SMCP cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMCP
Entrez Gene ID	17235
Full Name	sperm mitochondria-associated cysteine-rich protein
Synonyms	Mcs,Mcsp
General protein information	Preferred Names sperm mitochondria-associated cysteine-rich protein Names sperm mitochondrial-associated cysteine-rich protein
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_008574.4	NP_032600.3	sperm mitochondrial-associated cysteine-rich protein
NM_008574.3	NP_032600.2	sperm mitochondrial-associated cysteine-rich protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing.
Vourekas A, Zheng K, Fu Q, Maragkakis M, Alexiou P, Ma J, Pillai RS, Mourelatos Z, Wang PJ
Genes & development29(6)617-29(2015 Mar)

Mechanisms of translational repression of the Smcp mRNA in round spermatids.
Cullinane DL, Chowdhury TA, Kleene KC
Reproduction (Cambridge, England)149(1)43-54(2015 Jan)

Identification of elements in the Smcp 5' and 3' UTR that repress translation and promote the formation of heavy inactive mRNPs in spermatids by analysis of mutations in transgenic mice.
Bagarova J, Chowdhury TA, Kimura M, Kleene KC
Reproduction (Cambridge, England)140(6)853-64(2010 Dec)

Comparative analysis of testis protein evolution in rodents.
Turner LM, Chuong EB, Hoekstra HE
Genetics179(4)2075-89(2008 Aug)

The 5' UTR and 3' UTR of the sperm mitochondria-associated cysteine-rich protein mRNA regulate translation in spermatids by multiple mechanisms in transgenic mice.
Hawthorne SK, Busanelli RR, Kleene KC
Developmental biology297(1)118-26(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smcp mRNA translation is regulated by multiple elements in the 5' UTR and 3' UTR.
Title: Identification of elements in the Smcp 5' and 3' UTR that repress translation and promote the formation of heavy inactive mRNPs in spermatids by analysis of mutations in transgenic mice.

The patterns of transcription and translation of the Smcp promoter and it's 5'UTR and 3' UTR
Title: The 5' UTR and 3' UTR of the sperm mitochondria-associated cysteine-rich protein mRNA regulate translation in spermatids by multiple mechanisms in transgenic mice.

The following SMCP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMCP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18667	NM_008574.3 Latest version!	Mus musculus sperm mitochondria-associated cysteine-rich protein (Smcp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OMu83905	NM_008574.4 Latest version!	Mus musculus sperm mitochondria-associated cysteine-rich protein (Smcp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMCP ( NM_008574.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_032600.2 Mus musculus sperm mitochondria-associated cysteine-rich protein (Smcp), mRNA.

Price & Availability↑

CloneID	OMu18667
Clone ID Related Accession (Same CDS sequence)	NM_008574.3
Accession Version	NM_008574.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Mus musculus(House mouse)
Product	sperm mitochondrial-associated cysteine-rich protein
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU936528.1, BC055104.1 and BY456161.1. On Jul 28, 2006 this sequence version replaced NM_008574.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC055104.1, AK077172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology protein contains selenocysteine :: PMID: 1418626, 8634143, 8833144 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAC TAGACTGCTG AGGAAGACCC AGCATCTATT CAATCTGCTG AAACATCCAG 
GAAACTACTT TTAACACCGA GAATCAAGTA TGGAAATGCT GAACTAAGAA GAGCCCAAGG 
AAGAACTGTG TTGCCAGATC AGGAACTCCA ACTCTAAAGA AGATGAGTGA TCCATCCAAA 
ACCAACCAGT GCCCCCCTCC ATGCTGCCCA CCAAAACCAT GTTGCCCACC TAAACCGTGC 
TGTCCACAGA AACCACCTTG CTGTCCCAAA TCCCCATGCT GCCCACCCAA GTCCCCATGT 
TGCCCTCCAA AGCCCTGTCC CTGTCCTCCC CCCTGTCCCT GTCCCTGTCC AGCCACCTGT 
CCTTGTCCGC TGAAACCACC ATGCTGCCCA CAGAAGTGTT CGTGCTGCCC CAAAAAGTGC 
ACCTGCTGCC CACAGCCACC TCCTTGCTGC GCTCAACCTA CCTGCTGCTC TTCAGAGAAC 
AAGACTGAGT CAGATTCTGA TACATCTGGC CAAACTCTGG AGAAGGGCTC TCAATCACCA 
CAGTCCCCAC CAGGTGCTCA AGGCAACTGG AACCAGAAGA AGTCAAACAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032600.2
CDS	55..648
Translation	MDSLDCGRPSIYSICNIQETTFNTENQVWKC*TKKSPRKNCVARSGTPTLKKMSDPSKTNQCPPPCCPPKPCCPPKPCCPQKPPCCPKSPCCPPKSPCCPPKPCPCPPPCPCPCPATCPCPLKPPCCPQKCSCCPKKCTCCPQPPPCCAQPTCCSSENKTESDSDTSGQTLEKGSQSPQSPPGAQGNWNQKKSNK

Target ORF information:

RefSeq Version	NM_008574.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus sperm mitochondria-associated cysteine-rich protein (Smcp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008574.3

ORF Insert Sequence:

ATGGACTCAC TAGACTGCTG AGGAAGACCC AGCATCTATT CAATCTGCTG AAACATCCAG 
GAAACTACTT TTAACACCGA GAATCAAGTA TGGAAATGCT GAACTAAGAA GAGCCCAAGG 
AAGAACTGTG TTGCCAGATC AGGAACTCCA ACTCTAAAGA AGATGAGTGA TCCATCCAAA 
ACCAACCAGT GCCCCCCTCC ATGCTGCCCA CCAAAACCAT GTTGCCCACC TAAACCGTGC 
TGTCCACAGA AACCACCTTG CTGTCCCAAA TCCCCATGCT GCCCACCCAA GTCCCCATGT 
TGCCCTCCAA AGCCCTGTCC CTGTCCTCCC CCCTGTCCCT GTCCCTGTCC AGCCACCTGT 
CCTTGTCCGC TGAAACCACC ATGCTGCCCA CAGAAGTGTT CGTGCTGCCC CAAAAAGTGC 
ACCTGCTGCC CACAGCCACC TCCTTGCTGC GCTCAACCTA CCTGCTGCTC TTCAGAGAAC 
AAGACTGAGT CAGATTCTGA TACATCTGGC CAAACTCTGG AGAAGGGCTC TCAATCACCA 
CAGTCCCCAC CAGGTGCTCA AGGCAACTGG AACCAGAAGA AGTCAAACAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCP ( NM_008574.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_032600.3 Mus musculus sperm mitochondria-associated cysteine-rich protein (Smcp), mRNA.

Price & Availability↑

CloneID	OMu83905
Clone ID Related Accession (Same CDS sequence)	NM_008574.4
Accession Version	NM_008574.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	sperm mitochondrial-associated cysteine-rich protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY066868.1, BC055104.1 and BY456161.1. On Aug 3, 2017 this sequence version replaced NM_008574.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC055104.1, AK077172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTGATC CATCCAAAAC CAACCAGTGC CCCCCTCCAT GCTGCCCACC AAAACCATGT 
TGCCCACCTA AACCGTGCTG TCCACAGAAA CCACCTTGCT GTCCCAAATC CCCATGCTGC 
CCACCCAAGT CCCCATGTTG CCCTCCAAAG CCCTGTCCCT GTCCTCCCCC CTGTCCCTGT 
CCCTGTCCAG CCACCTGTCC TTGTCCGCTG AAACCACCAT GCTGCCCACA GAAGTGTTCG 
TGCTGCCCCA AAAAGTGCAC CTGCTGCCCA CAGCCACCTC CTTGCTGCGC TCAACCTACC 
TGCTGCTCTT CAGAGAACAA GACTGAGTCA GATTCTGATA CATCTGGCCA AACTCTGGAG 
AAGGGCTCTC AATCACCACA GTCCCCACCA GGTGCTCAAG GCAACTGGAA CCAGAAGAAG 
TCAAACAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032600.3
CDS	266..697
Translation	MSDPSKTNQCPPPCCPPKPCCPPKPCCPQKPPCCPKSPCCPPKSPCCPPKPCPCPPPCPCPCPATCPCPLKPPCCPQKCSCCPKKCTCCPQPPPCCAQPTCCSSENKTESDSDTSGQTLEKGSQSPQSPPGAQGNWNQKKSNK

Target ORF information:

RefSeq Version	NM_008574.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm mitochondria-associated cysteine-rich protein (Smcp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008574.4

ORF Insert Sequence:

ATGAGTGATC CATCCAAAAC CAACCAGTGC CCCCCTCCAT GCTGCCCACC AAAACCATGT 
TGCCCACCTA AACCGTGCTG TCCACAGAAA CCACCTTGCT GTCCCAAATC CCCATGCTGC 
CCACCCAAGT CCCCATGTTG CCCTCCAAAG CCCTGTCCCT GTCCTCCCCC CTGTCCCTGT 
CCCTGTCCAG CCACCTGTCC TTGTCCGCTG AAACCACCAT GCTGCCCACA GAAGTGTTCG 
TGCTGCCCCA AAAAGTGCAC CTGCTGCCCA CAGCCACCTC CTTGCTGCGC TCAACCTACC 
TGCTGCTCTT CAGAGAACAA GACTGAGTCA GATTCTGATA CATCTGGCCA AACTCTGGAG 
AAGGGCTCTC AATCACCACA GTCCCCACCA GGTGCTCAAG GCAACTGGAA CCAGAAGAAG 
TCAAACAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing.
Genes & development29(6)617-29(2015 Mar)
Vourekas A,Zheng K,Fu Q,Maragkakis M,Alexiou P,Ma J,Pillai RS,Mourelatos Z,Wang PJ

Mechanisms of translational repression of the Smcp mRNA in round spermatids.
Reproduction (Cambridge, England)149(1)43-54(2015 Jan)
Cullinane DL,Chowdhury TA,Kleene KC

Identification of elements in the Smcp 5' and 3' UTR that repress translation and promote the formation of heavy inactive mRNPs in spermatids by analysis of mutations in transgenic mice.
Reproduction (Cambridge, England)140(6)853-64(2010 Dec)
Bagarova J,Chowdhury TA,Kimura M,Kleene KC

Comparative analysis of testis protein evolution in rodents.
Genetics179(4)2075-89(2008 Aug)
Turner LM,Chuong EB,Hoekstra HE

The 5' UTR and 3' UTR of the sperm mitochondria-associated cysteine-rich protein mRNA regulate translation in spermatids by multiple mechanisms in transgenic mice.
Developmental biology297(1)118-26(2006 Sep)
Hawthorne SK,Busanelli RR,Kleene KC