Nefm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nefm
Entrez Gene ID	18040
Full Name	neurofilament, medium polypeptide
Synonyms	NF-M,NF160,NF165,Nef3,Nfm
General protein information	Preferred Names neurofilament, medium polypeptide Names neurofilament medium polypeptide 160 kDa neurofilament protein neurofilament 3, medium neurofilament protein M neurofilament triplet M protein neurofilament-M
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_008691.2	NP_032717.2	neurofilament medium polypeptide

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nefm	NP_032717.2
Rattus norvegicus (Norway rat)	Nefm	NP_058725.1

Gene Ontology

The following Nefm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nefm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18645	NM_008691.2 Latest version!	Mus musculus neurofilament, medium polypeptide (Nefm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nefm ( NM_008691.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032717.2 Mus musculus neurofilament, medium polypeptide (Nefm), mRNA.

Price & Availability↑

CloneID	OMu18645
Clone ID Related Accession (Same CDS sequence)	NM_008691.2
Accession Version	NM_008691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	neurofilament medium polypeptide
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE986344.1, AK051696.1, AC154687.2 and BF466963.1. On Aug 16, 2006 this sequence version replaced NM_008691.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058333.1, AK051696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849378, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTACA CGCTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT CACCGAGACC 
CGCTCCAGCT TCAGCCGCGT GAGCGGTTCC CCGTCCAGCG GCTTCCGCTC GCAGTCCTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT CGCCCCGCGT 
CTCGCCTACA GCTCGGCTAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCGCTGC TCAACGGCGG CTCCGGCGGC GACTACAAAC TGTCCCGCTC TAACGAGAAA 
GAGCAGCTGC AGGGGCTGAA CGACCGCTTC GCCGGCTACA TCGAGAAAGT GCACTACTTG 
GAACAACAGA ACAAGGAGAT CGAAGCAGAG ATCCAGGCAC TGCGGCAGAA GCAGGCCTCG 
CACGCCCAGC TGGGTGATGC TTACGACCAG GAGATCCGAG AGCTGCGCGC CACCCTCGAG 
ATGGTGAACC ACGAGAAGGC TCAAGTGCAG CTGGACTCCG ATCACTTGGA GGAAGACATC 
CACCGGCTCA AGGAGCGCTT CGAGGAGGAG GCGCGGCTGC GGGACGACAC CGAGGCTGCC 
ATTCGCGCGC TGCGCAAAGA CATCGAAGAG TCGTCGATGG TTAAGGTGGA GCTGGACAAG 
AAGGTGCAGT CGCTGCAGGA TGAGGTGGCT TTCCTGCGGA GCAATCACGA AGAGGAGGTG 
GCCGACCTGC TGGCTCAGAT CCAGGCGTCG CACATCACGG TAGAGCGCAA AGATTACCTG 
AAGACAGACA TCTCCACGGC GCTGAAGGAG ATCCGCTCCC AGCTCGAGTG TCACTCAGAC 
CAGAACATGC ACCAGGCCGA AGAGTGGTTC AAATGCCGCT ACGCCAAGCT CACCGAGGCG 
GCCGAGCAGA ACAAGGAGGC CATTCGCTCT GCCAAGGAAG AGATCGCCGA GTACCGGCGC 
CAGCTGCAGT CCAAGAGCAT CGAGCTCGAG TCGGTGCGAG GCACTAAGGA GTCCCTGGAG 
CGGCAGCTCA GCGACATCGA GGAGCGCCAC AACCACGACC TCAGCAGCTA CCAGGACACC 
ATCCAGCAGT TGGAAAATGA ACTTCGGGGA ACCAAGTGGG AAATGGCTCG TCATTTGCGA 
GAATACCAGG ATCTCCTTAA CGTCAAGATG GCCCTGGACA TCGAGATCGC CGCGTACAGG 
AAACTCCTAG AGGGGGAAGA GACCAGATTT AGCACATTTT CAGGAAGCAT CACCGGGCCT 
CTGTACACAC ACCGACAGCC CTCAGTCACA ATATCCAGTA AGATTCAGAA GACCAAAGTC 
GAGGCCCCCA AGCTCAAGGT CCAACACAAA TTTGTGGAGG AGATCATCGA AGAAACTAAA 
GTGGAAGATG AGAAGTCAGA AATGGAAGAA ACCCTCACAG CCATCGCAGA GGAGTTGGCA 
GCCTCCGCCA AAGAGGAGAA GGAAGAGGCC GAAGAAAAGG AGGAGGAACC AGAAGCCGAA 
AAGTCTCCCG TGAAGTCTCC TGAGGCTAAG GAAGAGGAGG AGGAAGGGGA AAAGGAGGAA 
GAAGAGGAAG GCCAGGAGGA AGAAGAGGAG GAAGATGAAG GTGTCAAGTC AGACCAGGCA 
GAAGAGGGGG GATCTGAGAA GGAAGGCTCC AGTGAAAAAG ATGAAGGTGA GCAGGAAGAA 
GAAGAAGGAG AAACCGAGGC AGAAGGTGAA GGAGAGGAAG CAGAAGCTAA GGAGGAAAAG 
AAAATTGAGG GAAAGGTTGA GGAAGTGGCT GTCAAGGAGG AAATCAAGGT CGAGAAGCCT 
GAGAAAGCCA AATCCCCTAT GCCCAAATCA CCCGTGGAAG AAGTAAAGCC AAAACCAGAG 
GCCAAGGCCG GGAAGGGTGA GCACAAGGAG GAAGAGAAAG TTGAGGAAGA GAAGAAGGAA 
GTCACCAAAG AATCACCCAA GGAAGAGAAG GTGGAGAAAA AGGAGGAGAA GCCAAAAGAT 
GTTGCAGATA AAAAGAAGGC CGAGTCCCCG GTGAAAGAGA AGGCTGTGGA GGAGGTGATC 
ACCATCAGCA AGTCGGTAAA GGTGAGCCTA GAGAAAGACA CCAAAGAGGA GAAGCTGCAG 
CCGCAGGAGA AGGTGAAGGA GAAGGCAGAG GAGGAGGGGG GCAGTGAGGA GGAAGGGAGT 
GACCGTAGCC CGCAGGAGTC CAAGAAGGAA GACATAGCTA TCAATGGGGA GGTGGAAGGA 
AAAGAGGAGG AGGAGCAGGA AACTCAGGAG AAGGGCAGTG GGCGGGAGGA GGAGAAAGGG 
GTGGTCACTA ATGGCTTAGA TGTGAGCCCT GCAGAGGAGA AGAAAGGAGA GGATAGCAGT 
GATGATAAAG TGGTGGTCAC CAAGAAGGTA GAAAAGATCA CCAGCGAGGG GGGCGATGGT 
GCTACCAAAT ACATCACCAA ATCTGTAACC GTCACTCAAA AGGTTGAAGA GCATGAGGAG 
ACCTTTGAGG AGAAGCTGGT CTCAACTAAA AAGGTAGAAA AGGTCACTTC ACACGCCATA 
GTCAAGGAAG TCACCCAGGG TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032717.2
CDS	192..2738
Translation	MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLECHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGPLYTHRQPSVTISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEETLTAIAEELAASAKEEKEEAEEKEEEPEAEKSPVKSPEAKEEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEEEEGETEAEGEGEEAEAKEEKKIEGKVEEVAVKEEIKVEKPEKAKSPMPKSPVEEVKPKPEAKAGKGEHKEEEKVEEEKKEVTKESPKEEKVEKKEEKPKDVADKKKAESPVKEKAVEEVITISKSVKVSLEKDTKEEKLQPQEKVKEKAEEEGGSEEEGSDRSPQESKKEDIAINGEVEGKEEEEQETQEKGSGREEEKGVVTNGLDVSPAEEKKGEDSSDDKVVVTKKVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQGD

Target ORF information:

RefSeq Version	NM_008691.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofilament, medium polypeptide (Nefm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008691.2

ORF Insert Sequence:

ATGAGCTACA CGCTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT CACCGAGACC 
CGCTCCAGCT TCAGCCGCGT GAGCGGTTCC CCGTCCAGCG GCTTCCGCTC GCAGTCCTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT CGCCCCGCGT 
CTCGCCTACA GCTCGGCTAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCGCTGC TCAACGGCGG CTCCGGCGGC GACTACAAAC TGTCCCGCTC TAACGAGAAA 
GAGCAGCTGC AGGGGCTGAA CGACCGCTTC GCCGGCTACA TCGAGAAAGT GCACTACTTG 
GAACAACAGA ACAAGGAGAT CGAAGCAGAG ATCCAGGCAC TGCGGCAGAA GCAGGCCTCG 
CACGCCCAGC TGGGTGATGC TTACGACCAG GAGATCCGAG AGCTGCGCGC CACCCTCGAG 
ATGGTGAACC ACGAGAAGGC TCAAGTGCAG CTGGACTCCG ATCACTTGGA GGAAGACATC 
CACCGGCTCA AGGAGCGCTT CGAGGAGGAG GCGCGGCTGC GGGACGACAC CGAGGCTGCC 
ATTCGCGCGC TGCGCAAAGA CATCGAAGAG TCGTCGATGG TTAAGGTGGA GCTGGACAAG 
AAGGTGCAGT CGCTGCAGGA TGAGGTGGCT TTCCTGCGGA GCAATCACGA AGAGGAGGTG 
GCCGACCTGC TGGCTCAGAT CCAGGCGTCG CACATCACGG TAGAGCGCAA AGATTACCTG 
AAGACAGACA TCTCCACGGC GCTGAAGGAG ATCCGCTCCC AGCTCGAGTG TCACTCAGAC 
CAGAACATGC ACCAGGCCGA AGAGTGGTTC AAATGCCGCT ACGCCAAGCT CACCGAGGCG 
GCCGAGCAGA ACAAGGAGGC CATTCGCTCT GCCAAGGAAG AGATCGCCGA GTACCGGCGC 
CAGCTGCAGT CCAAGAGCAT CGAGCTCGAG TCGGTGCGAG GCACTAAGGA GTCCCTGGAG 
CGGCAGCTCA GCGACATCGA GGAGCGCCAC AACCACGACC TCAGCAGCTA CCAGGACACC 
ATCCAGCAGT TGGAAAATGA ACTTCGGGGA ACCAAGTGGG AAATGGCTCG TCATTTGCGA 
GAATACCAGG ATCTCCTTAA CGTCAAGATG GCCCTGGACA TCGAGATCGC CGCGTACAGG 
AAACTCCTAG AGGGGGAAGA GACCAGATTT AGCACATTTT CAGGAAGCAT CACCGGGCCT 
CTGTACACAC ACCGACAGCC CTCAGTCACA ATATCCAGTA AGATTCAGAA GACCAAAGTC 
GAGGCCCCCA AGCTCAAGGT CCAACACAAA TTTGTGGAGG AGATCATCGA AGAAACTAAA 
GTGGAAGATG AGAAGTCAGA AATGGAAGAA ACCCTCACAG CCATCGCAGA GGAGTTGGCA 
GCCTCCGCCA AAGAGGAGAA GGAAGAGGCC GAAGAAAAGG AGGAGGAACC AGAAGCCGAA 
AAGTCTCCCG TGAAGTCTCC TGAGGCTAAG GAAGAGGAGG AGGAAGGGGA AAAGGAGGAA 
GAAGAGGAAG GCCAGGAGGA AGAAGAGGAG GAAGATGAAG GTGTCAAGTC AGACCAGGCA 
GAAGAGGGGG GATCTGAGAA GGAAGGCTCC AGTGAAAAAG ATGAAGGTGA GCAGGAAGAA 
GAAGAAGGAG AAACCGAGGC AGAAGGTGAA GGAGAGGAAG CAGAAGCTAA GGAGGAAAAG 
AAAATTGAGG GAAAGGTTGA GGAAGTGGCT GTCAAGGAGG AAATCAAGGT CGAGAAGCCT 
GAGAAAGCCA AATCCCCTAT GCCCAAATCA CCCGTGGAAG AAGTAAAGCC AAAACCAGAG 
GCCAAGGCCG GGAAGGGTGA GCACAAGGAG GAAGAGAAAG TTGAGGAAGA GAAGAAGGAA 
GTCACCAAAG AATCACCCAA GGAAGAGAAG GTGGAGAAAA AGGAGGAGAA GCCAAAAGAT 
GTTGCAGATA AAAAGAAGGC CGAGTCCCCG GTGAAAGAGA AGGCTGTGGA GGAGGTGATC 
ACCATCAGCA AGTCGGTAAA GGTGAGCCTA GAGAAAGACA CCAAAGAGGA GAAGCTGCAG 
CCGCAGGAGA AGGTGAAGGA GAAGGCAGAG GAGGAGGGGG GCAGTGAGGA GGAAGGGAGT 
GACCGTAGCC CGCAGGAGTC CAAGAAGGAA GACATAGCTA TCAATGGGGA GGTGGAAGGA 
AAAGAGGAGG AGGAGCAGGA AACTCAGGAG AAGGGCAGTG GGCGGGAGGA GGAGAAAGGG 
GTGGTCACTA ATGGCTTAGA TGTGAGCCCT GCAGAGGAGA AGAAAGGAGA GGATAGCAGT 
GATGATAAAG TGGTGGTCAC CAAGAAGGTA GAAAAGATCA CCAGCGAGGG GGGCGATGGT 
GCTACCAAAT ACATCACCAA ATCTGTAACC GTCACTCAAA AGGTTGAAGA GCATGAGGAG 
ACCTTTGAGG AGAAGCTGGT CTCAACTAAA AAGGTAGAAA AGGTCACTTC ACACGCCATA 
GTCAAGGAAG TCACCCAGGG TGACTAA