Ppm1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppm1a
Entrez Gene ID	19042
Full Name	protein phosphatase 1A, magnesium dependent, alpha isoform
Synonyms	2310003C21Rik,2900017D14Rik,AI427932,AU017636,MMPa-2,MPPa-1
General protein information	Preferred Names protein phosphatase 1A, magnesium dependent, alpha isoform Names protein phosphatase 1A Mpp alpha PP2C-alpha protein phosphatase 2C isoform alpha protein phosphatase IA
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515603.2	XP_006515666.1	protein phosphatase 1A isoform X1
XM_006515603.3	XP_006515666.1	protein phosphatase 1A isoform X1
NM_008910.3	NP_032936.1	protein phosphatase 1A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPM1A	XP_001167412.2
Canis lupus familiaris (dog)	PPM1A	XP_537467.3
Danio rerio (zebrafish)	ppm1aa	NP_571504.1
Homo sapiens (human)	PPM1A	NP_808821.2
Gallus gallus (chicken)	PPM1A	XP_421422.1
Xenopus tropicalis (tropical clawed frog)	ppm1a	NP_001016158.2
Rattus norvegicus (Norway rat)	Ppm1a	NP_058734.1
	PPM1A	XP_001096358.2
Bos taurus (cattle)	PPM1A	NP_776854.1
Mus musculus (house mouse)	Ppm1a	NP_032936.1

Gene Ontology
Bibliography

Related articles in PubMed

Glucose elicits serine/threonine kinase VRK1 to phosphorylate nuclear pregnane X receptor as a novel hepatic gluconeogenic signal.
Gotoh S, Miyauchi Y, Moore R, Negishi M
Cellular signalling40200-209(2017 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

PPM1A silences cytosolic RNA sensing and antiviral defense through direct dephosphorylation of MAVS and TBK1.
Xiang W, Zhang Q, Lin X, Wu S, Zhou Y, Meng F, Fan Y, Shen T, Xiao M, Xia Z, Zou J, Feng XH, Xu P
Science advances2(7)e1501889(2016 07)

Foxf2 Is Required for Brain Pericyte Differentiation and Development and Maintenance of the Blood-Brain Barrier.
Reyahi A, Nik AM, Ghiami M, Gritli-Linde A, Pont?n F, Johansson BR, Carlsson P
Developmental cell34(1)19-32(2015 Jul)

PPM1A regulates antiviral signaling by antagonizing TBK1-mediated STING phosphorylation and aggregation.
Li Z, Liu G, Sun L, Teng Y, Guo X, Jia J, Sha J, Yang X, Chen D, Sun Q
PLoS pathogens11(3)e1004783(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings demonstrate a novel regulatory circuit in which STING and TBK1 reciprocally regulate each other to enable efficient antiviral signaling activation, and PPM1A dephosphorylates STING and TBK1
Title: PPM1A regulates antiviral signaling by antagonizing TBK1-mediated STING phosphorylation and aggregation.

the studies presented here position PPM1A as a new player in the wound healing-inflammation-angiogenesis axis in mouse, reveal its crucial role in homeostasis on injury, and highlight its potential as a therapeutic mediator in pathologic conditions
Title: Protein phosphatase magnesium dependent 1A governs the wound healing-inflammation-angiogenesis cross talk on injury.

a nuclear envelope-localized mechanism of inactivating TGF-beta signaling in which MAN1 competes with transcription factors for binding to Smad2 and Smad3 and facilitates their dephosphorylation by PPM1A.
Title: Inhibition of TGF-? signaling at the nuclear envelope: characterization of interactions between MAN1, Smad2 and Smad3, and PPM1A.

Although a non-myristoylated mutation (G2A) of PPM1A and PPM1B prevented membrane association, this relocalization did not likely cause the decreased activity towards AMPKalpha.
Title: N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells.

we found that PPM1A mRNA is synthesized at the beginning of the maturation process and remains elevated in the mature oocytes, promoting the accumulation of PPM1A protein
Title: De novo synthesis of protein phosphatase 1A, magnesium dependent, alpha isoform (PPM1A) during oocyte maturation.

The following Ppm1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppm1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03069	NM_008910.3 Latest version!	Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu03069	XM_006515603.3 Latest version!	Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OMu03069	XM_006515603.2	Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppm1a ( NM_008910.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032936.1 Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), mRNA.

Price & Availability↑

CloneID	OMu03069
Clone ID Related Accession (Same CDS sequence)	XM_006515603.2 , XM_006515603.3 , NM_008910.3
Accession Version	NM_008910.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY739448.1, AK076054.1, D28117.1, BE861326.1, AV307012.2 and BM932496.1. On Aug 11, 2009 this sequence version replaced NM_008910.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008595.1, SRR7652917.131269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAGAAGCATA ATGCCCAGGG GCAGGGGAAT 
GGGTTACGAT ACGGCCTAAG CAGCATGCAA GGTTGGCGAG TTGAAATGGA GGACGCACAC 
ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAGACATGGT CATTCTTTGC TGTATATGAT 
GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ACTTGTTAGA TCACATCACC 
AATAACCAGG ATTTCAGAGG ATCTGCAGGA GCACCTTCTG TGGAGAACGT AAAGAATGGA 
ATCAGAACAG GGTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 
GGTGCAGATA GAAGCGGGTC AACAGCTGTG GGCGTCTTAA TCTCTCCCCA ACATACTTAT 
TTCATTAACT GTGGAGACTC GAGAGGTTTA CTTTGTAGGA ATAGAAAAGT TCACTTCTTC 
ACACAAGACC ATAAACCAAG TAACCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGGGGC 
TCGGTGATGA TTCAGCGTGT CAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTC 
GATTACAAAT GTGTCCATGG AAAAGGTCCC ACAGAGCAGC TTGTCTCCCC AGAGCCCGAA 
GTCCATGATA TTGAAAGGTC TGAAGAAGAT GACCAGTTCA TCATCCTTGC ATGCGATGGC 
ATCTGGGACG TCATGGGGAA CGAAGAGCTC TGTGACTTTG TGAGATCCAG ACTTGAAGTC 
ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGCTTGTA TAAGGGAAGT 
CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAGTG CACCCAAAGT CTCGGCAGAG 
GCGGTGAAGA AGGAGGCGGA GCTGGACAAG TACCTGGAGA GCAGAGTAGA AGAAATCATA 
AAGAAGCAGG TGGAAGGCGT CCCTGACTTA GTCCACGTGA TGCGCACGTT AGCCAGTGAG 
AACATCCCCA GCCTCCCACC AGGGGGTGAA TTGGCAAGCA AGCGGAATGT AATTGAAGCC 
GTTTACAATA GACTGAACCC TTACAAAAAT GACGACACTG ATTCTGCGTC AACCGATGAT 
ATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032936.1
CDS	442..1590
Translation	MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSASTDDMW

Target ORF information:

RefSeq Version	NM_008910.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008910.3

ORF Insert Sequence:

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAGAAGCATA ATGCCCAGGG GCAGGGGAAT 
GGGTTACGAT ACGGCCTAAG CAGCATGCAA GGTTGGCGAG TTGAAATGGA GGACGCACAC 
ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAGACATGGT CATTCTTTGC TGTATATGAT 
GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ACTTGTTAGA TCACATCACC 
AATAACCAGG ATTTCAGAGG ATCTGCAGGA GCACCTTCTG TGGAGAACGT AAAGAATGGA 
ATCAGAACAG GGTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 
GGTGCAGATA GAAGCGGGTC AACAGCTGTG GGCGTCTTAA TCTCTCCCCA ACATACTTAT 
TTCATTAACT GTGGAGACTC GAGAGGTTTA CTTTGTAGGA ATAGAAAAGT TCACTTCTTC 
ACACAAGACC ATAAACCAAG TAACCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGGGGC 
TCGGTGATGA TTCAGCGTGT CAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTC 
GATTACAAAT GTGTCCATGG AAAAGGTCCC ACAGAGCAGC TTGTCTCCCC AGAGCCCGAA 
GTCCATGATA TTGAAAGGTC TGAAGAAGAT GACCAGTTCA TCATCCTTGC ATGCGATGGC 
ATCTGGGACG TCATGGGGAA CGAAGAGCTC TGTGACTTTG TGAGATCCAG ACTTGAAGTC 
ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGCTTGTA TAAGGGAAGT 
CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAGTG CACCCAAAGT CTCGGCAGAG 
GCGGTGAAGA AGGAGGCGGA GCTGGACAAG TACCTGGAGA GCAGAGTAGA AGAAATCATA 
AAGAAGCAGG TGGAAGGCGT CCCTGACTTA GTCCACGTGA TGCGCACGTT AGCCAGTGAG 
AACATCCCCA GCCTCCCACC AGGGGGTGAA TTGGCAAGCA AGCGGAATGT AATTGAAGCC 
GTTTACAATA GACTGAACCC TTACAAAAAT GACGACACTG ATTCTGCGTC AACCGATGAT 
ATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1a ( XM_006515603.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515666.1 Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03069
Clone ID Related Accession (Same CDS sequence)	XM_006515603.2 , XM_006515603.3 , NM_008910.3
Accession Version	XM_006515603.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	protein phosphatase 1A isoform X1
Comment

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAGAAGCATA ATGCCCAGGG GCAGGGGAAT 
GGGTTACGAT ACGGCCTAAG CAGCATGCAA GGTTGGCGAG TTGAAATGGA GGACGCACAC 
ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAGACATGGT CATTCTTTGC TGTATATGAT 
GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ACTTGTTAGA TCACATCACC 
AATAACCAGG ATTTCAGAGG ATCTGCAGGA GCACCTTCTG TGGAGAACGT AAAGAATGGA 
ATCAGAACAG GGTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 
GGTGCAGATA GAAGCGGGTC AACAGCTGTG GGCGTCTTAA TCTCTCCCCA ACATACTTAT 
TTCATTAACT GTGGAGACTC GAGAGGTTTA CTTTGTAGGA ATAGAAAAGT TCACTTCTTC 
ACACAAGACC ATAAACCAAG TAACCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGGGGC 
TCGGTGATGA TTCAGCGTGT CAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTC 
GATTACAAAT GTGTCCATGG AAAAGGTCCC ACAGAGCAGC TTGTCTCCCC AGAGCCCGAA 
GTCCATGATA TTGAAAGGTC TGAAGAAGAT GACCAGTTCA TCATCCTTGC ATGCGATGGC 
ATCTGGGACG TCATGGGGAA CGAAGAGCTC TGTGACTTTG TGAGATCCAG ACTTGAAGTC 
ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGCTTGTA TAAGGGAAGT 
CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAGTG CACCCAAAGT CTCGGCAGAG 
GCGGTGAAGA AGGAGGCGGA GCTGGACAAG TACCTGGAGA GCAGAGTAGA AGAAATCATA 
AAGAAGCAGG TGGAAGGCGT CCCTGACTTA GTCCACGTGA TGCGCACGTT AGCCAGTGAG 
AACATCCCCA GCCTCCCACC AGGGGGTGAA TTGGCAAGCA AGCGGAATGT AATTGAAGCC 
GTTTACAATA GACTGAACCC TTACAAAAAT GACGACACTG ATTCTGCGTC AACCGATGAT 
ATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515666.1
Translation	MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSASTDDMW

Target ORF information:

RefSeq Version	XM_006515603.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515603.2

ORF Insert Sequence:

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAGAAGCATA ATGCCCAGGG GCAGGGGAAT 
GGGTTACGAT ACGGCCTAAG CAGCATGCAA GGTTGGCGAG TTGAAATGGA GGACGCACAC 
ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAGACATGGT CATTCTTTGC TGTATATGAT 
GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ACTTGTTAGA TCACATCACC 
AATAACCAGG ATTTCAGAGG ATCTGCAGGA GCACCTTCTG TGGAGAACGT AAAGAATGGA 
ATCAGAACAG GGTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 
GGTGCAGATA GAAGCGGGTC AACAGCTGTG GGCGTCTTAA TCTCTCCCCA ACATACTTAT 
TTCATTAACT GTGGAGACTC GAGAGGTTTA CTTTGTAGGA ATAGAAAAGT TCACTTCTTC 
ACACAAGACC ATAAACCAAG TAACCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGGGGC 
TCGGTGATGA TTCAGCGTGT CAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTC 
GATTACAAAT GTGTCCATGG AAAAGGTCCC ACAGAGCAGC TTGTCTCCCC AGAGCCCGAA 
GTCCATGATA TTGAAAGGTC TGAAGAAGAT GACCAGTTCA TCATCCTTGC ATGCGATGGC 
ATCTGGGACG TCATGGGGAA CGAAGAGCTC TGTGACTTTG TGAGATCCAG ACTTGAAGTC 
ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGCTTGTA TAAGGGAAGT 
CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAGTG CACCCAAAGT CTCGGCAGAG 
GCGGTGAAGA AGGAGGCGGA GCTGGACAAG TACCTGGAGA GCAGAGTAGA AGAAATCATA 
AAGAAGCAGG TGGAAGGCGT CCCTGACTTA GTCCACGTGA TGCGCACGTT AGCCAGTGAG 
AACATCCCCA GCCTCCCACC AGGGGGTGAA TTGGCAAGCA AGCGGAATGT AATTGAAGCC 
GTTTACAATA GACTGAACCC TTACAAAAAT GACGACACTG ATTCTGCGTC AACCGATGAT 
ATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1a ( XM_006515603.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515666.1 Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03069
Clone ID Related Accession (Same CDS sequence)	XM_006515603.2 , XM_006515603.3 , NM_008910.3
Accession Version	XM_006515603.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515603.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAGAAGCATA ATGCCCAGGG GCAGGGGAAT 
GGGTTACGAT ACGGCCTAAG CAGCATGCAA GGTTGGCGAG TTGAAATGGA GGACGCACAC 
ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAGACATGGT CATTCTTTGC TGTATATGAT 
GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ACTTGTTAGA TCACATCACC 
AATAACCAGG ATTTCAGAGG ATCTGCAGGA GCACCTTCTG TGGAGAACGT AAAGAATGGA 
ATCAGAACAG GGTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 
GGTGCAGATA GAAGCGGGTC AACAGCTGTG GGCGTCTTAA TCTCTCCCCA ACATACTTAT 
TTCATTAACT GTGGAGACTC GAGAGGTTTA CTTTGTAGGA ATAGAAAAGT TCACTTCTTC 
ACACAAGACC ATAAACCAAG TAACCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGGGGC 
TCGGTGATGA TTCAGCGTGT CAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTC 
GATTACAAAT GTGTCCATGG AAAAGGTCCC ACAGAGCAGC TTGTCTCCCC AGAGCCCGAA 
GTCCATGATA TTGAAAGGTC TGAAGAAGAT GACCAGTTCA TCATCCTTGC ATGCGATGGC 
ATCTGGGACG TCATGGGGAA CGAAGAGCTC TGTGACTTTG TGAGATCCAG ACTTGAAGTC 
ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGCTTGTA TAAGGGAAGT 
CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAGTG CACCCAAAGT CTCGGCAGAG 
GCGGTGAAGA AGGAGGCGGA GCTGGACAAG TACCTGGAGA GCAGAGTAGA AGAAATCATA 
AAGAAGCAGG TGGAAGGCGT CCCTGACTTA GTCCACGTGA TGCGCACGTT AGCCAGTGAG 
AACATCCCCA GCCTCCCACC AGGGGGTGAA TTGGCAAGCA AGCGGAATGT AATTGAAGCC 
GTTTACAATA GACTGAACCC TTACAAAAAT GACGACACTG ATTCTGCGTC AACCGATGAT 
ATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515666.1
CDS	74..1222
Translation	MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSASTDDMW

Target ORF information:

RefSeq Version	XM_006515603.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515603.3

ORF Insert Sequence:

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAGAAGCATA ATGCCCAGGG GCAGGGGAAT 
GGGTTACGAT ACGGCCTAAG CAGCATGCAA GGTTGGCGAG TTGAAATGGA GGACGCACAC 
ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAGACATGGT CATTCTTTGC TGTATATGAT 
GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ACTTGTTAGA TCACATCACC 
AATAACCAGG ATTTCAGAGG ATCTGCAGGA GCACCTTCTG TGGAGAACGT AAAGAATGGA 
ATCAGAACAG GGTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 
GGTGCAGATA GAAGCGGGTC AACAGCTGTG GGCGTCTTAA TCTCTCCCCA ACATACTTAT 
TTCATTAACT GTGGAGACTC GAGAGGTTTA CTTTGTAGGA ATAGAAAAGT TCACTTCTTC 
ACACAAGACC ATAAACCAAG TAACCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGGGGC 
TCGGTGATGA TTCAGCGTGT CAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTC 
GATTACAAAT GTGTCCATGG AAAAGGTCCC ACAGAGCAGC TTGTCTCCCC AGAGCCCGAA 
GTCCATGATA TTGAAAGGTC TGAAGAAGAT GACCAGTTCA TCATCCTTGC ATGCGATGGC 
ATCTGGGACG TCATGGGGAA CGAAGAGCTC TGTGACTTTG TGAGATCCAG ACTTGAAGTC 
ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGCTTGTA TAAGGGAAGT 
CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAGTG CACCCAAAGT CTCGGCAGAG 
GCGGTGAAGA AGGAGGCGGA GCTGGACAAG TACCTGGAGA GCAGAGTAGA AGAAATCATA 
AAGAAGCAGG TGGAAGGCGT CCCTGACTTA GTCCACGTGA TGCGCACGTT AGCCAGTGAG 
AACATCCCCA GCCTCCCACC AGGGGGTGAA TTGGCAAGCA AGCGGAATGT AATTGAAGCC 
GTTTACAATA GACTGAACCC TTACAAAAAT GACGACACTG ATTCTGCGTC AACCGATGAT 
ATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucose elicits serine/threonine kinase VRK1 to phosphorylate nuclear pregnane X receptor as a novel hepatic gluconeogenic signal.
Cellular signalling40200-209(2017 Dec)
Gotoh S,Miyauchi Y,Moore R,Negishi M

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

PPM1A silences cytosolic RNA sensing and antiviral defense through direct dephosphorylation of MAVS and TBK1.
Science advances2(7)e1501889(2016 07)
Xiang W,Zhang Q,Lin X,Wu S,Zhou Y,Meng F,Fan Y,Shen T,Xiao M,Xia Z,Zou J,Feng XH,Xu P

Foxf2 Is Required for Brain Pericyte Differentiation and Development and Maintenance of the Blood-Brain Barrier.
Developmental cell34(1)19-32(2015 Jul)
Reyahi A,Nik AM,Ghiami M,Gritli-Linde A,Pont?n F,Johansson BR,Carlsson P

PPM1A regulates antiviral signaling by antagonizing TBK1-mediated STING phosphorylation and aggregation.
PLoS pathogens11(3)e1004783(2015 Mar)
Li Z,Liu G,Sun L,Teng Y,Guo X,Jia J,Sha J,Yang X,Chen D,Sun Q