Myo7b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo7b
Entrez Gene ID	17922
Full Name	myosin VIIB
General protein information	Preferred Names myosin VIIB Names unconventional myosin-VIIb myosin-VIIb
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 B1\|18 17.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_032394.3	NP_115770.2	unconventional myosin-VIIb

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Myo7b	NP_001178870.1
Gallus gallus (chicken)	MYO7B	XP_003641791.1
	MYO7B	XP_002799468.1
Bos taurus (cattle)	MYO7B	XP_002685259.2
Xenopus tropicalis (tropical clawed frog)	myo7b	XP_002936659.2
Mus musculus (house mouse)	Myo7b	NP_115770.2
Homo sapiens (human)	MYO7B	NP_001073996.1
Pan troglodytes (chimpanzee)	MYO7B	XP_515778.3
Canis lupus familiaris (dog)	MYO7B	XP_005631987.1
Caenorhabditis elegans (roundworm)	hum-6	NP_508420.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Intestinal brush border assembly driven by protocadherin-based intermicrovillar adhesion.
Crawley SW, Shifrin DA Jr, Grega-Larson NE, McConnell RE, Benesh AE, Mao S, Zheng Y, Zheng QY, Nam KT, Millis BA, Kachar B, Tyska MJ
Cell157(2)433-446(2014 Apr)

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Revenu C, Ubelmann F, Hurbain I, El-Marjou F, Dingli F, Loew D, Delacour D, Gilet J, Brot-Laroche E, Rivero F, Louvard D, Robine S
Molecular biology of the cell23(2)324-36(2012 Jan)

Vertebrate myosin VIIb is a high duty ratio motor adapted for generating and maintaining tension.
Henn A, De La Cruz EM
The Journal of biological chemistry280(47)39665-76(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cloned, expressed and purified the catalytic motor domain, and characterized its actin-activated ATPase cycle
Title: Vertebrate myosin VIIb is a high duty ratio motor adapted for generating and maintaining tension.

The following Myo7b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo7b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08674	NM_032394.3 Latest version!	Mus musculus myosin VIIB (Myo7b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo7b ( NM_032394.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_115770.2 Mus musculus myosin VIIB (Myo7b), mRNA.

Price & Availability↑

CloneID	OMu08674
Clone ID Related Accession (Same CDS sequence)	NM_032394.3
Accession Version	NM_032394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIb
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC138341.1, AK220152.1 and AC131761.4. On Oct 21, 2009 this sequence version replaced NM_032394.2. ##Evidence-Data-START## Transcript exon combination :: AF242411.1, BC138338.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGTGT TCCGGCTGGG TGACCATGTG TGGCTGGACC CTCCCTCCAG CAGCAAGACT 
GGTGTGGCCA TTGGGGGCAT TGTCAAAGAA ACAAAACTGG GAAAGACCTT GATTGAGGAT 
GACGAGGGCA AGGAACACTG GGTCCATGCA GAAGACCTCA GCACCCTCAG GCCTATGCAC 
CCCAACTCAG CCCAAGGAGT GGATGACATG ATCCGCCTAG GGGACCTGAA TGAGGCTGGT 
GTGGTGCACA ACCTCCTGAT CCGGTACCAG CAGCACAAGA TCTATACCTA CACAGGCTCC 
ATCCTGGTGG CTGTGAACCC ATTCCAGATG CTGCCCCTGT ACACCCTGGA GCAGGTACAA 
ATCTACTACA GCCGCCACAT GGGCGAGCTG CCTCCCCACA TCTTCGCCAT TGCCAACAGC 
TGCTACTTCA ACATGAAGAA GAACAAGAGG GACCAGTGCT GTATCATCAG TGGCGAGTCT 
GGGGCCGGGA AGACCGAGAC CACCAAGCTT ATCCTTCAGT TCCTCGCCAC GGTCAGTGGC 
CAGCATTCGT GGATCGAGCA ACAGGTCCTG GAGGCCAACC CCATCCTGGA AGCCTTTGGC 
AATGCCAAGA CCATCCGCAA TGACAACTCC AGCCGCTTCG GGAAGTACAT TGACATCCAC 
TTCAATTCTA GTGGGGTGAT TGAGGGCGCA AGCATTGAAC ACTTCCTCCT GGAGAAGTCC 
AGAGTCTGCC GGCAGGCTCC AGAGGAACGC AACTACCACA TCTTCTACTG CATGCTGATG 
GGGATGAGCC CGGAAGAGAA GCAAATGCTG AGTCTGGGCA TGCCCTCTGA GTACCATTAC 
CTGACCATGG GGAGCTGCAC ATCCTCCGAG GGGCTCAGCG ATGCCAAGGA CTACGCACAC 
GTCCGCTCGG CCATGAAGAT CCTCCAGTTC TCAGACTCAG AGAACTGGGA CATCAGCAAG 
CTGCTGGCAG CCATCCTCCA CCTGGGGAAT GTGGGGTTCA TGGCTGCAGT CTTTGAGAAC 
CTGGATTCGT CTGATGTGAT GGAAACTCCA GCCTTCCCCT TGGCAATGAA GTTGCTAGAG 
GTGCAGCACC AGGCTCTCCG AGACTGTTTG ATCAAACACA CAATCCCTGT CCTTGGAGAG 
TTTGTCTCCA GGCCTGTGAA CATCGCCCAG GCTACAGACC GGAGGGATGC CTTTGTCAAG 
GGCATCTATG GGCGCCTCTT CCAGTGGATT GTCAAGAAAA TCAATGCTGC GATCTTCACT 
CCCCAAGCCC AGGATCCCCA AAATGTGCGG AGGGCCATCG GTCTCCTGGA CATATTTGGC 
TTTGAAAACT TCCAGAACAA TAGCTTTGAG CAACTCTGCA TCAACTTTGC CAACGAGCAC 
CTTCAGCAAT TCTTCGTGAA GCACGTGTTC ACGATGGAGC AGGAAGAGTA CCTGTCTGAG 
AACATCACCT GGAACTACAT CCACTACACC GACAACCAGC CCATCCTGGA CATGCTGGCC 
CTCAAGCCCA TGAGCATCAT CTCTCTCCTA GATGAGGAGA GCCGCTTCCC ACAGGGGACA 
GATGTCACTA TGCTGCAGAA ACTGAACAGT ATCCATGCCA ACAACAAGTC CTTCCTGAGC 
CCCAGGAGCA TCCATGACAC CCGATTTGGG ATCGCACACT TCGCGGGCGA TGTATACTAC 
CAGGCAGAAG GCTTTCTGGA GAAGAATCGA GATGTGCTGA GCACAGATAT CCTAATCCTG 
ATTCATTCCT CGAAAAACAA GTTTCTGAAG GAGATATTTA ACGTGGACTC ATCACAGACT 
AAGCTGGGGC ACGGCACCAT CTGCCAGGTG AAGGCAGGAA GCCAGCTCTT CAAGTCATCA 
GACTCCATCA AGCGCCCCGT CACCCTCGCG AGCCAGTTCA AGCAGTCTCT GGACCAGCTG 
ATGAGAATCC TGACCAACTG CCAGCCCTAT TTTGTCCGCT GCATTAAACC CAATGAGTAC 
AAGAAGCCAC TGCTGTTTGA CCGAGAGCTG TGCATTCAGC AGCTGCGTTA TTCCGGCATG 
ATGGAGACTG TGCACATCCG CAAGTCAGGT TTTCCCATCC GCTACACATT TGATGAGTTC 
TCACAGAGAT TCCGTGTGCT GCTGCCCAGC CCGGAGCGTA TGCAGTTTCA GAACAAGCCC 
CGCCAGATGA CCTTGCACAT TGCTGACCTA TGCCTGGGGA CAGACAAAGA CTGGAAAGTC 
GGAAAGACCA AAATCTTCCT GAAGGATCAT CAGGACACAG TGCTGGAGAT CCGCAGGAGC 
CAGGCCCTGG ACGGAGCAGC GATCCGGATC CAGAGAGTCC TCCGGGGACA CAAGTACAGG 
AAGGAGTTCC TGAGGCAGAG ACGTGCAGCT GTGACCCTCC AGGCAGGGTG GCGAGGCTAT 
AGCCAAAGAA AGAATTTCAA GCTGATCCTA GTGGGCTTTG AACGCCTGCA GGCCATCGCC 
CGGAGCCACC TGCTGATGAG ACAGTTCCAG GCCATGCGAC AGAGGATAGT GCAGCTGCAG 
GCCCGATGCA GGGGCTACCT GGTGCGACAG CAAGTCCAGG CCAAGAGGAG GGCAGTGGTG 
ATCATTCAAG CACATGCCAG GGGCATGGTT GTCCGGAAGA GCTACTGGCA ACAGAAAAGC 
ACTGGGCCTC AGGTCATTCT GGCTAAGGAA CCAAAGGCTC AAGTTGCGGT CCATGAGAGG 
AAGCGTAAGT CCATCTATGA CACTGTCACT GACACAGCGA TGGTGGAGAA AGTGTTTGGC 
TTCCTCCCAG CCATGATTGG AGGTCAGGAG GGTCCAGCCC CAACACGCTT TGAGGATCTG 
GAAGTAAAGA CCCAGAAACT TCATGAGGTT GATCTGGATA CAGTGCCCAT GATGGCGATG 
CCAGAAGAGG AGGTGGACAG CCTGGCTGAG TATACCTTCC CCAAGTTTGC TGTGACTTAC 
TTCCAGAAGT CAGCCAGCCA CACCCACATC CAGAAGCCCC TTCGATACCC ACTCCTCTAC 
CATGAAAATG ACACTGACCA CTCGGCTGCT CTAGATGTTT GGATTATCAT CCTGAGGTTC 
ATGGGTGACC TTCCAGAGCC AGTGGTCTAT GGCAGGAACA GCCTGACTGG CAGCTCAGTG 
ATGCGGCAGA TCCATGACAA ACTGGGCAAG GACAGTGTCA CTCAGCACAA CAGATCCTCA 
CAGGTGGCCA GCCAGCTGAA CTTTGGTGAA GAGGCATTCA AGTTTGATGG CCCTATCTCA 
GACAGACCCA TGTCTAACCT AGAGAAGGTG CACTTCATTG TGGGCTACGC CATCATGCGT 
CCTGGACTCA GAGATGAAAT CTACTGTCAG ATCTGCAAGC AGCTCTCCGA GAACTACAAA 
ACAAGTAGCC GGGCCCGGGG CTGGATCCTG CTCAGCCTCT GTCTGGGATG CTTCCCACCC 
TCAGAGAGGT TCATGAAGTA TCTGCTGAAT TTCATTAGTC AAGGGCCACC CAGCTATGGG 
CCCTTCTGTG CTGAACGGCT GCAACGCACC TTCGCCAATG GGGTGCGCGC AGAACCCCCC 
ACCTGGCTGG AGCTGCAGGC TGTCAAGTCC AAGAAGCACA TCCCGATCCA GGTCATCCTG 
GCAACCGGAA GGAGCCTGAC CATCTCTGTG GACTCCGCCT CCACATCACG AGAAATCTGC 
CAGCACGTCG CTCAGAAACA GGGTCTCAGG GACAATCTGG GCTTCTCCCT CCAGGTCGCT 
GTATATGATA AGTTCTGGTC CTTGGGCAGC GGCTGTGACC ATTTGATGGA TGCTGTGGCC 
CAGTGTGAGC AACTGGCCCG AGAGAGGGGA GAGAGCCAGC GGCAGGCCCC CTGGCGTATC 
TACTTCCGGA AGGAGTTCTT CACCCCCTGG CATGACTCCC AGGAGGACCC TGTGAGCACT 
GAGCTCATTT ACCACCAAGT CCTCCGTGGG GTGTGGTCTG GCGAGTATAA CTTTGAGAAG 
GAGGAGGAGC TTGTGGAGCT GCTGGCCCGG CACTGCTATG TGCAGCTTGG GGCCACGGTG 
AAGAGTAACG CTGTCCAGGA GCTGTTGCCC AGCTGTGTTC CCTCCAAACT GTACCGGACC 
AAGTCTCCAG AGAAGTGGGC TAGCCTTGTC ACCGCTGCCC ATGCCAAGGC CCAGTATACC 
CAGTCGAAAG CCACGCCACT GGCGGTGAGG GAGCAGACAG TGGAGGCTGC CCGTCTTCTG 
TGGCCCCTGC TCTTCTCCCG GCTATTTGAA GTCACCACAC TCTCTGGTCC CCGCCTTCCC 
AAGACGCAGC TGGTTTTAGC CATTAACTGG AAGGGGATGT ACTTCCTGGA TCAGAAGGAG 
AGGACACTGC TGGGGTTGTC CTTCGCAGAG GTCATGGGTC TGGTCGCCAA CAGGGACGCC 
CCAGGAGGGA AGAAGCTGCT TCTCGCCACA CTCCAGGAAG AGTACGAGTT CGTCTCCCCC 
AGCAGCGTGG CTATTGCAGA GATGGTGGCC TTGTTCTTGG GAGGCTTGAA GGAGAGATCT 
GTGTTCGCCA TGGCCCTGCA GGACAGAAGG GCCACAGACG ATATCACCCT TCTGCCCTTC 
AAGAAAGGTG ACTTACTGAT CCTGACCAAG AAACAAGGAC TGTTGGCCTC AGAGAACTGG 
GCTCTGGGTC AGAATGACAG AACTGGCAAG ACAGGGCTGG TGCCTACTGC CTGTCTCTAC 
ACCATCCCCT CGGTCACCAA GCCCTCAACC CAGCTGCTGA GCTTGCTGGC CATGTCACCA 
GAGAAACGGA AGCTGGCAGC CCAGGAGGTG CGGGCTCTGG AGCCACCACT GGAGGATCAG 
CTCACAGAGT CGCCATACAC CCTGGAGGAA TTCTCCTACC AGTTCTTCAG GGCTCCGGAG 
AAAGAGACTA TCAGCAGGGC TGCGATGCCC ATGGCCCGAA GCCGGGGTCA TCTCTGGGCC 
TACTCCCCAG AGCCGCTGCG CCAGCCACTG CTCAAATCTG TCCACGACAA AGCCAAACTG 
CGGGATGCAG CCTGCCAGAT CTTCCTTGCC ATCCTCAAGT ACACAGGTGA CTACCCTTCC 
CGCCAGTCCT GGCACAGCCT GGAGCTCACA GACCAGATGT TCTCGTTAGC CCTGCAGGAC 
CCAGCCCTGC AGGATGAGCT GTACTGCCAG ATCCTGAAGC AGCTGACCCA CAACTCCATC 
AGGTTCAGTG AAGAGCGAGC TTGGCAGCTA CTCTGGCTCT GCACAGGTCT CTTCCCACCT 
GGGAAGACAT TGCTGCCCCA CGCTCAGAAG TTCATAGACT CCCGGAAGAA GAAGCCGCTG 
GCTCTCGACT GTAGCCGCAG GCTCCACAGG GTTCTAAGGG TGGGACCTCG GAAGCAGCCC 
CCACATGATG TAGAGGTGAA AGCGGCTGAG CAGAATGTCT CCAAATTACA CCATGAGGTC 
TACCTCCCCA ATGACACCAG CAAGTCCATG GAAGTGGGCA GCAGTAGTCG GGTGAGAGAC 
CTATGTGAGG GCATAGGCAC CAGGCTCCAG CTGGCCTCCT GGGATGGCTG CAGCCTCTTC 
ATCAAGATCA CAGACAAGGT CATCAGTCTG AAGGAAGGAG ACTTCTTTTT TGACAGCTTG 
AGGCAGGTAT CTGACTGGGT GAAGAAGAAC AGGCCCCAGA AAGAAGGGGC CTCCGTGACA 
CTTCCCTATC AGGTGTTCTT CATGCGGAAA CTGTGGCTCA ACGTGACCCC TGGGAAGGAC 
GTGAATGCAG ACACCATACT CCATTACCAC CAGGAACTAC CCAAGTACCT CCGCGGGTTT 
CACAAGTGCT CCCGGGAAGA TGCCATCCAC CTGGGAGGTC TCATCTGTAA AATCCAGTTT 
GGTAGTGACA GCTCGCAACT GGCTAGTGTC TCAAAGGTCC TGAAGGAGCT GGTACCTCAG 
AACCTCACAC GCCTGATGTC CTCAGAAGAA TGGAAAAAGA GCCTCCTTCT GGAATGTGAC 
AAAAACAAGC GCAAGACAGT GGCAGAGGCC AAAGTGGAGT TCTTGAAGTA CATGTACAGG 
TGGCCCACCT TTGGGTCTGC CTTCTTTGAG GTGAAGCAAA CCTCTGAGCC TTCCTACCCA 
GACATTCTCC TCATCGCCAT CAACCGACAT GGGTTACTGC TCATCCACCC CAAGACCAAG 
GAGCTGCTGA ACACCTACCC TTTCACCAAG ATCTCCAGCT GGAGTAGTGG CAACACCTAT 
TTCCACATGG CACTGGGGAG CCTGGGCCAG GGCAGCCGTT TGCTGTGTGA GACCTCTCTG 
GGTTACAAGA TGGACGACCT GCTGACCTCA TATGTACAGC AGCTCCTGAA TACAGTGAAC 
AAACAGCGGG GCTTCAGGGC TCCCGCCCCG GCCAACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115770.2
CDS	83..6424
Translation	MSVFRLGDHVWLDPPSSSKTGVAIGGIVKETKLGKTLIEDDEGKEHWVHAEDLSTLRPMHPNSAQGVDDMIRLGDLNEAGVVHNLLIRYQQHKIYTYTGSILVAVNPFQMLPLYTLEQVQIYYSRHMGELPPHIFAIANSCYFNMKKNKRDQCCIISGESGAGKTETTKLILQFLATVSGQHSWIEQQVLEANPILEAFGNAKTIRNDNSSRFGKYIDIHFNSSGVIEGASIEHFLLEKSRVCRQAPEERNYHIFYCMLMGMSPEEKQMLSLGMPSEYHYLTMGSCTSSEGLSDAKDYAHVRSAMKILQFSDSENWDISKLLAAILHLGNVGFMAAVFENLDSSDVMETPAFPLAMKLLEVQHQALRDCLIKHTIPVLGEFVSRPVNIAQATDRRDAFVKGIYGRLFQWIVKKINAAIFTPQAQDPQNVRRAIGLLDIFGFENFQNNSFEQLCINFANEHLQQFFVKHVFTMEQEEYLSENITWNYIHYTDNQPILDMLALKPMSIISLLDEESRFPQGTDVTMLQKLNSIHANNKSFLSPRSIHDTRFGIAHFAGDVYYQAEGFLEKNRDVLSTDILILIHSSKNKFLKEIFNVDSSQTKLGHGTICQVKAGSQLFKSSDSIKRPVTLASQFKQSLDQLMRILTNCQPYFVRCIKPNEYKKPLLFDRELCIQQLRYSGMMETVHIRKSGFPIRYTFDEFSQRFRVLLPSPERMQFQNKPRQMTLHIADLCLGTDKDWKVGKTKIFLKDHQDTVLEIRRSQALDGAAIRIQRVLRGHKYRKEFLRQRRAAVTLQAGWRGYSQRKNFKLILVGFERLQAIARSHLLMRQFQAMRQRIVQLQARCRGYLVRQQVQAKRRAVVIIQAHARGMVVRKSYWQQKSTGPQVILAKEPKAQVAVHERKRKSIYDTVTDTAMVEKVFGFLPAMIGGQEGPAPTRFEDLEVKTQKLHEVDLDTVPMMAMPEEEVDSLAEYTFPKFAVTYFQKSASHTHIQKPLRYPLLYHENDTDHSAALDVWIIILRFMGDLPEPVVYGRNSLTGSSVMRQIHDKLGKDSVTQHNRSSQVASQLNFGEEAFKFDGPISDRPMSNLEKVHFIVGYAIMRPGLRDEIYCQICKQLSENYKTSSRARGWILLSLCLGCFPPSERFMKYLLNFISQGPPSYGPFCAERLQRTFANGVRAEPPTWLELQAVKSKKHIPIQVILATGRSLTISVDSASTSREICQHVAQKQGLRDNLGFSLQVAVYDKFWSLGSGCDHLMDAVAQCEQLARERGESQRQAPWRIYFRKEFFTPWHDSQEDPVSTELIYHQVLRGVWSGEYNFEKEEELVELLARHCYVQLGATVKSNAVQELLPSCVPSKLYRTKSPEKWASLVTAAHAKAQYTQSKATPLAVREQTVEAARLLWPLLFSRLFEVTTLSGPRLPKTQLVLAINWKGMYFLDQKERTLLGLSFAEVMGLVANRDAPGGKKLLLATLQEEYEFVSPSSVAIAEMVALFLGGLKERSVFAMALQDRRATDDITLLPFKKGDLLILTKKQGLLASENWALGQNDRTGKTGLVPTACLYTIPSVTKPSTQLLSLLAMSPEKRKLAAQEVRALEPPLEDQLTESPYTLEEFSYQFFRAPEKETISRAAMPMARSRGHLWAYSPEPLRQPLLKSVHDKAKLRDAACQIFLAILKYTGDYPSRQSWHSLELTDQMFSLALQDPALQDELYCQILKQLTHNSIRFSEERAWQLLWLCTGLFPPGKTLLPHAQKFIDSRKKKPLALDCSRRLHRVLRVGPRKQPPHDVEVKAAEQNVSKLHHEVYLPNDTSKSMEVGSSSRVRDLCEGIGTRLQLASWDGCSLFIKITDKVISLKEGDFFFDSLRQVSDWVKKNRPQKEGASVTLPYQVFFMRKLWLNVTPGKDVNADTILHYHQELPKYLRGFHKCSREDAIHLGGLICKIQFGSDSSQLASVSKVLKELVPQNLTRLMSSEEWKKSLLLECDKNKRKTVAEAKVEFLKYMYRWPTFGSAFFEVKQTSEPSYPDILLIAINRHGLLLIHPKTKELLNTYPFTKISSWSSGNTYFHMALGSLGQGSRLLCETSLGYKMDDLLTSYVQQLLNTVNKQRGFRAPAPANP

Target ORF information:

RefSeq Version	NM_032394.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIB (Myo7b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032394.3

ORF Insert Sequence:

ATGTCCGTGT TCCGGCTGGG TGACCATGTG TGGCTGGACC CTCCCTCCAG CAGCAAGACT 
GGTGTGGCCA TTGGGGGCAT TGTCAAAGAA ACAAAACTGG GAAAGACCTT GATTGAGGAT 
GACGAGGGCA AGGAACACTG GGTCCATGCA GAAGACCTCA GCACCCTCAG GCCTATGCAC 
CCCAACTCAG CCCAAGGAGT GGATGACATG ATCCGCCTAG GGGACCTGAA TGAGGCTGGT 
GTGGTGCACA ACCTCCTGAT CCGGTACCAG CAGCACAAGA TCTATACCTA CACAGGCTCC 
ATCCTGGTGG CTGTGAACCC ATTCCAGATG CTGCCCCTGT ACACCCTGGA GCAGGTACAA 
ATCTACTACA GCCGCCACAT GGGCGAGCTG CCTCCCCACA TCTTCGCCAT TGCCAACAGC 
TGCTACTTCA ACATGAAGAA GAACAAGAGG GACCAGTGCT GTATCATCAG TGGCGAGTCT 
GGGGCCGGGA AGACCGAGAC CACCAAGCTT ATCCTTCAGT TCCTCGCCAC GGTCAGTGGC 
CAGCATTCGT GGATCGAGCA ACAGGTCCTG GAGGCCAACC CCATCCTGGA AGCCTTTGGC 
AATGCCAAGA CCATCCGCAA TGACAACTCC AGCCGCTTCG GGAAGTACAT TGACATCCAC 
TTCAATTCTA GTGGGGTGAT TGAGGGCGCA AGCATTGAAC ACTTCCTCCT GGAGAAGTCC 
AGAGTCTGCC GGCAGGCTCC AGAGGAACGC AACTACCACA TCTTCTACTG CATGCTGATG 
GGGATGAGCC CGGAAGAGAA GCAAATGCTG AGTCTGGGCA TGCCCTCTGA GTACCATTAC 
CTGACCATGG GGAGCTGCAC ATCCTCCGAG GGGCTCAGCG ATGCCAAGGA CTACGCACAC 
GTCCGCTCGG CCATGAAGAT CCTCCAGTTC TCAGACTCAG AGAACTGGGA CATCAGCAAG 
CTGCTGGCAG CCATCCTCCA CCTGGGGAAT GTGGGGTTCA TGGCTGCAGT CTTTGAGAAC 
CTGGATTCGT CTGATGTGAT GGAAACTCCA GCCTTCCCCT TGGCAATGAA GTTGCTAGAG 
GTGCAGCACC AGGCTCTCCG AGACTGTTTG ATCAAACACA CAATCCCTGT CCTTGGAGAG 
TTTGTCTCCA GGCCTGTGAA CATCGCCCAG GCTACAGACC GGAGGGATGC CTTTGTCAAG 
GGCATCTATG GGCGCCTCTT CCAGTGGATT GTCAAGAAAA TCAATGCTGC GATCTTCACT 
CCCCAAGCCC AGGATCCCCA AAATGTGCGG AGGGCCATCG GTCTCCTGGA CATATTTGGC 
TTTGAAAACT TCCAGAACAA TAGCTTTGAG CAACTCTGCA TCAACTTTGC CAACGAGCAC 
CTTCAGCAAT TCTTCGTGAA GCACGTGTTC ACGATGGAGC AGGAAGAGTA CCTGTCTGAG 
AACATCACCT GGAACTACAT CCACTACACC GACAACCAGC CCATCCTGGA CATGCTGGCC 
CTCAAGCCCA TGAGCATCAT CTCTCTCCTA GATGAGGAGA GCCGCTTCCC ACAGGGGACA 
GATGTCACTA TGCTGCAGAA ACTGAACAGT ATCCATGCCA ACAACAAGTC CTTCCTGAGC 
CCCAGGAGCA TCCATGACAC CCGATTTGGG ATCGCACACT TCGCGGGCGA TGTATACTAC 
CAGGCAGAAG GCTTTCTGGA GAAGAATCGA GATGTGCTGA GCACAGATAT CCTAATCCTG 
ATTCATTCCT CGAAAAACAA GTTTCTGAAG GAGATATTTA ACGTGGACTC ATCACAGACT 
AAGCTGGGGC ACGGCACCAT CTGCCAGGTG AAGGCAGGAA GCCAGCTCTT CAAGTCATCA 
GACTCCATCA AGCGCCCCGT CACCCTCGCG AGCCAGTTCA AGCAGTCTCT GGACCAGCTG 
ATGAGAATCC TGACCAACTG CCAGCCCTAT TTTGTCCGCT GCATTAAACC CAATGAGTAC 
AAGAAGCCAC TGCTGTTTGA CCGAGAGCTG TGCATTCAGC AGCTGCGTTA TTCCGGCATG 
ATGGAGACTG TGCACATCCG CAAGTCAGGT TTTCCCATCC GCTACACATT TGATGAGTTC 
TCACAGAGAT TCCGTGTGCT GCTGCCCAGC CCGGAGCGTA TGCAGTTTCA GAACAAGCCC 
CGCCAGATGA CCTTGCACAT TGCTGACCTA TGCCTGGGGA CAGACAAAGA CTGGAAAGTC 
GGAAAGACCA AAATCTTCCT GAAGGATCAT CAGGACACAG TGCTGGAGAT CCGCAGGAGC 
CAGGCCCTGG ACGGAGCAGC GATCCGGATC CAGAGAGTCC TCCGGGGACA CAAGTACAGG 
AAGGAGTTCC TGAGGCAGAG ACGTGCAGCT GTGACCCTCC AGGCAGGGTG GCGAGGCTAT 
AGCCAAAGAA AGAATTTCAA GCTGATCCTA GTGGGCTTTG AACGCCTGCA GGCCATCGCC 
CGGAGCCACC TGCTGATGAG ACAGTTCCAG GCCATGCGAC AGAGGATAGT GCAGCTGCAG 
GCCCGATGCA GGGGCTACCT GGTGCGACAG CAAGTCCAGG CCAAGAGGAG GGCAGTGGTG 
ATCATTCAAG CACATGCCAG GGGCATGGTT GTCCGGAAGA GCTACTGGCA ACAGAAAAGC 
ACTGGGCCTC AGGTCATTCT GGCTAAGGAA CCAAAGGCTC AAGTTGCGGT CCATGAGAGG 
AAGCGTAAGT CCATCTATGA CACTGTCACT GACACAGCGA TGGTGGAGAA AGTGTTTGGC 
TTCCTCCCAG CCATGATTGG AGGTCAGGAG GGTCCAGCCC CAACACGCTT TGAGGATCTG 
GAAGTAAAGA CCCAGAAACT TCATGAGGTT GATCTGGATA CAGTGCCCAT GATGGCGATG 
CCAGAAGAGG AGGTGGACAG CCTGGCTGAG TATACCTTCC CCAAGTTTGC TGTGACTTAC 
TTCCAGAAGT CAGCCAGCCA CACCCACATC CAGAAGCCCC TTCGATACCC ACTCCTCTAC 
CATGAAAATG ACACTGACCA CTCGGCTGCT CTAGATGTTT GGATTATCAT CCTGAGGTTC 
ATGGGTGACC TTCCAGAGCC AGTGGTCTAT GGCAGGAACA GCCTGACTGG CAGCTCAGTG 
ATGCGGCAGA TCCATGACAA ACTGGGCAAG GACAGTGTCA CTCAGCACAA CAGATCCTCA 
CAGGTGGCCA GCCAGCTGAA CTTTGGTGAA GAGGCATTCA AGTTTGATGG CCCTATCTCA 
GACAGACCCA TGTCTAACCT AGAGAAGGTG CACTTCATTG TGGGCTACGC CATCATGCGT 
CCTGGACTCA GAGATGAAAT CTACTGTCAG ATCTGCAAGC AGCTCTCCGA GAACTACAAA 
ACAAGTAGCC GGGCCCGGGG CTGGATCCTG CTCAGCCTCT GTCTGGGATG CTTCCCACCC 
TCAGAGAGGT TCATGAAGTA TCTGCTGAAT TTCATTAGTC AAGGGCCACC CAGCTATGGG 
CCCTTCTGTG CTGAACGGCT GCAACGCACC TTCGCCAATG GGGTGCGCGC AGAACCCCCC 
ACCTGGCTGG AGCTGCAGGC TGTCAAGTCC AAGAAGCACA TCCCGATCCA GGTCATCCTG 
GCAACCGGAA GGAGCCTGAC CATCTCTGTG GACTCCGCCT CCACATCACG AGAAATCTGC 
CAGCACGTCG CTCAGAAACA GGGTCTCAGG GACAATCTGG GCTTCTCCCT CCAGGTCGCT 
GTATATGATA AGTTCTGGTC CTTGGGCAGC GGCTGTGACC ATTTGATGGA TGCTGTGGCC 
CAGTGTGAGC AACTGGCCCG AGAGAGGGGA GAGAGCCAGC GGCAGGCCCC CTGGCGTATC 
TACTTCCGGA AGGAGTTCTT CACCCCCTGG CATGACTCCC AGGAGGACCC TGTGAGCACT 
GAGCTCATTT ACCACCAAGT CCTCCGTGGG GTGTGGTCTG GCGAGTATAA CTTTGAGAAG 
GAGGAGGAGC TTGTGGAGCT GCTGGCCCGG CACTGCTATG TGCAGCTTGG GGCCACGGTG 
AAGAGTAACG CTGTCCAGGA GCTGTTGCCC AGCTGTGTTC CCTCCAAACT GTACCGGACC 
AAGTCTCCAG AGAAGTGGGC TAGCCTTGTC ACCGCTGCCC ATGCCAAGGC CCAGTATACC 
CAGTCGAAAG CCACGCCACT GGCGGTGAGG GAGCAGACAG TGGAGGCTGC CCGTCTTCTG 
TGGCCCCTGC TCTTCTCCCG GCTATTTGAA GTCACCACAC TCTCTGGTCC CCGCCTTCCC 
AAGACGCAGC TGGTTTTAGC CATTAACTGG AAGGGGATGT ACTTCCTGGA TCAGAAGGAG 
AGGACACTGC TGGGGTTGTC CTTCGCAGAG GTCATGGGTC TGGTCGCCAA CAGGGACGCC 
CCAGGAGGGA AGAAGCTGCT TCTCGCCACA CTCCAGGAAG AGTACGAGTT CGTCTCCCCC 
AGCAGCGTGG CTATTGCAGA GATGGTGGCC TTGTTCTTGG GAGGCTTGAA GGAGAGATCT 
GTGTTCGCCA TGGCCCTGCA GGACAGAAGG GCCACAGACG ATATCACCCT TCTGCCCTTC 
AAGAAAGGTG ACTTACTGAT CCTGACCAAG AAACAAGGAC TGTTGGCCTC AGAGAACTGG 
GCTCTGGGTC AGAATGACAG AACTGGCAAG ACAGGGCTGG TGCCTACTGC CTGTCTCTAC 
ACCATCCCCT CGGTCACCAA GCCCTCAACC CAGCTGCTGA GCTTGCTGGC CATGTCACCA 
GAGAAACGGA AGCTGGCAGC CCAGGAGGTG CGGGCTCTGG AGCCACCACT GGAGGATCAG 
CTCACAGAGT CGCCATACAC CCTGGAGGAA TTCTCCTACC AGTTCTTCAG GGCTCCGGAG 
AAAGAGACTA TCAGCAGGGC TGCGATGCCC ATGGCCCGAA GCCGGGGTCA TCTCTGGGCC 
TACTCCCCAG AGCCGCTGCG CCAGCCACTG CTCAAATCTG TCCACGACAA AGCCAAACTG 
CGGGATGCAG CCTGCCAGAT CTTCCTTGCC ATCCTCAAGT ACACAGGTGA CTACCCTTCC 
CGCCAGTCCT GGCACAGCCT GGAGCTCACA GACCAGATGT TCTCGTTAGC CCTGCAGGAC 
CCAGCCCTGC AGGATGAGCT GTACTGCCAG ATCCTGAAGC AGCTGACCCA CAACTCCATC 
AGGTTCAGTG AAGAGCGAGC TTGGCAGCTA CTCTGGCTCT GCACAGGTCT CTTCCCACCT 
GGGAAGACAT TGCTGCCCCA CGCTCAGAAG TTCATAGACT CCCGGAAGAA GAAGCCGCTG 
GCTCTCGACT GTAGCCGCAG GCTCCACAGG GTTCTAAGGG TGGGACCTCG GAAGCAGCCC 
CCACATGATG TAGAGGTGAA AGCGGCTGAG CAGAATGTCT CCAAATTACA CCATGAGGTC 
TACCTCCCCA ATGACACCAG CAAGTCCATG GAAGTGGGCA GCAGTAGTCG GGTGAGAGAC 
CTATGTGAGG GCATAGGCAC CAGGCTCCAG CTGGCCTCCT GGGATGGCTG CAGCCTCTTC 
ATCAAGATCA CAGACAAGGT CATCAGTCTG AAGGAAGGAG ACTTCTTTTT TGACAGCTTG 
AGGCAGGTAT CTGACTGGGT GAAGAAGAAC AGGCCCCAGA AAGAAGGGGC CTCCGTGACA 
CTTCCCTATC AGGTGTTCTT CATGCGGAAA CTGTGGCTCA ACGTGACCCC TGGGAAGGAC 
GTGAATGCAG ACACCATACT CCATTACCAC CAGGAACTAC CCAAGTACCT CCGCGGGTTT 
CACAAGTGCT CCCGGGAAGA TGCCATCCAC CTGGGAGGTC TCATCTGTAA AATCCAGTTT 
GGTAGTGACA GCTCGCAACT GGCTAGTGTC TCAAAGGTCC TGAAGGAGCT GGTACCTCAG 
AACCTCACAC GCCTGATGTC CTCAGAAGAA TGGAAAAAGA GCCTCCTTCT GGAATGTGAC 
AAAAACAAGC GCAAGACAGT GGCAGAGGCC AAAGTGGAGT TCTTGAAGTA CATGTACAGG 
TGGCCCACCT TTGGGTCTGC CTTCTTTGAG GTGAAGCAAA CCTCTGAGCC TTCCTACCCA 
GACATTCTCC TCATCGCCAT CAACCGACAT GGGTTACTGC TCATCCACCC CAAGACCAAG 
GAGCTGCTGA ACACCTACCC TTTCACCAAG ATCTCCAGCT GGAGTAGTGG CAACACCTAT 
TTCCACATGG CACTGGGGAG CCTGGGCCAG GGCAGCCGTT TGCTGTGTGA GACCTCTCTG 
GGTTACAAGA TGGACGACCT GCTGACCTCA TATGTACAGC AGCTCCTGAA TACAGTGAAC 
AAACAGCGGG GCTTCAGGGC TCCCGCCCCG GCCAACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Intestinal brush border assembly driven by protocadherin-based intermicrovillar adhesion.
Cell157(2)433-446(2014 Apr)
Crawley SW,Shifrin DA Jr,Grega-Larson NE,McConnell RE,Benesh AE,Mao S,Zheng Y,Zheng QY,Nam KT,Millis BA,Kachar B,Tyska MJ

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Molecular biology of the cell23(2)324-36(2012 Jan)
Revenu C,Ubelmann F,Hurbain I,El-Marjou F,Dingli F,Loew D,Delacour D,Gilet J,Brot-Laroche E,Rivero F,Louvard D,Robine S

Vertebrate myosin VIIb is a high duty ratio motor adapted for generating and maintaining tension.
The Journal of biological chemistry280(47)39665-76(2005 Nov)
Henn A,De La Cruz EM