GGTAGCCACT GACCGGCAGA CCCGCAGACA GACCCAGGCT 
TCGCTAGTGG TCACTGTGGA GGGGACATCC ATTACTGAAG AAGTAGGCTG CCCCAAGTCC 
TGTGCAGTAA ACAAGAGGCG CCCCGAGTGT GAGGAATGTG GTGGCCTGGG TTCTCCAACT 
GGCAGGTGCG AGTGGCGCCA GGGAGATGGT AAAGGGATCA CCAGGAACTT CTCCACCTGC 
TCCCCCAGTA CCAGGACCTG CCCCGACGGC CACTGTGATG CTGTGGAGAG CCGGGATGCC 
AACATTTGCC CCCAGGACTG TCTCCGTGCC GACATTGTTG GAGGACACGA GCGAGGGGAG 
CGCCAGGGCA TTAAAGCAGG CTACGGCATC TGCAACTGTT TCCCTGATGA GAAGAAATGC 
TTCTGCGAGC CAGAGGACAG CCAGGGCCCA CTGTGTGATG CGCTGTGCCG CACGATCATC 
ACAGCTGCCC TCTTCTCCCT TATCATCTCC ATCCTGCTGT CCATCTTCTG TGTCTGCCAC 
CACCACAAGC ATGGGCACAA GCCGCCCATT GCATCAGCGG AAATGACCTT CTGCCGGCCG 
GCCCAGGGCT TCCCAATCAG TTATTCCTCC TCAGGCACCC GCCGGCCCTC ACTGGATTCC 
ACGGAGAACC AGGTTCCTGT GGACTCTTTC AAGATCCCGG AGGATCCAAA GTGGGAATTT 
CCTCGGAAGA ACTTAGTTCT TGGGAAAACT CTGGGAGAAG GCGAGTTTGG AAAAGTTGTC 
AAGGCCACAG CCTTCCGTCT GAAAGGCCGG GCAGGATACA CCACAGTGGC TGTGAAAATG 
CTGAAAGAAA ACGCCTCCCA GAGTGAGTTA CGAGACCTGC TGTCTGAGTT CAACCTTCTG 
AAACAAGTCA ACCATCCACA TGTCATCAAG TTGTATGGGG CCTGCAGCCA GGATGGGCCA 
CTTCTTCTCA TTGTGGAGTA TGCCAAGTAT GGCTCCCTGC GGGGATTCCT CCGTGACAGC 
CGCAAGATTG GGCCTGCCTA TGTGAGCGGT GGAGGCAGCC GCAACTCCAG CTCGCTGGAC 
CACCCAGATG AAAGGGTACT GACCATGGGT GACCTCATCT CCTTCGCCTG GCAGATCTCG 
AGGGGTATGC AGTACTTGGC AGAAATGAAG CTTGTACATC GGGACTTAGC TGCCAGGAAC 
ATCTTGGTGG CTGAGGGACG GAAGATGAAG ATTTCCGACT TTGGGCTGTC CCGAGATGTT 
TATGAGGAAG ATTCCTATGT GAAGAAAAGC AAGGGCCGGA TTCCCGTCAA GTGGATGGCA 
ATTGAGTCCC TTTTCGATCA CATCTATACT ACTCAAAGTG ATGTGTGGTC CTTTGGAGTG 
CTGCTCTGGG AGATTGTGAC CCTGGGAGGC AACCCCTACC CTGGAATTCC TCCTGAACGA 
CTCTTCAACC TTCTGAAGAC AGGCCACAGG ATGGAGAGGC CAGACAACTG CAGCGAGGAA 
ATGTACCGTC TGATGCTGCA GTGCTGGAAG CAGGAGCCAG ACAAGAGGCC AGTGTTTGCT 
GACATCAGCA AGGATCTGGA GAAGATGATG GTCAAGAGCA GAGACTACTT GGACCTGGCT 
GCATCCACAC CTTCGGACTC ACTGCTGTAT GACGATGGGC TCTCAGAAGA GGAGACACCC 
CTGGTGGACT GTAACAATGC TCCCCTCCCG CGCTCCCTCC CTTCCACATG GATTGAAAAC 
AAACTCTATG GTAGAATTTC ACATGCATTT ACTAGATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ret ( NM_009050.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033076.2 Mus musculus ret proto-oncogene (Ret), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22368
Clone ID Related Accession (Same CDS sequence)	NM_009050.2
Accession Version	NM_009050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	proto-oncogene tyrosine-protein kinase receptor Ret isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC156396.8, BC059012.1 and AC135861.5. On Jan 29, 2007 this sequence version replaced NM_009050.1. Transcript Variant: This variant (2) lacks an alternate segment in the 3' coding region, compared to variant 4. The resulting protein (isoform a) has a longer, distinct C-terminus compared to isoform c. This isoform is also known as Ret51. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF209436.1, X67812.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGAAAG CGACGTCCGG CGCCGCAGGG CTGGGGCTGA AGCTGATTTT GCTCCTGCCG 
CTGCTAGGAG AAGCCCCACT GGGCCTCTAT TTCTCAAGGG ATGCTTACTG GGAGAGGCTG 
TATGTAGACC AGCCAGCTGG CACACCTCTG CTCTATGTCC ATGCCCTACG GGATGCCCCT 
GGAGAAGTGC CGAGCTTCCG CCTGGGCCAG CATCTCTATG GCGTCTACCG TACACGGCTG 
CATGAGAATG ACTGGATCCG CATCAATGAG ACTACTGGCC TTCTCTACCT CAATCAGAGC 
CTGGACCACA GTTCCTGGGA ACAGCTCAGC ATCCGCAATG GTGGTTTCCC CCTGCTCACC 
ATCTTCCTCC AGGTCTTTCT GGGGTCCACA GCCCAGAGAG AGGGAGAATG CCATTGGCCA 
GGCTGTACCC GTGTGTACTT CTCCTTCATC AACGACACCT TCCCAAATTG TAGCTCCTTC 
AAAGCCCAGG ATCTCTGCAT CCCAGAGACA GCCGTGTCCT TCCGAGTCAG GGAGAACAGG 
CCTCCTGGCA CCTTCTACCA CTTCCACATG TTACCCGTGC AGTTCCTTTG TCCCAACATC 
AGTGTGAAGT ACAGTCTCTT AGGAGGGGAT AGTCTGCCCT TCCGTTGTGA CCCAGACTGC 
CTGGAGGTGA GCACTCGCTG GGCCCTGGAT CGAGAGCTCC GGGAGAAGTA TGTGCTGGAG 
GCTTTGTGCA TAGTGGCAGG CCCTGGTGCC AACAAAGAGA CGGTGACTCT GTCCTTCCCA 
GTGACAGTGT ATGATGAGGA CGACTCGGCG CCCACCTTCT CTGGAGGTGT GGGCACTGCC 
AGCGCGGTGG TGGAGTTTAA GCGGAAGGAG GGCACTGTGG TGGCCACCCT GCAGGTGTTC 
GATGCAGATG TGGTGCCAGC GTCTGGGGAG CTGGTGAGAC GGTACACAAA CACACTCCTC 
TCAGGGGACT CCTGGGCCCA GCAGACCTTC CGGGTGGAGC ATTCGCCCAT CGAGACCTTG 
GTCCAGGTCA ACAACAACTC CGTTCGGGCA ACCATGCACA ATTACAAGCT GATTCTCAAC 
AGGAGCCTGT CTATCTCAGA GAGCCGAGTC CTGCAGCTCG CGGTCCTGGT CAACGACTCA 
GACTTCCAGG GGCCTGGGGC AGGTGGTATC CTCGTCCTCC ATTTCAACGT GTCTGTACTG 
CCCGTCACCC TGAACCTACC CAGGGCCTAC TCCTTCCCAG TGAATAAGAG GGCCCGCCGC 
TATGCCCAGA TCGGGAAAGT CTGTGTGGAA AACTGCCAGG AGTTCAGCGG TGTCTCCATC 
CAGTACAAGC TGCAGCCTTC CAGCATCAAC TGCACTGCCC TAGGTGTGGT CACCTCACCC 
GAGGACACCT CGGGGACCCT ATTTGTAAAT GACACAGAGG CCCTGCGGCG ACCTGAGTGC 
ACCAAGCTTC AGTACACGGT GGTAGCCACT GACCGGCAGA CCCGCAGACA GACCCAGGCT 
TCGCTAGTGG TCACTGTGGA GGGGACATCC ATTACTGAAG AAGTAGGCTG CCCCAAGTCC 
TGTGCAGTAA ACAAGAGGCG CCCCGAGTGT GAGGAATGTG GTGGCCTGGG TTCTCCAACT 
GGCAGGTGCG AGTGGCGCCA GGGAGATGGT AAAGGGATCA CCAGGAACTT CTCCACCTGC 
TCCCCCAGTA CCAGGACCTG CCCCGACGGC CACTGTGATG CTGTGGAGAG CCGGGATGCC 
AACATTTGCC CCCAGGACTG TCTCCGTGCC GACATTGTTG GAGGACACGA GCGAGGGGAG 
CGCCAGGGCA TTAAAGCAGG CTACGGCATC TGCAACTGTT TCCCTGATGA GAAGAAATGC 
TTCTGCGAGC CAGAGGACAG CCAGGGCCCA CTGTGTGATG CGCTGTGCCG CACGATCATC 
ACAGCTGCCC TCTTCTCCCT TATCATCTCC ATCCTGCTGT CCATCTTCTG TGTCTGCCAC 
CACCACAAGC ATGGGCACAA GCCGCCCATT GCATCAGCGG AAATGACCTT CTGCCGGCCG 
GCCCAGGGCT TCCCAATCAG TTATTCCTCC TCAGGCACCC GCCGGCCCTC ACTGGATTCC 
ACGGAGAACC AGGTTCCTGT GGACTCTTTC AAGATCCCGG AGGATCCAAA GTGGGAATTT 
CCTCGGAAGA ACTTAGTTCT TGGGAAAACT CTGGGAGAAG GCGAGTTTGG AAAAGTTGTC 
AAGGCCACAG CCTTCCGTCT GAAAGGCCGG GCAGGATACA CCACAGTGGC TGTGAAAATG 
CTGAAAGAAA ACGCCTCCCA GAGTGAGTTA CGAGACCTGC TGTCTGAGTT CAACCTTCTG 
AAACAAGTCA ACCATCCACA TGTCATCAAG TTGTATGGGG CCTGCAGCCA GGATGGGCCA 
CTTCTTCTCA TTGTGGAGTA TGCCAAGTAT GGCTCCCTGC GGGGATTCCT CCGTGACAGC 
CGCAAGATTG GGCCTGCCTA TGTGAGCGGT GGAGGCAGCC GCAACTCCAG CTCGCTGGAC 
CACCCAGATG AAAGGGTACT GACCATGGGT GACCTCATCT CCTTCGCCTG GCAGATCTCG 
AGGGGTATGC AGTACTTGGC AGAAATGAAG CTTGTACATC GGGACTTAGC TGCCAGGAAC 
ATCTTGGTGG CTGAGGGACG GAAGATGAAG ATTTCCGACT TTGGGCTGTC CCGAGATGTT 
TATGAGGAAG ATTCCTATGT GAAGAAAAGC AAGGGCCGGA TTCCCGTCAA GTGGATGGCA 
ATTGAGTCCC TTTTCGATCA CATCTATACT ACTCAAAGTG ATGTGTGGTC CTTTGGAGTG 
CTGCTCTGGG AGATTGTGAC CCTGGGAGGC AACCCCTACC CTGGAATTCC TCCTGAACGA 
CTCTTCAACC TTCTGAAGAC AGGCCACAGG ATGGAGAGGC CAGACAACTG CAGCGAGGAA 
ATGTACCGTC TGATGCTGCA GTGCTGGAAG CAGGAGCCAG ACAAGAGGCC AGTGTTTGCT 
GACATCAGCA AGGATCTGGA GAAGATGATG GTCAAGAGCA GAGACTACTT GGACCTGGCT 
GCATCCACAC CTTCGGACTC ACTGCTGTAT GACGATGGGC TCTCAGAAGA GGAGACACCC 
CTGGTGGACT GTAACAATGC TCCCCTCCCG CGCTCCCTCC CTTCCACATG GATTGAAAAC 
AAACTCTATG GCATGTCAGA CCCGAACTGG CCTGGAGAGA GTCCTGTACC ACTCACGAGA 
GCCGATGGCA CTAGCACTGG GTTCCCAAGA TATGCAAATG ATAGTGTATA TGCTAACTGG 
ATGGTTTCAC CCTCAGCGGC AAAATTAATG GACACATTTG ATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033076.2
CDS	612..3959
Translation	MAKATSGAAGLGLKLILLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQHLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWPGCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSFRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGDSLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTASAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRATMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARRYAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVATDRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPLCDALCRTIITAALFSLIISILLSIFCVCHHHKHGHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDSTENQVPVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSGGGSRNSSSLDHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLPRSLPSTWIENKLYGMSDPNWPGESPVPLTRADGTSTGFPRYANDSVYANWMVSPSAAKLMDTFDS

Target ORF information:

RefSeq Version	NM_009050.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ret proto-oncogene (Ret), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009050.2

ORF Insert Sequence:

ATGGCGAAAG CGACGTCCGG CGCCGCAGGG CTGGGGCTGA AGCTGATTTT GCTCCTGCCG 
CTGCTAGGAG AAGCCCCACT GGGCCTCTAT TTCTCAAGGG ATGCTTACTG GGAGAGGCTG 
TATGTAGACC AGCCAGCTGG CACACCTCTG CTCTATGTCC ATGCCCTACG GGATGCCCCT 
GGAGAAGTGC CGAGCTTCCG CCTGGGCCAG CATCTCTATG GCGTCTACCG TACACGGCTG 
CATGAGAATG ACTGGATCCG CATCAATGAG ACTACTGGCC TTCTCTACCT CAATCAGAGC 
CTGGACCACA GTTCCTGGGA ACAGCTCAGC ATCCGCAATG GTGGTTTCCC CCTGCTCACC 
ATCTTCCTCC AGGTCTTTCT GGGGTCCACA GCCCAGAGAG AGGGAGAATG CCATTGGCCA 
GGCTGTACCC GTGTGTACTT CTCCTTCATC AACGACACCT TCCCAAATTG TAGCTCCTTC 
AAAGCCCAGG ATCTCTGCAT CCCAGAGACA GCCGTGTCCT TCCGAGTCAG GGAGAACAGG 
CCTCCTGGCA CCTTCTACCA CTTCCACATG TTACCCGTGC AGTTCCTTTG TCCCAACATC 
AGTGTGAAGT ACAGTCTCTT AGGAGGGGAT AGTCTGCCCT TCCGTTGTGA CCCAGACTGC 
CTGGAGGTGA GCACTCGCTG GGCCCTGGAT CGAGAGCTCC GGGAGAAGTA TGTGCTGGAG 
GCTTTGTGCA TAGTGGCAGG CCCTGGTGCC AACAAAGAGA CGGTGACTCT GTCCTTCCCA 
GTGACAGTGT ATGATGAGGA CGACTCGGCG CCCACCTTCT CTGGAGGTGT GGGCACTGCC 
AGCGCGGTGG TGGAGTTTAA GCGGAAGGAG GGCACTGTGG TGGCCACCCT GCAGGTGTTC 
GATGCAGATG TGGTGCCAGC GTCTGGGGAG CTGGTGAGAC GGTACACAAA CACACTCCTC 
TCAGGGGACT CCTGGGCCCA GCAGACCTTC CGGGTGGAGC ATTCGCCCAT CGAGACCTTG 
GTCCAGGTCA ACAACAACTC CGTTCGGGCA ACCATGCACA ATTACAAGCT GATTCTCAAC 
AGGAGCCTGT CTATCTCAGA GAGCCGAGTC CTGCAGCTCG CGGTCCTGGT CAACGACTCA 
GACTTCCAGG GGCCTGGGGC AGGTGGTATC CTCGTCCTCC ATTTCAACGT GTCTGTACTG 
CCCGTCACCC TGAACCTACC CAGGGCCTAC TCCTTCCCAG TGAATAAGAG GGCCCGCCGC 
TATGCCCAGA TCGGGAAAGT CTGTGTGGAA AACTGCCAGG AGTTCAGCGG TGTCTCCATC 
CAGTACAAGC TGCAGCCTTC CAGCATCAAC TGCACTGCCC TAGGTGTGGT CACCTCACCC 
GAGGACACCT CGGGGACCCT ATTTGTAAAT GACACAGAGG CCCTGCGGCG ACCTGAGTGC 
ACCAAGCTTC AGTACACGGT GGTAGCCACT GACCGGCAGA CCCGCAGACA GACCCAGGCT 
TCGCTAGTGG TCACTGTGGA GGGGACATCC ATTACTGAAG AAGTAGGCTG CCCCAAGTCC 
TGTGCAGTAA ACAAGAGGCG CCCCGAGTGT GAGGAATGTG GTGGCCTGGG TTCTCCAACT 
GGCAGGTGCG AGTGGCGCCA GGGAGATGGT AAAGGGATCA CCAGGAACTT CTCCACCTGC 
TCCCCCAGTA CCAGGACCTG CCCCGACGGC CACTGTGATG CTGTGGAGAG CCGGGATGCC 
AACATTTGCC CCCAGGACTG TCTCCGTGCC GACATTGTTG GAGGACACGA GCGAGGGGAG 
CGCCAGGGCA TTAAAGCAGG CTACGGCATC TGCAACTGTT TCCCTGATGA GAAGAAATGC 
TTCTGCGAGC CAGAGGACAG CCAGGGCCCA CTGTGTGATG CGCTGTGCCG CACGATCATC 
ACAGCTGCCC TCTTCTCCCT TATCATCTCC ATCCTGCTGT CCATCTTCTG TGTCTGCCAC 
CACCACAAGC ATGGGCACAA GCCGCCCATT GCATCAGCGG AAATGACCTT CTGCCGGCCG 
GCCCAGGGCT TCCCAATCAG TTATTCCTCC TCAGGCACCC GCCGGCCCTC ACTGGATTCC 
ACGGAGAACC AGGTTCCTGT GGACTCTTTC AAGATCCCGG AGGATCCAAA GTGGGAATTT 
CCTCGGAAGA ACTTAGTTCT TGGGAAAACT CTGGGAGAAG GCGAGTTTGG AAAAGTTGTC 
AAGGCCACAG CCTTCCGTCT GAAAGGCCGG GCAGGATACA CCACAGTGGC TGTGAAAATG 
CTGAAAGAAA ACGCCTCCCA GAGTGAGTTA CGAGACCTGC TGTCTGAGTT CAACCTTCTG 
AAACAAGTCA ACCATCCACA TGTCATCAAG TTGTATGGGG CCTGCAGCCA GGATGGGCCA 
CTTCTTCTCA TTGTGGAGTA TGCCAAGTAT GGCTCCCTGC GGGGATTCCT CCGTGACAGC 
CGCAAGATTG GGCCTGCCTA TGTGAGCGGT GGAGGCAGCC GCAACTCCAG CTCGCTGGAC 
CACCCAGATG AAAGGGTACT GACCATGGGT GACCTCATCT CCTTCGCCTG GCAGATCTCG 
AGGGGTATGC AGTACTTGGC AGAAATGAAG CTTGTACATC GGGACTTAGC TGCCAGGAAC 
ATCTTGGTGG CTGAGGGACG GAAGATGAAG ATTTCCGACT TTGGGCTGTC CCGAGATGTT 
TATGAGGAAG ATTCCTATGT GAAGAAAAGC AAGGGCCGGA TTCCCGTCAA GTGGATGGCA 
ATTGAGTCCC TTTTCGATCA CATCTATACT ACTCAAAGTG ATGTGTGGTC CTTTGGAGTG 
CTGCTCTGGG AGATTGTGAC CCTGGGAGGC AACCCCTACC CTGGAATTCC TCCTGAACGA 
CTCTTCAACC TTCTGAAGAC AGGCCACAGG ATGGAGAGGC CAGACAACTG CAGCGAGGAA 
ATGTACCGTC TGATGCTGCA GTGCTGGAAG CAGGAGCCAG ACAAGAGGCC AGTGTTTGCT 
GACATCAGCA AGGATCTGGA GAAGATGATG GTCAAGAGCA GAGACTACTT GGACCTGGCT 
GCATCCACAC CTTCGGACTC ACTGCTGTAT GACGATGGGC TCTCAGAAGA GGAGACACCC 
CTGGTGGACT GTAACAATGC TCCCCTCCCG CGCTCCCTCC CTTCCACATG GATTGAAAAC 
AAACTCTATG GCATGTCAGA CCCGAACTGG CCTGGAGAGA GTCCTGTACC ACTCACGAGA 
GCCGATGGCA CTAGCACTGG GTTCCCAAGA TATGCAAATG ATAGTGTATA TGCTAACTGG 
ATGGTTTCAC CCTCAGCGGC AAAATTAATG GACACATTTG ATAGCTAA